蝎毒的抗炎症作用

本研究发现蝎毒对角叉菜胶诱发的大鼠足爪肿，佐剂性关节炎，琼脂肉芽肿具有抑制作用；对二甲苯诱发的小鼠耳廓肿胀及迟发性过敏反应等急、慢性炎症也具有明显抑制作用。     

蛇酒的抗慢性炎症作用

目的：研究蛇酒的抗慢性炎症作用并为临床应用提供理论基础。方法：①佐剂性关节炎法；②琼脂肉芽肿法；③迟发性过敏反应法。结果：蛇酒对大鼠佐剂性关节炎，琼脂肉芽肿，小鼠迟发性过敏反应等慢性炎症有明显的抑制作用。结论：蛇酒有抗慢性炎症作用。     

蚁附蠲痹颗粒镇痛抗炎作用的实验研究

目的观察蚁附蠲痹颗粒的镇痛抗炎作用.方法采用小鼠热板法和扭体法观察其镇痛作用;采用耳片法、毛细血管通透性亢进法、足肿胀法、棉球肉芽肿法及大鼠佐剂性关节炎模型观察其的抗炎作用.结果蚁附蠲痹颗粒对醋酸所致的扭体反应有明显的抑制作用,热板法显示该制剂能明显提高小鼠的痛阈值,其中中剂量组的作用较为明显;蚁附蠲痹颗粒对二甲苯所致的耳廓肿胀、醋酸所致的毛细血管通透性亢进、蛋清所致大鼠足肿胀及小鼠棉球肉芽肿都有明显的抑制作用;蚁附蠲痹颗粒预防性和治疗性给药对佐剂性关节炎模型均既能明显抑制注射局部的早期炎症反应和12 d后的再度肿胀,又能抑制另一后肢迟发性超敏反应引起的足肿胀,作用强度与可的松相似.结论蚁附蠲痹颗粒有显著的镇痛抗炎作用.     

鹅掌藤浸膏镇痛抗炎作用的实验研究

目的：探讨鹅掌藤浸膏口服给药的镇痛和抗炎作用。方法：选用甲醛致痛法、热板法观察鹅掌藤浸膏镇痛作用，选用角叉菜胶致炎法、巴豆油致炎法、棉球肉芽肿和弗氏完全佐剂性关节炎以及腹膜毛细血管通透性实验法观察其抗炎作用。结果：鹅掌藤浸膏能显著提高小鼠热痛阈值，减少甲醛所致大鼠痛反应分值；对角叉菜胶所致大鼠足肿胀有明显的抑制作用，能减轻巴豆油所致小鼠耳廓肿胀度，抑制小鼠棉球肉芽肿的形成，减轻弗氏完全佐剂所致的大鼠佐剂性关节炎肿胀度，对醋酸所致的小鼠毛细血管通透性增加也有明显的抑制作用。结论：鹅掌藤浸膏口服给药有明显的镇痛、抗炎作用。     

骨质增生祛痛膏镇痛抗炎作用的研究

目的：研究骨质增生祛痛膏的镇痛、抗炎作用。方法：采用化学刺激法观察骨质增生祛痛膏的镇痛作用;采用巴豆油引起小鼠耳廓炎症模型、醋酸所致小鼠腹腔毛细血管通透性增高模型、琼脂所致大鼠肉芽肿模型,观察该药的抗炎作用。结果：骨质增生祛痛膏对化学刺激引起的疼痛反应有明显镇痛作用;对巴豆油所致的小鼠耳廓肿胀、醋酸引起的小鼠腹腔毛细血管通透性增高及琼脂所致的大鼠肉芽肿形成均具有明显的抑制作用。结论：骨质增生祛痛膏具有明显的镇痛、抗炎作用。     

骨友灵贴膏药效学研究

目的：研究骨友灵贴膏的抗炎、镇痛和活血化瘀作用方法：采用二甲苯所致小鼠耳廓肿胀和二甲苯所致小鼠皮肤毛细血管通透性增高及过敏性炎症佐剂性关节炎模型,观察骨友灵贴膏的抗炎作用;采用热板法和扭体法研究骨友灵贴膏的镇痛作用;采用小鼠皮下血斑吸收实验,阐明骨友灵贴膏的活血化瘀作用。结果：骨友灵贴膏对二甲苯所致小鼠耳廓肿胀和二甲苯所致小鼠皮肤毛细血管通透性增高等炎症反应具有显著的抑制作用;对过敏性炎症佐剂性关节炎原发病变和继发病变均有显著的改善作用。热板法和扭体法镇痛实验均显示其能显著提高痛阈值。对小鼠皮下血斑吸收有显著的促进作用,能显著减少血斑面积并显著缩短血斑消失时间结论：骨友灵贴膏具有抗炎、镇痛和活血化瘀作用。     

祖师麻提取物的镇痛与抗炎作用研究

目的观察祖师麻提取物的抗炎、镇痛和抗佐剂性关节炎的作用。方法采用醋酸扭体法、小鼠腹腔毛细血管通透性试验、二甲苯致小鼠耳肿胀试验和弗氏完全佐剂致大鼠佐剂性关节炎试验。结果祖师麻提取物0.04、0.08g/kg可明显减少冰醋酸所致小鼠的扭体反应次数和减轻佐剂致原发性大鼠右后足跖肿胀(P<0.05、0.01);祖师麻提取物0.08g/kg能显著抑制冰醋酸致小鼠毛细血管通透性增加(P<0.05)和明显减轻小鼠耳廓肿胀度(P<0.01)。结论祖师麻提取物具有较好的镇痛、抗急性炎症和抗佐剂性关节炎的作用。     

奇任醇的抗炎免疫实验研究

目的研究奇任醇的抗炎作用及对免疫系统的影响。方法建立大鼠佐剂性关节炎模型，观察奇任醇对大鼠足肿胀、脾淋巴细胞增殖、T淋巴细胞亚群的影响；观察奇任醇对小鼠耳廓肿胀、大鼠肉芽肿的抗炎作用；通过碳粒廓清、小鼠迟发型变态反应和血清溶血素水平实验，评价奇任醇对小鼠非特异性和特异性免疫功能的影响。结果奇任醇能够抑制佐剂性关节炎大鼠的足肿胀，增强佐剂性关节炎大鼠脾淋巴细胞增殖能力，调节T淋巴细胞亚群比例；抑制小鼠耳廓肿胀和大鼠肉芽肿增生；抑制正常小鼠特异性免疫功能。结论奇任醇可抑制佐剂性关节炎大鼠的原发性与继发性足肿胀，抑制急、慢性炎症反应，调节机体免疫功能。     

岗梅水提取物抗炎作用的实验研究

目的 研究岗梅水提取物的抗炎作用.方法 采用二甲苯致小鼠耳廓肿胀法、角叉菜胶致大鼠足跖肿胀法、大鼠棉球肉芽肿法和醋酸致小鼠毛细血管通透性增高法观察岗梅水提取物的抗炎作用.结果 岗梅水提取物能明显抑制二甲苯所致的小鼠耳廓肿胀和角叉菜胶所致的大鼠足跖肿胀及炎性组织中PGE2的生成,减少大鼠棉球肉芽肿的形成,同时对醋酸所致小鼠毛细血管通透性增高具有显著抑制作用.结论 岗梅水提取物具有明显抗炎作用.     

筋骨痛消丸抗炎作用及其机制的研究

目的观察筋骨痛消丸抗炎作用,初步探讨其作用机制。方法采用二甲苯致小鼠耳廓肿胀、醋酸致小鼠毛细血管通透法、小鼠琼脂肉芽肿法研究筋骨痛消丸的抗炎作用;通过测定角叉菜胶致大鼠足跖肿胀炎症组织中白细胞介素-1(IL-1)、肿瘤坏死因子(TNF-α)、前列腺素E2(PGE2)、一氧化氮(NO)、丙二醛(MDA)水平。结果筋骨痛消丸高、低剂量组对二甲苯致小鼠耳廓肿胀、醋酸诱发小鼠毛细血管通透性增高、琼脂致小鼠肉芽组织增生均有显著的抑制作用,可明显降低大鼠角叉菜胶足跖肿胀炎症组织内IL-1、TNF-α、PGE2、NO、MDA的水平。结论筋骨痛消丸具有明显的抗炎作用,机制可能与降低IL-1、TNF-α、PGE2、NO、MDA的水平有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.