银屑病患者血清高密度脂蛋白与血液流变学相关的研究

采用磷钨酸镁（PTA－Mg）测定法和LiANG-100型血液粘度仪检测30例银屑病患者血清高密度脂蛋白（HDL－C）及血液流变学指标并与30例健康人作对照。结果显示，银屑病患者HDL－C与血液流变学各项指标值呈明显负相关。通过检测银屑病患者HDL－C浓度可间接判断其血液粘度；调脂治疗对银屑病可能有一定意义。     

Soluble E-selectin serum levels correlate with disease activity in psoriatic patients

E-selectin is an adhesion molecule expressed on vascular endothelial cells in several inflammatory skin diseases, including psoriasis. It is responsible for the adherence between microvascular endothelium and neutrophils, monocytes, eosinophils and subsets of T cells. Soluble E-selectin (sE-selectin) serum levels were measured by ELISA in 32 psoriatic patients before treatment and compared with both post-treatment sE-selectin levels in 16 patients and sE-selectin values in 10 healthy individuals. Soluble E-selectin serum levels were significantly increased in psoriatic patients compared with healthy persons. Moreover, a significant correlation was demonstrated between sE-selectin values and PASI scores. No relationship was found between sE-selectin levels and duration of psoriasis. Soluble E-selectin serum levels decreased significantly after treatment of psoriasis. This phenomenon was more evident in patients with more severe psoriasis. In conclusion, sE-selectin serum levels correlate with the extent of psoriatic lesions and could be used as marker of the disease activity in psoriatic patients.     

MTX对银屑病患者血清白介素-8和组胺水平的影响

目的:探讨甲氨蝶呤(MTX)对银屑病患者血清白介素-8和组胺水平的影响。方法:用酶联免疫吸附实验(ELISA)检测银屑病患者和正常人外周血IL-8水平;用荧光分光光度计测定银屑病患者和正常人外周血组胺水平。结果:(1)治疗前银屑病患者血清IL-8和组胺水平明显高于正常对照组(P<0.01),经MTX治疗后,患者血清IL-8和组胺水平与正常对照组差异无显著性(P>0.05)。结论:抑制组胺的释放和IL-8的分泌,降低中性粒细胞和T淋巴细胞的趋化活性,减轻炎症反应及组织损伤可能是MTX治疗银屑病的作用机制之一。     

Detection of inflammatory cytokines in psoriatic skin

Recent investigations have revealed the involvement of cytokines in the pathogenesis of psoriasis. This study examined the amount of inflammatory cytokines — interleukin-1 (IL-1), interleukin-6 (IL-6) and granulocyte macrophage colony-stimulating factor (GM-CSF) — released into the supernatants of organ cultures of involved and uninvolved skin from psoriatic patients and normal skin from healthy individuals. Bioassays were employed to detect the activities of IL-1 and IL-6. Enzyme-linked immunosorbent assay (ELISA) methods were used to quantitate immunoreactive IL-1, IL-1, IL-6 and GM-CSF. The activity of IL-1 in uninvolved psoriatic skin was found to be increased relative to that in involved and normal skin, while immunoreactive IL-1 was found only in involved skin. A neutralization experiment showed that bioactive IL-1 was mostly attributable to IL-1. Uninvolved psoriatic skin also secreted higher amounts of both bioactive and immunoreactive IL-6 compared with involved skin. Immunoreactive GM-CSF was detected in uninvolved skin only. These cytokines detected in uninvolved skin may have been released from epidermal or mesenchymal cells, since uninvolved skin contained fewer inflammatory infiltrates. Our results offer additional evidence that increased amounts of inflammatory cytokines in uninvolved skin may provide a preliminary condition and play important roles in the initial events in the evolution of psoriatic lesions.Part of this work was presented in abstract form at the Fifth International Psoriasis Symposium in San Fransisco, 10–14 July 1991     

阿维A对银屑病患者血清血小板衍生生长因子-BB和干扰素诱导蛋白-10的影响

目的:了解阿维A对银屑病患者血清中某些细胞因子水平的影响.方法:采用酶联免疫分析法检测血清血小板衍生生长因子-BB(PDGF-BB)和干扰素诱导蛋白-10(IP-10)水平.结果:银屑病患者在治疗前血清PDGF-BB和IP-10水平均比对照组高(P＜0.01).阿维A治疗后血清PDGF-BB和IP-10水平均显著下降(P<0.01).结论:阿维A对银屑病患者血清中PDGF-BB和IP-10的产生有显著抑制作用,为其治疗银屑病提供理论基础.     

Neurotransmitter imbalance in serum of psoriatic patients in exacerbation stage with comorbid psychoemotional disorders

The role of psychological factors in the manifestation and exacerbation of psoriasis is well known. Acute and chronic stress, anxiety, and depression affect the reactions of innate and acquired immunity. The exacerbations of psoriatic lesions are accompanied by increased production of inflammatory mediators, which could contribute to the imbalance of neurotransmitters and the development of symptoms of depression and anxiety. The role of inhibitory neurotransmitters in the serum of patients with psoriasis exacerbation with comorbid emotional disorders is assessed. Forty patients with psoriasis in the exacerbation stage were examined. Spielberger State‐Trait Anxiety Inventory (STAI) and the Beck Depression Inventory (BDI) scale were used to assess anxiety and depression parameters. We also evaluated the concentration of serotonin and gamma‐aminobutyric acid (GABA) in the serum. Patients with psoriasis had significant anxiety and depressive disorders. An imbalance in serotonin levels and a decrease in serum GABA levels in patients with psoriasis in the acute stage were noted. Only patients with anxiety disorder had increased serotonin levels. Our findings suggest that the imbalance of neurotransmitters in patients with psoriasis in the acute stage is important in predicting the development of the emotional well‐being.     

银屑病患者皮损及鳞屑中人乳头瘤病毒的检测

目的 检测银屑病患者皮损中人乳头瘤病毒(HPV) DNA,探讨其与银屑病的关系。方法 选取3组通用引物,用聚合酶链反应(PCR)检测银屑病活检标本(62份)、皮损处刮屑标本(79份)、非皮损处刮屑标本(62份)、正常人对照的活检标本(48份)和刮屑标本(48份)中的HPV DNA。结果 疣状表皮发育不良相关性人乳头瘤病毒(EV HPV)的DNA检出率在银屑病皮损活检标本中为77.4%,而在正常人活检标本中为27.1%,差异有显著性(P < 0.005)。在银屑病患者的皮损和非皮损处的刮屑中,EV HPV DNA检出率分别为51.9%和48.4%。而正常人刮屑标本中为18.8%,与前两者差异均有显著性(P < 0.005)。在所有标本中均未检测到嗜黏膜性HPV DNA。 结论 银屑病患者中EV HPV DNA的检出率远高于正常人。     

寻常性银屑病皮损表皮中Smad7表达的检测及其临床意义

目的:探讨Smad7基因及其蛋白在寻常性银屑病皮损区表皮中的表达及其意义。方法:采用逆转录(RT)-PCR和SP免疫组化法分别检测寻常性银屑病皮损和正常对照皮肤中Smad7的表达。结果:寻常性银屑病皮损中Smad7表达水平上调。与正常对照皮肤相比,寻常性银屑病皮损区表皮角质形成细胞Smad7的免疫组化染色显著增强(P<0.01)。结论:寻常性银屑病皮损区表皮Smad7的过度表达可能是通过阻断转化生长因子(TGF)-β信号转导,从而有助于银屑病皮损区表皮过度增生。     

Narrow band ultraviolet B irradiations cause alteration in interleukin-31 serum level in psoriatic patients

Scientific communications indicate the disturbed expression of neuropeptides in the skin and serum in psoriasis vulgaris (PsV) patients. Narrow-band ultraviolet radiation (NB-UVB) is one of the systemic therapies of PsV. The aim of the study was to evaluate the influence of NB-UVB therapy on substance P (SP), calcitonin gene-related peptide (CGRP), brain-derived neurotrophic factor (BDNF), corticotropin-releasing factor (CRF) and interleukin-31 (IL-31) serum concentrations in PsV patients. 59 psoriatic patients with mean PASI (psoriasis area and severity index) 14.3 were treated with NB-UVB (20 exposures). The control group consisted of 50 healthy subjects, whose age and sex matched. In all patients, serum concentration of BDNF, CRF, IL-31 substance P and CGRP was analyzed by ELISA before the treatment and in psoriatic group the analysis was also done after 10 and 20 irradiations. In patients there was found a significantly higher concentration of IL-31 (215.3 vs. 748.6 ng/ml; p < 0.0001), SP (25.7 vs. 67.2 pg/ml; p < 0.01), CGRP (31.4 vs. 44.15 pg/ml; p < 0.01) and a lower concentration of CRF (0.89 vs. 0.426 ng/ml; p < 0.0001) and BDNF (16.39 vs. 14.15 ng/ml; p = 0.1216) in comparison with the controls. 20 NB-UVB exposures caused a significant decrease in IL-31 level (748.6 vs. 631.7 ng/ml; p < 0.0001). The NB-UVB therapy had no major effect on neuropeptides serum levels regardless of a number of irradiations. On the basis of our study it can be suggested that IL-31 is involved in pathogenesis of psoriasis and the NB-UVB therapy causes alterations in its level.     

特应性皮炎外周血白介素17水平检测

目的: 检测特应性皮炎(AD)患者血中白介素17(IL-17)水平.方法: 采用双抗体夹心ELISA法检测66例特应性皮炎患者和50名正常对照者血中IL-17含量.结果: 患者组血中IL-17水平明显高于对照组(P<0.001).重度组高于中度组(P<0.001),中度组与轻度组无统计学差异.结论: IL-17可能在AD患者的免疫发病机制中发挥作用.     

寻常型天疱疮患者血清骨桥蛋白的检测北大核心CSCD

目的 探讨寻常型天疱疮（PV）患者血清中骨桥蛋白在PV发病中的作用.方法 用酶联免疫吸附试验对31例寻常型天疱疮患者和35例健康对照组血清骨桥蛋白的浓度进行测定.用SPSS17.0及Excell统计软件对数据进行处理.结果 PV患者血清骨桥蛋白的表达水平显著高于健康对照组（P＜0.01）.皮肤黏膜同时受累患者血清骨桥蛋白水平高于仅皮肤受累组（P＜0.05）.合并感染组血清骨桥蛋白水平高于未合并感染组（P＜ 0.01）; PV患者的血清骨桥蛋白水平与抗Dsg3抗体呈正相关（rs=0.489,P＜ 0.01）.结论 骨桥蛋白在PV患者血清中高表达,特别是皮肤黏膜同时受累的患者,且与天疱疮抗体滴度呈正相关.     

慢性湿疹和荨麻疹患者血清过敏原检测

报告了2000年3月－2001年3月对128例慢性湿疹，慢性荨麻疹患者进行血清过敏原特异性IgE抗体检测，并与40名正常人对照。结果128例患者血清过敏原反应阳性97例，对一种过敏原呈阳性反应24例，对2种以上过敏原呈阳性反应73例。阳性率较高的过敏原是屋尘，尘螨，多价霉 ，牛羊肉。40名正常人血清过敏原反应全部阴性。血清总IgE值：128例患者有94例＞50IU／mL，对照组均＜20IU／mL。     

Detection of cross-reactive idiotypes in the serum of patients with bullous pemphigoid

To further characterize the circulating antibasement membrane zone (antiBMZ) antibodies present in the sera of patients with bullous pemphigoid (BP), we have generated a mouse monoclonal anti-idiotypic antibody (antiId 3-17) specific for an IgG antiBMZ antibody. AntiId 3-17 is specific for an idiotype expressed on antiBMZ IgG in the serum of a patient with BP, and not expressed on pooled normal human IgG or IgG from patients with other autoimmune skin diseases. AntiId 3-17 binds to non-reduced, but not reduced, antiBMZ IgG on immunoblot, suggesting that the idiotype is composed of a conformational epitope expressed on native antibody. By a competitive inhibition ELISA, antiId 3-17 detects a cross-reactive idiotype (CRI) expressed in 18 of 50 (36%) of the sera of patients with BP, but in the sera of only 1 of 50 (2%) normal blood bank controls (p less than 0.001, Fisher's exact test) and 1 of 12 (8%) patients with pemphigus (p = 0.005). Thus, antiId 3-17 recognizes a public idiotype on a native antiBMZ antibody from a patient with BP, which is expressed in the sera of 36% of the unrelated patients with BP studied.