Effect of insulin on the expression of interleukin 2 receptor

Summary In this paper, we report our work about the effect of insulin on the expression of interleukin 2 receptor (IL-2R). Our experiments showed that insulin was not T cell growth factor, but was able to augment the expression of IL-2R during T cell activation by PHA and to delay the decline rate of IL-2R⁺ cell. As a result, the percentage of IL-2R⁺ cells significantly increased during and after activation. Furthermore, insulin was capable of enhancing the production of interleukin 1 by macrophage and interleukin 2 by T lymphocyte. Based on these results, we suggest that there are at least two related mechanisms involved in the augmentation of the expression of IL-2R on activated T cells, i.e., direct action of insulin on T cells and indirect action of insulin by enhancing the production of interleukin l.     

Changes of intracellular IP3 with the expression of interleukin 2 receptor in human peripheral blood T lymphocytes

Summary The changes of the intracellular inositol-l,4,5-triphosphate (IP₃) associated with the expression of the interleukin 2 receptor (IL-2R) were studied. In resting lymphocytes, IL-2 did not alter intracellular concentration of IP₃, but Con A caused an increase in IP₃ by 45%. In IL-2 sensitive T cells, which expressed IL-2R by 83 %, the change of intracellular IP₃ was dependent upon IL-2 concentration. The IP₃ increased at IL-2 concentrations of 10 and 50 U/ml, and the maximal response of 60 % was found at the concentration of 50 U/ml. At IL-2 concentration of 100 U/ml no increase in IP₃ was observed. After binding of anti-Tac Me Ab to IL-2R of T lymphocytes the increase in IP₃ at IL-2 concentrations of 10 and 50 U/ml was significantly attenuated. It has been suggested that IL-2 could induce the changes of intracellular IP₃ of the human peripheral blood T cells, which is related to the IL-2 concentrations incubated with T cells and the expression of IL-2R on T lymphocyte.     

可溶性白细胞介素－2受体在体外对白细胞介素－2介导免疫反应的抑制

用酶联免疫吸附法￣３Ｈ－ＴｄＲ掺入法探讨了可溶性白细胞介素－２受体（Ｓｏｌｕｂｌｅｉｎｔｅｒｌｅｕｋｉｎ２ｒｅｃｅｐｔｏｒ，ｓＩＬ－２Ｒ）与ｒＩＬ－２的结合特性以及ｓＩＬ－２Ｒ对ＩＬ－２依赖性ＣＴＬＬ－２细胞、ＰＨＡ激活的正常人外周血淋巴细胞增殖反应的作用，结果显示ｓＩＬ－２Ｒ保持了与ｒＩＬ－２结合的特性。在体外ｓＩＬ－２Ｒ对ＰＨＡ诱导正常人ＰＢＬ增殖以及ＣＴＬＬ－２细胞、ＰＨＡ－ＰＢＬ对ＩＬ－２依赖性增殖均有明显的抑制作用，尤其高浓度的ｓＩＬ－２Ｒ抑制作用更为突出。     

阻塞性黄疸患者外周血白细胞介素2产生和白细胞介素2受体表达的变化

观察31例阻塞性黄疸患者淋巴细胞增殖能力、白细胞介素2(IL-2）产生及白细胞介素2受体（IL-2R）表达的变化。结果发现：阻塞性黄疸患者淋巴细胞增殖能力较正常对照组明显下降（P＜0.001），且这一异常与患者外周血单个核细胞IL-2产生及IL-2R表达受抑制有显著相关性（P＜0.05、＜0.01）。结果表明：阻塞性黄疸患者存在着T细胞介导的免疫功能异常，而IL-2产生减少和IL-2R表达受抑是淋巴细胞增殖受损及感染易感性增高的重要原因。     

慢性酒精中毒性精神障碍患者血清sIL-2R的测定

目的　探讨慢性酒精中毒性精神障碍患者血清中可溶性白细胞介素 2受体 (sIL 2R)水平的变化及其意义。方法　用双抗夹心ELISA法对 32例慢性酒精中毒性精神障碍患者和 30例健康对照血清中sIL 2R水平进行测定。结果　慢性酒精中毒性精神障碍患者血清中sIL 2R水平 (438.75± 92 .5 4 μ/ml)高于对照组 (2 73.6 7± 4 9.4 3μ/ml) (P <0 .0 0 1) ,两组个体间有部分重叠。结论　慢性酒精中毒性精神障碍患者血清sIL 2R水平增高表明有免疫功能抑制。     

滋养细胞肿瘤患者血清可溶性白细胞介素-2受体测定

应用双抗体夹心酶联免疫吸附试验（ＥＬＩＳＡ）和放射免疫分析法（ＲＩＡ）测定了５１例滋养细胞肿瘤患者及２８名正常非孕妇女血清可溶性白细胞介素２受体（ＳＩＬ２Ｒ）和人绒毛膜促性腺激素β亚单位（ＨＣＧβ）水平，并将ＳＩＬ２Ｒ与ＨＣＧβ进行直线相关分析。结果：肿瘤患者血清ＳＩＬ２Ｒ与正常对照组比较差异不显著（Ｐ＞００５），而ＨＣＧβ则明显高于正常对照组（Ｐ＜００１）；相关分析ｒ＝０１７３５，Ｐ＞００５。结论：该患者血清ＳＩＬ２Ｒ的低水平可能是高水平的ＨＣＧβ所致     

可溶性白介素2受体与肿瘤坏死因子在胸腔积液中的表达

目的　了解结核和恶性胸水中可溶性白介素 2受体 (sIL 2R)与肿瘤坏死因子 (TNF)水平及其临床意义。方法　采用双抗体夹心ELISA法和放射免疫分析法 (RIA法 )检测 2 6例结核性、2 2例恶性胸腔积液中sIL 2R与TNF浓度。结果　结核性胸水中sIL 2R与TNF含量分别为 (1310 .4 0± 36 7.89)U ml,(1.79± 0 .5 8) μg L ;恶性胸水中sIL 2R与TNF含量分别为 (94 3.2 6± 2 17.74 )U ml,(1.2 9± 0 .5 1) μg L ;结核性胸水组sIL 2R与TNF浓度均明显高于恶性胸水组 ,两组间有显著性差异 (P <0 .0 0 1,P <0 .0 0 5 ) ;联合检测二者诊断结核性胸腔积液的敏感度、特异度、正确度分别为 10 0 %、96 %、98%。结论　检测胸水中sIL 2R与TNF含量有助于结核性和恶性胸腔积液的鉴别     

荷瘤小鼠脾细胞诱生IL_2活性的动态观察

本文观察了Lewis肺癌荷瘤小鼠脾细胞IL_2和Con A转化水平的动态变化。结果表明,随着荷瘤量增加,IL_2、Con A转化水平和胸腺系数均呈线性下降趋势(r分别为-0.8431、-0.8654和-0.9037,P均小于0.01),且IL_2和胸腺系数的下降发生在解剖后局部出现可见瘤块之前(P值分别小于0.001和0.05)。此外,本文初步检测了荷Lewis肺癌14日小鼠脾细胞Con A活化后,于上清中出现的IL_2 INH活性。并对荷瘤小鼠脾细胞IL_2活性降低、较高水平的IL_2 INH活性的存在、较低的Con A转化水平和胸腺萎缩等因素之间的关系作了讨论,有待于进一步探讨。     

Changes and clinical significances of serum soluble interleukin-2 receptor in patients with mechanical trauma

Objective: To study the changes of serum level of soluble interleukin-2 receptor (sIL-2R) in patients with mechanical trauma and the relationship between sIL-2R serum level and injury severity score (ISS), and to explore the mechanism of changes and clinical significances of sIL-2R. Methods: According to the criterion of ISS, 50 patients with mechanical trauma were divided into mild degree group, severe degree group and extremely severe degree group. Twenty-five of those were dynamically observed for 12 d. Blood samples of all the patients were obtained within 24 h, and ond3,d5,d7,d9 and d 12 in dynamically observed patients. The serum concentration of sIL-2R was assayed by ELISA. Results: Level of sIL-2R was significantly higher in patients with trauma than that in healthy persons within 24 h (P<0. 05). Dynamical observation showed curving changes of sIL-2R with increasing in early stage and decreasing later. The climax of sIL-2R appeared earlier in mild group than that in severe degree group and extrem     

滨蒿内酯对哮喘豚鼠脾淋巴细胞转化和IL-2生成的影响

目的：研究哮喘豚鼠脾淋巴细胞转化和白细胞介素2（IL-2）含量的变化及滨蒿内酯（Sco）对其的影响。方法：建立卵蛋白致敏的哮喘豚鼠模型。MTT比色分析法测定光密度值，比较不同浓度Sco对淋巴细胞增殖、植物血凝素（PHA）诱导的T淋巴细胞转化以及脾细胞分泌IL-2活性的影响。结果：Seo剂量依赖地对哮喘豚鼠淋巴细胞增殖有抑制作用；Seo能够抑制PHA诱导的哮喘朦鼠T淋巴细胞转化；哮喘豚鼠IL-2生成水平高于正常对照组。Seo也可抑制哮喘豚鼠IL-2的活性。结论：Seo在体外抑制哮喘豚鼠脾淋巴细胞转化和脾淋巴细胞产生IL-2的水平，可能是其治疗哮喘的免疫学机制之一。     

恶性肿瘤放疗前后血清可溶性白细胞介素2受体含量变化的临床意义

目的　探讨血清可溶性白细胞介素２ 受体（ｓ Ｉ Ｌ２ Ｒ）水平与恶性肿瘤患者的病期及疗效的关系。方法　采用双抗体夹心 Ｅ Ｌ Ｉ Ｓ Ａ 法检测１５９ 例恶性肿瘤患者放疗前后血清ｓ Ｉ Ｌ２ Ｒ 水平。结果　恶性肿瘤患者放疗前血清ｓ Ｉ Ｌ２ Ｒ 水平明显高于正常对照组（ Ｐ＜ ００５）;放疗后血清ｓ Ｉ Ｌ２ Ｒ 水平明显低于放疗前（ Ｐ ＜ ０００１）;晚期患者（Ⅲ＋ Ⅳ期）不论是放疗前或放疗后 ｓ Ｉ Ｌ２ Ｒ 水平均明显高于早期患者（Ⅰ＋ Ⅱ期）（ Ｐ ＜ ００５）;各类恶性肿瘤之间血清ｓ Ｉ Ｌ２ Ｒ 水平无显著性差异（ Ｐ ＞００５）。结论　ｓ Ｉ Ｌ２ Ｒ水平在各种恶性肿瘤中的表达无特异性;检测恶性肿瘤患者ｓ Ｉ Ｌ２ Ｒ 放疗前后水平,是对病情估计和治疗疗效评价的一项参考指标。     

慢性乙型肝炎病人血清中游离白细胞介素2受体升高的意义

应用放射免疫分析法测定慢性活动性肝炎(CAH)和慢性迁延性肝炎(CPH)病人及健康人血清中游离白细胞介素2受体(fIL2R)水平,CAH和CPH病人的cpm分别是2326±617及1277±397,健康人仅为343±213。结果发现:CAH和CPH病人血清中fIL2R水平均显著高于健康人(P<0.01);CAH病人血清中fIL2R水平高于CPH病人(P<0.01)。提示,慢性乙型肝炎病人血清中fIL2R水平升高可能与其免疫应答异常及炎症活动性有关。     

SIL-2R在肝癌患者血清中的表达及其临床意义的研究

目的:明确原发性肝癌患者血清中可溶性白细胞介素2受体(SIL—2R)水平与肿瘤的关系。方法:采用双抗体夹心ELISA法.对32例原发性肝癌患者手术前后血清SIL—2R进行检测。结果:1.肝癌患者术前血清SIL—2R水平明显高于健康成人(P<0.01)。2.肝癌肿瘤体积大小与血清SIL—2R水平升高幅度有关(P<0.05)。3.手术后经化疗和介入治疗9个月肿瘤无转移有血清SIL—2R水平低于术前(P<0.05),而有转移者血清SIL—2R水平明显高于术前(P<0.01)。结论:对原发性肝癌患者血清SIL—2R水平测定,不仅能了解肿瘤患者的机体免疫功能状态,且在一定程度上能对疾病的严重程度、疗效及判断预后作出评估。     

可溶性白细胞介素-4受体在哮喘小鼠肺组织中的变化及地塞米松的干预作用

目的　探讨可溶性白细胞介素 4受体 (sIL 4R)在哮喘发病中的作用及地塞米松(DXM)对sIL 4R的影响。方法　用卵清白蛋白复制BALB/c小鼠哮喘模型 ,RT PCR方法检测哮喘小鼠基础的和DXM干预后肺组织sIL 4RmRNA的表达变化。结果　哮喘小鼠支气管管壁及血管周围大量炎性细胞浸润 ,以嗜酸性粒细胞 (EOS)为主。经DXM干预后肺组织中炎性细胞浸润明显减少。哮喘组小鼠的sIL 4RmRNA相对丰度显著下调。经DXM干预后 ,sIL 4R基因相对丰度显著增高。结论　支气管哮喘急性发作时 ,存在sIL 4RmRNA表达下调 ;DXM可纠正sIL 4RmRNA的表达下调。     

87例严重肝外伤手术体会

目的 探讨提高外科治疗严重肝外伤的成功率的相应方法和措施。方法 回顾性分析1985年1月～2004年10月收治的87例严重肝外伤的伤情、手术方法及效果。结果 按AAST对肝脏损伤的分级，Ⅲ级外伤48例，Ⅳ级外伤25例，Ⅴ级外伤14例，腹腔内出血量600～2400mL，平均1700mL。手术治愈66例，死亡21例（失血性休克14例，复合伤、多脏器功能衰竭各5例，严重感染致ARDS2例）。结论 正确而及时的诊断，减少术前时间，合理选择手术救治方法，是提高严重肝外伤治疗成功的关键。     

芍药甙和去芍药甙白芍总甙对佐剂性关节炎大鼠脾淋巴细胞产生白介素2的影响

用亚适量刀豆素Ａ（３ｍｇ·Ｌ－１）诱导佐剂性关节炎（ＡＡ）大鼠脾淋巴细胞产生白介素２（ＩＬ－２）。福氏完全佐剂致炎后ｄ１８，ＡＡ大鼠脾淋巴细胞产生ＩＬ－２显著低于正常对照大鼠，芍药甙（ＰＦ）、去芍药甙白芍总甙（ＴＧＰＲＰＦ）和白芍总甙（ＴＧＰ）促进ＡＡ大鼠脾淋巴细胞产生ＩＬ－２，其量效曲线均呈钟罩形，３种受试物显著促进作用的浓度范围为：ＰＦ２．５～１２．５ｍｇ·Ｌ－１，ＴＧＰＲＰＦ１２．５～６２．５ｍｇ·Ｌ－１，ＴＧＰ２．５～６２．５ｍｇ·Ｌ－１。这些结果表明：ＰＦ、ＴＧＰＲＰＦ和ＴＧＰ对ＡＡ大鼠脾淋巴细胞产生ＩＬ－２均具有浓度和机能依赖性的调节作用     

糖皮质激素受体在多发伤后早期作用的研究

目的从组织细胞受体水平探讨糖皮质激素受体(GR)在多发伤中的作用。方法50例小鼠随机分成多发伤组(40例)和对照组(10例),建立多发伤小鼠动物模型。采用逆转录-聚合酶链反应(RT-PCR)法检测GRmRNA表达水平,同时检测血清丙氨酸转胺酶(ALT)、肌酐(Cr)水平,肺组织髓过氧化物酶(MPO)和小肠组织二胺氧化酶(DAO)活性。结果多发伤伤后6、24、72h的血、肝、肾、肺、小肠组织GRα表达明显降低,而GRβ明显高于对照组(P<0.05)。且多发伤组ALT、Cr和MPO升高,而DAO活性降低(P<0.05)。结论GR可能在多发伤早期器官组织的损伤与抗损伤机制方面起着重要作用。     

四物汤对小鼠脾细胞分泌IL-3和IL-2的促进作用

目的:研究四物汤对小鼠脾细胞分泌IL-3和IL-2的促进作用.方法:用3H-TdR掺入法和斑点杂交法,考察四物汤对刀豆球蛋白A(Con A)诱导的小鼠脾淋巴细胞分泌IL-3和IL-2的影响.结果:四物汤可显著促进Con A诱导的脾淋巴细胞分泌IL-3和IL-2(P＜0.01,P＜0.05),促进淋巴细胞中IL-3 mRNA的表达.结论:四物汤能增强小鼠的造血功能.     

KG881抑制致分裂素诱导的淋巴细胞增殖和白介素1的生成

ＫＧ８８１均能抑制亚适量刀豆素Ａ（３ｍｇ．Ｌ＾－１）诱导的小鼠脾脏Ｔ淋巴细胞增殖及适量脂多糖诱导的小鼠脾脏Ｂ淋巴细胞增殖和大鼠腹腔巨噬细胞产生白素１，其抑制作用均呈浓度依赖性。上述结果表明，ＫＧ８８１对致分裂素诱导的淋巴细胞增殖和白介素１产生具有抑制作用。     

Interleukin 2 production and its relationship with T lymphocyte subsets in patients with obstructive jaundice

Summary Sepsis remains a major risk in the high mortality and morbidity after surgery for obstructive jaundice. The reasons for the increased susceptibility to infection are unknown. This study examined interleukin 2 (IL-2) production and the lymphocyte response to PHA mitogen in 31 patients with obstructive jaundice. Among them, 18 patients were simultaneously investigated by enumeration of T lymphocyte subsets in peripheral blood with APAAP technique. The results showed that the patients had significantly decreased IL-2 production and lymhocyte response to PHA mitogen. The percentage of Leu 3a (helper/inducer T cell) in the patients was significantly lower than that in normal controls. Leu 3a/Leu 2a (suppressor/cytotoxic T cell) ratio was significantly lower in these patients. The reduction of IL-2 production correlated significantly with the suppression of lymphocyte proliferation but not with the percentage of Leu 3a cells. From these results, it may be suggested that the reduction of IL-2 production in the patients with obstructive jaundice is an important reason for the suppression of T lymphocyte proliferative response, not merely a reflection of the decrease of helper T cells. This study was supported by a Grant No. 39000100 from National Natural Sciences Foundation of China and by a Grant from the Youth Science Foundation from the Ministry of Public Health of China.