A comparison of critical force and electromyographic fatigue threshold for isometric muscle actions of the forearm flexors

The purpose of this study was twofold: (1) to determine if the mathematical model used for estimating the EMG_FT during cycle ergometry was applicable to isometric muscle actions; and (2) to compare the mean torque level from the CF test to that of the EMG_FT test. The CF was defined as the slope coefficient of the linear relationship between total “isometric work” (W _lim in N m s) and time to exhaustion (T _lim). The EMG_FT was defined as the y-intercept of the isometric torque versus EMG fatigue curve slope coefficient relationship. There was a significant (p < 0.05) mean difference between CF (6.6 ± 3.2 N m) and EMG_FT (10.9 ± 4.7 N m). The results of the present study suggested that, during isometric muscle actions of the forearm flexors, fatigue thresholds estimated from the W _lim versus T _lim relationship (CF) are different from those estimated from electromyographic fatigue curves (EMG_FT).     

Flywheel resistance training calls for greater eccentric muscle activation than weight training

Changes in muscle activation and performance were studied in healthy men in response to 5 weeks of resistance training with or without “eccentric overload”. Subjects, assigned to either weight stack (grp WS; n = 8) or iso-inertial “eccentric overload” flywheel (grp FW; n = 9) knee extensor resistance training, completed 12 sessions of four sets of seven concentric–eccentric actions. Pre- and post-measurements comprised maximal voluntary contraction (MVC), rate of force development (RFD) and training mode-specific force. Root mean square electromyographic (EMG_RMS) activity of mm. vastus lateralis and medialis was assessed during MVC and used to normalize EMG_RMS for training mode-specific concentric (EMG_CON) and eccentric (EMG_ECC) actions at 90°, 120° and 150° knee joint angles. Grp FW showed greater (p < 0.05) overall normalized angle-specific EMG_ECC of vastii muscles compared with grp WS. Grp FW showed near maximal normalized EMG_CON both pre- and post-training. EMG_CON for Grp WS was near maximal only post-training. While RFD was unchanged following training (p > 0.05), MVC and training-specific strength increased (p < 0.05) in both groups. We believe the higher EMG_ECC activity noted with FW exercise compared to standard weight lifting could be attributed to its unique iso-inertial loading features. Hence, the resulting greater mechanical stress may explain the robust muscle hypertrophy reported earlier in response to flywheel resistance training.     

Surface EMG signal alterations in Carpal Tunnel syndrome: a pilot study

Fatigability and muscle oxygen consumption (mVO₂) during sustained voluntary isometric knee extensions are less at extended (30° knee angle; 0°, full extension) versus flexed knee angles (90°). This lower energy consumption may partially result from lower neural activation at extended knee angles. We hypothesized a smaller difference in mVO₂ between extended and flexed knee angles during electrical stimulation, which guaranteed maximal activation, than during maximal voluntary contractions (MVC). In eight healthy young males, MVC extension torque was obtained at 30°, 60° and 90° knee angles. mVO₂ of the rectus femoris (RF), vastus lateralis (VL) and medialis muscle was measured using near-infrared spectroscopy during tetanic (10 s) and maximal voluntary (15 s) contractions (MVC₁₅). For electrically induced contractions, steady state mVO₂ was reached at similar (P > 0.05) times after torque onset (4.6 ± 0.7 s) at all knee angles. In contrast, during MVC₁₅ at 30° mVO₂ was reached at 7.1 ± 1.1 s, significantly later compared to 60° and 90° knee angles. The knee angle dependent differences in mVO₂ were not lower in electrically induced contractions (as hypothesised) but were similar as in voluntary contractions. Normalized mVO₂ at 30° (percentage 90° knee angle) was 79.0 ± 9.4% (across muscles) for electrically induced and 79.5 ± 7.6% (across muscles) for voluntary contractions (P < 0.05). We conclude that the slower onset of mVO₂ during voluntary effort at 30° may have been due to a lower maximal activation. However, because steady state mVO₂ both during electrically induced and voluntary contractions was ~20% less at extended versus flexed knee angles, the causes for the lower mVO₂ must reside within the muscle itself.     

Decline in voluntary activation contributes to reduced maximal performance of fatigued human lower limb muscles

In upper limb muscles, altered corticospinal excitability and reduction in neural drive are observed in parallel with peripheral fatigue during prolonged and/or repeated contractions. However, the fatigue-induced adaptations of central and peripheral elements and their relative contribution to lower limb muscle performance are yet to be fully explored. In the present study, corticospinal excitability and peripheral contractility of ankle flexor muscles were quantified before, during and after repeated brief unilateral maximal dorsiflexions to fatigue in eleven healthy volunteers. Transcranial magnetic stimulation of the motor cortex area related to lower limb muscles was performed, and the evoked twitch and EMG responses in tibialis anterior (TA) and soleus (SOL) were measured. The motor evoked potentials (MEPs) in fatigued TA during post-exercise maximal dorsiflexions were smaller (?20?±?6?%, p?=?0.026) and remained depressed for at least 5?min. Post-exercise MEPs in fatigued SOL and silent periods in TA and SOL were not different compared to pre-exercise. These changes were accompanied by lower voluntary torque (?8?±?3?%, p?=?0.013), estimated resting twitch (?36?±?5?%, p?=?0.003) and voluntary activation (?17?±?9?%, p?=?0.021) versus pre-exercise. During last versus first maximal contraction in the fatiguing protocol lower voluntary torque (?40?±?4?%, p?=?0.003), higher MEP amplitudes (>+49?%, p?<?0.021) and longer silent periods (>+24?%, p?<?0.004) were recorded in both muscles. Decreased corticospinal excitability contributes significantly to the reduced maximal performance of fatigued lower limb muscles. During prolonged intermittent maximal dorsiflexions the performance of ankle muscles declines despite enhanced corticospinal excitability presumably due to deficient descending drive and/or spinal motoneuron responsiveness to the cortical drive.     

Changes in muscle function in response to 10 days of lower limb unloading in humans

Force-generating capacity and electromyographic (EMG) activity of the knee extensor muscles were studied before and after short-term (10 d) unilateral lower limb unloading and during 4 days of recovery. Ten healthy males used crutches to prevent one of their lower limbs from weight-bearing while maintaining joint mobility as well as daily ambulatory activities. Knee extensor torque and quadriceps rectified EMG during maximal voluntary isometric contraction (MVC) was measured repeatedly before and after the intervention. Also, EMG at a fixed submaximal level (100 Nm; 30–45% MVC) and maximal angular velocity (AV_max), during unresisted knee extension, were assessed. Maximum torque decreased (P<0.05) by 13±8% in response to unloading while maximum EMG activity did not change after unloading or during recovery (P=0.35). Submaximum EMG increased (P<0.05) by 25±16% after unloading. Maximum and submaximum torque/EMG ratios decreased (P<0.05) after unloading. AV_max decreased (P<0.05) by 9±8% after unloading. The post value, however, was not different from that of the weight-bearing limb. Torque, EMG and AV_max were recovered (P>0.05) after 4 days of resumed weight-bearing. The pronounced decrease and the rapid recovery in maximum torque appears not to be attributed to a change in muscle mass alone. Because the findings of unchanged maximum EMG and increased EMG at a submaximal force level suggest no change in neural drive, we propose that unspecific tissue factors in part impair muscle function in response to short-term loss of weight-bearing activity. Results also indicate that recovery in muscle function after short-term unloading seems to be completed in a time span shorter than the period of preceding inactivity.     

Effects of hyperoxia and hypoxia on dynamic and sustained static performance of the human quadriceps muscle

The influence of variations in inspired P_o2 on dynamic and static muscle performance of the left quadriceps muscle was studied. Eight subjects performed (1) 60 maximal consecutive dynamic contractions and (2) one sustained exhaustive static contraction at 27% of maximal voluntary contraction (MVC).Breathing mixtures containing 11%, 21% or 99% O₂, were administered. Peak torque as an average of the 60 knee exteasions was higher (p<0.01) during hyperoxia (mean ± SE= 104±4 Nm) than during normoxia (98±4 Nm), but did not differ significantly between hypoxia (95±5 Nm) and normoxia. Peak torque of individual extensions declined more rapidly during hypoxia than during normoxia, differing in the final 12 extensions by 11% from normoxic values. Static endurance time was reduced (p<0.02) during hypoxia (152±12 s) as compared to normoxia (189±13 s) and hyperoxia (169±11 s).No significant difference in endurance time was demonstrated between hyperoxia and normoxia. Thus, hypoxia impaired muscle performance in both dynamic and sustained static exercise, whereas acute hyperoxia improved dynamic but not static muscle performance. The results are interpreted in terms of differences in rate of intramuscular H⁺ accumulation.     

The repeated bout effect of eccentric exercise is not associated with changes in voluntary activation

The aim of this study was to compare the possible changes in muscle activation level between a first and second bout of damaging eccentric exercise performed at 2 weeks interval (i.e. repeated bout effect). To that purpose, ten physically active males took part in this study. The eccentric exercise consisted of 10 sets of 12 maximal voluntary contractions (MVC) produced by the knee extensors during movements performed at a constant speed of 160°s⁻¹. Changes in voluntary and electrically evoked torque in concentric and/or isometric conditions were assessed at the following time points: pre-exercise, and 2 min, 1 and 24 h after each eccentric exercise. At the same time points, voluntary activation was quantified by the superimposed electrical stimulation technique. Muscle soreness and plasma CK activity were measured within 48 h after the eccentric exercise. The results showed that the decrease in eccentric peak torque was linear throughout the exercise protocol. At the end of bouts 1 and 2, torque was significantly reduced by 27.7 ± 9.1 and 23.4 ± 11.2, respectively, with no difference between bouts (P > 0.05). At 24 h post-exercise, a lower reduction (P < 0.05) in MVC (17.8 ± 5.4%) and electrically evoked (16.7 ± 4.6%) isometric torque was observed for bout 2. In contrast, no statistical difference was found in the deficit in voluntary activation between the two bouts. In conclusion, our results indicate that the repeated bout effect of eccentric exercise appears to reduce muscle damage, but does not influence the level of voluntary activation.     

Changes in muscle contractile characteristics and jump height following 24 days of unilateral lower limb suspension

We measured changes in maximal voluntary and electrically evoked torque and rate of torque development because of limb unloading. We investigated whether these changes during single joint isometric muscle contractions were related to changes in jump performance involving dynamic muscle contractions and several joints. Six healthy male subjects (21 ± 1 years) underwent 3 weeks of unilateral lower limb suspension (ULLS) of the right limb. Plantar flexor and knee extensor maximal voluntary contraction (MVC) torque and maximal rate of torque development (MRTD), voluntary activation, and maximal triplet torque (thigh; 3 pulses at 300 Hz) were measured next to squat jump height before and after ULLS. MVC of plantar flexors and knee extensors (MVCke) and triplet torque decreased by 12% (P = 0.012), 21% (P = 0.001) and 11% (P = 0.016), respectively. Voluntary activation did not change (P = 0.192). Absolute MRTD during voluntary contractions decreased for plantar flexors (by 17%, P = 0.027) but not for knee extensors (P = 0.154). Absolute triplet MRTD decreased by 17% (P = 0.048). The reduction in MRTD disappeared following normalization to MVC. Jump height with the previously unloaded leg decreased significantly by 28%. No significant relationships were found between any muscle variable and jump height (r < 0.48), but decreases in torque were (triplet, r = 0.83, P = 0.04) or tended to be (MVCke r = 0.71, P = 0.11) related to decreases in jump height. Thus, reductions in isometric muscle torque following 3 weeks of limb unloading were significantly related to decreases in the more complex jump task, although torque in itself (without intervention) was not related to jump performance.     

Relative torque contribution of vastus medialis muscle at different knee angles

Aim: We investigated the relative contribution of the vastus medialis (VM) muscle to total isometric knee extension torque at 10°, 30°, 60° and 90° knee flexion. In the past a more prominent role of the VM muscle at more extended knee angles has been put forward. However, different components of the quadriceps muscle converge via a common distal tendon. We therefore hypothesized that the relative contribution of the VM to total knee extension torque would be similar across angles. Methods: At each knee angle the EMG isometric torque relations [20%, 25%, 30%, 35% maximal voluntary contraction (MVC)] of the rectus femoris (RF), vastus lateralis (VL) and VM muscle were established in 10 healthy male subjects; rectified surface EMG was normalized to M‐wave area. Subsequently, the VM was functionally eliminated by selective electrical surface stimulation with occluded blood flow. Results: There was no evidence for preferential activation of VM at any of the knee angles. Following VM elimination, total knee extension torque during maximal femoral nerve stimulation (three pulses at 300Hz) at 10°, 30°, 60° and 90°, respectively, decreased (P < 0.05) to (mean ± SD): 75.7 ± 12.2, 75.1 ± 9.3, 78.2 ± 7.2 and 76.0 ± 5.8% (P > 0.05 among knee angles). In addition, during voluntary contractions at 20% MVC the increases in torque output of RF and VL compensating for the loss of VM function were calculated from the increases in EMG and found to be similar (P > 0.05) at 10°, 30°, 60° and 90° values (%MVC), respectively, were: 9.1 ± 6.8, 7.5 ± 2.9, 5.9 ± 3.7 and 6.9 ± 3.4. Conclusion: The present findings support our hypothesis that the VM contributes similarly to total knee extension torque at different knee angles.     

Inter-individual variability in the adaptation of human muscle specific tension to progressive resistance training

Considerable variation exists between people in the muscle response to resistance training, but there are numerous ways muscle might adapt to overload that might explain this variable response. Therefore, the aim of this study was to quantify the range of responses concerning the training-induced change in maximum voluntary contraction (MVC) knee joint torque, quadriceps femoris (QF) maximum muscle force (F), physiological cross-sectional area (PCSA) and specific tension (F/PCSA). It was hypothesized that the variable change in QF specific tension between individuals would be less than that of MVC. Fifty-three untrained young men performed progressive leg-extension training three times a week for 9 weeks. F was determined from MVC torque, voluntary muscle activation level, antagonist muscle co-activation and patellar tendon moment arm. QF specific tension was established by dividing F by QF PCSA, which was calculated from the ratio of QF muscle volume to muscle fascicle length. MVC torque increased by 26 ± 11% (P < 0.0001; range −1 to 52%), while F increased by 22 ± 11% (P < 0.0001; range −1 to 44%). PCSA increased by 6 ± 4% (P < 0.001; range −3 to 18%) and specific tension increased by 17 ± 11% (P < 0.0001; range −5 to 39%). In conclusion, training-induced changes in F and PCSA varied substantially between individuals, giving rise to greater inter-individual variability in the specific tension response compared to that of MVC. Furthermore, it appears that the change in specific tension is responsible for the variable change in MVC.     

The genu effect on plantar flexor power

Triceps surae function can be modified by changes in knee joint angle through altering the effective contribution of the bi-articular gastrocnemeii. However, the impact on plantar flexor power from altering knee angle has not been studied systematically across a range of loads. Here, in 11 young men (25.7 ± 2.2 years), we determine the effect of knee angle on torque, velocity and power at loads ranging from 15 to 75 % maximal voluntary isometric contraction (MVC). Contractile properties were recorded with either the knee extended (170º) or flexed (90º). Despite similar voluntary activation (~97 %), peak twitch and MVC torques were 25 and 16 % lower in the flexed than extended knee (P < 0.05), respectively. Across all loads, subjects were 15–24 % less powerful with the knee flexed than extended (P < 0.05). In the flexed knee at relative loads ≤30 % MVC, impaired power was accompanied by 6–9 % slower shortening velocities than the extended knee. However, for the higher loads, limited torque production in the flexed knee was the key factor contributing to the generation of maximal power than for the extended position. This was supported by no change in velocity at higher loads (>30 % MVC) and a 15–22 % lower maximal rate of torque development across all loads. Hence, in a flexed knee position, which disadvantages the contribution of the gastrocnemeii, results in a left-downward shift in the torque–power relationship impairing maximal power production. Thus, the gastrocnemeii are not only a major contributor to plantar flexion torque, but also critical for modifying loaded shortening velocity and ultimately power production.     

Neuromuscular fatigue induced by whole-body vibration exercise

The aim of this study was to examine the magnitude and the origin of neuromuscular fatigue induced by half-squat static whole-body vibration (WBV) exercise, and to compare it to a non-WBV condition. Nine healthy volunteers completed two fatiguing protocols (WBV and non-WBV, randomly presented) consisting of five 1-min bouts of static half-squat exercise with a load corresponding to 50 % of their individual body mass. Neuromuscular fatigue of knee and ankle muscles was investigated before and immediately after each fatiguing protocol. The main outcomes were maximal voluntary contraction (MVC) torque, voluntary activation, and doublet peak torque. Knee extensor MVC torque decreased significantly (P < 0.01) and to the same extent after WBV (?23 %) and non-WBV (?25 %), while knee flexor, plantar flexor, and dorsiflexor MVC torque was not affected by the treatments. Voluntary activation of knee extensor and plantar flexor muscles was unaffected by the two fatiguing protocols. Doublet peak torque decreased significantly and to a similar extent following WBV and non-WBV exercise, for both knee extensors (?25 %; P < 0.01) and plantar flexors (?7 %; P < 0.05). WBV exercise with additional load did not accentuate fatigue and did not change its causative factors compared to non-WBV half-squat resistive exercise in recreationally active subjects.     

Can the electromyographic fatigue threshold be determined from superficial elbow flexor muscles during an isometric single-joint task?

The purpose of this study was to compare the electromyographic fatigue threshold (EMG_FT) values determined simultaneously from superficial elbow flexor muscles during an isometric single-joint task. Eight subjects performed isometric elbow flexions at randomly ordered percentages of maximal voluntary contraction (20, 30, 40, 50 and 60%). During these bouts, electromyographic (EMG) activity was measured in the anterior head of Deltoïd, lateral head of Triceps brachii, Brachioradialis and both short and long head of Biceps brachii. For each subject and each muscle, the EMG amplitude data were plotted as function of time for the five submaximal bouts. The slope coefficient of the EMG amplitude versus time linear relationships were plotted against force level. EMG_FT was determined as the y-intercept of this relationship and considered as valid only if the following criteria were met: (1) significant positive linear regression (P < 0.05) between force and slope coefficient, (2) an adjusted coefficient of determination for force versus slope coefficient relationship greater than 0.85, and (3) a standard error for the EMG_FT below 5% of maximal voluntary contraction. The EMG_FT could only be determined for one muscle (the long head of Biceps brachii) and only in three out of the eight subjects (mean value = 24.9 ± 1.1% of maximal voluntary contraction). The lack of EMG_FT in most of the subjects (5/8) could be explained by putative compensations between elbow muscles which were indirectly observed in some subjects. In this way, EMG_FT should be studied from a more simple movement i.e., ideally a movement implying mainly one muscle.     

Changes in force,cross-sectional area and neural activation during strength training and detraining of the human quadriceps

Summary Four male subjects aged 23–34 years were studied during 60 days of unilateral strength training and 40 days of detraining. Training was carried out four times a week and consisted of six series of ten maximal isokinetic knee extensions at an angular velocity of 2.09 rad·s⁻¹. At the start and at every 20th day of training and detraining, isometric maximal voluntary contraction (MVC), integrated electromyographic activity (iEMG) and quadriceps muscle cross-sectional area (CSA) assessed at seven fractions of femur length (L_f), by nuclear magnetic resonance imaging, were measured on both trained (T) and untrained (UT) legs. Isokinetic torques at 30° before full knee extension were measured before and at the end of training at: 0, 1.05, 2.09, 3.14, 4.19, 5.24 rad·s⁻¹. After 60 days T leg CSA had increased by 8.5%±1.4% (mean±SEM,n=4,p<0.001), iEMG by 42.4%±16.5% (p<0.01) and MVC by 20.8%±5.4% (p<0.01). Changes during detraining had a similar time course to those of training. No changes in UT leg CSA were observed while iEMG and MVC increased by 24.8%±10% (N.S.) and 8.7%±4.3% (N.S.), respectively. The increase in quadriceps muscle CSA was maximal at 2/10 L_f (12.0%±1.5%,p<0.01) and minimal, proximally to the knee, at 8/10 L_f (3.5%±1.2%, N.S.). Preferential hypertrophy of the vastus medialis and intermedius muscles compared to those of the rectus femoris and lateralis muscles was observed. Isoangular torque of T leg increased by 20.9%±5.4% (p<0.05), 23.8%±7.8% (p<0.05) and 22.5%±6.7% (p<0.05) at 0, 1.05 and 2.09 rad·s⁻¹ respectively; no significant change was observed at higher velocities and in the UT leg. Hypertrophy produced by strength training accounts for 40% of the increase in force while the remaining 60% seems to be attributable to an increased neural drive and possibly to changes in muscle architecture.     

Modulation of reflex responses in activated ankle dorsiflexors differs in healthy young and elderly subjects

During voluntary contractions, motor neurone activity is modulated by descending input and sensory feedback. Impaired excitatory afferent feedback with ageing may, therefore, alter motor control. This study investigated the age-related changes in afferent feedback through the recording of reflex responses during voluntary muscle activation. Short- and long-latency components of the stretch reflex and Hoffmann reflex (H-reflex) were recorded during voluntary contractions (10% of maximal voluntary contraction; MVC) of the ankle dorsiflexor muscles of young and elderly adults (≥70 years). Furthermore, the modulation of spinal reflex excitability was analyzed at different torque levels (10–50% MVC). The short-latency stretch reflex and the H-reflex areas were similar in the two age groups at 10% MVC whereas the area of the long-latency component of the stretch reflex augmented with ageing (P < 0.05). However, the area of the H-reflex increased linearly with the level of contraction up to 50% MVC in young adults, whereas it slightly increased to 30% MVC and plateaued thereafter in elderly adults. The absence of age-related changes in the short-latency stretch reflex and H-reflex areas suggests that the reflex circuitry and the sensitivity of the muscle spindles are not substantially affected by ageing. The modest increase in the H-reflex area with the contraction intensity in elderly adults, however, indicates that the modulation of afferent feedback is reduced with advancing age. This observation, associated with a greater long-latency stretch reflex, suggests that elderly adults rely more on central than peripheral mechanisms to regulate motor output of the dorsiflexor muscles.     

Low‐volume muscle endurance training prevents decrease in muscle oxidative and endurance function during 21‐day forearm immobilization

Aim: To examine the effects of low‐volume muscle endurance training on muscle oxidative capacity, endurance and strength of the forearm muscle during 21‐day forearm immobilization (IMM‐21d). Methods: The non‐dominant arm (n = 15) was immobilized for 21 days with a cast and assigned to an immobilization‐only group (Imm‐group; n = 7) or an immobilization with training group (Imm+Tr‐group; n = 8). Training comprised dynamic handgrip exercise at 30% of pre‐intervention maximal voluntary contraction (MVC) at 1 Hz until exhaustion, twice a week during the immobilization period. The duration of each exercise session was 51.7 ± 3.4 s (mean ± SE). Muscle oxidative capacity was evaluated by the time constant for phosphocreatine recovery (τ_offPCr) after a submaximal handgrip exercise using ³¹phosphorus‐magnetic resonance spectroscopy. An endurance test was performed at 30% of pre‐intervention MVC, at 1 Hz, until exhaustion. Results: τ _offPCr was significantly prolonged in the Imm‐group after 21 days (42.0 ± 2.8 and 64.2 ± 5.1 s, pre‐ and post‐intervention respectively; P < 0.01) but did not change for the Imm+Tr‐group (50.3 ± 3.0 and 48.8 ± 5.0 s, ns). Endurance decreased significantly for the Imm‐group (55.1 ± 5.1 and 44.7 ± 4.6 s, P < 0.05) but did not change for the Imm+Tr‐group (47.9 ± 3.0 and 51.7 ± 4.0 s, ns). MVC decreased similarly in both groups (P < 0.01). Conclusions: Twice‐weekly muscle endurance training sessions, each lasting approx. 50 s, effectively prevented a decrease in muscle oxidative capacity and endurance; however, there was no effect on MVC decline with IMM‐21d.     

Reduced plantarflexor specific torque in the elderly is associated with a lower activation capacity

Previous studies have reported a decrease in muscle torque per cross-sectional area in old age. This investigation aimed at determining the influence of agonists muscle activation and antagonists co-activation on the specific torque of the plantarflexors (PF) in recreationally active elderly males (EM) and, for comparison, in young men (YM). Twenty-one EM, aged 70–82 years, and 14 YM, aged 19–35 years, performed isometric maximum voluntary contractions (MVC). Activation was assessed by comparing the amplitude of interpolated supramaximal twitch doublets at MVC, with post-tetanic doublet peak torque. Co-activation of the tibialis anterior (TA) was evaluated as the ratio of TA-integrated EMG (IEMG) activity during PF MVC compared to TA IEMG during maximal voluntary dorsiflexion. Triceps surae muscle volume (VOL) was assessed using magnetic resonance imaging (MRI), and PF peak torque was normalised to VOL (PT/VOL) since the later approximates physiological cross-sectional area (CSA) more closely than anatomical CSA. Also, physical activity level, assessed by accelerometry, was significantly lower (21%) in the elderly males. In comparison to the YM group, a greater difference in PT (39%) than VOL (19%) was found in the EM group. PT/VOL and activation capacity were respectively lower by 25% and 21% in EM compared to YM, whereas co-activation was not significantly different. In EM PT/VOL correlated with activation (R²=0.31, P<0.01). In conclusion, a reduction in activation capacity may contribute significantly to the decline in specific torque in the plantar flexors of elderly males. The hypothesis is put forward that reduced physical activity is partialy responsible for the reduced activation capacity in the elderly.     

Resistance training increases in vivo quadriceps femoris muscle specific tension in young men

Aim: The present study investigated whether in vivo human quadriceps femoris (QF) muscle specific tension changed following strength training by systematically determining QF maximal force and physiological cross-sectional area (PCSA). Methods: Seventeen untrained men (20 ± 2 years) performed high-intensity leg-extension training three times a week for 9 weeks. Maximum tendon force (F_t) was calculated from maximum voluntary contraction (MVC) torque, corrected for agonist and antagonist muscle activation, and moment arm length (d_PT) before and after training. QF PCSA was calculated as the sum of the four component muscle volumes, each divided by its fascicle length. Dividing F_t by the sum of the component muscle PCSAs, each multiplied by the cosine of the respective fascicle pennation angle, provided QF specific tension. Results: MVC torque and QF activation increased by 31% (P < 0.01) and 3% (P < 0.05), respectively, but there was no change in antagonist co-activation or d_PT. Subsequently, F_t increased by 27% (P < 0.01). QF volume increased by 6% but fascicle length did not change in any of the component muscles, leading to a 6% increase in QF PCSA (P < 0.05). Fascicle pennation angle increased by 5% (P < 0.01) but only in the vastus lateralis muscle. Consequently, QF specific tension increased by 20% (P < 0.01). Conclusion: An increase in human muscle specific tension appears to be a real consequence of resistance training rather than being an artefact of measuring errors but the underlying cause of this phenomenon remains to be determined.     

Fatigue after short (100-m), medium (200-m) and long (400-m) treadmill sprints

The aim of this study was to compare the aetiology of neuromuscular fatigue following maximal sprints of different distances. It was hypothesized that increasing the distance would modify the type of peripheral and induce central fatigue. 11 subjects performed 100-, 200- and 400-m sprints on a motorized instrumented treadmill. Neuromuscular function, evaluated before (Pre), 30 s after (Post), and 5 and 30 min after the sprints (Post5 and Post30), consisted in determining maximal voluntary knee extensors torque (MVC), maximal voluntary activation of the knee extensors (%AL), maximal compound muscle action potential amplitude and duration on vastus lateralis, single twitch (Tw), and low- (Db10) and high-frequency torque. Compared with peak values, running speed decreased by 8%, (P < 0.01), 20% (P < 0.001) and 39% (P < 0.001) at the end of the 100-, 200- and 400-m sprints, respectively. MVC was not altered following 100 and 200 m, but decreased by 14% (P < 0.001) after the 400 m, was still depreciated Post5 (−11%, P < 0.01) and went back to initial values Post30. A decrease in %AL (−6.0%, P < 0.01) was observed Post5 for the 400 m. Tw, Db10 and low-to-high doublets ratio decreased Post-sprints and were not recovered Post30 after all sprints. Single maximal sprints of 100–400 m did not alter sarcolemmal excitability but induced progressive and substantial low-frequency fatigue and a slight reduction in neural drive with increasing sprint duration. Despite altered single or paired stimulations, MVC strength loss was detected only after the 400 m.     

Metabolically assessed muscle fibre recruitment in brief isometric contractions at different intensities

This study investigated the recruitment of type I, IIA and IIAX fibres after seven isometric contractions at 40, 70 and 100% maximal voluntary knee extension torque (MVC, 1 s on/1 s off). Biopsies of the vastus lateralis muscle were collected from seven subjects at rest and immediately post-exercise. Fibre fragments were dissected from the freeze-dried samples and characterized as type I, IIA and IIAX using mATPase staining. Phosphocreatine (PCr) and creatine (Cr) content were measured in the remaining part of characterized fibres. A decline in the ratio of PCr to Cr (PCr/Cr) was used as an indication of activation. The mean peak torques were, respectively, 39 (2), 72 (2) and 87 (6)% MVC. Cumulative distributions of type I and IIA fibres were significantly shifted to lower PCr/Cr ratios at all intensities (Kolmogorov-Smirnov test, P<0.05). The cumulative distribution of type IIAX fibres showed a significant leftward shift only at 87% MVC (P<0.05). A hierarchical order of fibre activation with increasing intensity of exercise was found, with some indication of rate coding for type I and IIA fibres. Evidence for activation of type IIAX fibres was only found at 87% MVC.