2种单组份自酸蚀黏结剂在牙V类洞边缘微渗漏的对比研究

目的单组份自酸蚀黏结剂是最新一代牙科黏结剂,操作简便。为研究其对牙本质和牙釉质的黏结性能,文中将S3BOND与ibond用于Ⅴ类洞树脂充填,对其边缘封闭性进行比较。方法 10颗新拔除的磨牙随机分为2组,在颊舌面分别制备Ⅴ类洞,龈壁位于釉牙本质界下方,A组用S3BOND自酸蚀黏结剂,B组用ibond自酸蚀黏结剂,2组均用维纳斯前后牙通用型纳米树脂充填,2%甲苯胺蓝染色,从充填体正中剖开牙齿,观察每个充填体龈壁和颌壁边缘微渗漏情况。结果2组龈壁存在不同程度微渗漏,差异有统计学意义(P<0.05),而颌壁间差异无统计学意义(P>0.05)。A组龈壁与颌壁染色的差异有统计学意义(P<0.05),B组龈壁与颌壁染色的差异无统计学意义(P>0.05)。结论自酸蚀黏结剂S3BOND在牙本质的边缘封闭性优于ibond,在牙釉质的边缘封闭性虽低于ibond,但差异不显著。     

自酸蚀黏结剂ibond用于后牙Ⅱ类洞边缘微渗漏的对比研究

目的 单组份自酸蚀黏结剂ibond是最新一代牙科黏结剂,操作简便,为研究其黏结性能,将ibond与另2种牙本质黏结剂用于后牙Ⅱ类洞树脂充填,对其边缘封闭性进行比较.方法 31个新鲜离体磨牙随机分为A、B、C 3组,每组10颗牙,另取1颗作阳性对照.在近、远、中邻面制备Ⅱ类洞,近中洞龈壁位于牙釉质,远中洞龈壁位于釉牙本质界下方,A组用Single Bond NT全酸蚀黏结剂,B组用可乐丽菲露SE BOND自酸蚀黏结剂,C组用ibond自酸蚀黏结剂黏结,3组均用可乐丽菲露AP-X树脂充填,2%甲苯胺蓝染色,从充填体正中剖开牙齿,观察充填体边缘微渗漏情况.结果 3组牙近中洞龈壁均无微渗漏,远中洞龈壁AB组存在不同程度微渗漏,但2组间无显著性差异,C组仅2例存在微渗漏,与AB组间有显著性差异(P＜0.05).结论 自酸蚀黏结剂ibond可较好地避免边缘微渗漏.     

牙面湿润方式对复合树脂微渗漏的影响

目的:比较不同牙面湿润方式对复合树脂微渗漏的影响.方法:选择24颗离体恒磨牙,在牙体颊舌侧分别制备48个V类洞,随机分为4组.用不同的湿润方式处理酸蚀后的牙面.用Adper Single Bond2黏接剂,z250复合树脂常规充填固化,经温差循环(5℃～55℃)100次,亚甲蓝染色,双目显微镜下观察牙体剖面充填体与洞壁间龈牙合肇染料渗透情况.结果:4组间<牙合>壁微渗漏无统计学差异(P>0.05),4组间龈壁微渗漏有显著统计学差异(P<0.05),75%乙醇再湿润组龈壁微渗漏明显低于吹干组(P<0.01).结论:以乙醇为溶剂的全酸蚀黏接系统,在湿黏接中的湿润方式以75%乙醇溶液棉球湿润洞壁微渗漏最少.     

2种黏结剂用于后牙Ⅱ类洞边缘微渗漏的对比研究

目的 后牙树脂充填后的边缘微渗漏问题是影响修复效果的关键因素之一,而黏结剂的边缘封闭性在其中起了关键的作用.文中对比了2种牙本质黏结剂用于后牙Ⅱ类洞树脂充填的边缘封闭性.方法 20个新鲜离体磨牙随机分为2组,在近远中邻面制备浅的Ⅱ类洞和深的Ⅱ类洞,A组用可乐丽菲露自酸蚀黏结剂,B组用Single bong NT全酸蚀黏结剂,2组均用可乐丽菲露前后牙通用型树脂充填,2%甲苯胺蓝染色,从充填体正中剖开牙齿,观察充填体边缘微渗漏情况.结果 2组牙浅洞洞壁均无微渗漏,深洞颌面侧壁无微渗漏,龈壁存在不同程度微渗漏,但2组无显著性差异(P>0.05).结论 自酸蚀黏结剂在牙颈部均存在牙本质黏结界面微渗漏,微渗漏程度较稳定,全酸蚀黏结剂虽可避免边缘微渗漏,但微渗漏程度差异较大,技术敏感性较高.     

充填器对树脂充填Ⅱ类洞后微渗漏的影响

目的 探讨CompothixoTM充填器、传统充填器、SonicFill声波手机3种充填器对树脂充填Ⅱ类洞效果的影响。方法 选取2020年10月至2021年2月山东第二医科大学附属医院（潍坊医学院附属医院）口腔科拔除的恒磨牙共40颗,随机分为A、B、C、D四组。在离体牙的近中、远中分别制备标准Ⅱ类洞,按各分组要求进行树脂充填,经温度循环、亚甲蓝染色后剖开,体视显微镜下观测牙体组织与修复体轴壁和龈壁的染料渗透深度并分级,计算微渗漏程度评分。结果 四组中均有样本发生微渗漏,经Mann-Whitney U检验,A组龈壁微渗漏评分高于另外三组（P<0.05）;B组与C组比较差异无统计学意义（P>0.05）;B组、C组均高于D组（P<0.05）。A组轴壁微渗漏评分高于D组（P<0.05）,其余组间比较差异无统计学意义（P>0.05）。结论 与传统充填器相比,CompothixoTM充填器有利于减少树脂与牙体组织间微渗漏;与使用SonicFill声波手机相比,使用CompothixoTM充填器并没有减少SonicFill超声树脂与牙体组织间微渗漏。     

自酸蚀粘接剂与纳米全酸蚀粘接剂对充填体边缘微渗漏的影响

目的:比较光固化树脂用自酸蚀粘接剂ONE-UP BOND F(俗称"变色龙")与3M纳米全酸蚀粘接剂Adper Single Bond2对充填体边缘的封闭情况。方法:取新鲜离体双尖牙40颗,在颌面各制备3mm×2mm×2mm箱状洞型,随机分成两组,A组使用ONE-UP BOND F粘接剂,B组使用3M ESPE Adper Single Bond2全酸蚀粘接剂,3Mp60后牙专用复合树脂充填,0.5%碱性品红溶液中染色24h后,将充填体近远中剖开,在立体显微镜下观察充填体边缘的染色情况。结果:两种粘接剂均有微渗漏现象发生,光固化树脂用自酸蚀粘接剂ONE-UP BOND F的边缘微渗漏情况略优于3M纳米全酸蚀粘接剂Adper Single Bond2,但差异无统计学意义(P>0.05)。结论:虽然两种粘接剂都不能消除充填体边缘的微渗漏,但ONE-UP BOND F自酸蚀粘接剂效果略好,而且操作简单,适合临床推广。     

氧化锆全瓷不同边缘位置对牙周组织的影响

目的观察氧化锆全瓷不同边缘位置对牙周组织的影响.方法 选择行氧化锆全瓷修复后6个月的临床病例30例,按冠边缘位置分为冠边缘平齐龈缘组(A组)15例,冠边缘位于龈下0.5~1 mm组(B组)15例,以A组和B组对侧或对颌健康同名牙做对照组.记录试验组和对照组牙龈指数(GI)、探诊深度(PD),并采用滤纸条法收集龈沟液,测定龈沟液量.结果 A组在临床牙周指标(GI、PD)与其对照组无统计学差异(P>0.05),而GCF量变化上有统计学差异(P<0.05).B组各项指标与其对照组比较均有统计学差异(P<0.05).A组与B组之间比较各指标也均有统计学差异(P<0.05).结论 不同牙冠边缘位置对牙周组织的影响不同.与对照组健康牙比较,冠边缘平龈边缘对牙周组织健康影响较小,龈下边缘较平龈边缘对牙周组织的影响大.     

牛磺酸对急性应激性骨骼肌损伤大鼠内质网应激作用的研究

目的 研究牛磺酸对离心运动大鼠骨骼肌中SOD、MAD、GRP78、GADD153的影响,以期解释牛磺酸通过减少内质网氧化应激保护骨骼肌损伤的机制。 方法 选取成年SD大鼠36只,分为空白对照1组(A组)、空白对照2组(B组)、1 d离心运动组(C组)、2 d离心运动组(D组)、1 d离心运动+牛磺酸组(E组)、2 d离心运动+牛磺酸组(F组)。培养后分别收集6组大鼠骨骼肌,采用黄嘌呤氧化酶法和硫代巴比妥酸法测定SOD、MAD含量,采用Western 印迹方法测定GRP78、GADD153表达,比较6组大鼠SOD、MAD、GRP78、GADD153的差异。 结果 A组、B组大鼠SOD含量高于C组、D组、E组、F组大鼠,与C组、D组大鼠比较差异具有统计学意义(均P<0.05),但与E组、F组大鼠比较差异无统计学意义(均P>0.05);E组、F组大鼠SOD含量高于C组、D组大鼠,差异无统计学意义(均P>0.05)。C组、D组大鼠MDA含量高于A组、B组、E组、F组大鼠(均P<0.05)。A组、B组、E组、F组大鼠SOD/MDA值均高于C组、D组大鼠(均P<0.05),E组、F组SOD/MDA值低于A组、B组(均P<0.05)。C组、D组大鼠GRP78、GADD153表达量高于A组、B组(均P<0.05),但低于E组、F组大鼠(均P<0.05);E组、F组大鼠GRP78、GADD153表达量高于A组、B组(均P<0.05)。 结论 牛磺酸可能是通过提高内质网的抗氧化应激能力,从而维持骨骼肌抗氧化能力,加快骨骼肌中氧自由基的清除,减少剧烈运动后机体骨骼肌的氧化应激损伤,有利于骨骼肌功能的恢复。      

人离体牙树脂修复充填前、后漂白对边缘微渗漏的影响

目的：评价树脂充填前、后漂白对树脂修复体边缘微渗漏的影响。方法收集2015年5月至2016年1月在深圳市福田区中医院口腔科60颗离体上颌中切牙(重度牙周病患者无法保留的患牙),分别行根管治疗后,按患者就诊先后顺序随机分成3组(n=20)：A组直接树脂充填;B组牙漂白术后行树脂修复术;C组树脂充填后行牙漂白术;模拟口腔内环境处理后,在体视显微镜下观测牙漂白术对树脂修复体微渗漏的影响。结果 A组修复体边缘微渗漏率为35%,B组为90%,C组为95%,A组修复体边缘微渗漏率低于B组和C组,差异有统计学意义(P>0.05), B与C组间比较差异无统计学意义(P>0.05)。结论无论树脂充填前或充填后漂白,漂白术均会使复合树脂充填体的边缘微渗漏程度增加。     

E-tracking检测川崎病患儿动脉弹性功能的临床应用

目的：运用E-tracking检测川崎病患儿颈动脉弹性,了解其功能变化情况。方法将川崎病患儿按病程的随访时间进行分组,分为A组（1周内急性病）（n=34）、B组（病程4周）（n=34）、C组（病程8周）（n=34）、D组（病程24周）（n=29）、E组（病程48周）（n=25）;对照组F组为健康儿童（n=27）。运用E-tracking技术检测各组儿童颈动脉,测量其压力应变弹性系数(Ep)、硬化度(β)和顺应性(AC),将检测数据进行比较分析。结果弹性系数Ep(kPa)：A与B、C比较,B与C、D比较,C与D比较,D与E比较,E与F比较,差异均无统计学意义;A与D、E、F比较,B与E、F比较,C与E、F比较,D与F比较,差异有统计学意义（P＜0.05）。僵硬度β：A与B、C、D比较,B与C比较,C与D比较,D与E比较,差异均无统计学意义。A与E、F比较,B与D、E、F比较,C与E、F比较,D与F比较,E与F比较,差异有统计学意义（P＜0.05）。顺应性AC：A与B、C比较,B与C、D比较,C与D、E比较,D与E比较,E与F比较,差异无统计学意义;A与D、E、F比较,B与E、F比较,C与F比较,D与F比较,差异有统计学意义（P＜0.05）。结论 E-tracking技术能检测川崎病患儿颈动脉弹性及内皮功能损伤情况;川崎病急性期及恢复期6个月以内均存在颈动脉内皮功能的改变,1年后其内皮功能基本恢复正常。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.