血浆内皮素与肾小球疾病发病关系的探讨

用放射免疫法测定22例正常儿童血浆内皮素（ET）含量（84．13±26．16pg／ml）±s，健康成人组26例（88．43±26．27pg／ml）；急性肾炎急性或9例（106．55±21．11pg／ml），急性肾炎恢复期15例（77．63±34．11pg／ml），单纯性肾病10例（140．03±51．80pg／ml），成人慢性肾炎组11例（223．32±68．09pg／ml）。     

东莞市学龄前儿童维生素A缺乏现状及维生素A对血清铁蛋白及红细胞参数的影响

目的 了解东莞市学龄前儿童维生素A缺乏现状,探讨维生素A对血清铁蛋白、红细胞及网织红细胞参数的影响。方法 于2015年4月至2016年12月通过整群抽样方法,选取东莞市无现患疾病的学龄前儿童（3~6岁）2 085例,对所选儿童进行血常规、网织红细胞计数、血清铁蛋白、血红蛋白电泳及维生素A浓度检测。分析年龄、性别与维生素A浓度及血清铁蛋白浓度的关系,维生素A浓度对血清铁蛋白、红细胞及网织红细胞参数的影响以及维生素A缺乏加重储存铁减少对红细胞参数的影响。结果 储存铁减少的儿童占比为6.71%（140/2 085）;维生素A缺乏儿童占比为32.52%（678/2 085）,其中亚临床缺乏占维生素A缺乏总人数的95.4%（647/678）,临床缺乏占维生素A缺乏总人数的4.6%（31/678）。不同性别组儿童维生素A浓度比较差异无统计学意义（P > 0.05）,但女性儿童血清铁蛋白浓度高于男性（P < 0.05）。维生素A临床缺乏组儿童血清铁蛋白浓度高于亚临床缺乏组和正常组（P < 0.05）。维生素A缺乏时,储存铁减少组平均红细胞体积和平均红细胞血红蛋白含量较储存铁正常组降低（P < 0.05）。维生素A缺乏组血红蛋白浓度、平均红细胞血红蛋白浓度、红细胞计数、红细胞压积、网织红细胞绝对值、网织红细胞百分比、网织红细胞血红蛋白含量均低于维生素A正常组,而平均红细胞体积高于维生素A正常组（P < 0.05）。结论 东莞市学龄前儿童维生素A缺乏状况仍较严重;维生素A缺乏可对血清铁蛋白、红细胞以及网织红细胞参数产生影响。     

肺炎支原体肺炎患儿血清神经生长因子和白细胞介素-15的水平变化及其临床意义

目的探讨肺炎支原体肺炎（mycoplasma pneumoniae pneumonia, MPP）患儿血清中神经生长因子（nerve growth factor,NGF）、白细胞介素（interleukin,IL）-15的水平变化及其临床意义。方法采用酶联免疫吸附法对65例MPP患儿（重症组25例,轻症组40例）和50例健康儿童（对照组）的血清NGF和IL-15水平进行测定。结果检测血清NGF、IL-15水平,MPP组急性期分别为（157．62±33．45）pg／ml和（242．51±60．04）pg／ml,恢复期分别为（99．58±21．29）pg／ml和（145．90±50．25）pg／ml,均较对照组明显升高[（29．86±11．74）pg／ml,（108．86±21．14）pg／ml],且急性期NGF、IL-15水平显著高于恢复期,差异有统计学意义（P〈0．05）。在MPP急性期,重症组血清NGF、IL-15水平显著高于轻症组[（204．38±27．52）pg／mlvs（128．39±22．07）pg／ml,（288．58±55．33）pg／mlvs（213．71±42．69）pg／ml],差异有统计学意义（P〈0．05）;在MPP恢复期,重症组血清NGF、IL-15水平高于轻症组,差异无统计学意义（P〉0．05）。结论肺炎支原体感染后,患儿血清中NGF、IL-15水平明显升高,随着病情的缓解而降低,提示NGF、IL-15参与肺炎支原体感染的发病过程。     

血红蛋白和红细胞原卟啉与海拔关系的初步探讨

研究目的探讨血红蛋白（Hb）和红细胞原卟啉（EP）水平与海拔高度的关系研究方法青海省贵南县及玛多县海拔高度分别为3200米和4300米。选该两县7岁～14岁在校健康中小学学生各122人，用滤纸片高铁氰化法检测血红蛋白，用滤纸片荧光分光光度计法检测红细胞原卟啉，用t检验和配对t检验进行统计学处理。结果贵南县和玛多县7岁～14岁建康儿童Hb分别为133．6±10．1，152．8±14．0g／L（t＝12．31，P＜0．001），EP分别为320．7±114．9，347．8±123．6μg／L（t＝1．77，P＞0．05），EP／Hb分别为2．4±0．9，2．3±0．8（t＝1．12，P＞0．05）。配对t检验显示，Hb、EP、EP／Hb男女之间无显著性差异（P＞0．05）结论随海拔高度增加，7岁～14岁儿童Hb及EP水平显著升高，无性别差异。     

上海地区儿童血清胃泌素水平流行病学调查

为了解儿童血清胃泌素 (GS)水平及有关影响因素 ,用放射免疫分析法测定296名6～12岁学生血清GS ,按血清GS测定值分为A、B、C3组 ,A组GS>90pg/ml,B组60～90pg/ml,C组<60pg/ml,同时由学生家长填写问卷调查表。结果 :296名学生空腹血清GS均值为 (70.95±18.62)pg/ml,男 (68.12±20.81)pg/ml,女(72.35±19.24)pg/ml,男女间差异无显著性。三组儿童的个人饮食习惯、家庭进食方法、胃病家庭史和家庭规模等差异均无显著性。血清GS水平与儿童是否服用过抗生素、父(或母 )文化程度以及儿童是否存在消化道症状等也无显著相关性。提示儿童血清GS水平是综合因素影响的结果     

毛细支气管炎患儿外周血白细胞介素-12的意义

目的探讨白细胞介素（interleukin,IL）-12在毛细支气管炎发病过程中的意义。方法选取59例2岁以下毛细支气管炎患儿,分为毛支Ⅰ组（n=28）和毛支Ⅱ组（n=31）,其中毛支Ⅰ组为具有特应质高危因素的患儿,毛支Ⅱ组为无特应质高危因素的患儿。同期住院的同年龄段支气管肺炎患儿36例和患有疝气、肾结石等非感染性疾病术前患儿31例分别作为肺炎对照组和正常对照组,分别检测4组患儿外周血IL－12的水平。结果毛支Ⅰ组患儿外周血IL-12为（34．72±7．96）pg／ml;毛支Ⅱ组患儿外周血IL-12为（55．30±6．72）pg／ml;肺炎对照组患儿及正常对照组患儿外周血IL-12分别为（56．79±10．36）pg／ml、（61．23±11．51）pg／ml,其中毛支Ⅰ组与毛支Ⅱ组相比,外周血IL-12水平降低,差异有统计学意义（P〈0．05）;毛支Ⅰ组与肺炎对照组、正常对照组相比,外周血IL-12水平显著降低,差异有统计学意义（P〈0．05）;毛支Ⅱ组与肺炎对照组、正常对照组相比,外周血IL-12水平降低,差异有统计学意义（P〈0．05）;肺炎对照组与正常对照组相比,外周血IL-12水平差异无统计学意义（P〉0．05）。结论血清IL-12降低是毛细支气管炎发病的重要因素,具有特应质高危因素的毛细支气管炎患儿IL-12降低更为明显。     

0~7岁儿童25羟维生素D和骨密度的关系研究

目的 评价 0~7 岁儿童维生素 D（VitD）营养状况及与骨密度的关系。方法 选择生长发育门诊因“生长发育缓慢、夜惊、多汗、烦躁不安”等就诊的儿童 6 838 人,采用化学发光法法测定血清 25 羟VitD [25-（OH）D] 水平,并同时应用定量超声仪进行骨密度检测。结果 研究对象血清 25-（OH）D 的水平为34±14 ng/mL,定量超声骨密度 Z 值为 -0.49±0.54。随着年龄增长,25-（OH）D 和骨密度水平逐渐降低,且 VitD缺乏、不足及骨密度不足的检出率逐渐增加（PPP结论 学龄前期和学龄期儿童 VitD 不足和缺乏的现象较婴幼儿更严重。在一定范围内 VitD 水平和骨密度可能有关。     

持续去甲肾上腺素输注对早期脓毒症大鼠肾脏的保护作用及其机制研究

目的探讨持续去甲肾上腺素输注对早期脓毒症大鼠肾脏是否存在保护作用及其可能机制。方法30只SPF级健康雄性SD大鼠采用随机数字表法随机分为5组（6只／组）。对照组：腹腔注射生理盐水,并开始持续输注生理盐水1ml／h。脂多糖（lipopolysaccharide,LPS）组和低剂量、中剂量和高剂量去甲肾上腺素组：腹腔注射LPS10mg／kg,LPS组持续输注生理盐水1ml／h,低、中、高剂量组分别持续输注0．06、0．30、0．60μg／（kg·min）去甲肾上腺素溶液1ml／h,均持续输注24h处死大鼠。检测2、6h大鼠血清炎症因子肿瘤坏死因子（tumor necrosis factor,TNF）-α、白细胞介素（interleukin,IL）-1β、IL-6及IL-10水平,24h大鼠血清C-反应蛋白（CRP）、肌酐（Cr）和尿素氮（BUN）水平,膜电位和氧化应激相关指标,电镜观察肾脏线粒体肿胀程度,显微镜观察肾脏病理变化。结果与对照组比较,2hLPS组TNF—α浓度为（2203．3±1028．7）pg／ml、IL-1β（2214．5±457．0）pg／ml、IL-6（7784．7±248．2）pg／ml及IL-10（1076．1±368．4）pg／ml,均明显升高（P〈0．05）;24hCRP（0．35±0．24）mg／L,Cr（30．8±11．5）μmol／L,BUN（7．7±1．8）mmol／L,一氧化氮（1057．4±172．9）μmol／gprot,均明显升高（P〈0．01）,肾脏线粒体膜电位0．0464±0．0185,明显下降（P〈0．01）。与LPS组比较,2h低剂量组TNF-d浓度为（506．8±301．7）pg／ml、IL-1β（415．6±178．0）pg／ml及IL-α（381．7±171．0）pg／ml,均明显下降（P〈0．05）,24hBUN下降为（5．2±1．4）mmol／L（P〈0．05）,线粒体膜电位上升为0．3474±0．1526（P〈0．05）;2h中剂量组TNF-α浓度为（323．9±227．9）pg／ml、IL-1β（700．0±246．2）pg／ml,IL-10（282．6±134．4）pg／ml,均明显下降（P〈0．05）,24hCRP下降为（0．17±0．08）mg／L（P〈0．05）,线粒体膜电位上升为0．3775±0．1437（P〈0．05）;高剂量组2hTNF-α浓度为（378．7±89．8）pg／ml、IL-1β（945．7±264．4）pg／ml,明显下降（P〈0．05）,24hCRP（0．19±0．12）mr／L、Cr（23．2±3．4）μmol/L均明显下降（P〈0．05）。电镜观察显示LPS组线粒体包膜模糊及基质空泡化、凝固,显微镜观察显示LPS组肾组织间隙水肿,单核-巨噬细胞浸润,肾小球皱缩,肾小管上皮细胞肿胀、空泡变性等,中剂量组病理损伤明显减轻。结论持续去甲肾上腺素输注对早期脓毒症大鼠肾脏有一定的保护作用,其机制与降低炎症因子风暴的水平、减轻氧化应激损害、改善线粒体功能相关。     

1型糖尿病患儿血清白细胞介素—6水平的变化及意义

为探讨白细胞介素-6(IL-6)在1型糖尿病发病中的作用,采用ELISA法测定26例1型糖尿病患儿、20例健康儿童血清IL-6水平,糖尿病组还同时测定空腹血糖、糖化血红蛋白(HbA_(1c))、血清胰岛素、C肽。结果显示1型糖尿病患儿血清IL-6水平(12.4pg/ml±4.7pg/ml)明显高于正常对照组(4.1pg/ml±2.3pg/ml),t=7.24,P<0.01;糖尿病患儿中病情控制不良组(HbA_(1c)>10.0％)血清IL-6显著高于病情控制良好组(HbA_(1c)<10.0％),20.8pg/ml±4.8pg/ml对6.4pg/ml±2.7pg/ml,t=9.62,P<0.01;糖尿病组血清IL-6水平与病程、空腹血糖、血清胰岛素、C肽均无相关性(P>0.05)。提示IL-6在儿童1型糖尿病的发病中起一定作用。     

基于儿童血清多种维生素水平构建儿童抽动障碍预测模型及其临床价值分析

目的　探讨血清中多种维生素浓度与抽动障碍（TD）相关性,从而为临床实践提供更系统全面的循证学依据。方法　回顾2018年7月至2021年6月就诊首都儿科研究所附属儿童医院神经内科的1089例TD患儿临床资料;对照同期医院保健科健康体检的753例儿童相关资料,分析两组儿童血清多种维生素水平。随机按7：3的比例分为训练集和验证集,采用R软件中的caret包,在训练集中比较维生素A（VA）、维生素B1（VB1）、维生素B2（VB2）、维生素B6（VB6）、维生素B9（VB9）、维生素B12（VB12）、维生素C（VC）、维生素D（VD）、维生素E（VE）的水平并构建预测模型。并对其进行可靠性验证。结果　训练集儿童Logistic回归风险模型提示：儿童血清VA、VB1、VB2、VB6、VB12、VD的水平、男性和年龄是影响TD发生的独立危险因素。结合Logistic模型,绘制列线图。可视化列线图提示：年龄在4～10岁、男性、以及VA、VB1、VB2、VB6、VD不足或缺乏儿童的赋分高。列线图训练集一致性指数C-index为0.761（95% CI：0.708～ 0.813）,证实列线图模型达到中上等区分度,有临床价值。训练集受试者工作特征曲线（ROC）结果显示曲线下面积（AUC）=0.737,校准曲线和决策曲线分析法（DCA）分析显示模型的可靠性较好,验证集中的ROC结果显示AUC=0.726,表明该模型具有一定的临床参考价值。结论　儿童血清VA、VB1、VB2、VB6、VB12、VD的水平、男性和年龄是影响TD发生的独立危险因素。由列线图可见4~10岁的男性儿童不足或缺乏VA、VB1、VB2、VB6和VD更易出现抽动障碍,因此,抽动障碍患儿血清VA、VB1、VB2、VB6和VD的水平与抽动障碍之间存在显著的相关性,对于抽动障碍儿童应常规评估维生素营养状况,为TD的治疗奠定基础。     

中国4城市2～7岁分析人群维生素B12营养状况流行病学调查

目的了解4城市2～7岁分析人群维生素B12的营养状况。方法2007年5～9月和2008年5～10月,对2 002名2～7岁分析人群进行体格检查、膳食调查、血常规检查及血清维生素B12水平测定,其中重庆市485名、北京市513名、珠海市503名、武汉市501名。结果①2 002名2～7岁分析人群平均维生素B12水平 （ng·L-1）为775±312,市区（835±271）高于郊区（720±338）,P＜0.001;其中重庆市为756±220、北京市为840±414、珠海市为772±245、武汉市为736±317,地区间差异有统计学意义,P＜0.001。②2～7岁分析人群维生素B12水平性别差异不显著,P＞0.05;2～5岁组维生素B12水平随年龄增长呈降低趋势,r=-0.146,P＜0.001;5岁～组和<7岁组维生素B12水平相近。③4城市分析人群维生素B12减少（＜300）检出率为4.7%。其中维生素B12缺乏（＜200）检出率为1.5%,边缘性缺乏（200～300）检出率为3.2%。重庆市、北京市、珠海市和武汉市分析人群血清维生素B12减少检出率分别为2.1%、3.9%、3.2%和9.6%。④分析人群维生素B12水平主要受膳食维生素B12摄入量的影响,P＜0.001。分析人群平均膳食维生素B12摄入量（μg·d-1）市区（2.4±2.0）高于郊区（2.0±1.4 ）,P＜0.05;重庆市、北市京、珠海市和武汉市分析人群平均膳食维生素B12摄入量分别为1.9±1.3、2.8±1.6、2.6±1.8和1.6±1.2,差异有显著统计学意义,P＜0.001。⑤分析人群维生素B12水平与Hb水平呈低度相关,P＜0.001。结论4城市分析人群存在维生素B12减少,以边缘性缺乏为主。儿童维生素B12营养状况受膳食维生素B12摄入量的影响,分析人群血清维生素B12水平何时达到成人水平有待研究。     

儿童急性白血病血清白细胞介素24的测定及意义

目的观察急性白血病患儿血清白细胞介素24(IL-24)的水平及意义。方法确诊未治的儿童急性淋巴细胞白血病(ALL)、儿童急性髓细胞白血病(AML)、非白血病患儿及正常儿童(正常对照组)各20例,采用ELISA法测定其血清IL-24的水平并进行比较。结果两组白血病患儿血清IL-24浓度[ALL组:(28.25±2.6)ng/L;AML组:(26.32±3.2)ng/L]均较正常对照组[(113.15±3.7)ng/L]和非白血病患儿[(105.82±4.2)ng/L]明显降低(P值均0.05);但ALL与AML组之间和非白血病组与正常对照组之间差异无统计学意义(P值均0.05)。结论 ALL及AML患儿血清IL-24浓度明显下降。     

Treatment of iron deficiency anemia with ferrosulfate serin komplex (author's transl)

The results of treatment of iron deficiency anemia in 217 children and infants (aged 8 months to 12 years) with ferrosulfate serin complex are demonstrated: 208 children with simple iron deficiency anemia with hemoglobin 9.1 g/100 ml before treatment had in the first 4 weeks a daily increase of 0.082 g/10000 ml hemoglobin; in 3 children with severe anemia of chronic blood loss the daily increase was 0.21 mg/100 ml and in 6 children with hypoxic polycythemia with congenital heart disease and so called latent iron deficiency anemia the increse was 0.16 g/100 ml. Through the better absorption of this serin-ferrosulfate complex the required dosis is only 3 mg/kg/d. The benefit of this small dosis is the low rate of side effects.     

Plasma ghrelin levels in various stages of development of iron deficiency anemia

OBJECTIVES: Ghrelin stimulates food intake and induces metabolic changes leading to an increase in body weight and body fat mass. Iron-deficiency anemia (IDA) is the most frequently seen cause of nutritional anemia, that is a type of starvation. There is no available study related to levels of ghrelin in IDA. The aim of this study is to show an association with ghrelin levels and iron deficiency and to demonstrate whether changes seen in iron deficiency (ID) are explained by ghrelin, as opposed to whether ghrelin levels correlate with ID. MATERIALS AND METHODS: The study group was consisted of children who were admitted in the outpatient clinic of pediatrics. Control group (C) was defined as cases with normal hemoglobin (Hb), serum iron (SI), transferrin saturation (TS), and ferritin (F) (>12 ng/mL) values; group hypoferritinemia (IDec) Hb: N, SI: N, TS: N, F<12 ng/mL; group iron deficiency (IDef), Hb: N, SI: decreased, TS相似文献   

Measurement of reticulocyte hemoglobin content to diagnose iron deficiency in Saudi children

Iron deficiency and iron deficiency anemia are common conditions in children, especially in developing countries. It is often difficult for the pediatrician to know which indices should be used in the diagnosis of these conditions in children. Reticulocyte hemoglobin (Hb) content (CHr) has been shown to be an accurate indicator of anemia, however whether its use suits the situation in developing countries or not is unclear. The aim of this study was to evaluate the value and effectiveness of using CHr as a method to diagnose iron deficiency and iron deficiency anemia in Saudi children. The samples for the study were collected from 305 children suspected to have anemia. Complete blood count, transferrin saturation (Tfsat), ferritin, circulating transferrin receptor (TfR) and CHr were measured. Three groups were defined, iron deficiency (Tfsat <20%, Hb >11 g/dL; n=120), iron deficiency anemia (Tfsat <20%, Hb <11 g/dL; (n=73) and controls (Tfsat >20%; n=112). The anemic group had significantly lower macrocytic anemia (MCV), mean corpuscular hemoglobin (MCH) and CHr. All of the variables in the anemia group were significantly lower than those of the control group except for the ferritin level. Compared to the control group, the iron deficiency group also showed significantly lower values except for transferrin receptor and the ferritin levels. CHr levels of <26 pg correlated well with anemic states. CHr together with a complete blood count may provide an alternative to the traditional hematologic or biochemical panel for the diagnosis of iron deficiency and iron deficiency anemia in young children and is cost-effective in developing countries. A CHr cut-off level of 26 pg is considered to be a reasonable indicator of anemic states.     

Nutritional vitamin B12 deficiency in hospitalized young children

The authors sought to determine prevalence, social, economic, and dietary patterns of young children (n = 20) identified as having vitamin B₁₂ deficiency anemia after admission to their hospital in the last 3 years. The diagnosis of vitamin B₁₂ deficiency was based on symptoms and clinical findings, findings on peripheral blood films and bone marrow aspirates, and serum levels of vitamin B₁₂. The children had been exclusively breast-fed without any animal food supplementation. Serum vitamin B₁₂ levels were also measured in the sera of mothers and found to be low. The authors concluded that vitamin B₁₂ deficiency might be an important health problem among children of mothers who do not consume animal foods adequately.     

Beneficial Effect of Iron Pot Cooking on Iron Status

Objectives

To develop iron rich snacks using locally available iron rich foods and analyze their iron content when cooked in iron pots. Further, the efficacy of the developed snacks, cooked in iron pots was examined on the hemoglobin status of pre-school children through a three month randomized trial.

Methods

Four iron rich snacks (mean iron content 2.1mg/serving) were cooked in iron pots and 27 preschool children (mean age 2.9?±?0.9 y, 12 boys) were supplemented with the snacks for 4 mo. Anthropometry and dietary intake data were collected. Hemoglobin, serum iron and transferrin saturation were assessed.

Results

An increase of 16.2 % in the iron content was found in the snacks cooked in iron pots than cooked in Teflon coated non-stick pots. After 4 mo of supplementation, a significant increase of 7.9 % was seen in the hemoglobin of the children.

Conclusion

This pilot study demonstrated that iron rich recipes cooked in iron pot have a beneficial effect on iron status of children. Therefore, such food based strategies have the potential to alleviate iron deficiency anemia not only in children but also in other vulnerable sections of society like in pregnant women.     

Anemia and iron deficiency among schoolchildren in the Aral Sea region,Kazakhstan

The objectives of this study were to estimate the prevalence of anemia and iron deficiency among schoolchildren in the Aral Sea region of Kazakhstan and to determine the various factors associated with anemia in this population. We conducted a cross-sectional study of randomly selected schoolchildren. Blood samples were collected for measuring hemoglobin (Hb), serum ferritin (SF), total iron binding capacity (TIBC), and other hematological indices, and subjects were screened for anemia and iron deficiency. Associations between Hb concentration and SF, TIBC, anthropometric, and socioeconomic data were evaluated using regression analysis. The prevalence of anemia was 49.8 per cent although levels were mostly mild. Twenty-two per cent of the children were iron depleted (SF < 12 microg/l). Of the anemic children, 32.4 per cent were found to have iron deficiency anemia (anemia with SF < 12 microg/l). There were significant positive correlations between the levels of Hb and SF, but a negative correlation with serum TIBC. Age, mean corpuscular volume (MCV) and SF were found to be significantly related to Hb by stepwise multiple regression analysis. Multiple logistic regression analysis revealed that anemia was independently related to living district, education of father, and child's age. The results suggest that iron deficiency is an important determinant of anemia in this population; however, whole anemia cannot be solely explained by iron deficiency. Further studies are needed for consideration of micronutrients status, parasite infestation, hereditary disorders, and exposure to environmental pollutants.     

新生儿缺氧缺血性脑病血清IL-8水平变化研究

目的　白细胞介素 8(IL 8)为缺血 /再灌注时炎症细胞的释放产物 ,并可引起细胞损伤。该研究旨在探讨IL 8是否参与新生儿缺氧缺血性脑病 (HIE)脑缺血 /再灌注损伤。方法　采用双抗体夹心ELISA法检测5 0例HIE患儿 (HIE组 ,其中轻度HIE 1 8例 ,中度HIE 1 7例 ,重度HIE 1 5例 ;合并感染者 2 9例 ,未合并感染者 2 1例 )、30例正常新生儿 (正常对照组 )及 2 0例患感染性疾病无HIE患儿 (感染组 )血清IL 8水平 ,HIE患儿经治疗后复查血清IL 8。结果　HIE组血清IL 8水平高于对照组 (2 1 .5 2± 9.5 9pg/mlvs 1 4 .4 3± 4 .84 pg/ml) ,差异有显著性 (P <0 .0 1 ) ;重度HIE患儿血清IL 8水平高于轻度HIE组 (2 6 .0 7± 1 3.83pg/mlvs 1 7.5 6± 6 .5 2pg/ml) ,差异有显著性 (P <0 .0 5 ) ,与中度HIE组比较 (2 1 .71± 5 .6 5 pg/ml) ,差异无统计学意义 (P >0 .0 5 ) ;HIE患儿治疗后IL 8水平较治疗前下降 (1 4 .5 3± 4 .87pg/mlvs 2 2 .6 0± 7.0 6 pg/ml) ,差异有显著性 (P <0 .0 1 ) ;有感染合并症HIE患儿血清IL 8水平高于无感染合并症患儿及感染组患儿依次为 2 3.79± 1 1 .0 4pg/ml,1 8.38± 6 .0 7pg/ml,1 8.2 2± 8.0 1 pg/ml,差异有显著性 (P <0 .0 5 )。结论　新生儿HIE时血清IL 8升高 ,病情越重升高越显著     

2,3-DIPHOSPHOGLYCERATE LEVELS IN CHILDREN WITH IRON DEFICIENCY ANEMIA AND ACUTE LEUKEMIA

Abstract. Red cell organic phosphates and especially 2,3-diphosphoglycerate (2,3-DPG), lowers the oxygen affinity of hemoglobin (Hb) and shifts the oxygen dissociation curve to the right. Because of the importance of 2,3-DPG (as regulator of the oxygen affinity of Hb), determinations were carried out on: 45 normal children, 7 children with iron deficiency anemia and 35 children with acute lymphoblastic leukemia. In normal children with Hb of 12.69±1.60 g/100 ml, 2,3-DPG was 14.90±0.68 μmoles/g Hb. In children with iron deficiency anemia (Hb 7.94±1.20 g/100 ml), 2,3-DPG was 20.87±3.11 μmoles/g Hb. 2,3-DPG was normal (14.11±0.88 μmoles/g Hb), in 16 patients with acute lymphoblastic leukemia who had never relapsed, while in 19 patients with a history of one or more relapses, 2,3-DPG levels were increased (22.05 ±2.75 μmoles/g Hb). No good explanation may be offered for the high 2,3-DPG levels in these leukemic children.