二硫代氨基吡咯烷预先给药对内毒素诱发大鼠心肌损伤的影响

目的 评价二硫代氨基吡咯烷预先给药对LPS诱发大鼠心肌损伤的影响.方法 成年Wistar大鼠72只,体重200～250 g,雌雄各半,随机分为3组(n=24):对照组(C组)、LPS组和二硫代氨基吡咯烷组(PDTC组).LPS组腹腔注射LPS 8 mg/kg;PDTC组尾静脉注射二硫代氨基吡咯烷120 mg/kg,30 min后腹腔注射LPS 8 mg/kg;C组注射等量生理盐水.分别于注射LPS后1、3/6、12 h时,腹腔注射2%戊巴比妥钠40 mg/kg麻醉大鼠,下腔静脉取血样后处死,开胸取心脏,采用ELISA法测定血清肌钙蛋白T(cTnT)浓度,免疫组化法检测心肌组织Toll样受体4(TLR4)的表达和NF-κB p65的活性;光镜下观察心肌组织病理学结果.结果 与C组比较,LPS组和PDTC组各时点血清cTnT浓度升高,心肌组织TLR4表达上调,NF-κB p65活性升高(P＜0.01);与LPS组比较,PDTC组各时点血清cTnT浓度下降,心肌组织NF-κB p65活性降低,注射LPS后6、12 h时心肌组织TLR4表达下调(P＜0.01),病理学损伤减轻.结论 二硫代氨基吡咯烷可减轻LPS诱发大鼠心肌损伤,其机制可能与抑制心肌组织NF-κB的活性和TLR4的表达有关.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

目的 评价虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响.方法 雄性成年SD大鼠40只,体重190～220 g,随机分为5组(n=8):虫草多糖预先给药组(CP1-3,组)采用灌胃法分别给予虫草多糖1、2、3 g/ks,1次/12 h,连续6次,于最后1次给药后2 h时经股静脉注射内毒素5 mg/kg;急性肺损伤组(ALI组)以生理盐水1ml/100 g代替虫草多糖,其余方法同CP组;对照组(C组)以生理盐水1 ml/100 g代替虫草多糖和内毒素,其余方法同CP组.静脉注射内毒索后6 h时处死大鼠,计算肺湿干重比(W/D)、肺渗透指数(PPI);行支气管肺泡灌洗,对支气管肺泡灌洗液(BALF)行白细胞(WBC)和多形核白细胞(PMN)计数;测定肺组织及BALF肿瘤坏死因子α(TNF-α)水平,并行肺组织病理学评分.结果 与C组比较,ALI组和CP1～3组肺W/D、PPI、WBC和PMN计数、肺组织病理学评分、肺组织和BALF TNNF-α水平均升高(P<0.05或0.01);与ALI组比较,CP1组上述指标差异无统计学意义(P>0.05),CP2,3组上述指标降低(P<0.05);与CP2组比较,CP3组PMN计数、肺组织病理学评分、肺组织和BALFTNF-α水平降低(P<0.05).结论 虫草多糖预先给药可减轻大鼠内毒素性急性肺损伤,且呈剂量依赖性,其机制可能与虫草多糖降低肺组织炎性反应有关.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响

Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.     

罗格列酮预先给药对内毒素诱导大鼠急性肺损伤的保护作用

目的 研究过氧化物酶体增殖物激活受体γ(PPARγ)激动剂-罗格列酮预先给药对内 毒素(LPS)诱导急性肺损伤(ALI)的影响。方法 36只雄性Wistar大鼠,随机分为6组,每组6只:对 照组(DMSO)、ROSI、GW组分别静脉注射10％二甲基亚砜(DMSO)2 ml／kg、罗格列酮0．3 mg／kg或 GW9662 0．3 mg／kg,30 min后静脉注射生理盐水2 ml／kg;LPS、ROSI LPS组分别静脉注射10％DMSO、 罗格列酮0．3 mg／kg,30 min后静脉注射LPS 6 mg／kg;GW ROSI组处理同ROSI LPS组,但在给予罗格 列酮前20min静脉注射GW9662 0．3mg／kg。注射LPS后4 h处死大鼠,光镜下观察肺组织病理学变化, 测定肺组织湿／干重比(W／D)、髓过氧化物酶(MPO)活性、丙二醛(MDA)和一氧化氮(NO)含量,检测肺 组织诱导型NO合酶(iNOS)和硝基酪氨酸(NT)的蛋白表达。结果 与DMSO比较,LPS组肺损伤严 重,W／D、MPO活性、MDA和NO含量升高(P<0．01),肺组织iNOS、NT的蛋白表达增加(P<0．05或 0．01)。与LPS组比较,ROSI LPS组肺损伤明显减轻,W／D、MPO活性、MDA和NO含量降低(P< 0．01),肺组织iNOS和NT的蛋白表达减弱。PPARγ拮抗剂GW9662能逆转罗格列酮的作用。结论 罗格列酮预先给药对内毒素诱导的ALI具有一定的保护作用,其机制与激活PPARγ有关。     

三羟异黄酮预先给药对内毒素诱导大鼠急性肺损伤的保护作用

目的研究三羟异黄酮预先给药对内毒素诱导大鼠急性肺损伤的保护作用及其机制。方法 32只雄性Wistar大鼠,随机分为4组,每组8只:对照组(C组)、三羟异黄酮组(G组)分别腹腔注射生理盐水1 ml／kg、三羟异黄酮50 mg／kg,30 min后,静脉注射生理盐水1 ml／kg;内毒素组(L组)、三羟异黄酮预预先给药组(Gpre组)分别腹腔注射生理盐水1 ml／kg、三羟异黄酮50 mg／kg,30 min后,静脉注射脂多糖6 mg／kg。注射脂多糖后4 h处死动物。测定支气管肺泡灌洗液(BALF)中蛋白浓度、髓过氧化物酶(MPO)活性及中性粒细胞(PMN)数。检测肺组织湿干重比(W／D)、丙二醛(MDA)含量、MPO 活性、肿瘤坏死因子-α(TNF-α)和血红素氧合酶-1(HO-1)mRNA、蛋白表达。观察肺组织病理学的改变。结果与C组比较,L组肺损伤严重、BALF中蛋白、MPO、PMN水平和肺组织W／D、MDA及MPO水平及TNF-α和HO-1 mRNA、蛋白表达水平升高(P<0．05或0．01),G组上述指标差异均无统计学意义(P >0．05);与L组比较,Gpre组除HO-1 mRNA、蛋白表达水平升高(P<0．05)外,其他指标均降低(P< 0．05或0．01)。结论三羟异黄酮预先给药对内毒素诱导大鼠急性肺损伤有一定的保护作用,与抑制PMN在肺组织的聚集、激活,TNF-α表达下调及HO-1表达上调有关。     

姜黄素预先给药对内毒素诱导大鼠急性肺损伤的作用

目的 研究姜黄素预先给药对内毒素诱导大鼠急性肺损伤的作用及其可能机制。方法 48只雄性Wistar大鼠，随机分为4组（n=12），对照组（S组）、姜黄素组（C组）分别静脉注射10％二甲基亚砜（DMSO）2 ml／kg、姜黄素40 mg／kg（溶于DMSO），30 min后静脉注射生理盐水2 ml／kg;内毒素组（L组）、姜黄素预先给药组（C-L组）分别静脉注射10％DMSO 2 ml／kg、姜黄素40 mg／kg，30 min后静脉注射脂多糖（LPS）6 mg／kg。注射LPS后4 h处死动物。每组中6只大鼠在处死前15 min静脉注射伊文思蓝（EB）20mg／kg，用于测定肺组织EB含量，每组的其余大鼠用于测定支气管肺泡灌洗液（BALF）中髓过氧化物酶（MPO）活性和中性粒细胞（PMN）计数及肺组织湿干重比（W／D）、MPO活性、PMN趋化因子-1（CINC-1）mRNA、CINC蛋白表达，并在光镜下观察肺组织病理学改变。结果 与S组比较，L组BALF中MPO、PMN计数和肺组织W／D、EB及MPO、CINC-1 mRNA、CINC蛋白水平升高（P〈0．05或0．01），C组上述指标差异均无统计学意义（P〉0．05）;与L组比较，C-L组上述指标均降低（P〈0．05或0．01）。C-L组肺组织病理学损伤较L组减轻。结论 姜黄素40mg／kg预先给药对内毒素诱导急性肺损伤大鼠肺产生一定的保护作用，与下调肺组织CINC-1的表达进而抑制PMN在肺组织的聚集、激活有关。     

氟比洛芬酯预先给药对大鼠内毒素性急性肺损伤的影响

目的 评价氟比洛芬酯预先给药对大鼠内毒索性急性肺损伤的影响.方法 雄性SD大鼠40只,体重190～220 g,随机分为3组:对照组(C组,n=8)、脂多糖组(LPS组,n=16)和氟比洛芬酯组(FA组,n=16).C组尾静脉注射生理盐水(NS)1 ml;LPS组尾静脉注射LPS 5 mg/kg(1 m1);FA组尾静脉注射氟比洛芬酯6 mg/kg(1 m1)后0.5 h注射LPS 5 mg/kg(1 m1).LPS组和FA组于注射LPS后2、4 h时、C组于注射NS后4 h时,各随机处死8只大鼠,取股动脉血样8 ml,行血气分析;采用放免法测定血清血栓素B2(TXB2)和6-酮-前列腺素F1α(6-keto PGF1α)的浓度;采用ELISA方法测定血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6和IL-10的浓度.取左肺上叶组织,计算肺组织湿/干重比值(W/D)和肺含水量(LC).注射LPS或NS后4 h时取左肺下叶组织,光镜下观察肺组织病理学结果.结果 与C组比较,LPS组PaO2和PaO2/FiO2下降,PaCO2、W/D和LC升高,血清TXB2、6-keto PGF1α浓度和TXB2/6-keto PGF1α比值升高,血清TNF-α、IL-1β和IL-6浓度升高,FA组W/D和LC升高,血清TXB2和6-keto PGF1α浓度升高,血清TNF-α、IL-1β、IL-6和IL-10浓度升高(P<0.05或0.01).与LPS组比较,FA组PaCO2、W/D和LC降低,PaO2和PaO2/FiO2升高,血清TXB2、6-keto PGF1α浓度和TXB2/6-keto PGF1α比值降低,血清IL-1β、IL-6和TNF-α浓度降低,血清IL-10浓度升高(P<0.05).FA组肺组织病理学损伤较LPS组轻.结论 氟比洛芬酯预先给药可减轻内毒素性急性肺损伤,可能与维持血液TXA2和PGI2平衡及抑制炎性反应有关.     

异丙酚对大鼠油酸性急性肺损伤的保护作用

目的观察异丙酚对大鼠油酸性急性肺损伤(ALI)的保护作用及其机制。方法 40只成年雄性 SD 大鼠,体重：250～290g,随机分成5组：正常对照组(Ⅰ组)、ALI 组(Ⅱ组)、4mg·kg~(-1)·h~(-1) 异丙酚治疗组(Ⅲ组)、8mg·kg~(-1)·h~(-1)异丙酚治疗组(Ⅳ组)、16mg·kg~(-1)·h~(-1)异丙酚治疗组(Ⅴ组)；Ⅱ～Ⅴ组静脉注射油酸250μl·kg~(-1)制备大鼠 ALI 模型,然后Ⅲ～Ⅴ组持续静脉输注异丙酚4h 时处死大鼠。测定肺组织 MDA 含量及 MPO、SOD 活性；透射电镜观察肺组织超微结构变化；免疫组化及流式细胞术测定肺组织白介素(IL)-18、IL-10水平；电泳迁移率变动分析技术测定肺组织 NF-(?)B 的表达。结果电镜显示Ⅱ组肺泡Ⅱ型上皮细胞线粒体、粗面内质网及嗜锇性板层小体损伤；Ⅲ～Ⅴ组肺泡Ⅱ型上皮细胞细胞器损伤均有不同程度改善,尤以Ⅳ组效果明显。与Ⅰ组比较,Ⅱ组肺组织 MDA、IL-10、 IL-18、NF-(?)B 水平升高,MPO、SOD 活性降低(P＜0.05)；静脉输注异丙酚使 ALI 大鼠肺组织 MPO、SOD 活性升高,MDA、IL-18、NF-(?)B 水平降低,IL-10水平升高(P＜0.05)。结论 4～16mg·kg~(-1)·h~(-1)异丙酚均可抑制油酸性 ALI 时的氧化应激反应,阻断 NF-(?)B 的活化,减轻肺部炎性反应,对油酸性 ALI 起到一定的保护作用,8mg·kg~(-1)·h~(-1)剂量效果较好。     

利多卡因预先给药对内毒素性急性肺损伤大鼠肺组织层粘连蛋白表达的影响

目的探讨利多卡因预先给药对内毒素性急性肺损伤大鼠肺组织层粘连蛋白（LN）表达的影响。方法健康雄性Wistar大鼠32只,体重220～250g,随机分为4组（n=8）：对照组（C组）、脂多糖组（LPS组）、利多卡因2mg/kg组（L1组）和利多卡因4mg/kg组（L2组）。腹腔注射3%戊巴比妥钠20mg/kg麻醉后,建立尾静脉通路。C组尾静脉输注生理盐水3ml;LPS组依次尾静脉输注2ml生理盐水及1ml LPS（LPS 2mg/kg溶于1ml生理盐水）;利多卡因2、4mg/kg各溶于2ml生理盐水中,L_1组、L_2组分别先输注利多卡因再输注LPS,各组输注速率均为0.05ml/min。输注完毕后5h处死大鼠,迅速取右肺下叶肺组织,测定LN表达;取左肺上叶肺组织,透射电镜观察超微结构。结果与C组比较,LPS组肺组织LN表达上调（P〈0.05）;与LPS组比较,L1组和L2组肺组织LN表达下调（P〈0.05）;与L1组比较,L2组肺组织LN表达下调（P〈0.05）。L1组和L2组肺组织病理损伤较LPS组轻。结论利多卡因预先给药可通过抑制内毒素性急性肺损伤大鼠肺组织LN表达上调,减轻肺部炎性反应及肺损伤,且呈剂量依赖性。     

依达拉奉对大鼠急性肺损伤的干预作用

目的 探讨依达拉奉(EDA)和地塞米松(DXM)对脓毒症大鼠肺损伤的干预作用.方法 50只SD大鼠随机分为假手术组(sham组)、手术对照组(CLP组)、依达拉奉治疗组(EDA组)、地塞米松治疗组(DXM组)和依达拉奉联合地塞米松治疗组(E+D组).采用盲肠结扎穿刺法造脓毒血症大鼠肺损伤模型,药物治疗组造模后立即经阴茎背静脉注射EDA(5mg/kg),DXM(5mg/kg)或EDA(5mg/kg)+DXM(5mg/kg);另两组均以等量生理盐水代替.结果 与 sham组相比,CLP组大鼠病理评分,肺干湿重比(W/D),肺泡通透指数(LPI),肺组织中MPO,MDA,IL-6,TNF-α,肺组织中凋亡细胞数量明显增加,而肺组织中T-AOC活性明显减轻.应用地塞米松,依达拉奉药物干预后,上述指标均有不同程度的改善.两种药物联合应用与单个药物应用相比,这些指标有更明显的改善.结论 依达拉奉预处理对脓毒症诱导的肺损伤有明显的防治效果,减轻急性肺损伤的机制可能是通过减少氧自由基产物,减少炎性细胞因子浓度.依达拉奉和地塞米松联合应用对肺损伤有更好的治疗效果.     

微量肺源性内毒素对大鼠呼吸机相关性肺损伤的影响

目的 评价微量肺源性内毒素对大鼠呼吸机相关性肺损伤的影响.方法 成年雄性SD大鼠32只,体重370～390 g,随机分为4组(n=8):自主呼吸组(C组)、内毒素+自主呼吸组(LC组)、机械通气组(M组)和内毒素+机械通气组(LM组).LC组和LM组气管内滴入内毒素100 μg/kg;M组和LM组行机械通气,潮气量20 ml/kg,呼气末正压0,1:E 1:1,维持P_(ET)CO_235～45 mm Hg;C组和LC组保持自主呼吸.于机械通气前、机械通气1、2和3 h时行血气分析,并记录血液动力学指标.机械通气3 h时放血处死大鼠,测定肺组织病理学损伤评分、湿/干重比(W/D比)、支气管肺泡灌洗液(BALF)中白细胞计数和肺蛋白透性系数,采用ELISA法检测血浆TNF-α和巨噬细胞炎性蛋白-2(MIP-2)的浓度.C组和M组采用RT-PCR法测定肺组织CD14 mRNA的表达水平,免疫组化法测定BALF中CD14的表达水平.结果 C组和M组血浆中未检测到TNF-α;与C组比较,LC组肺组织病理学损伤评分、W/D比、BALF中自细胞计数、肺蛋白透性系数和血浆MIP-2浓度差异无统计学意义(P>0.05),血浆TNF-α浓度升高,M组肺组织病理学损伤评分、BALF中白细胞计数和血浆MIP-2浓度升高,LM组肺组织病理学损伤评分、W/D比和BALF中白细胞计数、肺蛋白透性系数、血浆MIP-2和TNF-α的浓度升高(P<0.05或0.01);与M组比较,LM组肺组织病理学损伤评分、W/D比、BALF中自细胞计数、肺蛋白透性系数、血浆MIP-2和TNF-α的浓度升高(P<0.05或0.01).与C组比较,M组BALF中CD14表达和肺组织CD14 mRNA表达上调(P相似文献