Different effects on bone strength and cell differentiation in pre pubertal caloric restriction versus hypothalamic suppression

Hypothalamic amenorrhea and energy restriction during puberty affect peak bone mass accrual. One hypothesis suggests energy restriction alters hypothalamic function resulting in suppressed estradiol levels leading to bone loss. However, both positive and negative results have been reported regarding energy restriction and bone strength. Therefore, the purpose of this study was to investigate energy restriction and hypothalamic suppression during pubertal onset on bone mechanical strength and the osteogenic capacity of bone marrow-derived cells in two models: female rats treated with gonadotropin releasing hormone antagonists (GnRH-a) or 30% energy restriction. At 23 days of age, female Sprague Dawley rats were assigned to three groups: control group (C, n = 10), GnRH-a group (n = 10), and Energy Restriction (ER, n = 12) group. GnRH-a animals received daily injections for 27 days. The animals in the ER group received 70% of the control animals' intake. After sacrifice (50 days of age), body weight, uterine and muscle weights were measured. Bone marrow-derived stromal cells were cultured and assayed for proliferation and differentiation into osteoblasts. Outcome measures included bone strength, bone histomorphometry and architecture, serum IGF-1 and osteocalcin. GnRH-a suppressed uterine weight, decreased osteoblast proliferation, bone strength, trabecular bone volume and architecture compared to control. Elevated serum IGF-1 and osteocalcin levels and body weight were found. The ER model had an increase in osteoblast proliferation compared to the GnRH-a group, similar bone strength relative to body weight and increased trabecular bone volume in the lumbar spine compared to control. The ER animals were smaller but had developed bone strength sufficient for their size. In contrast, suppressed estradiol via hypothalamic suppression resulted in bone strength deficits and trabecular bone volume loss. In summary, our results support the hypothesis that during periods of nutritional stress the increased vertebral bone volume may be an adaptive mechanism to store mineral which differs from suppressed estradiol resulting from hypothalamic suppression.     

地塞米松对围术期肾功能损害的保护作用

目的探讨地塞米松对围术期肾功能损害的保护作用。方法择期全麻下行胃肠道手术患者40例,随机均分为两组。地塞米松组(Ⅰ组)术前30min静注地塞米松10mg,对照组(Ⅱ组),术前未给地塞米松。分别于麻醉诱导前(T0)、手术结束时(T1)、术后第1天(T2)、第3天(T3)和第5天(T4)留取尿标本,测定α1-微球蛋白(α1-MG)、微量白蛋白(Alb)及β-N-乙酰(基)-D-氨基葡萄糖苷酶(NAG)含量,在同一时点抽静脉血测定血肌酐(Cr)、尿素氮(BUN)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和肿瘤坏死因子可溶性受体Ⅰ(sTNF-R-I)浓度。结果两组T1时TNF-α浓度均明显高于T0时(P<0.05)。T1~T4时Ⅱ组IL-6浓度均较Ⅰ组高(P<0.05)。T2、T3时Ⅱ组sTNF-R-I浓度均较Ⅰ组低(P<0.05)。两组血浆Cr和BUN浓度变化不明显,两组间比较差异无统计学意义。T1~T3时两组α1-MG/Cr与NAG/Cr均高于T0时(P<0.01),但Ⅱ组T4时α1-MG/Cr、NAG/Cr仍然高于T0时(P<0.01)。T1~T4时Ⅱ组NAG/Cr明显高于Ⅰ组(P<0.05),两组间α1-MG/Cr比较差异无统计学意义;T1、T2时两组尿Alb/Cr均较T0时明显增高(P<0.01),Ⅱ组于T1时高于Ⅰ组(P<0.05)。结论围术期存在IL-6和sTNF-R-I失衡,出现围术期亚临床状态的肾功能受损。地塞米松在一定程度上减少血中IL-6的释放,促进sTNF-R-I的生成,可能有利于减轻肾功能的损害。     

前列腺素E1对大鼠肝移植肾功能的保护作用

目的探讨术中应用前列腺素E1（prostaglandin E1，PGE1）对大鼠肝移植肾功能的保护作用。方法大鼠原位肝移植术中经颈内静脉灌注PGE1为治疗组，生理盐水和空白为对照组，观察术后1周存活率、1h的尿量，测定血浆肌酐、尿素氮和肾组织中丙二醛（malondjaldehyde，MDA）、谷胱甘肽（glutathione，GSH）含量，肾组织病理检查。结果PGE1治疗组术后1h尿量较对照组明显增加，肌酐和尿素氮水平均较对照组降低，PGE1治疗组肾组织中GSH含量显著高于两对照组，MDA含量低于两对照组。病理检查PGE1治疗组肾脏组织形态学损伤明显减轻。结论术中应用PGE1能显著改善大鼠肝移植后的肾功能，其机制可能与对抗氧自由基损伤作用有关。     

Impact of pneumoperitoneum on renal perfusion and excretory function: beneficial effects of nitroglycerine

Background  Increased intra-abdominal pressure (IAP) (pneumoperitoneum) during laparoscopic surgery may result in adverse effects on kidney function. The mechanisms underlying this phenomenon have not been fully determined. Objective  The present study was designed to: (1) investigate the effects of incremental increases in IAP on renal function in normal rats and (2) evaluate whether the nitric oxide (NO) system is involved in renal dysfunction characterizing pneumoperitoneum. Methods  Male rats were organized into two groups. The first group was subjected to IAP of 0 (baseline), 7 or 14 mmHg, over 1 h for each pressure, followed by a deflation period of 60 min (recovery). Two additional groups were pretreated with: (1) non-depressor dose of nitroglycerine (NTG) and (2) nitro-L-arginine-methylester (L-NAME), an NO synthase inhibitor, before applying 14 mmHg for 1 h. Urine flow rate (V), Na+ excretion (U_NaV), glomerular filtration rate (GFR), renal plasma flow (RPF), and blood pressure were determined throughout the experiments. Results  There were no significant changes in V, U_NaV, GFR, and RPF during 7 mmHg insufflation. However, significant reductions in these parameters were observed during 14 mmHg: V from 8.49 ± 0.92 to 6.12 ± 0.54 μl/min, U_NaV from 1.29 ± 0.28 to 0.39 ± 0.09 μEq/min, and FE_Na from 0.37 ± 0.11 to 0.27 ± 0.04%. These alterations in excretory functions were associated with a considerable decline in GFR from 1.85 ± 0.09 to 0.88 ± 0.09 ml/min, p < 0.05, (−46.3 ± 5.2% from baseline) and RPF from 8.66 ± 0.62 to 4.33 ± 0.49 ml/min, p < 0.05, (−51.93 ± 5.24% from baseline), without a significant change in mean arterial blood pressure (MAP). When the animals were pretreated with NTG, the adverse effects of pneumoperitoneum on V, U_NaV, GFR, and RPF were substantially improved, suggesting that NO system plays a beneficial counter-regulatory role during laparoscopy. In line with this notion, pretreatment with L-NAME remarkably aggravated pneumoperitoneum-induced renal hypoperfusion and dysfunction. Conclusion  Decreased renal perfusion and function are induced by IAP pressure of 14 mmHg. These adverse effects are probably related to interference with the NO system, and could be partially ameliorated by pretreatment with NTG. Bishara Bishara and Tony Karram contributed equally.     

Anti-nociceptive effects of caloric restriction on neuropathic pain in rats involves silent information regulator 1

Background

Caloric restriction (CR) increases both average and maximum lifespan, retards physiological signs of ageing, and delays the onset of several diseases and may mediate neuropathic pain. Neuropathic pain seriously affects the quality of life of patients. In this study, we investigated whether CR exerts anti-nociceptive effects on neuropathic pain, and probed its potential mechanisms.

Influence of renal nerves on renal function during development

The present review summarizes recent studies describing the role of renal sympathetic innervation in the regulation of renal function during development. The afferent renal innervation appears early during fetal life and probably precedes the development of efferent renal nerves. There is suggestive evidence that renal nerves are required for the proper development of the kidney and that neurotrophic growth factors play an important role in renal embryogenesis and in renal tubular differentiation. Renal sympathetic innervation modulates renal hemodynamics early during development. Renal nerve stimulation during -adrenoceptor blockade produces renal vasodilation in fetal and newborn animals but not in adults. Unlike the effect of renal nerves on fetal renal hemodynamics which is observed in the young fetus, the role of renal sympathetic nerves in modulating fluid and electrolyte homeostasis seems to develop during late gestation. Recent studies have also shown that renal nerves play an important role in regulating renin secretion during the transition from fetal to newborn life. For example, renal denervation during fetal life suppressed the physiological rise in plasma renin activity associated with delivery and decreased renal renin mRNA levels after birth. Taken together, these studies suggest that renal nerves influence fetal renal development and that the influence of renal sympathetic innervation on renal hemodynamics and function changes with maturation.     

The effect of bariatric surgery on renal function

BackgroundObesity is associated with albuminuria and impaired renal function. We previously reported on 38 nondiabetics with improved albuminuria after Roux-en-Y gastric bypass (RYGB).MethodsOur objectives were to evaluate changes in renal function, urinary albumin-to-creatinine ratio (UACR), and glomerular filtration rate (GFR) in a larger cohort of patients with normal or mildly impaired renal function, undergoing RYGB or sleeve gastrectomy at 1 year postop. This was a retrospective study. Inclusions: patients with preoperative and 1 year postoperative serum and urine albumin and creatinine and weight (kg). Exclusions: preop chronic kidney disease (CKD)≥Stage 3 or macroalbuminuria (UACR≥300 mg/g). Primary outcomes: changes in UACR and estimated GFR (eGFR) at 1 year. The setting was in a public hospital in New York City, 2004–2011.Results158 patients met inclusion criteria; 91.8% female; mean age 40.8 years; 84.2% white Hispanic, 14.6 % black. Hypertension was present in 43.0%, diabetes mellitus in 28.5%. UACR was 21.5±3.2 mg/g, decreasing to 10.2±1.2 mg/g at 1 year (P<.0001). Microalbuminuria was present in 22/158 patients (14%) preop, resolving in 82% at 1 year; pre- versus 1 year postop eGFR, 97.5±2.2 versus 87.1±2.0 mL/min (P<.0001). Hyperfiltration was present in 8.2% preop, decreasing to 4.4% 1 year postop.ConclusionIn this mainly female minority population, UACR decreased within the normal range, while eGFR decreased from normal to the range for Stage 2 CKD at 1 year postop. Microalbuminuria resolved in most affected and hyperfiltration resolved in nearly half of those affected. This study is limited by its retrospective nature. Prospective studies should be performed.     

乌司他丁对腔静脉成形肝移植术肾功能的保护作用

目的观察腔静脉成形肝移植术患者肾功能的变化与乌司他丁对肾功能的保护作用。方法71例腔静脉成形肝移植术患者,随机给予乌司他丁30万IU或等量生理盐水,持续静脉输注1h,新肝期再重复一次。根据腔静脉平均阻断时间分成≤62min组,其中用乌司他丁23例(U1组)、生理盐水18例(C1组);>62min组,其中用乌司他丁13例(U2组)、生理盐水17例(C2组)。监测血流动力学、血气、生化指标、肾功能指标、凝血功能、体温、尿量及出血量等。结果下腔静脉阻断(IVC)时间为(62.3±11.2)min。与术前相比,所有患者术后24h血尿素氮(BUN)明显升高(P<0.01),血肌酐(Cr)在新肝期1h、术毕、术后24h均明显升高(P<0.01)。术后24hBUN、CrU1组明显低于C1组(P<0.01),U2组亦明显低于C2组(P<0.01)。而U1与U2组、C1与C2组BUN、Cr差异无统计学意义。结论腔静脉成形术肝移植术对肾功能有明显损伤,术中使用乌司他丁对肾脏有一定程度的保护作用。     

Management of atherosclerotic renovascular disease: the effect of renal artery stenting on renal function and blood pressure

In the last few years, renal artery stenting has gradually evolved into one of the most important therapeutic modalities in the management of atherosclerotic renovascular disease. Stenting is nowadays preferred by a steadily increasing number of physicians, not only because of its significant contribution to blood pressure control, but also because of its documented ability to maintain, and even improve, renal function. At the same time, procedure-related morbidity and mortality rates are extremely low, while recurrent stenosis rates have been repeatedly reported to be␣minimal. Percutaneous transluminal renal angioplasty (PTRA) and stenting are nowadays considered by many physicians to be the treatment of choice for atherosclerotic renovascular disease.     

Effects of pre- and postnatal cysteamine exposure on renal function in the rat

The safety of cysteamine after renal transplantation and during pregnancy is an important issue, since girls with cystinosis are in better health on cysteamine therapy and thus more likely to become pregnant. In the first study, cysteamine was given to pregnant rats on days 6.5–18.5 post conception in oral doses of 0, 37.5, 75, 100, and 150 mg/kg per day. The dams were sacrificed on day 20.5, the fetal kidneys removed and prepared for histological examination. In the second study, cysteamine was given to dams on days 6.5–19.5 post conception in oral doses of 0, 37.5, 50, and 75 mg/kg per day. Dams were allowed to give birth naturally and pups were given cysteamine on days 4–21 to yield the same oral doses of cysteamine given to the dam. Renal function was evaluated on day 35. Histological examination of fetal kidneys revealed no changes even in kidneys from fetuses with growth retardation and malformations. Furthermore, there were no alterations in renal function in offspring on day 35. These findings demonstrate that cysteamine therapy does not affect renal development in the rat. Further investigations will be required to prove whether cysteamine therapy has the potential to affect renal development in the human. Received: 25 September 1998 / Revised: 17 November 1998 / Accepted: 13 December 1998     

Pathophysiology of renal function and its effect on isotope studies in the workup of hydronephrosis

Understanding renal maturation and growth is essential before adequate interpretation of radionuclide renal studies can be done. The expectation that an immature kidney draining into a dilated renal pelvis will show good washout on a diuretic renogram within 20 or 40 min is unreasonable. A physiological explanation for this is given.     

早期肠道喂养对烧伤后肾功能的保护作用及其可能机制

目的　研究早期肠道喂养 (EEF)对严重烧伤大鼠肾脏功能的保护作用及其可能机制。　方法　采用Wistar大鼠 30 %TBSA烫伤模型 ,动态观察了EEF对伤后胃黏膜内pH(pHi)、门静脉内毒素水平、肾组织含水量、血浆肿瘤坏死因子 (TNFα)、尿素氮 (BUN)、肌酐 (BCr)含量及内生肌酐清除率 (CCr)变化的影响。　结果　与烧伤对照组相比 ,EEF可明显遏制pHi降低、并减少门静脉内毒素、血浆TNFα及肾组织含水量 ;EEF不降低伤后BUN、BCr的浓度 ,但可使CCr增加 4.6 7倍 [( 16 .43± 2 .90 )vs ( 3.5 2± 0 .79) ,P <0 .0 1]。　结论　EEF对严重烧伤大鼠肾功能损害具有明显的改善作用 ,其机制可能与减少肠源性内毒素移位及炎性介质的释放有关     

The suppressive effects of dietary protein restriction on the progression of renal impairment in OLETF rats

Background The suppressive effect of dietary protein restriction on the progression of diabetic nephropathy remains controversial. We investigated the effects of protein and energy restrictions on both albuminuria and morphology using diabetic-prone Otsuka Long-Evans Tokushima fatty (OLETF) rats. Methods In this study, male OLETF rats were divided into two groups according to their energy intake. They were then further divided into three subgroups based on their amount of dietary protein, which ranged between 10% and 30% of their total intake. Urinary albumin excretion (UAE) was used as a marker of renal impairment, and body weight fasting (F) and postchallenge (P), blood glucose (BG) levels, and systolic blood pressure (SBP) were all measured during various experimental periods up to 28 weeks of age. Results The OLETF rats fed with the high-calorie diet started to gain weight at 12 weeks, and their FBG and PBG were elevated at 22 weeks, while SBP did not differ between the two groups. In addition, UAE increased significantly in the rats fed with the high-calorie diet. However, the increasing rates of UAE with age were higher in the rats with a higher protein diet within the same energy groups. UAE correlated well with the amounts of dietary energy and protein at 16 and 28 weeks of age, while it correlated with both the FBG and PBG at only 28 weeks of age. A linear regression analysis, using the data obtained at 28 weeks, showed that the amount of protein intake and FBG explained 63.4% and 23.9% of the variation in UAE, respectively. Histological studies revealed that protein and energy restriction markedly reduced the sclerotic changes of the glomeruli. Conclusion Dietary protein restriction starting very early in the life of OLETF rats, in combination with energy restriction, clearly suppressed UAE and the typical morphological changes that otherwise occurred at around 16 weeks of age. This method also seemed to be more effective than energy restriction alone in slowing down any increase in UAE. The influence of BG levels on UAE was lower at an early age, while it became an increasingly important factor at later ages in the experimental rat model.     

经皮肾镜对复杂性肾结石患者术后早期肾功能的影响

目的探讨经皮肾镜碎石取石术(PCNL)对复杂性肾结石患者术后早期肾功能的影响,并评估患者术后肾功能恶化的危险因素。方法将77例自2017年1月至2018年8月在我院行PCNL的复杂性肾结石患者,根据术前基线肾功能分为肾功能正常(血肌酐<115μmol·L^-1)的A组和肾功能异常(血肌酐≥115μmol·L^-1)的B组,每组又根据手术通道数目,分为单通道组(通道数目=1)和多通道组(通道数目≥2),记录患者术前及术后24 h内的血肌酐及其他评价肾功能的指标,以此对患者术后肾功能进行评估。同时记录并评估可能对肾功能改变产生影响的相关因素。结果 A组中,仅在多通道患者中术后胱抑素C水平较术前升高,且差异有统计学意义(P<0.05)。其他指标几乎保持稳定状态(P>0.05)。B组中,单通道患者与A组相似,各指标基本保持稳定(P>0.05)。多通道患者术后血肌酐及胱抑素C水平显著上升,估算肾小球滤过率显著下降,差异有统计学意义(P<0.05)。导致肾功能恶化的独立危险因素包括术前高浓度血肌酐、多通道、糖尿病和高血压。结论多通道PCNL对肾功能不全患者的肾功能早期影响较大,多通道、术前肾损伤、糖尿病及高血压是肾功能减退的潜在危险因素。     

运动训练联合基因治疗对肾性高血压大鼠肾功能的影响

目的观察运动训练联合β1肾上腺素能受体基因治疗对肾性高血压大鼠血压、肾功能、肾脏前肾素原mRNA、肾脏β1受体mRNA和蛋白的影响，探讨其改善肾功能的机制。方法两肾一夹法制作肾性高血压模型，基因治疗采用经鼠尾静脉注射阳离子脂质体与β1反义寡核苷酸方法。检测大鼠血压、肾功能变化。半定量RT—PCR测定肾脏β1受体mRNA、前肾素原mRNA水平。Western印迹法测检肾脏β1受体的蛋白水平。结果与模型组比较，运动联合基因治疗可使血压下降并维持4周，血压下降最高达41mmHg；尿蛋白量[（45．82±6．56）比（29．12±5．22）mg／L，P〈0．01】、BUN[（13．10±2．62）比（9．05±1．84）mmol／L，P〈0．05]显著降低（P〈0．01，P〈0．05）；内生肌酐清除率显著升高（P〈0．01）；前肾素原mRNA、β1受体mRNA、蛋白表达水平显著降低（P〈0．05）。结论运动训练联合β1受体反义基因治疗可以明显地降低血压，改善肾功能；且运动训练可以增强基因治疗对β1受体mRNA和蛋白的抑制作用，在转录和翻译水平抑制过度激活的β1受体的表达。     

Development of injury in a rat model of chronic renal allograft rejection: effect of dietary protein restriction

Non-allogeneic factors such as increased nephron “workload” may contribute to chronic renal allograft rejection. Reducing dietary protein from 20 % to 8 % was tested in a model of chronic rejection: Dark Agouti kidney to Albino Surgery recipient, “tolerised” by previous donor blood transfusions. Survival, weight gain, serum creatinine concentration and creatinine clearance were similar for both groups at all times. Urinary protein was significantly (P < 0.05) lower in the low-protein (LP) group 1 month after transplantation. After 3 and 6 months, both groups demonstrated mild chronic rejection. After 6 months, tubular atrophy was significantly (P < 0.05) less in the LP group and interstitial fibrosis was marginally reduced. Glomerular hypertrophy, glomerular sclerosis, tubular dilatation, leucocyte infiltration, adhesion molecule expression and TGF-β ₁ mRNA expression were similarly increased in both groups. Thus, reducing dietary protein to 8 % lowered urinary protein, but did not significantly affect the development of chronic rejection in renal allografts beyond affording a degree of protection from tubulointerstitial damage. Received: 17 June 1998 Accepted: 23 September 1998     

药典法定剂量关木通对大鼠肾功能及间质结构影响的研究

目的观察药典法定剂量关木通水煎剂对大鼠肾功能及肾间质结构的影响.方法用药典规定剂量的关木通水煎剂给大鼠灌胃(1g/kg,约相当于人类剂量0.1g/kg),每日1次,共两个月.期间观察各项肾功能指标、肾脏组织形态学改变及细胞外基质(纤连蛋白)分布情况.结果关木通给药组的血肌酐水平、尿酶(NAG和γ-GT)活性、尿蛋白、尿糖与正常组大鼠相比无统计学差异.关木通给药组大鼠的肾组织形态结构在光镜下未见明显异常改变,其纤连蛋白的分布与正常组完全相同.结论药典法定剂量的关木通水煎剂对大鼠肾功能及肾间质结构无明显的不利影响.     

Early postoperative renal function following renal transplantation surgery: effect of anesthetic technique

Purpose. The use of continuous epidural anesthesia in patients with chronic renal failure is rare and controversial. In this study, we compared the effects of epidural versus general anesthesia on early postoperative renal function in patients who underwent renal transplantation surgery. Methods. Sixty-eight adult patients were prospectively randomized to two groups. Group 1 (n-37) received epidural anesthesia with bupivacaine and fentanyl, and group 2 (n-31) received general anesthesia with nitrous oxide and isoflurane. The patients' renal function was compared both with qualitative scintigraphic analysis (kidney perfusion, concentration, and excretion capabilities) and biochemically [serum sodium, potassium, creatinine, and blood urea nitrogen) (BUN)] within the first postoperative week. Results. Patient demographics were similar in the two groups, and the scintigraphic and biochemical evaluations were also comparable. The time of the first appearance of Tc-99m diethylene triamine pentaacetic acid (DTPA) was within normal limits in 75.7% of patients in group 1 and 61.3% of those in group 2. The number of patients with normal peak/background activity and 20 min/peak activity were 15 (40.5%) and 19 (51.4%), respectively, in group 1, and 12 (38.7%) and 15 (48.4%) in group 2 (P > 0.05 for both). The levels of serum creatinine and urea in both groups decreased within days postsurgery compared with preoperative levels (P < 0.05), but the changes were similar in the two groups (P > 0.05). A similar number of patients in both groups were treated for acute rejection (P > 0.05). Conclusion. Our results demonstrate the safe use of both anesthetic techniques in renal transplantation surgery. Received: December 25, 2000 / Accepted: November 5, 2001     

大鼠肾移植模型手术改良技巧探讨

目的  探讨大鼠肾移植模型手术的改良方法。方法  供体Sprague-Dawley(SD)大鼠21只, 受体Wistar大鼠42只。采用双侧供肾。受体左肾切除后借助自制导管, 行受体肾动脉与供体肾动脉、受体肾静脉与供体下腔静脉端端吻合, 供体输尿管带膀胱瓣与受体膀胱吻合, 最后切除右肾, 腹腔内注入头孢米诺10 mg, 关腹。记录手术时间, 动、静脉吻合时间, 冷、热缺血时间等手术数据; 术后大鼠存活3 d认为模型建立成功, 计算建模成功率, 分析死亡原因。结果  供体手术时间为(32.7±5.6)min, 供肾修整时间为(4.2±1.1)min。受体手术时间为(42.3±4.9)min, 其中动脉吻合时间为(10.1±3.2)min, 静脉吻合时间为(13.9±2.5)min, 尿路重建时间为(6.3±1.4)min。热缺血时间为(5.4±1.8)s, 冷缺血时间为(56.2±7.3)min。42只受体大鼠中, 建模成功40只, 成功率为95%。另2只受体大鼠死亡, 其中1只死于血管吻合口出血, 1只死于尿瘘引致的腹膜炎。结论  采用改良的血管端端吻合法建立大鼠肾移植模型具有操作简单、手术时间短、成功率高的特点。     

输尿管膀胱再植术对患侧肾功能的影响

目的：探讨输尿管膀胱再植术对患侧肾功能的影响。方法：回顾性筛选分析手术前后分别行ECT肾小球滤过率（GFR）测定，对侧肾输尿管正常，膀胱或肾无其他病变的输尿管膀胱再植术患者临床资料。结果：在33例符合筛选条件患者中，Cohen-Ahmed术式26例，Politano-Ledbetter术式5例，Lich-Gregoir术式2例，21例（63．6％）GFR术后近期较术前降低（平均5．3％），结论：大部分输尿管膀胱再植术病例，术后近期，患肾功能轻度受损。