西宁地区恶性肿瘤患者的红细胞免疫功能研究

目的：研究西宁地区（海拔2260m）恶性肿瘤的红细胞免疫功能的相关内容。方法：按文献^[2]介绍采用郭峰建立的最常用的三种检测指标和方法。结果：肿瘤病人红细胞C3b受体花环R-C3bR、红细胞免疫粘附肿瘤细胞花环R-TRR低下，与健康人之间有显著性差别，红细胞免疫复合物IC花环R-ICR无统计学差别，肿瘤患者的R-TRR男女之间有显著性差别。各种肿瘤之间三种花环率无统计学差别。化疗前后三种花环率无统计学差别。肿瘤无转移与有转移三种花环率无统计学差别。手术前后三种花环率无统计学差别。结论：西宁地区恶性肿瘤病人的红细胞免疫功能R-C3bR、R-TRR低下，分别为13．4762±3．597，12．0543±5．6095，R-ICR为4.6131±3．4010，与健康人无统计学差别。     

高原肺水肿患者红细胞免疫粘附功能变化及其意义

本文观察了29例肺水肿患者的红细胞免疫粘附功能及调节因子水平，与34例初入高原健康人及25例急性高原反应患者进行了比较，他们分别于治疗前、恢复期、痊愈期抽血通过红细胞C3b受体花环率试验（RCR）、红细胞免疫复合物花环率试验（RICR）、红细胞C3b受体花环促进率试验（RFER）、红细胞C3b受体花环抑制率试验（RFIR）和PEG沉淀法检测，发现高原肺水肿患者RCR、RICR及RFER均降低、RFIR升高，血清中CIC则升高，急性高原反应患者RCR和血清中CIC接近对照组.RICR明显升高，RFER和RFIR均与正常对照组接近，表明高原肺水…     

高原红细胞增多症病人红细胞滤过指数和红细胞免疫粘附功能的研究

作者检测了３３００ｍ处高原红细胞增多症和健康人的红细胞滤过指数、红细胞Ｃ３ｂ受体花环率和红细胞免疫复合物花环率,并按４０岁为界限分组。结果显示：高原红细胞增多症患者红细胞滤过指数明显高于健康组;红细胞Ｃ３ｂ受体花环率明显低于健康组,而红细胞免疫复合物花环率显著高于健康组;相关分析表明：高原红细胞增多症患者红细胞滤过指数与红细胞Ｃ３ｂ受体花环率呈负相关（ｒ＝－０９０９７）,而与红细胞免疫复合物花环率呈正相关（ｒ＝０７４４７）。研究结果表明：高原低氧环境下,红细胞变形能力降低是导致高原红细胞增多…     

高原地区进展期胃癌患者红细胞免疫功能和T淋巴细胞亚群的研究

目的：研究高原地区进展期胃癌（AGC）患者的红细胞免疫功能和淋巴细胞免疫功能;方法：对我科住院手术治疗的32例进展期胃癌患者作了红细胞C3b受体花环（RBC－C3bRR)、红细胞免疫复合物花环（RBC－ICR）及淋巴细胞亚群CD3、CD4、CD8、CD4／CD8的检测,并与20例正常人结果进行比较;结果：高原地区进展期胃癌患者RBC－C3bRR,CD3,CD4,CD8、CD4／CD8比值均低于正常人（P＜0．01）,RBCICR高于正常人（P＜0．01）;结论：高原地区进展期胃癌患者红细胞免疫系统与淋巴细胞免疫系统均处于抑制状态,2个免疫系统的改变有着密切的关系,对高原地区进展期癌患者应给予免疫支持。     

高原地区胃癌患者红细胞免疫功能变化的临床研究

目的：探讨高原地区胃癌患者红细胞免疫功能情况;方法：分别测定胃癌病人在手术前后红细胞Ｃ３ｂ花环形成试验受体（Ｃ３ｂ ＲＲＴ）以及红细胞免疫复合物花环试验（ＩＣＲＴ）,其结果与正常人做对比;结果：胃癌病人红细胞Ｃ３ｂ花环促进率术前下降,明显低于正常对照组（Ｐ〈０．０１）,术后回升。红细胞免疫复合物花环率在手术前高于正常对照组,术后明显下降,与术前相比有明显差异（Ｐ〈０．０１）;结论：高原地区居民患胃     

西宁地区正常人的红细胞免疫功能研究

目的:了解掌握西宁地区正常人红细胞免疫功能的正常值。方法:按文献[1]采用郭峰建立的最常用的三种检测指标和方法。结果:西宁地区健康人三种红细胞花环率(%):补体C3b受体花环率(R-C3bR)、红细胞免疫复合物IC花环率(R-ICR)、红细胞免疫粘附肿瘤细胞花环率(R-TRR)分别为13.47±3.05,3.61±1.46,31.32±6.06。结论:西宁地区正常人三种花环率正常值与外省各地区的正常值有显著差别,数值较外地低下。三种花环率与性别之间无显著性差别。     

中度高原地区体育锻炼对老年人红细胞免疫和CIC的影响

观察中度高原（２２６０ｍ）地区，体育锻炼对老年人红细胞克疫粘附（ＲＣＩＡ）功能和循环免疫复合物（ＣＩＣ）水平的影响，方法：ＲＣＩＡ功能，采用红细胞Ｃ２ｂ受体花环率和复合物花环率试验，ＣＩＣ采用ＰＥＧ半定量法。结果：运动组老年人红细胞Ｃ３ｂ受体花环率明显升高（Ｐ＜０．０１），而且ＣＩＣ明显下降（Ｐ＜０．０２）．结论：中度高原地区老年人体育锻炼可增强红细胞免疫粘附功能，使红细胞Ｃ３ｂ全体花环率升高，减少由ＣＩＣ所致的疾病，有利于抗衰老。     

热习服动物红细胞免疫变化的特点

目的 探讨高温环境和热习服对机体红细胞免疫功能的影响程度,以了解热损伤及热适应机理,探索机体对高热防护措施有重要意义。方法 采用郭峰建立的酵母花环试验,对26只日本大耳白兔分组进行红细胞C3b受体花环及红细胞IC花环检测。结果 高温组红细胞C3b受体花环率和红细胞IC花环率与对照组较明显升高(P<0.01),而习服组动物两种花环已基本恢复至正常对照(P>0.05)。结论 高温环境对机体红细胞免疫有一定影响,而热适应性锻炼可提高机体的热耐受能力。     

高原附红细胞体病患者红细胞免疫功能变化观察

附红细胞体病(附红体病,Eperythrozoonsis)是由附红细胞体(附红体,Eperythrozoon)感染机体引起的人畜共患的传染病。本文旨在了解高原环境条件下附红细胞体病患者红细胞免疫功能状况及血清中红细胞免疫功能调节因子水平。作者采用酵母花环法检测了高原19例附红细胞体病患者红细胞C3b受体(RBC-C3b)花环率、红细胞携带免疫复合物(RBC-IC)花环率、血清中红细胞免疫功能促进因子(RFER)和血清中红细胞免疫功能抑制因子(RFIR),并对其进行了动态观察,与同海拔地区40例健康人进行了比较。结果:RBC-C3b花环率、RBC-IC花环率和RFER低于…     

食管癌患者手术前后红细胞免疫功能研究

本文报道20例食管癌患者手术前后红细胞免疫功能的检测结果。发现食管癌组红细胞C_3b受体花环率(RBC C_3b RR)明显低于对照组(P<0.01),食管癌组红细胞免疫复合物花环率(RBC-ICR)低于对照组(P<0.05)。本文讨论了食管癌患者红细胞免疫功能动态观察情况。指出食管癌可抑制红细胞免疫功能,食管癌切除后红细胞免疫功能可逐渐恢复,提示红细胞免疫功能可能成为食管癌手术后病情变化的指标之一。     

114例消化系统恶性肿瘤患者红细胞免疫功能测定的探讨

本文对114例各类消化系统恶性肿瘤及48例健康成人红细胞免疫功能调节因子进行了观察比较,经统计学处理与正常人相比,消化系统各肿瘤红细胞C_(3b)受体花环促进率(RFER)降低,而红细胞C_(3b)受体花环抑制率(RFIR)明显升高(P<0.01)。这不仅与肿瘤细胞产生的某些抑制因子有关,而且与机体红细胞免疫功能调节紊乱和红细胞粘附IC增多及SOD活性下降有关。     

高压氧对急性颅脑损伤患者红细胞免疫功能的影响

目的探讨高压氧（ｈｙｐｅｒｂａｒｉｃｏｘｙｇｅｎ,ＨＢＯ）治疗对急性颅脑损伤患者红细胞免疫功能的影响。方法对３４例急性颅脑损伤患者行ＨＢＯ辅助治疗（０．２ＭＰａ下吸纯氧）,观察其治疗前后红细胞Ｃ３ｂ受体花环率（ｒｅｄｂｌｏｏｄｃｅｌＣ３ｂｒｅｃｅｐｔｏｒｒｏｓｅｔｅｒａｔｅ,ＲＢＣ－Ｃ３ｂＲＲＲ）和红细胞免疫复合物花环率（ｒｅｄｂｌｏｏｄｃｅｌｉｍｍｕｎｅｃｏｍｐｌｅｘｒｏｓｅｔｅｒａｔｅ,ＲＢＣ－ＩＣＲＲ）,以及肿瘤细胞－红细胞花环率（ｔｕｍｏｒｃｅｌ－ｅｒｙ－ｔｈｒｏｃｙｔｅｒｏｓｅｔｔｅｒａｔｅ,ＴＣＥＲＲ）的变化,并与４５名健康者作对照。结果ＨＢＯ治疗后患者ＲＢＣ－Ｃ３ｂＲＲＲ和ＴＣＥＲＲ明显高于治疗前,而ＲＢＣ－ＩＣＲＲ则明显低于治疗前（Ｐ＜０．０１）;与正常对照组相比,ＨＢＯ治疗后患者ＲＢＣ－Ｃ３ｂＲＲＲ明显升高,而ＲＢＣ－ＩＣＲＲ显著降低（Ｐ＜０．０１）。结论在０．２ＭＰａ下吸纯氧的ＨＢＯ辅助治疗能显著提高急性颅脑损伤患者红细胞免疫功能。     

不同海拔高度健康青年红细胞免疫功能的变化

本文应用酵母花环法检测了四个不同海拔高度107人健康青年男性的红细胞免疫粘附功能,同时测定了血清中的循环免疫复合物含量。结果表明:随海拔的升高,红细胞C_3b受体花环则降低,呈负相关性(P<0.01),红细胞免疫复合物花环和血清中循环免疫复合物则升高,呈正相关性(P<0.01)。红细胞C_3b受体花环与循环免疫复合物呈负相关性(P<0.01),红细胞免疫复合物花环与循环免疫复合物呈正相关性(P<0.01)。提示高原人体红细胞免疫功能的变化与空气中氧含量及辐射强度有关,因而在防治高原病时,注意调整和增强红细胞免疫功能具有十分重要的意义。     

Eryptosis and hemorheological responses to maximal exercise in athletes: Comparison between running and cycling

We compared the effects of cycling and running exercise on hemorheological and hematological properties, as well as eryptosis markers. Seven endurance‐trained subjects randomly performed a progressive and maximal exercise test on a cycle ergometer and a treadmill. Blood was sampled at rest and at the end of the exercise to analyze hematological and blood rheological parameters including hematocrit (Hct), red blood cell (RBC ) deformability, aggregation, and blood viscosity. Hemoglobin saturation (SpO2), blood lactate, and glucose levels were also monitored. Red blood cell oxidative stress, calcium content, and phosphatidylserine exposure were determined by flow cytometry to assess eryptosis level. Cycling exercise increased blood viscosity and RBC aggregation whereas it had no significant effect on RBC deformability. In contrast, blood viscosity remained unchanged and RBC deformability increased with running. The increase in Hct, lactate, and glucose concentrations and the loss of weight at the end of exercise were not different between running and cycling. Eryptosis markers were not affected by exercise. A significant drop in SpO2 was noted during running but not during cycling. Our study showed that a progressive and maximal exercise test conducted on a cycle ergometer increased blood viscosity while the same test conducted on a treadmill did not change this parameter because of different RBC rheological behavior between the 2 tests. We also demonstrated that a short maximal exercise does not alter RBC physiology in trained athletes. We suspect that exercise‐induced hypoxemia occurring during running could be at the origin of the RBC rheological behavior differences with cycling.     

黄芪对严重烧伤病人红细胞免疫功能的影响

目的：研究黄芪对严重烧伤病人红细胞免疫功能的影响。方法：将30名烧伤面积为30％以上的烧伤病人,随机分为烧伤对照组和黄芪治疗组。黄芪治疗组患者给予黄芪精口服液,一日3次,每次10ml,连服35天。两组患者伤后每隔7天检测一次红细胞C3b受体花环率（C3b花环率）,共检测5次。结果：烧伤对照组C3b花环率值明显低于正常（P＜0．01）,而黄芪治疗组C3b花环率值虽较正常为低,但于伤后两周左右始显著高于烧伤对照组（P＜0．01）。结论：黄芪对严重烧伤病人的红细胞免疫功能可发挥重要的调节作用。     

银屑病患者红细胞天然免疫功能的变化及临床意义

目的 研究银屑病患者红细胞天然免疫功能状况,并探讨其在银屑病发病机制中的作用。方法 测定新鲜血红细胞对肿瘤细胞的快速天然免疫反应,同时用流式细胞仪定量测定红细胞CD35。结果 银屑病患者肿瘤红细胞花环率和红细胞CD35分子定量均明显高于对照组(P<0.05),并且红细胞CD35分子定量与PASI评分是显著正相关(r=0.926,P<0.001)。结论 (1)银屑病患者红细胞天然免疫功能存在亢进和紊乱,红细胞CD35分子数量及其活性的增加是银屑病天然免疫反应能力亢进的基础,并且在银屑病免疫发病机制中可能起重要作用;(2)银屑病患者红细胞天然免疫功能的测定可作为判断银屑病病情的客观指标之一。     

Resting cellular and physiological effects of freewheel running

INTRODUCTION/PURPOSE: Exercise modulates many aspects of physiology. The purpose of the current experiment was to characterize the impact of regular, moderate physical activity on resting, baseline measures of cellular immunity blood lipids, and muscle enzyme. METHODS: Male Fischer 344 rats were housed with either mobile (run, N = 10) or immobile (sedentary, N = 10) running wheels. After 4 wk of running, rats were sacrificed. Blood and muscle (long and medial heads of the triceps) were collected. From blood, white blood cell (WBC) differentials, red blood cell (RBC) count, hemoglobin, hematocrit, and lipid profiles were measured. Muscle citrate synthase (CS) activity was measured by spectrophotometric analysis. RESULTS: Rats ran an average of 9.89 +/- 0.79 km.wk(-1). There were no differences in the total number of circulating WBC, RBC, or eosinophils. Freewheel running decreased the number of circulating neutrophils (P < 0.001), monocytes (P < 0.01), and basophils (P < 0.01), while increasing the number of lymphocytes (P < 0.001), when compared with sedentary animals. Mean corpuscular content of hemoglobin was elevated in the freewheel group (P < 0.01). Physically active animals had slightly lower triglycerides and LDL, and elevated HDL. These changes resulted in a significant improvement in LDL/HDL ratio (P < 0.05). Muscle CS activity was unchanged between groups. CONCLUSION: Both the alterations in the RBC hemoglobin and lipid proteins are positive health changes associated with exercise training. The impact of the alterations in WBC differentials remains unknown but could indicate a reduction in inflammatory load. In conclusion, freewheel running provides sufficient exercise stimulus to produce some, but not all, training associated physiological adaptations.     

益欣康泰胶囊对高原红细胞增多症患者红细胞免疫功能影响

目的：为了观察益欣康泰胶囊对高原红细胞增多症患者和高原健康人的红细胞免疫功能的影响,我们对２１例高原红细胞２增多症患者和２４例健康人做了红细胞免疫功能服药前和服药后的比较,方法;采用红细胞Ｃ３ｂ受体花环率和免疫复合物花环率试验,结果显示：高原红细胞增多症患者和健康人服药后红细胞Ｃ３ｂ受体花环率服药后明显升高于服药前,经统计学分析有显著性差异,而免疫复合物花环率试验明显降低２,与服药前比较Ｐ〈０．０     

运动、锌铜营养与自由基代谢——Ⅳ.运动员体内的自由基水平及其与锌铜营养状态的关系

本实验分析了国家优秀运动员及一般大学生运动员的血浆及红细胞丙二醛(MDA)含量、红细胞超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活力,并对大学生运动员的血清锌、铜水平同时进行了测定。结果提示,优秀运动员的红细胞MDA含量显著低于大学生运动员;红细胞SOD、GSH-Px活力则显著高于大学生运动员。大学生运动员的红细胞SOD活力与其红细胞锌、血浆及红细胞铜水平均呈显著正相关。     

Hematological indices and iron status in athletes of various sports and performances

PURPOSE: Alterations of the red blood cell system and iron metabolism can influence physical performance. On the other hand, exercise can influence hematological variables. The purpose of this epidemiological study was to investigate the characteristics of the red blood cell system and the iron metabolism in athletes of different sporting disciplines and at different levels of performance. METHODS: We studied 851 male subjects (747 athletes, 104 untrained controls). Hemoglobin (Hb), hematocrit (Hct), red blood cell count (RBC), iron, transferrin, ferritin (Fer), and haptoglobin were analyzed in standardized blood samples, obtained after 2 d of rest, considering levels of performance (internationally, nationally, locally competitive, and leisure time), distinctive sporting category (endurance- (END), strength- (POW), and mixed-trained (MIX)), and, within endurance athletes, distinctive disciplines (cycling (CYC) and running (RUN)). RESULTS: No difference was found between athletes and controls in Hb and Hct. Reduced Hb, Hct, and RBC levels were observed in END compared with POW and MIX. These findings can mainly be attributed to exercise-induced plasma volume expansion, and only to a lesser degree and in selected athlete populations to hemolysis, as low haptoglobin is only observed in RUN, not in CYC, suggesting that not exercise itself but the "traumatic" movement of running might trigger the destruction of red blood cells. Physical activity of increasing duration and workloads (leisure time compared with competitive athletes) leads to decreased Fer levels in athletes, disregarding their discipline, but more pronounced in RUN. CONCLUSION: Physical training itself has no significant effect on selected hematological variables in athletes compared with untrained controls. The specific type and duration of exercise is of major importance in the adaptations of the blood cell system and the iron metabolism.