共查询到18条相似文献,搜索用时 125 毫秒
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目的:评价盐裂皮肤间接免疫荧光(IIF-SSS)在大疱性类天疱疮(BP)诊断中的价值。方法:采用单中心临床回顾性研究。纳入2013年1月至2019年1月在中国医学科学院皮肤病医院就诊的初诊BP患者163例,对照组404例,包括天疱疮161例、湿疹67例、药疹26例、多形红斑23例、结节性痒疹18例等。于患者用药前采血,... 相似文献
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盐裂皮肤直接和间接免疫荧光联合应用鉴别诊断表皮下大疱病的研究 总被引:1,自引:0,他引:1
本文探讨盐裂皮肤DIF和盐裂皮肤IIF联合应用鉴别诊断表皮下大疱病的价值。20例两种盐裂皮肤免疫荧光均阳性的病例比较表明,盐裂皮肤IIF示抗基底膜带抗体结合表皮侧或真皮侧与盐裂皮肤DIF示免疫反应物(IgG、IgA、C_3等)沉积于表皮例或真皮侧的部位基本一致。提示多数情况下盐裂皮肤DIF或IIF均可单独用于表皮下大疱病诊断,两者对比分析可以提高诊断率。 相似文献
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本文探讨盐裂皮肤DIF和盐裂皮肤IIF联合应用鉴别诊断表皮下大疱病的价值。20例两种盐裂皮肤免疫荧光均阳性的病例比较表明,盐裂皮肤IIF示抗基底膜带体结合表皮侧或真皮侧与盐裂皮肤DIF示免疫反应物(IgG、IgA、C3等)沉积于表皮侧或真皮侧的部位基本一致。提示多数情况下盐裂皮肤DIF或IIF均可韵用于表皮下大将民病诊断,两者对比可以提高诊断率。 相似文献
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目的回顾性观察天疱疮、大疱性类天疱疮直接免疫荧光检查(DIF)结果,分析及评估其实验方法。方法活检取材,冰冻切片,DIF染色,荧光显微镜观察。结果免疫病理确诊143例,其中寻常型天疱疮(PV)阳性75例(52.4%)、红斑型天疱疮(PE)阳性13例(9.1%)、大疱性类天疱疮(BP)阳性53例(37.1%)、副肿瘤天疱疮(PNP)2例(1.4%)。临床与免疫病理诊断的符合率PV为65.3%(49/75),PE为61.5%(8/13),BP为69.8%(37/53),三者比较差异无统计学意义(P>0.05)。结论DIF对常见大疱性皮肤病的诊断是一项重要的、快速而特异性较高的检查方法,但仍需紧密结合临床确诊。 相似文献
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Scabies is an infestation caused by Sarcoptes scabiei and characterised by polymorphous lesions that may include burrows, papules, nodules, excoriation and crusts. Vesicular and bullous lesions are rather rare. Several diseases may be confused with scabies. We report a case of bullous scabies which, on the basis of the clinical and histopathological picture, mimicked bullous pemphigoid. Direct and indirect immunofluorescence were negative. Bullae recurred and persisted despite systemic corticosteroids. The patient was successfully treated with 5% permethrin and remained disease free for up to 12 months of follow-up. 相似文献
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We report the case of a 71-year-old Japanese woman who presented with persistent band-like erosions in the lower legs. Histological examination suggested subepidermal blister formation. Direct and indirect immunofluorescence studies revealed tissue-deposited and circulating immunoglobulin G autoantibodies against the basement membrane. Western blotting revealed autoantibodies to BP230, but not to BP180. Based on these findings, the patient was diagnosed as having a localized type of bullous pemphigoid. We suggest that the unique clinical manifestations in this patient could be attributable to bacterial or fungal infection, and/or mechanical trauma, such as the pressure of her socks. 相似文献
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Satyapal S Amladi S Jerajani HR 《Indian journal of dermatology, venereology and leprology》2002,68(6):330-333
Recent studies suggest that salt split skin is a more sensitive substrate than intact skin for immunofluorescence diagnosis of bullous pemphigoid. We undertook this study to define the role of salt split technique of immunofluorescence findings in 32 clinical and histopathology confirmed cases of bullous pemphigoid. Both direct and indirect immunofluorescences were performed using normal and split skin. Direct immunofluorescence positivity of 100% was noted with both routine and salt split method. Additional immunoreactant deposition was noted with direct method on split skin in 5 cases. Patterns of fluorescence in the latter were roof (40.60%), floor (9.4%) and combined roof and floor (50%). On indirect immunofluorescence, positivity was almost doubled with salt split technique ( 68%) as compared to routine method (36%). Thus, salt split technique was equivalent to routine on direct method in positivity with additional immunoreactant deposits noted in some and had double the sensitivity of the indirect method in detecting immunofluorescence in bullous pemphigoid. 相似文献
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【摘要】 目的 评价以正常人皮肤、猴食管及盐裂皮肤为底物的间接免疫荧光对自身免疫性表皮下水疱病的诊断价值。方法 选取2015年1月至2016年12月在中国医学科学院皮肤病研究所诊断的自身免疫性表皮下水疱病56例,其中大疱性类天疱疮(BP)47例,获得性大疱性表皮松解症(EBA)6例,线状IgA大疱性皮病2例,P200类天疱疮1例。对照组为70例天疱疮、15例慢性湿疹和15例健康成人。分别以正常人皮肤、猴食管及盐裂皮肤为底物行间接免疫荧光,观察荧光沉积情况,比较不同表皮下水疱病间接免疫荧光检测的敏感性和特异性。采用SPSS 13.0软件,计数资料比较采用χ2检验。结果 BP患者血清以正常人皮肤、猴食管为底物间接免疫荧光可见到荧光物质沿基底膜带线性沉积,盐裂皮肤间接免疫荧光可见BP患者荧光线性沉积于表皮侧,EBA和P200类天疱疮线性沉积于真皮侧。以正常人皮肤、猴食管及盐裂皮肤为底物间接免疫荧光对表皮下水疱病诊断的敏感性分别为73.2%、60.7%、94.6%,差异有统计学意义(χ2 = 18.2,P < 0.05),特异性分别为98.0%、100%、97.1%,差异无统计学意义(P > 0.05),以盐裂皮肤为底物时诊断的敏感性高于以正常人皮肤、猴食管为底物(χ2值分别为8.0、16.7,均P < 0.05)。结论 对于自身免疫性表皮下水疱病的诊断,盐裂皮肤为底物行间接免疫荧光检查优于以猴食管和正常人皮肤为底物。 相似文献
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药物相关性大疱性类天疱疮(drug-associated bullous pemphigoid, DABP)特指应用特定药物诱发的一种特殊类型的类天疱疮,其临床表现、组织学或免疫病理特征与特发性大疱性类天疱疮相同或相近。近年由药物导致大疱性类天疱疮的报道增多,本文就DABP的研究进展进行综述。 相似文献
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Laffitte E Favre B Fontao L Riou S Jaunin F Tamm K Saurat JH Borradori L 《The British journal of dermatology》2001,144(1):136-138
BACKGROUND: Bullous pemphigoid (BP) is a blistering disease associated with autoantibodies directed against two components of hemidesmosomes, BP180 and BP230. OBJECTIVES: To assess whether BP patients have autoantibodies targeting plectin, another hemidesmosomal component showing extensive homology to BP230. METHODS: Examination of sera from 16 patients with BP, using immunoprecipitation studies followed by immunoblotting. RESULTS: Serum of one of the 16 (6%) patients with BP contain autoantibodies binding to plectin, while no reactivity was found with sera from three control subjects. Sera from all 16 BP patients immunoprecipitated BP230 from extracts of biosynthetically radiolabelled human keratinocytes. CONCLUSIONS: Our results indicate that sera from BP patients might contain autoantibodies binding to plectin. Although this protein and BP230 are closely sequence-related, the occurrence of autoantibodies binding to plectin is a rare phenomenon in BP. 相似文献