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1.
肝细胞刺激物质对感染鸭乙型肝炎病毒的雏鸭体内抗病毒作用的实验研究 总被引:1,自引:0,他引:1
以感染鸭乙型肝炎病毒(DHBV)的北京雏鸭为体内实验动物模型,观察了肝细胞刺激物质(HSS)对鸭乙型肝炎病毒的抗病毒作用。1日龄北京鸭实验感染DHBV,7天后血清DHBVDNA阳性,给予药物治疗10天,用斑点杂交方法检测用药前后血清DHBVDNA,分析药物对血清DHBVDNA是否有抑制作用,结果显示:HSS大、小两个剂量组(分别为50mg/kg/d和200mg/kg/d)均对DHBVDNA有抑制作用,大剂量组出现DHBVDNA抑制作用时间早于小剂量组,用药5天、10天及停药3天血清DHBVDNA中位抑制率显著高于生理盐水对照组(P<0.05);两个剂量组均无停药反跳;生理盐水组用药前后DHBVDNA的A值比较差异无显著性意义(P>0.05)。停药后3天肝组织病理光镜及电镜均显示HSS组病变较生理盐水组轻。结论:肝细胞刺激物质有一定的保肝和抑制DHBVDNA复制的作用 相似文献
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目的探讨IgA肾病HBV感染与肾小管间质病变的关系。方法利用原位分子杂交(HBVDNA)、免疫组化(HBAg、CD3、CD8)以及HBVDNAHBAg和HBAgCD43双标记技术,对91例IgA肾病肾穿刺标本进行研究。结果肾组织内HBAg阳性率为69.2%。HBVDNA原位杂交阳性率为429%。HBVDNA阳性的病例,双重标记染色发现HBVDNA阳性的肾小管上皮细胞可表达HBcAg或/和HBsAg。HBV感染标记(HBVDNA、HBcAg、HBsAg)阳性组CD3阳性细胞和CD8阳性细胞数明显高于阴性组(P<001),并可见数量不等的T淋巴细胞入侵HBcAg及HBsAg阳性肾小管管壁或围绕其周围。结论感染HBV的肾组织细胞能够表达HBAg,并诱导CD3阳性细胞和CD8阳性细胞浸润,从而加重肾小管、间质损害。HBV感染对IgA肾病的发生发展可能起着重要作用 相似文献
3.
反义寡脱氧核苷酸在鸭体内抑制鸭乙型肝炎病毒复制与表达的研究 总被引:1,自引:0,他引:1
目的研究反义核酸的抗病毒作用。方法设计合成了针对鸭乙型肝炎病毒(DHBV)前S(PreS)基因区第951968位核苷酸的硫代反义寡脱氧核苷酸(ASODN),以20μg/g体重/日剂量对3只腹腔感染DHBV52毒株后,血清DHBsAg及DHBVDNA阳性鸭连续静脉注射10天,同时以等体积生理盐水注射另3只感染鸭作为对照。结果对照鸭注射生理盐水后,血清DHBsAg及DHBVDNA阳性未见明显改变,肝组织DNASouthern杂交在30与23kb左右杂交信号明显。注射ASODN鸭在10天后,2/3鸭血清DHBsAg及DHBVDNA量显著降低,3/3鸭肝组织中30kb左右的杂交信号显著减弱,23kb左右未见杂交信号。结论说明该段ASODN在鸭体内能部分抑制DHBV的复制与抗原表达。 相似文献
4.
鸭乙型肝炎病毒DNA体内转染的研究 总被引:2,自引:0,他引:2
利用两种鸭乙型肝炎病毒(DHBV)DNA双体质粒及环化的DHBVDNA体内转染2d龄鸭及4~20d龄鸭,大多数鸭产生了短暂病毒血症,少数鸭产持续病毒血症,转染鸭血清中检测得DHBs/preSAg,DHBVDNA及DHBV病毒颗粒,转染鸭血清腹腔注射1d龄鸭可感染成功,转染鸭肝组织检测到复制型DHBVDNA,提示转染后既有抗原有的表达,又有病毒的复制。另外,用两种DHBVDNA单体质粒体内转染2dx 相似文献
5.
反义寡脱氧核苷酸在鸭体内抑制鸭乙型肝炎病毒复制与 … 总被引:3,自引:0,他引:3
目的 研究反义核酸的抗病毒作用。方法 设计合成了针对鸭乙型肝炎病毒(DHBV)前S(PreS)基因区第951-968位核苷酸的硫代反义寡脱氧核苷酸(AS-ODN),以20μg/g体重/日剂量对3只腹腔感染DHBV5.2毒株后,血清DHBsAg及DHBV DNA阳性鸭连续静脉注射10天,同时以等体积生理盐水注射另3只感染鸭作为对照。结果 对照鸭注射生理盐水后,血清DHBsAg及DHBV DNA阳性未 相似文献
6.
肝细胞刺激物质对感染鸭乙型肝炎病毒的争论鸭体… 总被引:5,自引:0,他引:5
以感染鸭乙型肝炎病毒(DHBV)的北京雏鸭为体内实验动物模型,观察了肝细胞刺激物质(HSS)对鸭乙型肝炎病毒的抗病毒作用,1日龄北京鸭实验感染DHBV,7天后血清DHBVDNA阳性,给予药物治疗10天,用斑点杂交方法检测用药前后血清DHBVDNA,分析药物对血清DHBVDNA有抑制作用,大剂量量组出现DHBVDNA抑制作用时间早小剂量组,用药5天,10天及停药3天血清DHBVDNA中位抑制率显著高 相似文献
7.
应用原位杂交和免疫组化PAP法双标记技术,结合病人乙型肝炎(乙肝)病毒血清学标志物检测结果,研究了31例慢性乙肝病人肝穿刺组织中乙型肝炎病毒DAN和HBsAg的分布及意义。结果显示,肝辔内检HVDNA23例,HBsAg26例,二者同时检出者21例。从同组病人肝组织的HBVDNA和HBsAg双标检测结果与其乙肝病毒血清学标志物检测结果的比较来看,肝组织内HBVDNA和HBsAg同时很可能表明HBV正 相似文献
8.
核酶对鸭乙型肝炎病毒感染体内抗病毒作用效果的观察 总被引:2,自引:0,他引:2
目的观察核酶在体内抗病毒作用效果。方法选择鸭乙型肝炎(DHBVLJ-76)及其鸭动物模型作为检测系统,将针对DHBVPre-S736位点的核酶(RzDS)插入pJ120质粒构建成pJ-RzDS。与痘苗病毒天坛761株(VV)同源重组后获得含RzDS的重组痘苗病毒(V-RzDS)。用DHBV感染1日龄北京鸭,分别在感染的同时、感染后24小时和72小时注射V-RzDS和VV,10天后检测血清中DHBVDNA和DHBsAg的含量。结果北京鸭在感染DHBV的同时注射V-RzDS,其DHBVDNA和DHBsAg的含量均低于VV对照组,两组之间有显著性差异。结论提示RzDS对DHBV基因组复制和表达均有抑制作用,其抑制率分别为45%和63%。 相似文献
9.
乙肝张黄曲霉毒素B1诱发树Qu肝癌的病理变化 总被引:5,自引:0,他引:5
目的:对人乙型肝炎病毒(HBV)和黄曲霉毒素B1(AFB1)引起的树Qu肝脏病变进行动态比较观察。方法:成年树Qu按不同处理分为A(HBV+AFB1)、B(HBV)、C(AFB1)、D(空白对照)4组。全部动物定期肝活检,并于160周结束实验时处理所有存活者。各次活检及尸检肝组织均作常规病理组织学检查,部分同时作HBsAg及HBsAg免疫组织化学、HBVDNA原位杂交,以及PASD、网状纤维染色等 相似文献
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11.
Detection of hepadnaviral DMA in extrahepatic tissues of human and animal models of hepatitis B virus (HBV) has raised the question of whether virus replication in organs other than the liver could be targeted for the treatment of chronic hepatitis B. Since duck hepatitis B virus (DHBV) replication is dynamic in the liver, kidney, pancreas, and spleen of newly hatched ducklings infected in ovo, we used the duck model and the new antiherpesvirus agent, famciclovir (FCV), to determine whether antiviral effect of nucleoside analogues on DHBV replication is pluripotential. Day-old ducklings hatched from eggs laid by a DHBV-carrier duck were bled and administered FCV (25 mg/kg/bd) orally for periods of 1, 2, 3, 6, 9, and 12 days. Seventeen (17) hours after the last dose of each regimen the duckling(s) was bled and postmortem samples of liver, kidney, pancreas, and spleen were snap-frozen and stored at ?70°. Analysis of plasma samples of ducklings treated for 2 days and longer by dot-blot hybridisation showed that levels of DHBV DNA were reduced significantly compared to levels in samples collected before treatment begun. Southern blot hybridisation of tissue DNA corroborated these results and showed that DHBV DNA replicative intermediates in all the tissues examined were reduced to levels that reflected the amount of virus released into the blood of each treated duckling. It is concluded from these results that if antiviral agents could be transformed to active metabolites in any infected tissues including the liver, replication of hepadnaviruses would be inhibited. We also note that the ability of young ducklings to metabolise FCV to the parent compound, penciclovir, suggests that hatchlings could be used for screening antiviral compounds under development. © 1994 Wiley-Liss, Inc. 相似文献
12.
L F Hung A E Brumbaugh G Bhatia P L Marion P P Hung D W Norbeck J J Plattner W S Robinson 《Journal of medical virology》1991,35(3):180-186
The effects on duck hepatitis B virus (DHBV) replication of specific analogues of two classes of chemical compounds not previously tested against hepadnaviruses are described. One is erythromycin A-9-methyloxime (EMO) and other oxime derivatives of erythromycin A, and the other is purine nucleoside analogues (cyclobut A and cyclobut G) with cyclobutane rings. Viral replication was assessed by measuring serum levels of DHBV DNA in infected ducklings and DHBV DNA in infected primary duck hepatocyte cultures. Administration of EMO 15 mg/kg of body weight IM to infected ducklings resulted in a rapid fall in DHBV DNA levels during therapy and a return to pretreatment levels after EMO administration was stopped. There was local toxicity at injection sites with muscle necrosis in some animals. When 100 mg/kg EMO was administered by gastric tube no such viral response was observed. The difference in virus response to EMO 15mg/kg IM and 100 mg/kg by gastric tube was not due to failure to achieve comparable blood and tissue levels of EMO administered by the different routes. The results suggest an indirect effect dependent on IM injection of EMO rather than a direct antiviral effect of the compound. Administration of cyclobut G or cyclobut A at 70 mg/kg IM led to a rapid reduction of DHBV DNA to undetectable levels in serum, and in only 1 of 4 animals did DHBV DNA became detectable again within 10 days after stopping the drug.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
13.
Intraperitoneal inoculation of duck hepatitis B virus in three different dosages (9 x 10(7), 1.8 x 10(8), 9 x 10(8) DHBV particles) into 3 to 21 day-old Chinese ducklings provided from a DHBV free flock produced a persistent infection up to 93.3% in 60 animals. The serum and liver specimens of these ducklings were examined by DNA dot blot hybridization on the 30th day after inoculation. The results showed that: (1) examination of viral DNA in liver was more sensitive and reliable than estimation of the DNA in serum for detecting DHBV infection in inoculated ducklings; (2) the liver DHBV DNA level did not coincide with the degree of liver hepatitis induced; (3) 21-day-old Chinese ducklings were also susceptible to DHBV infection, the infection rate of this group was 100% (10/10). 相似文献
14.
目的 建立标准化的湖北麻鸭乙型肝炎病毒(DHBV)动物模型,并初步观察拉米呋啶(3TC)体内抗DHBV的作用.方法将收集的转染细胞培养上清液纯化后作为体内实验感染的标准毒种,经腹腔接种2日龄雏鸭,连续观察50 d,用Real-Time PCR检测血清中DHBV DNA出现和持续时间.药物组则于接种3 d后给予3TC,检测观察期间感染鸭血清中病毒含量的变化情况.结果雏鸭实验感染率达到87.5%;雏鸭在接种后第7天出现病毒血症,血清病毒含量于第11天达到高峰值,后逐步降低,但在观察期内仍维持较高水平.在3TC治疗期间,感染鸭体内病毒血症处于较低水平,被感染肝细胞的数量也明显减少,且无明显的毒性作用;在停药3 d后,病毒血症即出现反跳现象,此后又有上升趋势.结论通过接种转染细胞上清液成功建立湖北麻鸭乙型肝炎病毒动物模型,药物评估实验证明其良好的稳定性和实用性;该模型也为研究DHBV生物学特性提供了有力的手段. 相似文献
15.
The therapeutic potential of plant extracts of Phyllanthus amarus and Phyllanthus maderaspatensis for postexposure prophylaxis against infection by Hepadnaviruses was studied in ducklings infected by the duck hepatitis B virus (DHBV). Forty-four Pekin ducklings were inoculated intraperitoneally with DHBV at 24 hr posthatch. They were treated by intraperitoneal injection of Phyllanthus amarus (aqueous extract) (100 mg/kg body weight) or Phyllanthus mad eraspatensis (alcoholic extract) (100 mg/kg body weight) for a period of 4 weeks. Infected ducklings treated with saline served as controls. Weekly serum samples obtained before, during, and after treatment were analysed for the presence of DHBV DNA in serum by dot blot hybridisation using α 32P-labelled probes. Liver tissue was collected after killing the ducks at various time intervals and was studied for replicative status of the viral DNA and liver histopathology; 17 of 21 ducks were viraemic on completion of treatment with Phyllanthus amarus. At 16 week posttreatment follow-up four of seven animals remained viraemic. Similar results were obtained with Phyllanthus maderaspatensis. There was no alteration in DHBV replication in the liver. No toxicity was observed with this treatment. These observations suggest that Phyllanthus amarus and Phyllanthus maderaspatensis are not useful as therapeutic agents for postexposure prophylaxis against DHBV infection. © 1993 Wiley-Liss, Inc. 相似文献
16.
Antiviral strategies in chronic hepatitis B virus infection: I. Establishment of an in vitro system using the duck hepatitis B virus model 总被引:4,自引:0,他引:4
N Bishop G Civitico Y Y Wang K J Guo C Birch I Gust S Locarnini 《Journal of medical virology》1990,31(2):82-89
Primary duck hepatocyte (PDH) cultures were established from ducklings congenitally infected with the duck hepatitis B virus (DHBV), plated onto feeder cell layers of irradiated human embryonic lung fibroblasts, and observed for 2 to 3 weeks. This system permitted the survival of the PDH in a differentiated form free of fibroblastic overgrowth for at least 3 weeks. The hepatocytes were shown to contain all the replicative DNA intermediates found during DHBV replication as well as the DHBV structural proteins PRE-S1, PRE-S2, and S of duck hepatitis B surface antigen (DHBsAg). The pool of supercoiled (SC) DHBV DNA increased dramatically from days 10 to 14 postplating. This PDH-feeder cell layer cell culture model provides a convenient system to study the effects of conventional inhibitors of DHBV replication and compounds targeted at the supercoiled form of DHBV DNA. This approach should allow the evaluation of a variety of strategies for treating chronic carriers of hepadnaviruses. 相似文献
17.
Coinfection of avian hosts by duck hepatitis B virus (DHBV) and reticuloendotheliosis virus (REV) was studied to assess the effect of immunodepression by REV on the replication of DHBV. One-day-old ducklings, domestic chickens, and turkey poults were inoculated either with DHBV or DHBV and REV and were bled and weighed at regular intervals. DHBV infection as manifested by viraemia and DHBV DNA in liver was established only in ducklings. All chickens and turkeys were negative for DHBV DNA in serum and liver. However, ducklings coinfected with REV showed a delayed onset and reduced level of viraemia compared to ducklings infected only with DHBV. The narrow host range of DHBV was confirmed even in immunodepressed species. It is suggested that the reduction in DHBV viraemia in ducklings was due to factors not involving the specific immune system. 相似文献
18.
K Vickery J S Freiman R J Dixon R Kearney S Murray Y E Cossart 《Journal of medical virology》1989,28(4):231-236
The immune response to duck hepatitis B virus (DHBV) had not been elucidated. An assay was therefore established to detect the presence of antibody to DHB surface antigen (anti-DHBs) in serum of experimentally inoculated and naturally infected ducks. Anti-DHBs in serum was detected by indirect RIA from the percentage inhibition of binding of rabbit anti-DHBs to purified DHBsAg. Specificity was confirmed by positive and negative controls, infected and noninfected sera, and a mouse monoclonal antibody to DHB core antigen (anti-DHBc). Serum and liver samples were tested for DHBV DNA by dot-blot hybridization assay. Adult ducks repeatedly inoculated with DHBV remained non-viraemic but developed anti-DHBs. This antibody activity neutralized the infectivity of DHBV, which was experimentally inoculated into 1-day-old ducklings. In naturally infected flocks anti-DHBs was detected in a proportion of noninfected adult ducks as well as 1-day-old hatchlings. Anti-DHBs activity in hatchlings neutralized the infectivity of experimentally inoculated DHBV. Pekin ducks can therefore mount a neutralizing antibody response to DHBV, and immunity may be transferred in ovo from dam to off-spring. 相似文献
19.
J Z Niu Y Y Wang M Qiao E Gowans P Edwards S P Thyagarajan I Gust S Locarnini 《Journal of medical virology》1990,32(4):212-218
Nine ducks congenitally infected with the duck hepatitis B virus (DHBV) were treated either orally (four ducks for 10 weeks) or intraperitoneally (five ducks for 12 weeks) with the Indian traditional herbal remedy Phyllanthus amarus. Compared to placebo-treated control ducks, these treatments did not result in a reduction of circulating viral DNA in the serum or in the level of viral DNA replication in the liver. In two of the five intraperitoneal-treated ducks, a reduction in the levels of duck hepatitis B surface antigenaemia (DHBsAg) was observed. The data strongly suggest that Phyllanthus amarus has no significant inhibitory effect on DHBV DNA replication and only a minor effect on DHBsAg production. 相似文献