HPLC法测定多潘立酮片含量及含量均匀度

目的建立HPLC法测定多潘立酮片的含量及含量均匀度。方法采用C18柱(4.6mm×250mm,5μm),流动相:甲醇-乙腈-0.5%醋酸铵溶液(55∶10∶35),检测波长:287nm,流速:1.0mL.min-1,柱温:30℃。结果测得多潘立酮线性范围为23.5～235.2μg.mL-1(r=0.9993),平均回收率为100.57%,RSD为1.86%。结论该法专属性强,操作简便,结果准确。     

反相高效液相色谱法测定多潘立酮片的含量

目的:探讨多潘立酮片的含量测定方法.方法:采用反相高效液相色谱法 (RP- HPLC法 ),色谱柱为 KromasilTMC18柱 (4.6 mm× 250 mm, 5 μ m),以甲醇- 0.5%醋酸铵溶液 (70 : 30)为流动相,流速为 1.0 mL/min,柱温为 35℃,检测波长为 285 nm.结果:多潘立酮在 10. 7～ 214. 0 μ g/mL范围内浓度与峰面积具有良好的线性关系, r=0.999 6,回收率为 99.5%, RSD为 0.68% (n=5).结论: RP- HPLC法可用于多潘立酮片的含量测定.     

UPLC-MS/MS法测定多潘立酮口腔崩解片的含量

目的采用超高效液质联用方法测定多潘立酮口腔崩解片的含量。方法色谱柱:ACQUITY UPLC BEH C_(18)(2.1 mm×50 mm,1.7μm),流动相:甲醇-2 mmol/L乙酸铵水溶液(80∶20),流速:200μL/min;柱温:35℃,质谱采用多离子反应监测模式(MRM),选择正离子模式检测。结果多潘立酮在0.006 2~6.2μg/mL范围内与峰面积呈良好的线性关系(r=0.996 3,n=6),最低定量限为6.2 ng/mL。加样回收率为100.23%(n=6),RSD为1.78%。结论本方法灵敏度高、专属性强、结果准确,可用于多潘立酮口腔崩解片的质量控制。     

HPLC法测定布洛芬片的含量

目的:建立HPLC法测定布洛芬片的含量。方法:采用Agilent C_8柱(150mm×4.6mm,5μm),以乙腈-0.01mol·L~(-1)磷酸(40:60)为流动相,流速为1.0ml·min~(-1),检测波长:220nm。结果:布洛芬在200～1400μg·ml~(-1)的浓度范围内线性关系良好,r=0.9998(n=7),平均回收率为99.90%(n=9),RSD为0.63%。结论:该方法专属性强,操作简便,结果准确。     

反相高效液相色谱法测定双嘧达莫片的含量

目的 建立双嘧达莫片的反相高效液相色谱含量测定方法,更有效地控制药品质量.方法 采用Diamonsal C18柱(150mm×4.6mm,5μm),流动相为0.05% 磷酸二氢钾-甲醇(20:80),流速1.5mL·min-1,检测波长为283nm.结果 双嘧达莫在6.25～250/μg·mL-1范围内线性关系良好,r=1(n=11),平均回收率为99.17%,RSD为0.49%(n=6).结论 该方法简便、快速、准确.     

HPLC法测定多潘立酮片的含量及含量均匀度

目的:建立高效液相色谱法测定多潘立酮片中多潘立酮的含量及含量均匀度的方法。方法:色谱柱为KromasilC18(250mmX4.6mm,5μm),流动相为甲醇-水(3:1),检测波长为287nm,流速为1ml.min-1。结果:多潘立酮的平均回收率为100.03%,RSD为0.75%(n=5)。本法与标准法比较,没有显著性差别。结果:方法简便,结果准确,专属性强,可用于含量及含量均匀度的测定。     

HPLC法测定他米巴罗汀片的含量

目的 建立HPLC法测定他米巴罗汀片的含量.方法 色谱柱Eclipse XDB-C18(4.6mm×150mm,5μm),流动相:乙腈-0.2%磷酸溶液(58:42),流速为1.0mL·min-1,检测波长:234nm.结果 样品在1.04~20.8μg·mL-1范围内呈现良好的线性关系(r=1,n=7),平均回收率为100.9%,RSD为1.1%(n=9).结论 该方法简便,快速,准确.     

高效液相色谱法测定四环素片的含量

目的建立高效液相色谱法测定四环素片含量。方法色谱柱为SUPELCO Discovery C18(250mm×4.6mm,5μm),流动相为0.1%磷酸-甲醇(40:60),检测波长为268nm,柱温为30℃,流速1.0ml/min。结果四环素进样量的线性范围为0.1～8.0μg,r=0.9983(n=7),平均回收率为99.92%,RSD=0.89%(n=6)。结论所用方法准确、灵敏、专属性好,可用于四环素片的质量控制。     

HPLC法测定丙谷胺片中丙谷胺含量

目的建立测定丙谷胺片含量的方法。方法采用HPLC法,色谱柱为HypersilOSD C18(250×4.0mm,5μm),流动相为乙腈∶pH 7.2的磷酸盐缓冲液(30∶70),测定波长224nm。结果线性范围12.64~63.20μg.mL-1,r=1;平均加样回收率为99.9%,RSD=0.5%(n=6)。结论方法简便、重现性好,准确可靠。     

HPLC法测定地西泮片的含量及含量均匀度

目的建立测定地西泮片的含量及含量均匀度的HPLC法。方法 C18柱(150mm×4.6mm,5μm),以甲醇-水(70:30)为流动相,流速1mL.min-1,检测波长254nm,柱温为30℃。结果地西泮在0.05240～0.5240g.L-1的质量浓度范围内线性关系良好(r=0.9998,n=6),平均加样回收率为100.1%,RSD为0.3%。结论该方法简便,准确,专属性强。     

Antioxidant,anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®

Context: Polyherbal formulations containing different plants are used for the treatment of various diseases. Romix^® powder is a polyherbal formulation consisting of 14 traditionally used herbs and is used as a food supplement. There is no information about pharmaceutical activities of Romix^®.

Objective: This study determined the total phenolic and total flavonoid content, and investigated the antioxidant and anti-inflammatory activities and acute toxicity of Romix^®.

Material and methods: The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteu reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The quercetin content of the extract was analyzed using the high-performance liquid chromatography (HPLC) method. Antioxidant activity of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assays. The anti-inflammatory activity was evaluated by the carrageenan-induced paw edema test in the rat.

Results: The flavonoid and phenolics contents of Romix^® were 50.58 and 265.83?mg/g in ethanol extract and 18.60 and 222.50?mg/g in water extract, respectively. Total quercetin content of Romix^® was determined as 2.857?mg/g. Antioxidant activity results showed that ethanol extract in 1?mg/mL concentration (4.49775 µg/mL) had moderate antioxidant activity than water extract in the same concentration (4.28191 µg/mL). Intraperitoneal administration of 25?mg/kg Romix^® extract exhibited anti-inflammatory activity and inhibited paw swelling at 1, 2, 3, 4, 5 and 6?h in rats with no acute toxicity.

Conclusion: These findings suggest that Romix^® due to its antioxidant and anti-inflammatory activities may be useful in the prevention or treatment of aging-related and inflammatory diseases.     

Effects of a central stimulant substance isolated from the sea anemone Stoichactis kenti on brain monoamines of the mouse

An active substance, polypeptide in chemical nature, causing central stimulant activity in mice has been isolated from a sea anemone. Signs of central nervous stimulatory activity produced by this substance included fighting episodes, increased motor activity and clonic convulsions. The active substance produced significant decreases in brain norepinephrine concentration both at the ED₅₀ (6.4 mg/kg) and the maximum effective dose (9.3 mg/kg) during the stimulation period. Brain dopamine concentration was significantly decreased by the active substance at the ED₅₀ dose whereas brain serotonin concentration was not affected. The ED₅₀ dose of the active substance significantly inhibited the re-uptake of norepinephrine during the stimulation period and elevated hormetanephrine levels. These results suggest that the active substance causes central stimulation by releasing active norepinephrine from functional pools and inhibiting its re-uptake, thus making more norepinephrine available at a adrenoceptors. It is probable that brain dopamine plays a minor role in this stimulant action of the active substance.     

高效液相色谱法测定舒必利片的含量及含量均匀度

目的建立测定舒必利片的含量及含量均匀度的高效液相色谱法。方法色谱柱为Phenomenex Kromasil C18柱（250mm×4．6mm,5μm,）流动相为乙睛-水（8：92,用冰醋酸调pH至3．6）,检测波长为253nm,流速为1．0mL／min。结果 舒必利进样量线性范围为2～14μg,r=0．9998（n=7）,平均回收率为99．70％,RSD为0．71％（n=6）。结论所用方法简便准确、灵敏度高、重现性好,适用于舒必利片的质量控制。     

高效液相色谱法测定米非司酮片的含量和含量均匀度

目的建立高效液相色谱法测定米非司酮片的含量和含量均匀度的方法。方法采用Zorbax XDB C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-水-三乙胺(75∶25∶0.05),流速1.0mL·min-1,检测波长为306nm。结果米非司酮在1.0979～43.916μg·mL-1(r=0.9999)范围内具有良好的线性,回收率为100.03%,RSD为0.22%。结论该方法操作简便,结果准确,重现性好,可用于米非司酮片的质量控制。     

HPLC测定硫酸阿托品片的含量及含量均匀度

目的 建立高效液相色谱法测定硫酸阿托品片的含量及含量均匀度。方法 采用SHISEIDO CAPCELL C_{18(250 mm×4.6 mm,5 μm)色谱柱,以0.01 mol·L^-1磷酸二氢钾溶液(含0.15%三乙胺,用磷酸调节pH值至6.0)-甲醇(75∶25)为流动相,流速：1.0 mL·min^-1,检测波长：210 nm,柱温：35 ℃,进样量：20 μL。结果 硫酸阿托品浓度在5.878 8~ 58.788 μg·mL^-1内线性关系良好,回归方程为Y=24.015 8X+0.095 9,r=1.000 0,平均回收率为100.2%(n=9)。结论 该方法简便快速,可用于硫酸阿托品片含量和含量均匀度的测定。}     

HPLC测定利胆合剂中白藜芦醇含量

目的建立测定秋水仙碱片含量及含量均匀度的方法。方法采用C8柱,甲醇-水(40∶60)为流动相,检测波长为254 nm。结果线性范围为2~20μg.mL-1(r=0.999 9),平均回收率为100.2%(RSD=0.6%,n=9)。结论本法用于测定秋水仙碱片的含量和含量均匀度,结果准确灵敏,可获得满意的测定结果。     

艾司唑仑片含量及含量均匀度测定方法探讨

目的建立HPLC法测定艾司唑仑的含量及含量均匀度。方法采用ALLTECH Kcromaisil-C18谱柱(250mm×4.6mm,5μm),以甲醇-水(70∶30)为流动相,检测波长233nm,流速:0.8mL.min-1,柱温:30℃,进样量:10μL。结果艾司唑仑线性范围为10.48~104.8μg.mL-1(r=0.9998),平均回收率为100.4%(RSD=1.6%)。结论本方法灵敏度高,操作简便、可靠,适用于艾司唑仑片的质量控制。     

高效液相色谱法测定酮咯酸氨丁三醇胶囊含量及含量均匀度

目的建立反相高效液相色谱法测定酮咯酸氨丁三醇胶囊含量及含量均匀度。方法采用Hypersil ODS C18为分析柱(4.6 mm×250 mm,5μm),流动相为甲醇-水-冰醋酸(55∶44∶1),流速为1.0 mL.min-1。检测波长249 nm。结果酮咯酸氨丁三醇线性范围为0.127 8~0.760 8 mg.mL-1,r=0.999 8,回收率为99.3%,RSD为0.24%。结论该方法简便,准确,专属性强,可用于该胶囊质量控制研究。     

碘酸钾片干法制片工艺研究

采用滚压法制粒工艺制备碘酸钾片，并对其含量及含量均匀度进行了研究。