阿托伐他汀对血脂正常原发性高血压患者血管内皮功能及血管活性物质的影响

目的 探讨阿托伐他汀对血脂正常的原发性高血压(EH)患者血管内皮依赖性舒张功能(FMD)及血管活性物质的影响.方法 血脂正常的EH患者65例随机分为阿托伐他汀组33例,常规治疗组32例,另选取25名门诊健康体检者作为健康对照组.阿托伐他汀组在常规降压治疗基础上加入阿托伐他汀20 mg,每晚1次口服.应用无创超声检查技术观察用药前后肱动脉血流介导的FMD的变化,同时检测一氧化氮(NO)、内皮素(ET-1)及血管性假性血友病因子(vWF)的含量变化.结果 (1)治疗后阿托伐他汀组及常规治疗组FMD、NO水平均较治疗前明显上升,阿托伐他汀组与常规治疗组比较,FMD[(15.8±3.1)%与(8.7±2.5)%,t=4.048,P＜0.01]、NO[(49.9±5.6)μmol/L与(36.8±7.6)μmol/L,t=3.004,P＜0.01]显著高于常规治疗组.(2)治疗后阿托伐他汀组及常规治疗组ET-1及vWF均较治疗前明显降低,阿托伐他汀组与常规治疗组比较,ET-1[(43.5±13.7)ng/L与(52.5±14.7)ng/L,t=2.968,P＜0.01]、vWF[(124.7±28.5)%与(143.3±27.7)%,t=2.822,P＜0.01]显著低于常规治疗组.结论 在血脂正常的EH患者中,阿托伐他汀可通过提高血浆NO浓度和降低ET-1、vWF浓度等非调脂机制改善血管内皮功能.

Abstract：

Objective To investigate the effect of atorvastatin on vascular endothelial cell function and vasoactive substances in essential hypertensive patients without hyperlipemia. Methods Sixty-five essential hypertensive(EH) patients without hyperlipemia were enrolled and randomly divided into atorvastatin group and conventional treatment group(oral taken atorvastatin or placebo once every night in addition of routine antihypertensive drugs).Twenty five healthy subjects were also recruited as control.All cases were followed up for eight weeks.Serum cholesterol,nitric oxide(NO),emdothelin-1(ET-1),vonWillebrand-factor(vWF) levels were determined in each case.Flow-medizted dilation(FMD) was determined by high-resolution ultrasonography before and after eight weeks atorvastatin medication.Results (1)Before treatment,the FMD and NO levels of EH group were significantly lower than those of control group(P＜0.01),while the ET-1 and vWF levels of EH group were significantly higher than those of control group(P＜0.01);(2)In EH patients,the FMD and NO levels significantly increased after treatment and increased even more dramatically in atorvastatin group,when compared to conventional treatment group(Ps＜0.01);(3)In EH patients,the ET-1 and vWF levels significantly decreased after treatment and decreased even more dramatically in atorvastatin group,when compared to conventional treatment group(Ps＜0.01).Conclusion In patients of EH without hyperlipemia,atorvastatin can decrease plasma levels of ET-1,vWF,while increase plasma NO concentration and improve vascular endothelial function.     

伴有阻塞性睡眠呼吸暂停综合征的急性脑梗死患者血浆溶血磷脂含量的测定

Objective To observe the changing characteristics of plasma lysophosphatidic acid (LPA) or acidia phospholipid (AP) levels in patients with obstructive sleep apnea syndrome-associated(OSAS)acute cerebral infarction and to explore the pathophysiological mechanisms of OSAS-related stroke so as to provide basis for clinical antithrombotic therapy. Methods Thirty-six patients of OSAS, 32 patients of OSAS-related acute stoke and 36 patients of acute stoke without OSAS diagnosed by clinical and accessory examinations were enrolled in the current study. Thirty-eight age-matched healthy subjects were recruited as controls. The changes of the plasma LPA and AP levels were measured. Results Within 24 hours after symptom onset, the plasma LPA and AP levels in the OSAS-related acute cerebral infarction group (LPA(3. 78 ±0. 56) μmol/L; AP(7. 63 ± 1. 38) μmol/L) were significantly higher than those in the OSAS group(LPA(3. 17 ±0. 65) μmol/L; AP(6. 60 ± 1. 20) μmol/L) ,the not OSAS-related acute cerebral infarction group (LPA (3. 40 ± 0. 59)μmol/L; AP (6. 41 ± 1. 37)μmol/L) and the control group (LPA(2.76±0.45)μmol/L;AP(4.52±0. 83) μmol/L (P < 0. 01)) . The levels of LPA and AP in the OSAS group and the not OSAS-related acute cerebral infarction group were significantly higher than those in the control group(P<0. 01). Seven days after symptom onset, the plasma LPA and AP levels in the OSAS-associated acute cerebral infarction group (LPA(3.08 ± 0. 58) μmol/L; AP(6. 15 ±1. 14)μmol/L) were still higher(P < 0. 01) . The plasma LPA levels were not significantly different among the OSAS-related acute cerebral infarction group, the not OSAS-related acute cerebral infarction group and the control group 21 days after symptom onset, whereas the plasma AP levels in the OSAS-related acute cerebral infarction group (5. 04 ± 0. 83) μmol/L were still significantly higher than those in the not OSAS-related acute cerebral infarction group (4. 57 ± 0. 94) μmol/L and the control group (P < 0.05). Conclusions The significantly elevated plasma LPA and AP levels in patients with OSAS suggested that platelets in vivo are in an activated state and in cerebral ischemia and hypoxia state, especially for the OSAS-related acute cerebral infarction patients. The activated state of platelet may persist for a long time, thus the time window for antithrombotic therapy may be longer.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

The changes of plasma 8-iso-prostaglandin F2αand serum C-reactive protein levels in patients with obstructive sleep apnea-hypopnea syndrome

Objective To investigate the plasma 8-iso-prostaglandin F2α(8-iso-PGF2α and the serum C-re-active protein(CRP) levels in patients with obstructive sleep apnea-hypopnea syndrome(OSAHS) with and without hypertension(OSAHS + HT),and to explore the changes of pathophysiology in patients with OSAHS and the patho-genesis of OSAHS + HT. Methods All observed subjects were divided into 3 groups: control group (n=20),OS-AHS group(n=19),OSAHS + HT group (n=21). Plasma 8-iso-PGF2αand serum CRP concentrations levels were measured by ELISA and were compared. Results The plasma 8-iso-PGF2αand serum CRP levels,were higher in OSAHS patients than those in control subjects [(11.08±3.26)μg/L vs (7.49±2.10)μg/L,P<0.01;(1.75±0.82) mg/L vs (0.52±0.26 ) mg/L,P<0.01],and were higher in OSAHS + HT group than those in control group [14.84±3.43)μG/L vs(11.08±3.26)μg/L,P<0.01 ;(3.13±1.06)mg/L vs(1.75±0.82)mg/L,P<0.01]. Conclusions Oxidative stress and inflammation in OSAHS patients are increased,which are involved in the devel-opment of OSAHS associated hypertension.     

血浆同型半胱氨酸的表达及变化在不同病因大细胞贫血患者中的鉴别及治疗意义

Objective To examine the serum homocysteine,folic acid and Vitamin B12 levels in macrocytic anemia patients and observe their dynamic changes following therapy. Methods Homocysteine,folic acid and Vita-min B12 were analyzed by electro-chemiluminescence immunoassay. Complete blood cell count was analyzed by opti-cal method and resistance method. Results The homocysteine is significantly higher in nutritional megaloblastic a-nemia[ (71.26±27.84)μmoL/L ] than in drug-induced megaloblastic anemia[(11.44±5.06)μmol/L],in myel-odysplastic syndrome[ (9.51±4.13)μmol/L] and in the normal control group[(8.74±5.42)μmoL/L] (P<0.01). After treated with low-dosage folic acid and Vitamin B12,the patients with megaloblastic anemia presented slow declining but eventually normal homocysteine levels,compared with those received high-dosages. Conclusion Homocysteine can be used for differential diagnosis of macrocytic anemia. The duration of remaining of abnormal ho-mecysteine levels is related to the dosage of folic acid and Vitamin B12.     

血浆同型半胱氨酸的表达及变化在不同病因大细胞贫血患者中的鉴别及治疗意义

Objective To examine the serum homocysteine,folic acid and Vitamin B12 levels in macrocytic anemia patients and observe their dynamic changes following therapy. Methods Homocysteine,folic acid and Vita-min B12 were analyzed by electro-chemiluminescence immunoassay. Complete blood cell count was analyzed by opti-cal method and resistance method. Results The homocysteine is significantly higher in nutritional megaloblastic a-nemia[ (71.26±27.84)μmoL/L ] than in drug-induced megaloblastic anemia[(11.44±5.06)μmol/L],in myel-odysplastic syndrome[ (9.51±4.13)μmol/L] and in the normal control group[(8.74±5.42)μmoL/L] (P<0.01). After treated with low-dosage folic acid and Vitamin B12,the patients with megaloblastic anemia presented slow declining but eventually normal homocysteine levels,compared with those received high-dosages. Conclusion Homocysteine can be used for differential diagnosis of macrocytic anemia. The duration of remaining of abnormal ho-mecysteine levels is related to the dosage of folic acid and Vitamin B12.     

盐酸戊乙奎醚对脂多糖诱导的内皮细胞损伤的保护作用

目的 探讨盐酸戊乙奎醚(PHC)对脂多糖(LPS)诱导的内皮细胞损伤的影响及其作用机制.方法 用RPMI 1640培养基将人脐静脉内皮细胞在5%CO2、37℃培养箱中培养,并分为对照组,LPS组及PHC组.检测细胞上清液乳酸脱氢酶(LDH)、细胞中丙二醛(MDA)、超氧化物歧化酶(SOD)和一氧化氮(NO)水平.提取细胞核蛋白,Western blot分析细胞外信号调节激酶1/2(ERK1/2)和c-Jun氨基末端激酶(JNK)磷酸化蛋白的表达.采用SPSS 13.0统计软件进行分析,组间比较采用单因素方差分析,以P<0.05为差异具有统计学意义.结果 与对照组比较.LPS组LDH含量[(1 642±367)U/L vs.(169±33)U/L]、MDA含量[(13.2±1.2)nmol/L vs.(7.2±0.8)nmoL/mL]及NO水平[(143.2±10.3)μmol/L vs.(85.5 4.4.1)μmol/L]明显增多,SOD活性明显下降[(41.2 ±2.7)U/mL vs.(61.1±2.8)U/mL],ERK1/2和JNK磷酸化表达明显增高.PHC可显著降低LDH含量[(392 4.90)U/L],降低MDA[(8.6±1.3)nmol/mL]和NO水平[(92.1 ±6.6)μmol/L],提高SOD活性[(58.0±3.0)U/mL],减弱ERK1/2磷酸化蛋白表达.结论 盐酸戊乙奎醚对脂多糖诱导的内皮细胞损伤具有保护作用,其作用机制可能与抑制ERK1/2磷酸化表达减少过氧化物的产生有关.

Abstract：

Objective To investigate the effects of penehyclidine hydrochloride ( PHC) on lipopolysaccharide (LPS) -induced endothelial cells injury and its mechanism. Methods ECV-304 was cultured in RPMI1640 in a 5% humidified CO2 atmosphere at 37 ℃. Then cultured cells were used to assess the following treatments: control group, LPS group (1 μg/mL) and PHC group(2 μg/mL). At the end of the experiments, supernatant was collected for determination of lactate dehydrogenase ( LDH), and cells were collected for determination of malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) levels. And extracellular regulated kinasel/2( ERKl/2)and JNK MAPK (mitogen-activated protein kinases, MAPK) protein expressions were determined using Western blot technique. Analysis of variance (ANOVA) was used for statistical analysis to compare values among all groups. A significant difference was presumed for a probability value < 0.05. Results Compared with control group, LDH leakage [(1642 ± 367) U/L vs (169±33)U/L], the contents of MDA[(13. 2 ± 1. 2) nmol/L vs (7. 2 ±0. 8)nmol/mL] and NO levels [(143.2 ± 10.3) μmol/L vs(85.5 ±4.1) μmol/L], expressions of ERK1/2 and JNK were remarkably increased and SOD activities[(41.2 ±2.7) U/mL vs (61. 1 ±2.8) U/mL] were obviously decreased in LPS group. PHC markedly decreased LDH leakage [(392 ±90) U/L], MDA contents [(8. 6 ± 1. 3) nmol/ mL] and NO levels [(92.1 ±6.6) μmol/L], ERK1/2 activation and enhanced SOD activities [(58.0± 3.0) U/mL]. Conclusions PHC could protect endothelial cells against LPS-induced cell injury. The effect of PHC is likely mediated through inhibition of ERK1/2 MAPK activation.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

特发性肺纤维化患者血清肿瘤标志物含量变化及其临床意义

目的 探讨特发性肺纤维化(IPF)患者血清多种肿瘤标志物含量的变化及其临床意义.方法 采用化学发光法检测25例IPF患者(IPF组)和23例正常对照者(对照组)外周血多种肿瘤标志物甲胎蛋白(AFP)、癌胚抗原(CEA)、CA125、CA153、CA199,同时检测患者血气、肺功能参数等临床指标.结果 IPF组与对照组外周血AFP[(2.41±0.95)μg/L与(1.29±1.13)μg/L,t=4.32,P＜0.05]、CEA[(7.12±2.68)μg/L与(2.14±0.57)μg/L,t=3.69,P＜0.05]、CA125[(118.52±31.24)kU/L与(23.97±8.47)kU/L,t=4.15,P＜0.05]、CA153[(47.58±14.57)kU/L与(9.24±4.21)kU/L,t=5.87,P＜0.05]、CA199[(165.78±21.13)kU/L与(14.51±5.74)kU/L,t=4.22,P＜0.05]均明显高于对照组,差异具有统计学意义.且血清AFP、CEA、CA125、CA153、CA199含量均与动脉血氧分压(r值为-0.15～-0.55,P＜0.05)、肺活量(r值为-0.09～-0.49,P＜0.05或P＜0.01)、肺弥散量(r值为-0.17～-0.61,P＜0.05)呈负相关.各肿瘤标志物与ESR和CRP无相关性(P均＞0.05).血清多种肿瘤标志物的进行性升高与肺纤维化程度的进展正相关.经激素治疗后,25例IPF患者血清AFP[(2.41±0.95)μg/L与(1.67±1.22)μg/L,t=5.41,P＜0.05]、CEA[(7.12±2.68)μg/L与(3.75±1.96)μg/L,t=4.63,P＜0.05]、CA125[(118.52±31.24)kU/L与(53.22±13.56)kU/L,t=3.97,P＜0.05]、CA153[(47.58±14.57)kU/L与(19.35±8.74)kU/L,t=5.15,P＜0.05]、CA199[(165.78±21.13)kU/L与(58.85±17.31)kU/L,t=6.34,P＜0.05]水平明显下降.结论 IPF患者血清多种肿瘤标志物含量增加,并与肺功能损害程度密切相关,可能提示肺纤维化的进展,是预后不良的标志.

Abstract：

Objective To investigate the clinical significance of multi-tumor biomarkers combined variation in patients with idiopathic pulmonary fibrosis(IPF).Methods Multiple serum tumor biomarkers,including AFP,CEA,CA125,CA153 and CA199 were detected in 25 patients with IPF and 23 healthy controls by chemiluminescence method,and the blood gas analysis,pulmonary function parameters and other clinical data were also collected simultaneously.Results All serum biomarkers measured in this study were significantly higher in IPF group than control,AFP([2.41±0.95]μg/L vs.[1.29±0.13]μg/L,t=4.32,P＜0.05),CEA([7.12±2.68]μg/L vs.[2.14±0.57]μg/L,t=3.69,P＜0.05),CA125([118.52±31.64]kU/L vs.[23.97±8.47]kU/L,t=4.15,P＜0.05),CA153([47.58±14.57]kU/L vs.[9.24±4.21]kU/L,t=5.87,P＜0.05),and CA199([165.78±21.13]kU/L vs.[14.51±5.74]kU/L,t=4.22,P＜0.05).The serum levels of AFP,CEA,CA125,CA153,CA199 were negatively correlated with blood oxygen pressure(r=-0.15--0.55,Ps＜0.05),vital capacity(r=-0.09--0.49,Ps＜0.05),lung diffusion capacity(r=-0.17--0.61,Ps＜0.05),while no significant correlations were found between different biomarker levels and ESR and CRP(Ps＞ 0.05).In addition,the serum level of different tumor markers increased along with the progress of pulmonary fibrosis,which showed statistical significance(Ps＜0.05).After hormone medication therapy,the serum tumor marker levels decreased significantly in IPF patients[AFP[2.41±0.95]μg/L vs.[1.67±1.22]μg/L,t=5.41,P＜0.05,CEA([7.12±2.68]μg/L vs.[3.75±1.96]μg/L,t=4.63,P＜0.05,CA125([118.52±31.64]kU/L vs.[53.22±13.56]kU/L,t=3.97,P＜0.05),CA153(47.58±14.5 vs.19.35±8.74 kU/L,t=5.15,P＜0.05),CA199(165.78±21.13 vs.58.85±17.31 kU/L,t=6.34,P＜0.05)].Conclusion Multiple serum tumor biomarkers significantly increased in patients with IPF,and had a close correlation with lung function damage.The changes of these biomarkers indicated the progress of pulmonary fibrosis,which served as the predictors of poor prognosis.     

血管内皮生长因子与糖尿病视网膜病变关系的探讨

目的探讨糖尿病视网膜病变(DR)患者血管内生皮生长因子(VEGF)水平变化。方法用酶联免疫法测定68例糖尿病患者血浆VEGF水平,其中无视网膜病变组(NDR)30例、背景型视网膜病变组(BDR)28例、增殖型视网膜病变组(PDR)10例。正常对照组40例。结果糖尿病患者VEGF水平明显高于正常对照组(P<0.01),BDR组、PDR组明显高于NDR组(P<0.05)。结论VEGF水平与糖尿病视网膜病变的病变程度密切相关。定期测定糖尿病人血中VEGF可作为了解糖尿病视网膜病变程度的参考指标。     

糖尿病性视网膜病变患者一氧化氮变化的临床意义

目的　探讨一氧化氮 (NO)在糖尿病性视网膜病变中的意义。方法　检测糖尿病无视网膜病人 (NDR )、糖尿病视网膜变病人 (DR)及正常人血中NO的含量 ,进行对比分析。结果　NDR组NO含量显著高于正常对照组 (P <0 .0 1) ;DR组NO含量与正常对照组差异不显著 (P >0 .0 5 ) ,而明显低于NDR组 (P <0 .0 5 )。结论　NO参与了糖尿病性视网膜病变的发病机制 ;对糖尿病患者NO的深入研究 ,有望为糖尿病性视网膜病变的治疗开辟新的途径。     

同型半胱氨酸在糖尿病视网膜病变诊断中的应用

目的评价同型半胱氨酸（homocysteine,Hcy）测定在糖尿病视网膜病变（DR）诊断中的应用。方法应用循环酶法检测DR 45例、糖尿病无视网膜病变（NDR）50例及健康体检者40例血清Hcy含量,对结果进行统计分析。结果各组血清Hcy含量及高同型半胱氨酸（HHcy）发生率分别为：正常对照组（8.96±2.92）μmol/L、5.0%;NDR组（9.09±3.62）μmol/L、6.0%;DR组（21.82±7.99）μmol/L、73.3%;DR组HHcy血症发生情况及血清Hcy水平显著高于正常对照组及NDR组（P〈0.01）,而NDR组与正常对照组比较差异无统计学意义（P〉0.05）。结论血清Hcy水平升高也许是DR的危险因素之一,检测糖尿病患者血清Hcy水平有助于DR的早期诊断和治疗。     

血清及房水白细胞介素-6水平与糖尿病性视网膜病变的相关性

目的分析血清及房水中白细胞介素-6（interleukin-6,IL-6）水平与糖尿病性视网膜病变（diabetic retinopathy,DR）的相关性,探讨IL-6在DR发生、发展中的作用。方法60例2型糖尿病患者（实验组）,其中20例20眼无DR（non-DR,NDR）为NDR组,20例20眼非增生性DR（non-proliferative DR,NPDR）为NPDR组,20例20眼增生性DR（proliferative DR,PDR）为PDR组;健康老年性白内障患者20例20眼为对照组。采用电化学发光法检测各组血清及房水中IL-6水平,并进行比较。结果 NDR组、NPDR组、PDR组血清IL-6水平分别为（3.72±1.50）、（4.56±2.33）、（4.52±1.97）ng/L,对照组为（3.50±2.24）ng/L,实验组与对照组比较差异无统计学意义（F=1.430,P=0.240）;NDR组、NPDR组和PDR组房水IL-6水平分别为（128.42±32.22）、（205.15±25.15）、（1 618.00±42.66）ng/L,对照组为（115.80±29.97）ng/L,实验组与对照组比较差异有统计学意义（F=9 851.950,P=0.000）;NDR组房水IL-6水平与对照组比较差异无统计学意义（P〉0.05）,NPDR组与PDR组房水IL-6水平均高于对照组（P〈0.01）,且随眼部病情加重,有增高趋势（P〈0.01）。结论房水IL-6水平的表达与DR程度相关。     

糖尿病微血管病变与血清同型半胱氨酸、氧化应激反应的相关性

目的 探讨糖尿病微血管病变患者血清同型半胱氨酸与氧化应激反应的相关性.方法 测定80名健康人(对照组),100例无微血管病变2型糖尿病患者(DM组)、100例合并糖尿病肾病2型糖尿病患者(DN组)及100例合并DR2型糖尿病患者(DR组)血清同型半胱氨酸(Hcy)、丙二醛(MDA)、超氧化物歧化酶(SOD)及还原型谷胱甘肽(GSH)浓度.结果 DM组、DN组、DR组Hcy浓度分别为(98.86±21.46)、(198.95±19.35)、(138.65±15.25)ng/L,MDA浓度分别为(17.49±1.64)、(28.89±2.14)、(22.47±1.86)nmol/L,均明显高于对照组(62.48±15.36)ng/L,(11.86±0.48)nmol/L)(F值分别为7.95、6.89,P均＜0.01);DN组及DR组均较DM组明显升高(P均＜0.01),DM组、DN组、DR组血清SOD浓度分别为(107.80±15.62)、(79.86±14.63)、(89.34±12.75)mg/L,GSH浓度分别为(179.26±25.81)、(143.36±21.75)、(156.96±19.35)mg/L,均低于正常对照组(128.32±19.21)mg/L,(237.38±27.31)mg/L(F值分别为7.89、8.76,P均＜0.01);DN组及DR组均较DM组低(P均＜0.01);同时DN组又低于DR组(P＜0.01);血清Hcy与MDA浓度呈正相关(r=0.79,P＜0.05),与SOD、GSH浓度均呈负相关(r值分别为-0.71、-0.78,P均＜0.05).结论 糖尿病微血管病变患者血清同型半胱氨酸浓度升高,氧化应激反应增强,氧化应激与血清同型半胱氨酸浓度升高有关,血清同型半胱氨酸浓度升高,氧化应激促进糖尿病微血管病变发生发展.     

细胞因子VEGF、IGF-1与2型糖尿病视网膜病变的相关性研究

目的探讨血浆VEGF、IGF-1水平与2型糖尿病视网膜病变的相关性。方法选择2型糖尿病患者60例,分为2型糖尿病不伴有视网膜病变组(NDR)20例;2型糖尿病伴视网膜病变(非增值期)组(BDR)20例;糖尿病伴视网膜病变(增值期)组(PDR)20例;健康对照组20例,经口服葡萄糖耐量试验证实排除糖尿病。分别检测空腹血糖、糖化血红蛋白、甘油三脂、总胆固醇、低密度脂蛋白胆固醇及细胞因子VEGF、IGF-1等各项指标。结果①VEGF水平:2型糖尿病各组均高于正常对照组;BDR组明显高于NDR组,但PDR组VEGF含量下降;②IGF-1水平:呈正常对照组相似文献   

糖化血红蛋白、糖化血清蛋白、空腹血糖与糖尿病视网膜病变的关系

目的探讨糖尿病患者糖化血红蛋白（HbA1 c）、糖化血清蛋白（GSP）、空腹血糖（FBG）与视网膜病变的关系。方法对220例2型糖尿病患者进行HbA1 c、GSP及FBG检测并做眼底检查。其中糖尿病正常眼底（NDR）组112例,糖尿病视网膜病变（DR）组108例。结果 DR组HbA1 c及GSP值明显高于NDR组（P〈0.01）,而FBG水平DR组与NDR组差异无统计学意义（P〉0.05）。结论 HbA1 c及GSP可作为糖尿病患者发现DR的指标,两者结合考虑更有助于DR的发现。     

糖尿病大鼠视网膜血管内皮生长因子和一氧化氮的变化

背景 糖尿病患者外周血中的血管内皮生长因子 (vascular endothelial growth factor,VEGF)和一氧化氮存在正相关,但糖尿病时视网膜组织的 VEGF和一氧化氮是否发生变化尚不清楚.目的 探讨糖尿病大鼠视网膜 VEGF和一氧化氮的变化及其在糖尿病视网膜病变( diabetic retinopathy,DR)发生发展过程中的作用.设计 随机对照实验研究.地点和材料 选择健康成年 SD大鼠(上海 SIPPR-BK实验室提供,合格证 152),在福建医科大学动物实验室喂养.实验在福建医科大学解剖学与组织胚胎学实验室完成.干预 随机分成正常对照组、糖尿病 1月、糖尿病 3月和糖尿病 5月组,每组 20只.除正常组外所有大鼠均一次性腹腔注射链脲佐菌素( streptozotin,STZ)诱发糖尿病模型.应用免疫组织化学方法观察各组视网膜组织 VEGF的表达情况,并检测各组视网膜组织中一氧化氮合酶( nitric oxide synthase,NOS)活性和一氧化氮含量变化.主要观察指标 ①各组视网膜组织 VEGF的表达情况.②各组大鼠视网膜组织中 NOS活性和一氧化氮含量变化.结果 正常大鼠视网膜 VEGF免疫阳性反应仅见于内核层细胞, NOS和一氧化氮含量分别为( 36.17± 1.83) μ kat/g 和 (36.77± 2.33) μ mol/g; 3个月时,糖尿病大鼠视网膜 VEGF表达增强, NOS和一氧化氮含量分别为 (115.19± 31.5) μ kat/g 和 (68.34± 5.46) μ mol/g,与正常组比较,差异有显著性意义( t=7.43,8.15;P《 均 0.01); 5个月时,糖尿病大鼠视网膜表达进一步增强, NOS和一氧化氮含量分别为 (72.35± 16.34) μ kat/g 和 (50.62± 3.01) μ mol/g,与糖尿病 3月组比较,差异有显著性意义( t=4.76, 4.19;P均 《 0.05).结论 糖尿病大鼠视网膜 VEGF的过度表达和 NOS活性、一氧化氮含量的变化在 DR的发生发展过程中起重要作用.     

人类巨细胞病毒与2型糖尿病视网膜病变关系的研究

目的:探讨人类巨细胞病毒(human cytomegalovirus,HCMV)感染与2型糖尿病视网膜病变(diabetic retinopathy,DR)的关系。方法:采用聚合酶链反应(chain reaction technique,PCR)技术分别检测2型糖尿病(T2DM)患者86例(单纯T2DM组40例、DR组46例)以及对照组30例外周血中的HCMV-DNA,放射免疫法测定血浆内皮素(endothelin,ET)。结果:DR组HCMV-DNA检测阳性率(65%)明显高于单纯T2DM组(35%)和对照组(13%,P<0.01),DR组ET含量与单纯T2DM组和对照组比较差异也有显著性。结论:HCMV感染与DR的发生发展有关,HCMV感染可能加重DR。     

2型糖尿病患者糖尿病视网膜病变检出率及其相关危险因素分析

目的：探讨2型糖尿病患者糖尿病视网膜病变（DR）的发生率及相关危险因素。方法：收集448例2型糖尿病患者的眼底检查情况及其他临床资料,根据眼底检查结果将患者分为糖尿病无视网膜病变（NDR）及糖尿病视网膜病变（DR）组,比较可能诱发DR的危险因素。结果：112例患者合并DR占总数的25．0％。年龄、糖尿病病程、收缩压、尿素氮、肌酐、尿微量白蛋白以及高血压、脑梗死合并率两组之间的差异有统计学意义。Logistic回归糖尿病病程、尿微量白蛋白是DR发生的独立危险因素。结论：我院住院2型糖尿病患者中25．0％合并DR,糖尿病病程、尿微量白蛋白是DR独立的危险因素。