粒细胞集落刺激因子动员2型糖尿病患者外周血CD34+细胞的变化

背景：与正常人比较,2型糖尿病患者外周血CD34+细胞含量已有明显下降。 目的：分析2型糖尿病患者外周血动员后CD34+细胞比例的变化。 方法：将234例2型糖尿病患者按病程分为5组(<1年,1~5年,>5~<10年,10~15年和≥15年),予以粒细胞集落刺激因子动员。动员5 d后,使用流式细胞仪检测外周血CD34⁺细胞的含量,并利用Person简单相关及多元回归方法分析其与病程、血脂、尿酸的关系。 结果与结论：糖尿病者外周血动员后CD34⁺细胞水平与三酰甘油相关(r=-0.202,P=0.002),与载脂蛋白B相关(r=-0.276,P=0.000),与尿酸相关(r=-0.297,P=0.000)。经统计分析发现,糖尿病者外周血动员后CD34⁺细胞数随糖尿病病程进展而逐渐下降。     

慢性阻塞性肺疾病频繁急性加重患者的临床特征分析

目的 观察慢性阻塞性肺疾病（COPD）频繁急性加重患者与非频繁急性加重患者临床特征的差异。方法 回顾性纳入2016年1月~2018年12月合肥市第一人民医院呼吸内科住院的274例COPD急性加重患者,根据患者上一年急性加重次数分为频繁组（住院次数≥2次/年）143例和非频繁组（住院次数＜2次/年）131例,比较两组性别、年龄、病程、吸烟量、mMRC评分、共患疾病（高血压、冠心病、糖尿病）、白细胞计数、中性粒细胞百分比、淋巴细胞百分比、嗜酸粒细胞百分比、降钙素原（PCT）、血气指标（pH、PaO2、PaCO2）及血氧饱和度（SpO2）,多因素Logistic回归分析COPD频繁急性加重的危险因素。结果 两组性别、年龄、白细胞计数、淋巴细胞百分比、嗜酸粒细胞百分比、PCT、pH、PaCO2、共患疾病（高血压、冠心病、糖尿病）比较,差异无统计学意义（P＞0.05）;频繁组病程、吸烟量、mMRC评分、中性粒细胞百分比、SpO2、PaO2均高于非频繁组,差异均有统计学意义（P＜0.05）;多因素Logistic回归分析显示,病程、吸烟量、mMRC评分、中性粒细胞百分比是COPD频繁急性加重的独立危险因素。结论 COPD频繁急性加重多病程较长,患者多长期吸烟,临床中应重视病程长、吸烟量大、mMRC评分高、中性粒细胞百分比高的COPD患者,加强对其的监护管理,防止发生COPD频繁急性加重。     

亲属活体供肾移植中供者肾功能的评估

背景：肾移植前正确有效地评价供者双侧肾脏功能,对于亲属活体肾移植供者及受者的安全十分重要。 目的：探讨亲属活体供肾移植中供者肾功能评估的指标。 方法：173例亲属活体肾移植供者年龄分成老年组(≥55岁)和中青年组(< 55岁),两组供者移植前血清肌酐、总肾小球滤过率、欲保留肾总肾小球滤过率、内生肌酐清除率、血尿素氮反应肾功能指标差异无显著性意义,分析比较移植前后肾功能各指标的变化。 结果与结论：与移植前比较,移植后10 d供者血清肌酐、血尿素氮增高,内生肌酐清除率降低(P < 0.01);移植后1个月留存肾脏总肾小球滤过率增加(P=0.000 0),但与移植前供者双肾比较下降。提示总肾小球滤过率可作为活体亲属肾移植供者肾功能评估的主要指标,但是仍应结合血清肌酐、内生肌酐清除率等指标综合分析。     

糖尿病患者冠状动脉药物洗脱支架内晚期丢失及非靶病变进展的影响因素

背景：已有研究认为,冠心病合并2型糖尿病患者冠状动脉粥样硬化斑块进展和冠状动脉支架内再狭窄发生风险增加。  目的：探讨2型糖尿病患者支架内再狭窄和非靶病变进展情况及其影响因素。 方法：纳入399例冠心病接受冠状动脉支架置入患者,根据是否合并糖尿病将患者分为糖尿病组(n=179)和非糖尿病组(n=220),收集一般资料、冠状动脉造影及支架置入相关参数;将糖尿病组根据是否发生支架内再狭窄分为再狭窄组(n=66)和无再狭窄组(n=113),根据有无非靶病变快速进展分为非靶病变进展组(n=48)和非靶病变无进展组(n=131),检测支架置入3,120,210,360 d的血低密度脂蛋白胆固醇、糖化血红蛋白、血浆纤维蛋白原和超敏C-反应蛋白水平。 结果与结论：与非糖尿病组比较,糖尿病组冠状动脉支架长度更长(P=0.018),支架直径更小(P=0.002),支架置入后即刻和造影随访的最小管腔直径更小(P=0.001,P=0),支架置入后即刻和造影随访的冠状动脉狭窄程度更严重(P=0.038,P=0.004),造影随访晚期管腔丢失和再狭窄发生比例更多(P=0,P=0.097)。在糖尿病患者的亚组分析中,再狭窄者的血浆纤维蛋白原、超敏C-反应蛋白和糖化血红蛋白水平较无再狭窄者更高;非靶病变进展者的血浆纤维蛋白原、超敏C-反应蛋白和糖化血红蛋白水平较非靶病变未进展者更高。表明糖尿病患者发生支架内再狭窄和非靶病变进展的比例较高,同时血浆纤维蛋白原、超敏C-反应蛋白和糖化血红蛋白等生化指标可辅助预测再狭窄和非靶病变进展的发生。中国组织工程研究杂志出版内容重点：生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程全文链接：     

2型糖尿病患者血清von Willebrand因子变化与微血管并发症的关系

目的 :探讨 2型糖尿病患者的微血管内皮细胞功能损伤与微血管并发症之间的关系。方法 :从 3 3 4名 2型糖尿病患者中选择合并微血管并发症但未合并大血管并发症者 3 2人作为微血管并发症组 ,并选择年龄与之匹配的无大血管及微血管并发症 55人作为无并发症组 ,观察二组患者血清vonWillebrand因子 (vWF)水平 ,并与年龄相接近的正常对照组 ( 4 0例 )比较。结果 :血清vWF水平正常对照组 0 .92U± 0 .44U/ml;无并发症组 1.15U± 0 .42U /ml ;微血管并发症组 1.3 7U± 0 .44U/ml;三组之间均有显著性差异 (P <0 .0 5～ 0 .0 1)。多因素Logistic回归分析表明vWF、空腹血糖分别与糖尿病人是否合并微血管病变显著相关 (ExpB分别为 3 .0 2 3 ,1.3 3 7,P <0 .0 5～ 0 .0 1) ,以vWF为应变量的多元逐步回归分析表明年龄、糖尿病病程、甘油三酯分别与vWF呈显著独立正相关 (B =0 .53 ,0 .3 5,0 .2 9,P <0 .0 5～ 0 .0 1)。结论 :在糖尿病患者中存在不同程度的微血管内皮细胞的损伤 ,此变化随着微血管并发症的进展呈进行性加重。以vWF升高所反映的微血管内皮细胞损伤是一项值得临床推广的研究方法     

2型糖尿病患者外周血白细胞、中性粒细胞/淋巴细胞比值与泌汗功能相关性研究

目的 研究2型糖尿病患者外周血白细胞(WBC)计数、中性粒细胞/淋巴细胞比值(NLR)与泌汗功能的相关性.方法 选取2020年4月至2021年2月在本院内分泌科住院的2型糖尿病患者267例,所有患者均完善Sudoscan仪检测.根据双手及双足电导率(HESC、FESC)结果的不同将所有患者分为2组:泌汗功能正常组(NSF组,n=141)和泌汗功能异常组(ASF组,n=126).同时收集相关临床和实验室检测指标,分析2组之间各指标差异,对影响泌汗功能的危险因素进行多元线性回归分析,并绘制受试者工作特征(ROC)曲线评估NLR的预测效能.结果 NSF组和ASF组在年龄、病程、舒张压、中性粒细胞/淋巴细胞差异均有统计学意义(P<0.05),ASF组中性粒细胞/淋巴细胞高于NSF组.两组在性别、收缩压、BMI、空腹血糖、餐后2h血糖、糖化血红蛋白、白细胞、三酰甘油、胆固醇、高密度脂蛋白、低密度脂蛋白、肌酐、尿素氮、尿酸、HOMA-IR、HOMA-islet间差异均无统计学意义(P>0.05);多元线性回归模型表明NSF组与ASF组病程、中性粒细胞/淋巴细胞差异存在统计学意义(P<0.05),年龄、舒张压两组间差异无统计学意义(P>0.05);NLR的分界点为1.86,灵敏度为77.4％,特异性为70.0％,曲线下面积为0.790,(95％CI 0.737～0.844,P<0.001).结论 病程及NLR与2型糖尿病患者泌汗功能障碍呈正相关,且NLR诊断泌汗功能损伤的灵敏度较高、特异性尚可.     

尿Cys C和β2-MG检测在2型糖尿病肾脏功能早期损伤评估中的价值

目的 探讨2型糖尿病患者肾功能早期损伤的实验室指标。方法 对72例2型糖尿病患者，测定血糖（Glu）、血肌酐（Cr）、总胆固醇（Tc）、高密度脂蛋白胆固醇（HDL-C）和甘油三酯（TG），计算内生肌酐清除率，分为滤过正常组（Ccr≥70ml／min）和滤过异常组（Ccr〈70ml／min）。测定尿β2-微球蛋白（β2-MG）、尿微量白蛋白（MA）、尿转铁蛋白（TRF）、尿Igc（IgU）、尿α1微球蛋白（α1-MG）和血清／尿胱抑素C（Cysc）。结果 2组糖尿病患者，滤过异常组尿MA和Cystatin C浓度显著高于滤过正常组。滤过正常组除IgU外与对照组相比均有显著性差异，β2-MG和α1MG与对照组相比有极显著性差异；滤过异常组与对照组及滤过正常组相比均有极显著性差异；滤过正常组尿β2-MG高于正常的9人，占20．9％，尿Cys C高于正常的患者共37人，占86％。结论 2型糖尿病患者肾功能早期损伤是肾小球重吸收功能降低先于肾小球滤过率降低，尿液中Cys C和β2-MG的联合检测能更准确的反映肾小管的重吸收功能。     

国产雷帕霉素药物涂层支架置入ST抬高型心肌梗死患者的近期预后

背景：冠状动脉药物涂层支架置入治疗是急性ST段抬高型心肌梗死的最佳治疗方法,但由于老年患者合并危险因素较多,死亡率随着年龄的增加而增高。 目的：观察不同年龄ST段抬高型心肌梗死患者置入国产雷帕霉素药物涂层支架的临床特征及住院期间预后特点。 方法：回顾性分析307例因初发ST段抬高型心肌梗死接受急诊冠状动脉国产雷帕霉素药物涂层支架置入治疗患者的临床资料,根据年龄分为3组,非老年组(< 65岁,n=175)、普通老年组(65-74岁,n=83)、高龄组(≥75岁,n=49),比较3组住院期间的临床特征、病死率及联合心血管事件发生情况。 结果与结论：与非老年组相比,普通老年组、高龄组女性比例较高(P < 0.05),吸烟者较少(P < 0.05),既往高血压病史比例增加(P < 0.05),血红蛋白、总胆固醇、三酰甘油、低密度脂蛋白胆固醇水平降低(P < 0.05)。随着年龄的增长,血清肌酐水平逐渐升高,恶性心律失常发生率与住院期间心源性死亡发生率逐渐增加。与非老年组、普通老年组比较,高龄组心功能Killip分级≥Ⅱ级比例、3支或左主干病变比例较高(P < 0.05),成功再灌注率下降(P < 0.05),急性充血性心力衰竭、联合心血管事件的发生率较高(P < 0.05)。多因素Logistic回归分析显示,年龄是患者住院期间病死率的独立危险因素(P < 0.01)。中国组织工程研究杂志出版内容重点：生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程全文链接：     

三种不同肾功能检测指标与DR关系的探讨

目的：探讨尿白蛋白、尿白蛋白/尿肌酐、肾小球滤过率三种不同肾功能指标与2型糖尿病视网膜病变（DR）之间的关系。方法：124例DM2分为不伴视网膜病变（A组）51例,视网膜病变单纯型（B组）50例和视网膜病变增殖型（C组）23例,各组均行尿素氮、肌酐及24 h尿白蛋白（UAlb）、尿白蛋白/尿肌酐（ACR）、肾动态显像（GFR-ECT）及其相关指标检测并对结果进行分析。结果：C组与A、B组比较,UAlb、ACR明显增加,GFR明显下降（P〈0.05）。相关分析显示,UAlb、ACR及肾小球滤过率（GFR）是糖尿病视网膜病变发病的独立危险因素。结论：DR程度是随尿白蛋白、尿白蛋白/尿肌酐增加和肾小球滤过率减低而加重的;同时研究显示,GFR对早期诊断糖尿病肾病（DN）有较高的临床价值。     

国产可降解雷帕霉素药物洗脱支架置入的疗效评价：与金属裸支架比较

背景：药物洗脱支架明显降低了再狭窄率,但永久聚合物涂层在抑制血管平滑肌细胞增生的同时,阻碍血管完全内皮化进程,促使再狭窄发生,且永久聚合物涂层可引发晚期血栓等并发症。 目的：观察冠心病合并糖尿患者置入国产可降解药物洗脱支架后的远期疗效和安全性,并与置入金属裸支架患者进行比较。 方法：实验组选择冠心病合并2型糖尿病患者136例,首次经皮冠状动脉成形治疗,置入国产可降解药物洗脱支架(EXCELTM),并以同期行金属裸支架置入治疗的冠心病合并2型糖尿病患者87例为对照组,随访12个月以上,记录主要不良心血管事件发生情况,并复查冠状动脉造影。 结果与结论：实验组129例,对照组83例完成(13.5±3.5)个月随访,实验组及对照组分别有8例及12例患者发生不良心血管事件,两组间差异有显著性意义(P=0.045),对照组靶血管血运重建及再发心绞痛多于实验组(P < 0.05)。两组患者随访冠状动脉造影的定量分析提示实验组最终丢失指数小于对照组(P < 0.05),再狭窄率低于对照组(P < 0.05)。说明对于冠心病合并糖尿病患者,应用国产可降解药物洗脱支架可降低不良心血管事件发生率,减少再狭窄,改善患者远期预后,安全性能良好。     

Endothelial dysfunction and microvascular complications in type 1 diabetes mellitus

We examined whether alterations in vascular endothelial function and early structural changes in atherosclerosis are associated with microvascular complications in patients with type 1 diabetes mellitus (DM). Flow-mediated dilation (FMD) of the brachial artery and carotid intima-media thickness (IMT) measurement were performed in 70 young adults (aged 19 to 35 yr), 48 with type 1 DM, and 22 normal controls. Patients with diabetes had a lower peak FMD response (7.8+/-3.9 vs. 11.1+/-1.9%, p<0.001) and increased IMT (0.51+/-0.10 vs. 0.42+/-0.07 mm, p<0.001) compared with controls. Twenty (41.7%) of the patients had microvascular complications including neuropathy, nephropathy, or retinopathy. In these complicated diabetic patients, we found a lower FMD response (6.1+/-2.5 vs. 9.9+/-3.5%, p=0.001) compared with diabetics without microvascular complications. The presence of microvascular complications was also associated with older age and longer duration of the disease. However, no differences were observed in IMT, body size, blood pressure, HbA1c, C-reactive protein, low-density lipoprotein or high-density lipoprotein cholesterol levels between complicated and non-complicated patients. Endothelial dysfunction and early structural atherosclerotic changes are common manifestations in type 1 DM, and endothelial dysfunction is thought to be an early event in the atherosclerotic process and important in the pathogenesis of microvascular complications.     

糖尿病视网膜病变与血管性血友病因子和纤维蛋白原的关系

目的 :探讨糖尿病视网膜病变 (DR)与纤维蛋白原 (FIB)及vonWillebrand因子 (vWF)水平的关系及其临床意义。方法 :2型糖尿病人 10 4例根据病史及是否合并糖尿病微血管病变进行如下分组 :无并发症组 5 3例 (男 2 6例 ,女 2 7例 ) ,合并糖尿病视网膜病变组 5 1例(男 2 5例 ,女 2 7例 ) ,正常对照组 5 6例 (男 2 8例 ,女 2 8例 )。三组分别采用硫酸胺比浊法及双抗酶联免疫吸附法 (EUA)分别进行FIB、vWF的测定 ,并测定血压、糖化血红蛋白等指标。结果 :糖尿病患者血浆vWF、FIB水平较正常对照组明显升高 (P <0 .0 1) ,合并糖尿病视网膜病变组较无并发症组显著升高 (P <0 .0 1)。结论 :血浆FIB及vWF水平的升高与糖尿病视网膜病变的发生发展有关。     

糖尿病微血管病患者骨密度及骨钙素测定的意义

目的：探讨糖尿病微血管病变对骨密度及骨钙素水平的影响。方法：选择2型糖尿病患者60例，按其是否合并糖尿病微血管病(眼病、肾病、神经病变)分为两组，合并微血管病(1组)33例，不合并微血管病(2组)27例。用生化法测定两组的空腹血糖(FBG）、果糖胺(GSP)、血清总碱性磷酸酶(TALP)及血钙(Ca^2 )，RIA测定骨钙素(BGP)，DEXA法测定腰椎和髋部骨密度(BMC)；按其身高、体重计算体重指数(BMI)。结果：两组BBMI、GSP、TALP及Ca^2 均未见明显差异；1组血清BGP水平明显低于2组，有显著性差异；1组第2—4腰椎（L2-4)、股骨颈、Ward’s三角区及股骨大转子的BMD均低于2组，差异有显著性。结论：骨密度及骨钙素与糖尿病微血管病变关系密切。认为糖尿病微血管病可能降低骨形成，加重骨质疏松。     

Autologous stem cell transplantation for early type 1 diabetes mellitus

Type 1 diabetes mellitus (T1DM) is the result of the autoimmune response against pancreatic insulin producing β cells. This autoimmune response begins months or even years before the first presentation of signs and symptoms of hyperglycemia and at the time of clinical diagnosis near 30% of β-cell mass still remains.

In daily clinical practice, the main therapeutic option for T1DM is multiple subcutaneous insulin injections that are shown to promote tight glucose control and reduce much of diabetic chronic complications, especially microvascular complications.

Another important aspect related to long-term complications of diabetes is that patients with initially larger β-cell mass suffer less microvascular complications and less hypoglycemic events than those patients with small β-cell mass. In face of this, β-cell preservation is another important target in the management of type 1 diabetes and its related complications.

For many years, various immunomodulatory regimens were tested aiming at blocking autoimmunity against β-cell mass and at promoting β-cell preservation, mainly in secondary prevention trials.

In this review, we summarize some of the most important studies involving β-cell preservation by immunomodulation and discuss our preliminary data on autologous nonmyeloablative hematopoietic stem cell transplantation in newly-diagnosed T1DM.     

2型糖尿病血管病变时血浆ANP、BNP及CNP的变化及临床意义

目的：探讨血浆心钠素(ANP)、脑利钠肽(BNP)、C型利钠肽(CNP)在2型糖尿病血管病变时的变化及其临床意义。方法:应用酶联免疫吸附法(ELISA)测定正常对照组(9例)、2型糖尿病无血管病变组(34例)及2型糖尿病血管病变组(23例)血浆proANP、BNP fragment及NT-proCNP浓度，分析各组间血浆利钠肽水平的变化及相关因素。结果:2型糖尿病血管病变组血浆ANP、BNP明显高于另外2组（P<0.01），而血浆CNP明显降低（P<0.01），2型糖尿病血管病变组各亚组(微血管病变组、大血管病变组及微血管合并大血管病变组)间血浆利钠肽水平无明显差异（P>0.05）。2型糖尿病血管病变组血浆ANP与BNP间存在显著正相关（r=0.309, P<0.05），ANP与CNP（r=-0.374, P<0.05）以及BNP与CNP（r=-0.653, P<0.01）间存在显著负相关。结论:血浆ANP、BNP及CNP的联合检测可以作为简便、价廉、可靠的糖尿病血管病变的筛选指标。     

miRNAs as cornerstones in diabetic microvascular complications

Diabetes mellitus is usually accompanied by nephropathy, retinopathy, and neuropathy as microvascular complications. MicroRNAs (miRNAs) can affect the kidney, retina, and peripheral neurons through their implication in pathways involved in angiogenesis, inflammation, apoptosis, as well as fibrosis within these tissues and hence, play a crucial role in the pathogenesis of microvascular complications. In this review, the updated knowledge of the role of miRNAs in the pathogenesis of diabetic microvascular complications was summarized. PubMed Central was searched extensively to retrieve data from a wide range of reputable biomedical reports/articles published after the year 2000 to systematically collect and present a review of the key molecular pathways mediating the hyperglycemia-induced adverse effects on vascular tissues, particularly in persons with T2DM. In the present review, miR-126, miR-29b, and miR-125a are implicated in diabetes-induced microvascular complications, while miR-146a is found to be connected to all these complications. Also, vascular endothelial growth factors are noted to be the most impacted targets by miRNAs in all diabetic microvascular problems.     

Direct medical costs for patients with type 2 diabetes and related complications: a prospective cohort study based on the korean national diabetes program

We analyzed the direct medical costs for Korean patients with type 2 diabetes according to the type of complications and the number of microvascular complications. We analyzed costs for type 2 diabetes and associated complications in 3,125 patients. These data were obtained from the Korean National Diabetes Program (KNDP), a large, ongoing, prospective cohort study that began in 2005. The cost data were prospectively collected, using an electronic database, for the KNDP cohort at six hospitals. The costs were analyzed according to complications for 1 yr from enrollment in the study. Among 3,125 patients, 918 patients had no vascular complications; 1,883 had microvascular complications only; 51 had macrovascular complications only; and 273 had both complications. The annual direct medical costs for a patient with only macrovascular, only microvascular, or both macrovascular and microvascular complications were 2.7, 1.5, and 2.0 times higher than the medical costs of patients without complications. Annual direct medical costs per patient increased with the number of microvascular complications in patients without macrovascular complications. The economic costs for type 2 diabetes are attributable largely to the management of microvascular and macrovascular complications. Proper management of diabetes and prevention of related complications are important for reducing medical costs.     

The role of vitamin E and oxidative stress in diabetes complications

Diabetes is a disease characterized by poor glycemic control for which risk of the type 2 form increases with age. A rise in blood glucose concentration causes increased oxidative stress which contributes to the development and progression of diabetes-associated complications. Studies have shown that primary antioxidants or genetic manipulation of antioxidant defenses can at least partially ameliorate this oxidative stress and consequentially, reduce severity of diabetic complications in animal models. Data from humans is less clear and will be summarized in this review. We highlight results from studies performed to investigate the role of vitamin E in preventing diabetes-induced oxidative damage in cell culture, animal models, and human participants, and summarize evidence testing whether this nutrient has an effect on outcomes related to the diabetic complications of nephropathy, retinopathy, and neuropathy. The most compelling evidence for an effect of vitamin E in diabetes is on protection against lipid peroxidation, whereas effects on protein and DNA oxidation are less pronounced. More studies are required to make definitive conclusions about the effect of vitamin E treatment on diabetes complications in human subjects.     

Targeting receptors of advanced glycation end products (RAGE): Preventing diabetes induced cancer and diabetic complications

Cancer and diabetes are the two major disorders that affect a large proportion of the world’s population. Results from multiple epidemiological studies have concluded that diabetes and cancer are linked, and diabetic patients live at much higher risks of developing cancer and diabetic complications at the later phase of disease. Inflammation is the central pathway that mediates both diabetic complications as well as cancer. Receptor of advanced glycation end products (RAGE) is a non-specific multi-ligand pattern recognition receptor that induces the inflammatory responses by binding with multiple ligands. RAGE and its ligands are upregulated in diabetes, inflammation and cancer. Advanced glycation end products (AGEs), high mobility group box protein-1 (HMGB1) and S100 proteins are the major RAGE ligands that contribute to these consequences and an increased release of RAGE ligands during diabetic conditions can be a possible mechanism leading to diabetic complications and cancer. Moreover, further release of RAGE ligands from cancer cells can be a possible mechanism behind the worsening of diabetic complications in diabetic cancer patients. Inhibition of RAGE signaling can prevent diabetic complications and cancer in diabetic patients and can be helpful in the management of worsening diabetic complications and cancer in diabetic cancer patients. Curcumin, Quercetin and Withaferin A are known to inhibit multiple molecular pathways that are involved in RAGE signaling. The combined effects of these molecules can be explored to achieve the complete inhibition of RAGE signaling in diabetic patients.     

Concentrations of 7S collagen and laminin P1 in sera of patients with diabetes mellitus

Summary Specific radioimmunoassays were used to quantify two basement membrane components, 7S collagen and laminin P1, in sera of 70 patients suffering from diabetes mellitus types I and II with and without clinical signs of chronic diabetic complications. Serum levels of both antigens were increased in diabetics compared to controls (p<0.001). Concentrations of 7S collagen were significantly different in diabetics with signs of microvascular damage compared to those without small vessel disease (p<0.05). The difference between laminin P1 concentrations in the two groups of diabetics was not significant (p<0.2). The augmented levels of circulating 7S collagen and laminin P1 may reflect alterations of basement membrane metabolism. Thus, the measurement of concentrations of these basement membrane components in serum may be a useful tool for monitoring the development of chronic diabetic complications.Supported by grants of the Deutsche Forschungsgemeinschaft (Ho 980/1-1, Ra 255/6-1, Vo 339/1-2)