肿瘤细胞DNA倍体和P53蛋白表达分析

探索Ｐ５３蛋白表达与ＤＮＡ倍体以及与其他临床指标的关系。方法我们用流式细胞仪双参数检测了７１例临床肿瘤手术标本的ＤＮＡ含量的Ｐ５３蛋白表达。其中良性肿瘤８例，卵巢癌４２例，肺癌１２例，乳腺癌９例。同时用免疫组化方法检测上述本３２例的石蜡切片，作Ｐ５３蛋白表达检测。     

维吾尔族妇女子宫颈癌中 p53 基因的表达和 HPV DNA 检测研究

应用Ｅ６特异性引物ＰＣＲ检测新疆维吾尔族妇女子宫颈癌活检组织标本中的ＨＰＶ６，１６和１８ＤＮＡ。并应用免疫组化研究ｐ５３基因的表达。结果显示，６５例标本中７例不适应用于ＰＣＲ，其余５８例中ＨＰＶ１６ＤＮＡ阳性４５例（７７．６％），而ＨＰＶ６ＤＮＡ和１８ＤＮＡ为阴性。免疫组化显示，６５例中ｐ５３表达阳性４９例（７５．４％）。在４５例ＨＰＶＤＮＡ阳性标本中，ｐ５３表达阳性３４例（７５．６％）；１３例ＨＰＶＤＮＡ阴性标本中，ｐ５３表达阳性１１例（８４．６％）。提示，ＨＰＶ阳性或阴性与ｐ５３表达之间无显著性差异（Ｐ＞０．０５）。     

卵巢恶性肿瘤P53基因表达产物及相关生物学行为的初步分析

目的：探讨Ｐ５３基因突变表达产物与卵巢肿瘤临床生物学行为之间的关系。方法：采用单克隆抗体免疫组化染色方法对５０例卵巢恶性肿瘤，１０例良性肿瘤的石蜡切片标本进行Ｐ５３基因突变蛋白表达检测。结果：卵巢恶性肿瘤Ｐ５３阳性表达率２４％（１２／５０），卵巢良性肿瘤均为阴性卵巢上皮癌阳性结果为２９％（８／２８），生殖细胞瘤１０％（１／１０），间质瘤２５％（２／８），转移瘤２５％（１／４）；不同临床分期阳性表达     

流式细胞光度术分析胃癌石蜡标本DNA含量及P53蛋白含量的测定

用免疫组织化学的方法检测了４９例胃癌组织和１０例胃良性病变组织Ｐ５３蛋白的含量，用流式细胞光度术检测了ＤＮＡ倍体和１０例免疫组化Ｐ５３蛋白表达阳性标本的Ｐ５３蛋白含量。结果提示，ＤＮＡ倍体和Ｐ５３蛋白表达与胃癌生物学行为有重要关系，ＦＣＭ定量基因表达产物及ＤＮＡ含量双参数分析预后，对临床有重要指导意义。     

流式细胞光度术分析胃癌石蜡标本DNA含量及P53蛋白含量的…

用免疫组织化学的方法检测了４９例胃癌组织和１０例胃良性病变组织Ｐ５３蛋白的含量，用流式细胞光度术检测了ＤＮＡ倍体和１０例免疫组化Ｐ５３蛋白表达阳性标本的Ｐ５３蛋白含量。结果提示，ＤＮＡ倍体和Ｐ５３蛋白表达与胃癌生物学行为有重要关系，ＦＣＭ定量基因表达产物及ＤＮＡ含量双参数分析预后，对临床有重要指导意义。     

血管免疫母细胞性淋巴结病P53蛋白表达及DNA含量分析

目的研究Ｐ５３蛋白在血管免疫母细胞性淋巴结病（ＡＩＬ）中的表达及ＤＮＡ含量,探讨ＡＩＬ的发生发展规律、病变性质。②方法应用免疫组化技术检测２０例ＡＩＬ病人的免疫表型及Ｐ５３蛋白表达,并用图像分析技术测定ＤＮＡ含量,同时选择非何杰金淋巴瘤（ＮＨＬ）２０例及淋巴结反应性增生病人１０例作为对比研究。③结果ＡＩＬ中Ｐ５３蛋白阳性表达率为３０％（６／２０）,与预后无关。ＤＮＡ含量改变对于病人预后有显著影响（ｔ＝２．８６４,Ｐ＜０．０５）。Ｐ５３蛋白表达、ＤＮＡ含量在ＡＩＬ与ＮＨＬ间差异均有显著性（χ２＝４．９１２,ｔ＝３．５５２,Ｐ＜０．０５）。ＡＩＬ中Ｐ５３蛋白阳性表达者ＤＮＡ含量显著高于阴性表达者（ｔ＝２．２２６,Ｐ＜０．０５）。④结论Ｐ５３蛋白表达及ＤＮＡ含量变化均与ＡＩＬ发生发展及恶性转化有关,ＡＩＬ可能为不同原因、不同性质的疾病共有的临床症候群。     

免疫组化法检测卵巢肿瘤P53蛋白表达

该组研究采用免疫组化法对正常卵巢组织３０例，良性上皮肿瘤３０例，交界性肿瘤１１例，性索间质瘤２１例，上皮性癌４０例，恶性生殖细胞瘤２１例进行了抑癌基因Ｐ５３蛋白表达的检测。Ｐ５３蛋白表达阳性例数分别为０，１（３．３％），２（１８．２％）。５（２３．８％）。２２（５５％），２３（６１．９％）。结果显示：卵巢良性上皮肿瘤、交界性肿瘤和性索间质瘤。上皮性癌和恶性生殖细胞性肿瘤三组间Ｐ５３蛋白阳性表达差异有显著性。（Ｐ＜０．０５）     

食管癌组织中 HPV 原位杂交及 E_6、P~(21ras)和 P~(53)免疫组化的研究

采用分子原位杂交和ＳＰ法免疫组化技术对柳州地区４０例食管鳞状细胞癌和２０例食管粘膜慢性炎的材料进行地高辛标记的ＨＰＶ６Ｂ／１１、１６、１８ＤＮＡ及ＨＰＶ１６、１８早期蛋白Ｅ６、Ｐ２１ｒａｓ和Ｐ５３癌基因产物检测，结果显示：食管癌中ＨＰＶ１６、１８ＤＮＡ及Ｅ６蛋白的阳性率分别为２５％（１０／４０）和６５％（２６／４０），与对照组对比差异均有高度显著性（Ｐ＜０．０１）。Ｅ６、和Ｐ５３蛋白呈双阳性者为５２．５０％（２１／４０），其中９０．４８％（１９／２１）阳性表达出现在同一区域同一癌细胞核内，似表达Ｅ６、可与Ｐ５３结合形成复合物，从而导致野生型Ｐ５３的降解或突变。本组鳞癌组织中Ｐ２１ｒａｓ与Ｐ５３、Ｐ５３与Ｅ６的阳性表达均具有显著相关性（Ｐ＜０．０５）。提示高危ＨＰＶ１６、１８感染与多癌基因协同在本地区食管癌病因学中起着十分重要的作用。     

Ⅰ期子宫内膜癌中p21ras的表达与DNA含量的研究

目的：探讨Ⅰ期子宫内膜癌ｐ２１ｒａｓ 表达及ＤＮＡ 含量。方法：ＡＢＣ免疫组化法及流式细胞术。结果：随组织学分级增高ｐ２１ｒａｓ表达率增高（Ｐ＜ ０．０５），ＤＮＡ 异倍体率亦增加（Ｐ＜ ０．０５）。异倍体子宫内膜癌中ｐ２１ｒａｓ阳性表达率（８２．３５％ ）较二倍体子宫内膜癌（３０．７７％ ）高（Ｐ＜ ０．０５）。结论：提示ｐ２１ｒａｓ作为子宫内膜癌预后参数的可能性，尤其是双参数同步分析将更好地评价子宫内膜癌预后     

膀胱移行细胞癌与突变型P^53蛋白表达的研究

对５１例膀胱移行细胞癌，９例正常膀胱粘膜及８例膀胱慢性炎组织，应用抗Ｐ５３蛋白单克隆抗体进行Ｓ－Ｐ免疫组化染色。结果表明：非癌组织均无Ｐ５３蛋白阳性表达，膀胱移行细胞癌阳性表达率为４９．０２％，且随分化程度的不同，阳性表达率有差异，０～Ⅰ级癌Ｐ５３蛋白阳性表达率为１９．０５％，Ⅱ～Ⅲ级癌Ｐ５３蛋白阳性表达率为７０．００％，两者之间差异有显著性（Ｐ＜０．０５）。此外，有淋巴结转移的移行细胞癌（Ｐ５３癌基因蛋白阳性表达率为６９．５％）与无淋巴结癌转移者（Ｐ５３癌基因蛋白阳性表达率为３２．１４％）两组之间差异有高度显著性（Ｐ＜０．０１）。上述结果提示检测Ｐ５３癌基因蛋白的表达情况可能成为膀胱移行细胞癌分级、估计淋巴结转移及预后的一项重要参考指标     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.