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1.
肿瘤细胞DNA倍体和P53蛋白表达分析 总被引:3,自引:0,他引:3
探索P53蛋白表达与DNA倍体以及与其他临床指标的关系。方法我们用流式细胞仪双参数检测了71例临床肿瘤手术标本的DNA含量的P53蛋白表达。其中良性肿瘤8例,卵巢癌42例,肺癌12例,乳腺癌9例。同时用免疫组化方法检测上述本32例的石蜡切片,作P53蛋白表达检测。 相似文献
2.
维吾尔族妇女子宫颈癌中 p53 基因的表达和 HPV DNA 检测研究 总被引:2,自引:0,他引:2
应用E6特异性引物PCR检测新疆维吾尔族妇女子宫颈癌活检组织标本中的HPV6,16和18DNA。并应用免疫组化研究p53基因的表达。结果显示,65例标本中7例不适应用于PCR,其余58例中HPV16DNA阳性45例(77.6%),而HPV6DNA和18DNA为阴性。免疫组化显示,65例中p53表达阳性49例(75.4%)。在45例HPVDNA阳性标本中,p53表达阳性34例(75.6%);13例HPVDNA阴性标本中,p53表达阳性11例(84.6%)。提示,HPV阳性或阴性与p53表达之间无显著性差异(P>0.05)。 相似文献
3.
目的:探讨P53基因突变表达产物与卵巢肿瘤临床生物学行为之间的关系。方法:采用单克隆抗体免疫组化染色方法对50例卵巢恶性肿瘤,10例良性肿瘤的石蜡切片标本进行P53基因突变蛋白表达检测。结果:卵巢恶性肿瘤P53阳性表达率24%(12/50),卵巢良性肿瘤均为阴性卵巢上皮癌阳性结果为29%(8/28),生殖细胞瘤10%(1/10),间质瘤25%(2/8),转移瘤25%(1/4);不同临床分期阳性表达 相似文献
4.
用免疫组织化学的方法检测了49例胃癌组织和10例胃良性病变组织P53蛋白的含量,用流式细胞光度术检测了DNA倍体和10例免疫组化P53蛋白表达阳性标本的P53蛋白含量。结果提示,DNA倍体和P53蛋白表达与胃癌生物学行为有重要关系,FCM定量基因表达产物及DNA含量双参数分析预后,对临床有重要指导意义。 相似文献
5.
流式细胞光度术分析胃癌石蜡标本DNA含量及P53蛋白含量的… 总被引:2,自引:0,他引:2
用免疫组织化学的方法检测了49例胃癌组织和10例胃良性病变组织P53蛋白的含量,用流式细胞光度术检测了DNA倍体和10例免疫组化P53蛋白表达阳性标本的P53蛋白含量。结果提示,DNA倍体和P53蛋白表达与胃癌生物学行为有重要关系,FCM定量基因表达产物及DNA含量双参数分析预后,对临床有重要指导意义。 相似文献
6.
目的研究P53蛋白在血管免疫母细胞性淋巴结病(AIL)中的表达及DNA含量,探讨AIL的发生发展规律、病变性质。②方法应用免疫组化技术检测20例AIL病人的免疫表型及P53蛋白表达,并用图像分析技术测定DNA含量,同时选择非何杰金淋巴瘤(NHL)20例及淋巴结反应性增生病人10例作为对比研究。③结果AIL中P53蛋白阳性表达率为30%(6/20),与预后无关。DNA含量改变对于病人预后有显著影响(t=2.864,P<0.05)。P53蛋白表达、DNA含量在AIL与NHL间差异均有显著性(χ2=4.912,t=3.552,P<0.05)。AIL中P53蛋白阳性表达者DNA含量显著高于阴性表达者(t=2.226,P<0.05)。④结论P53蛋白表达及DNA含量变化均与AIL发生发展及恶性转化有关,AIL可能为不同原因、不同性质的疾病共有的临床症候群。 相似文献
7.
该组研究采用免疫组化法对正常卵巢组织30例,良性上皮肿瘤30例,交界性肿瘤11例,性索间质瘤21例,上皮性癌40例,恶性生殖细胞瘤21例进行了抑癌基因P53蛋白表达的检测。P53蛋白表达阳性例数分别为0,1(3.3%),2(18.2%)。5(23.8%)。22(55%),23(61.9%)。结果显示:卵巢良性上皮肿瘤、交界性肿瘤和性索间质瘤。上皮性癌和恶性生殖细胞性肿瘤三组间P53蛋白阳性表达差异有显著性。(P<0.05) 相似文献
8.
采用分子原位杂交和SP法免疫组化技术对柳州地区40例食管鳞状细胞癌和20例食管粘膜慢性炎的材料进行地高辛标记的HPV6B/11、16、18DNA及HPV16、18早期蛋白E6、P21ras和P53癌基因产物检测,结果显示:食管癌中HPV16、18DNA及E6蛋白的阳性率分别为25%(10/40)和65%(26/40),与对照组对比差异均有高度显著性(P<0.01)。E6、和P53蛋白呈双阳性者为52.50%(21/40),其中90.48%(19/21)阳性表达出现在同一区域同一癌细胞核内,似表达E6、可与P53结合形成复合物,从而导致野生型P53的降解或突变。本组鳞癌组织中P21ras与P53、P53与E6的阳性表达均具有显著相关性(P<0.05)。提示高危HPV16、18感染与多癌基因协同在本地区食管癌病因学中起着十分重要的作用。 相似文献
9.
目的:探讨Ⅰ期子宫内膜癌p21ras 表达及DNA 含量。方法:ABC免疫组化法及流式细胞术。结果:随组织学分级增高p21ras表达率增高(P< 0.05),DNA 异倍体率亦增加(P< 0.05)。异倍体子宫内膜癌中p21ras阳性表达率(82.35% )较二倍体子宫内膜癌(30.77% )高(P< 0.05)。结论:提示p21ras作为子宫内膜癌预后参数的可能性,尤其是双参数同步分析将更好地评价子宫内膜癌预后 相似文献
10.
毛荣军 《右江民族医学院学报》1998,20(4):532-533
对51例膀胱移行细胞癌,9例正常膀胱粘膜及8例膀胱慢性炎组织,应用抗P53蛋白单克隆抗体进行S-P免疫组化染色。结果表明:非癌组织均无P53蛋白阳性表达,膀胱移行细胞癌阳性表达率为49.02%,且随分化程度的不同,阳性表达率有差异,0~Ⅰ级癌P53蛋白阳性表达率为19.05%,Ⅱ~Ⅲ级癌P53蛋白阳性表达率为70.00%,两者之间差异有显著性(P<0.05)。此外,有淋巴结转移的移行细胞癌(P53癌基因蛋白阳性表达率为69.5%)与无淋巴结癌转移者(P53癌基因蛋白阳性表达率为32.14%)两组之间差异有高度显著性(P<0.01)。上述结果提示检测P53癌基因蛋白的表达情况可能成为膀胱移行细胞癌分级、估计淋巴结转移及预后的一项重要参考指标 相似文献
11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD. 相似文献
12.
Jiageng Zhu Jun Li Ruipeng Ji Jianghao Su Mingshun Shen Zhigang Cao 《南京医科大学学报(自然科学版)》2007,21(2):125-128
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment. 相似文献
13.
Tao Ye * Zhi-quan Liu Jian-jun Mu Xi-han Fu Jun Yang Bao-lin Gao and Xiao-hong Zhang Department of Cardiology First Hospital of Xi'an Jiaotong University Xi'an Hanzhong Cardiovascular Institute Hanzhong 《中国医学科学杂志(英文版)》2004,19(4):248-251
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure. 相似文献
14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险. 相似文献
15.
Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu 《南京医科大学学报(自然科学版)》2007,21(6):386-389
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. 相似文献
16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption. 相似文献
17.
The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas: report of 9 cases 总被引:1,自引:0,他引:1
Shaoqin Chen Shengquan Zou Qibao Dai Peisheng Huang Weihua Cai Hong Li 《南京医科大学学报(自然科学版)》2007,21(6):398-401
Objective:To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas(SPTP). Methods:Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin(HE) staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results:Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin(9/9 cases), AAT(9/9 cases), NSE(9/9 cases), ACT(7/9 cases), CK20(2/9 cases), CgA(1/9 cases), S-100(3/gcases), PR(4/gcases), Syn(3/9 cases) and CD56(5/9cases), negative in CEA and ER. Conclusion:SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype. 相似文献
18.
Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine
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A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes. 相似文献
19.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P. 相似文献
20.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine. 相似文献