红外线对萎缩性胃炎大鼠PCNA及Bcl-2表达的影响

目的 观察红外线照射在大鼠慢性萎缩性胃炎（chronic atrophic gastritis,CAG）中对增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）和B细胞淋巴瘤/白血病-2（B-cell lymphoma-2,Bcl-2）表达的影响,探讨红外线与胃黏膜癌前病变、细胞凋亡的关系.方法 应用水杨酸钠和酒精的混合溶液灌胃,并结合劳累、饥饱失常等多因素建立大鼠CAG模型,采用红外线灯照射CAG大鼠,免疫组化法检测各组大鼠PCNA及Bcl-2的表达.结果 PCNA及Bcl-2在模型对照组胃黏膜中均呈高表达（P〈0.05）,但在红外线组均无显著降低.结论 红外线照射未能降低PCNA及Bcl-2 的表达,细胞的增殖调节改善作用不明显.     

He-Ne激光对慢性萎缩性胃炎大鼠胃黏膜的影响

为探讨He-Ne激光对慢性萎缩性胃炎(CAG)大鼠胃黏膜的影响,将52只成年雄性Wistar大鼠随机分成正常组、非治疗组及各激光治疗组。应用2%水杨酸钠和30%酒精的混合溶液灌胃8周,刺激大鼠胃黏膜,并结合劳累、饥饱失常等多因素方法建立大鼠CAG模型,各治疗组在造模完成后,分别给予不同剂量(6.24J·cm-2,4.80J·cm-2,3.36J·cm-2)的He-Ne激光照射,每日一次,连续20d,观察各组大鼠胃黏膜的变化。发现3.36J·cm-2的He-Ne激光组与非治疗组比较,胃黏膜变厚(P<0.01),炎细胞减少(P<0.05),细胞形态、结构、体积、均恢复或接近正常;CyclinD1的阳性表达率较高(P<0.05)。表明3.36J·cm-2的He-Ne激光照射能够促进CAG大鼠的胃黏膜功能恢复,疗效显著。     

正常雏鸡耳蜗基底乳头细胞增殖及表皮生长因子的促增殖作用

利用增殖细胞核抗原免疫组织化学技术观察了正常雏鸡了及注射表皮生长因子后耳蜗底乳头的细胞增殖。结果发现：正常雏基底乳头毛细胞无阳性反应，少部分支持细胞和透明产殖的细胞核抗原反应染色阳性，表皮生和茵子注射后其阳性细胞明显增多。     

浅谈慢性萎缩性胃炎

在临床诊治慢性萎缩性胃炎过程中,准确辩证是取得良好疗效的关键,抓住主证,在辨证用药的基础上,选加辨病治疗中药,中西结合,做到遵古而不泥古,并结合现代医学进展,根据患者具体情况灵活用药,才能取得满意疗效,同时还能使传统的中医学理论得以发扬。     

养胃冲剂对大鼠慢性萎缩性胃炎胃黏膜的保护作用

目的　观察养胃冲剂对慢性萎缩性胃炎组织病理学变化的影响。方法　施行胃 肠吻合术建立慢性萎缩性胃炎 ,连续灌胃给药 3个月后 ,大体观察并作石蜡切片 ,HE染色后在显微镜下观察并拍照。结果　养胃冲剂能明显改善胃上皮损伤程度、减少炎症浸润范围 ,缩小胃小凹的宽度 ,与模型组比较有明显差异 (P <0 0 5) ,与阳性对照组无差别 (P >0 0 5)。结论　养胃冲剂通过减轻胃黏膜炎症反应、逆转肠上皮化生能有效的修复损伤的胃黏膜 ,从而改善胃黏膜萎缩     

黄芪建中汤对脾虚型慢性萎缩性胃炎大鼠消化系统的影响

目的:探讨黄芪建中汤对慢性萎缩性胃炎(CAG)的疗效及其机制.方法:将24只Wistar大鼠随机分为正常对照组(C)、CAG组、维酶素(V)组、黄芪建中汤(HJD)组,观察大鼠胃黏膜、空肠黏膜、肝和胰外分泌部组织中的糖原含量、SDH、LDH、ANAE和ACP活性.结果:与正常大鼠相比,CAG组大鼠一般状况欠佳,体质量明显减轻;大鼠胃黏膜萎缩、变薄,胃黏膜炎症程度积分和胃黏膜糜烂程度积分明显升高,而胃黏膜厚度和腺管总长度明显降低;空肠和肝也呈现出不同程度的病理学改变;大鼠上述脏器的糖原含量、SDH、ANAE、ACP和LDH活性多数显著降低;经黄芪建中汤治疗21d后,上述指标明显改善并明显优于维酶素组.结论:脾虚型CAG状态下,机体存在多脏器代谢紊乱,黄芪建中汤对之有良好的复健作用,其疗效显著优于维酶素.     

正常雏鸡耳蜗基底乳关细胞增殖及表皮生长因子的促增殖作用

利用增殖细胞核抗原免疫组织化学技术观察了正常雏鸡及注射表皮生长因子后耳蜗基底乳头的细胞增殖。结果发现：正常雏鸡基底乳头毛细胞无阳性反应,少部分支持细胞和透明细胞增殖细胞核抗原反应染色阳性;表皮生长因子注射后其阳性细胞明显增多。提示正常雏鸡基底乳头支持细胞和透明细胞有低水平的细胞增殖,表皮生长因子有明显的促增殖作用。     

大鼠下颌下腺切除对肝再生的影响

目的：探讨大鼠下颌下腺切除后对肝再生的影响。方法：将21只雄性SD大鼠分成三组：3只正常大鼠作为对照组(N组)；9只行肝大部切除术(H组)；9只行肝大部切除术和双侧下颌下腺切除术(E十H组)。应用增殖细胞核抗原(PCNA)免疫组化和核仁组成区嗜银蛋白(AgNOR)染色方法，对N组，H组，E H组的肝组织进行检测和计量。结果：术后第1天及第3天E H组的PCNA指数、AgNOR数及残余肝脏重量均明显低于H组。结论：结果表明大鼠下颌下腺切除后，肝细胞增殖延迟，肝再行过程延缓，提示下颌下腺分泌的EGF对肝再生起重要调节作用。     

阿托伐他汀对移植动脉慢性排斥反应的抑制作用及机制

目的： 研究阿托伐他汀(atorvastatin)对大鼠移植动脉慢性排斥的抑制作用及机制。方法： 建立大鼠腹主动脉移植慢性排斥反应模型，分为3组：异系移植对照组、异系移植治疗组和同系移植组，60 d后对移植动脉行病理组织学检测，观察移植动脉内膜增生程度；并行免疫组织化学染色(SP法)，检测移植动脉增殖细胞核抗原（PCNA）和平滑肌肌动蛋白（α-SMA）的表达情况,行硝酸还原酶比色法测定移植动脉组织NO的含量。结果： 异系移植治疗组血管内膜增厚程度(11.60%±2.40%)显著低于异系移植对照组(34.60%±6.40%,P<0.05)，高于同系移植组（1.15%±0.65%,P<0.05）；PCNA在异系移植治疗组（4.80%±0.80%）表达明显低于异系移植对照组（18.40%±1.80%,P<0.05），高于同系移植组（1.20%±0.40%,P<0.05）。 结论： 阿托伐他汀可抑制大鼠移植动脉慢性排斥反应，PCNA基因表达的下调可能是阿托伐他汀抑制移植动脉慢性排斥反应的机制之一。     

Low-dose radiation modifies the progression of chronic renal failure

Previous studies have indicated that the application of low dose radiation to an arterial ligation has the potential to subsequently reduce or eliminate restenosis caused by smooth muscle cell proliferation. Sufficient kidney irradiation causes a radiation nephropathy and often leads to renal failure. In order to evaluate the effect of low-dose irradiation on the kidney we hypothesized that this particular therapy modifies renal injury in rats with renal ablation and subsequently slows the rate of the progression. For further clarification of the effect of irradiation at low doses, we determined proliferating cell nuclear antigen (PCNA) and monocyte chemoattractant protein-1 (MCP-1) expression in remnant kidneys after low-dose radiation. Adult Wistar rats (n = 10) were studied during the two weeks after renal ablation. The left kidney was irradiated 24 hours after an operation in anaesthetised animals with 3 Grey in a single dose. Ablated rats without irradiation (n = 9) served as nephrectomized animals group. Rats without surgery and without radiation (n = 10) served as healthy controls. Renal damage was assessed using the following parameters: urine protein excretion rate (UprotV, mg/day), awake systolic blood pressure (SBP, mm Hg), serum creatinine (SCr, micromol/l). The indirect immunofluorescence method was used for the detection of PCNA and MCP-1 expression. Glomerular and tubular immunostaining was scored semiquantitatively. Numerous PCNA positive cells and MCP-1 expression were present in the glomerulus and tubulointerstitium in nephrectomized rat kidneys. Low-dose radiation application was associated with a significant reduction in PCNA and low MCP-1 expression. This study shows that the application of low-dose irradiation has the potential to modify the progression of chronic renal failure in rats.     

ERK在慢性哮喘大鼠气道平滑肌细胞增殖中的作用

目的：观察细胞外信号调节蛋白激酶(ERK)在慢性哮喘大鼠气道平滑肌细胞的表达，以探讨ERK信号通路在气道平滑肌增殖中的作用。方法：病理图像分析慢性哮喘大鼠气道重塑，免疫组化法检测ERK和PCNA在肺内表达，激光共聚焦显微镜分析ERK1/2、磷酸化ERK1/2和PCNA在气道平滑肌的共表达，免疫印迹和原位杂交检测气道平滑肌中ERK和PCNA蛋白以及mRNA的表达。结果：慢性哮喘大鼠有气道平滑肌层增厚，出现结构重塑。ERK和PCNA在肺内表达增强，同时在气道平滑肌上有ERK和PCNA蛋白与mRNA表达增加。结论：ERK可能是介导慢性哮喘气道重建中平滑肌增殖的重要信号通路之一。     

乳腺叶状囊肉瘤的增殖细胞核抗原的表达及其意义

目的研究乳腺叶状囊肉瘤增殖细胞核抗原（ＰＣＮＡ）的表达及其临床病理意义。方法采用免疫组化ＳＰ法对１００例乳腺叶状囊肉瘤及３９例乳腺腺纤维瘤进行检测。结果叶状囊肉瘤中间质性瘤细胞呈ＰＣＮＡ阳性着色者８６例（８６％），不同组织学级别者ＰＣＮＡ指数之间有显著差异（Ｆ＝８５．３３，Ｐ＜０．０１），分化越差，ＰＣＮＡ平均指数越高，ＰＣＮＡ指数与组织学分级密切相关（ｒ′ｓ＝０．７７）。Ⅰ级叶状囊肉瘤与乳腺腺纤维瘤间质性瘤细胞丰富组的ＰＣＮＡ指数也有显著差异（ｔ＝３．４２，Ｐ＜０．０１）。ＰＣＮＡ指数与核分裂相计数间相关关系并不密切（ｒ＝０．３９）。结论用ＰＣＮＡ来反映乳腺叶状囊肉瘤增殖活性，辅助进行组织学分级，鉴别良恶交界性病变及估测预后，都具有一定的实用价值。     

结直肠癌、结直肠腺瘤微卫星不稳定性检测及其临床意义

目的 探讨微卫星不稳定性（ＭＳＩ）在结直肠癌发生中的意义及其与结直肠癌细胞增殖活性和预后的关系,方法 采用聚合酶链反应－简单序列长度多态性（ＰＣＲ－ＳＳＬＰ）及免疫组化ＳＰ方法,对５６例结直肠癌,９例腺瘤及６例腺瘤癌变的ＭＳＩ和增殖细胞核抗原（ＰＣＮＡ）表达进行了检测。结果 显示结直肠癌ＭＳＩ总阳性率为４４．６４％（２５／５６例）,共中遗传性非息肉病性结直肠癌３／４例阳性,散发性结直肠癌２２／５２     

增殖细胞核抗原阳性细胞在幼年大鼠前脑的分布

目的　探讨幼年大鼠前脑内是否存在增殖细胞。方法　用增殖细胞核抗原 (PCNA)单克隆抗体PC10免疫组化研究。结果　PCNA阳性反应部位分两类 :一类为圆形或卵圆形深染颗粒 ,具有核的形态和大小 ,主要分布于吻侧迁移流、室管膜下带和部分血管壁 ;散在分布于尾壳核、运动皮质、隔区和斜角带 ;有时见“镜影核” ,主要存在于尾壳核 ;另一类为整胞体染色 ,见于室管膜、室管膜下带和吻侧迁移流。结论　幼年大鼠前脑内存在增殖细胞     

Comparative proteomics analysis of chronic atrophic gastritis: changes of protein expression in chronic atrophic gastritis with out Helicobacter pylori infection

Chronic atrophic gastritis (CAG) is a very common gastritis and one of the major precursor lesions of gastric cancer, one of the most common cancers worldwide. The molecular mechanism underlying CAG is unclear, but its elucidation is essential for the prevention and early detection of gastric cancer and appropriate intervention. A combination of two-dimensional gel electrophoresis and mass spectrometry was used in the present study to analyze the differentially expressed proteins. Samples from 21 patients (9 females and 12 males; mean age: 61.8 years) were used. We identified 18 differentially expressed proteins in CAG compared with matched normal mucosa. Eight proteins were up-regulated and 10 down-regulated in CAG when compared with the same amounts of proteins in individually matched normal gastric mucosa. Two novel proteins, proteasome activator subunit 1 (PSME1), which was down-regulated in CAG, and ribosomal protein S12 (RPS12), which was up-regulated in CAG, were further investigated. Their expression was validated by Western blot and RT-PCR in 15 CAG samples matched with normal mucosa. The expression level of RPS12 was significantly higher in CAG than in matched normal gastric mucosa (P < 0.05). In contrast, the expression level of PSME1 in CAG was significantly lower than in matched normal gastric mucosa (P < 0.05). This study clearly demonstrated that there are some changes in protein expression between CAG and normal mucosa. In these changes, down-regulation of PSME1 and up-regulation of RPS12 could be involved in the development of CAG. Thus, the differentially expressed proteins might play important roles in CAG as functional molecules.     

环氧合酶-2抑制剂对盐酸诱导大鼠胃粘膜损伤后上皮细胞增殖和愈合的影响

目的:探讨特异性和非特异性环氧合酶-2(COX-2)抑制剂,对盐酸诱导胃粘膜损伤后上皮细胞增殖和损伤愈合的影响。方法:大鼠胃内给予06mol/LHCl1mL后,胃内给予NS-398或吲哚美辛,Westernblot和免疫组化法分析盐酸灌胃前、后胃粘膜COX-2表达,免疫组化检测增殖细胞核抗原(PCNA)评价上皮细胞的增殖状态,小格图纸法计算胃损伤指数(LI)。结果:盐酸灌胃后,COX-2在胃粘膜表层上皮细胞和腺颈部成体干细胞高表达。盐酸灌胃后24h,NS-39840mg/kg组及吲哚美辛组PCNA标记指数(PCNA-LI)分别为(22.72±4.33)%和(21.98±5.18)%,明显低于对照组(34.46±3.61)%(P<0.05);NS-3984mg/kg组和40mg/kg组LI分别为(1.28±0.58)%和(1.16±0.56)%,显著高于对照组(0.58±0.24)%(P<0.05)。结论:COX-2抑制剂抑制胃粘膜上皮细胞增殖,延迟大鼠胃粘膜损伤的愈合,提示COX-2表达在胃粘膜再生过程中起重要作用。     

胶质瘤增殖活性和肿瘤基因蛋白表达及其意义

目的探讨胶质瘤增殖活性和肿瘤基因蛋白表达与分化和预后间的关系。方法应用免疫组化和图像分析技术对１２４例脑胶质瘤增殖细胞核抗原（ＰＣＮＡ）和几种肿瘤基因蛋白进行定性、定量研究。结果ＰＣＮＡ反应强度与级别和预后显著相关;ｃ－ｅｒｂＢ－２蛋白表达在分化程度高的胶质瘤强于分化程度低者,存活５年以上的病例阳性强于存活５年以下者。ＥＧＦ（４０．０％）、ＥＧＦＲ（９１．４％）和ｐ２１ｒａｓ（５３．３％）与分级和预后无显著关系。３种ｐ５３蛋白抗体阳性率均以Ⅱ～Ⅳ级胶质瘤为高;ｐ５３与ＰＣＮＡ反应强度平行。结论ｐ２１ｒａｓ、ｃ－ｅｒｂＢ－２、ＥＧＦ和ＥＧＦＲ改变在胶质瘤发生、发展过程中可能是早期事件,恶变后不再随级别增加而加重,而ｐ５３基因突变涉及胶质瘤发展各个阶段;ＰＣＮＡ能较好地反映胶质瘤的恶性程度。