7日GnRHa短方案对卵泡液中IGF-Ⅱ和IGFBP-4浓度的影响

目的:探讨7日促性腺激素释放激素激动剂(gonadotropin releasing hormone agonist,GnRHa)短方案与GnRHa长方案对卵泡液中胰岛素样生长因子-Ⅱ(insulin-like growth factor Ⅱ,IGF-Ⅱ)和胰岛素样生长因子结合蛋白-4(insulin-like growth factor binding protein-4,IGFBP-4)浓度水平的影响.方法:将接受体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)治疗的88例输卵管因素不孕患者,随机分成7日GnRHa短方案组和GnRHa长方案组(n=44).收集取卵日优势卵泡的卵泡液,采用放射免疫法测定IGF-Ⅱ浓度,酶联免疫吸附法测定IGFBP-4浓度.结果:7日GnRHa短方案与长方案组相比较,Gn用量明显减少,用药时间明显缩短.两组间人绒毛膜促性腺激素(hCG)注射日血清雌二醇(E2)和每成熟卵泡E2的水平差异均无统计学意义(P＞0.05).7日GnRHa短方案卵泡液的IGF-Ⅱ和IGFBP-4水平明显低于GnRHa长方案组.两组间卵泡液的IGF-Ⅱ/ IGFBP-4比值差异无统计学意义(P＞0.05).卵泡液中的IGF-Ⅱ水平与Gn 用量呈显著正相关.结论:7日GnRHa短方案与长方案引起卵泡液中IGF-Ⅱ和IGFBP-4的浓度变化,但卵泡液中IGF-Ⅱ和IGFBP-4浓度的改变并未导致临床结局的不同.     

2型糖尿病患者治疗前后血清IGF-Ⅱ浓度改变及相关因素的研究

目的 :了解 2型糖尿病患者治疗前后血清 IGF- 水平。方法 :用放免测定法对正常对照组及 2型糖尿病患者血清 IGF- 水平进行检测。结果 :2型糖尿病患者空腹血清 IGF- 水平治疗前为 (0 .4 8± 0 .4 0 ) μg/ L、治疗后为 (0 .4 2± 0 .33) μg/L,比较无显著性差异 (P >0 .0 5 ) ,但与正常对照组 (0 .98± 0 .2 0 ) μg/ L 比较有显著性差异 (P <0 .0 1)。 2型糖尿病患者治疗前空腹血清 IGF- 浓度与空腹 C肽 (P <0 .0 5 )呈正相关。结论 :血糖控制对血清 IGF- 水平影响较小 ,但与正常对照组有显著差别     

吉西他滨-卡铂方案后续贯性应用紫杉醇-卡铂方案作为晚期卵巢癌一线治疗方案：一项Ⅱ期研究

目的:本文旨在对在吉西他滨-卡铂方案后续贯性应用紫杉醇-卡铂方案作为晚期卵巢癌一线治疗方案的可行性及效果进行研究,以患者的缓解率作为研究的主要终点。方法:56例FIG O III～IV期患者在开腹手术后,接受4周期的吉西他滨1000m g/m2(第1天,第8天)和卡铂AUC5(44例患者)或A U C6     

探讨宫颈癌患者血清中胰岛素样生长因子-Ⅱ水平的意义

目的:探讨宫颈癌患者血清中胰岛素样生长因子-Ⅱ(IGF-Ⅱ)水平与宫颈癌发生、预后及治疗疗效的关系.方法:抽取正常对照25例及宫颈癌患者29例治疗前后的肘静脉血,分离血清,采用放射免疫法检测标本中IGF-Ⅱ浓度并进行比较.结果:宫颈癌患者手术治疗前血清IGF-Ⅱ水平高于正常对照组,差异有显著性(P<0.01),手术治疗后3月血清IGF-Ⅱ水平明显下降,与术前比较差异有显著性(P<0.01),有淋巴结转移者的宫颈癌患者血清IGF-Ⅱ水平高于无淋巴结转移者,差异有显著性(P<0.01).结论:测定宫颈癌患者血清中IGF-Ⅱ水平的变化对宫颈癌患者的临床诊断、治疗疗效及预后可提供一定的有意义的参考价值.     

CPAP对伴OSA的慢性心衰患者临床观察

目的：观察经鼻持续气道正压通气（CPAP）对伴OSA的慢性心衰（CHF）患者的临床效果。方法将80例稳定期CHF合并有OSA患者随机分为两组各40例，实验组在常规治疗慢性心衰的基础上，应用CPAP呼吸机每晚进行7-9h治疗，持续6个月；对照组进行常规基础药物治疗。6个月后，用超声心动图检测患者左室射血分数（EF值）及左室舒张末内径（LVED），抽血采用免疫荧光快速诊断仪检测患者血浆B型脑钠肽（BNP）、血管紧张素（AngⅡ）、醛固酮（Ald）值。结果治疗6个月后，实验组LVEF值较对照组LVEF值明显升高，实验组LvDd、BNP、AngⅡ及Ald较对照组均明显降低，组间差异有统计学意义（P 〈0.05）。结论中重度OSA合并CHF患者在基础药物治疗基础上接受CPAP治疗有明显的额外收益，可能与降低心衰患者的BNP、AngⅡ及Ald有关。     

Effect of Sodium Tanshinone Ⅱ A Sulfonate on Phosphorylation of Extracellular Signal-regulated Kinase1/2 in Angiotensin Ⅱ-induced Hypertrophy of Myocardial Cells

Objective:To observe the effects of sodium tanshinone Ⅱ A sulfonate(STS)on angiotensin Ⅱ(Ang Ⅱ)-induced hypertrophy of myocardial cells through the expression of phosphorylated extracellular signal-regulated kinase(P-ERK1/2).Methods:In the primary culture of neonatal rat myocardial cells.the total protein content in myocardial cells was determined by coomassie brilliant blue and the protein synthesis rate was measured by[3H]-Leucine incorporation as indexes for hypertrophy of myocardial cells.The expression of p-ERK1/2 was determined using Western blot and immunofluorescence Iabeling.Results:(1)The totaI protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial cells were stimulated by Ang Ⅱ (1 μmol/L)for 24 h;STS markedly inhibited the increment of the total protein level induced by Ang Ⅱ and the syntheses of protein.(2)After pretreatment of myocardial cells with Ang Ⅱ(1 μ mol/L)for 5 min,the p-ERK1/2 protein expression was increased,with the most obvious effect shown at about 10 min;pretreatment of myocardial cells with STS at different doses(2,10,50 μ mol/L)for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated by Ang Ⅱ in a dose-dependent manner.(3)After the myocardial cells were stimulated by Ang Ⅱ(1 μ mol/L),the immunofluorescence of ERK1/2 rapidly appeared in the nucleus.The activation and translocation process of ERK1/2 induced by Ang Ⅱ was blocked distinctly by STS.Conclusion:STS inhibited the myocardial cell hypertrophy induced by Ang Ⅱ,and the mechanism may be associated with the inhibition of p-ERK1/2 expression.     

105例死亡病例SAPSⅡ及APACHEⅡ分析

目的比较简化急性生理学评分Ⅱ(SAPSⅡ)、急性生理学及慢性健康状况评分Ⅱ(APACHEⅡ)对内科死亡病例的评估效能。方法对105例内科危重症住院死亡病例进行SAPSⅡ及APACHEⅡ评分,比较两种评分系统死亡预测率。结果SAPSⅡ分值为55.89±19.57,其死亡预测率(PDR1)为54.67±27.67(%);APACHEⅡ分值为25.36±9.45,其校正死亡预测率(PDR2)为64.03±22.87(%);SAPSⅡ与APACHEⅡ明显相关(r=0.861,P<0.01);PDR2与PDR1明显相关(r=0.847,P<0.01);PDR2显著高于PDR1(P<0.01)。结论APACHEⅡ评分系统较SAPSⅡ评分系统更能预测死亡预后。     

萘哌地尔衍生物YMⅡ对血栓形成的影响

目的 观察萘哌地尔衍生物YMⅡ对大鼠血栓形成的影响。方法 采用动—静脉旁路血栓形成法的大鼠模型来观察YMⅡ对血栓形成的作用。结果 YMⅡ尾静脉给药可降低模型大鼠形成的血栓重量。结论 YMⅡ对大鼠血栓形成有抑制作用。     

HPLC法测定离体透皮接受液中丹参酮ⅡA的浓度△

建立了测定离体透皮接受液中丹参酮Ⅱ     

Effects of Qindan Capsule (芩丹胶囊) on blood pressure, endothelin, calcitonin gene-related peptide and angiotensin-II in spontaneous hypertensive rats

Objective： To observe the hypotensive effects of Qindan Capsule （芩丹胶囊, QC） on spontaneous hypertensive rats （SHR） and its effect on the contents of endothelin （ET）, calcitonin gene-related peptide （CGRP） and angiotensin-Ⅱ （Ang-Ⅱ ） in plasma and vascular tissues, and to investigate the possible mechanism of QC in lowering blood pressure. Methods： Forty SHRs were divided into 5 groups： the high dosage QC group [QCHD, 750 mg/（kg.d） ], the low dosage QC group [QCLD, 150 mg/（kgd） ], the Niuhuang Jiangya Pill group [牛黄降压丸,, NJP, 200 mg/（kg.d）], the Captopril group [ 15 mg/（kgd）]and the model group, 8 in each group. Meanwhile, a normal control group consisting of 8 Wistar-Kyoto （WKY） rats was set up also. All the rats were administered with medicine level of ET, CGRP and Ang-Ⅱ in plasma and Ang-Ⅱ rats after 12 weeks of treatment. Results： The leve through gastrogavage. Systolic blood pressure （SBP）, in tissues of mesenteric artery were detected in all the of SBP after treatment in the QCHD group was lower than that in the model group （ P〈0.01 ）, but with no significant difference as compared with that in the Captopril group and the NJP group （P〉0.05）. After treatment, the plasma level of ET was lower and CGRP higher than those in the model group （both P〈0.05）, and also higher than those in the NJP and Captopril group （both P〈0.05）. As for the content of Ang- Ⅱ , in mesenteric arterial tissues, it was lower in the QCHD group than that in the model group （ P〈0.05）, but in plasma, it showed no significant difference between the two groups （P〉0.05）. Conclusion： QC has a satisfactory hypotensive action on SHR rats, and its mechanism may be associated with the regulation on plasma vasoactive peptide and regional renin-angiotensin system.     

Inhibiting NF-κB increases cholesterol efflux from THP-1 derivedfoam cells treated with Angll via up-regulating the expression of ATP-binding cassette transporter A1

Objective:To study the role of nuclear factor-kappa B(NF- K B) in cholesterol efflux from THP-I derived-foam cells treated with Angiotensin Ⅱ (Ang Ⅱ ). Methods:Cultured THP-l derived-foam cells were treated with Ang Ⅱ or preincubated with tosyl-phenylalan inechloromethyl-ketone(TPCK) NF-K B inhibitor. The levels of activated NF-K B in the cells were examined by sandwich ELISA. Cellular cholesterol content was studied by electron microscopy scanning and zymochemistry via fluorospectrophotometer and cholesterol efflux was detected by scintillation counting technique. ABCAI mRNA and protein were quantified by RT-PCR and Western blotting. Results:Addition of TPCK to the cells before Ang Ⅱ stimulation attenuated the response of NF- K B p65 nuclear translocation induced by Ang Ⅱ and showed no peak in foam cells group and caused a reduction in cholesterol content and an increase in cholesterol effiux by 24.1%(P < 0.05) and 41.1%(P < 0.05) respectively, when compared with Ang Ⅱ group. In accordance, the ABCAl mRNA and protein were increased by 30% and 19%(P< 0.05) respectively, when compared with Ang Ⅱ group. Conclusion:Ang Ⅱ can down- regulate ABCAI in THP-l derived-foam cells via NF- K B, which leads to less cholesterol effiux and the increase of cholesterol content with the consequence of the promotion of atherosclerosis.     

Ⅱ型糖尿病人TSA—Ⅱ定量感觉障碍检测分析

糖尿病周围神经病变是糖尿病慢性并发症中最常见的并发症之一,其起病隐袭,早期没有症状不易发现,通过TSA-Ⅱ定量感觉障碍测定仪进行检测,早期发现周围神经病变,尽早治疗,以延缓、改善其病变.     

Effect of Ligustrazine on Activity Changes of Angiotensin Ⅱ in Plasma and Cerebrospinal Fluid in Patients with Vascular Dementia

Objective: To study the relationship between the activity changes of angiotensin II (Ang II ) in plasma, cerebrospinal fluid (CSF) and the pathophysiology of vascular dementia (VD) on the one hand and the therapeutic effects of ligustrazine (LIG) on VD and its mechanisms on the other hand. Methods: Case grouping: VD group with 50 cases (26 VD patients treated with LIG and 24 with Ginaton) ; cerebral infarction (CI) group with 62 cases (routine therapy was given) and control group (without organic disease in central nervous system) with 26 cases. Investigation method ? To test cognitive function by Mini-Mental State Examination (MMSE), Functional Activities Questionnaire (FAQ), Hachinski Ischemic Scale (HIS) and P300 peak latency (P300PL) . To measure the concentration of AngII by radioimmunoassay (RIA) technology. Results: As compared with CI recovery phase or with control group, AngII levels in CSF were markedly increased in VD group(P<0. 01), MMSE scores was significantly lowered (P<0. 01), P300PL was     

烧伤Ⅱ号喷涂治疗Ⅱ期压疮临床效果分析

目的探讨烧伤Ⅱ号喷涂治疗压疮的临床疗效。方法将100例压疮患者随机分为观察组（50例）和对照组（50例）。观察组采用烧伤Ⅱ号喷涂换药,每日2次;对照组采用传统方法治疗,每日2次。结果两组创面愈合时间、治疗总有效率比较差异有统计学意义（P〈0.01）。结论烧伤Ⅱ号有明显的活血化瘀、去腐生肌的功能,治疗效果明显,治愈率高,疗程短。值得推广。