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1.
Objective
To observe the effects of moxibustion pretreatment on the protein expressions of epidermal growth factor receptor (EGFR), phosphorylation extracellular signal-regulated kinase 1/2 (p-ERK1/2) and activated protein-1 (AP-1), the key factors of extracellular signal-regulated kinase signaling transduction pathway in gastric tissue of rats with stress-induced gastric mucosal damage, and to discuss the mechanisms of moxibustion therapy in promoting the restoration of damaged gastric mucosa.Methods
Thirty Sprague-Dawley (SD) rats were randomly divided into a normal group, a model group, and a moxibustion group using the random digits table, 10 in each group. Except the rats in the normal group, rats in the other two groups were used to make stress-induced gastric mucosal damage model using restraint and cold stress. Before modeling, rats in the moxibustion group were alternately treated with moxibustion at Zusanli (ST 36) and Zhongwan (CV 12), or Pishu (BL 20) and Weishu (BL 21), once a day, for a total of 8 d. Histological changes of gastric mucosa were observed under the light microscopy, the expression of gastric tissue p-ERK1/2 was detected by immunohistochemistry assay, and the protein levels of EGFR and AP-1 were measured by Western blots.Results
Compared with rats in the normal group, gastric mucosal damage was more serious, and protein expressions of gastric tissue EGFR, p-ERK1/2 and AP-1 increased in the model group (P<0.01, P<0.05, P<0.05). Compared with rats in the model group, gastric mucosal damage was milder, and protein expressions of gastric tissue EGFR, p-ERK1/2 and AP-1 increased in the moxibustion group (all P<0.01).Conclusion
Moxibustion at Zusanli (ST 36), Zhongwan (CV 12), Pishu (BL 20) and Weishu (BL 21) could increase EGFR, p-ERK1/2 and AP-1 expression levels in gastric tissue of stress-induced gastric mucosal damage rats, maintain the information transfer function of ERK signaling transduction pathway, and promote restoration of gastric mucosal damage.2.
Objective
To investigate the protective effect of moxibustion in initiating the endogenous protection information on gastric mucosa, and its relationship with the pathway of common peroneal nerve.Methods
Forty-eight Sprague-Dawley (SD) rats were randomly divided into a normal group (group A), a model group (group B), a moxibustion model group (group C) and a moxibustion model plus surgery group (group D), 12 in each group. Except for group A, rats in the other groups were treated with dehydrated ethanol and aspirin to prepare gastric mucosal damage model. The rats in group B were not treated with any interventions; rats in group C received moxibustion at Zusanli (ST 36), twice a day for continuous 3 d. The rats in group D were subjected to preparing the gastric mucosal damage model after the common peroneal nerve transection, followed by moxibustion at Zusanli (ST 36). After a 3-day intervention, ulcer index (UI) in each group was observed, and the levels of gastric mucosa-related repair cytokines of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4) and heat shock protein 70 (HSP70) were detected.Results
Compared with group A, the pathological changes and UI of group B were worse (P=0.000), but TNF-α in serum and tissue was changed significantly (P=0.000, P=0.002), IL-4 in serum and tissue was improved significantly (P=0.000, P=0.000). Compared with group B, TNF-α and IL-4 in group C and group D were significantly improved (TNF-α: P=0.003, P=0.016; IL-4: P=0.000, P=0.002). Compared with group C, the changes of UI in group B and group D were poor (both P=0.000); the levels of TNF-α and IL-4 in serum were significantly decreased (TNF-α: P=0.000, P=0.025; IL-4: P=0.000, P=0.034); and tissue HSP70 levels were decreased significantly (P=0.000, P=0.033).Conclusion
Zusanli (ST 36) can transmit information through the pathway of common peroneal nerve, regulate the release of gastric mucosal protective factors, and up-regulate the expression of cytothesis-related proteins, so as to achieve the effect in repairing gastric mucosa.3.
Objective
To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome.Methods
Seventy-five SPF grade Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a 3 moxa-cone ginger-partitioned moxibustion group (group C1), a 6 moxa-cone ginger-partitioned moxibustion group (group C2) and a 9 moxa-cone ginger-partitioned moxibustion group (group C3) using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 °C 200% concentrated Da Huang (Radix et Rhizoma Rhei) to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12) respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay (ELISA). The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry.Results
After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased (all P<0.01); compared with group B, the spleen deficiency scores were decreased in group C1, C2 and C3, and the serum levels of TFF1 and MUC5AC, as well as EGFR protein expression in gastric tissues were increased (all P<0.01). Compared with group C1, the spleen deficiency scores were decreased in group C2 and C3, the serum levels of TFF1 and MUC5AC, and the expression of EGFR protein in gastric tissues were increased (all P<0.01), however, there was no significant difference between group C2 and C3 (all P>0.05). The mechanism may be related to the increase of serum TFF1 and MUC5AC levels and activation of EGFR protein.Conclusion
Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone ginger-partitioned moxibustion groups.4.
Objective
To observe the effect of herbal cake-partitioned moxibustion on the expressions of mitogen-activated protein kinase (MEK1/2) and extracellular regulatory protein kinase (ERK1/2) in gastric tissues of rats with spleen deficiency syndrome, and to explore the possible mechanisms of herbal cake-partitioned moxibustion in treating spleen deficiency syndrome.Methods
Sixty Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a ranitidine group (group C), and a herbal cake-partitioned moxibustion group (group D) by random digit, 15 rats in each group. Rat models of spleen deficiency syndrome were made by intragastric administration of 4 °C 200% concentrated Da Huang (Radix et Rhizoma Rhei). After successful modeling, the rats in group C were treated with 25 mg/(kg·bw) ranitidine by intragastric adminstration and rats in group D were treated with herbal cake-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12), for 8 d. Excepted for rats in group A, all the other rats were treated with indomethacin at 5 mg/(kg·bw) at 8:00 a.m. on the second day after finishing all the intervention and sacrificed 7 h later to isolate the stomach. Histopathological changes of the gastric tissues were observed under light microscope after hematoxylin-eosin (HE) staining. The protein expressions of MEK1/2 and ERK1/2 in the gastric tissues were detected by immunohistochemistry.Results
After intervention, the gastric mucosal injury in group B was significantly severer than that in group A, with large breakage and ablating; the damage of gastric mucosa was decreased in group C compared with group B; the gastric mucosal surface remained relatively complete, and the status of breakage and ablating was significantly improved. After intervention, compared with group A, the protein expressions of MEK1/2 and ERK1/2 in gastric tissues of the other groups were significantly higher (P<0.01). Compared with group B, the protein expressions of MEK1/2 and ERK1/2 in group C and D were significantly higher (all P<0.01). Compared with group C, the protein expressions of MEK1/2 and ERK1/2 in group D were significantly higher (P<0.01).Conclusion
Herbal cake-partitioned moxibustion promotes the repair of gastric mucosa in rats with spleen deficiency syndrome, via improving protein expressions of MEK1/2 and ERK1/2 in gastric tissues, as well as activating MEK/ERK signaling pathway.5.
Objective
To investigate the efficacy and mechanisms of moxibustion-based treatment of chronic gastritis (CG), and to provide an objective basis for treating CG using moxibustion.Methods
A total of 61 CG patients were divided into an herbal cake-partitioned moxibustion group and a mild-warm moxibustion group. In both treatment groups, bilateral Tianshu (ST 25), Zhongwan (CV 12) and Qihai (CV 6) were selected for moxibustion. Before and after treatment, all the enrolled patients’ gastrointestinal disease-related traditional Chinese medicine (TCM) syndrome scores and visual analog scale (VAS) scores were measured, and the changes in the serum levels of the brain-gut peptides ghrelin, somatostatin (SS) and motilin (MTL) were observed.Results
There was no statistically significant difference between the two groups in the clinical efficacy rate (P>0.05). After treatment, the gastrointestinal disease-related TCM syndrome scores and VAS scores were reduced to varying extents in both groups, the intra-group differences were statistically significant (all P<0.01). In both groups, the serum levels of ghrelin and MTL increased and the serum levels of SS decreased after treatment (all P<0.01). And there were no serious adverse events occurred.Conclusion
Both herbal cake-partitioned moxibustion and mild-warm moxibustion are effective for CG; these two therapies exhibited similar therapeutic efficacy of epigastric discomfort or pain. And both the two therapies act to anti-inflammation, promote the recovery of gastric mucosa and improve the gastric motility, which is possibly their crucial action mechanism in treating CD.6.
Objective
To explore the correlation between moxibustion sensation and distance of moxa stick and provide reference for clinical practice.Methods
A total of 16 healthy volunteers aged 18-35 years old in college were recruited and given mild moxibustion at Shousanli (LI 10), Zusanli (ST 36), Shenshu (BL 23) and Tianshu (ST 25) with moxa stick, and the occurrence and frequency of moxibustion sensation were recorded at distances of 5 cm, 4 cm, 3 cm and 2 cm. Mild moxibustion scale was used to count the score.Results
Warm was the main moxibustion sensation, burning pain and soreness decreased with the rise of distance; for the same acupoint, score of mild moxibustion scale increased with the decrease of distance; score ranged between 5.5 and 6.5 at distance 3 cm, which was the most comfortable distance for volunteers.Conclusion
The distance of 3 cm is the most comfortable distance in mild moxibustion7.
Objective
To assess the effects of direct moxibustion on 24-hour ambulatory blood pressure (ABP) and clinical symptoms of traditional Chinese medicine (TCM) in elderly patients with essential hypertension, and to explore the antihypertensive effect and influencing factors of moxibustion.Methods
A total of 101 elderly hypertension patients who met the inclusion criteria were randomly assigned to a direct moxibustion I group (n=33), a direct moxibustion II group (n=34), and a control group (n=34). The treatment of calcium antagonist (CCB) or angiotensin II receptor antagonist (ARB) was adopted in the control group. The treatment of direct moxibustion I plus the same medicine as the control group were adopted in the direct moxibustion I group, five cones per acupoint and three times per week, for 5 weeks in total. The treatment of direct moxibustion II plus the same medicine as the control group were adopted in the direct moxibustion II group, five cones per acupoint and three times per week, for 5 weeks in total. The changes of 24-hour ABP and clinical symptoms of TCM after treatment were compared in the three groups.Results
The mean 24-hour ambulatory systolic blood pressure (mean 24 h ASBP), night ASBP, percentage of mean 24-hour ambulatory diastolic blood pressure (mean 24 h ADBP)>90 mmHg, and percentage of day ADBP>90 mmHg in the control group were elevated after treatment (P<0.05). The percentage of night ADBP>80 mmHg in the direct moxibustion I group was reduced by treatment (P<0.01). There were no significant differences in the other outcome measures of 24 h ABP, such as day ASBP, percentage of mean 24 h ASBP>140 mmHg, percentage of day ASBP>140 mmHg, percentage of night ASBP>120 mmHg, mean 24 h ADBP, day ADBP, night ADBP, 24 h ambulatory pulse pressure (APP), after treatment in all groups (P>0.05). The degree of improvement of the clinical symptoms of TCM showed significant differences among the three groups of patients (P<0.01). The total effective rate in the direct moxibustion I group was 73.3%, which was superior to those in the direct moxibustion II group and control group (13.3% and 10.0%, respectively).Conclusion
The direct moxibustion has benign regulative effect on blood pressure of elderly patients with essential hypertension, and improves their clinical symptoms. The direct moxibustion method I (burning the next moxa cone after the previous one had totally burnt out) was superior to method II (burning the next moxa cone when the previous one had not totally burnt out ) in lowering blood pressure and improving symptoms of elderly patients with essential hypertension.8.
Objective
To observe the clinical efficacy of warm needling moxibustion plus flash cupping for remission-stage peripheral facial paralysis (FP) due to wind-cold.Methods
Fifty eligible patients were randomized into a warm needling moxibustion group and an acupuncture-cupping group, 25 cases in each group. The warm needling moxibustion group was intervened by acupuncture at Fengchi (GB 20), Yangbai (GB 14) towards Yuyao (EX-HN 4), Xiaguan (ST 7), Dicang (ST 4) towards Jiache (ST 6), Quanliao (SI 18), and Hegu (LI 4), plus warm needling moxibustion at Quanliao (SI 18); the acupuncture-cupping group received flash cupping on the affected side in addition to the intervention given to the warm needling moxibustion group. The two groups were both treated once a day, 10 times as a treatment course, for 3 courses in total. The House-Brackmann (H-B) facial nerve grading system was observed before and after the intervention to evaluate the facial nerve function in the two groups, and the therapeutic efficacies were also compared between the two groups.Results
The two treatment protocols both can promote the recovery of facial nerve function. The total effective rate was 92.0% in the acupuncture-cupping group versus 72.0% in the warm needling moxibustion group, and the between-group difference was statistically significant (P<0.05).Conclusion
Warm needling moxibustion plus flash cupping can produce a more significant efficacy than dry warm needling moxibustion in treating remission-stage peripheral FP due to wind-cold.9.
目的:观察艾灸对溃疡性结肠炎大鼠结肠角质细胞生长因子-1 (Keratinocyte Growth Factor-1,KGF-1)、角质细胞生长因子-2(Keratinocyte Growth Factor-2,KGF-2) KGF-2和白介素-6(Interleukin-6,IL-6)蛋白表达的影响,进一步探讨艾灸治疗溃疡性结肠炎的作用机制.方法:将SD大鼠随机分为正常组、模型组、隔药灸组和柳氮磺胺吡啶(Sulfasalazine,SASP)组.隔药灸组采用隔药灸天枢和气海穴治疗;SASP组采用柳氮磺胺吡啶灌胃干预.干预结束后,应用HE染色光镜下观察各组大鼠结肠病理学变化,采用免疫组织化学法检测大鼠结肠KGF-1、KGF-2和IL-6蛋白表达变化.结果:与模型组比较,隔药灸组和SASP组大鼠结肠病理学均有一定的改善;与正常组比较,模型组大鼠结肠KGF-1、KGF-2和IL-6蛋白表达均显著增加(P＜0.05);经隔药灸和SASP干预后,大鼠结肠KGF-1、KGF-2和IL-6蛋白表达均显著降低(P＜0.05).结论:隔药灸和SASP均能下调溃疡性结肠炎大鼠结肠KGF-1、KGF-2和IL-6的蛋白表达,该作用可能是隔药灸和SASP治疗溃疡性结肠炎的重要机制之一. 相似文献
10.
目的：观察艾条悬灸对高脂血症患者生化指标的影响。方法：针对高脂血症患者,采用悬灸神阙和双侧足三里穴治疗,观察治疗前和治疗后空腹血糖（Fasting Blood Glucose,FBG）、甘油三酯（Triglyceride,TG）、总胆固醇（Total Cholesterol,TC）、高密度脂蛋白胆固醇（High Density Lipoprotein Cholesterol,HDL-C）、低密度脂蛋白胆固醇（Low Density Lipoprotein Cholesterol,LDL-C）的变化。结果：治疗后 FBG、TC、LDL-C 下降（P〈0.01）;TG 下降（P〈0.05）;HDL-C 治疗前后无统计学差异（P〉0.05）。结论：悬灸治疗高脂血症不仅能改善血脂代谢,同时还可以调节糖代谢。 相似文献
11.
目的:分析近5年美国《科学引文索引》(Science Citation Index,SCI)收录发表的艾灸文献,从而掌握当前艾灸研究近况,以期为本疗法的后续研究提供参考.方法:电子检索PubMed及Web of Science数据库,两位评价者独立整理检索文献,并对文献分别从数量、文献类型、期刊来源、影响因子(Impact Factor,IF)、国别、发表院校、病种及疗效评价和国内外比较八方面进行分析.结果和结论:共纳入文献53篇.近5年SCI收录艾灸文献逐年递增,多为临床研究或系统评价,但发表杂志IF不高(大多小于5);艾灸治疗病重繁多,疗效尚不确定,疗效机制也尚不明确.期待更多高质量的研究以明确艾灸临床疗效和增加相关文献的SCI发表数量. 相似文献
12.
Objective
To observe the effect of different moxibustion durations on rats with rheumatoid arthritis (RA) and to evaluate the relationship between moxibustion amount and moxibustion efficacy.Methods
Eight rats were randomly selected as a normal group from the 40 male Sprague-Dawley (SD) rats, and the other 32 rats were used to establish type II collagen-induced RA models. After successful modeling, the 32 rats were randomly divided into a model group, a moxibustion for 20 min group, a moxibustion for 40 min group and a moxibustion for 60 min group, with 8 rats in each group. Rats in the normal group did not receive modeling and moxibustion intervention; rats in the model group did not receive moxibustion after modeling; rats in the moxibustion for 20 min group, the moxibustion for 40 min group and the moxibustion for 60 min group were treated with moxibustion at Shenshu (BL 23) and Zusanli (ST 36) for 20 min, 40 min and 60 min, respectively. Six days were a course of treatment, with a total of 3-course treatments and a 1-day rest between the courses of treatment. After treatment, the serum levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α, arthritis index (AI) scores, toe volumes and pathological score of synovitis were evaluated in the rats.Results
Compared with the normal group, the serum IL-1β and TNF-α levels, and the toe volumes in the model group were increased, and the differences were statistically significant (P<0.01), before the treatment. Compared with the model group, the serum IL-1β and TNF-α levels, toe volumes and arthritis index (AI) scores were significantly decreased in the moxibustion for 20 min group, the moxibustion for 40 min group and the moxibustion for 60 min group (P<0.05 or P<0.01 ). Compared with the moxibustion for 20 min group and the moxibustion for 60 min group, serum IL-1β and TNF-α levels, toe volumes and AI scores were decreased more significantly in moxibustion for 40 min group, and the differences were statistically significant (P<0.05 or P<0.01). There were no significant differences in serum IL-1β and TNF-α levels, AI scores and toe volumes between the moxibustion for 20 min group and the moxibustion for 60 min group (all P>0.05). The synovial histopathological improvement was the most obvious in the moxibustion for 40 min group, when the synovial histopathological changes were compared among the moxibustion for 20 min group, moxibustion for 40 min group and moxibustion for 60 min group.Conclusion
The therapeutic efficacy of moxibustion for 40 min in RA rats was more significant than that of moxibustion for 20 min and moxibustion for 60 min, indicating that the duration of moxibustion is the main factor affecting its therapeutic efficacy.13.
Objective
To observe the effects of different moxibustion times on proteins of transient receptor potential vanilloid 3 (TRPV3) ion channel protein and synovial cell apoptosis in rats with rheumatoid arthritis (RA), to provide a new basis for the anti-inflammatory mechanism of moxibustion.Methods
A total of 50 Sprague-Dawley (SD) rats were divided into a normal group, a model group, moxibustion group I, moxibustion group II and moxibustion group III by complete randomization, with 10 rats in each group. Rats in the normal group were bred routinely, and rats in the model group were also bred routinely after successful modeling. After successful modeling, rats in moxibustion group I, II and III accepted consecutive moxibustion at Zusanli (ST 36) and Shenshu (BL 23) for 15 d, once a day, respectively 5 min, 20 min and 30 min for each session. The degree of paw edema was observed and recorded. Immunohistochemical assay was used to detect the protein expression of TRPV3 ion channel in dorsal root ganglia and spinal cord dorsal horn. Terminal-deoxynucleoitidyl transferase-mediated nick end labeling (TUNEL) was used to detect apoptotic synovial cell number.Results
At the end of treatment, paw circumference of rats in moxibustion group II and III were significantly reduced as compared with that in the model group (P<0.05). TRPV3 ion channel protein expression of dorsal root ganglia and spinal cord dorsal horn was higher in the model group than that in the normal group (P<0.05); the TRPV3 ion channel protein expressions of dorsal root ganglia and spinal cord dorsal horn in moxibustion group II and III were higher than that in moxibustion group I (P<0.05); apoptotic synovial cell number in the model group was larger than that in the normal group (P<0.05), and apoptotic synovial cell numbers in moxibustion group II and III were significantly higher than that in the model group (P<0.05).Conclusion
Moxibustion of appropriate time could induce TRPV3 expression, and promote synovial cell apoptosis.14.
艾灸对应激性胃溃疡大鼠胃粘膜细胞增殖和凋亡的影响及其与热休克蛋白表达关系的研究 总被引：1，自引：0，他引：1
目的:观察艾灸“足三里”等穴对应激性胃溃疡大鼠胃粘膜细胞增殖及凋亡的影响,分析热休克蛋白70基因表达(HSP70 mRNA)与上述效应的关系,探讨艾灸促进胃粘膜损伤修复的细胞分子生物学机制。方法:60只健康SD大鼠随机分为4组,即束缚对照组、模型组、艾灸“足三里”-“梁门”穴组和艾灸非穴对照点组。束缚水浸应激法制备胃溃疡模型,采用放射免疫方法测定胃粘膜转化生长因子(TGFα-)的含量,RT-PCR法测定HSP70 mRNA,免疫组织化学方法检测胃粘膜增殖细胞核抗原(PCNA)及细胞凋亡指数。结果:束缚水浸应激法造模后可致胃粘膜损伤指数升高,胃粘膜TGFα-含量下降,PCNA下降,细胞凋亡指数、HSP70 mRNA增加(P<0.05或P<0.01)。艾灸“足三里”“梁门”可降低胃粘膜损伤指数,增加胃粘膜TGFα-含量,促进HSP70 mRNA和PCNA的表达,降低胃粘膜细胞凋亡指数,与模型组和对照组比较有显著性差异(P<0.05或P<0.01)。结论:艾灸“足三里”“梁门”对应激性胃溃疡的胃粘膜具有保护作用,其机制可能是促进了TGFα-合成,刺激胃粘膜细胞增殖,抑制细胞凋亡,而这一过程可能与艾灸诱导HSP70表达有关。 相似文献
15.
目的:探讨艾灸血浆对胃黏膜细胞凋亡的影响,揭示艾灸对胃黏膜细胞的保护作用及其信号转导机制。方法:24名健康人随机等分为艾灸穴位组和艾灸非穴位组,分别艾灸中脘、关元和足三里穴及非穴位对照点各10 d。将人体胃黏膜上皮(GES-1)细胞分为空白组、模型组、艾灸穴位血浆组、艾灸非穴位血浆组,采用含8%乙醇的培养液造成GES-1细胞损伤模型,分别加以预先提取的灸前人体血浆、艾灸穴位和非穴位血浆。流式细胞技术检测细胞凋亡率;Western-blot法检测细胞内热休克蛋白70(HSP70)、第二个线粒体来源的胱氨酸酶激活物(Smac)、凋亡诱导因子(AIF)的表达;细胞免疫化学法检测半胱氨酸天冬酸蛋白酶-3、9(Caspase-3、9)的表达。结果:模型组与空白组比较,细胞凋亡率明显增加,HSP70、Smac、AIF、Caspase-3、Caspase-9表达均上调(均P<0.01);艾灸穴位血浆组与模型组比较,凋亡率明显下降(P<0.01),HSP70表达进一步提高(P<0.01),Smac、AIF、Caspase-3、Caspase-9表达下调(均P<0.01);艾灸非穴位血浆组与模型组比较,凋亡率、Smac表达下降,Caspase-3、Caspase-9表达也下调(均P<0.01),但高于艾灸穴位血浆组(P<0.05,P<0.01)。结论:艾灸中脘、关元和足三里穴位后提取的人体血浆能抑制GES-1细胞凋亡,其信号转导通路可能是:促进细胞内HSP70合成,通过线粒体凋亡通路,抑制Smac、AIF表达,同时阻断其与Caspase-9的结合,减少Caspase-3含量,抑制细胞凋亡。 相似文献
16.
Hyejung Park Hyeunggeug Kim Sara Yoo Namhun Lee Changgue Son 《Journal of traditional Chinese medicine》2012,32(4):590-595
Objective
Moxibustion has long been thought to promote vital energy or immunity through clinical experience. This study aimed to investigate the clinical effects of indirect moxibustion on antioxidant and immunomodulation in a normal population.Methods
Twenty-five healthy volunteers with no objective or subjective disorders were recruited. The participants were treated with indirect moxibustion on acupoints Guanyuan (CV 4) and Shenque (CV 8) three times per week for 4 weeks. The serum levels of reactive oxygen species (ROS) and malondialdehyde (MDA), the total antioxidant capacity (TAC), the activities of catalase and superoxide dismutase (SOD), and the total glutathione content were determined before and after the 12th moxibustion. Lymphocyte subpopulations and 42 cytokines in the peripheral blood were analyzed using flow cytometry and antibody array, respectively.Results
Compared with the initial time point, the serum levels of ROS and MDA were significantly lowered by moxibustion, while TAC was increased (P<0.01 for all). A significant increase was observed in catalase activity (P<0.05), but not in SOD or total glutathione. There were no significant changes in lymphocyte subpopulations or cytokines in the peripheral blood. Fifteen of 25 participants reported at least one symptom in which they felt subjective improvement after moxibustion.Conclusion
Indirect moxibustion on acupoints CV 4 and CV 8 improved the antioxidant defense system, which may be a mechanism explaining the clinical effects of moxibustion. 相似文献17.
龙海燕 《针灸推拿医学(英文版)》2017,15(6):420-425
Objective
To observe the effect of heat-sensitive moxibustion plus psychological intervention on serum contents of substance P (SP) and 5-hydroxytryptamine (5-HT), as well as the quality of life (QOL) in patients with irritable bowel syndrome (IBS).Methods
A total of 120 IBS patients were divided into three groups by random sampling method, 40 cases in each group. The heat-sensitive moxibustion group received heat-sensitive moxibustion, the psychological intervention group received psychological intervention, while the observation group received both methods. The therapeutic efficacy was evaluated after 3 courses of treatment.Results
After treatment, the therapeutic efficacy of the observation group was better than that of the heat-sensitive moxibustion group and the psychological intervention group (P<0.01), and the heat-sensitive moxibsution group was better than the psychological intervention group (P<0.05). In comparing the bowel symptom scale (BSS), the observation group was better than the other two groups (P<0.05), and there was a significant difference between the heat-sensitive moxibustion group and the psychological intervention group (P<0.05). In the comparison of QOL, the observation group was significantly superior to the other two groups (P<0.05), and there was a significant difference between the heat-sensitive moxibustion group and psychological intervention group (P<0.05). In comparing serum SP and 5-HT, the observation group was markedly better than the other two groups (P<0.05), and there were significant differences between the heat-sensitive moxibustion group and psychological intervention group (P<0.05). The self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were used to estimate the mental state of the three groups, revealing that the observation group was better than the heat-sensitive moxibustion group and the psychological intervention group (P<0.05), and there were significant differences between the latter two groups (P<0.05).Conclusion
Heat-sensitive moxibustion plus psychological intervention can regulate the levels of SP and 5-HT, improve BSS, SDS, SAS and QOL in treating IBS.18.
Objective
To categorize and summarize the clinical and mechanism studies of the past 30 years on the treatment of Hashimoto’s thyroiditis (HT) with moxibustion, moxibustion plus medication, and acupuncture plus medication, etc., and to analyze the current problems.Methods
The clinical and laboratory studies related to the treatment of HT with acupuncture-moxibustion therapies published before June 2015 were retrieved from MEDLINE, Excerpta Medica Database (EMBASE), China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full-text Database (Wanfang) and Chongqing VIP Database (CQVIP).Results
Moxibustion, moxibustion plus medication, and acupuncture plus medication can produce certain therapeutic effects in treating HT.Conclusion
The research on the treatment of HT with acupuncture-moxibustion therapies is rather limited in the amount and content. In the future, standardization should be fortified, specific moxibustion research needs deepening, and the action mechanism of moxibustion should be emphasized.19.
Zhu Shi-peng Luo Li Sun Zhi-fang Ding Na Zhang Qing-qing He Yu-wei Zhang Lu-fen Li Xiao-hong FengXiao-ming 《针灸推拿医学(英文版)》2013,11(4):199-203
Objective:To observe the changes of hypothalamus, ovarian leptin levels and blood lipid in 12-month-old rats with natural menopause, and the effect of preventive acupuncture and moxibustion at Guanyuan (CV 4). To explore the impact of preventive acupuncture and moxibustion on the internal environment of early menopause disorder. Methods:Menopausal female rats were screened by vaginal smear. Rat model of menopause was obtained by natural aging method. Preventive acupuncture and moxibustion at Guanyuan (CV 4) was used as intervention methods. Hypothalamus and ovarian leptin levels were detected by radioimmunoassay. Plasma total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL) levels were determined by biochemical methods. Results:Compared with the young control group, leptin levels in the hypothalamus and ovarian increased (P<0.01), plasma TC increased (P<0.05), LDL increased (P<0.01), and HDL reduced in 12-month-old rats. Hypothalamus and ovary leptin levels in 12-month-old rats with preventive acupuncture and moxibustion were significantly decreased (P<0.01); plasma TC and LDL levels decreased and HDL levels were increased in rats with preventive acupuncture (P<0.05). Conclusion:Preventive acupuncture and moxibustion can improve leptin resistance state and restore the abnormal lipid levels in menopausal body, and therefore can slow down the disorder of inner environment in early menopause. 相似文献
20.