电针调控大鼠胃酸分泌的机制

目的　探讨电针足三里穴对大鼠胃酸分泌的调控机制 .方法　 75只 SD大鼠随机分为 5组 ,在清醒状态下针刺双侧足三里穴 ,以空白对照、非经非穴作对照 ,三阴交穴作经穴对照 .同步测定胃液量、p H值及胃液酸度 ,放免法测定血浆、胃液、胃粘膜胃泌素 (Gas)和表皮生长因子 (EGF)含量 ,透射电镜观察胃粘膜腺细胞超微结构 .结果　电针刺激足三里穴后大鼠空腹胃液量明显减少 [(0 .30± 0 .11) vs(0 .6 3± 0 .12 )m L,P<0 .0 1],胃液酸度明显降低 [(30± 3) vs(4 1± 1)mmol· L- 1 ,P<0 .0 5 ],胃液 p H升高 .胃粘膜 Gas含量增多[(136± 17) vs(73± 4) ng· L- 1 ,P<0 .0 1],血浆 Gas含量下降 [(84± 2 2 ) vs (10 8± 30 ) ng· g- 1 ,P<0 .0 1],胃液 Gas无变化 ;胃液及胃粘膜 EGF含量显著升高 [(3.2± 1.1) vs(1.8± 0 .4) μg· L- 1和 (5 .1± 0 .8) vs(2 .4± 0 .4) μg· g- 1 ,P<0 .0 1],血浆 EGF无变化 .胃壁细胞泌酸小管不扩张 ,小管泡充盈不足 ,且数量不减少 ,非经非穴组及空白对照组各项指标无显著变化 .结论　电针足三里穴抑制大鼠胃 G细胞Gas释放 ,降低血浆 Gas,增加胃粘膜、胃液 EGF含量 ;电针足三里可改变胃壁细胞超微结构 ,抑制胃壁细胞泌酸小管功能、降低胃酸分泌     

纳络酮和西咪替丁对电针大鼠足三里胃酸分泌效应的影响

目的　探讨电针调节胃酸分泌的机制 .方法　采用精密酸度计测定电针后空腹胃液 p H值 ,滴定法测定胃液酸度 ,放免法测定胃液、血浆、胃粘膜及颌下腺 Gas和 EGF含量 .结果　注射纳络酮大鼠胃液酸度较生理盐水组有明显提高 (35 .3± 1.7) vs (2 6 .8± 2 .0 ) mmol· L- 1 ,P<0 .0 1,并用电针足三里后胃液酸度降低 ,p H升高 (2 .9± 0 .1) vs (1.9±0 .2 ) ,P<0 .0 1,两组胃液、血浆及胃粘膜 Gas明显增高 ,EGF含量降低 .注射西咪替丁胃液量减少 p H升高 ,胃酸度明显下降 (19.8± 0 .8) vs (2 6 .8± 2 .0 ) mmol· L- 1 ,P<0 .0 1.并用电针足三里无显著变化 .胃液、血浆、胃粘膜及颌下腺 Gas和EGF均显著升高 .结论　纳络酮促进电针足三里对胃酸分泌的抑制作用 .西咪替丁抑制胃酸分泌 ,同时促进颌下腺、胃粘膜 EGF合成与释放 .电针足三里不能改变已受抑制的胃酸分泌状态     

间断给氧对兔体外循环中肺损伤的保护

目的　研究间断给氧对兔体外循环 ( CPB)中肺的保护作用 ,并探讨其机制 .方法　采用兔 CPB模型 ,CPB中间断给氧 ,测定肺组织的丙二醛 ( MDA ) ,髓过氧化物酶( MPO) ,肺组织血管通透性 ( EB值 )及湿干质量比 ( W/ D) ,并观察肺组织超微结构的变化 .结果　间断给氧组肺组织MDA含量 ( 81± 5 ) mol.kg- 1 明显低于 CPB组 ( 139± 17) mol.kg- 1 ,P<0 .0 1.MPO含量 ( 6 .3± 1.9)× 10 3U.kg- 1 明显低于 CPB组 ( 9.4± 0 .6 )× 10 3U.kg- 1 ,P<0 .0 1.血管通透性( EB值 ) ( 1.9± 0 .2 )明显低于 CPB组 ( 3.2± 0 .0 7) ,P<0 .0 5 .W/ D值 ( 8.7± 0 .8)明显低于 CPB组 ( 11.3± 2 .3) ,P<0 .0 5 .结论　 CPB期间存在肺缺血再灌注损伤 ;间断给氧可减轻肺损伤 ,具有肺保护作用     

肝硬化患者的胃液体排空障碍

目的 :了解肝硬化与胃液体排空障碍的关系。方法 :采用实时超声胃窦面积法测定了 2 6例肝硬化患者和 14例健康人的胃液体排空 ,同时用放射免疫法检测了空腹血胃动素和胃泌素水平。结果 :肝硬化组胃液体半排空时间(GET1/ 2 )为 (34.5 3± 8.2 3) m in,较正常对照组 (2 8.0 6± 4.11) min明显延迟 (P<0 .0 1) ,表明肝硬化患者存在明显的胃液体排空障碍。肝硬化患者 GET1/ 2与上消化道症状积分呈正相关 (r=0 .42 6 ,P<0 .0 5 ) ,表明肝硬化患者的胃动力障碍是引起上消化道症状的重要原因 ;肝硬化患者 GET1/ 2与肝功能受损程度呈正相关 (r=0 .491,P<0 .0 5 ) ;肝硬化组血浆胃动素浓度为 (4 18.5 4± 5 4.6 3) ng/ L,血清胃泌素浓度为 (87.73± 2 4.37) ng/ L,均较正常对照组增高 (P均 <0 .0 1)。结论 :肝硬化患者胃液体排空障碍可能与肝功能受损、胃肠激素代谢异常有关。     

老年2型糖尿病患者胃液体排空与胃泌素、胃动素、生长抑素的关系

目的 :探讨老年 2型糖尿病患者糖尿病性胃轻瘫的发病机制。方法 :采用实时超声显像法及放射免疫法分别测定 32例老年 2型糖尿病患者胃液体排空时间和血清胃泌素、血浆胃动素及生长抑素的浓度。结果 :老年2型糖尿病患者组胃液体排空T1 2 为 (39.2 6± 10 .85 )min ,较对照组明显延迟 (P <0 .0 1)。胃液体排空延迟的 19例 2型糖尿病患者胃泌素浓度为 (14 0 .32± 30 .75 )pg·ml- 1 ,胃动素浓度为 (6 6 7.94± 14 9.32 )pg ml- 1 、生长抑素浓度为(2 8.5 4± 8.4 6 )pg·ml- 1 ,较胃液体排空正常的 13例 2型糖尿病患者分别增高、增高及下降 (P均 <0 .0 1)。老年 2型糖尿病患者胃液体排空T1 2 与胃泌素浓度呈正相关 (r =0 .94 1) ,与胃动素浓度呈正相关 (r=0 .92 5 ) ,与生长抑素浓度呈负相关 (r=- 0 .938) ,P均 <0 .0 1。结论 :老年 2型糖尿病患者存在胃运动功能障碍 ,且胃液体排空延迟与其胃泌素分泌增高、胃动素分泌增高及生长抑素分泌下降有关 ,说明胃泌素、胃动素及生长抑素在糖尿病性胃轻瘫的发病机制中起一定作用。     

应激大鼠下丘脑血管紧张素原mRNA的表达

目的　观察应激时大鼠下丘脑血管紧张素原 m RNA表达及血管紧张素 含量的变化 .方法　雄性大鼠 48只随机分为 3组 ,游泳组予以 4℃～ 6℃冷水游泳刺激 ;电击组予以0 .5～ 2 Hz,6 0～ 10 0 V足底电刺激 ,每日 2次 ,每次 2 h,持续1wk;对照组不予任何刺激 .用原位杂交技术及放免分析法检测不同应激时大鼠下丘脑血管紧张素原 (Ang) m RNA的表达及血管紧张素 (Ang )含量的变化 .结果　应激大鼠下丘脑 Ang m RNA阳性表达面积百分比与积分吸光度均显著增高 .游泳组 (2 .48± 0 .40 ) %与 (0 .75± 0 .0 9) ,电击组 (2 .94± 0 .82 ) %与 (1.83± 0 .41) vs对照组 (0 .2 0± 0 .0 4) %与 (0 .2 4± 0 .12 ) ,P<0 .0 1;Ang 含量也显著增高 .游泳组 (141±6 7) ng· g- 1 ,电击组 (84± 2 5 ) ng· g- 1 vs对照组 (5 6± 16 ) ng· g- 1 ,P<0 .0 1,P<0 .0 5 ,且 Ang m RNA表达增高率 (12 16± 196 ) %和 (144 1± 40 2 ) % ,超过了 Ang 含量的增高率(2 95± 118) %和 (149± 44 ) % ,P<0 .0 1.结论　应激时下丘脑肾素 -血管紧张素系统 (RAS)被显著激活 ,进一步支持局部RAS参与了应激反应的观点 .     

银屑病患者血中糖皮质激素及其受体mRNA的测定及其意义

目的　研究银屑病患者体内糖皮质激素水平及其受体基因表达的变化 .方法　用酶免疫法测定了 2 2例银屑病患者、15例临床治愈的银屑病患者和 2 0例正常人对照组血清糖皮质激素水平 ,用 RT- PCR方法检测 12例银屑病患者和10例正常人对照血白细胞糖皮质激素受体 m RNA的表达水平 .结果　银屑病患者组血清糖皮质激素水平 (0 .5 8± 0 .0 7μmol· L- 1 )高于银屑病临床治愈组 (0 .49± 0 .11μmol· L- 1 )和正常对照组 (0 .48± 0 .10 μmol· L- 1 ) ,差异有极显著性(P<0 .0 1) ,银屑病患者组血白细胞糖皮质激素受体 m RNA表达明显低于正常对照组 (P<0 .0 1) .结论　银屑病患者体内糖皮质激素浓度不低于正常水平 ;银屑病患者组血白细胞糖皮质激素受体 m RNA表达低于正常水平 ,提示银屑病患者体内促肾上腺皮质激素释放激素 -糖皮质激素 -糖皮质激素受体系统平衡失调 ,可能与银屑病的发病有一定关系     

艾司洛尔对心脏手术病人的血钾和乳酸的影响

目的 :观察在体外循环 (CPB)中给予艾司洛尔血钾和血乳酸的变化。方法 :选择需在体外循环下作心脏直视手术的病人 2 8例 ,观察组 (8例 )于肝素化后静脉注射初始量艾司洛尔 5 0 0 μg· kg- 1· min- 1× 4 min,继而以30 0μg· kg- 1 · m in- 1 持续输注 ,直至主动脉开放后 10 m in。对照组 (2 0例 )则在同一时期内输注相同容量的 0 .9%氯化钠。于 CPB前、CPB开始后 5 min、主动脉开放时及手术结束时作电解质和乳酸分析。结果 :观察组血钾在 CPB开始后5 m in较对照组高 (P<0 .0 1) ,两组病人血乳酸随着手术的进行渐升高 (P<0 .0 1) ;观察组血乳酸在主动脉开放时及手术结束时较对照组为低 (P<0 .0 5 )。结论 :低温体外循环引起血乳酸进行性增高 ,在 CPB中给予艾司洛尔能引起血钾升高和血乳酸的降低     

西咪替丁联合兰索拉唑治疗应激性胃溃疡的效果分析

目的探讨西咪替丁联合兰索拉唑治疗应激性胃溃疡(SGU)的疗效及安全性。方法 200例SGU患者采用随机数字表法分为对照组及观察组,各100例。对照组予西咪替丁治疗,观察组在对照组基础上予兰索拉唑治疗,比较两组治疗前后胃液p H值及胃泌素值,观察止血时间、疗效及不良反应情况。结果两组治疗前胃液p H值及胃泌素值差异均无统计学意义(均P>0.05),治疗后两组胃液p H值及胃泌素值均明显低于治疗前(均P<0.05),其中观察组低于对照组(均P<0.05)。观察组止血时间要小于对照组(P<0.05)。观察组治疗总有效率明显高于对照组(均P<0.05),两组不良反应发生率差异无统计学意义(P>0.05)。结论西咪替丁联合兰索拉唑治疗可有效改善SGU患者胃液p H值及胃泌素值,且有良好的安全性,值得临床推广。     

原因不明不孕患者子宫内膜胰岛素样生长因子-Ⅱ和Ⅰ型受体的表达与性激素的调控

目的 :探讨原因不明不孕患者子宫内膜胰岛素样生长因子 - (IGF- )及其 型受体 (IGF- R)的表达 ,血清性激素的变化与子宫内膜接受性的关系。方法 :采用逆转录聚合酶链反应 (RT- PCR)技术 ,对 34例原因不明不孕患者 (研究组 )和 2 1例正常生育妇女志愿者或男性因素不孕妇女 (对组照 )的黄体中期子宫内膜 ,行 IGF- 及 IGF- R m RNA检测 ,放射免疫法测定同期血清雌二醇 (E2 )、孕酮 (P)水平。结果 :研究组黄体中期子宫内膜IGF- m RNA、IGF- R m RNA表达水平分别为 0 .6 6 2± 0 .371、0 .5 82± 0 .2 5 7,低于对照组 0 .96 1± 0 .389、0 .82 9± 0 .341(均 P<0 .0 5 )。研究组血清 P水平为 (2 3.782± 15 .4 5 9) nmol/ L ,显著低于对照组 (43.14 2± 16 .5 49) nmol/ L(P<0 .0 0 5 ) ,而两组 E2 无显著性差异。相关分析示两组 IGF- m RNA与 IGF- R m RNA均呈正相关 ,r分别为0 .92 3、0 .738(P<0 .0 5 )。两组 P与 IGF- m RNA均呈正相关 ,r分别为 0 .716、0 .5 88(P<0 .0 5 )。两组 P与 IGF- R m RNA均呈正相关 ,r分别为 0 .5 19、0 .5 0 5 (P<0 .0 5 )。结论 :原因不明不孕患者黄体中期孕激素不足可引起子宫内膜 IGF- 及 IGF- R基因表达减弱 ,影响子宫内膜蜕膜化而导致子宫内膜接受性低下 ,     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.