母血与脐血中VitA VitC VitD VitE含量及其相关性

目的:探讨母血与脐血血清维生素正常水平值及相互关系。方法:对2002年1月～2003年6月在天坛医院妇产科进行检查的北京市崇文区540例孕产妇配对测定母血、脐血中维生素A、C、D、E浓度,比较其水平并进行相关性分析。结果:脐血与母血比较,维生素A、C、D、E差异有显著性意义,脐血维生素值普遍低于母血。母血与脐血维生素A、C、D、E具有相关性。其中尤以母血维生素A与脐血的相关性大。结论:本次研究测得母血、脐血维生素值均低于正常值。提示孕期应合理补充维生素,定期检测体内维生素的含量,母血与脐血维生素A、C、D、E含量具有相关性,妊娠期补充维生素对提高新生儿体内维生素含量有作用,以提高维生素A最容易见效。     

服用维生素E用多大剂量合适

维生素E(又名生育酚)是一种脂溶性维生素,为人体营养所必需的一种成分。从1938年被人工合成以来,广泛应用于临床,主要治疗妇产科、心血管、血液、皮肤科等多种疾病。近些年来,人们认为维生素E是一种强的抗氧化剂,具有抗衰老作用,而作为延缓衰老药物使用。     

维生素E吃还是不吃

鉴于维生素E带给人体柔肤美容的益处，一时间，兴起了“维生素E补充热”，人们只看到维生素E“光明”的一面，其实，滥用维生素E具有一定的危害。     

烟民服维生素E增加肺癌风险

美国华盛顿大学一项研究，对7．7万名常服维生素E的烟民进行了为期4年的跟踪调查发现，每日服100毫克维生素E可使烟民患肺癌的风险增加7％，每日服400毫克维生素E患肺癌风险增加28％，对吸烟量大的烟民来说，常服维生素E患肺癌的风险更大，建议烟民不要随意服用维生素E。     

维生素E的新用途

维生素E广泛存在于植物油中，其中以麦胚油和玉米油含量最多。维生素E对氧极为敏感， 易于氧化，故为抗氧化剂。维生素E在临床应用上已有70多年历史，近二三十年来人们陆续 发现了许多新的用途。1 抗癌作用近年来，国内外学者发现，维生素E对治疗宫颈癌、前列腺癌、皮肤癌及胃癌 、肺癌都具有一定的疗效。维生素E抗癌的机理在于它的强抗氧化作用。随着生物的衰老， 细胞膜上的脂质易产生过氧化反应，脂质的氧化蓄积，便容易发生癌变。而维生素E的抗氧 化作用，延缓了细胞和组织的衰老，从而延缓了癌变的发生。此外，维生素E还具有阻断致 …     

腹腔感染后抗氧化能力对血浆维生素A、E、C水平的影响

目的：观察腹腔感染后抗氧化能力对血浆维生素A、E、C水平的影响。方法：采用盲肠结扎加穿孔（CLP）模型，观察腹腔感染后丙二醛（MDA）、还原型谷胱甘肽（GSH）对血浆维生素A、E、C水平的影响。结果：CLP后血浆维生素A、C、E、GSH均明显下降，而血浆MDA显著升高，并发现血浆GSH与维生素A的变化有显著相关性，而血浆MDA与维生素C、E的变化也有显著相关性。结论：腹腔感染后由于机体抗氧化能力的下降而导致维生素A、C、E的大量消耗，可能是造成维生素A、E、C缺乏的重要原因。     

几种防癌食物

正确饮食可以有效防止癌细胞在体。内安营扎寨。蔬菜和水果含有抗氧化剂，能使游离基丧失活力，维生素C、E和胡萝卜素都有同样作用。维生素还可以相互保护，维生素C防止氧化物质破坏维生素E，维生素E能防止胡萝卜素被氧化。葡萄和红酒都有抗氧化物质，能使皮肤癌发生率降低80％。绿茶含有一种叫EGCG的物质，抑制游离基效果是维生素E的20倍，是维生素C的500倍。最近发现一些植物中的矿物质防癌作用比维生素还要强。     

055 维生素E琥珀酸酯抗肿瘤的信号传导机制

维生素E琥珀酸酯是维生素E的衍生物，具有选择性抑制肿瘤细胞生长的作用，其抗肿瘤作用机制复杂。本文综述维生素E琥珀酸酯对细胞第一信使系统(TGF-β信号传导、Fas信号传导等)、第二信使系统(Ca~(2+)、cAMP)及其它如细胞周期信号系统等的作用，为探讨维生素E琥珀酸酯抗肿瘤的信号传导机制及临床应用提供基础。     

补充维生素E可祛病延年

在美国，一位老太太从52岁起服用维生素E，活到93岁。她曾在68岁时一度停用维生素E，结果就像一般同龄妇女那样，老是觉得这里酸疼，那里又不舒服。当重新服用维生素E时，又立刻恢复了活力，而且耳聪目明，精力充沛。近期科学研究表明。维生素E对人体具有如下重要保健功能：     

维生素E联合维生素C对染石英尘肺泡巨噬细胞脂质过氧化和抗氧化酶的影响

目的 探讨维生素E与维生素C联合抗石英尘细胞毒作用及其机制。方法 采用体外细胞培养法，研究不同浓度维生素E与维生素C对染色英尘的肺泡巨噬细胞（AM）膜脂质过氧化和抗氧化酶的影响。结果 在染石英尘的AM中加入维生素E与维生素C，可以减少活性氧，丙二醛的生成，与石英组比较，差异有显著性；而过氧化氢酶，谷胱甘肽过氧化物酶，超氧化物歧化酶的活力和细胞活力高于石英组。结论 维生素E联合维生素C对染石英尘的肺泡巨噬细胞具有保护作用。     

HPV16E6、E7蛋白在肺癌组织中的表达及意义

目的研究漯河周边地区非小细胞肺癌(non small cell lung cancer,NSCLC)病变组织中HPV16E6、E7蛋白表达情况及与临床病理参数之间的关系,探讨HPV16E6和E7蛋白在NSCLC发病机制中的作用。方法 2010年3月—2011年6月采用免疫组织化学技术分别检测80例NSCLC标本和55例癌周对照组织中HPV16E6和E7蛋白的表达,并进行对比,计数资料采用χ2检验或确切概率法,P0.05为差异有统计学意义。结果 80例NSCLC组织中,HPV16 E6、E7、E6/E7蛋白的表达率分别为31.3%、33.8%和28.8%,55例癌旁组织中,HPV16E6、E7、E6/E7蛋白表达阳性率分别为14.5%、9.2%、7.3%。NSCLC组织中E6、E7和E6/E7蛋白共表达阳性率均高于对照组,差异有统计学意义(P0.05),与患者年龄、性别以及肿瘤组织学类型无关,但与肿瘤分化以及淋巴结转移有关。结论 HPV16E6和E7蛋白在NSCLC发生发展中具有重要作用。     

HPV E6/E7研究现状及临床展望

人乳头状瘤病毒是宫颈癌最重要的致病因素,其早期蛋白E6/E7的持续表达可致细胞p53、pRb蛋白缺失或失活,并通过多种机制与其他细胞因子相互作用,促使细胞发生癌变。其mRNA表达水平提示病毒转录活性,可能具有诊断和预测宫颈病变的价值,因此有研究应用HPV E6/E7 mRNA筛查不同人群宫颈病变并随访、预测病变发生风险,但各研究结果不一,不同mRNA检测方法也是影响研究结果的因素之一。HPV E6/E7蛋白检测在临床应用较少,而以HPV E6/E7为靶点的宫颈癌免疫治疗已成为研究的热点,在动物实验及临床前研究中取得了一定进展。     

Immunogenicity of the E1E2 proteins of hepatitis C virus expressed by recombinant adenoviruses

The E1 and E2 proteins of hepatitis C virus (HCV) are believed to be the viral envelope glycoproteins that are major candidate antigens for HCV vaccine development. We reported previously that the replication-competent recombinant adenovirus encoding core-E1-E2 genes of HCV (Ad/HCV) produces serologically reactive E1 and E2 proteins forming a heterodimer in substantial amounts. Here, we examined immunogenicity of the E1E2 proteins copurified from HeLa cells infected with Ad/HCV virus in mice. Furthermore, we constructed a replication-defective recombinant adenovirus encoding the core-E1-E2 genes of HCV (Ad.CMV.HCV) and examined immunogenicity of the virus in mice. The mice immunized intraperitoneally with the copurified E1E2 proteins induced mainly antibodies to E2, but not to E1 by Western blot analysis. The sera of mice immunized with the E1E2 inhibited the binding of E2 protein to the major extracellular loop of human CD81. E2-specific cytotoxic T cells (CTLs), but not antibodies to the E1E2 antigens were induced in the mice intramuscularly immunized with Ad.CMV.HCV virus. When immunized with both Ad.CMV.HCV virus and the E1E2, mice elicited E2-specific CTLs and antibodies to the E1E2 antigens. The results suggest that immunization of Ad.CMV.HCV virus combined with E2 protein is an effective modality to induce humoral as well as cellular immune response to E2 antigen.     

人乳腺癌细胞增殖实验检测典型雌激素复合效应

目的 研究4种典型雌激素17β雌二醇(E2)、乙炔雌二醇(EE2)、雌三醇(E3)、雌酮(E1)复合作用对人乳腺癌细胞(MCF-7)乳腺癌增殖效应的影响,并比较独立作用模型(IA模型)和浓度加和模型(CA模型)对雌激素复合效应评价的优劣.方法 选用E2+EE2、E2+E3和E2+E1两类雌激素物质的联合组合设计,噻唑蓝(MTT)法检测MCF-7细胞增殖效应.结果 E2在10-9mol/L最大增殖效应为325.48%,EE2在10-9 mol/L最大增殖效应为330.34%,E3在10-9 mol/L最大增殖效应为255.22%,E1在10-9 mol/L最大增殖效应为199.61%;E2+EE2、E2+E3、E2+E1 3组摩尔浓度配比下IA模型拟合结果与实验结果十分接近,IA模型往往高估实验结果,而CA模型往往低估实验结果;除EC(E2,30)+C(EE2,70)这组结果超过E2所表现出的最大雌激素效应(即效应比小于1)外,其中各组结果均弱于E2表现出的最大增殖效应.结论 4种受试物雌激素效应依次为EE2>E2>E3>E1;IA模型对二元混合物复合效应的预测结果优于CA模型;二元混合物表现出的协同作用比例较小.     

Vitamin E improves microsomal phospholipase A2 activity and the arachidonic acid cascade in kidney of diabetic rats

The purpose of the present study was to investigate the effects of vitamin E on microsomal phospholipase A2 activity and the arachidonic acid cascade in the kidneys of streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 100 +/- 10 g were randomly assigned to one normal and three STZ-induced diabetic groups. The diabetic groups were fed a vitamin E-free diet (the DM-0E group), 40 mg vitamin E/kg diet (the DM-40E group) or a 400 mg vitamin E/kg diet (the DM-400E group). The kidney vitamin E concentrations were 59 and 49% lower in the DM-0E and DM-40E groups, respectively, than in the normal group. The kidney thiobarbituric acid reactive substance concentrations in the DM-0E, DM-40E and DM-400E groups were 119, 84 and 33% greater, respectively, than that in the normal group. The concentration in the DM-400E group was 39% lower than that in the DM-0E group. The phospholipase A2 (PLA2) activity in the kidney microsomes of the DM-0E-40E and DM-400E groups were 88, 58 and 35% greater, respectively, than that in the normal group. The activity in the DM-400E group was 28% lower than that in the DM-0E group and 16% lower than that in the DM-40E group. The differences in the phospholipids in the kidney microsomes included reductions in the phosphatidylcholine and phosphatidylethanolamine compositions. Phosphatidylethanolamine hydrolysis in the kidney microsomes of the DM-0E and DM-40E groups were 84 and 64%, which did not differ from the DM-400E group. The formation of thromboxane A2 (TXA2) in the kidney microsomes was 137 and 70% greater in the DM-0E and DM-40E groups, respectively, than in the normal group. TXA2 formation did not differ between the DM-400E and normal groups. The formation of prostacyclin in the kidney microsomes was 60 and 44% lower in the DM-0E and DM-40E groups, respectively, than in the normal group, whereas the DM-400E group did not differ from that in the normal group. The ratio of prostacyclin to TXA2 was 82 and 65% lower than normal in the DM-0E and DM-40E groups, respectively. Kidney function appears to be improved by vitamin E supplementation due to its antithrombus action, which in turn controls the arachidonic acid cascade system.     

APOE polymorphism and the hypertriglyceridemic effect of dietary sucrose

BACKGROUND: The E4 allele of the apolipoprotein gene (APOE) is associated with a greater serum cholesterol response to dietary changes in fat and cholesterol. However, less is known about the interaction between APOE polymorphism and other macronutrients in the diet. OBJECTIVE: We evaluated the interaction between APOE polymorphism and dietary fat and carbohydrate, particularly sucrose, in relation to serum lipid concentrations. DESIGN: A total of 284 men and 130 women with coronary artery disease (mean age: 61 y; range: 33-74 y) participated in the cross-sectional EUROASPIRE study. Serum lipids and fatty acids in cholesteryl esters (CEs) were measured and APOE genotypes were determined. Dietary intake was examined by using a 4-d food record. RESULTS: Patients were grouped by APOE genotype: E2 (E2/E2 and E2/E3; n = 21), E3 (E3/E3; n = 245), and E4 (E4/E2, E4/E3, and E4/E4; n = 148). Patients with the E2 allele had lower LDL-cholesterol concentrations and tended to have higher triacylglycerol concentrations than did patients with the E3 or E4 allele; concentrations were not significantly different between the last 2 groups. In regression analysis, significant predictors of serum triacylglycerol were the interaction between sucrose intake and the E2 allele, proportion of n-3 fatty acids in CEs, body mass index, and diabetes. A high sucrose intake was associated with high triacylglycerol concentrations only in patients with the E2 allele. Interaction between saturated fat intake and the E2 allele, proportion of linoleic acid in CEs, and fiber intake predicted serum cholesterol. CONCLUSION: Coronary artery disease patients with the E2 allele will likely have a greater triacylglycerol response to high dietary sucrose intakes than will patients with the E3 or E4 allele.     

Normal vitamin E status in sickle hemoglobinopathies in Colorado

Because previous studies of serum or plasma vitamin E (E) levels reported a high prevalence of E deficiency in patients with sickle cell anemia (SCA), we studied the E status in 101 patients with SCA in Colorado using both levels of serum E and ratios of serum E to total lipid (E:L). Compared with age-, sex-, and race-matched controls, 1 of 70 patients with homozygous SCA (SS), 1 of 7 with sickle beta+-thalassemia, and 0 of 24 with hemoglobin SC disease had E deficiency according to E:L and all were E-sufficient based on serum E levels. Serum cholesterol levels, lower in SS patients than in control subjects, correlated more strongly with serum E levels than did total serum lipid levels in control subjects and SS patients; hence, the ratio of serum E to cholesterol may be a useful indicator of E status in these patients. We conclude that vitamin E deficiency rarely occurs in SCA patients in Colorado.     

人乳头状瘤病毒E6/E7 mRNA检测宫颈高度病变的临床评价

目的 评价人乳头状瘤病毒(HPV)E6/E7 mRNA检测宫颈高度病变的效能.方法 用PreTect~(TM) HPV-Proofer检测法检测65例妇女宫颈上皮细胞HPV E6/E7 mRNA转录情况,以液基细胞学检测(LBC)结果为标准评价HPV E6/E7 mRNA检测宫颈高度病变[不能除外高度不典型鳞状细胞(≥ASC-H)]的效能.结果 高度组(≥ASC-H组)HPV E6/E7 mRNA转录阳性率(63.6%)高于低度组(相似文献   

Entamoeba moshkovskii infections in children,Bangladesh

Entamoeba moshkovskii cysts are morphologically indistinguishable from those of the disease-causing species E. histolytica and the nonpathogenic E. dispar. Although sporadic cases of human infection with E. moshkovskii have been reported, the organism is considered primarily a free-living amoeba. No simple molecular detection tool is available for diagnosing E. moshkovskii infections. We used polymerase chain reaction (PCR) to detect E. moshkovskii directly in stool. We tested 109 stool specimens from preschool children in Bangladesh by PCR; 17 were positive for E. histolytica (15.6%) and 39 were positive for E. dispar (35.8%). In addition, we found that 23 (21.1%) were positive for E. moshkovskii infection, and 17 (73.9%) of these also carried E. histolytica or E. dispar. The high association of E. moshkovskii with E. histolytica and E. dispar may have obscured its identification in previous studies. The high prevalence found in this study suggests that humans may be a true host for this amoeba.     

Intramuscular injection of plasmid DNA encoding cottontail rabbit papillomavirus E1, E2, E6 and E7 induces T cell-mediated but not humoral immune responses in rabbits

To test the efficacy of genetic vaccination against papillomavirus infection, plasmid DNA encoding cottontail rabbit papillomavirus (CRPV) E1, E2, E6, E7 or without insert were intramuscularly injected into five groups of rabbits. Peripheral blood mononuclear cells (PBMCs) showed specific proliferation upon in vitro stimulation with E1, E2, E6 or E7 proteins in a majority of vaccinated rabbits but Western blot analysis did not detect antibodies specific for these viral proteins in rabbit serum. All rabbits grew papillomas after virus challenge and none of the rabbits showed systemic papilloma regression. These observations showed that intramuscular injection of plasmid DNA encoding CRPV E1, E2, E6 or E7 induced CD4+ T cell-mediated but not humoral immune responses, and did not result in the protection of rabbits from virus infection.