针刺对快速老化脑萎缩模型小鼠脑抗氧化酶活性的影响

目的：观察针刺“水沟”,“内关”,“太冲”穴对快速老化脑萎缩模型小鼠（SAM－P／10）脑丙二醛含量（MDA）,超氧化物歧化酶（SOD）,过氧化氢酶（CAT）,谷胱甘肽过氧化物酶（GSH－Px)活性的影响,结果：针刺可降低模型动物MDA含量,提高抗氧化酶活性,结论：抗氧化酶活性降低可能是脑老化的重要因素,针刺可从整个抗氧化体系水平提高机体抗氧化能力,减少自由基代谢产物,从而起到延缓脑老化的作用。     

显齿蛇葡萄叶提取物对苯并芘染毒小鼠血清和肝脏抗氧化酶的影响

目的：探讨显齿蛇葡萄叶提取物对苯并芘染毒小鼠血清和肝脏抗氧化酶的影响。方法：用显齿蛇葡萄叶提取物给小鼠灌服2周后,一次性灌胃苯并芘染毒,取血和肝脏测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）、过氧化氢酶（CAT）、γ-谷氨酰转肽酶（γ-GT）活性和丙二醛（MDA）含量。结果：显齿蛇葡萄叶提取物能明显提高小鼠血清和肝脏中SOD、GSH—PX、CAT的活性,降低γ-GT活性及MDA含量。结论：显齿蛇葡萄叶提取物具有抗氧化作用,可能是其抗致癌物作用机制之一。     

四君子汤对老年脾气虚证患者抗氧化能力的影响

目的探讨四君子汤对老年脾气虚证患者自由基代谢失衡的调节作用及其延缓衰老的机制。方法治疗组42例运用四君子汤治疗,观察治疗前后血浆Mn-超氧化物歧化酶（Mn—SOD）、血浆丙二醛（MDA）的水平、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH—Px）活性及总抗氧化活性,并与正常对照组41例进行比较。结果老年脾气虚证患者治疗前血浆Mn—SOD水平、GSH—Px活性和总抗氧化活性均较对照组明显降低（P〈0．01）,血浆MDA水平明显升高（P〈0．01）,经四君子汤治疗后,上述指标均恢复正常。结论老年脾气虚证患者自由基清除能力下降。四君子汤能提高患者的抗氧化能力,消除或减少自由基对机体的损害。     

中药复方柴胡疏肝散体内外抗氧化活性及其与抗抑郁作用的 潜在关系

目的：研究中药复方柴胡疏肝散（CSGS）的体内外抗氧化活性, 探讨其抗氧化活性与抗抑郁作用之间的联系, 助以阐释CGGS抗抑郁的作用机制。方法：采用总抗氧化能力测定（FRAP法）, DPPH （1 ,1-二苯基-2-苦基苯肼） 自由基（DPPH法）、ABTS[ 2 ,2-连氮（3-乙基苯并噻唑啉-6-磺酸）二铵盐]阳离子自由基（ABTS法）、超氧阴离子（O2·^-）及羟自由基（·OH）清除能力测定5 种方法, 以维生素C为阳性对照, 研究CSGS体外抗氧化活性。建立束缚氧化应激模型, 通过测定全血中谷胱甘肽（GSH）含量, 肝组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MAD）含量研究CSGS体内抗氧化作用。结果：CSGS体外具有一定的铁离子还原能力和自由基清除能力, 其还原能力（FRAP法）每克CSGS提取物相当于 0.24 mmol FeSO4, 清除DPPH自由基、ABTS阳离子自由基、O2·^-及 ·OH的IC50分别为 0.83, 1.03, 10.31 和 7.79 mg·mL^-1。体内抗氧化活性研究显示, 在束缚应激状态下, CSGS给药组小鼠与模型组小鼠比较具有相对高的SOD和CAT活性及GSH含量, 相对较低的MDA水平。结论：复方柴胡疏肝散具有抑制脂质过氧化、缓解氧化应激损伤、调控机体抗氧化水平的药理作用, 其抗抑郁作用可能与其抗氧化活性有一定相关。     

脾、肾阳虚模型大鼠脂质过氧化与抗氧化相关性的比较研究

目的：探讨实验性脾、肾阳虚模型大鼠脂质过氧化与抗氧化的相关性。方法：采用经典方法分别复制脾阳虚证和肾阳虚证动物模型。观察并对比了两种模型大鼠和相应治疗组的脂质过氧化物(LPo)和谷胱甘肽过氧化物酶(GSH—Px)以及GSH—Px／LPO(抗氧化能力的重要参数)的变化。结果：脾、肾阳虚模型大鼠LPO含量增加,GSH—Px活性及GSH—Px／LP0比值下降,且组间差异显著。温补脾肾药物具有降低LPO含量,提高GSH—Px活性及抗氧化能力的作用。结论：脂质过氧化损伤是脾、肾阳虚证的共同病理生理基础。     

锁阳提取物对衰老小鼠抗氧化作用的比较

目的：研究锁阳的不同提取液对D-半乳糖致衰小鼠机体的影响。方法：建立小鼠衰老模型,以锁阳的不同提取液给药8周,取血并测定其过氧化氢酶（CAT）、总抗氧化能力、谷胱甘肽过氧化物酶（GSH—PX）三项指标。结果：不同的提取物能明显降低小鼠机体的氧化程度。尤其锁阳醇提物的三项指标,与空白对照组相比无显著性差异,而与D-半乳糖致衰小鼠相比则具有显著性差异。结论：锁阳不同提取物均具有较明显的抗氧化及抗衰老能力,其中醇提物作用最显著。     

广东凉茶颗粒对应激小鼠生殖器官过氧化状态的改善作用

目的研究广东凉茶颗粒对拘束负荷诱发小鼠生殖器官过氧化状态的改善作用。方法18h拘束负荷诱发小鼠生殖器官过氧化损伤，分别利用硫代巴比妥酸法测定睾丸和卵巢丙二醛（MDA）水平，HPLC法测定谷胱甘肽（GSH）含量，比色法测定谷胱甘肽过氧化物酶（GSH—Px）和黄嘌呤氧化酶（XOD）活性，Griess化学法测定一氧化氮（NO）含量，荧光酶标仪测定抗氧化能力指数（ORAC）。结果与拘束负荷模型组相比，广东凉茶颗粒可以明显降低因拘束负荷引起的小鼠睾丸和卵巢组织MDA水平、NO含量和XOD活性升高，同时显著升高睾丸和卵巢组织中的GSH含量、GSH—Px活性和ORAC指数。结论广东凉茶颗粒对拘束负荷诱发的小鼠生殖器官过氧化状态具有一定的改善作用，其作用可能与减少拘束负荷小鼠的氧化应激水平和改善组织脂质过氧化过程有关。     

荷S180小鼠血清MDA、SOD和GSH-PX的变化及无花果多糖对其影响

目的：观察无花果多糖对荷瘤小鼠血清丙二醛，超氧化物歧化酶和谷胱甘肽过氧化物酶的影响。方法：以荷S180实体瘤小鼠为模型，灌胃给药，连续10d，测定小鼠血清中SOD，GSH－PX的活性及MDA的含量。结果：无花果多糖可提高荷瘤小鼠血中抗氧化酶的活性，降低脂质过氧化物的含量。结论：无花果多糖的抗瘤作用可能与提高SOD，GSH－PX活性，和降低自由基水平有关。     

甘草附子汤对AA大鼠氧自由基代谢影响的配伍规律研究

目的：探讨甘草附子汤对佐剂性关节炎大鼠抗炎及抗氧化的作用。方法：观察甘草附子汤及其不同配伍对佐剂性关节炎大鼠关节炎的预防治疗作用，以及对红细胞超氧化物歧化酶（SOD）、全血谷胱甘肽过氧化物酶（GSH-PX）活性、血浆丙二醛（MDA）、血清一氧化氮（NO）含量的影响。结果：全方及各配伍组均能显著抑制关节炎大鼠的关节炎，使佐剂性关节炎（AA）大鼠过高的MDA、NO降低，使已降低的SOD、GSH—PX升高。结论：甘草附子汤对大鼠佐剂性关节炎有治疗作用，此作用可能与其降低制脂质过氧化，恢复抗氧化酶活性，抑制致炎因子NO的合成等有关；且在抗氧化方面全方组疗效明显优于任何一组配伍。     

首乌延寿丹抗血管内皮细胞老化的实验研究

目的：探讨首乌延寿丹对动脉粥样硬化中血管内皮细胞（EC）损伤的抑制作用，方法：以高脂血清作用于培养的人脐带静脉内皮细胞，诱发内皮细胞脂质过氧化损伤，同时加入中药首乌延寿丹提取液作为保护剂，应用脂质过氧化物含量及酶活性测定以及放射免疫分析等方法，观察中药对脂质过氧化损伤的内皮细胞结构和功能的保护作用。结果：首乌延寿丹能有效增加机体抗氧化能力，抑制脂质过氧化反应，减少细胞内脂质过氧化物（LPO）的蓄积，提高谷胱甘肽过氧化物酶（GSH－PX）活性，抑制前列环素（PGI2）合成减少，保护内皮细胞，减轻细胞及细胞膜的结构的损伤程度，从而达到抗动脉粥样硬化（AS），抗衰老的功效。     

Antioxidant and free-radical scavenging activities of Allium roseum and Allium subhirsutum

This study was designed to examine the antioxidant and free-radical scavenging activities of two Allium species, Allium roseum and Allium subhirsutum. This study reports the results concerning bulb, stalk and leaf antioxidant enzyme activities (superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase), quantities of malonyldialdehyde, (*)OH and O(2) (*-) radicals and total antioxidant capacity determined by the FRAP method. Scavenging activities were determined by ESR method. The total antioxidant capacity was the highest in the leaves of Allium roseum. Among the investigated organs the leaves exhibited the highest antioxidant activities in all investigated Allium species. The highest ESR (84.61%) scavenger activity was observed in the leaves of Allium roseum.     

Scavenger activity of Allium psekemense B. Fedtsch

In the present study the antioxidative properties of the bulbs, leaves and stalks of Allium psekemense B. Fedtsch were investigated. The activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, glutathione peroxidase), quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and also the content of total flavonoids, chlorophylls a and b, carotenoids, vitamin C and soluble proteins were determined. The results indicate that extracts from all plant organs exhibited antioxidant activity. The highest antioxidant activity was observed in the leaves. Furthermore, the ESR signal of DMPO-OH radical adducts in the presence of the leaf phosphate buffer (pH 7) extract was reduced by 54.3%.     

Role of mangiferin on biochemical alterations and antioxidant status in isoproterenol-induced myocardial infarction in rats

The current study dealt with the protective role of mangiferin, a polyphenol from Mangifera indica Linn. (Anacardiaceae), on isoproterenol (ISPH)-induced myocardial infarction (MI) in rats through its antioxidative mechanism. Subcutaneous injection of ISPH (200 mg/kg body weight in 1 ml saline) to rats for 2 consecutive days caused myocardial damage in rat heart, which was determined by the increased activity of serum lactate dehydrogenase (LDH) and creatine phosphokinase isoenzymes (CK-MB), increased uric acid level and reduced plasma iron binding capacity. The protective role of mangiferin was analyzed by triphenyl tetrazolium chloride (TTC) test used for macroscopic enzyme mapping assay of the ischemic myocardium. The heart tissue antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, glutathione transferase and glutathione reductase activities, non-enzymic antioxidants such as cerruloplasmin, Vitamin C, Vitamin E and glutathione levels were altered in MI rats. Upon pretreatment with mangiferin (100 mg/kg body weight suspended in 2 ml of dimethyl sulphoxide) given intraperitoneally for 28 days to MI rats protected the above-mentioned parameters to fall from the normal levels. Activities of heart tissue enzymic antioxidants and serum non-enzymic antioxidants levels rose significantly upon mangiferin administration as compared to ISPH-induced MI rats. From the present study it is concluded that mangiferin exerts a beneficial effect against ISPH-induced MI due to its antioxidant potential, which regulated the tissues defense system against cardiac damage.     

Comparative study on Allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant status

This study was designed to examine Allium schoenoprasum tissue culture organs antioxidant and scavenging activity and to make a comparison between Allium schoenoprasum cultivated plant and Allium schoenoprasum tissue culture organs antioxidant activity. This study reports the results on the root, stalk and leaf antioxidant enzyme activities (superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase), reduced glutathione quantity, flavonoids and soluble protein contents and quantities of malonyldialdehyde and ·OH radical. In Allium schoenoprasum tissue culture organs the total antioxidant capacity was determined by the FRAP method and scavenger activity by the DPPH method. The present results indicated that the crude extract of Allium schoenoprasum tissue culture exhibited antioxidant and scavenging abilities in all investigated plant parts, especially in the roots. According to our results, the tissue culture plants exhibited the highest activities in the roots in contrast to the cultivated plants where highest activities were observed in the leaves.     

Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice

Oxidative damage of biomolecules increases with age and is postulated to be a major causal factor of various physiological function disorders. Consequently, the concept of anti-age by antioxidants has been developed. Lycium barbarum fruits have been used as a traditional Chinese herbal medicine and the data obtained in in vitro models have clearly established the antioxidant potency of the polysaccharides isolated from the fruits. In the present study, the age-dependent changes in the antioxidant enzyme activity, immune function and lipid peroxidation product were investigated and effect of Lycium barbarum polysaccharides on age-induced oxidative stress in different organs of aged mice was checked. Lycium barbarum polysaccharides (200, 350 and 500 mg/kg b.w. in physiological saline) were orally administrated to aged mice over a period of 30 days. Aged mice receiving vitamin C served as positive control. Enzymatic and non-enzymatic antioxidants, lipid peroxides in serum and tested organs, and immune function were measured. Result showed that increased endogenous lipid peroxidation, and decreased antioxidant activities, as assessed by superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC), and immune function were observed in aged mice and restored to normal levels in the polysaccharides-treated groups. Antioxidant activities of Lycium barbarum polysaccharides can be compable with normal antioxidant, vitamin C. Moreover, addition of vitamin C to the polysaccharides further increased the in vivo antioxidant activity of the latter. It is concluded that the Lycium barbarum polysaccharides can be used in compensating the decline in TAOC, immune function and the activities of antioxidant enzymes and thereby reduces the risks of lipid peroxidation accelerated by age-induced free radical.     

Evaluation of antidiabetic, antioxidant and vasoprotective effects of Posidonia oceanica extract

The aim of this study is to evaluate antidiabetic, antioxidant and vasoprotective effects of Posidonia oceanica extract (POE) in alloxan diabetic rats. Posidonia oceanica (L) Delile (Posidoniaceae), is a widely allocated phanerogam in Mediterranean and Aegean Sea. Up to date, no published data relevant to use of the plant in traditional medicine are available. However, decoction of the leaves has been quoted to be used as a remedy for diabetes mellitus and hypertension by villagers living by the sea coast of Western Anatolia. Oral administration of extract for 15 days (50, 150, and 250 mg/kg b.wt.) resulted in a dose-dependent decrease in blood glucose. Relaxant responses to acetylcholine (ACh) in diabetic thoracic aorta were restored by POE treatment (50, 150, and 250 mg/kg b.wt.). POE also attenuated the augmented phenylephrine (PE) and serotonin (5-HT) contractions. At concentration levels of 150 and 250 mg/kg b.wt., POE exerted a protective effect on the significantly decreased levels of antioxidants namely, glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and nitric oxide (NO). POE (50mg/kg b.wt.) produced no effect on alloxan-induced alterations in the antioxidant status while possessing glucose lowering and vasoprotective activities. Furthermore, liver and kidney function markers, leucocyte counts, body weight and liver glycogen content remained unchanged at dose level of 50mg/kg b.wt., when compared with diabetic control group. These results suggest that antidiabetic and vasoprotective effects of POE may be unrelated to its antioxidant properties.     

Evaluation of willow herb's (Epilobium angustofolium L.) antioxidant and radical scavenging capacities

The present study investigated the antioxidative properties of root, leaf and stalk of Epilobium angustofolium L. The activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase), quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and also the content of total flavonoids, chlorophylls a and b, carotenoids, vitamin C and soluble proteins were determined. The results indicate that extracts from all plant organs exhibited antioxidant activity. The highest antioxidant activity was observed in the leaves. Radical scavenging capacity (RSC), determined by DPPH assay, was highest in the leaves as well as total reducing power determined by FRAP assay. Lipofuscin 'plant age pigments' were also determined.     

竹叶水溶性提取液抗氧化作用的实验研究

目的：探计竹叶的水溶性提取液对动物的抗氧化作用。方法：分别灌胃给予自然衰老小鼠和D-半乳糖致衰老大鼠竹叶提取液，检测动物全血的超氧化歧化酶(SOD)和谷胱甘肽过氧物酶(GSH—Px)活性，并检测心、脑组织中脂褐质(LF)、丙二醛(MDA)和SOD含量。结果：给予竹叶提取液的衰老小鼠和大鼠心、脑组织中SOD和全血中SOD和GSH—Px活力显著提高，而组织中MDA和LF含量则明显下降。结论：竹叶提取液能提高组织中抗氧化酶的活性，抑制自由基损伤，防止脂质过氧化，具有明显的抗氧化作用。     

Exploring Equisetum arvense L., Equisetum ramosissimum L. and Equisetum telmateia L. as sources of natural antioxidants

The antioxidant and scavenging activities of above ground parts of Equisetum arvense L., Equisetum ramosissimum L. and Equisetum telmateia L. phosphate buffer (pH 7) extracts were investigated. Activities of antioxidant enzymes (superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase), quantities of reduced glutathione, malonyldialdehyde, superoxide and hydroxyl radicals and flavonoid, soluble protein, chlorophyll a, b and carotenoid contents were determined. The total antioxidant capacity was determined by ferric reducing antioxidant power (FRAP) assay. The Equisetum telmateia extract demonstrated scavenging and antioxidant properties better than Equisetum ramosissimum and Equisetum arvense. The ESR signal of DMPO‐OH radical adducts in the presence of Equisetum telmateia phosphate buffer (pH 7) extract was reduced by 98.9% indicating that Equisetum telmateia could be a useful source of antioxidants with huge scavenging ability. Copyright © 2008 John Wiley & Sons, Ltd.     

An investigation into the antioxidant activity of Allium nutans L.

Antioxidants are important species which possess the ability to protect the body from damage caused by free radical‐induced oxidative stress. There is currently much interest in the antioxidant role of fruit, vegetables, wines and teas. In this study the antioxidant activity of leaf, bulb and root of Allium nutants L. was investigated. Biochemical parameters were also determined: activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, glutathione peroxidase), quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and contents of total flavonoids, chlorophylls a and b carotenoids, vitamin C and soluble proteins. Our results indicated that Allium nutants L. exhibits antioxidant ability in all investigated plant organs. The highest antioxidant ability was observed in the leaves where all investigated antioxidant enzymes were active and quantities of malonyldialdehyde and OH· low. Reduced glutathione, pigments and carotenoids present in the leaves contribute to the high antioxidant activity. ESR investigation conducted with Allium nutans L. phosphate buffer (pH 7) extract showed that the signal DMPO‐OH spin adducts in the presence of Allium nutans L. extract was reduced by 78.48%. Copyright © 1999 John Wiley & Sons, Ltd.