Pygmy locomotion

The hypothesis that Pygmies may differ from Caucasians in some aspects of the mechanics of locomotion was tested. A total of 13 Pygmies and 7 Caucasians were asked to walk and run on a treadmill at 4–12 km · h^–1. Simultaneous metabolic measurements and three-dimensional motion analysis were performed allowing the energy expenditure and the mechanical external and internal work to be calculated. In Pygmies the metabolic energy cost was higher during walking at all speeds (P < 0.05), but tended to be lower during running (NS). The stride frequency and the internal mechanical work were higher for Pygmies at all walking (P < 0.05) and running (NS) speeds although the external mechanical work was similar. The total mechanical work for Pygmies was higher during walking (P < 0.05), but not during running and the efficiency of locomotion was similar in all subjects and speeds. The higher cost of walking in Pygmies is consistent with the allometric prediction for smaller subjects. The major determinants of the higher cost of walking was the difference in stride frequency (+9.45, SD 0.44% for Pygmies), which affected the mechanical internal work. This explains the observed higher total mechanical work of walking in Pygmies, even when the external component was the same. Most of the differences between Pygmies and Caucasians, observed during walking, tended to disappear when the speed was normalized as the Fronde number. However, this was not the case for running. Thus, whereas the tested hypothesis must be rejected for walking, the data from running, do indeed suggest that Pygmies may differ in some aspects of the mechanics of locomotion.     

Activity of pyramidal tract neurons in the cat during standing and walking on an inclined plane

To keep balance when standing or walking on a surface inclined in the roll plane, the cat modifies its body configuration so that the functional length of its right and left limbs becomes different. The aim of the present study was to assess the motor cortex participation in the generation of this left/right asymmetry. We recorded the activity of fore- and hindlimb-related pyramidal tract neurons (PTNs) during standing and walking on a treadmill. A difference in PTN activity at two tilted positions of the treadmill (± 15 deg) was considered a positional response to surface inclination. During standing, 47% of PTNs exhibited a positional response, increasing their activity with either the contra-tilt (20%) or the ipsi-tilt (27%). During walking, PTNs were modulated in the rhythm of stepping, and tilts of the supporting surface evoked positional responses in the form of changes to the magnitude of modulation in 58% of PTNs. The contra-tilt increased activity in 28% of PTNs, and ipsi-tilt increased activity in 30% of PTNs. We suggest that PTNs with positional responses contribute to the modifications of limb configuration that are necessary for adaptation to the inclined surface. By comparing the responses to tilts in individual PTNs during standing and walking, four groups of PTNs were revealed: responding in both tasks (30%); responding only during standing (16%); responding only during walking (30%); responding in none of the tasks (24%). This diversity suggests that common and separate cortical mechanisms are used for postural adaptation to tilts during standing and walking.     

Modulation of stretch reflexes during imposed walking movements of the human ankle.

Our overall objectives were to examine the role of peripheral afferents from the ankle in modulating stretch reflexes during imposed walking movements and to assess the mechanical consequences of this reflex activity. Specifically we sought to define the changes in the electromyographic (EMG) and mechanical responses to a stretch as a function of the phase of the step cycle. We recorded the ankle position of a normal subject walking on a treadmill at 3 km/h and used a hydraulic actuator to impose the same movements on supine subjects generating a constant level of ankle torque. Small pulse displacements, superimposed on the simulated walking movement, evoked stretch reflexes at different phases of the cycle. Three major findings resulted: 1) soleus reflex EMG responses were influenced strongly by imposed walking movements. The response amplitude was substantially smaller than that observed during steady-state conditions and was modulated throughout the step cycle. This modulation was qualitatively similar to that observed during active walking. Because central factors were held constant during the imposed walking experiments, we conclude that peripheral mechanisms were capable of both reducing the amplitude of the reflex EMG and producing its modulation throughout the movement. 2) Pulse disturbances applied from early to midstance of the imposed walking cycle generated large reflex torques, suggesting that the stretch reflex could help to resist unexpected perturbations during this phase of walking. In contrast, pulses applied during late stance and swing phase generated little reflex torque. 3) Reflex EMG and reflex torque were modulated differently throughout the imposed walking cycle. In fact, at the time when the reflex EMG response was largest, the corresponding reflex torque was negligible. Thus movement not only changes the reflex EMG but greatly modifies the mechanical output that results.     

Comparison of inversion restraint provided by ankle prophylactic devices before and after exercise

The prudence of prophylactic ankle taping continues to be questioned as recent studies have identified other forms of ankle stabilization as more effective means of injury prevention. The purpose of our study was to compare the effectiveness of three ankle prophylaxes (adhesive taping, lace-up brace, and semirigid orthosis) with a control condition (no support) in limiting inversion under dynamic loads imposed by repetitive walking (4 mph) and running (9 mph) on an 8.5° laterally tilted treadmill. Ten subjects participated in four separate testing sessions in which they were videotaped while walking and running on a tilted treadmill before and after 20 minutes of vigorous exercise. Average maximum inversion angle was determined through biomechanical analysis of rearfoot motion for each experimental condition and analyzed with repeated measures ANOVA and Scheffé post hoc tests. There were significant differences in the average maximum inversion angle between the ankle devices at 4 and 9 mph, and between pre-exercise and postexercise measurements at 4 mph, between the semirigid orthosis and the control condition at 4 and 9 mph, and between the lace-up brace and the control condition at 4 mph. Overall, the semirigid orthosis provided the most inversion restraint during dynamic loading, followed by the lace-up brace, tape, and control condition. We concluded that the lace-up brace and semirigid orthosis evaluated were very similar in restricting inversion, and that both devices limited postexercise inversion significantly more than ankle taping.     

Central set influences on gait. Age-dependent effects of postural threat

We tested the hypothesis that anxiety regarding the potential consequences of a possible fall would alter gait patterns differently between younger and older adults. Sixteen younger and fifteen older adults participated in this study. Participants walked at a self-determined velocity along a 7.2-m walkway under 4 different conditions of postural threat; the walking conditions varied depending on the width constraints of the walkway (0.60 m vs 0.15 m) and the height of the walking surface (floor vs elevated: 0 m vs 0.60 m). Results indicated that although both younger and older adults altered their gait patterns under conditions of increased postural threat, the movement adaptations observed among older adults were substantially different to those adopted by younger adults. These age-dependent differences were strongly evidenced in the joint kinematics and the variability of the gait pattern within each condition. Our findings also indicated that when postural threat increased, the level of muscle activation throughout the gait cycle was altered in the distal musculature (gastrocnemius m. and tibialis anterior m.) among older adults only. Based on the age-related differences observed, we believe that the gait pattern alterations observed among younger and older adults reflect central set modifications to postural control that are mediated by a heightened anxiety imposed by the constraints of the testing conditions. Based on the age-dependent differences in the observed gait pattern modifications, it appears that the effects of anxiety on the control of locomotion are more pervasive for older adults than for younger adults.     

Natriuretic peptides, but not nitric oxide donors, elevate levels of cytosolic guanosine 3',5'-cyclic monophosphate in ependymal cells ex vivo

We examined how children with Spastic Hemiparetic Cerebral Palsy (SHCP) perform interceptive actions they experience in daily life. Children were required to walk towards and intercept a stationary ball or a moving ball, with either their impaired or non-impaired arm. In the stationary ball condition the child was free to determine the speed of their response (internal timing), whereas in the moving ball condition there was a restricted time available (external timing). It was found that the reach movements of the non-impaired arm were different to the impaired arm, and were characterized by some of the typical movement limitations imposed by SHCP. However, there was no evidence of increased contribution from trunk motion or a lengthening of reach movement time or deceleration time. Instead, there was a coordinated change with the walking kinematics, whereby the children spent proportionately more time slowing down as they approached the point of interception when reaching with the impaired arm. There were also several differences in the response when intercepting a moving ball compared to a stationary ball. When the timing constraints were imposed externally (moving ball) rather than internally (stationary ball), children reached with a reduced movement time and deceleration time, and an increased peak wrist velocity and elbow excursion. These adaptations to behaviour were necessary to deal with the restricted time available to make the interception in the moving ball condition compared to when the ball was stationary, and reveal how children with SHCP coordinate walking and reaching when performing natural interceptive actions.     

On the origin of the soleus H-reflex modulation pattern during human walking and its task-dependent differences

Recently, Brooke and colleagues have suggested "that the strong inhibition arising from passive movement about the knee and hip joints, lays down the base for the soleus H-reflex gain modulation seen during human gait." In particular stretch-evoked afferent activity from the quadriceps muscle was emphasized as the most important source of movement-induced inhibition of the H-reflex. To test this hypothesis we examined the kinematics and electromyographic (EMG) activity of the leg during human walking and correlated these with the modulation pattern of the soleus H-reflex. To further test the possible contribution of stretch-evoked quadriceps afferent activity to the soleus H-reflex modulation pattern during walking different walking gaits were studied. In one condition subjects were asked to walk with their knee locked in full extension by a rigid knee brace. In a second condition subjects were asked to walk backwards. During normal walking, the soleus H-reflex modulation pattern is strongly correlated with the EMG events of the soleus and tibialis anterior (TA), but not with hip, knee, or ankle angular displacement or velocity. When subjects walked with the knee locked in full extension, the amplitude of the H-reflex, its modulation pattern, and the task-dependent changes of its amplitude were the same as during normal walking. During backward walking, the H-reflex increases in late swing before activity of the soleus has begun and while the knee is flexing, an observation that highlights central control of the H-reflex amplitude. The effects of imposed flexion of the knee in passive subjects were also reexamined. The knee flexion imposed by the experimenter followed the same trajectory as that which occurred during the swing phase of the subject's step cycle. It was found that imposed knee flexions elicited a burst of TA EMG activity with an average latency of 81.6 ms (SD = 21 ms) in six out of eight subjects. Inhibition of the H-reflex, when it occurred, was associated with the occurrence of this burst. When subjects voluntarily flexed their right knee from an initial quiet standing posture, the inhibition of the soleus H-reflex began before flexion of the knee or that of any other leg segment. Once again the onset of inhibition was closely associated with the onset of activity in the TA. In the discussion section the present observations are examined in light of the predictions made by the movement-induced inhibition hypothesis of Brooke et al. It will be concluded that none of the predictions of this hypothesis were corroborated by present tests done during human walking. In consequence, we suggest that the modulation pattern of the H-reflex observed during normal human walking is centrally determined, as are the task-dependent differences of its amplitude (e.g., standing versus the stance phase of human walking).     

Comparison between preferred and energetically optimal transition speeds in adolescents

The primary focus of this investigation was to determine whether differences exist between the preferred transition speed (PTS) and the energetically optimal transition speed (EOTS) in a group of adolescents. Ten 11-, ten 13-, and ten 15-year-olds completed four testing sessions. Following 30 min of accommodation to treadmill walking and running (session 1), the PTS between walking and running was identified in session 2. In session 3, subjects walked on a level treadmill at 70%, 80%, 90%, 100%, and 110% of respective PTS, while in session 4, children ran on a level treadmill at 90%, 100%, 110%, 120%, and 130% of respective PTS. During the last 2 min of each 5-min walking and running bout, expired gas samples were collected in a meteorological balloon and analyzed to calculate and the EOTS between walking and running. Data analyses revealed that mean EOTS was significantly higher than mean PTS within each age group. Furthermore, when subjects changed gaits, the aerobic demand needed to run at the PTS was not lower than the measured while walking at the PTS. A moderately strong positive coefficient (r=0.71) between leg length and PTS was also observed. Taken together, these data suggest factors other than govern the walk–run transition in adolescent boys and girls. Electronic Publication     

Effects of optic flow on spontaneous overground walk-to-run transition

Perturbations of optic flow can induce changes in walking speed since subjects modulate their speed with respect to the speed perceived from optic flow. The purpose of this study was to examine the effects of optic flow on steady-state as well as on non steady-state locomotion, i.e. on spontaneous overground walk-to-run transitions (WRT) during which subjects were able to accelerate in their preferred way. In this experiment, while subjects moved along a specially constructed hallway, a series of stripes projected on the side walls and ceiling were made to move backward (against the locomotion direction) at an absolute speed of −2 m s⁻¹ (condition B), or to move forward at an absolute speed of +2 m s⁻¹ (condition F), or to remain stationary (condition C). While condition B and condition F entailed a decrease and an increase in preferred walking speed, respectively, the spatiotemporal characteristics of the spontaneous walking acceleration prior to reaching WRT were not influenced by modified visual information. However, backward moving stripes induced a smaller speed increase when making the actual transition to running. As such, running speeds after making the WRT were lower in condition B. These results indicate that the walking acceleration prior to reaching the WRT is more robust against visual perturbations compared to walking at preferred walking speed. This could be due to a higher contribution from spinal control during the walking acceleration phase. However, the finding that subjects started to run at a lower running speed when experiencing an approaching optic flow faster than locomotion speed shows that the actual realization of the WRT is not totally independent of external cues. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. K. De Smet and P. Malcolm contributed equally to this work.     

Motor asymmetries in the human newborn are state dependent, but independent of position in space

Human newborns have a preference for turning and maintaining the head to one side of the body. Most studies confirm a right-sided preference in supine. Few have addressed the state dependency of this lateral bias, and even fewer have examined whether it is also expressed in the semi-upright position. We investigated whether it varies as a function of behavioural state and position in space. Kinematic recordings of head movements were made with the newborn secured on a platform in the supine or semi-upright position, which alleviated biomechanical and postural constraints imposed by gravity. Newborns differed as to whether they had a vertex, Caesarean or breech delivery. The majority of infants maintained a right-sided preference in both positions, but it was strongly mediated by state. Delivery type did not account for any lateral bias. These findings provide convincing evidence that a lateral bias in movement and positioning of the head are reflections of active neural processes rooted in the regulation of state.     

Adjunctive behavior induced by different conditions of wheel running

Adjunctive behaviors such as licking, nose poking, rearing, grooming and locomotion induced by intermittent wheel running were observed in 12 female hooded rats. Animals were studied both under scheduled and free wheel conditions. Although all of the observed behaviors were emitted at high frequencies during scheduled wheel turning activity, only revolutions and rearings-locomotion increased significantly when compared to the free wheel condition when animals scheduled themselves. These data demonstrate again that adjunctive behavior can be produced in animals which are not deprived of food or water and support an explanation in terms of a nonspecific increase in motor excitability induced by the intermittance of schedule associated stimulation.     

When is Higher Level Cognitive Control Needed for Locomotor Tasks Among Patients with Parkinson’s Disease?

Turning has been implicated as a complex task that requires both motor and cognitive resources. Accumulating evidence shows that patients with Parkinson’s disease (PD) require more steps and more time to complete a turn, however, the role of the prefrontal cortex during turning is not clear. Forty nine patients with PD without freezing of gait (mean age 71.7?±?1.0 years; 67% men, disease duration 9.7?±?1.3 years) performed motor and cognitive tests. Prefrontal activation, specifically in Brodmann area 10 (BA10), during turning and usual walking was measured using functional near infrared spectroscopy (fNIRS). The patients with PD were further divided into two subgroups with high and low functional status based on limitations in community ambulation. General Linear Model analysis adjusted for age, gender, disease duration and turn duration was used to assess differences between tasks and subgroups of patients with PD. In addition, Pearson’s correlation was performed to assess association between BA10 activation and motor and cognitive scores. Activation in BA10 increased during walking (p?<?0.001), while it decreased during turning (p?=?0.006). A comparison between the two subgroups of patients with PD revealed that patients with relatively better ambulation decreased prefrontal activation during turning, as compared to patients with relatively worse ambulation (p?<?0.001). These findings are the first to show that BA10 plays a different role during turning and walking and that ambulation status may alter BA10 activation during turning. Higher prefrontal activation during turning in the subgroup of patients with relatively worse ambulation may reflect a compensatory attempt at improving performance.     

Lumbar back muscle activity during locomotion: effects of voluntary modifications of normal trunk movements

The mechanisms of adaptation of the trunk to changed mechanical conditions were studied during locomotion in man. The myoelectrical (EMG) activity in lumbar back muscles and the movements of the trunk were recorded in nine healthy subjects during walking and running on a motor-driven treadmill. Two different types of voluntary modifications of the movement pattern were used: (1) The trunk was kept in an extreme forward or backward tilted position. In both these situations the basic EMG pattern with two periods of activity per stride cycle was maintained during walking, whereas a major shift relative to the stride cycle (25% of the stride cycle duration) occurred in running with the trunk tilted backwards. The synchrony of the back muscle activation at both sides increased when locomotion was performed with the trunk tilted forwards. The relative duration of the EMG bursts was similar to normal locomotion and corresponded to 15-26% of the stride cycle duration in walking and 23-37% in running. (2) In the other type of modification the subjects were instructed to exaggerate the angular trunk movements either in the sagittal or in the frontal plane. The basic EMG pattern and phase relationships remained in most cases unchanged. One exception was running with exaggerated lateral movements, in which only one period of back muscle activity per stride cycle was observed. The relative duration of the bursts was longer in trials with exaggerated trunk movements as compared to normal locomotion. In walking and running with the trunk tilted forwards or backwards the lumbar back muscles were not always involved as prime movers of the trunk. This was in contrast to the more dynamic situations, in which the back muscle activity appeared to be directly involved in braking and reversing the exaggerated trunk movements.     

Locomotion through apertures when wider space for locomotion is necessary: adaptation to artificially altered bodily states

The objective of this study is to describe the adaptability of the central nervous system to safely cross a narrow aperture when the space required for passage is transiently extended with external objects under different locomotor constraints. In one of four locomotion forms (normal walking, walking while holding a 63-cm horizontal bar with or without rotating the shoulders to cross a door opening, and wheelchair use), nine participants were asked to pass through an aperture created by two doors (the relative aperture widths were 1.02, 1.10, and 1.20 times their maximum horizontal dimension under each form of locomotion) without a collision. The kinematic analyses showed that, when the participants rotated their shoulders while walking and holding a bar, virtually the same locomotor patterns as those during normal walking were observed: shoulder rotation was regulated well in response to the width of an aperture, and no collisions occurred. When shoulder rotations were restricted while walking and holding a bar or using a wheelchair, a large reduction in the speed of movement was observed as the participants approached the door, and, furthermore, the modulation in speed was dependent on the width of the aperture. In addition, the participants crossed at the center of aperture more accurately; nevertheless, collision sometimes occurred (more frequently, during wheelchair use). These findings reveal that movement constraints on shoulder rotation are likely to be a critical factor in determining whether quick and successful adaptation takes place.     

Coordinating degrees of freedom during interceptive actions in children

The aim of the experiment was to examine how children coordinate the degrees of freedom of the arm and trunk when performing interceptive actions that correspond to daily life activities. For that purpose, children were required to reach and grasp a stationary ball while standing (condition C1), a stationary ball while walking (C2), and a moving ball while standing (C3). The resulting movements were measured in world-centered and body-centered coordinates, and then subjected to three-dimensional kinematic analysis. The different coordinate frames of reference were used to determine the interaction between arm and trunk movements. Children adapted their coordination in the two moving conditions (C2 and C3) by decelerating longer towards the ball and exhibiting more interaction between the arm and trunk movements than in the stationary condition (C1). These results indicate that, like adult participants, children adapt to the constraints imposed by complex, interceptive actions by recruiting additional degrees of freedom of the trunk, which are coordinated with the hand to produce a movement that preserves an appropriate level of impact at hand/object collision.     

Modulation of multisegmental monosynaptic responses in a variety of leg muscles during walking and running in humans

Motor responses evoked by stimulating the spinal cord percutaneously between the T11 and T12 spinous processes were studied in eight human subjects during walking and running. Stimulation elicited responses bilaterally in the biceps femoris, vastus lateralis, rectus femoris, medial gastrocnemius, soleus, tibialis anterior, extensor digitorum brevis and flexor digitorum brevis. The evoked responses were consistent with activation of Ia afferent fibres through monosynaptic neural circuits since they were inhibited when a prior stimulus was given and during tendon vibration. Furthermore, the soleus motor responses were inhibited during the swing phase of walking as observed for the soleus H-reflex elicited by tibial nerve stimulation. Due to the anatomical site and the fibre composition of the peripheral nerves it is difficult to elicit H-reflex in leg muscles other than the soleus, especially during movement. In turn, the multisegmental monosynaptic responses (MMR) technique provides the opportunity to study modulation of monosynaptic reflexes for multiple muscles simultaneously. Phase-dependent modulation of the MMR amplitude throughout the duration of the gait cycle period was observed in all muscles studied. The MMR amplitude was large when the muscle was activated whereas it was generally reduced, or even suppressed, when the muscle was quiescent. However, during running, there was a systematic anticipatory increase in the amplitude of the MMR at the end of swing in all proximal and distal extensor muscles. The present findings therefore suggest that there is a general control scheme by which the transmission in the monosynaptic neural circuits is modulated in all leg muscles during stepping so as to meet the requirement of the motor task.     

The moving platform aftereffect: limited generalization of a locomotor adaptation

We have recently described a postural after-effect of walking onto a stationary platform previously experienced as moving, which occurs despite full knowledge that the platform will no longer move. This experiment involves an initial baseline period when the platform is kept stationary (BEFORE condition), followed by a brief adaptation period when subjects learn to walk onto the platform moving at 1.2 m/s (MOVING condition). Subjects are clearly warned that the platform will no longer move and asked to walk onto it again (AFTER condition). Despite the warning, they walk toward the platform with a velocity greater than that observed during the BEFORE condition, and a large forward sway of the trunk is observed once they have landed on the platform. This aftereffect, which disappears within three trials, represents dissociation of knowledge and action. In the current set of experiments, to gain further insight into this phenomenon, we have manipulated three variables, the context, location, and method of the walking task, between the MOVING and AFTER conditions, to determine how far the adaptation will generalize. It was found that when the gait initiation cue was changed from beeps to a flashing light, or vice versa, there was no difference in the magnitude of the aftereffect, either in terms of walking velocity or forward sway of the trunk. Changing the leg with which gait was initiated, however, reduced sway magnitude by approximately 50%. When subjects changed from forward walking to backward walking, the aftereffect was abolished. Similarly, walking in a location other than the mobile platform did not produce any aftereffect. However, in these latter two experiments, the aftereffect reappeared when subjects reverted to the walking pattern used during the MOVING condition. Hence, these results show that a change in abstract context had no influence, whereas any deviation from the way and location in which the moving platform task was originally performed profoundly reduced the size of the aftereffect. Although the moving platform aftereffect is an example of inappropriate generalization by the motor system across time, these results show that this generalization is highly limited to the method and location in which the original adaptation took place.     

Selection for aerobic capacity affects corticosterone, monoamines and wheel-running activity

A positive genetic relationship between aerobic capacity and voluntary exercise has been suggested from earlier studies of mice selected for increased wheel-running activity. To further investigate the relationship between aerobic capacity and exercise behavior, wheel-running activity was studied in female rats bidirectionally selected for intrinsic aerobic capacity (high capacity runners - HCR; low capacity runners - LCR). Aerobic capacity was measured using a forced treadmill paradigm; the subpopulations of animals used in this experiment exhibited a 471% difference in endurance capacity. Rats were housed individually, with or without access to running wheels. Wheel-running activity was recorded and analyzed from weeks two through seven during an eight-week trial to determine voluntary activity levels. HCR animals exhibited 33% greater total wheel-running distance per day compared to LCR rats (16,838.7+1337.30 m versus 12,665.8+893.88 m), which was due to the HCR rats exhibiting increases in both running speed and duration over LCR rats. Differences in the intermittency of wheel running were also observed. HCR rats engaged in more bouts of running per day than LCR rats, and trended towards running faster, for more time, and for longer distances during bouts of running than LCR rats. Following the running trial, measurement of plasma corticosterone concentration and striatal dopaminergic activity showed differences between HCR and LCR rats, suggesting a divergence of physiological systems that could potentially influence locomotor behaviors in these lines. These results are consistent with earlier work, and suggest an evolutionarily conserved relationship between physiological capacity and behavioral activity of exercise.     

The effects of listening to music or viewing television on human gait

This paper presents a two-part study with walking conditions involving music and television (TV) to investigate their effects on human gait. In the first part, we observed seventeen able-bodied adults as they participated in three 15-minute walking trials: (1) without music, (2) with music and (3) without music again. In the second part, we observed fifteen able-bodied adults as they walked on a treadmill for 15 min while watching (1) TV with sound (2) TV without sound and (3) TV with subtitles but no sound. Gait timing was recorded via bilateral heel sensors and center-of-mass accelerations were measured by tri-axial accelerometers. Measures of statistical persistence, dynamic stability and gait variability were calculated. Our results showed that none of the considered gait measures were statistically different when comparing music with no-music trials. Therefore, walking to music did not appear to affect intrinsic walking dynamics in the able-bodied adult population. However, stride interval variability and stride interval dynamics were significantly greater in the TV with sound walking condition when compared to the TV with subtitles condition. Treadmill walking while watching TV with subtitles alters intrinsic gait dynamics but potentially offers greater gait stability.     

Effects of sex and laterality on the rotatory swimming behavior of normal mice

Clockwise and counterclockwise full turns are commonly used to assess lateralization in circling behavior. Although previous studies have reported that the rotatory swimming (ROSW) test is simple and reliable, little is known about lateralization of turns lower than 360 degrees and the amount of turning close to the wall, and even less is known about alternation of direction during a session. Here we investigated the effects of consistency of laterality and sex on 30 degree turns in center and in periphery of the swimming apparatus, and on alternation of direction upon three sessions. Approximately 80% of the turns occurred when mice swam along the wall. In side-consistent turners, this suggests the existence of an intrinsic sensorimotor asymmetry that determines the adhesion to the preferred side. Regarding categorization of side preferences, there was a high percentage of agreement between center and periphery, as well as between full turns and extra 30 degree turns (30 degree turns that do not contribute to full turns). Therefore, behavioral asymmetry in the ROSW can be assessed using 30 degree turns. There was no significant directional bias in the population, and side preference was found to be independent of sex. By contrast, after the second minute of each session, males exhibited a significantly higher number of reversal of direction (RD) as well as a higher number of RD per turn than females. The amount of RD presented by each animal is not predicted by the animal's side preference. Thus, RD is independent of preferred side of turning and depends on sex.