中药槐黄软膏抗炎镇痛作用的实验研究

我们对中药槐黄软膏的抗炎镇痛作用进行了实验研究，现将结果报告如下。     

生肌软膏治疗皮肤溃疡愈合的实验研究

目的：研究生肌软膏对皮肤溃疡愈合的作用机理。方法：ccV大鼠36只，制成溃疡模型，分为2组，分别涂用生肌软膏和医用凡士林外用制剂，于给药的第3、7、11d取溃疡处的全层组织，进行组织学观察。结果：生肌软膏组的溃疡面肉芽组织健康，毛细血管丰富，巨噬细胞较多，并有血管内皮生长因子（VEGF）、增殖细胞核抗原（PCNA）免疫组化检测，生肌软膏组的阳性细胞率明显高于凡士林组的阳性细胸率而支持。两组比较差别显著（P＜0．05）。结论：中药外用制剂在治疗皮肤溃疡中有比较明显的优势，不仅可以促进溃疡的生长、愈合，还可以缩短愈合时间。     

自制外用软膏治疗痔的临床观察

为观察自制外用软膏治疗痔的临床疗效及安全性,为痔的临床治疗用药寻求一种新的有效选择。将符合纳入标准的128例患者随机分为治疗组和对照组,其中治疗组63例,对照组65例,治疗组使用自制外用软膏外用治疗。每日早晚各一次．10d为1个疗程。治疗1个疗程后观察内痔、外痔、混合痔的临床疗效。治疗前后主要观察指标为便血、疼痛、脱出、水肿、坠胀,并对疗效进行统计学分析。结果显示．两组患者疗效比较均有显著性差异（P〈0．05）,治疗组优于对照组。结果表明．自制外用软膏对各种培的主要临床症状均有较好的改善作用。     

京万红软膏治疗糖尿病慢性创面的实验研究

目的：探讨京万红软膏促进糖尿病小鼠慢性创面愈合的效果及机制.方法：清洁级、同周龄雄性C57小鼠54只,随机分为京万红软膏组、对照药物组和空白对照组,每组18只.采用连续3 d腹腔注射1%链脲佐菌素60 mg/kg的方法建立糖尿病小鼠模型,然后在小鼠背部制作直径1 cm的圆形全层皮肤缺损创面.创面形成第2日开始,京万红软膏组创面涂抹京万红软膏,厚度0.5 cm; 对照药物组创面涂抹相同厚度复方磺胺嘧啶锌凝胶剂;空白对照组不给药.3组创面均以凡士林纱布覆盖,每日换药1次.于给药后第3、7、10、17、21天,用塑料透明膜描记创面大小,计算创面愈合率.于给药后第7、17天各组分别随机选取3只小鼠处死,观察创面及创缘2 mm范围内全层皮肤及肉芽组织的大体形态变化及组织病理学变化.结果：给药后7 d,两药物治疗组创面愈合率均有所升高,但3组间差异无显著性（P〉0.05）.给药后10、17和21 d,对照药物组创面愈合率[（81.00±0.85）%,（95.00±0.29）%,（97.00±0.37）% ]明显高于空白对照组[（77.00±1.35）%,（87.00±1.17）%,（90.00±0.96）%,P〈0.05],而京万红软膏组愈合率[（85.00±1.93）%,（100.00±0）%,（100.00±0）% ]明显高于对照药物组（P〈0.05）.治疗17 d京万红软膏组创面全部愈合,而对照药物组和空白对照组在治疗后21 d仍未全部愈合.组织病理学观察发现,京万红软膏较对照药物复方磺胺嘧啶锌凝胶剂能更早使炎症减轻、创面上皮化,而且表皮细胞复层分化良好,新生胶原排列整齐,创面组织结构恢复正常化明显,显示具有更好的愈合质量.结论：京万红软膏对于糖尿病慢性创面有良好的促进愈合作用.     

自制东方一号软膏治疗溃疡期压疮

目的 探讨东方一号软膏治疗溃疡期压疮的效果.方法 将55例(68处)溃疡期压疮患者随机分成两组,在彻底清疮基础上,对照组26例(33处)采用凡士林油纱布覆盖创面,观察组29例(35处)用东方一号软膏涂于纱布覆盖.结果 观察组治疗效果显著优于对照组,疮面愈合时间显著短于对照组(均P＜0.01).结论 东方一号软膏治疗溃疡期压疮患者效果显著.     

Angiostatin基因治疗人肝癌裸鼠移植瘤的实验研究

目的:研究Angiostatin基因治疗对人肝癌裸鼠皮下移植瘤的抑制作用及其相关机理。方法:使用人原发性肝癌细胞株SMMC-7721建立人肝癌裸鼠皮下移植瘤动物模型,质粒用脂质体DOTAP介导转染细胞。将荷瘤裸鼠随机分为两组,分别注射质粒PcDNA3、Angiostatin/PcDNA3,观察两组动物的肿瘤生长曲线,检测肿瘤的Angiostatin、VEGF、HIF-1α表达和微血管密度(MVD),利用TUNEL染色法行原位细胞凋亡分析。结果:Angiostatin基因治疗在早期具有抑制肿瘤生长的作用,大约1周后肿瘤以更快的速度生长并迅速赶上空质粒对照组肿瘤;Angiostatin基因治疗组的肿瘤组织中有An-giostatin的局部高表达,MVD(24.8±2.8)低于空质粒对照组(30.2±4.1)(P〈0.05)。肿瘤组织中HIF-1α蛋白局部高表达,VEGF表达高于空质粒对照组,细胞凋亡指数(2.87±0.48)高于空质粒对照组(1.55±0.43)(P〈0.01)。结论:Angiostatin基因治疗对人肝癌裸鼠皮下移植瘤的生长具有一定的抑制作用,肿瘤对Angiostatin基因治疗可以产生耐受性。     

肛泰软膏配合中药外洗治疗肛裂40例

近年来,笔者采用肛泰软膏局部外用配合中药外洗治疗肛裂40例,取得满意效果,现报告如下。临床资料：本组男25例,女15例;年龄5～62岁,平均31．27岁;发病时间1d至4年不等。     

三黄软膏不同时段外敷防治诺维本致静脉炎的实验研究

目的探讨不同时段三黄软膏外敷防治诺维本致兔耳静脉炎的效果,以明确三黄软膏外敷防治静脉炎的最佳使用时间。方法建立诺维本所致化疗性静脉炎的兔耳模型,随机分为A、B、C三组。分别在静脉注射诺维本后即刻、24h、48h用三黄软膏外敷干预,于第7天进行观察和局部病理切片,以评价静脉损伤程度。结果 A组静脉炎发生率最低,病理损伤程度最轻,其防护效果优于B、C组,差异有统计学意义(P<0.05,P<0.01)。结论三黄软膏越早外敷防治静脉炎效果越好。     

硝酸银软膏对Ⅱ度烧伤创面治疗作用的多中心临床研究

目的观察硝酸银(AgNO3)软膏对浅Ⅱ、深Ⅱ度烧伤创面的治疗效果,并评价其药物不良反应。方法选择80例浅Ⅱ度和40例深Ⅱ度烧伤患者,进行多中心、随机、阳性药物平行对照和同体试验研究(共4个中心,每个中心30例)。将患者创面按用药不同分为AgNO3组和磺胺嘧啶银(SD-Ag)组,观察各组创面完全愈合时间、指定时相点下创面愈合率、创面细菌培养情况、药物疗效和安全性、药物对创面的刺激性等。结果浅Ⅱ度创面:AgNO3组完全愈合时间为(9．5±2．7)d, SD-Ag组为(10．8±3．4)d,用药后7 d创面愈合率分别为(77．9±20．5)％及(67．3±22．6)％;深Ⅱ度创面:AgNO3组完全愈合时间为(21．5±4．8)d,SD-Ag组为(23．3±6,4)d,用药后20 d创面愈合率分别为(86．6±15．9)％及(78．5±17．7)％。同等深度烧伤创面上述各项数据两组间比较,差异均有统计学意义(P<0．01)。同等深度烧伤创面AgNO3组与SD-Ag组比较,具有同样明显的杀菌作用,但前者对创面的刺激性更小。结论AgNO3软膏是一种可用于浅Ⅱ、深Ⅱ度烧伤创面的有效、安全的外用药。     

冰黄肤乐软膏外涂加亚甲蓝局部注射治疗肛周湿疹40例

肛周湿疹是一种常见的非传染性皮肤病,发病原因较多,临床治疗比较困难。我院采用冰黄肤乐软膏外涂加亚甲蓝局部注射治疗肛周湿疹40例,取得满意疗效,总结报道如下。     

防疤烧伤膏对大鼠创面愈合过程中TGFβ1基因表达的影响

目的观察防疤烧伤膏对大鼠伤口愈合过程中TGFβmRNA表达及蛋白含量的影响.方法采用大鼠皮肤切除致伤模型,创面外用防疤烧伤膏,并采用碱性成纤维细胞生长因子(basic fibroblast growth,bFGF)和空白处理作为阳性和阴性对照.伤后3、7、14d取创面组织,经10%中性福尔马林固定,石蜡包埋,切片后采用免疫组化和原位杂交的方法检测伤口组织中TGFβ1mRNA和其蛋白含量的变化.结果与空白对照组相比,防疤烧伤膏处理后的创面组织细胞内TGFβ1的mRNA和蛋白含量明显减少.结论防疤烧伤膏在促进创面愈合的同时,能通过抑制创面组织细胞中TGFβ1的基因表达和蛋白含量的升高,因此,可能使伤口愈合后的瘢痕形成减少.     

踝关节炎药膏涂擦疗法的临床疗效观察

2009年11月～2010年4月,我科门诊收治踝关节炎患者153例,采用不同方法治疗,笔者观察临床疗效,报道如下。1材料与方法1.1研究对象2009年11月～2010年4月期间获随访的门诊患者153例,     

The effect of capsaicin ointment on skin for the survival of a cutaneous flap

Recent studies have shown that capsaicin has three major effects: vasodilation, platelet disaggregation and pain reduction. In this study, vasodilation and platelet disaggregation effects were compared using an ointment (Surgilube) containing capsaicin. Forty rats were divided into four groups of 10. A distally-based dorsal skin flap was raised and sutured back into place. The circulatory territory of the flap was measured by a fluorescein technique. In group A, Surgilube was applied on and around the flap as a control for the surgical technique and ointment base. Surgilube containing capsaicin (0.2%) was applied on the surface of the flap in group B to observe platelet disaggregation effects, and around the perimeter (avoiding the incision) of the flap in group C to observe vasodilation effects. Group D received the capsaicin ointment both on and around the flap. Survival distance in millimeters was calculated as the difference from the distal end of the fluorescein stain to the distal margin of flap survival at 7 days after surgery. Analysis for difference among the groups was assessed by the method of Kruskall-Wallis followed by Mann Whitney tests for differences between the two group. The survival distances (mm) for the groups were, in order of better survival, group C (19.9), group D (14.6), group B (9.7) and group A (7.2). The improvement in survival related to the control group was statistically significant for group C (P=0.002), with group D also approaching significance (P=0.052). Furthermore, the survival distance of group C was significantly better than that of group B (P=0.004). Capsaicin was most effective in promoting skin flap survival when applied around the flap. This suggests that the vasodilation effect in flap survival is stronger than that of platelet disaggregation.     

The preventive effect of cimetidine on producing stress ulcer in rats

Stress ulcers were formed by a standard method after fasting and restraining each rat in a performed conduit tube and placing them in a cold room head inverted at 4°C for three hours. The severity of the ulcer was graded into four groups; Grade 0: no ulcer, Grade 1: 1–10 ulcers, Grade 2: 11–20 ulcers, Grade 3: more than 21 ulcers. The animals were divided into six groups: Group I: no stress, no treatment; Group II: stressed but not treatment; Group III: pretreated with saline and stressed; Group IV: pretreated with antacid and stressed; Group V: pretreated with Cimetidine and stressed; Group VI: stressed and then post-treated with Cimetidine. In group II 47 out of 49 (96 per cent) developed ulcers compared to no ulcers in group I. In group V, 13 out of 38 (34 per cent) and in group IV, 13 out of 20 (65 per cent) developed ulcers. In group V, not only the incidence was reduced markedly but also the severity of ulcer was significantly less. However, post-treatment with cimetidine was not as effective as pre-treatment. Cimetidine, therefore, seems to be very effective in preventing stress induced ulcers in rat when instilled in the stomach before the stress.