中性粒细胞胞外诱捕网与牙周炎高凝状态的相关性研究

目的 探究中性粒细胞胞外诱捕网(neutrophil extracellular traps,NETs)对重度牙周炎患者凝血指标的影响.方法 纳入20例重度牙周炎患者作为试验组,20例健康人作为对照组,记录牙周指标、凝血指标.ELISA测定外周血中NETs标志物cf-DNA和MPO-DNA的含量,免疫荧光显微镜观察不同...     

吸烟对牙周炎基础治疗后龈沟液TNF-α和IL-10的影响

目的通过检测吸烟与不吸烟慢性牙周炎患者牙周基础治疗前、后龈沟液肿瘤坏死因子-α和白细胞介素-10的变化,分析吸烟对治疗效果的影响。方法选择男性慢性牙周炎患者27例,分为吸烟组12例和不吸烟组15例。在牙周基础治疗前和治疗后1个月分别测定龈沟液TNF-α和IL-10水平以及牙周临床指标。结果基础治疗前、后牙周探诊深度的下降值吸烟组低于不吸烟组（P〈0.05）。临床附着丧失的下降值吸烟组与不吸烟组差异无统计学意义（P〉0.05）。TNF-α浓度的下降值吸烟组低于不吸烟组（P〈0.05）。IL-10浓度的升高值吸烟组低于不吸烟组（P〈0.05）。结论吸烟影响牙周基础治疗效果。     

中性粒细胞细胞外陷阱网与牙周炎的相关性研究进展

中性粒细胞是机体防御系统的成员之一,在免疫反应中发挥着重要作用。中性粒细胞细胞外陷阱网(NET)是新近发现的中性粒细胞发挥作用的一种形式,除了能够诱获并杀灭病原体而发挥作用之外,还可能对机体产生直接或者间接的损伤。NET不仅出现在牙周组织中,还与牙周致病菌息息相关。本文对NET与牙周炎的相关性研究进行综述。     

牙周非手术治疗对重度牙周炎病人TNF-α和血管内皮功能的影响

目的:探讨牙周非手术治疗对重度牙周炎病人血管内皮功能和TNF-α水平的影响。方法:选择20名重度牙周炎病人,比较牙周非手术治疗前及治疗后3个月的出血指数、探诊深度、附着水平、菌斑指数、TNF-α、血流介导的肱动脉扩张和硝酸甘油介导的肱动脉扩张。结果:牙周非手术治疗后3个月,所有病人的牙周临床指标(探诊深度,附着丧失,出血指数和菌斑指数)均较治疗前有明显好转(P<0.05);TNF-α水平较治疗前明显下降(P<0.05);血流介导的肱动脉扩张率明显升高(P<0.05);而硝酸甘油介导的肱动脉扩张率变化不明显。结论:治疗牙周炎有助于改善血管内皮功能和降低TNF-α水平。     

IL-10多态性对牙周炎患者牙周微生态的影响

目的：探讨IL-10多态性对牙周炎患者牙周微生态的影响。方法：选取2018年6月—2019年3月淄博市第一医院收治的牙周炎患者作为实验组（n=79）,按照牙周炎类型分为中度牙周炎（n=47）与重度牙周炎（n=32）。另选择同期来院体检的牙周健康人作为对照组（n=50）。采用实时荧光定量PCR技术检测所有研究对象龈下牙菌斑中细菌定植水平;抽取静脉血,通过PCR技术检测IL-10中的-1082、-819、-592位点上的基因型及等位基因频率。通过多因素分析,探讨IL-10不同基因型与龈下牙菌斑中细菌定植水平的关系。采用SPSS 22.0软件包对数据进行统计学分析。结果：中度牙周炎患者伴放线放线杆菌(Actinomycetes actinomycetes,A.a)定植量显著低于重度牙周炎患者（P<0.05）;中度牙周炎与重度牙周炎患者牙龈卟啉单胞菌（Porphyromonas gingivalis,P.g）及A.a定植量均显著高于对照组（P<0.05）。中度牙周炎患者中,-819位点的CC基因型比例显著低于对照组,TT基因型比例显著高于对照组（P<0.05）;-819位点CC、TC、TT基因型比例与重度牙周炎患者相比无显著差异（P>0.05）;-592位点的CC基因型比例显著低于对照组,AA基因型比例显著高于对照组（P<0.05）。中度牙周炎中,-592位点的AA基因型患者A.a定植量显著高于本组AC基因型患者（P<0.05）;-819位点的TT基因型患者A.a定植量显著高于本组TC基因型患者（P<0.05）。多因素分析显示,中度牙周炎中IL-10-592-AA基因型、-819-TT基因型与A.a定植水平密切相关（P<0.05）。结论：IL-10基因多态性是中度牙周炎患者龈下微生态环境的影响因素,其中,IL-10-592-AA基因型、-819-TT基因型与A.a定植水平相关性最明显。     

人参皂甙Rg-1对牙周炎大鼠牙周组织中TNF-α、IL-1β表达的影响

目的：检测牙周炎大鼠牙周组织中肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）、白介素-1β（in-terleukin-1β,IL-1β）水平的变化,探讨人参皂甙Rg-1对其表达的影响及治疗牙周炎的机理。方法：选用100只体重160-200g Wistar大白鼠,随机分成10组,每组10只。A组：正常对照组,与F组同时处死。B-J组建立牙周炎大鼠模型。B-E组：人参皂甙Rg-1治疗组,给药后第1、2、3、4周末处死。F-J组：牙周炎对照组,于造模成功后第0、1、2、3、4周末处死。取大鼠上颌磨牙牙周组织标本作切片,光镜观察,采用免疫组织化学法检测TNF-α、IL-1β水平变化,进行统计学分析。结果：F组牙周组织中TNF-α、IL-1β表达明显高于A组（P〈0.05）;各时间段牙周炎治疗组牙周组织中TNF-α、IL-1β表达明显低于相应的牙周炎组（P〈0.05）。结论：人参皂甙Rg-1可以抑制实验性牙周炎大鼠牙周组织中TNF-α、IL-1β的表达,具有治疗牙周炎的作用。     

罗格列酮对牙周炎大鼠牙龈脂联素受体表达的影响

目的:探讨罗格列酮(ROS)对牙周炎大鼠牙龈脂联素受体1(AdipoRl) mRNA、脂联素受体2(AdipoR2) mRNA和TNF-α等炎症因子表达的影响及在牙周炎中对牙槽骨保护的潜能.方法:50只SD大鼠随机分为5组(n=10),大鼠不干预处理作为空白对照,40只大鼠用于制作牙周炎模型后分别用蒸馏水(牙周炎组)、1、3、10 mg/kg ROS(低、中、高剂量组)灌胃1次/d,持续4周.然后取样,RT-PCR测定牙龈组织AdipoR1和AdipoR2 mRNA表达水平,ELISA测牙龈组织TNF-α、MMP-9和血浆脂联素浓度,标准化的数码摄影测量釉牙骨质界到牙槽骨嵴顶(CEJ-A)距离.结果:牙周炎组和空白对照组相比,牙龈组织AdipoR1和AdipoR2的mRNA表达水平显著降低(P＜0.01),血浆脂联素水平无差异(P＞0.05),TNF-α和MMP-9浓度显著上升(P＜0.01).和牙周炎组相比,低、中、高剂量治疗组牙龈组织AdipoRl mRNA表达均升高(P＜0.05),TNF-α浓度显著降低(P＜0.01);中、高剂量治疗组牙龈组织AdipoR2 mRNA表达显著升高(P＜0.01),MMP-9浓度显著降低(P＜0.01),血浆脂联素浓度升高(P＜0.05),牙槽骨吸收量显著降低(P＜0.01).结论:ROS可能通过上调牙龈组织中AdipoR1和AdipoR2 mRNA表达水平,降低TNF-α、MMP-9浓度,缓解牙周组织炎症,降低牙槽骨吸收.     

持续正压通气对OSAHS合并牙周炎患者唾液TNF-α和IL-10含量影响

目的:探讨持续正压通气(continuous positive airway pressure,CPAP)治疗对阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome,OSAHS)合并牙周炎患者唾液炎症介质的影响.方法:将本院2016年1月～2017年12月收...     

The role of phagocytosis,oxidative burst and neutrophil extracellular traps in the interaction between neutrophils and the periodontal pathogen Porphyromonas gingivalis

Neutrophils are regarded as the sentinel cells of innate immunity and are found in abundance within the gingival crevice. Discovery of neutrophil extracellular traps (NETs) within the gingival pockets prompted us to probe the nature of the interactions of neutrophils with the prominent periopathogen Porphyromonas gingivalis. Some of the noted virulence factors of this Gram‐negative anaerobe are gingipains: arginine gingipains (RgpA/B) and lysine gingipain (Kgp). The aim of this study was to evaluate the role of gingipains in phagocytosis, formation of reactive oxygen species, NETs and CXCL8 modulation by using wild‐type strains and isogenic gingipain mutants. Confocal imaging showed that gingipain mutants K1A (Kgp) and E8 (RgpA/B) induced extracellular traps in neutrophils, whereas ATCC33277 and W50 were phagocytosed. The viability of both ATCC33277 and W50 dwindled as the result of phagocytosis and could be salvaged by cytochalasin D, and the bacteria released high levels of lipopolysaccharide in the culture supernatant. Porphyromonas gingivalis induced reactive oxygen species and CXCL8 with the most prominent effect being that of the wild‐type strain ATCC33277, whereas the other wild‐type strain W50 was less effective. Quantitative real‐time polymerase chain reaction revealed a significant CXCL8 expression by E8. All the tested P. gingivalis strains increased cytosolic free calcium. In conclusion, phagocytosis is the primary neutrophil response to P. gingivalis, although NETs could play an accessory role in infection control. Although gingipains do not seem to directly regulate phagocytosis, NETs or oxidative burst in neutrophils, their proteolytic properties could modulate the subsequent outcomes such as nutrition acquisition and survival by the bacteria.     

�ܸ��Ͻ𿾴�ȫ���޸��Ի���������֯Ӱ����о�

目的探讨钴铬合金烤瓷全冠修复对患牙牙周组织的影响。方法选择2007年4月至2008年10月来沈阳市沈河区牙病防治所应用钴铬合金烤瓷全冠修复的患者39例(106颗患牙),修复前和修复后6、12个月分别进行常规龈沟出血指数(SBI)检查,龈沟液(GCF)称量,GCF中白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)质量浓度的检测。结果修复前牙的患者患牙SBI、GCF质量、GCF中IL-1β和TNF-α的质量浓度修复前与修复后6个月比较差异无统计学意义(P>0.05),与修复后12个月比较差异有统计学意义(P<0.05);修复后牙的患者患牙SBI、GCF质量修复前与修复后6个月比较差异无统计学意义(P>0.05),与修复后12个月比较差异有统计学意义(P<0.05);修复后牙的患者患牙GCF中IL-1β和TNF-α的质量浓度修复前与修复后6个月和修复后12个月比较差异均无统计学意义(P>0.05)。结论采用钴铬合金烤瓷全冠修复前牙,修复时间超过12个月时,对SBI、GCF质量和GCF中IL-1β和TNF-α的质量浓度有显著影响;修复后牙,修复时间超过12个月时,对SBI、GCF质量有显著影响,而对GCF中IL-1β和TNF-α的质量浓度无明显影响。     

Interrelationship of inflammation and tooth mobility (trauma) in pathogenesis of periodontal disease

Abstract A series of studies has investigated interactions between periodontal trauma and marginal periodontitis in relation to the initiation, progression and treatment of periodontal disease. Lesions of trauma in the periodontal ligament consequent to either single or jiggling displacing forces result in morphologic alterations in the ligament and alveolar bone. These changes do not initiate the loss of connective tissue attachment characteristic of marginal periodontitis. Studies conducted in squirrel monkeys and beagle dogs in which jiggling forces have been produced subjacent to an established marginal periodontitis reported increased loss of alveolar bone, but the accelerated loss of attachment which occurred in the dog model did not occur in the monkey model. To clarify the relative importance of inflammation and tooth mobility in the treatment of advanced periodontal disease, periodontal response was evaluated after removing traumatic and/or inflammatory components. Elimination of trauma in the presence of existing marginal inflammation did not reduce tooth mobility or increase bone volume. Osseous regeneration and decreased tooth mobility occurred after resolving both components, however, similar findings occurred after resolving inflammation in the presence of continued tooth mobility. Resolution of marginal inflammation is of prime importance in the management of periodontal disease. After resolution of inflammation, bone regeneration may occur around mobile teeth and, furthermore, any residual tooth mobility does not result in increased loss of connective tissue attachment.     

Different treatment strategies are applied to patients with the same periodontal status in general dentistry

Objective. To analyse how general dental practitioners (GDPs) and dental hygienists judge and plan to treat patients with different periodontal conditions. Materials and methods. Seventy-seven GDPs and 50 dental hygienists in a Swedish county, Halland, participated in a questionnaire study. The response rate was 94%. The questionnaire consisted of four simulated patient cases and an attached answer sheet. The patient cases had different periodontal status, ranging from healthy to moderate bone loss with general inflammation. The clinicians judged the periodontal status as healthy or diseased. If judged as diseased the clinicians suggested a diagnosis, selected treatment options and estimated the number of treatment sessions for each patient case. The clinicians were compared to each other regarding their judgement, as healthy or diseased, diagnostics and treatment. Results. Three out of four patients were judged both as healthy and diseased by different clinicians. If judged as diseased the patients were diagnosed as having gingivitis or periodontitis. Regardless of the clinicians' former judgement and diagnostics there were no differences (p > 0.05) in the selected treatment options but there was a difference (p < 0.05) in the suggested number of treatment sessions. Conclusions. Clinicians' judgement of the same periodontal condition, as healthy or diseased, varies, which partly results in different treatment decisions considering the number of treatment sessions. The suggested number of treatment sessions varied also between clinicians even if they judged and diagnosed the condition likewise. The willingness to treat and suggested treatment options were not influenced by the variation in judgement and diagnostics.