母鸡油制三七质量标准初步研究

目的初步建立母鸡油制三七饮片的质量标准。方法采用HPLC测定饮片中人参皂苷Rg1、人参皂苷Rb1及三七皂苷R1的含量;对10批次三七生品及制品的水分、总灰分、酸不溶性灰分、醇溶性浸出物、Rg1、Rb1和R1进行含量测定,并进行分析评价。结果建议母鸡油制三七饮片水分不超过3.9%,总灰分不超过3.0%,酸不溶性灰分不超过0.9%,以干品计醇溶性浸出物不得少于14.2%,人参皂苷Rg1、人参皂苷Rb1及三七皂苷R1的总含量不得少于3.4%。结论初步建立母鸡油制三七饮片量化质控标准评价体系。     

云南省不同产地三七的成分分析

目的对云南省不同产地三七的成分进行分析。方法采用L9(34)正交实验,优化三七中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的超声提取方法。利用HPLC法测定云南省不同产地的21批三七样品中三种皂苷的含量,SPSS数据分析软件比较分析其含量数据差异。结果云南省不同产地的21批三七样品中3种皂苷的含量范围分别是:三七皂苷R10.30～1.43 mg/g、人参皂苷Rg11.77～5.52 mg/g、人参皂苷Rb11.76～3.44 mg/g。21批云南不同产地三七样品中皂苷含量均符合药典要求,其中采自大理云龙的三七样品中各皂苷含量均较高,甚至高于来自道地产区文山的样品。同时建立三七的HPLC指纹图谱,样品出峰个数为32个,选取人参皂苷Rb1为参照峰,共有峰占总峰数比例达到71.88%。结论云南省不同产地的三七样品所含成分基本一致。方法简便、灵敏,可用于不同产地三七的成分质量分析及评价。     

不同伴生物三七总皂苷指纹图谱研究

目的建立不同伴生物三七总皂苷的指纹图谱,对其化学成分进行综合评价。方法对4种不同伴生物三七总皂苷,各6批,采用HPLC法进行分析,制定以三七皂苷R1、人参皂苷Rg1及人参皂苷Rb1为参照物的指纹图谱;运用"中药色谱指纹图谱相似度评价系统(2004A)版",计算各被测样品的HPLC指纹图谱的整体相似度。结果不同伴生物三七总皂苷各有10个共有峰、6批被测样品的色谱指纹图谱的整体相似度均在0.99以上,并得出了各自相应的对照指纹图谱。结论该指纹图谱方法简便、重现性好,表明了不同伴生物三七总皂苷成分分布及比例稳定、均匀一性良好,制备方法稳定、合理、可靠。     

朝鲜红参中人参皂苷类成分含量测定及指纹图谱研究

目的:建立可同时测定朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg15种成分含量和指纹图谱的HPLC方法。方法:采用DiscoveryC18(4.6×250mm,5μm)柱,流动相为水(A)-乙腈(B)梯度洗脱;流速1.0ml min-1,柱温为25℃,检测波长为203nm,并用计算机辅助相似性评价系统对指纹图谱进行了相似度分析。结果:朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg1的线性范围分别为1.077~5.385μg,1.046~5.230μg,0.0953~0.4765μg,1.018~5.090μg,0.973~4.865μg,相关系数分别为0.9999,0.9999,0.9995,0.9995,0.9996。人参皂苷Rb1、Rb2、Rb3、Re、Rg1平均回收率分别为96.55%、101.4%、99.08%、99.92%、96.00%;RSD分别为1.6%、1.2%、1.5%、1.2%、1.3%。不同规格的朝鲜红参之间及朝鲜红参与国产红参都具有较好的相关性。结论:该方法重复性好,可以用来同时测定朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg1五种成分的含量,14批不同规格的朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re和Rg15种成分含量存在差异,但总体而言,朝鲜红参与国产红参在人参皂苷含量和指纹图谱上无明显差异。     

HPLC同时测定乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量

目的 建立高效液相色谱法测定乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量。 方法 色谱柱为CAPCELL PAK C₁₈(150 mm×4.6 mm,5 μm);流动相A为乙腈,B为水;流速：1.0 mL·min^-1;检测波长为203 nm;洗脱程序：0~12 min,A相19%,B相81%;12~60 min,A为19%→36%,B为81%→64%。结果 三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1保留时间分别为21,25,51 min,与各自相邻峰的分离度均在1.5以上。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为0.100 2~2.00 4 μg、0.418 8~8.376 μg和0.187~3.74 μg。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的回收率分别为98.5%,99.6%和96.5%,RSD分别为1.9%,1.3%和1.9%。结论 本法简便、准确、重现性好,可用于乳癖消颗粒中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1 含量的同时测定。     

HPLC法测定三七总皂苷分散片中三种皂苷的含量

目的:采用HPLC法测定三七总皂苷分散片中三七皂苷R1、人参皂苷Rb1和Rg1三种皂苷含量,为建立三七总皂苷分散片质量标准含量测定方法提供依据。方法:用Zorbax Eclipse XDB-C18(4.6×250mm,5μm)分析柱;乙腈-水线性梯度洗脱;流速1.0mL/m in,检测波长203nm。结果:三七皂苷R1、人参皂苷Rg1和Rb1的线性范围分别为0.12~2.4μg,0.4~8μg,0.5~10μg。该方法回收率三七皂苷R1为99.36%(RSD=1.53%)、人参皂苷Rg1为98.69%(RSD=0.86%)和人参皂苷Rb1为98.92%(RSD=1.27%)。结论:HPLC梯度洗脱能将三种皂苷很好地分离检测,方法准确灵敏,稳定可靠,可用于三七总皂苷分散片的质量控制。     

丹参破壁饮片的质量研究

目的:对丹参破壁饮片质量进行研究,为丹参破壁饮片质量标准的建立提供实验依据。方法:采用BT-9300HT型激光粒度分析仪测定丹参破壁饮片的粒径分布;采用薄层色谱法进行定性鉴别;采用高效液相色谱法测定其丹参酮、丹酚酸的含量,并结合"中药色谱指纹图谱相似度评价系统2012版"软件对各批次丹参破壁饮片指纹图谱进行分析。结果:十批丹参破壁饮片粒径分布范围在30.38～63.44μm之间;薄层鉴别中,各批次样品所显主斑点的颜色和位置与对照药材一致;各批次丹参破壁饮片中三种丹参酮总含量为1.0%～3.5%,丹酚酸B含量为3.0%～4.5%;各批次丹参破壁饮片的指纹图谱考察相似度在0.967～1.000之间。结论:本文所建立的方法稳定、可靠、重复性好,可用于丹参破壁饮片的质量控制和评价。     

UPLC测定三七总皂苷微丸中5种皂苷类成分含量研究

目的经建立超高效液相色谱法(UPLC)法测定三七总皂苷微丸中三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、人参皂苷Rd 5种皂苷类成分含量的方法,并测定6批样品的各成分含量。方法采用ACQUITY UPLC~?BEH C-18(2.1 mm×50 mm,1.7μm)色谱柱,以乙腈(C)-水(D)为流动相梯度洗脱(0～1.7 min,20%C;1.7～4.83 min,20%C～46%C;4.83～6.08 min,46%C～33%C;6.08～6.70 min,33%C～20%C;6.70～11.00 min,20%C),检测波长为203 nm,流速为0.5 mL·min~(-1),柱温为25℃。结果三七总皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、人参皂苷Rd分别在67.74～237.09、250.44～876.54、257.28～900.48、202.70～709.46、6.96～41.76 mg/mL范围内线性关系良好(r≥0.999),平均加样回收率分别为105.2%、104.33%、94.63%、93.65%、106.52%;6批三七总皂苷微丸各成分含量均值分别为1.55%、6.55%、0.67%、6.02%、1.40%。结论建立的含量测定方法准确简便、重复性好,6批三七微丸样品含量接近,工艺稳定。     

三七总皂苷凝胶膏剂质量标准研究

目的：建立三七总皂苷凝胶膏剂的质量标准.方法：采用薄层色谱法进行定性鉴别;高效液相色谱法对三七总皂苷凝胶膏剂中的三七皂苷Rl、人参皂苷Rg1、人参皂苷Rb1三种成分进行测定;按《中国药典》（2010年版一部）方法考察凝胶膏剂的含膏量及赋形性.结果：三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1在同一块薄层板上分离度良好,专属性强;三七总皂苷凝胶膏剂中三种成分的含量稳定,其含量分别为三七皂苷R11.68mg·g-1,人参皂苷Rg114.6mg·g-1,人参皂苷Rb13.30mg·g-1;其含膏量及赋形性符合要求.结论：该方法可靠、简便、准确,可作为三七总皂苷凝胶膏剂的质量控制方法.     

HPLC法测定三七止血胶囊中三七总皂苷含量研究

目的:完善三七止血胶囊的质量标准,控制药品质量。方法:采用高效液相色谱法测定三七止血胶囊中三七皂苷R1﹑人参皂苷Rg1﹑人参皂苷Rb1的含量。色谱柱:色谱柱:C18Alltima 250×4.6 mm,5μL;流动相:以流动相A:乙腈,流动相B:水,采用梯度洗脱方式测定,检测波长:203nm;流速:1.0mL/min。结果:测定10批样品,三七皂苷的平均含量为3.04 mg/粒,三七皂苷R1线性范围为0.1008～0.9072μg,相关线性系数:R=0.9999,人参皂苷Rg1线性范围为0.4160～3.7440μg,相关线性系数:R=0.9999,人参皂苷Rb1线性范围为0.4136～3.7224μg。相关线性系数:R=0.9999,平均回收率:94.41%,RSD:0.74%(n=5)。结论:建立的方法简便易行,重现性好,可有效的控制该制剂的质量。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.