Basaloid squamous carcinoma of the esophagus:clinicopathological,immunohistochemical and electron microscopic studies of 16 cases

Ｂａｓａｌｏｉｄｓｑｕａｍｏｕｓｃａｒｃｉｎｏｍａｏｆｔｈｅｅｓｏｐｈａｇｕｓ：ｃｌｉｎｉｃｏｐａｔｈｏｌｏｇｉｃａｌ，ｉｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｃａｌａｎｄｅｌｅｃｔｒｏｎｍｉｃｒｏｓｃｏｐｉｃｓｔｕｄｉｅｓｏｆ１６ｃａｓｅｓＺｈａｎｇＸｉｎ...     

光镜和电镜下肠癌与血吸虫病肠的改变及其关系的探讨

The light and electron microscopic observations were carried out in 50 cases of colon specimens. No remarkable morphologic differences were found between the epithelial cells of the mucosa with schistosomiasis, colitis and the normal specimens. Cells of the colon carcinoma also showed no significant difference from those of the colon carcinoma associated with schistosomiasis. Atypical changes in the epithelial cells were not seen in the areas both adjacent to and remote from the carcinoma and in the egg deposit areas. Under LM, changes were manifested in various degrees in degeneration and disassociation of mucosal cells in colitis (1), schistosomiasis (1) and carcinoma associated with schistosomiasis (8). Under EM, changes in mucosal muscle cells were seen in colon carcinoma (2) and carcinoma associated with schistosomiasis (5). Proliferation of fibroblasts and hyperchromasia of the nuclei were noted in 3 cases of carcinoma associated with schistosomiasis. The pathological changes mentioned above were more prominent in cases of carcinoma associated with schistosomiasis than in those of colon carcinoma alone. The authors believed that the cause was non-specific changes induced by the toxin of the egg mechanical effects rather than a direct carcinogenic action of the eggs, and that the non-specific changes implied no direct relationship between the development of colon carcinoma and schistosomiasis.     

Orbital rhabdomyosarcoma. Immunohistochemical studies of seven cases.

An immunohistochemical analysis using a series of mono- and polyclonal antibodies against myoglobin, desmin, vimentin, actin, NSE, S-100 protein and keratin was done on alveolar rhabdomyosarcoma (3 cases), embryonal (3) and pleomorphic rhabdomyosarcoma of the orbit (1). All tumors were stained by monoclonal antibodies to desmin and vimentin, whereas 6 of the 7 cases were stained by myoglobin. The results indicate that desmin and vimentin are highly sensitive immunohistochemical markers for the diagnosis of rhabdomyosarcoma, especially in poorly differentiated ones.

     

45例原发性肺癌超微结构观察

用透射电镜观察了45例原发性肺癌超微结构。结果表明:经光镜诊断的各型肺癌在电镜下可以表现为不同的其他类型:大细胞癌可表现为腺癌、鳞癌、腺鳞癌和未分化癌的超微结构特征。各型肺癌细胞浆内均可见板层小体。细胞内微腔由细胞膜反折形成。腺鳞癌在电镜下比例升高,可能与绝大多数肺癌起自呼吸道上皮的基底细胞及粘液细胞有关。     

Congenital epulis--an electron microscopic observations of two cases

Two cases of congenital epulis occurring in the gingiva at the site where the lower deciduous incisors were to erupt are described. The first patient was a 7 day-old female infant. Her epulis was diagnosed, histopathologically, as a granular cell tumor. Electron microscopy revealed that the tumor cells were large with small eccentric nuclei. The cells were filled with granular structures. These granular substances varied in size and electron density. Case 2 involved a 37 day-old male infant. A histopathological diagnosis of fibromatosis was made. Electron micrographs showed a proliferation of cells which resembled fibroblasts. These cells varied in shape, including some with thin, long processes. Abundant reticular and collagen fibers were present in the stroma. In both cases, the postoperative course was uneventful without recurrence.     

两例KAWASAKI综合征皮疹光镜和电镜病理学观察

本文对临床诊断为 Kawasaki 综合征两例的皮疹活检做了光镜和电镜观察,结果发现真皮乳头层明显水肿,皮肤毛细血管受损,管周有明显的单核细胞浸润,纤维结缔组织发生明显肿胀和变性,于其中一例的超薄切片中,电镜下发现一种特殊的螺旋状小体,这一发现为今后进行病因学研究,提供一个线索。     

甲状腺癌100例术后放射治疗

分析甲状腺癌术后放射治疗疗效。方法１９７８年６月至１９８６年６月收治的甲状腺术后患者１００例，年龄７－６５岁，平均年龄３５岁。术后估计切除干净行预防性放疗２８例，术后残余癌行放疗７２例。所有病例均有术后病理诊断。全组均采用＾６０Ｃｏ－γ线外照射部分患者加用深度Ｘ线照射；术后行预防性放疗者，照射野包括原病变局部和瘤床区。对分化程度低，有淋巴结围歼者，设野靶区自扩大包括双颈，病变达胸骨后上份者包括上纵     

人原发性肝细胞癌中HBxAg的免疫组化及免疫电镜研究

作者用免疫组化和胶体金免疫电镜方法,观察乙型肝炎x抗原(HBxAg)在肝癌组织(35例)及癌旁肝组织(29例)内的分布及亚细胞定位。结果显示,HBxAg在癌组织及癌旁肝组织内的检出率分别为60.0％和82.8％,阳性物质主要位于细胞浆内。电镜观察,HBxAg主要定位于粗面内质网和胞浆基质中,少数位于核内,并可见游离核糖体及核孔附近阳性,推测HBxAg在内质网及游离核糖体上合成,并可经核孔进入核内。     

丙型肝炎患者外周血单个核细胞HCV免疫组化和电镜研究

目的：探讨丙型肝炎患者外周血单个核细胞（PBMCs）内丙型肝炎病毒抗原（HCVAg）表达和HCV颗粒感染状况及意义。方法：采用抗HCV核心区及NS3区单克隆抗体，对35例慢性丙肝患者PBMCs进行免疫组化检测和电镜观察。结果：慢性丙肝患者PBMCs的HCVAg阳性率为82.9%(29/35），其中82.8%（24/29）的病例电镜下发现了病毒颗粒，占全组病例的68.6%(24/35)。同时发现部分细胞超微结构呈现一系列凋亡性改变。结论：HCV可感染PBMCs并在其中复制，这可能是导致患者HCV持续感染的原因之一。     

60例胃癌临床资料分析

目的：探讨胃癌的临床特点以及内镜、CT对胃癌的术前诊断价值。方法：对60例手术切除，且具备内镜、CT检查资料的进展期胃癌病例进行回顾性分析。结果：胃癌的临床表现缺乏特异性；内镜是直接、准确的检查方法，对肿瘤腔内的诊断准确率达95.0%；CT能估计肿瘤的腔外扩展情况。结论：重视非特异性临床表现，综合内镜、CT检查能提高对胃癌的术前诊断水平，从而使胃癌得到及早诊断和合理治疗。     

Subtypes of intestinal metaplasia and gastric carcinoma. A clinicoendoscopic follow-up of 112 cases.

Nine tumor-associated antigens (MG7-, MGdl-, MC3-corresponding antigens, CEA, P21ras, Sialoglycoprotein, CA 19-9-like, Sialo-Tn and Le8-like antigens) were investigated on biopsy specimens of different types of intestinal metaplasia (types I, II and III) taken from 112 patients with benign gastric conditions. The incidence of positive staining for 9 antigens except for P21ras in type III intestinal metaplasia was significantly higher than that in types I and II (P less than 0.05-0.01). These 112 patients were clinicoendoscopically followed up for 15-70 months. Five of them were found to develop gastric carcinomas within 25-60 months, giving a cancer detection rate of 4.5%. They were detected from type III (16.1%) patients, but none was found in patients of types I and II, and the differences were significant (P less than 0.05-0.01). Our results indicate that type III intestinal metaplasia has a higher potentiality in developing malignancy and that MG7, MGd1, MC3, CEA, CA19-9-like and Sialo-Tn antigens are valuable tumor markers in defining the high-risk group of gastric carcinoma.