丹参注射液对2型糖尿病下肢血管病变患者炎症学指标的影响

目的检测2型糖尿病下肢血管病变的炎症学指标变化,并观察丹参注射液对炎症因子的干预治疗效果。方法将90例2型糖尿病下肢血管病变患者分为两组,治疗组在常规治疗基础上加用丹参注射液,对照组给予常规治疗。观察两组治疗前后血清炎症因子c反应蛋白（CRP）、白细胞介素6（IL-6）水平的变化,并与2型糖尿病无下肢血管病变患者进行对比。结果糖尿病无下肢血管病变患者血清CRP、IL-6水平明显高于糖尿病无下肢血管病变患者,治疗组治疗后血清CRP、IL-6水平明显低于对照组（P〈0．05或〈0．01）。结论2型糖尿病下肢血管病变患者炎症学指标明显升高．丹参注射满对炎瘫因子的干预治疗效果佩著。     

2型糖尿病下肢血管病变与DNA氧化损伤的关系

目的探讨2型糖尿病合并下肢血管病变与机体DNA氧化损伤的关系。方法纳入门诊85例糖化血红蛋白9%的2型糖尿病病人作为研究组,按照踝肱比(ABI)水平将上述病人分为糖尿病合并下肢血管病变组(n=35)及无并发症组(n=50);同时取60例正常人群作为正常对照组。使用ELISA方法测定血液单核细胞DNA氧化损伤指标8羟基脱氧鸟嘌呤(8-OHdG)水平,并在3组间进行比较,同时采用Logistic回归分析糖尿病合并下肢血管病变的危险因素。结果糖尿病合并下肢血管病变组与无并发症组间年龄、病程、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白等指标差异无统计学意义(P0.05)。糖尿病合并下肢血管病变组血液8-OHd G水平为(4.03±1.89)ng/ml,无并发症组血液8-OHd G水平为(2.74±1.64)ng/ml,均较正常对照组[(0.53±0.53)ng/ml]升高(P0.05),且糖尿病合并下肢血管病变组较无并发症组亦升高(P0.05)。Logistic回归分析显示,8-OHd G以及LDL-C是糖尿病合并下肢血管病变的危险因素。结论 2型糖尿病合并下肢血管病变病人体内DNA氧化损伤较正常人及无并发症糖尿病病人严重,提示DNA氧化损伤在糖尿病下肢血管病变中发挥了一定程度的致病作用。     

C反应蛋白与2型糖尿病大血管病变危险因素的相关性研究

2型糖尿病(DM)合并大血管病变者血清C反应蛋白(CRP)水平明显高于2型DM无大血管病变组及正常对照组(P<0.01),2型DM无大血管病变组高于正常对照组(P<0.01)。提示CRP可能是2型DM和2型DM大血管病变的危险因子,炎症可能参与了2型DM及2型DM大血管病变的发生和发展。     

彩超诊断2型糖尿病合并下肢血管病变的临床意义

目的探析彩超诊断2型糖尿病合并下肢血管病变的临床意义。方法以该院2013年10月—2014年5月间收治的30例2型糖尿病合并下肢血管病变患者为研究对象,设为观察组,另以同时期的30例体检健康者为参照对象,设为对照组。两组均行彩超检查,比较两组的下肢动脉血管病变的检查结果。结果观察组血管粥样硬化斑块、血管狭窄、血管闭塞检出率均高于对照组,而观察组踝肱指数低于对照组,且0.8,两组差异有统计学意义(P0.05)。结论 2型糖尿病患者的下肢血管病变彩检出率较高,彩超能帮助临床了解2型糖尿病患者下肢血管病变患者的病变情况,对诊断有重要意义;彩超检查还有助于了解患者的血管病变情况,提高了治疗的针对性。     

前列地尔治疗糖尿病下肢血管病变的效果分析

目的探讨采用前列地尔对糖尿病下肢血管病变患者完成治疗后获得的临床效果。方法选择该院2014年1月—2015年12月收治的糖尿病下肢血管病变患者40例作为该次实验对比观察对象;观察组20例以及对照组20例患者的分组依据为糖尿病下肢血管病变治疗方法的不同;对照组:丹参川芎嗪注射液;观察组:前列地尔注射液+丹参川芎嗪注射液;通过对比症状缓解情况、下肢踝肱指数以及足背动脉血流变化,以突出前列地尔的临床应用价值。结果在症状缓解情况方面,观察组优于对照组糖尿病下肢血管病变患者明显(P0.05);在下肢踝肱指数方面,观察组优于对照组糖尿病下肢血管病变患者明显(P0.05);在足背动脉血流变化方面,观察组优于对照组糖尿病下肢血管病变患者明显(P0.05)。结论对于糖尿病下肢血管病变患者,治疗药物选择前列地尔,可以成功改善患者的下肢踝肱指数以及患者的足背动脉血流变化,最终显著提高糖尿病下肢血管病变患者的生活质量。     

2型糖尿病炎症因子水平与心脑血管病变的相关性

目的 探讨2型糖尿病合并冠心病或脑梗死患者炎性因子水平的变化及其在心脑血管病变中的作用.方法 选取49例2型糖尿病未合并大血管病变的患者纳入对照组,20例2型糖尿病合并大血管病变患者纳入观察组,选取同龄正常人群为正常组,比较三组人群的炎症因子水平以及颈动脉病变程度,分析其对糖尿病性大血管病变的影响.结果 观察组收缩压、FBG、TG升高最为明显,且合并颈动脉内膜增厚、粥样斑块形成及颈动脉狭窄比例最高.2型糖尿病患者sICAM-1、sVCAM-1、MCP-1及TNF-α均明显高于正常组(P＜0.05),而观察组上述指标与对照组也存在显著性差异(P＜0.05).结论 炎症因子在2型糖尿病合并心脑血管病变的患者中升高,在心脑血管病变中具有重要作用.     

丹参片对心力衰竭合并2型糖尿病病人炎症因子及预后的影响

目的评价丹参片对心力衰竭合并2型糖尿病病人肿瘤坏死因子-α(TNF-α)、高敏C-反应蛋白(hs-CRP)、6min步行距离(6-MWT)等影响及其长期临床疗效。方法入选100例并发2型糖尿病的心力衰竭病人,随机分为丹参组和常规组,各50例。丹参组在常规组治疗基础上加用丹参片(每日3次,每次3片),随访1年。观察治疗前后心功能、6-MWT、超声心动图指标、住院率和病死率以及血清TNF-α、hs-CRP水平。结果两组治疗1年后心功能、6-MWT、超声心动图指标皆明显改善,TNF-α、hs-CRP水平明显下降。与常规组比较,丹参组心功能、6-MWT提高更明显,TNF-α、hs-CRP水平下降更显著。而且丹参组因心血管事件住院率也显著低于常规组。结论在常规西药治疗基础上加用丹参片能抑制并发2型糖尿病的心力衰竭病人炎症反应,改善心功能,提高生活质量,改善预后。     

血小板参数、TNF-α与糖尿病下肢血管病变的关系

目的针对肿瘤坏死因子-α(TNF-α)、血小板参数和2型糖尿病下肢血管病变之间的关系进行分析与研究。方法 2018年1—6月间对30名对照组中的健康者以及60例2型糖尿病患者的TNF-α与血小板参数进行检测。结果下肢血管病变组的血小板分布宽度(PDW)、血小板压积(PCT)、TNF-α比对照组与单纯糖尿病组更高,从血小板计数(PLT)上看,下肢血管病变组要比对照组与单纯糖尿病组更低,,差异有统计学意义(P0.05);从PLT上看,单纯糖尿病组比对照组低,同时,从PDW、PCT、TNF-α上看,单纯糖尿病组比对照组高,但差异无统计学意义(P0.05)。结论糖尿病下肢血管病变和TNF-α、血小板参数存在密切的相关性,在临床上应加大力度关注早期监测在诊断2型糖尿病下肢血管病变的重要意义。     

他汀序贯疗法对急性冠状动脉综合征合并2型糖尿病患者炎性因子和脂联素影响的研究

目的探讨他汀序贯疗法对急性冠状动脉综合征(ACS)合并2型糖尿病患者炎性因子和脂联素的影响。方法选择ACS患者119例,将合并糖尿病患者61例分为对照1组32例和序贯治疗1组29例,ACS未合并糖尿病患者58例分为对照2组28例和序贯治疗2组30例;另选糖尿病患者31例为糖尿病对照组,正常体检者26例为正常对照组。比较各组脂联素、可溶性血管细胞黏附因子1(sVCAM-1)、脂蛋白磷脂酶A2(Lp-PLA2)、妊娠相关蛋白A(PAPP-A)水平。结果与正常对照组比较,糖尿病对照组脂联素明显降低,sVCAM-1、Lp-PLA2、PAPP-A明显升高(P<0.01)。与对照1组和对照2组比较,序贯治疗1组和序贯治疗2组脂联素水平明显升高,sVCAM-1、Lp-PLA2、PAPP-A明显降低(P<0.01)。结论他汀序贯疗法可明显提高合并或未合并糖尿病ACS患者的脂联素水平,降低其炎症标记物水平。ACS合并或未合并糖尿病患者应用他汀序贯疗法后,对脂联素和血清炎症标记物的影响无明显差异。     

2型糖尿病下肢血管病变的相关因素的分析

<正>2型糖尿病合并下肢血管病变是导致下肢的主要原因[1]。本研究应用彩色普勒超声对糖尿病下肢血管病变的发生程度进行检测,并分析其可能的相关危险因素。1资料与方法收集糖尿病病人160例。其中90例为合并下肢血管病变(A组),年龄39～68岁,病程3月～11年,70例为单纯糖尿病者(B组),年龄22～60岁,病程1月～5年。     

妊娠时间与肺结核复发的相关性研究及诊治对策

目的 分析肺结核史患者妊娠时间和肺结核复发间相关性.方法 选取我院收治的有肺结核史的妊娠妇女576例作为研究对象,对其妊娠前肺结核治疗、治愈后妊娠时间、妊娠后复发肺结核等进行分析,总结有肺结核史育龄女性的妊娠时间和肺结核复发之间的关系.结果 肺结核治愈后不同时间段妊娠者的结核复发率比较,差异具有显著性（P＜0.05）,停药后间隔时间越久妊娠,肺结核复发的几率越小.结论 加强孕期痰菌检查,及早发现复发肺结核,提高母婴安全.     

我国骨关节结核诊治的回顾与展望

骨关节结核是危害人们健康的严重感染性疾病,近95％由他处结核病继发而来.罹患骨关节结核疾病后几乎均将致残,严重影响人们的健康、工作和生活.建国以来在党和国家的关心和支持下,骨关节结核的诊治水平取得了长足进步.时至今日,由于多种原因,学科发展和被重视程度受到一定的制约,同整个医疗行业的发展不相适应.回顾过去,展望未来,我们需要重新审视骨关节结核的诊治方法,努力推进骨关节结核诊疗技术的科学发展.     

Effect of phosphorylation of MAPK and Stat3 and expression of c-fos and c-jun proteins on hepatocarcinogenesis and their clinical significance

AIM To study the effect of phosphorylation ofMAPK and Stat3 and the expression of c-fos andc-jun proteins on hepatocellular carcinogenesisand their clinical significance.METHODS SP immunohistochemistry was usedto detect the expression of p42/44~(MAPK), p-Stat3,c-fos and c-jun proteins in 55 hepatocellularcarcinomas (HCC) and their surrounding livertissues.RESULTS The positive rates and expressionlevels of p42/44~(MAPK), p-Stat3, c-fos and c-junproteins in HCCs were significantly higher thanthose in pericarcinomatous liver tissues (PCLT).A positive correlation was observed between theexpression of p42/44~(MAPK) and c-fos proteins, andbetween p-Stat3 and c-jun, but there was nosignificant correlation between P42/44~(MAPK) and p-Stat3 in HCCs and their surrounding livertissues.CONCLUSION The abnormalities of Ras/Raf/MAPK and JAKs/ Stat3 cascade reaction maycontribute to malignant transformation ofhepatocytes. Hepatocytes which are positive forp42/ 44~(MAPK), c-fos or c-jun proteins may bepotential malignant pre-cancerous cells.Activation of MAPK and Stat3 proteins may be anearly event in hepatocellular carcinogenesis.     

Overweight and Obesity and Progression of ADPKD

Open in a separate window     

Effects of sex and time of day on metabolism and excretion of corticosterone in urine and feces of mice

Non-invasive techniques to monitor stress hormones in small animals like mice offer several advantages and are highly demanded in laboratory as well as in field research. Since knowledge about the species-specific metabolism and excretion of glucocorticoids is essential to develop such a technique, we conducted radiometabolism studies in mice (Mus musculus f. domesticus, strain C57BL/6J). Each mouse was injected intraperitoneally with 740 kBq of 3H-labelled corticosterone and all voided urine and fecal samples were collected for five days. In a first experiment 16 animals (eight of each sex) received the injection at 9 a.m., while eight mice (four of each sex) were injected at 9 p.m. in a second experiment. In both experiments radioactive metabolites were recovered predominantly in the feces, although males excreted significantly higher proportions via the feces (about 73%) than females (about 53%). Peak radioactivity in the urine was detected within about 2h after injection, while in the feces peak concentrations were observed later (depending on the time of injection: about 10h postinjection in experiment 1 and about 4h postinjection in experiment 2, thus proving an effect of the time of day). The number and relative abundance of fecal [3H]corticosterone metabolites was determined by high performance liquid chromatography (HPLC). The HPLC separations revealed that corticosterone was extensively metabolized mainly to more polar substances. Regarding the types of metabolites formed, significant differences were found between males and females, but not between the experiments. Additionally, the immunoreactivity of these metabolites was assessed by screening the HPLC fractions with four enzyme immunoassays (EIA). However, only a newly established EIA for 5alpha-pregnane-3beta,11beta,21-triol-20-one (measuring corticosterone metabolites with a 5alpha-3beta,11beta-diol structure) detected several peaks of radioactive metabolites with high intensity in both sexes, while the other EIAs showed only minor immunoreactivity. Thus, our study for the first time provides substantial information about metabolism and excretion of corticosterone in urine and feces of mice and is the first demonstrating a significant impact of the animals' sex and the time of day. Based on these data it should be possible to monitor adrenocortical activity non-invasively in this species by measuring fecal corticosterone metabolites with the newly developed EIA. Since mice are extensively used in research world-wide, this could open new perspectives in various fields from ecology to behavioral endocrinology.     

Effect of phosphorylation of MAPK and Stat3 and expression of c-fas and c-jun proteins on hepatocarcinogenesis and their clinical significance

AIM To study the effect of phosphorylation ofMAPK and Stat3 and the expression of c-fos andc-jun proteins on hepatocellular carcinogenesisand their clinical significance.METHODS SP immunohistochemistry was usedto detect the expression of p42/44MAPK, p-Stat3,c-fos and c-jun proteins in 55 hepatocellularcarcinomas (HCC) and their surrounding livertissues.RESULTS The positive rates and expressionlevels of p42/44MAPK, p-Stat3, c-fos and c-junproteins in HCCs were significantly higher thanthose in pericarcinomatous liver tissues (PCLT).A positive correlation was observed between theexpression of p42/44MAPK and c-fos proteins, andbetween p-Stat3 and c-jun, but there was nosignificant correlation between p42/44MAPK and p-Stat3 in HCCs and their surrounding livertissues.CONCLUSION The abnormalities of Ras/Rat/MAPK and JAKs/ Stat3 cascade reaction maycontribute to malignant transformation ofhepatocytes. Hepatocytes which are positive forp42/ 44MAPK, c-fos or c-jun proteins may bepotential malignant pre-cancerous cells.Activation of MAPK and Stat3 proteins may be anearly event in hepatocellular carcinogenesis.     

Replication and Inhibitors of Enteroviruses and Parechoviruses

The Enterovirus (EV) and Parechovirus genera of the picornavirus family include many important human pathogens, including poliovirus, rhinovirus, EV-A71, EV-D68, and human parechoviruses (HPeV). They cause a wide variety of diseases, ranging from a simple common cold to life-threatening diseases such as encephalitis and myocarditis. At the moment, no antiviral therapy is available against these viruses and it is not feasible to develop vaccines against all EVs and HPeVs due to the great number of serotypes. Therefore, a lot of effort is being invested in the development of antiviral drugs. Both viral proteins and host proteins essential for virus replication can be used as targets for virus inhibitors. As such, a good understanding of the complex process of virus replication is pivotal in the design of antiviral strategies goes hand in hand with a good understanding of the complex process of virus replication. In this review, we will give an overview of the current state of knowledge of EV and HPeV replication and how this can be inhibited by small-molecule inhibitors.     

心电图、心电向量图与冠状动脉造影结果对比分析

目的:通过分析心电图(Electrocardiogram,ECG)和心电向量图(Vectorcardiogram,VCG)的改变与冠脉造影（CAG）结果进行对比,探讨ECG、VCG在冠状动脉病变中的诊断价值。方法: 选择2008年1月~2009年12月临床拟诊断为冠心病患者108例,行常规ECG、VCG检查,并于1周内进行CAG,对检查结果依据各自的诊断标准进行判定,以CAG为标准诊断法,利用四格表法,计算相关评价真实性的指标并进行比较。结果: ①VCG检测的灵敏度、特异度、准确度显著高于ECG(P<0.05,P<0．01)。②ECG、VCG阳性率与冠脉病变支数组间比较:在单支病变、双支病变中,VCG阳性率明显高于ECG(P<0.05),左主干或三支病变无统计学意义;组内比较:ECG组左主干或三支病变组较单支病变、双支病变阳性率高(P<0.05,P<0.01);VCG组左主干或三支病变组较单支病变阳性率高(P<0.05);与双支病变阳性率比较无统计学意义;③ECG、VCG阳性率与冠脉病变程度组间比较:冠脉病变狭窄50%～69%的VCG阳性率明显高于ECG (P<0.05),其他两组阳性率比较无统计学意义;组内比较:ECG组冠脉病变狭窄≥90%较50%～69%、70%～89%的阳性率高(P<0.05,P<0.01); VCG组狭窄≥90%较50%～69%阳性率高（P<0.01),其他无统计学意义。结论: VCG对冠心病检测价值显著高于ECG。     

Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence

Here we report the structural characterization of the product formed from the reaction between hydroethidine (HE) and superoxide (O(2)(.-)). By using mass spectral and NMR techniques, the chemical structure of this product was determined as 2-hydroxyethidium (2-OH-E(+)). By using an authentic standard, we developed an HPLC approach to detect and quantitate the reaction product of HE and O(2)(.-) formed in bovine aortic endothelial cells after treatment with menadione or antimycin A to induce intracellular reactive oxygen species. Concomitantly, we used a spin trap, 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BMPO), to detect and identify the structure of reactive oxygen species formed. BMPO trapped the O(2)(.-) that formed extracellularly and was detected as the BMPO-OH adduct during use of the EPR technique. BMPO, being cell-permeable, inhibited the intracellular formation of 2-OH-E(+). However, the intracellular BMPO spin adduct was not detected. The definitive characterization of the reaction product of O(2)(.-) with HE described here forms the basis of an unambiguous assay for intracellular detection and quantitation of O(2)(.-). Analysis of the fluorescence characteristics of ethidium (E(+)) and 2-OH-E(+) strongly suggests that the currently available fluorescence methodology is not suitable for quantitating intracellular O(2)(.-). We conclude that the HPLC/fluorescence assay using HE as a probe is more suitable [corrected] for detecting intracellular O(2)(.-).     

大鼠骨髓间充质干细胞的分离培养和外源基因的导入

目的探讨绿色荧光蛋白基因转染骨髓间质干细胞的可行性。方法采用F icoll-PaqueTMP lus淋巴细胞分离液,根据细胞密度梯度原理,分离大鼠骨髓间充质干细胞(rM SC s)并进行体外原代培养和传代扩增,倒置相差显微镜观察细胞生长情况,免疫细胞化学法对其初步鉴定。流式细胞仪分析转染效率。结果原代和传代培养的细胞呈现梭形外观,具有较强的生长增殖能力;细胞均一表达CD44、CD54、CD106、CD29抗原。电穿孔法转染rM SC s转染率为32.8%±3%。结论采用比重为1.077 g/L的F icoll-PaqueTMP lus能分离获得大鼠骨髓间充质干细胞,经原代培养和传代培养能够迅速扩增。电穿孔法具有较高的介导外源基因表达于rM SC s的效率。