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Taku Wakahara Ryoichi Ema Naokazu Miyamoto Yasuo Kawakami 《Clinical physiology and functional imaging》2017,37(4):405-412
The purpose of this study was to examine whether inter‐ and intramuscular differences in hypertrophy induced by resistance training correspond to differences in muscle activation during the first training session. Eleven young men completed 12 weeks of training intervention for knee extension. Before and after the intervention, T1‐weighted magnetic resonance (MR) images were recorded to determine the volume and anatomical cross‐sectional area (CSA) along the length of the individual muscles of the quadriceps femoris. The T2‐weighted MR images were also acquired before and immediately after the first training session. The T2 was calculated for each pixel within the quadriceps femoris, from which the muscle activation was evaluated as %activated volume and area. The results showed that the %activated volume after the first training session was significantly higher in the vastus intermedius than the vastus medialis. However, the relative change in muscle volume after the training intervention was significantly greater in the rectus femoris than the vasti muscles (vastus lateralis, intermedius and medialis). Within the rectus femoris, both the %activated area and relative increase in CSA were significantly greater in the distal region than the proximal region. In contrast, the %activated area and relative increase in CSA of the vasti were nearly uniform along each muscle. These results suggest that the muscle activation during the first training session is associated with the intramuscular difference in hypertrophy induced by training intervention, but not with the intermuscular difference. 相似文献
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Measuring Outcomes: Pain and Quality of Life 48 Months After Acute Postoperative Total Knee Prosthetic Joint Infection 下载免费PDF全文
Montserrat Núñez PhD Felix Vilchez Cavazos PhD Esther Núñez Juarez PhD Juan Carlos Martinez‐Pastor MD Francisco Maculé Beneyto PhD Santiago Suso PhD Alex Soriano Viladomiu PhD 《Pain practice》2015,15(7):610-617
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Background
KRAS mutations are the key indicator for EGFR monoclonal antibody‐targeted therapy and acquired drug resistance, and their accurate detection is critical to the clinical decision‐making of colorectal cancer. However, no proper quality control material is available for the current detection methods, particularly next‐generation sequencing (NGS). The ideal quality control material for NGS needs to provide both the tumor mutation gene and the matched background genomic DNA, which is uncataloged in public databases, to accurately distinguish germline polymorphisms and somatic mutations.Methods
We developed a novel KRAS G12V mutant cell line using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9) technique to make up for the deficiencies in existing quality control material and further validated the feasibility of the cell line as quality control material by amplification refractory mutation system (ARMS), Sanger sequencing, digital PCR (dPCR), and NGS.Results
We verified that the edited cell line specifically had the G12V mutation, and the validation results presented a high consistency among the four methods of detection. The three cell lines screened contained the G12V mutation and the mutation allele fractions of G12V‐1, G12V‐2, and G12V‐3 were 52.01%, 82.06%, and 17.29%, respectively.Conclusion
The novel KRAS G12V cell line generated using the CRISPR/Cas9 gene editing system is suitable as a quality control material for all current detection methods and provides a new direction in the development of quality control material.5.
Contrast‐enhanced ultrasound using bolus injections of contrast agent for assessment of postprandial microvascular blood volume in human skeletal muscle 下载免费PDF全文
Kenneth H. Mertz Jacob Bülow Lars Holm 《Clinical physiology and functional imaging》2018,38(5):864-871
Methods capable of measuring blood flow in a tissue‐specific manner are needed. The purpose of this study was to investigate whether contrast‐enhanced ultrasound (CEUS) using bolus injections of SonoVue® is an useful method for assessing postprandial changes in microvascular perfusion in the vastus lateralis muscle. Ten healthy, young subjects were recruited for this study. Six subjects participated in washout and reproducibility protocols to assess washout time of SonoVue® and the reproducibility of the method when measuring microvascular blood volume (MBV). Six subjects (two of which also participated in the washout and reproducibility protocols) participated in exercise and nutrition protocols, to assess the ability of the method to detect changes in MBV in response to these interventions. Intraday variation (coefficients of variation) for MBV indices, as assessed by peak signal intensity (PI) or mean plateau signal intensity (mPI), was high (PI: 19 ± 4·2%; mPI: 23 ± 3·3%). The exercise protocol induced significant increases in MBV indices (PI:+113%, P?0·0001; mPI:+218%, P?0·0001) acutely after exercise cessation. There were no changes in MBV indices in response to feeding during the nutrition protocol (PI: P = 0·51; mPI: P = 0·51). We conclude that CEUS using bolus injections of SonoVue® is not capable of detecting changes in MBV of vastus lateralis in response to feeding. This is probably due to the low reproducibility of the method. However, the method is capable of measuring changes in MBV in response to exercise. This method could therefore be used when investigating exercise‐induced changes in microvascular perfusion. 相似文献
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Effectiveness of a partnership‐based self‐management programme for patients with mild and moderate chronic obstructive pulmonary disease: a pragmatic randomized controlled trial 下载免费PDF全文
Helga Jonsdottir PhD RN Olof R. Amundadottir MS Gunnar Gudmundsson MD PhD Bryndis S. Halldorsdottir MS RN Birgir Hrafnkelsson PhD Thorbjorg Soley Ingadottir MS RN Rosa Jonsdottir MS RN Jon Steinar Jonsson MD Ellen D. Sigurjonsdottir MS Ingibjorg K. Stefansdottir MS RN 《Journal of advanced nursing》2015,71(11):2634-2649