腹壁浅动脉皮瓣的应用解剖及其在头颈部修复中的意义

目的　探讨腹壁浅动脉皮瓣的解剖学基础及其在头颈修复中的意义。 方法　在10例动脉内灌注红色乳胶的成人标本上,解剖观察并测量腹壁浅动、静脉的起源、走行、分支、分布、外径、蒂长及与周围血管的毗邻关系。 结果　腹壁浅动脉的出现率为90%（18侧）,管径为(1.48±0.44) mm（0.85~2.43 　mm）, 蒂长为(4.8±1.18) cm (2.56~7.02 mm),腹壁浅静脉恒定,管径为：(2.33±0.71) mm (1.46~4.05 mm),蒂长为：(5.45±1.2) cm（4.03~8.42 cm）,当腹壁浅动脉缺如或管径细小、位置靠内侧时,外侧旋髂浅动脉上升支代偿性增粗。 结论　腹壁浅动脉皮瓣血供可靠,面积充足,供区损伤小,可选择性的应用于头颈部的多种软组织缺损修复。     

慢性肺疾病早产鼠BALF及肺组织中脂质过氧化的同步研究

目的为探讨高氧致早产儿慢性肺疾病(CLD)发生中支气管肺泡灌洗液(BALF)及肺组织中脂质过氧化的变化规律及相互间关系.方法采用高浓度氧致早产鼠CLD模型为研究对象,应用分光光度计比色法同步测定BALF和肺组织超氧化物岐化酶(SOD)活性及脂质过氧化产物丙二醛(MDA)的含量.结果两组的BALF及肺组织中SOD活性均无差异(P>0.05);而实验组,BALF及肺组织中MDA的水平呈同相变化,即吸高氧3d明显升高(P<0.05),7d达高峰(P<0.01),持续1w后逐渐下降,21d仍高于正常水平(P<0.05).结论肺组织的氧自由基损伤贯穿高氧致CLD发生、发展全过程中;BALF中MDA水平可间接反应肺组织的氧自由基损伤程度.     

旋髂深血管蒂髂骨瓣转位腰骶椎植骨的应用解剖

目的 :为带旋髂深血管蒂髂骨瓣转位腰骶段椎体植骨融合术提供应用解剖学基础。方法 :在3 0具共 60侧灌注红色乳胶的成年尸体上 ,解剖观察旋髂深动脉的起始、走行、分支、分布范围及其毗邻结构 ,测量有关数据 ;摹拟转位情况、测量旋髂深动脉的起点至S1椎体中部、L5～S1椎间、L5椎体中部的距离 ,并用量角器测量旋髂深动脉主干向内转位的角度。结果 :旋髂深动脉起于髂外动脉者占 63 .3 % ,起于股动脉者占 3 6.7%。起点外径 ( 2 .6± 0 .4)mm ,腹壁肌支外径 ( 1.4± 0 .4)mm ,旋髂深动脉的主干延续为髂嵴支 ,外径 ( 1.8± 0 .4)mm ,沿途发出许多小的分支进入髂嵴 ,以最后一个分支作为终点测量其蒂长为( 10 .7± 0 .7)cm ,旋髂深动脉起点至L5椎体中部距离为 ( 11.2± 0 .7)cm ,至L5～S1椎间距离为 ( 10 .1±0 .5 )cm ,至S1椎体中部距离为 ( 9.7± 0 .6)cm ,向内旋转角度为 ( 63 .5± 3 .5 )°。结论 :带旋髂深动脉蒂髂骨瓣转位腰骶段椎体植骨融合具有可行性。     

吸入性损伤患者肺部损伤程度与血清胆碱酯酶的关系分析

目的：回顾性分析吸入性损伤患者肺部损伤程度与血清胆碱酯酶（cholinesterase, CHE）值的关系。方法选取2010年1月至2014年1月在河南大学附属郑州市第一人民医院烧伤科就诊烧伤患者111例。患者分为单纯吸入性损伤组（37例）、吸入性损伤＋肺部感染组（37例）、对照组（37例）,比较单纯吸入性损伤组与对照组CHE情况、单纯吸入性损伤组与吸入性损伤＋肺部感染组间CHE差异,并对吸入性损伤患者的CHE值与临床肺部感染评分（CPIS）情况进行相关性分析。结果吸入性损伤＋肺部感染组、单纯吸入性损伤组CHE值（2065．4±1260．3）U／L 、（5776．3±1273．9）U／L与对照组（8117．8±1064．9）U／L比较差异有统计学意义（t＝8．950、22．320,P＜0．05）;单纯吸入性损伤组CHE值与吸入性损伤＋肺部感染组比较差异有统计学意义（t＝5．803,P＜0．05）;单纯吸入性损伤组与吸入性损伤＋肺部感染组 CHE 值和 CPIS 相关性分析系数分别为0．489、－0．713。结论吸入性损伤患者肺部损伤程度越重,其血清CHE值下降程度就越明显,二者之间存在负相关,烧伤患者血清CHE值在一定程度上可以反映其肺部病情危重程度。     

含十字形结构域蛋白-3对肺癌细胞增殖与迁徙的影响研究

为了解含十字形结构域蛋白-3(JMJD3)对肺癌细胞A549增殖与迁徙的影响,通过JMJD3表达质粒转染A549培养48h后,EDU染色及流式细胞术检测细胞增殖与凋亡,划痕实验检测细胞迁徙,RT-PCR检测p21mR-NA表达。结果显示,JMJD3转染后EDU阳性比例明显高于对照(JMJD3:40.75%±2.07%,对照:20.97%±1.5%,P<0.001);G1期细胞比例降低(JMJD3:47.80%±1.85%,对照:54.60%±0.95%,P=0.005)。转染JM-JD3后p21mRNA表达量为35.89%±3.71%,而对照为91.78%±3.74%,两组p21表达变化明显(P<0.001)。转染JMJD3质粒24h和48h后细胞迁徙距离分别为(0.47±0.27)cm和(0.96±0.40)cm,对照为(0.57±0.22)cm和(1.08±0.33)cm,两组无显著性差异(P>0.05)。JMJD3可促进A549细胞增殖,降低G1期细胞比例并下调p21mRNA表达,但对A549迁徙无明显影响。     

Origin of the T790M mutation and its impact on the clinical outcomes of patients with lung adenocarcinoma receiving EGFR-TKIs

Objective

Recently, a low frequency of de novo T790M mutations existing in tumor tissues before TKIs therapy has been reported. However, the origin of T790M and its impact on clinical outcomes is still being debated. This study aimed to use highly sensitive methods to detect T790M before and after TKIs therapy and investigated the correlation of T790M with clinical prognosis.

Curcumin 对注射脂多糖大鼠肺脏血红素氧合酶-1表达的影响

目的：探讨活化蛋白-1（AP-1）阻断剂curcumin对注射脂多糖大鼠肺组织血红素氧合酶（HO-1）表达的转录调节机制。 方法： 18只大鼠随机分为3组：对照组（经舌静脉注入等量生理盐水）、脂多糖（LPS）组（经舌静脉注入LPS 5 mg·0.5 mL-1·kg-1）和LPS+curcumin组（经腹腔注入AP-1阻断剂curcumin 20 mg·0.5mL-1·kg-1，20 min后再注入LPS 5 mg·0.5 mL-1·kg-1）。给药7 h后杀死动物留取肺组织，应用逆转录聚合酶链反应（RT-PCR）和Westren 印迹法分别检测HO-1 mRNA和蛋白的表达，生化方法检测肺组织匀浆碳氧血红蛋白（HbCO）含量，间接代表肺组织中一氧化碳（CO）含量。 结果： LPS组大鼠肺组织HO-1 mRNA和蛋白的表达水平及肺组织CO含量高于对照组（均P<0.01），而LPS+curcumin组肺组织HO-1 mRNA、蛋白表达及CO含量明显低于LPS组（均P<0.01）。 结论： LPS攻击的大鼠肺组织中HO-1基因转录可能是通过激活AP-1进行调控的。     

Association of small foci of diffuse idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH) with adenocarcinoma of the lung

DIPNECH is regarded as a precursor lesion of neuroendocrine lung tumors, specifically carcinoids. A relationship with lung adenocarcinomas has not been clearly established so far. We present a series of four cases with a concomitant presence of adenocarcinoma and DIPNECH in the lung.     

不同频率脉冲电磁场对人卵巢癌SKOV3细胞增殖、凋亡及迁移的影响

研究不同频率的脉冲电磁场(PEMF)对人卵巢癌SKOV3细胞增殖、凋亡和迁移的影响,为PEMF治疗或预防卵巢癌切除术后发生骨质疏松患者的安全性提供实验参考数据。分别对体外培养的人SKOV3细胞施加不同频率(8、16、32和64Hz)、固定强度为1mT的PEMF,2次/d,30min/次,间隔12h,共3d,以未施加PEMF的细胞作为对照组。分别采用EdU荧光法、Annexin V-FITC荧光法和划痕实验检测SKOV3细胞增殖、凋亡及迁移的情况。结果:频率8Hz的PEMF能明显抑制SKOV3细胞的增殖,并诱导其凋亡。频率8Hz和32Hz的PEMF能明显促进人卵巢癌细胞SKOV3的迁移,而频率16Hz的PEMF却明显地抑制SKOV3细胞的迁移。提示不同频率的PEMF对人卵巢癌细胞SKOV3增殖、凋亡及迁移的影响不同。在临床中对于卵巢癌切除术后的骨质疏松患者,应当慎重考虑PEMF治疗的安全性,并严格选择治疗参数。     

Compatibility of the HINTEGRA prostheses with Korean ankles as evaluated on the basis of cadaveric measurements

The purpose of this study was to obtain anatomical measurements of the distal tibia and talus of Korean ankles and to evaluate, based on those measurements, the compatibility of the HINTEGRA prostheses in the context of total ankle replacement (TAR). We measured the length, width, height, and angles of the distal tibia and talus of 51 cadavers and compared these measurements with the corresponding dimensions of the HINTEGRA prostheses. The male ankles were larger than the female ones as was expected, but their overall shapes did not differ, which fact validates use of the prostheses irrespective of patients' sex. The dimensions of the talus itself did not differ significantly from those previously reported for American whites and blacks and South African whites. This might suggest a possibility that the HINTEGRA prostheses, being used in these countries, would be compatible to Korean ankles, too. In fact, the length range of the talar components was generally compatible with those derived from cadaveric measurements of the trochlea. However, the widths of the tibial and talar components were not completely compatible to Korean ankles. Above all, the length of the large‐sized tibial components was much longer than the largest ankles, which would confine the choice of prosthesis mainly to small‐sized ones for arthroplasty in Korea. Even though these prostheses are currently used, some modifications are needed to extend their usability in Korea, such as shortening and width/length ratio adjustment of the tibial component, and of the talar component accordingly. Clin. Anat. 25:1087–1092, 2012. © 2012 Wiley Periodicals, Inc.     

Polarization fluorescence, spin label and ultracentrifugal studies of specific interaction of low molecular weight proteins with the Fc fragment of human immunoglobulin G

Low mol. wt (about 2000) proteins which were found in normal human serum formed complexes with the Fc fragment of IgG. The interaction constant was not less than 10⁶ 1/mole. These complexes dissociated on dilution of the protein solution to below 2 μM or decreasing the pH below 6. The binding site of these proteins was located in the C_H2 domains in close proximity to the carbohydrate moieties. The dissociation of IgG complexes with these proteins was accompanied by a conformational change in the Fc fragment.     

神经干细胞与透明质酸水凝胶材料生物相容性的研究

目的:评价神经干细胞与改性透明质酸水凝胶支架新材料的生物相容性,研究该透明质酸水凝胶支架作为中枢神经组织工程载体材料的可行性,为用组织工程及干细胞技术治疗中枢神经系统损伤提供基础。方法:以冷冻干燥法制备透明质酸水凝胶材料支架,通过化学接枝法将抗Nogo受体抗体(Anti-NogoR)和多聚赖氨酸(poly-l-lysine,PLL)分子接枝到水凝胶上对其改性,制成新的支架材料。体外培养胚胎13.5d大鼠前脑泡神经干细胞.将神经干细胞与生物支架共培养,通过扫描电镜观察透明质酸水凝胶的内部结构及神经干细胞在支架材料上的粘附与生长情况,通过细胞免疫组织化学技术观察神经干细胞在透明质酸水凝胶材料上的存活与分化情况。结果:制备的透明质酸水凝胶材料具有疏松的三维多孔结构,神经干细胞在支架材料上能够粘附并且有突起长出,生长良好。神经干细胞在支架材料上能够分化。结论:神经干细胞与经过改性的透明质酸水凝胶新材料有很好的生物相容性,能够在材料上存活分化。该新透明质酸水凝胶材料有望作为修复中枢神经损伤的组织工程载体。     

Regions recognized on the light chain of botulinum neurotoxin type A by T lymphocytes of SJL and BALB/c mice primed with inactivated toxin

Lymph node cells (LNC) from SJL (H-2^s) and BALB/c (H-2^d) mice primed once with inactivated botulinum neurotoxin type A (BoNT/A) were examined for their T-cell responses to each of 32 synthetic overlapping peptides (19 residues each, L1–L32) that encompass the entire L chain (residues 1–448) of BoNT/A. LNC of SJL gave strong responses to 6 regions on, L2 (residues 15–23), L10/11/12 (127–173), L19 (253–271) and L21 (281–299), and moderate to weak responses to L9 (113–131), L14/15 (183–215) and L27 (365–383). In BALB/c, LNC gave a substantial T-cell response only against peptide L12 (residues 155–173), and responded very weakly to 9 other peptides. The results were compared with the recognition profiles determined previously in these two strains after multiple BoNT/A injections. Overall responses to the L-chain peptides of T cells in later profiles were found to be somewhat weakened in SJL and stayed essentially at a similar level in BALB/c, although responses to BoNT/A increased. In SJL, response to L10 (127–145) remained the highest in the later profile. Strong responses against L12 (155–173) observed in both strains at early stage were reduced to an insignificant level. Cross-reactivity to tetanus neurotoxin by BoNT/A-specific T cells was observed in SJL but not in BALB/c. Design of an effective synthetic peptide vaccine will require incorporation of both T cell- and Ab-recognition elements of the BoNT molecule. Significance and possible implications of these results on BoNT/A-specific T-cell responses of BoNT-treated patients are discussed.     

Different neuronal localization of aspartate-like and glutamate-like immunoreactivities in the hippocampus of rat, guinea-pig and Senegalese baboon (Papio papio), with a note on the distribution of gamma-aminobutyrate

Antisera were raised in rabbits against aspartate or glutamate conjugated to bovine serum albumin by glutaraldehyde. After immunosorbent purification the antisera reacted selectively with brain protein-glutaraldehyde conjugates of the respective amino acids. These results from model systems encouraged us to employ the antisera to study the distribution of free aspartate and glutamate in brain tissue. The aspartate antiserum produced intense staining of interneurons and deep hilar neurons and modest labelling of pyramidal and granular cells in the hippocampal formation of rats, guinea-pigs and baboons perfusion-fixed with glutaraldehyde. In contrast, glutamate-like immunoreactivity was generally high in pyramidal and granular cells and low in interneurons. In hippocampal slices immersion fixed in glutaraldehyde after being soaked in Krebs' solution aspartate-like and glutamate-like immunoreactivities were lost from perikarya and dendrites. The staining that remained occurred in nerve terminal-like dots and matched the distribution of the major excitatory fiber systems, except that only glutamate-like immunoreactivity, and not aspartate-like immunoreactivity, was concentrated at the site of the mossy fiber terminals, and that aspartate-like but not glutamate-like immunoreactivity occurred between the granular and pyramidal cell bodies. The present technique specifically demonstrates aspartate and glutamate in glutaraldehyde-fixed tissue. We suggest that in perfusion-fixed material the staining intensities reflect the total concentrations of the amino acids (i.e. the "metabolic pool" plus the "transmitter pool"). In immersion-fixed hippocampal slices the "transmitter pool" may be preferentially visualized.     

Enzyme-linked immunosorbent assay (ELISA) with covalently bound protein on glass tubes: 1. Stable antigenicity and binding of IgG as a model antigen after repeated use

A modification of the ELISA procedure is described. The system is based on the covalent binding of protein to glass tubes. Human IgG was used as model antigen. Optimal conditions were tested for the removal of alkaline phosphatase-labeled antibodies from their antigen. Under such optimal conditions, a regenerable system could be created which exhibited many advantages, as compared with conventional ELISAs with antigens absorbed unspecifically to plastic surfaces. The advantages are: 1. A higher density of IgG as model antigen on the solid phase, i.e., 1 molecule IgG per 94 nm2 with glass tubes as compared with 110 nm2 with polystyrene (PS) or 143 nm2 with polyvinylchloride (PVC) microtiter plates. 2. A much smaller unspecific absorption of less than 1% as compared with 39% with PS-plates or 16% with PVC-plates treated under identical conditions. 3. A higher stability of the binding of the model antigen to the solid phase, i.e., a drastically reduced protein loss of less than 6% during the first ELISA procedure (including the regeneration) and of less than 1% during the subsequent ELISA and regeneration cycles with glass tubes as compared with 46% (PS plates) or 55% (PVC plates). 4. A smaller intraday variation coefficient of the ELISAs of 4.8% with glass tubes as opposed to 9.7% or 7.5% with PS or PVC plates respectively. The system with covalently bound antigens on glass tubes could be used in at least 20 consecutive measuring cycles. In five consecutive cycles, a protein loss of less than 5% was observed and the interday variation coefficient of the ELISA reaction was smaller than 5% using the same tubes repeatedly. Our results indicate that covalent linkage of protein can improve ELISA and lead to repeatedly usable systems as long as the antigen is stable against the regeneration procedure. Such an ELISA system may be helpful with highly purified proteins.