IL-8和皮质醇与特发性早产关系的研究

目的　探讨白细胞介素 8和皮质醇含量的变化及其与特发性早产的关系。方法　随机选取 32例特发性早产孕妇作为病例组 ,及 15例同期正常孕妇作为对照组 ,采用ELISA法检测两组孕妇血清、羊水、尿液中的IL - 8含量 ,采用放射免疫法测定血清、羊水中的皮质醇含量。结果　早产组孕妇血清、羊水和尿液中IL - 8含量分别为 (0 .2 8± 0 .13) μg/ml,(0 .2 9± 0 .12 ) μg/ml和 0 .18± 0 .15× 10 -2g/mol肌酐 ,明显高于对照组的 (0 .16± 0 .0 7) μg/ml,(0 .17± 0 .0 9) μg/ml,(0 .0 5± 0 .0 2 ) g/mol肌酐 ;早产组孕妇血清和羊水中皮质醇含量分别为 (75 6 .38± 4 0 2 .35 ) μg/ml和 (96 .12± 89.4 9) μg/ml,明显高于对照组的 (5 12 .6 5± 14 3.2 3) μg/ml和 (37.10± 17.79) μg/ml。早产组孕妇血清、羊水中的IL - 8与皮质醇含量均呈正相关 (r1=0 .4 2 71,P <0 .0 5 ;r2 =0 .5 388,P <0 .0 5 )。结论　IL - 8和皮质醇含量在早产的发病中发挥着重要的作用     

L-精氨酸对接振家兔外周血管功能保护作用的研究

目的探讨L 精氨酸对后肢接振家兔外周血管功能的保护作用及意义。方法将家兔随机分为单纯接振组 (A组 ) ,L 精氨酸组 (B组 )和对照组 (C组 ) ,并进行接振试验。从试验第 11天起 ,每隔 2d给予B组L 精氨酸 2 0mg/kg ,分别测定各组血浆一氧化氮 (NO)、内皮素 (ET)浓度进行测定分析。结果试验前及试验后第 10天、2 0天、3 0天 ,A组NO浓度分别为 ( 172 .40 0± 12 .3 5 0 ) μmol/L、( 167.915± 7.879) μmol/L、( 15 0 .0 88± 7.45 3 ) μmol/L、( 13 2 .3 13± 8.747) μmol/L ,ET浓度分别为 ( 4 3 .5 75±8.166) μg/L、( 4 6.2 13± 5 .44 8) μg/L、( 5 3 .788± 6.0 3 7) μg/L、( 66.613± 9.485 ) μg/L ;B组浓度分别为 ( 172 .85 0± 13 .3 2 3 ) μmol/L、( 167.788± 10 .5 3 1) μmol/L、( 164 .12 5± 8.3 80 ) μmol/L、( 161.813± 7.63 9) μmol/L ,ET浓度分别为 ( 4 3 .5 13± 4.90 1) μg/L、( 4 7.175± 7.481) μg/L、( 4 7.0 2 5± 5 .0 2 6) μg/L、( 4 7.60 0± 4.883 ) μg/L。随接振时间延长 ,A组NO浓度有明显降低、ET浓度有明显升高趋势 (P <0 .0 1) ;B组也有类似变化 ,但变化幅度较小。结论L 精氨酸可通过影响接振家兔血管内皮活性物质而保护外周血管功能。     

杭白菊对小牛血管平滑肌细胞凋亡及其抗氧化性研究

目的 :探讨杭白菊提取液对小牛主动脉血管平滑肌细胞 (VSMC)凋亡的影响及可能的作用机制。方法 :取小牛主动脉进行体外 VSMC培养 ,加入杭白菊提取液干预 ,用膜联蛋白 Annexin检测法 ,在流式细胞仪下观察VSMC的凋亡现象 ,同时取上清液在分光光度仪下测定超氧化物歧化酶 (SOD)、丙二醛 (MDA)。结果 :1在流式细胞仪下观察到加入杭白菊提取液后 VSMC凋亡数量减少 ,随着杭白菊提取液浓度的加大 ,细胞凋亡从 (4.4 2 5±0 .6 2 4 ) %降至 (2 .875± 0 .6 4 0 ) % ;2随着杭白菊提取液浓度的增加 ,SOD值由 (1.6 83± 0 .149)× 10 4 U/ L升高到(2 .2 97± 0 .2 30 )× 10 4 U/ L、MDA值由 (16 6 .4 5 4± 5 6 .80 5 ) μmol/ L下降至 (73.0 6 8± 2 7.2 0 3) μmol/ L。结论 :杭白菊提取液具有抑制小牛 VSMC凋亡及抗氧化作用 ,并呈浓度依赖性。     

140例Graves’病患者血清HA、LN、PCⅢ、IV．C含量的临床分析

目的 :应用血清中的HA、LN、PCⅢ、IV .C含量 ,来判断甲状腺功能亢进对肝脏功能的影响。方法 :采用放射免疫法测定 14 0例Graves’病患者和 33例健康体检者的血清HA、LN、PCⅢ、IV .C含量。Graves’病患者分为初诊未用ATD组 ( 5 9例 )和曾用ATD组 ( 81例 )。结果 :血清HA含量未用ATD组 (均数±标准差 119.4 3± 10 7.0 8μg/L)明显高于对照组 ( 78.30±5 8.4 4 μg/L) ,P <0 .0 5 ,而曾用ATD组 ( 10 8.4 9± 6 6 .94 μg/L)与对照组之间无显著性差异 ;血清LN、PCⅢ、IV .C含量在所有的病人均显著高于对照组 ,P <0 .0 0 1,未用ATD组分别为 ( 2 0 3.18± 98.5 5 )、( 2 0 9.0 6± 10 8.6 2 )、( 117.79± 6 3.39) μg/L ,曾用ATD组分别为 ( 190 .0 3± 79.37)、( 172 .36± 10 4 .4 5 )、( 119.0 2± 4 7.38) μg/L ,对照组分别为 ( 130 .88± 15 .12 )、( 74 .5 9± 2 1.87)、( 6 4 .5 6± 2 7.6 4 ) μg/l。 结论 :Graves’病患者无论是否接受过ATD治疗 ,血清LN、PCⅢ、IV .C增高 ;HA在初诊病人升高 ,而接受过ATD治疗的病人正常 ,也许与ATD的免疫调节有关。     

尿嗜酸粒细胞蛋白-X在儿童哮喘诊断和病情监测中的意义

分别用放射免疫法测定儿童哮喘急性发作组、缓解组和正常对照组 U - EPX浓度。结果急性发作组、缓解组和正常对照组 U- EPX浓度分别为 14 5 .0± 4 0 .6 μg/ mmol肌酐、91.0± 2 3.4 μg/ mmol肌酐、5 2 .0± 15 .4 μg/mmol肌酐 ,急性发作组和缓解组、对照组比较差异极有显著性 (P<0 .0 1) ;缓解组和对照组比较差异有显著性 (P<0 .0 5 )。提示 ,U- EPX在儿童哮喘诊断和病情监测中有重要临床参考价值     

妊娠晚期无畸形单胎妊娠母血甲胎蛋白水平的临床意义

为了探讨妊娠晚期无畸形单胎妊娠母血AFP水平的临床意义 ,用放射免疫法检测了 10 2例高危及正常妊娠晚期孕妇血清甲胎蛋白。前瞻性观察妊娠结局。结果 :高危和正常妊娠组母血AFP均值分别为 :(12 3 .45±33 .2 4) μg/L ,(99 18± 2 5 .6 8) μg/L ,差异显著 (P <0 .0 1)。以高出正常晚妊孕妇母血AFP 2个标准差 15 0 μg/L为临界值 ;≥ 15 0 μg/L的孕妇羊水胎粪污染、足月小样儿、Ⅲ级胎盘发生率均较母血AFP低于此值的孕妇增高(P <0 .0 5 )。且前者新生儿Apgar评分≤ 7分 ,E/C比值≤ 10的发生率较后者明显增高 (P <0 .0 1)。提示 :妊娠晚期无畸形单胎妊娠母血AFP值可作为间接反应胎盘功能 ,预测妊娠结局的参数之一。     

肝细胞生长因子体外对肝癌细胞HepG2增殖和凋亡的影响

目的:探讨肝细胞生长因子(HGF)对肝癌细胞 HepG2 增殖和凋亡的影响. 方法: 人肝癌细胞株 HepG2加入不同浓度HGF(0, 1, 10, 50 μg/L) 培养2, 4, 6 d,应用MTT 法检测细胞增殖情况,流式细胞仪检测细胞周期及凋亡百分率的变化,免疫细胞化学法检测增殖细胞核抗原(PCNA)的表达. 结果: HGF 抑制 HepG2 细胞增殖呈剂量及时间依赖性,并诱导其凋亡[50 μg/L HGF,培养时间为4, 6 d,其抑制率: (30.7±10.2)%, (49.8±11.1)% vs 1 μg/L HGF: (10.7±11.2)%, (4.2±9.4)%; P《0.05, P《0.01]. 随着HGF浓度增大S期细胞明显减少[试验组10, 50 μg/L HGF: (7.5±4.4)%, (7.8±1.6)% vs对照组0 μg/L HGF: (16.6±2.8)%; P《0.05, P《0.01],同时可见G0/G1 期细胞累积现象[试验组10, 50 μg/L HGF: (80.5±3.2)%, (73.1±3.9)% vs对照组0 μg/L HGF:(59.6±6.2)%; P《0.05, P《0.01]. 试验组 G1 峰前出现明显的凋亡峰,凋亡率亦较对照组显著增加(P《0.05). 且试验组细胞 PCNA 表达明显高于对照组. 结论: HGF 对肝癌细胞 HepG2 有抑制增殖和诱导其凋亡的作用,诱导细胞 G0/G1 期阻滞为可能机制之一.     

根除幽门螺杆菌对消化性溃疡患者胃泌素表达水平的影响

目的　通过观察根除幽门螺旋杆菌对消化性溃疡患者胃泌素基因表达水平及血清胃泌素分泌影响 ,来探讨Hp可能的致病机理。方法　对Hp阳性的 6 1例消化性溃疡患者 ,给予质子泵抑制剂 (洛赛克 2 0mg/d)、甲硝唑(0 8g/d ,分 2次服 )和阿莫西林 (1 0 g/d ,分 2次服 )联合抗Hp治疗 2周 ,治疗前及治疗后分别检测胃泌素及胃泌素基因。结果　在GU患者中 ,Hp根除组胃泌素基因表达水平从治疗前的 (0 36± 0 11) μg/gpro变为治疗后的(0 2 7± 0 0 9) μg/ gpro (P <0 0 1)。在DU患者中 ,Hp根除组胃泌素基因表达水平和胃泌素水平分别从治疗前的(0 4 2± 0 16 ) μg/gpro和 (38 2± 11 3) pmol/L变为治疗后的 (0 2 7± 0 17) μg/ gpro和 (30 3± 10 6 )pmol/L ,治疗前后比较差别均有非常显著性意义 (P均 <0 0 1)。结论　Hp感染导致胃泌素基因表达水平及胃泌素分泌增加与十二指肠溃疡的发生关系更为密切 ,根除Hp可使胃泌素基因的表达水平明显下降。胃溃疡患者中 ,胃泌素分泌的调节除与胃泌素基因表达相关外 ,还受胃酸的负反馈等其他机制的影响。     

常年性变应性鼻炎患者血清ECP水平对气道高反应与哮喘的预测

目的　探讨常年性变应性鼻炎患者血清嗜酸细胞阳离子蛋白 (ECP)水平的临床意义。方法　应用PharmaciaCAPSystemECPFEIA荧光酶标法测定正常人 (第 1组 )、常年性变应性鼻炎组胺激发试验阴性患者 (第 2组 )、常年性变应性鼻炎组胺激发试验阳性患者 (第 3组 )和常年性变应性鼻炎伴支气管哮喘患者 (第 4组 )的血清ECP水平。结果　血清ECP值 :第 1组 (7. 84± 3 .2 1) μg/L ,第 2组 (7 .96± 35 ) μg/L ,第 3组为 (2 0 . 5 5± 2. 2 1) μg/L第 4组为 (2 8 .5 5± 6 . 38) μg/L ,第 1组和第 2组相比差异无统计学意义 (P >0 . 0 5 ) ,第 3组、第 4组和第 1组相比 ,差异有显著性 (P <0 .0 1)。结论　血清ECP水平可作为判断是否有气道高反应和哮喘的发病客观依据 ;它可以作为一个动态观察和监测变应性鼻炎是否发展为气道高反应和哮喘的简单而又客观的指标。     

肿瘤病人舌苔变化与唾液中人上皮生长因子的关系研究

目的 :研究肿瘤病人舌苔变化与唾液中人上皮生长因子h -EGF含量的关系。方法 :采用1 2 5 I放射免疫分析法 (RIA )。结果 :15 2例肿瘤病人唾液中h -EGF含量为 (4.0 70± 1.0 ) μg L ,明显高于正常人 (3.0 71±1.7) μg L(P <0 .0 1) ,其中 10 2例厚苔者 (占 6 7.1% )的h -EGF含量为 (4.2 70± 1.7) μg L ,也明显高于 5 0例薄苔者 (占 32 .9% )的h -EGF含量 (3.6 91± 1.2 ) μg L (P <0 .0 1)。 结论 :唾液中h -EGF含量与肿瘤病人舌苔增厚密切相关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.