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C纤维不是电针镇痛的主要传入纤维,而是弥散性伤害性抑制控制的主要纤维 总被引:12,自引:3,他引:12
<正> 电针刺激通过何种纤维传入中枢产生镇痛作用,至今仍有争论。范少光等采用我室常用电针参数,以大鼠行为反应为指标,观察到电针(Electroacupuncture,EA)镇痛信息主要不是由 C 纤维传入。本工作用辣椒素选择性阻断 C 纤维传导后,观察了 EA 和伤害性热刺激(Noxious heat,NH)对脊髓广动力范围(Wide DynamicRange,WDR)神经元抑制作用的影响,进一步对上述问题作探讨。 相似文献
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Hirano T Zeredo JL Kimoto M Moritaka K Nasution FH Toda K 《The American journal of Chinese medicine》2008,36(3):569-577
The present study was conducted to clarify the role of the anterior cingulate cortex (ACCX) in acupuncture analgesia. Experiments were performed on 35 female Wistar albino rats weighing about 300 g. Single unit recordings were made from ACCX neurons with a tungsten microelectrode. Descending ACCX neurons were identified by antidromic activation from electrical shocks applied to the ventral part of the ipsilateral PAG through a concentric needle electrode. Cathodal electroacupuncture stimulation of Ho-Ku (0.1 ms in duration, 45 Hz) for 15 min was done by inserting stainless steel needles bilaterally. An anodal silver-plate electrode (30 mm x 30 mm) was placed on the center of the abdomen. Naloxone (1.0 mg/kg, i.v.) was used to test whether changes of ACCX activities were induced by the endogenous opioid system. Data were collected from a total of 73 ACCX neurons. Forty-seven neurons had descending projection to the PAG, and the other 26 had no projections to the PAG. A majority of descending ACCX neurons were inhibited by electroacupuncture stimulation. By contrast, non-projection ACCX neurons were mainly unaffected by electroacupuncture. Naloxone did not reverse acupuncture effects on the changes of ACCX neuronal activities. Acupuncture stimulation had predominantly inhibitory effects on the activities of descending ACCX neurons. Since the functional connection between ACCX and PAG is inhibitory, electroacupuncture caused disinhibition of PAG neurons, whose activity is closely related to descending antinociception to the spinal cord. This disinhibitory effect elicited by acupuncture stimulation is thought to play a significant role in acupuncture analgesia. 相似文献
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目的:观察电针在取卵术中辅助镇痛的效果,并对杜冷丁不良反应的改善作用进行观察,为电针在取卵术中的应用提供科学、有效的临床依据.方法:将134例接受体外受精-胚胎移植(IVF-ET)助孕的门诊患者随机分为针药组和药物组,每组67例.两组患者均于术前30 min肌肉注射杜冷丁50 mg,针药组注射杜冷丁后给予针刺百会、疼痛穴(右)、三阳络(右)、足三里(右)及耳穴子宫(右耳),得气后在疼痛穴和三阳络行电针刺激,至取卵手术结束.药物组注射杜冷丁30 min后进行取卵手术.观察两组患者术中的疼痛等级和疼痛积分,记录术中及术后1h、2h不良反应发生情况.结果:针药组镇痛优良率为97.0%(65/67),药物组为92.5%(62/67),组间镇痛疗效差异有统计学意义(P<0.05);针药组在疼痛等级和疼痛积分方面明显优于药物组(均P<0.05);针药组术中眩晕、出汗、恶心等不良反应发生例数明显少于药物组(均P<0.05);术后1h针药组眩晕、恶心、腹痛例数少于药物组(均P<0.05),术后2h眩晕、恶心、呕吐、腹痛例数少于药物组(均P<0.05).结论:在B超引导下的经阴道取卵术中,电针在术中辅助镇痛效果好,并在缓解杜冷丁术中及术后不良反应方面有明显的作用. 相似文献
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In order to understand the role of substance P (SP) in the brain and the relationship between SP and enkephalins in the electroacupuncture analgesia (EA), we have observed the influence of SP-antagonist, (D-Arg', D-Phe5, D-Trp7.9, Leu11) -SP (DADPDTL) injected intracerebroventricularly (icv) on EA and the change of the level of SP in the brain regions of the rat during EA. We have made a further observations on the influences of the naloxone (NX) on the che change of the content of SP induced by EA and DADPDTL on the increase in Leu-enkephalins (LEK) induced by EA. The Wistar rats were used in the experiment. The latency of the tail flick, immersing the tip of rat tail (4 cm) into hot-water of 50 degrees C, was taken as the pain threshold. The drugs were injected icv via plastic cannulae implanted in the bilateral ventricles. The EA was applied to the point of "Zusanli" (S36). The contents of SP and LEK were determined radioimmunoassay in the hypothalamus, mid-brain, striatum and pons-medulla-oblongata. The pain threshold was increased by 48 +/- 9% (P less than 0.01) after EA. But icv injection of DADPDTL decreased the pain threshold by 14 +/- 7% after EA. The result suggests that DADPDTL can antagonize the effect of EA and that SP in the brain is involved in EA. After EA the contents of SP in the hypothalamus and mid-brain of the rats were decreased by 29% and 28% in comparison with that of the control group respectively (both of them, P less than 0.05), but the contents of SP in the striatum and pons-medulla-oblongata had no significant change.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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目的:探讨粗纤维在电针"四白"穴对内脏痛的镇痛效应中的作用及其传入途径。方法:SD大鼠36只,随机分为空白组,内脏痛组、电针组、眶下神经切断组,神经蛇毒组及生理盐水组,每组6只。切断大鼠双侧眶下神经以及双侧眶下神经鞘内注射神经蛇毒选择性破坏A类纤维,然后电针双侧"四白"穴,再腹腔注射乙酸造成内脏痛模型,观察大鼠内脏痛的行为学变化(扭体反应)以及孤束核(NTS)和三叉旁核(PTN)的c-fos表达。结果:电针组大鼠扭体次数较内脏痛组明显减少(P0.01),眶下神经切断组大鼠扭体次数较电针组明显增加(P0.01),神经蛇毒组大鼠扭体次数少于内脏痛组(P0.05)。电针组NTS的c-fos表达较内脏痛组明显减少(P0.05),眶下神经切断组较电针组明显增加(P0.01),神经蛇毒组显著低于内脏痛组(P0.05)。电针组PTN的c-fos表达较内脏痛组明显增加(P0.01),眶下神经切断组较电针组明显减弱(P0.01),神经蛇毒组较眶下神经切断组和内脏痛组明显增强(P0.01)。结论:粗纤维不是电针"四白"穴对内脏痛大鼠镇痛效应的主要传入纤维,口面部的躯体感觉传入可能经PTN中继后与内脏的感觉传入在NTS发生汇聚并进行整合,从而产生镇痛作用。 相似文献
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电针对应激性胃黏膜损伤大鼠胃黏膜肠三叶因子基因表达的影响 总被引:6,自引:0,他引:6
目的:探讨电针预处理对胃黏膜损伤大鼠胃黏膜组织肠三叶因子(intestinal trefoil factor,ITF)基因表达的影响及意义。方法:将40只大鼠随机分为空白组、模型组、胃经组、胆经组。空白组不做任何处理,而模型组仅捆缚7d后造模,胃经组、胆经组须先捆缚电针7d后再造模。造模采用水浸束缚法,水浸束缚10h,经相应处理后检测胃黏膜损伤指数(UI),同时取各组大鼠胃黏膜组织,采用RT-PCR方法测胃黏膜组织肠三叶因子基因(ITF mRNA)表达。结果:①胃经组、胆经组与模型组的UI比较差异有显著或非常显著性意义(P〈0.05或P〈0.01),说明造模成功;②电针可提高胃黏膜组织ITF mRNA表达水平,胆经组较模型组ITF mRNA表达增高,胃经组较胆经组ITF mRNA表达非常显著升高(P〈0.01)。结论:电针对胃黏膜组织特异性调整作用,与肠三叶因子基因表达差异有关。 相似文献
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Lee SE Seo JM Liu J Hong MS Lee YW Lee JY Song KH Kim DH 《The American journal of Chinese medicine》2006,34(1):69-76
This study was performed to clarify the differences of the body heats between electroacupuncture analgesia (EA) and anesthesia by ketamine hydrochloride (ketamine anesthesia) in dogs. Nine clinically healthy dogs were divided into ketamine anesthesia (control: 5 heads) and EA (experimental: 4 heads) groups, respectively. The acupoints GV-5 and Bai-Hui were used. The infrared thermographic system was used to determine the body heats. The body heats was determined at areas such as the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr) and ventrocaudal (VCd) regions, on pretreatment, 10, 20, 30, 50 and 90 minutes after treatments, respectively in control and experimental groups. The body heats showed decreasing tendency until 30 minutes after ketamine injection, and then showed increasing pattern until 90 minutes after at all areas investigated in the control group. However, no significant differences of the body heats in the DCr, DCd, VCr and VCd regions were found in the control group. On the other hand, the body heats showed increasing tendency until 30 minutes, and then showed decreasing pattern until 90 minutes after EA, in the experimental group. The significant difference was observed at 30 minutes in the DCr region, and also at 10, 20 and 30 minutes in the DCd regions in the experimental group (p < 0.05). The significant differences of the body heats were detected at 20 minutes in the DCr region, at 30 minutes in the DCd region and at 30 minutes in the VCd region between the experimental and control groups (p < 0.05). In conclusion, EA increases of the body heat in the contrary to that of ketamine anesthesia. 相似文献
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紫草素通过线粒体途径诱导A375-S2细胞凋亡 总被引:5,自引:0,他引:5
目的 :研究紫草素诱导人黑色素瘤A375-S2细胞凋亡的机制。方法 :MTT法测定紫草素对A375-S2细胞的生长抑制实验 ,形态学观察分析细胞凋亡的形态学变化 ,DNA电泳研究细胞死亡的机制 ,免疫印迹实验检测相关蛋白的表达。结果 :紫草素可明显地抑制A375-S2细胞的生长 ,在 25~40 μmol·L-1之间呈明显的量效关系和时效关系。 10μmol·L-1紫草素可诱导A375-S2细胞凋亡 ,琼脂糖凝胶电泳可见典型的DNA“梯状”条道。caspase-3和caspase-9的抑制剂阻止紫草素诱导的凋亡 ,紫草素可诱导A375-S2细胞caspase-9的活力增加 ,免疫印迹结果显示的上调的Bax和下调的Bcl-XL 表达证实了紫草素诱导的A375-S2细胞的凋亡是通过线粒体途径发挥作用的。结论 :10μmol·L-1紫草素可明显地诱导A375-S2细胞凋亡 ,并经历了caspase-9激活的线粒体信号转导途径。 相似文献
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Shieh DE Cheng HY Yen MH Chiang LC Lin CC 《The American journal of Chinese medicine》2006,34(2):245-261
Acute lymphoblastic leukemia (ALL), especially T-acute lymphoblastic leukemia (T-ALL), is a common childhood malignant neoplastic disorder. Chemotherapy agents, particularly those that can induce apoptosis, are the major intervening strategy in the treatment of ALL. In this study, we investigated in T-ALL cell line, CCRF-CEM, the in vitro cytotoxic effect and the mechanism of action of baicalin, a compound extracted from Scutellaria baicalensis Georgi and S. rivularis Benth (Labiateae). Results demonstrated that baicalin displayed a remarkable cytotoxic effect in CCRF-CEM, with an IC(50) value of 10.6 microg/ml. It triggered apoptotic effect by fragmentizing cellular DNA and arrested the cell cycle at G(0)/G(1) phase. Baicalin (37.5 microg/ml) had not effected the expression of p53 and Fas protein. It was shown to decline the expression of Bcl-2 (22.0 pg/ml), which consequently caused the loss (52.7%) of transmembrane potential (Delta Psi m) in the mitochondria after 72 hours of treatment. Baicalin (37.5 microg/ml) also elevated the amount of cytosolic cytochrome c (19.2 microg/ml), which finally triggered the activation of caspase-3 (50.1 pmol/min). In conclusion, baicalin was found to induce apoptosis in T-ALL cell lines through multiple pathways. This finding encourages further investigation of baicalin in its role as a potential candidate for chemotherapeutic agents in T-ALL. 相似文献
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Spinal serotonergic system is partially involved in antinociception induced by Trigonella foenum-graecum (TFG) leaf extract 总被引:1,自引:0,他引:1
It has been reported that Trigonella foenum-graecum (TFG) extract exerts analgesic, anti-inflammatory and anti-pyretic effects in different experimental models. The major objective of this paper was to investigate the site and mechanism of the analgesia induced by Trigonella foenum-graecum extract. We studied the analgesic effects of different doses of Trigonella foenum-graecum extract after i.p., i.t. and i.c.v. administration in formalin test, using male NMRI rats (200-250 g). Trigonella foenum-graecum extract showed analgesic effects in i.p. (1 g/kg) and i.t. (0.5, 1, and 2 mg/rat) (P < 0.05 in all groups) but not in i.c.v. (1 and 3 mg/rat) administrations. Based on the similarities between the effects of Trigonella foenum-graecum extract with those of nonsteroidal anti-inflammatory drugs (NSAIDs) and the role of 5-HT system in analgesic effects of NSAIDs, we tried to investigate the role of spinal 5-HT system in analgesic effects of Trigonella foenum-graecum extract. After lesioning of spinal 5-HT system by 5,7-dihydroxytryptamine (5,7-DHT), it was shown that the analgesic effect of Trigonella foenum-graecum extract (0.5 and 3 mg/rat) in the second phase of formalin test, was abolished completely and reduced relatively after using a low-dose (0.5 mg/rat) and a high-dose (3 mg/rat), respectively (P < 0.05). So, the antinociception partially remained (P < 0.05) after using the latter dose. Meanwhile, administration of naloxone (2mg/kg, i.p.) had no effect on the Trigonella foenum-graecum extract (1 g/kg, i.p.) analgesia. In conclusion, this study confirms the central action of Trigonella foenum-graecum extract and that spinal 5-HT system is partially involved in the analgesia induced by it in the second phase of formalin test and also indicates for co-existence of other analgesic mechanism(s). 相似文献
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督脉电针治疗大鼠全横断性脊髓损伤的实验研究 总被引:25,自引:2,他引:25
目的:探讨督脉电针对大鼠全横断性脊髓损伤的行为和结构修复的影响。方法:用BBB评分法和爬网格试验检测全横断性脊髓损伤及督脉电针治疗后大鼠的运动功能,荧光金逆行标记法检测再生神经元,用生长相关蛋白-43(GAP-43)免疫组织化学法观察脊髓损伤区周围神经元GAP-43的表达。结果:督脉电针治疗后大鼠后肢运动功能有明显地恢复,损伤区脊髓组织退变减轻,躯体感觉运动区内锥体细胞层及红核内的神经元密度增大,脊髓组织内GAP-43阳性神经元增多,在脊髓横断头侧端灰质内、躯体运动感觉区及红核内有少量被标记细胞。结论:督脉电针治疗可减轻脊髓损伤后的继发性损伤,促进脊髓下行纤维再生和脊髓功能恢复。 相似文献
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Objective
To observe the effect of electroacupuncture (EA) on the expression of erythropoie-tin-producing hepatocyte receptor B2 (EphB2) in the cortex around the infracted area of middle cerebral artery occlusion (MCAO) rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia.Methods
A total of 180 male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups: postoperative 3 d, postoperative 14 d and postoperative 21 d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride (TTC) staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators.Results
The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group (P<0.05). The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells (EphB2+) around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group (P<0.05), and the positive cell number was higher than that in the sham operation group (P<0.01). Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2+ and postsynaptic density-95 positive (PSD-95+) were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group (P<0.05).Conclusion
Electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.17.
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《现代中西医结合杂志》2016,(19)
目的研究黄芪多糖对H_2O_2所致乳鼠心肌细胞氧化应激损伤的影响,并探讨其可能作用机制。方法分离并培养乳鼠心肌细胞72 h后分为空白对照组、H_2O_2组、黄芪多糖20μmol/L+H_2O_2组、黄芪多糖40μmol/L+H_2O_2组、黄芪多糖80μmol/L+H_2O_2组,每组设10个复孔。每组给予相应干预24 h后,采用MTT法检测细胞存活率,采用紫外-可见分光光度计检测细胞中抗氧化酶[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)]活性,采用氧敏感荧光探针DCFH-DA检测氧自由基(ROS)含量,采用全自动生化分析仪检测培养液中心肌酶[谷草转氨酶(AST)、磷酸肌酸激酶(CPK)、乳酸脱氢酶(LDH)]和丙二醛(MDA)含量,通过流式细胞仪检测细胞凋亡状况并计算凋亡率,采用免疫印迹法检测细胞核因子-κB(NF-κB)蛋白表达情况并进行半定量分析。结果与H_2O_2组比较,黄芪多糖40μmol/L+H_2O_2组和黄芪多糖80μmol/L+H_2O_2组细胞存活率及细胞中SOD、GSH-Px、CAT活性显著升高(P均0.05),ROS含量显著降低(P0.05),培养液中AST、CPK、LDH和MDA含量及细胞凋亡率、NF-k B蛋白表达量均显著降低(P均0.05);黄芪多糖80μmol/L+H_2O_2组细胞存活率及细胞中SOD、GSH-Px活性均显著高于黄芪多糖40μmol/L+H_2O_2组(P均0.05),CPK、MDA含量及细胞凋亡率、细胞中NF-κB蛋白表达量均显著低于黄芪多糖40μmol/L+H_2O_2组(P均0.05)。结论黄芪多糖可能通过提高心肌细胞存活率、改善抗氧化酶活性、提高ROS清除能力、降低心肌酶含量、抑制细胞凋亡、下调NF-k B蛋白表达而对H_2O_2诱导乳鼠心肌细胞氧化应激损伤起保护作用,且高剂量效果更好。 相似文献
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Téllez MG Rodríguez HB Olivares GQ Sortibrán AN Cetto AA Rodríguez-Arnaiz R 《Journal of ethnopharmacology》2007,111(1):182-189
Equisetum myriochaetum is a Mexican plant used in folk medicine to treat kidney diseases and type 2 diabetes mellitus. The main constituents of the phytoextract are flavonol glycosides (kaempferol), phytoesterols and carbohydrates. In this study, phytotherapeutic extracts from Equisetum myriochaetum were investigated for genotoxicity in the in vivo wing spot test in Drosophila melanogaster and in the in vitro human micronucleus test. No acute toxicity of the phytoextract could be determined in Drosophila or in human lymphocytes in culture, ranging from 0.78 microg/ml to 3700 microg/ml for the wing assay and between 12.5 microg/ml and 500 microg/ml for the micronucleus test. The Drosophila wing somatic mutation and recombination test (SMART) was applied in the standard version with basal biotransformation activity as well as in a variant version with increased cytochrome P450-dependent bioactivation capacity. The ranges of exposure concentrations for these genotoxicity experiments were between 0.78 microg/ml and 500 microg/ml. The human micronucleus test in vitro was performed with cultured lymphocytes obtained from four healthy donors. The concentrations assayed for these experiments ranged from 12.5 microg/ml to 500 microg/ml. No statistically significant increase was observed between treated series when compared with a concurrent negative (water solvent) control series in either assay. The results demonstrate clearly that the phytotherapeutic extract from Equisetum myriochaetum, under the experimental conditions tested, is not genotoxic in the in vivo experiments or in the in vitro studies. 相似文献
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Rats were randomly divided into 4 groups: Control, Electro-acupuncture (EA), Naloxone and Naloxone plus EA. The content of noradrenaline (NA) and dopamine (DA) in brain stem, diencephalon and telencephalon were determined by spectrofluorometry. It was found that the pain threshold increased and the NA content in the telencephalon decreased and the DA level in the brain stem increased markedly after EA. Naloxone injection partially decreased the analgesic effect of EA and abolished the EA-induced elevation of DA in the brain stem and attenuated the NA level in the diencephalon after EA. These results suggest that morphine receptor may play an important role in EA analgesia and this may be partially carried out through the brain catecholamine system. 相似文献