Hpa和nm23在大肠癌的表达及其与侵袭转移的相关性

目的 探讨乙酰肝素酶Hpa及转移抑制基因nm23在大肠癌组织中的表达及其与临床病理特征的关系.方法 采用免疫组化EnVision法检测54例原发性大肠癌组织中Hpa及nm23蛋白的表达.结果 Hpa和nm23蛋白在大肠癌中的阳性表达率分别为48.1%、63.0%;nm23和大肠癌肿瘤大小无明显相关(P>0.05),而Hpa与肿瘤大小显著相关(P<0.01),nm23和Hpa蛋白与肿瘤浸润深度、淋巴结转移和肝转移有关(P<0.05,P<0.01);Hpa与nm23表达之间无明显相关性.结论 Hpa和nm23的表达与大肠癌的浸润转移密切相关.检测大肠癌组织中Hpa和nm23的表达水平对判断患者的预后和肝转移具有一定的参考价值.     

RNA干扰对肝素酶在肝癌细胞中表达及其肿瘤生长抑制的作用

目的:采用RNA干扰(RNAi)技术抑制肝癌细胞系HepG2肝素酶(Hpa)的表达,体内观察其对肿瘤生长的抑制作用.方法:以脂质体介导的方法将构建的2个抗Hpa短发卡式RNA(Hpa-shRNA)真核表达载体稳定转染人肝癌细胞株HepG2(高表达Hpa),并设空载体对照组;流式细胞技术鉴定转染稳定性,Western blot及RT-PCR检测肝素酶蛋白和mRNA表达;将干扰组和对照组的肿瘤细胞皮下接种裸鼠,比较各组肿瘤的体质量和体积大小,免疫组化法检测肝素酶在肿瘤组织的表达.结果:与空载体组相比,干扰后的HepG2细胞中Hpa mRNA和蛋白表达明显降低,干扰组裸鼠的肿瘤生长速度明显变慢,统计结果表明干扰组在抑制肿瘤生长方面与空载体组、空白对照组存在显著差异(P＜0.05),两干扰组无显著差异(P＞0.05).结论:应用RNAi技术沉默Hpa基因可以有效下调肝癌肿瘤细胞中Hpa mRNA及蛋白的表达,抑制体内肿瘤生长.     

Hpa、p53表达与大肠癌浸润转移的关系

目的 观察乙酰肝素酶（Hpa）及原癌基因053的表达变化与大肠癌浸润转移的关系。方法采用免疫组化EnVision法检测54例原发性大肠癌组织中的Hpa及p053蛋白。结果Hpa与大肠癌肿瘤直径有关（P〈0．01）,Hpa、p53与肿瘤浸润深度、淋巴结转移和肝转移有关（P均〈0．05）。结论Hpa和p53蛋白的表达在大肠癌浸润转移中起重要作用,联合检测Hpa和p53蛋白对判断大肠癌淋巴结和肝转移有一定参考价值。     

MTSS1和Hpa在结肠癌中的表达及其临床意义

目的研究转移抑制基因MTSS1及乙酰肝素酶Hpa在结肠癌组织中的表达水平,并探讨两者与结肠癌临床病理特征的关系。方法应用免疫组化EnVision法检测60例原发性结肠癌组织中MTSS1及Hpa蛋白的表达。结果 MTSS1在结肠癌组织中阳性表达率为18.3%(11/60),在肿瘤分期、有无淋巴结转移差异具有统计学意义(P<0.05),在结肠癌肿瘤不同组织学分型的表达差异无统计学意义(P>0.05);Hpa在结肠癌组织中阳性表达率为55.0%(33/60),与肿瘤分期、有无淋巴结转移比较差异有统计学意义(P<0.05),在结肠癌肿瘤不同组织学分型的表达差异无统计学意义(P>0.05)。结论 MTSS1及Hpa的表达与结肠癌的浸润、转移密切相关,联合检测MTSS1及Hpa的表达水平对判断结肠癌恶性程度和预后具有一定的参考价值。     

MAGE-A1的研究进展

MAGE-A1是一种肿瘤特异性抗原,很多研究表明它可以作为肿瘤免疫治疗的靶抗原.本文综述MAGE-A1在多种肿瘤中的表达及在肿瘤免疫治疗方面的应用前景     

肝癌组织中乙酰肝素酶与碱性成纤维细胞生长因子的表达及其与微血管密度的关系

乙酰肝素酶（Hpa）可降解细胞外基质（ECM）和基底膜中的硫酸乙酰肝素,破坏其完整性,同时释放在细胞外基质中储存的碱性成纤维细胞生长因子（b-FGF）,促进肿瘤血管的生成,从而有利于肿瘤细胞的侵袭和转移.现探讨Hpa、b-FGF与肝癌的临床病理特征及肿瘤血管新生之间的关系.     

肝素酶与胃癌侵袭转移的关系

胃癌是人类最常见的恶性肿瘤之一,位居消化道肿瘤之首。多年来,胃癌的发生发展及其侵袭转移一直是众多学者研究的热点。肝素酶(Hpa)是一类βD糖苷内切酶(endoβDglucuronidase),它既可存在于正常组织,也表达于多种恶性肿瘤细胞表面,以水解的方式降解组织屏障中的糖蛋白成分硫酸乙酰肝素糖蛋白(HSPGs),释放HSPGs结合的生物活性分子,促进细胞的侵袭和转移,诱导肿瘤血管生成,降低肿瘤患者的生存率。此文就Hpa与胃癌侵袭转移的关系作一综述。     

乙酰肝素酶在大肠癌中的表达及意义

目的探讨大肠癌组织中乙酰肝素酶（Hpa）的表达,及其与大肠癌发生、浸润、转移及预后的关系。方法采用免疫组化Envison法测定大肠癌的癌组织与正常大肠组织中Hpa蛋白表达,结合大肠癌的临床病理学特征及预后进行分析。结果 Hpa蛋白在大肠癌组织中的表达高于正常组织（P〈0.01）。Hpa蛋白在大肠癌无淋巴结转移者的表达低于有淋巴结转移者,Ⅰ、Ⅱ期低于Ⅲ、Ⅳ期,生存时间小于5 a者低于高于5 a者（P均〈0.01）。结论 Hpa在大肠癌组织中高表达,其可能在大肠癌的发生、侵袭和转移中起作用。     

肝癌细胞株中肿瘤特异性抗原MAGE、GAGE、BAGE基因表达的研究

目的 研究MAGE、GAGE、BAGE基因在肝癌细胞株中的表达情况,评价这些肿瘤特异性抗原作为肿瘤分子标记以及肿瘤免疫治疗特异性靶位的可能性。 方法 用RT-PCR检测国内建株的肝癌细胞株SMMC-7721、QQY-7701、BEL-7402中MAGE-1、MAGE-3、GAGE1-8、GAGE1-2和BAGE基因mRNA的表达,以GAPDH基因作为检测内对照,并与非肿瘤肝穿组织比较。 结果 肝癌细胞株SMMC-7721表达MAGE-1和BAGE基因;QQY-7701表达MAGE-3和BAGE基因;BEL-7402表达MAGE-1和GAGE1-2基因;3株肝癌细胞至少表达其中一个基因。肝硬化病人肝穿刺组织中MAGE、GAGE、BAGE基因表达均为阴性。 结论 MAGE、GAGE、B A G E肿瘤特异性抗原可以作为肝癌早期诊断的分子标记,并具有作为肝癌免疫治疗特异性靶位的潜在价值。     

胃癌患者外周血微转移指标的检测及意义

目的 研究癌胚抗原(CEA)、肝素酶(Hpa)、多巴脱羧酶(DDC)、基质金属蛋白酶7(MMP-7)、磷酸丝氨酸磷脂酶(PSPH)与胃癌早期转移之间的关系.方法 利用RT-PCR检测方法对80例胃癌患者及16例胃良性病变患者及20例健康体检者的血清进行检测,检测胃癌患者外周血中CEA nRNA、Hpa mRNA、DDCmRNA、MMP-7 mRNA、PSPH mRNA的表达.结果 胃癌组较胃良性瘤组及健康组外周血CEAmRNA、Hpa mRNA、DDC mRNA、MMP-7mRNA、PSPH mRNA表达阳性率明显增高(P＜0.05)；在胃癌组中,五种标志物阳性率中CEAmRNA最高,差异有统计学意义(P＜0.05)；胃癌组CEA mRNA、Hpa mRNA、DDC mRNA、MMP-7 mRNA、PSPH mRNA这五种指标在肿瘤部位、临床分期、组织学类型、pTNM、淋巴结转移及肝转移间阳性率比较均有统计学差异(P＜0.05)；随着临床分期的增加,这些肿瘤标志物的表达率也明显升高.结论 胃癌患者外周血中存在CEAmRNA、Hpa mRNA、DDCmRNA、MMP-7mRNA、PSPH mRNA表达,CEA mRNA阳性率最高,该五项指标能预测外周血微转移和判断预后.     

Research and Evaluation of the Influence of the Construction of the Gate and the Influence of the Piston Velocity on the Distribution of Gases into the Volume of the Casting

Distribution of gasses to the cast volume and volume of pores can be maintained within the acceptable limits by means of correct setting of technological parameters of casting and by selection of suitable structure and gating system arrangement. The main idea of this paper solves the issue of suitability of die casting adjustment—i.e., change of technological parameters or change of structural solution of the gating system—with regards to inner soundness of casts produced in die casting process. Parameters which were compared included height of a gate and velocity of a piston. The melt velocity in the gate was used as a correlating factor between the gate height and piston velocity. The evaluated parameter was gas entrapment in the cast at the end of the filling phase of die casting cycle and at the same time percentage of porosity in the samples taken from the main runner. On the basis of the performed experiments it was proved that the change of technological parameters, particularly of pressing velocity of the piston, directly influences distribution of gasses to the cast volume.     

Weighing the QT Intervals with the Slope or the Amplitude of the T Wave

Objective: The reproducibility of QT interval measurements is low, even for the mean QT interval based on the standard ECG. In this study we analyzed whether the reproducibility of the mean weighed QT interval was better than the simple mean QT interval. The weighing was based on the amplitude of the T wave or the slope of the steepest tangent on the terminal part of the T wave. Material and methods: 12‐lead ECGs of 130 postmyocardial infarction patients were obtained. The QT intervals were measured by the tangent‐method on two occasions by the same observer Mismatch QT intervals were defined as QT intervals that were measured at only one occasion. Sixteen ECGs were rejected. The data were split into 34 and 80 ECGs for optimization and validation of the weighing, respectively. The weighed QT dispersion was calculated as the weighed mean of the three longest minus the weighed mean of the three shortest QT intervals. Results: Weighing with the slope increased the reproducibility by 41% (P = 3 10^‐6), but weighing with the amplitude reduced it by 20% (P = 0.02). However, if measurements with errors above 75 ms were rejected, weighing with the slope or the amplitude increased the reproducibility with 26% and 20% (P = 0.02), respectively. Weighing did not change the reproducibility of the weighed QT dispersion. Conclusion: Weighing with the slope improved the reproducibility of the mean weighed QT interval. However, if measurements with errors above 75 ms were rejected, weighing with the amplitude also increased the reproducibility. Weighing did not change the reproducibility of the weighed QT dispersion. Weighing is particularly efficient at reducing the negative impact of mismatch QT intervals on the reproducibility. A.N.E. 2002;7(1):4–9     

县级以下综合医疗机构医务人员对现代结控知识的认识现状调查

目的本文旨在了解医务人员现代结控知识掌握的现状及培训效果?方法于培训前后进行问卷调查,内容包括:病例发现?结核病诊断及化疗?结果培训前疫情报告和转诊,回答正确者占75.2%?71.7%;对临床表现?查痰和诊断依据,回答正确者占83.5%?42.5%?40.8%;抗痨药物?用药方法?化疗原则?短化方案?短化疗程?治愈标准六项,回答正确者占58%?14.4%?20.8%?9.2%?17%?24.3%?培训后再次调查发现,90%以上医务人员对现代结控基本知识已掌握?结论各级医务人员现代结控知识是很贫乏的,因此,对其进行系统培训是极为必要的,此项工作省时?省力?投入少,可收到事半功倍的效果。     

大骨节病病区水,粮实验对动物的骨和软骨中化学元素组成的影响

用质子激发 X 线荧光分析方法(PIXE)测定了大骨节病病区和非病区的水、粮以及用该水粮喂养的大白鼠的肋软骨和硬骨中22种化学元素的含量。结果发现水粮中存在差异的元素反应在用该水粮喂养的大白鼠的骨、软骨中也存在差异,含量都低的元素有 P、Mn、Cu、As、Zn。都高的有铅。其中锌低在水、粮、硬骨和软骨中都完全一致呈非常显著性差异(p<0.01)。提示病区水、粮中化学元素对骨质的影响不是单一元素缺乏或过多所致,而是多种元素的复合因素。     

Assessment of the Soft-Tissue Seal at the Interface between the Base of the Fixed Denture Pontic and the Oral Mucosa

Fixed dentures (bridges) are often selected as a treatment option for a defective prosthesis. In this study, we assess the contact condition between the base of the pontic and oral mucosa, and examine the effect of prosthetic preparation and material biocompatibility. The molars were removed and replaced with experimental implants with a free-end type bridge superstructure after one week. In Experiment 1, we assessed different types of prosthetic pre-treatment: (1) the untreated control group (Con: mucosa recovering from the tooth extraction); (2) the laser irradiation group (Las: mucosa recovering after the damage caused by a CO₂ laser); and (3) the tooth extraction group (Ext: mucosa recovering immediately after the teeth extraction). In Experiment 2, five materials (titanium, zirconia, porcelain, gold-platinum alloy, and self-curing resin) were placed at the base of the bridge pontic. Four weeks after the placement of the bridge, the mucosa adjacent to the pontic base was histologically analyzed. In Experiment 1, the Con and Las groups exhibited no formation of an epithelial sealing structure on the pontic base. In the Ext group, adherent epithelium was observed. In Experiment 2, the sealing properties at the pontic interface were superior for titanium and the zirconia compared with those made of porcelain or gold-platinum alloy. In the resin group, a clear delay in epithelial healing was observed.     

高胆红素血症对肿瘤标志物测定的影响探讨

目的探讨高胆红素血症对Ca19-9、Ca24-2和CEA检测的影响.方法对320例胆管、胆囊良恶性疾病病人,15例胆囊炎病人的胆汁和血清以及10例肝硬化、10例黄疸肝炎病人进行Ca19-9、Ca24-2和CEA检测.结果在良性胆管、胆囊疾病中,Ca19-9的假阳性最高;在胆红素增高的良性疾病中,Ca19-9假阳性率达46.7%;15例胆汁和血清以及10例肝硬化和10例黄疸肝炎病人中,Ca19-9的假阳性率分别为93%、20%、80%和80%.结论高胆红素血症对Ca19-9检测影响最明显,胆囊、胆管良恶性疾病鉴别时,以Ca24-2和CEA检测为佳.     

Echocardiography in the Emergency Department: From the Perspective of the Emergency Physician and the Cardiologist

Echocardiography performed in the emergency department must adapt to this new setting for noninvasive diagnostic testing. Emergency physicians require echocardiography to provide rapid diagnosis in life-threatening emergencies. New initiatives are being proposed by emergency physicians in the delivery of this test. Cardiologists now use echocardiography in the emergency-department to make the diagnosis of heart disease earlier and with greater accuracy.