Isolation and characterization of endometrial leukocyte populations by flow cytometry

Potentialities of the flow cytornetry method in studies of the qualitative and quantitative composition of endometrial immunocompetent cells are demonstrated. The studied leukocyte populations are found to occur in negligible quantities in the endometrium. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 1, pp. 68–71, January, 1994.     

Formation of T lymphocytes inactivating allogeneic stem cells in the course of macrophage-thymocyte interaction

Allogeneic stem cells interact with T lymphocytes which are formed when intact thymocytes are cultured with syngeneic mononuclear phagocytes. The capacity of these T cells to inhibit the colony-stimulating activity of stem elements in endo- and exocolony formation tests is demonstrated. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 3, pp. 298–300, March, 1996 Presented by N. N. Trapeznikov, Member of the Russian Academy of Medical Sciences     

Inhibition of IL-2-Dependent proliferation of human lymphocytes and of the expression of interleukin-2 receptors by synthetic peptide fragments of α-2 interferon

It is shown that biologically active peptides inhibit the concanavalin A-induced generation of cells which may proliferate in response to exogenous recombinant interleukin-2. Determination of the number of CD25-containing cells showed that synthetic peptides are able to lower not only the number of CD25-containing cells but the level of its expression as well. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 1, pp. 65–68, January, 1994 Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences     

Involvement of fibronectin and interacting serum components in the regulation of activity of human natural killer cells

The cytotoxic activity of natural killer cells and the intensity of conjugate formation are studiedin vitro in the natural cytotoxicity reaction against³H-uridine-labeled human erythromyeloleukotic cells K-562 in the presence of fibronectin, γ-globulin, and fibronectin/γ-globulin combination. It is demonstrated that fibronectin does not change natural cytotoxicity, γ-globulin increases the activity of human natural killer cells, and the fibronectin — γ-globulin combination increases both the intensity of conjugate formation and the cytotoxic activity of natural killer cells. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 7, pp. 54–59, July, 1994     

Fibronectin as a compensating factor in the natural cytotoxicity system in interferon-dependent immunodeficiency

The cytotoxicity of natural killer cells (NK) against³H-uridine-labeled target cells (TC, human erythromyeloleukosis cells K-562) and the intensity of conjugate formation in the NK:TC system in the presence of γ-interferon, C-reactive protein, and human fibronectin are studiedin vitro in 14 patients with multiple sclerosis. It is shown that γ-interferon and C-reactive protein decrease the cytotoxic activity of NK with a simultaneous stimulation of conjugate formation in the NK:TC system. The correlation between the studied parameters becomes weaker. Human fibronectin induces collateral changes in the activity of NK and in the number of effector:target conjugates formed in the natural cytotoxicity reaction. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 7, pp. 67–70, July, 1994     

Accessory groups of nonapeptidergic neurosecretory cells of the hypothalamus and adjacent regions of the brain in rats under conditions of dehydration

Study of the hypothalamus and adjacent areas of the brain in adult rats revealed that, along with the osmosensitivity of neurosecretory cells, the transport of vasopressin and oxytocin along their axons and their release from the terminals into the bloodstream are impeded. This is due to the absence of axonal bonds between the accessory groups of neurosecretory cells and the posterior lobe of the pituitary. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 3 pp. 268–270, March, 1996     

Determination of the biological activity of chalone-containing preparation from Ehrlich ascitic carcinoma and its fractions obtained by high-performance liquid chromatography in a cell culture derived from this tumor

It is shown that cultured Ehrlich ascitic carcinoma cells are a convenient test system for the investigation of the effects of various factors on DNA synthesis in the cells of this tumor. The application of this system markedly facilitates fractionation of a chalone-containing preparation, the purpose of this fractionation being the isolation of components affecting specific phases of the mitotic cycle. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 1, pp. 80–82, January, 1994     

Antitumor activity of normal bone marrow cells

After separation of normal murine bone marrow cells in a Percoll density gradient cellular fractions with densities of 1.076 and 1.060 g/ml are capable of suppressing thein vitro growth of leukemia cells. The cytostatic activity of these fractions, however, does not surpass the level of antitumor antiproliferative activity intrinsic to intact bone marrow cells. These cells were found to be capable of joining the splenocytes, thymocytes, and lymph node cells in effector cytostatic cooperation and thus enhance the final antitumor effect. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 8, pp. 181–183, August, 1995     

Paneth cells of the eastern gray squirrel (Sciurus carolinensis) and red-cheeked suslik (Citellus erythrogenys brandt)

It is shown that the structure of Paneth cells in duodenum of the eastern gray squirrel and red-cheeked suslik is similar to that in other mammals. Seasonal differences in Paneth cell structure are not found in squirrels, while in susliks they are marked. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 2, pp. 210–213, February, 1996 Presented by V. A. Trufakin, Member of the Russian Academy of Medical Sciences     

Morphofunctional changes in the intima of collateral arteries in modeled aorta coarctation

In modeled aorta coarctation long-term functioning of collateral arteries is shown to be accompanied by activation of subintimal smooth muscle cells and elastofibrosis of the intima, which are a manifestation of hemodynamic arteriosclerosis. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 7, pp. 82–85, July, 1994 Presented by L. D. Sidorova, Member of the Russian Academy of Medical Sciences     

Effect of autologous sera on mitogenic stimulation of peripheral blood lymphocytes of mini-pigs chronically intoxicated with alcohol

There are no statistically significant differences in the stimulation index for purified lymphocytes of control mini-pigs and mini-pigs with chronic alcohol intoxication. Autologous sera of control and experimental animals strongly suppress mitogen-induced blast transformation of lymphocytes without death of these cells. There are no statistically significant intergroup differences in the absolute number (per mm³) of T lymphocytes in peripheral blood. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 7, pp. 89–91, July, 1994 Presented by N. K. Permyakov, Member of the Russian Academy of Medical Sciences     

Specific morphofunctional features of the parietal cells of hibernating rodents in different physiological states

Low pH values of the stomach contents and activation of the parietal cells are revealed during the spontaneous awakening-associated normothermia of red-cheek sousliks. These factors help maintain the acid-base balance in the organism. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 7, pp. 103–106, July, 1995 Presented by V. P. Kaznacheev, Member of the Russian Academy of Medical Sciences     

Metastasizing of human melanoma on immunodeficient mice. Tumor cells in the circulation

The subline Bro of human melanoma xenograft with a high metastasizing activity is studied. The data obtained by flow fluorometry after staining with tumor-specific monoclonal antibodies indicate that free melanoma cells are present in the peripheral blood of animals; the largest number of these cells is found in mice with combined immunodeficiencies (beige/nude). Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 8, pp. 188–189, August, 1994 Presented by Yu. A. Romanov, Member of the Russian Academy of Medical Sciences     

Comparative analysis of progesterone and estradiol reception in human myoma

Cytosolic and plasma membrane receptors for progesterone and estradiol are studied in myomatous nodes (MN) and in histologically unaltered myometrium (HUM) against the background of myoma. The level of cytosolic receptors for both hormones is higher in the myoma cells than in the essentially healthy myometrium. In the plasma membranes the progresterone reception is reduced and the estradiol reception is unchanged compared with HUM. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 7, pp. 33–34, July, 1994     

The murine toxin ofYersinia pestis: Some enzymatic activities

The phosphatase, phosphodiesterase, and phospholipase activities identified in this study in a highly purified preparation of theYersinia pestis murine toxin should shed light on the mechanism by which this toxin acts on the target cells of plague-sensitive animals Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 12, pp. 602–605, December, 1995 Presented by D. S. Sarkisov, Member of the Russian Academy of Medical Sciences     

Preparation and characterization of murine monoclonal antibodies toSalmonella typhimurium K-antigen

Monoclonal antibodies toS. typhimurium K-antigens representing IgG1 immunoglobulins with a “kappa”-type light chain are prepared and characterized. They are highly active in indirect enzyme immunoassay with purified K-antigens and whole fixedS. typhimurium cells and virtually do not cross-react with O-antigen of the same bacterial species or withE. coli antigenic structures. The kinetics of binding and ability to agglutinate whole bacterial cells are studied and epitope analysis is carried out, which shows that antigenic determinants ofS. typhimurium K-antigen qualitatively differ from those of O-antigen of the same bacterium. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 6, pp. 633–635, June, 1994 Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences     

Stimulation of proliferative activity of human natural killers (CD16+CD56+ cells) by recombinant interleukin-3in vitro

The proliferative activity of human natural killers (CD16⁺CD56⁺ cells) in the presence of 100 and 1000 IU/ml human recombinant interleukin-3 is investigatedin vitro. It is shown that recombinant interleukin-3 reliably enhances natural killer proliferation, causing a 9–15.2-fold increase of³H-thymidine uptake by CD16⁺CD56⁺ cells both in complete culture medium and in conditioned medium. The effect of the factor is 3.9–6.4 and 3.6–8.9-fold more potent than that of recombinant interleukin-2 and granulocyte-macrophage colony-stimulating factor, respectively, in the same doses. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 4, pp. 409–412, April, 1995 Presented by S. V. Prozorovskii, Member of the Russian Academy of Medical Sciences     

Decrease of antitumor and suppressor activities of nonadherent bone marrow cells by culturing in medium conditioned with ehrlich adenocarcinoma cells

It was demonstrated that proliferation of bone marrow cells cultured in medium conditioned with tumor cells is somewhat increased. A much more potent stimulation of bone marrow cell proliferation is observed after the removal of cells carrying the erythroblast antigen. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. N ^o 5, pp. 514–517, May, 1994 Presented by N. V. Vasil'ev, Member of the Russian Academy of Medical Sciences     

Informational capacity of the rat sensorimotor cortex in the postresuscitation period (morphometric analysis of a neuronal population)

The number of neurons was counted in different functional neuronal complexes of the sensorimotor cortex in albino rats in the control and at different times after clinical death caused by asphyxia. A decrease of the number of neurons from 2.5×10⁶ in the control to 1.56×10⁶ toward the 30th day of the postresuscitation period was found in the sensorimotor cortex. The complexes of small neurons in the upper floor (layers II–IV) suffer badly, while the neurons of layer VI are damaged to a somewhat lesser degree and the cells of layer V are minimally altered. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 3, pp. 331–333, March, 1995 Presented by L. V. Poluektov, Member of the Russian Academy of Medical Sciences     

A study of nonpathogenicFrancisella,Brucella, andYersinia strains as producers of recombinant β-endorphin

Yersinia pseudotuberculosis, Brucella abortus, andFrancisella tularensis strains producing a recombinant β-endorphin have been obtained. The highest production of this peptide, which displays physiological activity, was recorded for cells of theY. pseudotuberculosis strain 2243 (pSK95E). Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 6, pp. 612–615, June, 1994