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1.
O. Ogueh G. Khastgir J. Studd J. Jones J. Alaghband-Zadeh M. R. Johnson 《Calcified tissue international》1999,65(3):211-213
There is evidence that infants of insulin-dependent diabetics have increased intrauterine bone resorption and reduced bone
mineral content at birth. The aim of this study was to determine if type I diabetes is associated with abnormal maternal bone
metabolism. We measured the circulating levels of carboxyterminal propeptide of type I procollagen (PICP) and cross-linked
carboxyterminal telopeptide of type I collagen (ICTP) in the third trimester of pregnancy in samples obtained from 19 pregnant
women with type I diabetes and 19 pregnant controls, to monitor the rate of bone formation and degradation, respectively.
Diabetic control was considered to be good as the mean hemoglobin A1 level was less than 8.5%. The circulating levels of PICP were significantly higher in pregnant women with insulin-dependent
diabetes than in controls with uncomplicated pregnancy (median IDDM 147 μg/liter, control 115 μg/liter, P= 0.0014), but there was no significant difference in the circulating levels of ICTP between the two groups (median IDDM 4.6
μg/liter, control 4.6 μg/liter, P= 0.907). Therefore, our findings suggest that there is an increase in bone formation in pregnant women with type I diabetes
which may be related to the increased amount of insulin administered and the improvement in diabetic control associated with
pregnancy.
Received: 26 August 1998 / Accepted: 12 March 1999 相似文献
2.
N. Yoshimura T. Hashimoto K. Sakata S. Morioka T. Kasamatsu C. Cooper 《Calcified tissue international》1999,65(3):198-202
The purpose of this study was to ascertain whether biochemical markers of bone turnover predict bone loss. The survey was
carried out in Taiji, Wakayama Prefecture, Japan. From a list of inhabitants aged 40–79 years, 400 participants (50 men and
50 women in each of four age groups) were selected randomly. Bone mineral density (BMD) was measured, and blood and urine
samples of all participants were examined to obtain values for eight biochemical markers: alkaline phosphatase (ALP), bone
Gla protein (BGP), type I procollagen (carboxyterminal peptide of type I procollagen; PICP), cross-linked carboxyterminal
telopeptide region of type I collagen (ICTP), and urinary excretion of calcium (Ca), phosphate (P), pyridinoline (Pyr), and
deoxypyridinoline (D-Pyr). Each marker was evaluated as a predictor of the rate of bone change in lumbar spine and femoral
neck BMD over a 3-year period. The value of Pyr was significantly related to the change of lumbar spine BMD in men (P= 0.009), and that of BGP was found to be significant in women (P= 0.045). By contrast, none of the bone markers significantly correlated with bone loss at the femoral neck. The coefficient
of determination at the lumbar spine was 5% and 7% at the femoral neck only. We conclude that biochemical markers of bone
turnover cannot predict bone loss rates in middle-aged or elderly Japanese men and women over a 3-year period with sufficient
accuracy for use in clinical decision making.
Received: 26 January 1998 / Accepted: 9 July 1998 相似文献
3.
Bone Mineral Density and Biochemical Markers of Bone Turnover in Peri- and Postmenopausal Women 总被引:2,自引:0,他引:2
De Leo V Ditto A la Marca A Lanzetta D Massafra C Morgante G 《Calcified tissue international》2000,66(4):263-267
Bone mineral density (BMD) measured by densitometry is the elective parameter for the diagnosis of osteopenia and osteoporosis.
Biochemical markers have been proposed as sensitive indicators of high bone turnover and for monitoring response to antiresorptive
treatment. We conducted a retrospective study to investigate the values of biochemical markers of bone metabolism with a view
to early diagnosis of osteoporosis and monitoring of hormone replacement and calcitonin therapy. The subjects were 415 women,
mean age 51 ± 8 years (43–62 years) in peri- and postmenopause, recruited at the Menopause Center of Obstetrics and Gynecology
Department of Siena University and divided in five groups. Bone densitometry was performed in all subjects and blood samples
were taken for assayed biochemical markers, that is, [osteocalcin (OC), parathyroid hormone (PTH), type 1 procollagen (PICP),
and calcitonin (CT)].
Three groups of women were divided into two subgroups: those with normal and those with low BMD (<1 SD). Basal concentrations
of PCP1, OC, PTH, and CT were compared in the various groups. Two groups of postmenopausal women with BMD below the normal
were treated with estrogen replacement therapy and unmodified eel calcitonin.
We evaluated whether some of these biochemical markers of bone turnover could help identify women with low BMD and whether
they could be useful for monitoring the results of antiresorptive therapies.
Markers of bone formation (PICP and OC) make it possible to distinguish women with high turnover who are at risk for osteoporosis
from women with low turnover in menopause. A good correlation was also found between changes in levels of these markers and
changes in BMD during treatments, which suggests that the PICP and OC would be useful for monitoring response to antiresorptive
therapy.
Received: 29 March 1998 / Accepted: 2 November 1999 相似文献
4.
R. A. Faulkner D. A. Bailey D. T. Drinkwater H. A. McKay C. Arnold A. A. Wilkinson 《Calcified tissue international》1996,59(5):344-351
Normative bone mineral density (BMD) and bone mineral content (BMC) values for the total body (TB), proximal femur (PF),
and antero-posterior lumbar spine (LS) were obtained from a large cross-sectional sample of children and adolescents who were
8–17 years of age. There were 977 scans for the TB, 892 for the PF, and 666 for the LS; bone mineral values were obtained
using a HOLOGIC QDR 2000 in array mode. Data are presented for the subregions of the PF (femoral neck, trochanter, intertrochanter,
and the total region) and for the LS (L1–L4 and L3). Female and male values for the FN, LS (L1–L4), and the TB were compared
across age groups using a two-way ANOVA. In addition, we compared the 17-year-old female values to a separate sample of young
adult women (age 21). At all these sites, BMC and BMD increased significantly with age. There was no gender difference in
TB BMC until age 14 or in TB BMD until age 16, when male values were significantly greater. Females had significantly greater
LS BMC at ages 12 and 13, but by age 17 the male values were significantly greater. Females had significantly greater LS BMD
across all age groups, however. Males had significantly greater FN BMC and BMD across all age groups. There were no significant
differences in BMC or BMD at any sites between the 17- and 21-year-old women.
Received: 29 September 1995 / Accepted: 1 April 1996 相似文献
5.
E. Jódar Gimeno M. Muñoz-Torres F. Escobar-Jiménez M. Quesada Charneco J. D. Luna del Castillo N. Oleà 《Calcified tissue international》1997,61(5):370-376
Active hyperthyroidism is associated with reduced bone mass. Nevertheless, not all patients show the same risk for developing
osteoporosis. Our aim was to analyze some clinical and biochemical potential predictors of low bone mass in hyperthyroid patients.
We studied 127 consecutive hyperthyroid patients (110 females, 17 males; aged 42 ± 16 years). Bone mineral density (BMD) was
measured by dual X-ray absorptiometry (DXA) at lumbar spine (LS; L2–L4) and femoral neck (FN). Data were expressed as g/cm2 and T-score. Patients were placed into two groups based on recent WHO criteria: Group A, no osteoporosis (n = 98); and group
B, lumbar or femoral osteoporosis (n = 29). Study protocol included evaluation of osteoporosis risk factors, anthropometrical
variables, thyroid function, and bone turnover markers. Receiver-operating characteristic (ROC) plots for the precision of
bone markers and multivariate analysis for the prediction of BMD and osteoporosis were performed. Group B showed greater age
and proportion of menopausal females; lower weight, height, and calcium intake; longer duration of menopause; and greater
levels of total and bone alkaline phosphatase and of urine hydroxyproline. No differences in thyroid function, osteocalcin,
tartrate-resistant acid phosphatase, and type I collagen C-telopeptide (ICTP) were found. The best predictive model accounted
for 46% and 62% of the variability of lumbar and femoral BMD respectively and correctly classified 89% of the osteoporotic
hyperthyroid patients. No significant difference in ROC plots was observed. It is concluded that hyperthyroid patients with
lumbar or femoral osteoporosis show a typical clinical and biochemical profile illustrating that the relationship between
BMD and bone markers is better in high turnover states. Classical bone turnover markers show high performance in the evaluation
of hyperthyroid bone disease.
Received: 5 May 1997 / Accepted: 5 June 1997 相似文献
6.
R. Dresner-Pollak R. A. Parker M. Poku J. Thompson M. J. Seibel S. L. Greenspan 《Calcified tissue international》1996,59(5):328-333
Although over 90% of hip fractures occur in patients over age 70, few data are available on femoral bone loss in this age
group. To examine the relationship between biochemical markers of bone turnover and femoral bone loss in the elderly, 36 female
and 17 male, healthy, community-dwelling elderly over age 65 (mean ± SD age: women 71 ± 4 years, men 75 ± 5 years) were followed
for 3 years. Annual bone mineral density measurements of the hip and lumbar spine by dual-energy x-ray absorptiometry (DXA)
were obtained and biochemical markers of bone resorption (urinary N-telopeptide crosslinks, free pyridinoline, total pyridinoline,
total deoxypyridinoline, and hydroxyproline) and bone formation (serum osteocalcin, bone-specific alkaline phosphatase) were
obtained at the end of year 3. In elderly women, longitudinal bone loss at the total hip was negatively correlated with markers
of bone resorption (r =−0.39 to −0.52, P < 0.05), bone formation (r =−0.38, P < 0.05), and age (r =−0.39, P < 0.05). Markers of bone resorption were correlated with markers of bone formation (r = 0.63 to 0.74, P < 0.01). In multiple regression analysis, urinary N-telopeptide crosslinks (marker of resorption), serum osteocalcin (marker
of formation), and serum parathyroid hormone explained 43% of the variability of bone loss at the total hip in women. These
parameters were not related to bone loss in men. We conclude that femoral bone loss increases with age in women over 65. Measurements
of specific biochemical markers of bone turnover are correlated with longitudinal bone loss in elderly women. These markers
may help identify women at greatest risk for bone loss who would benefit most from therapeutic interventions.
Received: 28 January 1996 / Accepted: 3 May 1996 相似文献
7.
It is unclear whether both bone resorption and formation are affected by glycemic control, and contribute to diabetic osteopenia.
In this study, 20 patients with noninsulin-dependent diabetes mellitus (12 men and 8 postmenopausal women) and 20 healthy
control subjects (10 men and 10 postmenopausal women) were examined at baseline and 2 months. The diabetic patients showed
an improvement of glycemic control (decreased HbA1c) at the second measurement. Analysis of variance showed that there was no effect of gender on the variables that increased
with improved glycemic control, and therefore results are presented for both male and female subjects. Baseline values of
serum osteocalcin, a marker of formation, were significantly lower in diabetic patients compared with healthy subjects (2.5
± 1.3 versus 4.4 ± 1.4 ng/ml; P= 0.0006), but markers of bone resorption [urinary pyridinoline (PYD), deoxypyridinoline (DPD)] did not differ. Improved glycemic
control in diabetic patients resulted in increased values of PYD (P= 0.012), DPD (P= 0.049), serum osteocalcin (P= 0.001), and serum insulin-like growth factor I (IGF-I, P= 0.003), but no change in serum parathyroid hormone or 25-hydroxyvitamin D. In diabetic patients there were inverse correlations
for the percent change from baseline to improved glycemic control for osteocalcin and HbA1c (r =−0.53; P= 0.016) and glucose (r =−0.46; P= 0.050). These data suggest that improved glycemic control is accompanied by an increase in bone turnover for male and female
diabetic patients, possibly mediated by increased levels of circulating IGF-I.
Received: 8 August 1997 / Accepted: 20 January 1998 相似文献
8.
S. Mora C. Prinster M. C. Proverbio A. Bellini S. C. L. de Poli G. Weber G. Abbiati G. Chiumello 《Calcified tissue international》1998,63(5):369-374
During growth, bones change their dimensions rapidly with the changes involving both formation and resorption processes.
Small cross-linked peptides coming from type I collagen molecules are excreted in urine when bone is resorbed. To date, conflicting
results have been presented concerning the age- and puberty-related changes of urinary markers. The purpose of the present
study was to verify the effect of age, gender, and puberty on the urinary excretion of type I collagen degradation products
in healthy children and adolescents. Timed spot urines from 176 children (4–20 years old) and 50 young adults were analyzed.
The concentrations of N-telopeptides of type I collagen (NTx), pyridinolines (Pyr), and deoxypyridinolines (Dpyr) were measured,
and the results were normalized to creatinine. Age-related changes in cross-links excretion were observed. The levels decreased
with age, and a peak of excretion was shown at the beginning of adolescence. Prepubertal levels of all the markers were four-
to five-fold higher than in adults, and they decreased towards adult levels in late puberty. Girls had significantly higher
levels of all biochemical markers than boys at pubertal stage 2. We also observed a remarkable effect of puberty on the levels
of bone degradation products that was independent of age and gender. Our results indicate that bone resorption is high in
children relative to that in adults, and that urinary levels of NTx, Pyr, and Dpyr change as a function of age, gender, and
puberty.
Received: 30 October 1997 / Accepted: 20 February 1998 相似文献
9.
S. Herrero O. M. Calvo C. García-Moreno E. Martín J. I. San Román M. Martín J. R. García-Talavera J. J. Calvo J. del Pino-Montes 《Calcified tissue international》1998,62(3):260-265
Diabetes and estrogen deficit are known causes of osteopenia, diabetes being associated with a low bone turnover and estrogen
deficit with a high bone turnover. In the present work, we studied the effect of combined ovariectomy and diabetes on bone
mineral content (BMC) and bone mineral density (BMD) and several bone markers in the rat. Four groups of rats were studied:
control (C), ovariectomized (O), diabetic (D), and ovariectomized and diabetic (DO). Twelve weeks after starting the experiments,
BMC and BMD of the first six lumbar vertebrae were measured; a bone formation marker (BGP) and a bone resorption marker (free
collagen cross-links, PYD) were also analyzed. Diabetic rats showed diminished gain in bone mass, BMC (D: 0.417 ± 0.028 g,
DO: 0.422 ± 0.020 g) and BMDs (D: 0.171 ± 0.006 g/cm2, DO: 0.174 ± 0.006 g/cm2) both being significantly (P < 0.001) lower than those of control (C: BMC 0.727 ± 0.024 g and BMD 0.258 ± 0.004 g/cm2) and ovariectomized (O: BMC 0.640 ± 0.044 g and BMD 0.240 ± 0.009 g/cm2) groups. Moreover, the BMC and BMD of the C group were significantly (P < 0.05) higher than that of the O group. BGP and PYD levels were significantly (P < 0.01) higher in the O group (BGP: 138.2 ± 16.8 ng/ml, PYD: 270.2 ± 17.8 nM/mM) than those found in the control rats (BGP:
44.7 ± 4.8 ng/ml, PYD: 165.6 ± 12.5 nM/mM); the D group showed significantly (P < 0.01) lower values (BGP: 27.4 ± 14.6 ng/ml, PYD: 55.0 ± 7.4 nM/mM) than those of the control group. The DO group showed
similar levels (BGP: 43.4 ± 5.1 ng/ml, PYD: 146.7 ± 14.6 nM/mM) to those found in the C group. Although bone marker levels
in the O and D groups were in accordance with those expected in these situations, in the DO group the corresponding levels
are apparently ``normal.' Also, the decrease of gain in bone mass observed after combining estrogen deficit and diabetes
(DO group) did not seem to be more marked than that caused by diabetes alone.
Received: 7 January 1997 / Accepted: 7 August 1997 相似文献
10.
S. Aota T. Nakamura K. Suzuki Y. Tanaka Y. Okazaki Y. Segawa M. Miura S. Kikuchi 《Calcified tissue international》1996,59(5):385-391
We examined the bone turnover and bone mass in adjuvant-induced arthritis in rats and assessed the effects of indomethacin
in this model. One hundred ten SD rats, 6 weeks of age, were assigned to 11 groups and injected with adjuvant or solvent in
the right foot. Adjuvant-injected rats were orally administered indomethacin at doses of 0 (vehicle), 0.1 (low), 0.5 (medium),
and 1.5 (high) mg/kg body weight from the start (day 0). Animals were sacrificed on days 0, 14 (acute phase), and 28 (chronic
phase). In the arthritic-control group, serum osteocalcin level and bone mineral content of the fourth lumbar body (L4) and
the femur were significantly reduced on day 14. Serum alkaline-phosphatase was increased on day 28. Trabecular bone volume
of L4 was decreased on day 14, and the value was further decreased on day 28. Bone formation rate (BFR/BS) was significantly
reduced on day 14, and then osteoclast number (Oc.N/BS) increased on day 28. Indomethacin treatment dose-dependently prevented
increases in paw volume and osteoclast number. In the high dose group, these indices were maintained at the same level with
those in the normal group. However, indomethacin treatments were not able to maintain the parameters of bone formation such
as serum osteocalcin and BFR/BS values, and the trabecular bone mass decrease was only partially prevented. These data clearly
indicated both reduced bone formation and increased bone resorption as the causes of bone loss in adjuvant-induced arthritis
in rats. Increased bone resorption seemed to be due to the increased activity of prostaglandins, but bone formation defect
would be related to other factors in this animal model.
Received: 13 January 1996 / Accepted: 3 May 1996 相似文献
11.
C. Cepollaro S. Gonnelli C. Pondrelli A. Montagnani S. Martini D. Bruni C. Gennari 《Calcified tissue international》1999,65(2):129-132
We studied 21 patients (11 men and 10 women) with osteogenesis imperfecta (OI) and 21 age- and sex-matched controls. In all
patients we measured serum levels of total alkaline phosphatase (ALP), type I procollagen carboxy-terminal propeptide (PICP),
osteocalcin (BGP), urinary excretion of hydroxyproline (HOP/Cr), and pyridinoline crosslinks (Pyr/Cr). Bone mineral density
was measured at the distal radius (BMD-R) and at the lumbar spine (BMD-LS) by dual X-ray absorptiometry (DXA). Ultrasound
parameters were also performed at the calcaneous with the Achilles device and at the phalanxes with DBM Sonic 1200. A significant
reduction (P < 0.001) in BMD and in ultrasound parameters was found in OI patients compared with normals. PICP was significantly reduced
in the OI patients compared with controls (P < 0.001); other markers of bone turnover were higher in OI than in controls, but the difference did not reach the statistical
significance. A significant correlation (P < 0.05) was found between PICP and BMD at the lumbar spine and between PICP and ultrasound parameters at the calcaneous.
On the basis of our data, we conclude that patients with OI show low values of BMD and ultrasound parameters; therefore in
these patients, not only is bone mass disturbed but also bone quality. The reduced levels of PICP in OI patients confirm that
most OI patients have defects in collagen I biosynthesis. These defects may contribute to the fragility of OI bone by interfering
with complete mineralization and/or normal tissue structure. PICP may be considered a useful marker in the clinical management
of OI.
Received: 26 March 1998 / Accepted: 15 January 1999 相似文献
12.
The effects of 10% dietary xylitol supplementation in ovariectomized rats were studied on the degradation of bone organic
and inorganic structures. The osseal concentrations of hydroxyproline, pyridinoline, and deoxypyridinoline were analyzed by
high-performance liquid chromatography. Bone resorption was measured in [3H]tetracycline-prelabeled rats by urinary excretion of 3H, and by the amount of 3H preserved in bone. Bone trabeculation was measured by a computer image analyzer from sections stained by the method of von
Kossa. The amount of collagen in bone organic fraction was lower in ovariectomized rats as compared with the sham-operated
controls. This most likely is partly a consequence of an increased resorption, and partly a consequence of a higher proportion
of immature periosteal bone in the ovariectomized animals, leading to a higher ratio of noncollagenous protein to collagen.
The number of pyridinium crosslinks was lower in proportion, indicating no selective changes in the structure of collagen.
Dietary xylitol significantly retarded the ovariectomy-associated decrease in the relative amount of collagen and the number
of its mature crosslinks. Ovariectomy doubled the excretion of 3H and caused a significant decrease in the amount of 3H preserved in bone; both these changes were significantly retarded by the 10% dietary xylitol supplementation. Ovariectomy
significantly decreased the volume of bone trabeculae, but this effect was also significantly inhibited by the xylitol supplementation
in the diet. In conclusion, these findings suggest a dietary xylitol-induced normalizing effect on the rate of bone turnover
in ovariectomized rats.
Received: 12 February 1996 / Accepted: 20 August 1996 相似文献
13.
D. Borderie B. Cherruau M. Dougados O. G. Ekindjian C. Roux 《Calcified tissue international》1998,62(1):21-25
To evaluate bone biochemical markers as predictors of the efficacy of a hormone replacement therapy (HRT), we studied the
bone changes induced by the cessation and return of ovarian function in 28 patients treated for 6 months with a GnRH agonist.
This model reproduced the effects observed in postmenopausal women with high bone turnover treated with HRT. At the end of
the treatment, Z scores were 1.8 ± 0.3 for Crosslaps (CTx) and deoxypyridinoline (D-Pyr), and 1.1 ± 0.2 for bone alkaline
phosphatase (B-ALP) and osteocalcin (OC). This indicated an imbalance in bone remodeling with a high bone resorption. Bone
mineral density (BMD) fell by 4.2 ± 2.5%. The changes in BMD between the 6th and 12th months were 0.34 ± 2.24 and −1.73 ±
3.25% at the lumbar spine and the femoral neck, respectively. Biochemical markers except urinary calcium and hydroxyproline
measured at 6 months were positively correlated with the BMD changes at the lumbar spine. After the resumption of menstruation,
13 of 28 women displayed positive spine BMD changes between the 6th and 12th months; in this group, bone biochemical markers
measured at 6 months were significantly higher (P= 0.02). Stepwise regression analysis showed that the association of B-ALP and D-Pyr measured at 6 months explained 40% of
BMD variance and the association of B-ALP, PTH, and estradiol 56%. We conclude that measuring individual biochemical bone
markers can help to predict the bone effect of an increase in the circulating estradiol in women with ovarian deficiency.
Received: 16 January 1997 / Accepted: 17 June 1997 相似文献
14.
In this population-based study, the relationship between childhood weight and height, and adolescent bone mass and muscle
strength have been studied in 39 girls and 48 boys. Total body and femoral neck bone mass measurements (bone mineral content,
BMC and bone mineral density, BMD) were made by dual X-ray absorptiometry. Quadriceps muscle strength was measured. Mean age
at the time of measurement was 15.1 years for girls and boys. Results were individually linked to data on childhood (birth
to 6 years of age) weight and height, taken from community health records. Childhood weight was found to be predictive of
adolescent total body BMC (TBMC). However, this was not the case when correlating childhood weight and total body BMD (TBMD),
suggesting that growth determines the size of the skeleton, whereas the density within that bone envelope is to a greater
extent governed by other factors. Further, in a multiple regression model we found that the combined effect of childhood weight
and height was significantly correlated with adolescent quadriceps muscle strength.
Received: 25 July 1995 / Accepted: 10 December 1996 相似文献
15.
H. N. Rosen A. C. Moses J. Garber D. S. Ross S. L. Lee S. L. Greenspan 《Calcified tissue international》1998,63(5):363-368
Biochemical markers of bone turnover are often measured in patients treated with antiresorptive agents to monitor the effects
of therapy. In order for a change in these markers to clearly indicate treatment effect, the change in the markers must exceed
the amount of spontaneous variation typically seen with no treatment. Based on the measured long-term variability of markers
in untreated patients, we defined a minimum significant change (MSC), that is, a change that was sufficiently large that it
was unlikely to be due to spontaneous variability. We also examined the changes in markers of bone turnover in subjects treated
with pamidronate to see how often observed changes in turnover after treatment exceeded the MSC. We found that urinary markers
of bone resorption are best measured on 2-hour fasting samples, because results on random urine showed poor precision and
less decline with therapy. We also found that of all the markers, urinary N-telopeptide cross-links (NTX) had the greatest
decline after therapy (58%), although it also had the highest long-term variability (29.5%). The marker that most often showed
a decline with treatment that exceeded the MSC was serum bone-specific alkaline phosphatase where 74% of observed changes
exceeded the MSC. Other markers that often showed a decline with treatment that exceeded the MSC were 2-hour fasting urine
NTX and free deoxypyridinoline, where 57% and 48%, respectively, of changes in therapy exceeded the MSC. The ideal marker
would combine the large decline after treatment characteristic of NTX (60–70%) with the good precision of bone-specific alkaline
phosphatase.
Received: 3 November 1997 / Accepted: 20 February 1998 相似文献
16.
Etherington J Keeling J Bramley R Swaminathan R McCurdie I Spector TD 《Calcified tissue international》1999,64(5):389-393
To measure the physiological changes in bone in response to strenuous exercise we performed a prospective study of male army
recruits over 10 weeks of basic training. Measurements performed at the start and completion of training consisted of ultrasound
(US) measurements of the heel: velocity of sound (VOS in m/seconds) and broadband ultrasound attenuation (BUA in dB/MHz) and
bone turnover markers; osteocalcin (OC), bone-specific alkaline phosphatase (BALP), and tartrate-resistant acid phosphatase
(TRAP). Forty subjects were recruited for the study and 26 completed training. Over the 10-week study period there was a significant
1.7% fall in mean VOS [mean paired difference (mpd) 27.2 m/second, SEM 9.5 (95% CI 7.5–46.8) P= 0.009] and a nonsignificant 3.4% increase in BUA (P= 0.159). There were significant falls in markers of bone formation OC [11.6%, mpd 0.11 μg/liter (95% CI 0.07–0.14) P < 0.001] and BALP [13.3%, mpd 3.49 U/liter (CI 0.80–6.18) P= 0.013] and a nonsignificant 9.5% fall in TRAP a marker of bone resorption. The 10 recruits subsequently injured had a significantly
lower VOS on entry [mean difference 24.2 m/seconds (95% CI 4.6–43.7) P= 0.017] and nonsignificantly raised BUA and baseline levels of all bone markers. The ultrasound changes may be accounted
for by increase in trabecular separation and a fall in trabecular connectivity due to microfracture. The decrease in bone
markers implies a fall in bone turnover.
Received: 26 June 1997 / Accepted: 26 August 1998 相似文献
17.
Fluoride Treatment Increased Serum IGF-1, Bone Turnover,and Bone Mass,but Not Bone Strength,in Rabbits 总被引:3,自引:0,他引:3
C. H. Turner L. P. Garetto A. J. Dunipace W. Zhang M. E. Wilson M. D. Grynpas D. Chachra R. McClintock M. Peacock G. K. Stookey 《Calcified tissue international》1997,61(1):77-83
We hypothesized that fluoride partly acts by changing the levels of circulating calcium-regulating hormones and skeletal
growth factors. The effects of oral fluoride on 24 female, Dutch-Belted, young adult rabbits were studied. The rabbits were
divided into two study groups, one control and the other receiving about 16 mg fluoride/rabbit/day in their drinking water.
After 6 months of fluoride dosing, all rabbits were euthanized and bone and blood samples were taken for analyses. Fluoride
treatment increased serum and bone fluoride levels by over an order of magnitude (P < 0.001), but did not affect body weight or the following serum biochemical variables: urea, creatinine, phosphorus, total
protein, albumin, bilirubin, SGOT, or total alkaline phosphatase. No skeletal fluorosis or osteomalacia was observed histologically,
nor did fluoride affect serum PTH or Vitamin D metabolites (P > 0.4). BAP was increased 37% (P < 0.05) by fluoride; serum TRAP was increased 42% (P < 0.05); serum IGF-1 was increased 40% (P < 0.05). Fluoride increased the vertebral BV/TV by 35% (P < 0.05) and tibial ash weight by 10% (P < 0.05). However, the increases in bone mass and bone formation were not reflected in improved bone strength. Fluoride decreased
bone strength by about 19% in the L5 vertebra (P < 0.01) and 25% in the femoral neck (P < 0.05). X-ray diffraction showed altered mineral crystal thickness in fluoride-treated bones (P < 0.001), and there was a negative association between crystal width and fracture stress of the femur (P < 0.02). In conclusion, fluoride's effects on bone mass and bone turnover were not mediated by PTH. IGF-1 was increased by
fluoride and was associated with increased bone turnover, but was not correlated with bone formation markers. High-dose fluoride
treatment did not improve, but decreased, bone strength in rabbits, even in the absence of impaired mineralization.
Received: 5 November 1996 / Accepted: 3 January 1997 相似文献
18.
Histomorphometric Evaluation of the Effects of Ovariectomy on Bone Turnover in Rat Caudal Vertebrae 总被引:1,自引:0,他引:1
Miyakoshi N Sato K Abe T Tsuchida T Tamura Y Kudo T 《Calcified tissue international》1999,64(4):318-324
The purpose of this study was to learn whether caudal vertebrae can be used to evaluate the effects of ovariectomy (OVX)
in rats. Seven-month-old female Wistar rats were divided into two groups: the OVX group and the untreated control group. All
rats were killed at 8 weeks and their 4th lumbar (L4), 1st caudal (C1), 3rd caudal (C3), and 5th caudal (C5) vertebrae were
processed undecalcified and sectioned with Villanueva bone stain for quantitative bone histomorphometry. Both length of vertebral
bodies and the cancellous tissue area in C1 were similar in size to L4 but significantly bigger than C3 and C5. Within the
groups, cancellous bone volume (BV/TV) and trabecular thickness in both groups gradually increased in caudal vertebrae in
relation to the distal direction. Between the groups, OVX rats exhibited a significantly lower BV/TV relative to control rats
at L4 and C1, however, no significant difference were seen at C3 and C5. Bone formation-related parameters such as osteoid
and mineralizing surface, and eroded surface were higher in the OVX group than in the control group in caudal as well as in
lumbar vertebrae. By quantitative analysis of bone marrow composition, yellow marrow volume in C3 and C5 was significantly
higher than that in L4 and C1, in both groups. Our results suggest that C1 is similar to L4 in size, bone turnover, and bone
marrow composition. However, further experiments are needed to evaluate the possibility that C1 vertebra could be used as
an alternative site for histomorphometric evaluation of bone changes in OVX rats.
Received: 12 August 1997 / Accepted: 11 May 1998 相似文献
19.
P. Peichl A. Griesmacher P. Pointinger R. Marteau W. Hartl W. Gruber H. Bröll 《Calcified tissue international》1998,62(5):388-394
In an epidemiological study, markers of bone formation (serum osteocalcin and C-terminal propeptide of type I collagen) and
bone resorption [urinary type I collagen peptides (Crosslaps), urinary total pyridinoline (TPYRI), urinary deoxypyridinoline
(DPYRI) as well as female sex hormones (serum estradiol)], follicle-stimulating hormone (FSH) and luteinizing hormone were
measured in 237 women. This cohort aged 44–66 years, came for their first medical examination since menopause to the outpatient
menopause clinic at the Kaiser-Franz-Josef-Hospital, Vienna. The women were all 0.5–5.0 years since cessation of menses and
were not taking medications other than hormone replacement therapy [52 cases, 21.9%)] and had no diseases known to affect
bone and mineral metabolism. The best correlation was found between urinary DPYRI and urinary TPYRI (r = 0.63, P= 0.0001), followed by urinary Crosslaps and urinary DPYRI (r = 0.47, p = 0.0001). Only weak but significant correlations
between E2 and urinary Crosslaps (r =−0.21, P < 0.0001) as well as serum E2 and serum osteocalcin (r =−0.16, P= 0.0007), were observed. Of the 237 women 53% suffered from a severe E2 deficiency (E2 < 10.0 ng/liter). In these patients, urinary Crosslaps (+48%) and serum osteocalcin (+22%) were significantly higher (P < 0.0001) compared with those patients with E2 levels > 10 ng/liter. Women with E2 levels >10 ng/liter were further subdivided into those with and without sex hormone replacement therapy, whereby no statistical
differences in any of the biochemical markers could be observed between these groups. We could clearly demonstrate that in
postmenopausal women suffering from severe E2 deficiency (E2 < 10 ng/liter), urinary Crosslaps and serum osteocalcin are significantly increased, indicating in principle a clear correlation
between E2 deficiency and these markers of bone turnover.
Received: 3 February 1997 / Accepted: 15 October 1997 相似文献
20.
I. Gorai Y. Taguchi O. Chaki M. Nakayama H. Minaguchi 《Calcified tissue international》1997,60(4):317-322
Urinary excretion of cross-linked N-telopeptide of type I collagen (NTx) has been reported to be a specific marker of bone
resorption [18]. We assessed a new immunoassay for NTx as an indicator of changes in bone resorption caused by spontaneous
menopause and compared cross-sectionally the levels of urinary NTx, hydroxylysylpyridinoline (HP), lysylpyridinoline (LP),
hydroxyproline (OH-Pr), other serum biochemical indices, and lumbar spine and proximal femur bone mineral density (BMD). Eighty-one
Japanese women aged 22–77 participated in this study; 36 were premenopausal and 45 were postmenopausal. Urinary HP, LP, and
NTx stayed at low levels in the premenopausal period and rose 21%, 30%, and 67% in the postmenopausal period, respectively.
The rise in LP and NTx was statistically significant (P < 0.01), suggesting that NTx is mostly released from bone matrix when bone resorption is accelerated. When premenopausal
women were divided into two age groups and postmenopausal women were divided into two groups according to years since menopause
(YSM) there were significant differences in LP and NTx between women <4 YSM and women aged <40 and those women aged 41+ (P < 0.01 and P < 0.05, respectively). A significant 110% increase in urinary NTx and a 48% increase in urinary LP were observed in postmenopausal
women compared with age-matched premenopausal women aged 45–55. All biochemical markers other than serum PTH correlated significantly
with each other (r = 0.243–0.858, P < 0.05–0.0001). Urinary NTx inversely correlated with lumbar spine BMD. When postmenopausal women were divided into three
groups, the correlation between bone resorption and formation markers in women 0-1 YSM was greater than in women 2–10 YSM
and in women 11 + YSM, indicating that resorption and formation are coupled at the early postmenopausal period. We conclude
that urinary NTx is responsive to changes in bone metabolism caused by estrogen deficiency and may be a more sensitive and
specific marker than HP, LP, or OH-Pr in the early postmenopausal years.
Received: 15 February 1995 / Accepted: 18 October 1996 相似文献