碘伏消毒液毒理学试验研究

目的研究碘伏（AEO-1）消毒液使用的安全性。方法采用小鼠急性经口毒性、小鼠骨髓细胞微核、一次破损皮肤刺激、多次完整皮肤刺激、急性眼刺激、一次阴道黏膜刺激及亚急性毒性等试验。结果该碘伏消毒液LD50大于5000mg／kg,属实际无毒,小鼠骨髓细胞微核试验为阴性,碘伏消毒液原液对家免皮肤、眼及阴道黏膜刺激的刺激指数均为0,均属无刺激性;亚急性毒性试验,当高剂量组为1．0g／kg时,各项检测指标均未观察到异常变化。结论提示AEO-1为载体的碘伏消毒液在使用浓度下是安全的。     

疱疹宁乳膏皮肤毒性实验研究

目的：考察疱疹宁乳膏对动物皮肤的毒性作用。方法：采用健康大白鼠进行皮肤急性毒性试验；用健康家兔进行皮肤刺激性试验；用健康豚鼠进行皮肤过敏试验。结果：疱疹宁乳膏对大鼠完整皮肤及破损皮肤急性吸收毒性，对家兔完整皮肤和破损皮肤无刺激性，对豚鼠完整皮肤无致敏作用。结论：疱疹宁乳膏用于治疗单纯疱疹和带状疱疹是较安全的。     

黄术消肿洗剂多次给药对家兔皮肤刺激性实验

目的探讨黄术消肿洗剂对家兔皮肤的刺激性,为临床安全用药提供安全性依据。方法对健康大耳白家兔进行急性皮肤刺激、破损皮肤刺激及多次皮肤刺激实验,观察黄术消肿洗剂对家兔完整和破损皮肤的刺激性。结果黄术消肿洗剂对家兔完整皮肤和破损皮肤未见刺激性。结论黄术消肿洗剂对家兔皮肤无明显刺激作用,临床应用安全可靠。     

痤疮灵皮肤毒性实验研究

目的:考察痤疮灵对动物皮肤的毒性作用。方法:采用健康大白鼠进行皮肤急性毒性试验;用健康家兔进行皮肤刺激性试验;用健康豚鼠进行皮肤过敏试验。结果:痤疮灵对大鼠完整皮肤及破损皮肤无皮肤急性吸收毒性,对家兔完整皮肤和破损皮肤无刺激性,对豚鼠完整皮肤无致敏作用。结论:座疮灵用于治疗座疮是较安全的。     

祛痘新肤乳的皮肤毒性实验

目的 考察祛痘新肤乳的安全性. 方法 对健康SD大鼠皮肤进行急性毒性实验; 对健康新西兰兔进行皮肤刺激实验; 用健康豚鼠进行皮肤变态反应实验. 结果祛痘新肤乳对健康大鼠完整皮肤和破损皮肤均未引起急性毒性反应, 对家兔完整皮肤无刺激性, 对破损皮肤有轻微刺激性; 且对豚鼠皮肤无致敏作用. 结论祛痘新肤乳外用安全、无毒.     

糖足洗液皮肤安全性的动物实验

目的：观察糖足洗液对动物皮肤的毒性作用。方法皮肤急性毒性试验采用最大给药量法,观察大鼠完整皮肤及破损皮肤接触糖足洗液后所产生的急性毒性反应和死亡情况;皮肤刺激性试验观察实验兔完整皮肤皮肤和破损皮肤多次接触糖足洗液后所产生的刺激反应情况和恢复情况;皮肤过敏性试验观察豚鼠经皮肤重复接触糖足洗液后所产生的皮肤过敏反应及过敏强度。结果糖足洗液对大鼠完整皮肤及破损皮肤未见急性毒性反应、对实验兔完整皮肤皮肤和破损皮肤无刺激性、对豚鼠皮肤无致敏作用。结论糖足洗液皮肤急性毒性、皮肤刺激以及皮肤过敏试验结果未见明显毒性反应,用于糖尿病足的治疗是较安全的。     

疱疹宁乳膏皮肤毒性实验研究

目的:考察疱疹宁乳膏对动物皮肤的毒性作用。方法:采用健康大白鼠进行皮肤急性毒性试验;用健康家兔进行皮肤刺激性试验;用健康豚鼠进行皮肤过敏试验。结果:疱疹宁乳膏对大鼠完整皮肤及破损皮肤无皮肤急性吸收毒性,对家兔完整皮肤和破损皮肤无刺激性,对豚鼠完整皮肤无致敏作用。结论:疱疹宁乳膏用于治疗单纯疱疹和带状疱疹是较安全的。     

氯己定碘溶液剂的初步毒理学评价

摘要：目的:检测氯己定碘溶液剂的安全性。方法:通过急性经口毒性实验、皮肤刺激实验、急性眼刺激性实验、小鼠骨髓嗜多染红细胞微核实验4个实验对氯己定碘溶液剂进行初步毒理学评价。结果:氯己定碘水溶液制剂对皮肤、眼睛均无刺激性对骨髓嗜多染红微核细胞及红细胞也无抑制作用。结论:氯己定碘水溶液制剂属于低毒性消毒剂。     

复方冰甲乳膏皮肤刺激性研究

目的:研究复方冰甲乳膏的刺激性。方法:采用家兔皮肤刺激性实验,分完整皮肤组和破损皮肤组,同一兔的左右背两侧分别涂抹复方冰甲乳膏和赋形剂,每次1 g,单次和连续7 d给药后,对家兔皮肤刺激性、刺激强度评分并观察恢复情况。结果:完整皮肤组家兔背两侧皮肤无红斑、水肿等现象,破损皮肤组家兔皮肤涂复方冰甲乳膏侧有轻度刺激性。结论:复方冰甲乳膏对家兔完整皮肤无刺激性,对家兔破损皮肤有轻度刺激性。     

羊毛防虫蛀剂的急性毒性及刺激实验

为了研究羊毛防虫蛀剂对人的毒性及安全性,作者用小鼠进行了毒性实验。用80％乙醇溶液,在豚鼠完整皮肤及破损皮肤上进行了皮肤刺激性实验。用10％生理盐水混悬液对家兔眼结膜进行了刺激性实验。结果表明：小鼠口服羊毛防虫蛀剂的半敷致死量为9.2g/kg。对豚鼠完整皮肤及破损皮肤均无明显的刺激性,对家兔眼结膜可产生轻度刺激。研究证明：该羊毛防虫蛀剂对人体是安全的。     

伤必止的皮肤毒性实验研究

目的研究伤必止对动物皮肤的毒性作用。方法对健康家兔进行皮肤急性毒性实验、皮肤刺激性实验,对健康豚鼠进行皮肤过敏实验。结果伤必止对家兔完整及破损皮肤均未引起急性毒性反应和刺激反应,对豚鼠无致敏作用。结论伤必止应用于临床安全、无毒。     

Maleic acid dimethylester: evaluation of dermal toxicity and genotoxicity

Maleic acid dimethylester (MAD) was investigated in acute and subacute dermal toxicity studies, for sensitization potential, and for in vivo and in vitro genotoxicity. The acute dermal toxicity in rats was low (LD50 greater than 2000 mg/kg body weight). Only local effects, erythema and necrosis, occurred at the site of application. Corresponding dose-related effects were observed in a 28-day repeated dermal toxicity study in rats. Treatment-related systemic alterations were observed in feed consumption, body weights, haematology and clinical chemistry at 170 and 500 mg MAD/kg body weight. Based on the results of this study, the no-toxic-effect level of MAD was considered to be 60 mg/kg body weight/day. However, slight dermal irritative effects were also present at the lowest dose level (60 mg/kg body weight). The primary skin irritation test in rabbits showed only slight erythema and oedema. The results of the maximization test in guinea-pigs indicated a clear sensitizing potential of MAD. In the Ames test, with five strains of Salmonella typhimurium, MAD was not mutagenic up to the highest dose level of 5000 micrograms/plate. In the micronucleus test, in which mice were given 1000 mg MAD/kg body weight by gavage the compound revealed no clastogenic effects.     

Safety and toxicological evaluation of Aflapin®: A novel Boswellia-derived anti-inflammatory product

Boswellia serrata gum resin has been used for treatment of various ailments in different cultures for thousands of years. Aflapin^® is a novel synergistic composition derived from B. serrata gum resin (Indian Patent Application No. 2229/CHE/2008). Aflapin is significantly better as an anti-inflammatory agent compared to the Boswellia extracts presently available in the market. To assess the safety of Aflapin, a battery of acute and sub-acute toxicity studies were conducted in various animal models according to the OECD test guidelines. The acute oral LD50 of Aflapin was greater than 5000?mg/kg in female Sprague Dawley (SD) rats. Acute dermal LD50 of Aflapin was greater than 2000?mg/kg in SD rats. A primary dermal irritation study conducted using New Zealand White rabbits indicated that Aflapin is non-irritating to skin. Aflapin caused minimal ocular irritation in a primary eye irritation test conducted on New Zealand Albino rabbits. A repeat dose 28-day sub-acute oral toxicity study in SD rats demonstrated no significant signs of toxicity. Various evaluations including hematology, clinical chemistry, gross necropsy, and histopathology did not show any significant adverse changes. The NOAEL of Aflapin was found to be greater than 2500?mg/kg body weight. These studies demonstrate broad spectrum safety of Aflapin in animal models.     

拟原白头翁素A毒性试验研究

目的为了对拟原白头翁素Ａ进行初步毒理学安全性评价。方法采用大鼠急性经口和经皮毒性试验，大鼠皮肤和大白兔眼粘膜刺激试验，Ａｍｅｓ试验，小鼠骨髓微核试验，小鼠睾丸初级精母细胞染色体畸变试验。结果大鼠急性经口ＬＤ５０４．６７３９（ＩＣ４．０６１４～５．３７８７）ｇ·ｋｇ－１，经皮ＬＤ５０＞２．０ｇ·ｋｇ－１，根据化合物毒性分级为低毒；对大鼠皮肤无刺激作用，对大白兔眼粘膜有中等刺激；Ａｍｅｓ试验、小鼠骨髓微核试验和小鼠睾丸初级精母细胞染色体畸变试验阴性。结论拟原白头翁素Ａ为低毒、致突变试验阴性的化合物。     

Safety and toxicological evaluation of undenatured type II collagen

Previous research has shown that undenatured type II collagen is effective in the treatment of arthritis. The present study evaluated the broad-spectrum safety of UC-II by a variety of toxicological assays including acute oral, acute dermal, primary dermal irritation, and primary eye irritation toxicity. In addition, genotoxicity studies such as Ames bacterial reverse mutation assay and mouse lymphoma tests, as well as a dose-dependent 90-day sub-chronic toxicity study were conducted. Safety studies indicated that acute oral LD₅₀ of UC-II was greater than 5000?mg/kg in female Sprague-Dawley rats. No changes in body weight or adverse effects were observed following necropsy. Acute dermal LD₅₀ of UC-II was determined to be greater than 2000?mg/kg. Primary skin irritation tests conducted on New Zealand Albino rabbits classified UC-II as slightly irritating. Primary eye irritation tests conducted on rabbits indicated that UC-II was moderately irritating to the eye. UC-II did not induce mutagenicity in the bacterial reverse mutation test in five Salmonella typhimurium strains either with or without metabolic activation. Similarly, UC-II did not induce a mutagenic effect in the gene mutation test in mouse lymphoma cells either with or without metabolic activation. A dose-dependent 90-day sub-chronic toxicity study revealed no pathologically significant changes in selected organ weights individually or as percentages of body or brain weights. No significant changes were observed in hematology and clinical chemistry. Therefore, the results from the current study show a broad-spectrum safety profile of UC-II.     

盐酸普萘洛尔乳膏的安全性考察

目的考察盐酸普萘洛尔乳膏对动物皮肤的毒性作用。方法采用不同浓度(2%、5%、10%)的盐酸普萘洛尔乳膏对家兔进行皮肤刺激性试验、对豚鼠进行过敏性试验、对Wistar大鼠进行急性毒性试验及长期毒性试验。结果 2%、5%盐酸普萘洛尔乳膏对完整、破损皮肤均无刺激性、急性毒性,对完整皮肤无明显毒性,不产生致敏作用,10%有轻微刺激性,可自行缓解。结论 2%~5%盐酸普萘洛尔乳膏是较安全的外用制剂。     

Acute toxicity studies with insect attractants.

Eight insect attractants and one inhibitor of a sex attractant were tested for acute oral and aerosol inhalation toxicity in rats, for acute dermal toxicity, eye irritation, and primary skin irritation on rabbits, and for toxicity to rainbow trout and bluegill sunfish. Except for phenethyl propionate + eugenol, 7:3 (acute dermal LD50, 1220 mg/kg), none of the test materials was classified as more than slightly toxic. (Z)-7-Dodecen-1-ol acted as a primary skin irritant, since it caused superficial chemical burns.     

Acute dermal toxicity of two quarternary organophosphonium salts in the rabbit

Tetrabutyl phosphonium chloride and tetrabutyl phosphonium bromide were evaluated for their potential to cause primary dermal irritation and acute dermal toxicity in rabbits. Both chemicals were found to not only be severely irritating to skin, but the pure hygroscopic chemicals caused death in more than half the number of rabbits used in the primary dermal irritation tests. Further investigations revealed the single-application dermal LD50 of tetrabutyl phosphonium chloride (Bu4PCl) (using an ethylene carbonate (EC) vehicle) was 600 mg/kg for male rabbits and 500 mg/kg for female rabbits. The single-application dermal LD50 of tetrabutyl phosphonium bromide (Bu4PBr) (using the EC vehicle) was 700 mg/kg for male rabbits and 850 mg/kg for female rabbits. The dermal LD50 of pure undiluted Bu4PCl was 225 mg/kg for male rabbits. These data indicate that Bu4PCl and Bu4PBr represent a substantial acute dermal toxicity hazard.     

Genotoxicity,acute oral and dermal toxicity,eye and dermal irritation and corrosion and skin sensitisation evaluation of silver nanoparticles

Abstract

To clarify the health risks related to silver nanoparticles (Ag-NPs), we evaluated the genotoxicity, acute oral and dermal toxicity, eye irritation, dermal irritation and corrosion and skin sensitisation of commercially manufactured Ag-NPs according to the OECD test guidelines and GLP. The Ag-NPs were not found to induce genotoxicity in a bacterial reverse mutation test and chromosomal aberration test, although some cytotoxicity was observed. In acute oral and dermal toxicity tests using rats, none of the rats showed any abnormal signs or mortality at a dose level of ～ 2000 mg/kg. Similarly, acute eye and dermal irritation and corrosion tests using rabbits revealed no significant clinical signs or mortality and no acute irritation or corrosion reaction for the eyes and skin. In a skin sensitisation test using guinea pigs, one animal (1/20) showed discrete or patchy erythema, thus Ag-NPs can be classified as a weak skin sensitiser.     

甲磺酸帕珠沙星搽剂皮肤用药安全性实验

目的：对甲磺酸帕珠沙星搽剂进行皮肤急性毒性试验、皮肤刺激性试验及皮肤过敏性试验,以考察其皮肤用药安全性。方法：将甲磺酸帕珠沙星搽剂涂抹于家兔背部完整或破损区脱毛皮肤,观察给药后动物局部皮肤红斑及水肿等急性毒性和刺激性情况。采用致敏与激发接触甲磺酸帕珠沙星搽剂,观察豚鼠过敏反应。结果：甲磺酸帕珠沙星搽剂对家兔完整或破损皮肤无明显局部毒性或局部刺激性。对豚鼠皮肤无过敏反应。结论：甲磺酸帕珠沙星搽剂短期皮肤用药是安全的。