大气飘尘对兔肺巨噬细胞体外抗Hela细胞活性的影响

经活化的肺泡巨噬细胞（ＰＡＭ）能抑制Ｈｅｌａ细胞的增殖，其细胞毒性指数为５７．９０±２．４８（Ｐ〈０．０１）。ＰＡＭ的这种细胞毒功能可被大气飘尘（ＡＰ）降低，在ＡＰ浓度为１０～１００μｇ／ｍｌ范围内这种降低作用显剂量－反应关系，在１０，５０，１００μｇ／ｍｌＡＰ作用下，ＰＡＭ对Ｈｅｌａ细胞的细胞毒指数分别为５１．９１±３．６７（Ｐ〉０．０５），４０．８３±２．１４（Ｐ〈０．０１），３２．３３±４     

巨噬细胞功能测定在甲型肝炎患者中的意义

采用荧光标记的葡萄球菌法对４３例甲型肝炎（甲肝）进行了外周血的巨噬细胞功能测定。同时用鲎试剂基质偶氮显色法检测血清中的内毒素。结果发现：４３例甲肝的巨噬细；胞吞噬率（ＰＡＭ）为８２．５８±８．７９％，与正常人（ｎ＝２５）的ＰＡＭ（７１．０８±３．１０％）相比有非常显著的差异（Ｐ＜０．０１），而巨噬细胞吞噬指数（ＰＩＭ）两组比较无统计学差异（５５．４８±３．２７Ｖｓ５６．０１±４．０１，Ｐ＞０．０５）。ＰＡＭ和ＰＩＭ与ＡＬＴ、前白蛋白及总胆红素之间无相关。但甲肝的内毒素浓度（６２．６６±２．０５μｇ／Ｌ）与ＰＡＭ有明显相关（ｒ＝０．７８０４Ｐ＜０．０１），与ＰＩＭ则无关（ｒ＝０．１４６，Ｐ＞０．０５）。我们也观察了甲肝巨噬细胞功能的动态变化，发现随着甲肝的恢复，血清内毒素浓度随之降之正常，ＰＡＭ也趋于正常，而ＰＩＭ则无明显变化。     

外科性黄疸和黄疸型病毒性肝炎患者血清SIgA的变化

外科性黄疸（ｓｕｒｇｉｃａｌｊａｕｎｄｉｃｅ，ＳＪ）患者（３８例）术前血清分泌型ＩｇＡ（ｓｃｒｅｔｏｒｙＩｇＡ，ＳＩｇＡ）含量为１４０．８±５１．３μｇ／ｍｌ，比正常人（３０人）的平均值（８．９±２．１μｇ／ｍｌ）高１５．８倍（Ｐ＜０．０１）．手术解除胆道硬阻后１５～２０ｄ，ＳＪ患者的血清ＳＩｇＡ水平显著下降（Ｐ＜０．０１）；同时，术后胆汁中的ＳｉｇＡ却远高于术中时胆汁ＳＩｇＡ的水平（Ｐ＜０．０１）。黄疸型病毒性肝炎（ｉｃｔｅｒｉｃｖｉｒａｌｈｅｐａｔｉｔｉｓ，ＩＶＨ）患者（３０例）的血清ＳＩｇＡ含量也显著增多（７６．４±２７．８μｇ／ｍｌ，Ｐ＜０．０１），然而却远低于ＳＪ患者的水平（Ｐ＜０．０５）。     

不饱和脂肪酸对肺癌细胞的抑制作用

不饱和脂肪酸（ＬＡ）浓度为２５μｇ／ｍｌ，对人肺腺癌细胞系（ＳＰＣ－Ａ１）细胞和二倍体人胚肺细胞系（ＨＬＦ）细胞抑制率分别为：９８．４±２．２％，１８．０±２．１％（Ｐ＜０．００１）。表现为对恶性细胞的选择性抑制作用。ＳＰＣ－Ａ１对５μｇ／ｍｌ ＬＡ无反应，１μＭ丝裂霉素Ｃ（ＭＭＣ）对ＳＰＣ－Ａ１细胞抑制率为４３．４±２．９％而当用１μｍ ＭＭＣ处理这种对５μｇ／ｍｌ ＬＡ无反应的ＳＰＣ－Ａ１时，     

肝硬变患者血清层粘连蛋白水平的变化

探讨肝硬变患者血清层粘连蛋白水平变化的影响因素及其临床意义。应用放射免疫分析法测定６２例ＬＣ患者血清ＬＭ含量。肝硬变患者血清ＬＭ「１４８．７２±３６．５７）μｇ／Ｌ」，显著高于耐血员对照组「（１０５．８１±１４．５６）μｇ／Ｌ，，Ｐ〈０．０１」。Ｃｈｉｌｄ‘２Ａ，Ｂ，Ｃ各级「（１２５．３５±３０．６２）μｇ／Ｌ，Ｐ〉０．０５，（１４．５６８±２７．９５）μｇ／Ｌ，Ｐ〈０．０１，（１７１．６５±４３     

高渗盐水复苏对失血性休克家兔胃粘膜及肝细胞能量代谢的影响

目的：探讨高渗盐水复苏对失血性休克家兔胃粘膜及肝细胞能量代谢影响的机制．方法：家兔按２０ｍｌ／ｋｇ体重放血致失血性休克，６０ｍｉｎ后分别回输１０％放血量的抗凝自体全血、生理盐水及１２８２ｍｍｏｌ／Ｌ氯化钠高渗盐水．采用高效液相色谱技术检测胃底粘膜细胞及肝细胞ＡＴＰ，ＡＤＰ，ＡＭＰ含量．结果：高渗盐水复苏后，失血性休克家兔胃底粘膜细胞及肝细胞内ＡＴＰ含量［分别为（２１７．６３±７０．２９）ｎｍｏｌ／ｇ和（１７８．７２±５３．６５）ｎｍｏｌ／ｇ］非常显著地高于缺血对照组［（８７．８８±４３．６１）ｎｍｏｌ／ｇ和（７８．１４±２４．２５）ｎｍｏｌ／ｇ］（Ｐ＜０．０１），能荷（分别为０．２８６±０．０５１和０．２１３±０．００２）显著高于缺血对照组动物（０．１７５±０．０９５和０．１２６±０．０３６）（Ｐ＜０．０５）．结论：高渗盐水复苏，具有改善失血性休克家兔胃底粘膜细胞及肝细胞能量代谢的作用     

雷公藤抑制免疫排斥反应的实验研究

目的：通过雷公藤孵化皮片，在同种小鼠间进行移植，探讨雷公藤对免疫排斥反应的抑制作用。方法：采用１５％雷公藤煎剂和４０μｇ／ｍｌ、１００μｇ／ｍｌ、５００μｇ／ｍｌ雷公藤总甙对供鼠皮片浸泡孵化，在昆明种小鼠和ＤＢＡ褐色小鼠间进行皮片移植，观察雷公藤对同种皮片移植免疫排斥的抑制作用。结果：雷公藤煎剂孵化皮片能显著延长移植物的存活时间，两种小鼠皮片移植后存活时间分别为（２２３１±３６０）天、（２１５０±３４０）天，与对照组（１３２０±２２５）天、（１１８１±２７５）天相比有显著意义（Ｐ＜００１）；４０μｇ／ｍｌ雷公藤总甙孵化后皮片存活时间为（１７５４±１４４）天，１００μｇ／ｍｌ为（１９８４±２６３）天，５００μｇ／ｍｌ为（２０９１±３８０）天；雷公藤总甙孵化加腹腔注射，每天４０μｇ／２０ｇ小鼠为（２１３５±２０８）天，１００μｇ为（２２３４±２７５）天，５００μｇ为（１９８２±１１４）天，与对照组（１２８３±０８０）天、（１１９６±１７５）天相比均有显著差异（Ｐ＜００１）；雷公藤总甙单纯孵化各组与孵化加腹腔注射相比，除４０μｇ／ｍｌ组（Ｐ＜００５）外，存活时?     

钩吻碱类提取物对小鼠脾细胞增殖反应的影响

钩吻碱类提取物在浓度大于１．２５μｇ／ｍｌ以上时对混合淋巴细胞培养反应（ＭＬＲ）均有不同程度的抑制作用，具有统计学意义（Ｐ＜０．０５）的最低抑制浓度为２．５μｇ／ｍｌ，抑制率为１８．３％。单独采用Ｃ５７ＢＬ／６ｊ小鼠脾细胞进行实验，促有丝分裂剂为细菌脂多糟（ＬＰＳ５μｇ／ｍｌ），在钩吻碱类提取物浓度为４０μｇ／ｍｌ才出现抑制作用（Ｐ＜０．０５），抑制率为１８％，改用刀豆蛋白Ａ（ＣｏｎＡ２μｇ／ｍｌ）刺激，钩吻碱类提取物最低抑制浓度为２０μｇ／ｍｌ，抑制率为１２．４％（Ｐ＜０．０５）．脾细胞先经ＣｏｎＡ活化．后用白细胞介素２（５ｕ／ｍｌ）维持培养，此时钩吻碱类提取物的最低抑制浓度为４０μｇ／ｍｌ．抑制率为１７．１％（Ｐ＜０．０５）。     

PAOA与四种免疫抑制剂对小鼠体外脾细胞单相和双相混合培养影响初探

本文报道了中药ＰＡＯＡ水提取物对小鼠体外脾细胞单相和双相混合培养有显著的抑制作用。其中１０００μｇ／ｍｌ浓度抑制效应最明显（Ｐ＜０．０１）。并将ＰＡＯＡ与环孢素Ａ（ＣｙｃｌｏｓｐｏｒｉｎｅＡ，ＣｓＡ）、硫唑嘌呤（Ａｚａｔｈｉｏｐｒｉｎｅ，Ａｚａ）、地塞米松（Ｄｅｘａｍｅｔｈａｓｏｎｅ，ＤＸＭ）及环磷酰胺（Ｃｙｃｌｏｐｈｏｓｐｈａｍｉｄｅ，Ｃｙ）四种临床常用的免疫抑制剂作比较，其结果显示ＰＡＯＡ（１０μｇ／ｍｌ，１００μｇ／ｍｌ，１０００μｇ／ｍｌ）的免疫抑制作用明显强于Ａｚａ、ＤＸＭ和Ｃｙ，也不亚于ＣｓＡ的抑制作用。因此，作者认为，ＰＡＯＡ作为免疫抑制剂用于临床治疗将会有广阔前景。     

石楠藤提取物对急性出血坏死性胰腺炎大鼠治疗作用的实验研究

作者报道中药石楠藤提取物对急性出血坏死性胰腺炎（ＡＨＮＰ）大鼠治疗作用的实验研究。将１００只ＳＤ大鼠随机分为三组：①假手术组（ＳＯ组）；②ＡＨＮＰ组（ＡＰ组），该组动物在ＡＨＮＰ诱发后，不做特殊治疗；③石楠藤提取物治疗组（ＳＮ组）。该组动物在ＡＨＮＰ诱发后１０分钟经腹腔内注射石楠藤提取物（１％石楠藤提取物５ｍｌ／ｋｇ体重）。结果表明：ＳＮ组血清淀粉酶值明显下降（ＳＮ组１６１３０±５０３０Ｕ／Ｌ６ｈ、１９２９０±５３８０Ｕ／Ｌ１２ｈｖｓＡＰ组２３７８０±３９６０Ｕ／Ｌ、２８４７０±４１５０Ｕ／Ｌ，Ｐ＜０．０１）。血中ＰＡＦ含量明显降低（ＳＮ组１．３±０．３０ｎｇ／ｍｌ１ｈ、２．０±０．３４ｎｇ／ｍｌ６ｈ、２．１±０．３２ｎｇ／ｍｌ１２ｈｖｓＡＰ组２．２±０．２５ｎｇ／ｍｌ、６．１±０．４２ｎｇ／ｍｌ、１０．１±０．５３ｎｇ／ｍｌ，ｐ＜０．０１）。血浆内毒素含量明显下降（ＳＮ组６９．９±７．２ＥＵ／Ｌ、６ｈ、９１．１±８．４ＥＵ／Ｌ、１２ｈｖｓＡＰ组９８．４±６．９ＥＵ／Ｌ、１５１．７±９．１ＥＵ／Ｌ，Ｐ＜０．０１）。石楠藤提取物治疗组术后平均存活时间明显长于ＡＰ组（ＳＮ组：４３．５ｈ，ＡＰ组１１．５ｈ，ｐ＜０     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.