Acanthamoeba species in Swimming Pools of Cairo,Egypt

Background

The free-living amoebae Acanthamoeba spp. have been recognized as etiologic agents of amoebic encephalitis, keratitis, otitis, lung lesions and other skin infections mainly in immuno-compromised individuals. The purpose of this study is to detect the presence of Acanthamoeba in swimming pools in Egypt using a polymerase chain reaction (PCR) method.

Methods

Water samples were collected from 10 different swimming pools in Cairo, Egypt. Samples were cultured on non-nutrient agar for the detection of Acanthamoeba isolates that were confirmed by PCR amplification using genus specific primers. The molecularly confirmed Acanthamoeba isolates were morphologically identified to the species level.

Results

Members of genus Acanthamoeba were detected in 49.2% of the examined swimming-pool water samples. Morphologically, six Acanthamoeba species were isolated from the examined swimming pool water namely A. polyphaga, A.castellanii, A. rhysodes, A. mauritaniensis, A. royreba and A. triangularis. All the identified species of Acanthamoeba were molecularly confirmed to be related to the genus Acanthamoeba.

Conclusion

The isolated species of Acanthamoeba could provoke variable degrees of infections to the swimmers. The culture method is cheaper and easier than PCR techniques that are faster for the detection of free-living amoebae     

Inpatient and outpatient health care demand in Cairo,Egypt

This paper uses the results of a household survey conducted in Cairo, Egypt in 1992 to examine the factors that influence the demand for inpatient and outpatient health services. Multi-stage discrete choice models of the demand for health care, which identify the importance of individual, household, and facility level variables on each treatment decision, are estimated separately for outpatients and inpatients. Consumers are assumed to decide whether to seek any treatment and then choose between three categories of providers: a large public hospital (Embaba Hospital), all other public providers, and private/charitable providers. The results confirm that more affluent consumers prefer the higher cost, higher quality private and charitable hospitals. Age, sex, education, and insurance are also found to strongly impact the use of medical services. The results are suggestive but do not conclusively show that inpatient care is less price responsive than outpatient care. Price responsiveness of inpatient and outpatient demand are imprecisely estimated because price is highly correlated with quality, and the available data on facility quality do not permit us to adequately control for quality variations across facilities.     

Seasonal variations of polychlorinated biphenyls in ambient air in Zagreb,Croatia

Levels of polychlorinated byphenils (PCBs) were monitored continuously in Zagreb between June 1999 and February 2000 and analysed qualitatively and quantitatively using high resolution gas chromatography (HRGC). Total PCBs were determined with respect to Aroclor 1260, which was used as the standard reference. The concentrations of PCBs ranged from 1.6 to 136 pg/m3 and were higher in warmer seasons (temperatures above 10 degrees C; 5.1-136 pg/m3) than in colder seasons (temperatures from -10 degrees C to about 10 degrees C; PCB range: 1.6-23.5 pg/m3). The increase in PCBs concentration with temperature followed an exponential curve.     

Year-to-year variations in annual average indoor 222Rn concentrations

Annual average indoor 222Rn concentrations in 40 residences in and around Grand Junction, CO, have been measured repeatedly since 1984 using commercial alpha-track monitors (ATM) deployed for successive 12-mo time periods. Data obtained provide a quantitative measure of the year-to-year variations in the annual average Rn concentrations in these structures over this 6-y period. A mean coefficient of variation of 25% was observed for the year-to-year variability of the measurements at 25 sampling stations for which complete data were available. Individual coefficients of variation at the various stations ranged from a low of 7.7% to a high of 51%. The observed mean coefficient of variation includes contributions due to the variability in detector response as well as the true year-to-year variation in the annual average Rn concentrations. Factoring out the contributions from the measured variability in the response of the detectors used, the actual year-to-year variability of the annual average Rn concentrations was approximately 22%.     

Households, resources, and child health in a self-help settlement in Cairo, Egypt

The health of children is produced within the social structures of daily life. This paper looks at children in a large, self-help settlement within the city of Cairo, Egypt. It shows how the social and physical conditions of the community, and the specific resources of households, condition the processes by which better or worse health is produced for children under the age of 3. Health status is measured by anthropometric observation and by household survey. Variables are defined and positioned in the analysis according to a framework that links biological outcomes to social determinants through intermediate factors. The analysis makes use of multivariate modeling, as well as qualitative data collected by direct observation in the community.     

The logic of well being: Therapeutic narratives in Cairo,Egypt

This paper examines understandings of curative action among women in a traditional (baladi) quarter of Cairo, Egypt. It is based on research in a mother-childhealth (MCH) clinic and the surrounding urban area. It probes healing strategies and understandings revealed in therapeutic narratives. These narratives are commentaries on disease progression, curative action and surrounding events—both relevant and irrelevant. Fragments of narratives embedded in conversation are framed by stylistic shifts; they are eventually codified into elaborated accounts which are referenced for years after the illness episode.Therapeutic narratives present a system of knowledge which mediates between the everyday experience of diagnosis and curative action and the cultural context (ethnoetiology, ethnophysiology, concepts of fate and envy). They provide a biographic and experimental context for perception and diagnosis of illness. They also facilitate transformation of diagnosis and prognosis. The article presents baladi therapeutic narratives as a specific instance of a culture-wide phenomenon of ‘tacking down’ illness episodes to make sense of them. Discussion also highlights the baladi-specific curative system with its principles of regularity and fluidity, and the baladi-specific logic of proximity, specificity, and multiplicity which organizes baladi therapeutic narratives.Cases presented demonstrate how baladi women evaluate the meaning of experience and action while recounting illness episodes and how they corroborate their curative action with past experience and community standards. Through continual re-evaluation a finely woven web of rationale evolves as a woman with a difficult problem such as delayed conception or a persistently weak child moves through curing strategies. Diagnosis is always subject to revision; explanations of what is happening are constantly generated—whether in respect of mundane activity or of the event's meaning.Narrative recounting is the locus par excellence for the aggregation of cognitions concerning a whimsical universe where baladi people's main power lies in their skill at verbal manipulation. However, it is not used in lieu of action. Narratives provide an arena for the negotiation of reality and of correct action; they do not discount reality, but make sense of it.     

Seasonal variations in air pollution particle-induced inflammatory mediator release and oxidative stress

Health effects associated with particulate matter (PM) show seasonal variations. We hypothesized that these heterogeneous effects may be attributed partly to the differences in the elemental composition of PM. Normal human bronchial epithelial (NHBE) cells and alveolar macrophages (AMs) were exposed to equal mass of coarse [PM with aerodynamic diameter of 2.5-10 microm (PM(2.5-10)], fine (PM(2.5)), and ultrafine (PM(<0.1)) ambient PM from Chapel Hill, North Carolina, during October 2001 (fall) and January (winter), April (spring), and July (summer) 2002. Production of interleukin (IL)-8, IL-6, and reactive oxygen species (ROS) was measured. Coarse PM was more potent in inducing cytokines, but not ROSs, than was fine or ultrafine PM. In AMs, the October coarse PM was the most potent stimulator for IL-6 release, whereas the July PM consistently stimulated the highest ROS production measured by dichlorofluorescein acetate and dihydrorhodamine 123 (DHR). In NHBE cells, the January and the October PM were consistently the strongest stimulators for IL-8 and ROS, respectively. The July PM increased only ROS measured by DHR. PM had minimal effects on chemiluminescence. Principal-component analysis on elemental constituents of PM of all size fractions identified two factors, Cr/Al/Si/Ti/Fe/Cu and Zn/As/V/Ni/Pb/Se, with only the first factor correlating with IL-6/IL-8 release. Among the elements in the first factor, Fe and Si correlated with IL-6 release, whereas Cr correlated with IL-8 release. These positive correlations were confirmed in additional experiments with PM from all 12 months. These results indicate that elemental constituents of PM may in part account for the seasonal variations in PM-induced adverse health effects related to lung inflammation.     

HIV/AIDS perceptions and risky behaviors in squatter areas in Cairo, Egypt

The aim of this work was to assess HIV/AIDS perceptions and risky behaviors of adults living in squatter areas in order to take decisions about the relevant messages and health education methods that should be used to prevent HIV transmission in the studied communities. Two methods were used to collect data in this cross sectional study, focus group discussion and structured interview. The study was carried out in six squatter areas in Cairo, four of them located in west Cairo and two in east Cairo. Results: 237 community members and community leaders shared in the study, 44.7% were males and 55.3% females, 64.1% were married and 34% single. Community members represented two-thirds of the sample while one-third were community leaders. Participants expressed strong eagerness to know more about HIV/AIDS. Poor knowledge and misconception about HIV/AIDS was remarkable. The study revealed several misconceptions regarding modes of transmission and preventive measures that could be used to protect oneself from HIV/AIDS. Heterosexual extramarital relationships and i.v. drug transmission were the HIV/AIDS risky behaviors considered to be prevalent in these communities. AIDS risky behaviors appear to be more prevalent in the four squatter areas in west Cairo than in the two squatter areas located in east Cairo. Adolescents were considered at high risk of contracting AIDS because of the risky behaviors they practice. Social non-acceptance of females who practice HIV/AIDS risky behaviors is much stronger than non-acceptance of males who practice the same risky behaviors. TV movies were the main source of information about HIV/AIDS. Parents played no role in providing adolescents with information about AIDS. The possible role of teachers in HIV/AIDS education is unclear because of the negative attitude of people towards teachers.     

Hospital waste management at the Ministry of health hospitals in Cairo Governorate,Egypt

Hospitals and clinics have been mistakenly assumed to be healthy places to work. Waste generated from different health facilities (hospitals, centers and clinics) pose some risks on both health and environment. The present study is concerned with analyzing the situation of the administrative structure, clinical activities, and waste management system present in the MOH hospitals in Cairo Governorate. The study was carried out on 24 MOH hospitals of which 14 are general hospitals and 10 are specialized (skin and venereal, ophthalmologic, psychiatric and chest specialities). The study identifies waste generators in each unit and describes and analyzes the waste generated from these hospitals in terms of quality and quantity. The study investigates and assesses the current waste management system in terms of organization, staffing, equipment used among others. The conclusions give a list of the major environmental health problems associated with hospital waste in Cairo.     

Effects of hand hygiene campaigns on incidence of laboratory-confirmed influenza and absenteeism in schoolchildren, Cairo, Egypt

To evaluate the effectiveness of an intensive hand hygiene campaign on reducing absenteeism caused by influenza-like illness (ILI), diarrhea, conjunctivitis, and laboratory-confirmed influenza, we conducted a randomized control trial in 60 elementary schools in Cairo, Egypt. Children in the intervention schools were required to wash hands twice each day, and health messages were provided through entertainment activities. Data were collected on student absenteeism and reasons for illness. School nurses collected nasal swabs from students with ILI, which were tested by using a qualitative diagnostic test for influenza A and B. Compared with results for the control group, in the intervention group, overall absences caused by ILI, diarrhea, conjunctivitis, and laboratory-confirmed influenza were reduced by 40%, 30%, 67%, and 50%, respectively (p<0.0001 for each illness). An intensive hand hygiene campaign was effective in reducing absenteeism caused by these illnesses.