新疆和河南红花中羟基红花黄色素A和山奈素含量分析

目的:高效液相色谱法(HPLC)方法测定新疆和河南红花不同产地、不同品种羟基红花黄色素A和山奈素含量,为评价新疆和河南不同产地和不同品种红花质量提供参考依据。方法:采用高效液相色谱法对新疆44批、河南4批,共48批红花样品进行羟基红花黄色素A测定,对新疆30批样品进行山奈素含量测定。结果:48批不同产地、不同品种红花药材羟基红花黄色素A含量为1.037~2.622,30批新疆样品中山奈素含量为0.288~0.739,昌吉州奇台县云南大头红花羟基红花黄色素A含量平均最高,达2.518%,察布查尔锡伯自治县红花山奈素平均含量最高达0.5876%。结论:不同产地、不同品种红花药材羟基红花黄色素A含量整体趋势为新疆河南,其中新疆三大产区和乌鲁木齐市红花含量趋势为昌吉州地区塔城地区乌鲁木齐市伊犁地区,山奈素含量整体趋势为伊犁地区塔城地区昌吉地区,综合分析新疆昌吉地区红花中羟基红花黄色素A和山奈素含量均较好。     

高效液相色谱法测定虎杖提取物中白藜芦醇的含量

目的：建立虎杖提取物中白藜芦醇的含量测定方法。方法：采用高效液相色谱法测定虎杖提取物中白藜芦醇的含量。色谱条件：流动相：乙腈-水（30∶70）;流速：1.0 ml/min;检测波长：306 nm;柱温：室温;进样量：10 μl。结果：白藜芦醇进样量在0.3～9.6 μg/ml范围内,线性关系良好。结论：本法简便实用、灵敏度高、重现性好,可用于虎杖提取物中白藜芦醇的含量测定。     

苦丁茶中熊果酸含量的测定

目的建立苦丁茶中熊果酸含量测定方法。方法采用高效液相色谱法测定苦丁茶中熊果酸含量,色谱柱:Diam onsil C18(4.6×250m m,5μm);流动相:乙腈-0.05%磷酸(92∶8);流速:1mL.min-1;柱温:30℃;检测波长:220nm。结果熊果酸在0.82～4.10μg范围内线性关系良好,平均回收率为98.2%,RSD=1.3%(n=5),苦丁茶嫩叶中熊果酸的含量为5.93mg/g,老叶中的含量为12.74mg/g。结论该方法简便可行、重复性好,可作为苦丁茶质量评价的依据。     

HPLC法测定新疆白喉乌头不同部位氢溴酸高乌甲素的含量

目的比较新疆尼勒克地区白喉乌头不同部位（根、茎、叶）中氢溴酸高乌甲素的含量。方法采用XBridgeC18生物碱专用色谱柱（250mm×4．6mm,5μm）,以0．04mol／L醋酸铵-乙腈（63：37）为流动相等度洗脱,流速0．8mL／min,柱温40）c,检测波长为252nm,进样量10μL高效液相色谱法测定白喉乌头根、茎、叶中氢溴酸高乌甲素含量。结果白喉乌头中氢溴酸高乌甲素在根中含量为0．2038mg／g,在叶中的含量为0．061lmg／g,在茎中的含量为0．0111mg／g。结论白喉乌头根中氢溴酸高乌甲素含量高于叶和茎中,为白喉乌头资源的进一步利用奠定了一定基础。     

枸骨中芦丁和槲皮素含量测定

目的：采用高效液相色谱法测定枸骨不同部位（嫩叶、老叶、枝、青果、红果）及不同采收期枸骨叶中芦丁和槲皮素的含量。方法采用C18色谱柱（250 mm ×4．6 mm ,5μm）,流动相为甲醇－0．5％磷酸水溶液（47∶53）,流速为0．7 ml／min ,检测波长为356 nm ,柱温25℃,测定枸骨中芦丁和槲皮素含量。结果芦丁和槲皮素线性范围分别为0．52～5．2μg和0．34～3．4μg,相关系数 r均为0．9999,平均回收率分别为98．6％和99．2％,RSD分别为1．70％和1．91％（n＝6）。结论枸骨不同部位及不同采收期枸骨叶中芦丁和槲皮素含量有明显差异。     

银屑胶囊中白藜芦醇含量测定

目的：建立银屑胶裳中白藜芦醇的含量测定方法。方法：采用高效液相色谱法测定银屑胶囊中白藜芦醇的含量。结果：测定结果灵敏可靠,回收率在95．72％～99．92％,RSD为1．55％。结论：本方法可作为制剂质量控制方法。     

离子对反相色谱法测定桑叶中水溶性维生素含量

目的：测定新疆不同地区、不同采集期、不同品种桑叶中水溶性维生素B1、B2(VitB1、VitB2)的含量，以此作为开发利用的标准。方法：采用离子对反相色谱法测定桑叶中的水溶性维生素含量．结果：不同采集期桑叶中VitB1和VitB2的含量基本相同，无明显的变化规律，VitB1和VitB2的平均含量分别为6．10、22．4 μg／g，VitB2的含量大约是VitB1含量的3倍。南疆地区(阿克苏、喀什、和田)的桑叶中VitB1和VitB2的含量比北疆(乌鲁木齐)和东疆(哈密、吐鲁番)地区桑叶中的含量略高。黑桑和白桑中VitB1和VitB2的含量基本相同．VitB1和VitB2的平均回收率分别为97．2％和97．5％，VitB1和VitB2含量的RSD分别为3．45％(n=6)和1．54％(n=6)。结论：用离子对反相色谱法测定桑叶中的水溶性维生素，方法简单、快速，结果准确，重现性好。     

甘肃不同产地三颗针不同部位生物碱的含量测定

目的：研究甘肃产三颗针不同因素对生物碱含量的影响。方法：采用高效液相色谱法测定三颗针生物碱的含量。结果：不同因素下生物碱的含量具有明显差异。结论：三颗针中生物碱的含量与植物的种类、部位等因素有关。     

五种小檗不同部位小檗碱的含量测定

用高效液相色谱法测定了5种小檗的根皮、根木、茎皮，茎木及叶中小檗碱的含量，分布状态以根皮、茎皮较高，木部较低，叶中微量。     

不同产地紫花地丁中秦皮乙素的含量比较

目的：采用高效液相色谱法对21批不同产地紫花地丁药材中秦皮乙素的含量进行了测定。方法：使用Thermo HYpersil-keystong BDS C18高效液相色谱柱,以甲醇-0.1%磷酸（15∶85）为流动相,检测波长349 nm。结果：不同产地的紫花地丁中秦皮乙素的含量有较大差异,其中河北产紫花地丁中秦皮乙素的含量较高,但不稳定;陕西、河南产紫花地丁中秦皮乙素的含量较稳定。结论：紫花地丁中秦皮乙素的含量随地域差异变化较大。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.