何首乌多糖对免疫功能低下小鼠的免疫保护作用

目的:探讨何首乌多糖(PPM)对免疫功能低下小鼠的免疫保护作用。方法:小鼠腹腔注射环磷酰胺建立小鼠免疫功能低下模型后,灌胃PPM 0.4,0.8,1.6 g.kg-1,qd,连续给药10 d,另设模型对照组和正常对照组。采用显微镜观察腹腔巨噬细胞的吞噬百分率、吞噬指数和T淋巴细胞酯酶阳性率;用分光光度测定血清溶血素含量;用3H-TdR掺入法测定脾细胞淋巴细胞增殖率。结果:PPM能显著拮抗环磷酰胺所致免疫功能低下小鼠的免疫器官重量减轻和白细胞数量减少,明显增加小鼠腹腔巨噬细胞的吞噬率及吞噬指数,显著增加血清溶血素含量,且能明显促进T淋巴细胞酯酶阳性率和ConA诱导的脾T淋巴细胞增殖反应。结论:何首乌多糖对免疫功能低小鼠的免疫功能有明显的增强作用。     

山茶花总黄酮抗肿瘤作用的实验研究

目的研究山茶花总黄酮(TFC)对小鼠H22肝癌移植瘤生长的抑制作用及其机制。方法建立小鼠H22肝癌移植瘤模型,将60只接种H22肝癌细胞悬液的昆明小鼠随机分为6组:正常组、模型对照组、阳性对照组(环磷酰胺)、TFC高、中、低剂量组,给药12d,末次给药后1h处死全部小鼠,称小鼠肿瘤重量,观察TFC对H22肝癌移植瘤生长的抑制作用,及其对小鼠免疫器官、巨噬细胞吞噬、淋巴细胞转化功能的影响,另取60只小鼠,分组造模同上,观察小鼠给药后存活时间的变化。结果 TFC高、中剂量组可明显抑制瘤体重量;高、中剂量组可明显增加小鼠脾和胸腺指数,且高、中剂量组能显著提高巨噬细胞吞噬功能和淋巴细胞转化能力;高、中剂量组可明显延长小鼠的存活时间。结论 TFC可能通过调节机体免疫功能来发挥抗肿瘤作用。     

决明子蒽醌苷对小鼠免疫功能的调节作用

目的研究决明子蒽醌苷（SCAG）体外给药对小鼠细胞免疫功能的影响。方法将小鼠淋巴细胞或巨噬细胞与不同浓度的SCAG共培养，用二甲氧唑黄（XTT）法测定T及B淋巴细胞的增殖能力、对混合淋巴细胞反应（MLR）的影响、对丝裂霉素C所致淋巴细胞增殖抑制的拮抗作用，用中性红染色法观察SCAG对巨噬细胞吞噬功能、自然杀伤（NK）细胞活性、小鼠脾细胞分泌肿瘤坏死因子（TNF）活性的影响。结果SCAG体外给药可明显促进小鼠T及B淋巴细胞的增殖，增强巨噬细胞吞噬中性红的能力，提高NK细胞活性及分泌TNF活性，并可促进MLR，拮抗丝裂霉素C对淋巴细胞增殖的抑制作用。结论SCAG体外给药可显著增强小鼠细胞免疫功能。     

薏苡仁水提液对免疫抑制小鼠免疫功能的影响

目的探讨薏苡仁水提液对免疫功能低下小鼠的免疫调节作用。方法先给予小鼠腹腔注射环磷酰胺以建立小鼠免疫功能低下模型,然后将薏苡仁水提液按10、5和2.5 g.kg-1三种剂量连续给三组小鼠灌胃10 d,观察薏苡仁水提液对免疫低下小鼠免疫器官重量指数、白细胞数量、腹腔巨噬细胞吞噬率与吞噬指数、T淋巴细胞酯酶阳性率和血清溶血素HC50值等实验指标的影响。结果薏苡仁水提液能显著拮抗环磷酰胺所致免疫功能低下小鼠的免疫器官重量减轻和白细胞数量减少,明显增加小鼠腹腔巨噬细胞的吞噬百分率及吞噬指数,显著增加血清溶血素含量,且能明显促进T淋巴细胞酯酶阳性率。结论薏苡仁水提液对机体免疫功能具有较好的增强作用。表现为体液免疫、细胞免疫和非特异免疫功能的改变。     

龟叶草对小鼠免疫调控作用初步观察

对龟叶草水煎液进行免疫调控作用的实验观察。结果表明．灌胃给药与腹腔注射给药均能抑制小鼠碳粒廓清速率。腹腔给药对二硝基氟苯所致小鼠迟发型超敏反应有较强的抑制作用；对体外淋巴细胞转化试验有明显的抑制作用；对溶血素的形成略有增高作用。这些结果表明该制剂可能对免疫功能具有一定的调控作用。     

用HPLC法测定不同皮质功能状态时小鼠肾上腺的Vit C含量

本文采用高效液相电化学检测法测定正常小鼠及用促肾上腺皮质激素和地塞米松分别造成肾上腺皮质功能亢进和低下状态小鼠的肾上腺组织中维生素C(Vit C)的含量。结果提示,用该方法能较准确测出小鼠肾上腺的VitC含量。当皮质功能亢进时,小鼠肾上腺的VitC含量提高,皮质功能低下时,VitC含量减少。     

茶多酚对D-半乳糖致衰老小鼠免疫功能的调节作用

目的:观察茶多酚对D-半乳糖致衰老小鼠免疫功能的调节作用。方法:昆明种小鼠随机分为正常对照组、衰老模型组和茶多酚低、中、高剂量模型给药组,3个剂量组每天分别按1.0,2.0,4.0g.kg-1.d-1灌胃,正常对照组和模型组均灌胃等量生理盐水溶液,连续30d。计算胸腺与脾脏指数,测定淋巴细胞转化能力、小鼠腹腔巨噬细胞吞噬功能和外周血IL-2水平。结果:D-半乳糖致衰老小鼠免疫功能明显下降,表现为胸腺与脾脏指数、淋巴细胞转化能力、腹腔巨噬细胞吞噬功能、外周血IL-2含量均明显降低(P<0.05,P<0.01);茶多酚能提高模型小鼠胸腺与脾脏指数、淋巴细胞转化能力、腹腔巨噬细胞吞噬功能和外周血IL-2含量(P<0.05,P<0.01)。结论:茶多酚明显增强D-半乳糖致衰老小鼠的免疫机能,是一种免疫调节剂。     

萘丁美酮对小鼠免疫功能的影响

目的 :研究萘丁美酮对小鼠免疫功能的影响。方法 :小鼠灌胃萘丁美酮后 ,分别观察胸腺、脾脏重量 ,腹腔巨噬细胞吞噬功能和游走功能 ,体内PHA诱导的淋巴细胞转化。结果 :萘丁美酮能明显减轻小鼠胸腺和脾脏重量 ,降低小鼠腹腔巨噬细胞吞噬游走功能 ,抑制PHA诱导的小鼠淋巴细胞转化。结论 :萘丁美酮对小鼠细胞免疫功能有明显的抑制作用。     

乳清提取物对小鼠免疫功能影响及抗癌作用的初步研究

目的观察豆乳清中活性物质对小鼠免疫功能的影响及抗癌活性。方法乳清活性物质经灌胃或腹腔注射给药后 ,测定各组小鼠的胸腺指数、脾指数、巨噬细胞吞噬功能及抗癌效应。结果灌胃给药一定剂量可明显增加小鼠胸腺和脾指数 (P <0 .0 1 ) ,与对照组比较其吞噬百分率、吞噬指数差异显著 (P <0 .0 1 ) ;腹腔注射给药对荷U1 4肿瘤和荷HepA肿瘤小鼠的肿瘤生长有一定的抑制作用 ,抑瘤率以HepA更为显著。结论乳清活性物质具有一定的免疫增强功能和抗癌效应     

灵芝精粉对小鼠免疫功能的影响

目的 观察灵芝精粉对免疫功能的影响。方法 灵芝精粉两种工艺制品，两种剂量胃内给药(ig)于昆明小鼠，给药15d，检测免疫功能各参数。结果 灵芝精粉可明显增强小鼠脾淋巴细胞的转化能力及体液免疫能力；明显抑制由SRPC引起的DTH反应，增强腹腔巨噬细胞吞噬功能；对网状内皮系统功能无明显影响。结论 灵芝精粉具有一定的免疫调节作用。     

10-羟基-2-癸烯酸对小鼠T淋巴细胞及其亚型和白介素2产生的影响

观察了10-羟基-2-癸烯酸（10-HDA）对小鼠#FST#FK淋巴细胞及其亚型和白介素2产生的影响. 结果表明，10-HDA 1, 5, 25 mg·kg^-1·d^-1 ip, 5 d可拮抗环磷酰胺100 mg·kg^-1对小鼠迟发型超敏反应（DTH）的抑制作用. 体外给药，10-HDA可促进刀豆球蛋白A诱导的T淋巴细胞增殖反应；促进小鼠脾细胞产生白介素2. 采用单克隆抗体间接免疫荧?光法证明10-HDA可增加小鼠胸腺L₃T₄⁺细胞数，而对Lyt-2⁺细胞无明显影响. 结果提示10-HDA可调节T淋巴细胞参与的免疫反应.     

Modification of immune response by cinnarizine

Effects of cinnarizine on immune response in mice were investigated. Mice were orally administered with cinnarizine and were immunized with sheep red blood cells (SRBC) intravenously. Numbers of plaque forming cells (PFC) to SRBC in spleen of these mice were assayed and delayed-type hypersensitivity (DTH) response to SRBC was measured. 1) PFC response in immunization with 5 X 10(6) cells/mouse of SRBC was enhanced by administration of 25 mg/kg of cinnarizine, while the response in immunization with 5 X 10(8) cells/mouse was suppressed by 25 to 200 mg/kg of cinnarizine. 2) From study on timing of administration, suppression of PFC response by 6.25 to 200 mg/kg of cinnarizine was observed at 24 hr. after the immunization. 3) 12.5 to 200 mg/kg of cinnarizine suppressed polyclonal B cell activation induced by lipopolysaccharide (LPS). 4) Colchicine induced suppressor T cell inactivation was prevented by administration of 50 mg/kg of cinnarizine and it was suggested that cinnarizine may induce suppressor T cells from the study of adoptive cell transfer system. 5) 50 mg/kg of cinnarizine showed the suppression of DTH response in expression phase, but not in induction phase. It was concluded that immune responses in mice were modified by cinnarizine.     

脑室注射N-甲基-D-门冬氨酸对小鼠学习记忆的影响

采用一次性被动回避性条件反射方法,给小鼠icvN-甲基-D-门冬氨酸(NMDA)10,1和0.1 ng。结果表明:对正常小鼠,在跳台法中NMDA 10 ng能明显增强记忆巩固过程,在避暗法中NMDA 1 ng能显著增强记忆再现过程;在两种方法中,NMDA 1 ng均能显著改善乙醇及亚硝酸钠所致记忆障碍;而NMDA对记忆获得过程无影响。进一步研究表明,NMDA改善记忆的作用可被其受体特异性拮抗剂AP5所拮抗。结合以前报道和电生理研究结果提示,谷氨酸受体NMDA亚型在记忆过程中起着重要的作用,脑室给药作用的环节主要是影响记忆巩固和再现过程。     

牛膝多糖对小鼠红细胞免疫功能的影响

目的　探讨牛膝多糖 (ABP)对正常及免疫低下小鼠红细胞免疫功能的影响。方法　ABP经腹腔注射给药后 ,测定各组小鼠外周血中红细胞C3b受体及红细胞粘附免疫复合物花环结合率的变化。结果　ABP 6 0mg·kg-1·d-1,10d能显著提高正常及免疫低下小鼠红细胞C3b受体花环结合率和红细胞粘附免疫复合物花环结合率。结论　ABP是一种免疫调节剂 ,能提高正常及免疫低下小鼠的红细胞免疫功能     

甘草次酸钠口服给药的抗炎及免疫调节作用

ＳＧＡ多次灌胃使巴豆油诱发的小鼠耳肿减轻，并抑制佐剂性关节炎大鼠炎足肿胀、滑膜增生、血管翳形成及关节软骨破坏；使正常小鼠Ｔ淋巴细胞比率升高，但使佐剂性关节炎大鼠异常升高的Ｔ淋巴细胞比率降低，呈现双向免疫调节作用。     

脑室注射N-甲基-D-门冬氨酸对小鼠学习记忆的影响

采用一次性被动回避性条件反射方法,给小鼠icv N-甲基-D-门冬氨酸(NMDA)10,1和0.1 ng。结果表明:对正常小鼠,在跳台法中NMDA 10 ng能明显增强记忆巩固过程,在避暗法中NMDA 1 ng能显著增强记忆再现过程;在两种方法中,NMDA 1 ng均能显著改善乙醇及亚硝酸钠所致记忆障碍;而NMDA对记忆获得过程无影响。进一步研究表明,NMDA改善记忆的作用可被其受体特异性拮抗剂AP5所拮抗。结合以前报道和电生理研究结果提示,谷氨酸受体NMDA亚型在记忆过程中起着重要的作用,脑室给药作用的环节主要是影响记忆巩固和再现过程。     

关于抗肿瘤药物的研究Ⅸ.N-甲酰溶肉瘤素的一些药理作用

抗肿瘤药N-甲酰溶肉瘤素(简称N-甲)的毒性低.小鼠于每天口服25-150毫克/公斤连续10天,游泳耐力不减,肝脏破坏戊巴比妥钠功能正常.小鼠于腹腔注射N-甲10-20毫克/公斤/天,连续10天,或150毫克/公斤一次,对因戊巴比妥钠产生的睡眠时间无改变.大鼠于每天腹腔注射N-甲10毫克/公斤,连续10天,其利尿功能,肾上腺内维生素C含量及微血管渗进性均无明显改变.麻醉兔于静脉注射N-甲10-40毫克/公斤后,血压亦无明显改变.当N-甲20毫克/公斤/天,连续10天时,于注射后5天,大鼠利尿功能受到抑制,但于停药后3天逐渐恢复正常.大鼠于腹腔注射N-甲80毫克/公斤后2小时,肾上腺内维生素C含量明显下降,微血管渗透性显著增加.     

Influence of the Oral Administration of Excess Copper on the Immune Response

We have studied the influence of the oral administration ofexcess copper (Cu) on the immune response. With this aim, micemaintained on standard laboratory diet received 50, 100, 200,or 300 ppm of Cu as copper sulfate in the drinking water during3 to 10 weeks. Inhibition of the proliferative response to concanavalinA was observed in mice exposed to 100 ppm of Cu for 8 weeksand to 200 ppm of Cu for either 3 or 8 weeks. Conversely, asignificant increase in the proliferative response to Eschenchiacolilipopolysaccharide (LPS) was observed in mice exposed to50 or 100 ppm of Cu for 3 weeks. However, the response to LPSwas also significantly inhibited following prolonged Cu administration.In contrast, mice exposed to low or high Cu doses during shortor long periods showed increased production of autoantibodiesdirected to bromelain-treated mouse erythrocytes. The DTH responseto sheep red blood cells was not modified following short-termadministration of 100 ppm of Cu, but was depressed after prolongedexposure to this dose of the metal. Significant inhibition ofthe DTH response was observed in mice exposed to 300 ppm ofCu for 5 or 10 weeks. Thus, oral administration of excess Cualtered the immune response in a fashion related to the doseand duration of treatment     

Study of active substances involved in skin dysfunction induced by crowding stress. I. Effect of crowding and isolation on some physiological variables,skin function and skin blood perfusion in hairless mice

The effects of five levels of population density on various organs, the neuroendocrine system, skin function, skin blood perfusion, and blood parameters were studied in the hairless mouse. Skin barrier recovery was evaluated by measuring transepidermal water loss after tape stripping. Blood perfusion was measured by means of a laser Doppler imaging technique. The effect of a parasympathetic nerve stimulator, carpronium chloride, on skin function in the crowded animal model was also examined. A 7 d crowding (10, 15, 20 mice/cage) significantly increased the levels of corticosterone, catecholamines (norepinephrine, epinephrine and dopamine), glucose and serum lactate dehydrogenase activity in circulating blood, induced atrophy of kidney, ovary and thymus and hypertrophy of adrenal glands, and decreased body weight gain in comparison with the control (5 mice/cage). Crowding also increased epidermal thickness and epidermal proliferative activity, and decreased corneocyte size, rate of barrier recovery and skin blood perfusion. Most of these changes became more marked with increasing population density and/or longer exposure to a crowded environment. Isolation (1 mouse/cage) increased the level of norepinephrine and rate of skin blood perfusion, and significantly delayed barrier recovery. Repeated topical applications of carpronium chloride for 7 d improved the changes in skin blood perfusion, barrier recovery, kidney and ovary, and epidermal morphology induced by crowding. The crowded animal model could be useful for quantifying objectively the influence of crowded environment-induced stress on cutaneous function and blood perfusion.     

萘普待因的一般药理试验

应用ＩＤＣ－１型多用生理电信号实时分析仪分别测定了萘普待因三种剂量（１４．９、２９．７、４４．６ｍｇ·ｋｇ－１,分别相当于人用剂量１、２、３倍）。ｉｇ给药１２０ｍｉｎ内药物对大鼠的心电、血压均无明显影响（Ｐ＞０．０５）。低剂量对大鼠的呼吸也无影响。中、高剂量给药３０ｍｉｎ后,大鼠的呼吸减慢加深,６０ｍｉｎ后基本恢复正常。观察各剂量组给药后的一般状况,未见异常。药物对小鼠自发活动的影响仅表现在高剂量组（８５．８ｍｇ·ｋｇ－１,相当于人用剂量的４倍）,其小鼠自发活动次数及总强度与生理盐水（ＮＳ）组相比明显减弱（Ｐ＜０．０１）,其余剂量组（２１．５、４２．９、６４．４ｍｇ·ｋｇ－１,分别相当于人用剂量的１、２、３倍）ｉｇ给药后,１２０ｍｉｎ内小鼠的自发活动无明显改变。