慢性阻塞性肺疾病病人外周血T淋巴细胞的检测及意义

目的检测慢性阻塞性肺疾病(COPD)患者体内各类T淋巴细胞的亚群,了解COPD患者的细胞免疫功能状态。方法采用免疫荧光双标记法和流式细胞仪检测COPD患者外周血中的T淋巴细胞及其亚群。结果与健康对照组相比较,急性期COPD组患者CD3+T细胞及CD4+T细胞亚群下降,CD8+T亚群细胞升高,导致CD4+/CD8+比值下降并倒置;CD4+CD45RA+亚群、CD4+CD45RA+/CD4+CD45RO+比值下降,CD4+CD45RO+亚群上升;CD8+CD45RA+亚群、CD8+CD45RO+亚群和CD8+CD45RA+/CD8+CD45RO+比值均有所上升;自身免疫性疾病有关的CD4+CD28+亚群下降。结论 COPD患者体内存在细胞免疫功能的紊乱。     

乙型肝炎患者外周血T淋巴细胞亚群的变化

目的研究乙型肝炎患者外周血T淋巴细胞亚群变化及其临床意义.方法对140例乙型肝炎患者和64例健康者,采用特异性荧光抗体标记、流式细胞仪检测T淋巴细胞亚群的改变.结果与正常对照组比较,慢性肝炎、肝炎肝硬变、慢性重型肝炎患者外周血CD3+、CD4+、CD8+及CD4+/CD8+比值均有显著性下降(P＜0.05),以肝炎肝硬变下降最显著,急性乙型肝炎T细胞亚群数量和比值变化不明显(P＞0.05).HBV DNA(+)与HBV DNA(-)患者外周血T细胞亚群数量和比值变化比较无显著性差异(P＞0.05).结论乙型肝炎病毒感染后机体T淋巴细胞功能低下主要见于慢性病毒感染状态,其下降程度与病毒复制水平无明显关系.     

老年SD大鼠脾脏和外周血T淋巴细胞亚群的变化

目的探讨SD老年大鼠脾脏和外周血T淋巴细胞亚群的变化。方法流式细胞仪检测老年大鼠(10只)和青年鼠(5只)脾脏和血液T淋巴细胞亚群的变化。结果 SD老年大鼠脾脏和外周血T淋巴细胞亚群阳性率的变化相一致,CD3+、CD4+、CD8+细胞阳性率均低于青年组水平,且CD4细胞阳性率均明显低于青年组(P<0.05);CD4+/CD8+比值均低于青年组,在脾脏CD4+/CD8+比值与青年组相比具有显著性差异(P<0.05)。结论 SD老年大鼠脾脏和外周血T胞亚群阳性率均减少,且CD4+/CD8+比值明显降低,提示SD老年大鼠免疫功能的衰退和紊乱与T细胞密切相关。     

特发性膜性肾病患者调节性T细胞和B淋巴细胞的变化及其临床意义

目的:观察特发性膜性肾病(IMN)患者外周血调节性T细胞、B淋巴细胞亚群以及肾组织中B淋巴细胞浸润分布的变化,探讨与膜性肾病(MN)发病机制及临床表现的关系. 方法:66例(男性45例,女性21例,年龄15～71岁)经肾活检确诊为MN,排除各种继发因素,近期未使用大剂量免疫抑制剂的IMN患者,流式细胞仪测定外周血调节性T细胞(CD4+CD25+Foxp3+Treg细胞)、B淋巴细胞(CD20+细胞)和T淋巴细胞亚群(CD3+、CD4+、CD8+细胞)的计数;另采用免疫组织化学方法检测患者肾组织中B淋巴细胞数量及分布.40例年龄、性别匹配的健康志愿者作为对照.根据患者尿蛋白水平,将其分为大量蛋白尿组(尿蛋白定量≥3.5 g/d)及非大量蛋白尿组(尿蛋白定量＜3.5g/d).比较IMN患者外周血调节性T细胞,B淋巴细胞及T淋巴细胞亚群与正常对照的差别,以及IMN患者中大量蛋白尿者和非大量蛋白尿者之间上述淋巴细胞亚群水平的差异及其可能的临床意义. 结果:(1)IMN患者外周血调节性T细胞计数较正常对照组显著降低[(23.2±10.7) cells/μl vs (31.4±7.29) cells/μl,P<0.01].(2)与正常对照组比较,IMN患者外周血B淋巴细胞计数显著升高[(208±111) cells/μl vs (137±64) cells/μl,P<0.01].(3)IMN患者和正常对照组相比,其CD4+细胞计数有升高趋势[(715±267) cells/μl vs (657±214) cells/μl,P>0.05],CD8+细胞计数有降低趋势[(393±178) cells/μl vs (464±200) cells/μl,P>0.05],而CD4+/CD8+比值较正常对照显著升高(2.04±0.92 vs 1.52±0.48,P<0.01).(4)大量蛋白尿组B淋巴细胞计数明显高于非大量蛋白尿组[(246±117) cells/μl vs (186±108) cells/μl,P<0.05],但两组之间调节性T细胞、CD4+细胞、CD8+细胞计数及CD4+/CD8+比值均无统计学差异(P>0.05).(5)IMN患者肾组织中B淋巴细胞浸润数量较正常对照升高,伴肾组织B淋巴细胞灶性聚集者其尿NAG酶水平升高. 结论:IMN患者表现出调节性T细胞水平降低,B淋巴细胞水平上调和CD4+/CD8+细胞比值的偏移.外周血B淋巴细胞水平升高与IMN患者大量蛋白尿关系密切,肾组织中B淋巴细胞浸润数目较正常对照升高,存在局灶B淋巴细胞聚集的患者小管间质急性损伤加重.对IMN患者B淋巴细胞的数目和组织分布进行观察,有可能为监测病情、指导治疗提供帮助.     

原发性肝癌和乙型肝炎肝硬化患者外周血淋巴细胞凋亡率、T细胞亚群和NK细胞的水平变化和临床意义

目的通过检测乙型肝炎肝硬化和合并乙型肝炎病毒感染的原发性肝细胞癌患者的外周血的粒细胞、单核细胞、NK细胞、T细胞及其亚群和淋巴细胞及其凋亡率,探讨两者在细胞免疫方面的差异.方法用Ficoll Hypaque离心法分离外周血单个核细胞(PBMNC),流式细胞仪检测外周血T细胞及其亚群、NK细胞和淋巴细胞、单核细胞和粒细胞,AnnexinV/FITCKit检测凋亡细胞.结果乙型肝炎肝硬化患者的外周血单核细胞、粒细胞、淋巴细胞、CD3-CD16 CD56 NK细胞、CD3 T细胞、CD3 CD4 T细胞、CD3 CD4 T细胞和CD4 CD8 T细胞比值,均与正常对照组无显著性差异(P＞0.05);但淋巴细胞凋亡率明显低于对照组(P＜0.01).原发性肝癌外周血CD3-CD16 CD56 NK细胞、单核细胞和CD4 /CD8 T细胞比值与肝硬化组和正常对照组比较,均无显著性差异(P＞0.05),而粒细胞明显升高(P＜0.05);CD3 T细胞、CD3 CD4 T细胞和CD3 CD8 T细胞均较另两组明显减少(P＜0.05),淋巴细胞及其凋亡率均明显低于另两组(P＜0.01).结论乙型肝炎肝硬化患者的外周血细胞免疫只发生不显著的变化,但淋巴细胞的凋亡率明显降低.原发性肝癌外周血的细胞免疫和淋巴细胞凋亡率均明显低下.     

严重肝病患者并发感染性休克外周血T淋巴细胞亚群的分析

目的探讨并发感染性休克的肝病患者外周血T淋巴细胞亚群的变化。方法在42例并发感染性休克的慢性乙型肝炎患者,取外周血,采用特异性荧光抗体标记、BD流式细胞仪检测其T淋巴细胞亚群。结果慢性乙型肝炎并发感染性休克患者T淋巴细胞亚群表现为CD3 、CD4 、CD8 T淋巴细胞及CD4 /CD8 T比值明显下降(P<0.01),与无并发症组比较,CD3 、CD4 T淋巴细胞均明显下降(P<0.05),但CD8 T淋巴细胞无明显变化(P>0.05),导致CD4 /CD8 比值明显降低(P<0.05)。结论慢性乙型肝炎并发感染性休克患者T淋巴细胞亚群比无并发症组更紊乱,免疫功能更低下。     

原发性胆汁性肝硬化T淋巴细胞亚群分析

目的 探讨原发性胆汁性肝硬化(PBC)患者的T淋巴细胞亚群及共刺激信号表达的特点及临床意义.方法 以未经治疗的98例PBC患者为研究组,性别、年龄匹配的健康人30名作为对照组.以流式细胞仪技术检测外周血淋巴细胞亚群以及T淋巴细胞表面的共刺激信号CD28.结果 PBC与对照组T细胞亚群差异有统计学意义:PBC组CD4+T淋巴细胞升高,CD8+T淋巴细胞下降,CD4+/CD8+比值上升(P<0.05);CD4+CD28-T细胞和CD8+CD28-T细胞明显增加(P<0.05).结论 PBC存在免疫调节功能的异常,其中CD28的表达明显减少;CD8+CD28-的细胞群可能在PBC中有一定的免疫调节作用.     

系统性红斑狼疮患者外周血CD4+ CD8+和CD22+淋巴细胞活化分子CD69的表达

目的探讨系统性红斑狼疮(SLE)患者外周血淋巴细胞(PBL) T细胞(CD4+、CD8+)和B细胞(CD22+)活化分子CD69的表达.方法应用双染色流式细胞术检测CD4、CD8、和CD22细胞亚群CD69分子;在植物凝集素(PHA)刺激后20 h淋巴细胞亚群CD69分子的表达.结果①SLE患者PBMC的CD69分子活动期高于静止期(P＜0.001)和正常对照组(P＜0.01)的表达,SLE静止期患者与正常对照组CD69表达差异无显著性(P＞0.05).②进一步分析CD4+、CD8+和CD22+淋巴细胞亚群的CD69的表达,其中,SLE活动期患者CD4+细胞的CD69表达显著高于静止期(P＜0.001)和正常对照组(P＜0.01)的表达,SLE静止期患者与正常对照组CD69表达差异无显著性(P＞0.05);CD8+细胞活动期高于静止期患者(P＜0.05),其余组间差异无显著性(P＞0.05);CD22+B细胞各组间差异无显著性.③PHA刺激20 h后,CD4+、CD22+B细胞的CD69表达,活动期显著高于静止期患者和正常对照组(P＜0.01).结论 SLE患者外周血CD4+T细胞和CD22+B细胞存在着异常的活化,这种淋巴细胞的异常活化是SLE重要的发病机制之一.     

急性心肌梗死患者外周血T淋巴细胞及其亚群与C-反应蛋白的相关性

目的:研究急性心肌梗死(AMI)患者外周血T淋巴细胞及其亚群和血清C反应蛋白(CRP)的变化,探讨两者之间的相关性。方法:采用流式细胞仪技术测定33例AMI患者外周血T淋巴细胞及其亚群,并与对照组的30例比较;采用免疫散射比浊法测定33例AMI患者血清CRP的系列变化值,取其峰值。结果:AMI患者外周血CD3+和CD4+显著低于对照组(P<0.01),CD8+无明显改变(P>0.05),CD4+/CD8+比值明显低于对照组(P<0.01);CRP峰值与CD4+/CD8+比值呈显著的负相关(r=-0.731,P<0.01)。结论:AMI患者细胞免疫功能受到抑制,T细胞亚群的变化与炎症因子CRP有相关性,炎症因子及免疫机制可能都是影响AMI患者病情进展的重要因素。     

病毒性心肌炎患者外周血T淋巴细胞亚群及B淋巴细胞的研究

目的: 测定不同时期病毒性心肌炎(VMC)患者T淋巴细胞亚群及B淋巴细胞的数量,并同时检测患者血清心肌损害标志物心肌肌钙蛋白I(cTnI)的水平,探讨其在VMC发病机制中的作用。方法: 采用流式细胞术检测不同时期VMC患者(n=126)外周血CD4+CD45RA+和CD4+CD45RO+以及CD19+的表达,并设正常人群作为对照组(n=50)。结果: 急性期（<3个月）患者CD4+CD45RA+和CD4+CD45RO+以及CD19+与对照组相比差异显著。<1个月以CD4+CD45RA+和CD4+CD45RO+为著（P<0.01;P<0.01）。病程<7 d的患者与对照组相比,CD4+CD45RA+值的降低具有显著性(P<0.01);CD4+CD45RO+值下降明显(P<0.01)。而病程7~14 d、14~21 d及21~30 d的患者CD4+CD45RA+值、CD4+CD45RO+值及CD19+值与对照组相比差异均有统计学意义,CD4+CD45RO+值恢复较快。CD4+CD45RA+/CD4+CD45RO+在发病14 d内的患者与对照组差异无统计学意义;而14~30 d的患者与对照组相比差异有显著性。发病1~3个月患者以CD19+为著（P<0.01）。急性期CD4+CD45RA+/CD4+CD45RO+细胞的比例失衡。恢复期（3~12个月）患者CD4+CD45RA+和CD4+CD45RO+以及CD19+、CD4+CD45RA+/CD4+CD45RO+细胞的比例与对照组相比差异无显著性。结论: VMC患者感染初期（<1个月）以T淋巴细胞亚群异常为主,感染后期（1~3个月）以B淋巴细胞异常较为显著,以上两时期均存在CD4+CD45RA+/ CD4+CD45RO+细胞比例失衡。恢复期（3~12个月）T淋巴细胞及B淋巴细胞逐渐恢复至正常。     

T lymphocyte subsets in Mediterranean spotted fever

Several studies have previously suggested the possible role of a T lymphocyte suppressor population in infections by species of the genus Rickettsia. In 15 patients with Mediterranean spotted fever (MSF), we quantified, during the acute and convalescent phases of the disease, the peripheral blood lymphocyte populations using monoclonal antibodies that recognize CD3+, CD4+, CD8+, CD38+ and CD20+ cells. In three cases a reversal in the normal ratio of T lymphocyte helper-inducer/suppressor-cytotoxic subsets was detected lasting, in two of them, up to the fifth week of the disease. This disturbance was always weak and lacked clinical significance.     

Quantitation of T lymphocyte subsets helps to distinguish dengue hemorrhagic fever from classic dengue fever during the acute febrile stage

Activation of immunoregulatory T lymphocyte subsets has been observed in dengue viral infection, being more evident in dengue hemorrhagic fever (DHF) than in classical dengue fever (DF). There are, however, as yet no well-defined host markers to determine which patients with dengue viral infection will develop severe complications during the acute febrile stage of the disease. A study was performed to compare the cellular immune status in DHF, DF and non-dengue viral infections (NDF) in order to determine the value of these parameters in distinguishing DHF from classic DF and other viral infections during the acute febrile stage of the disease. This study involved 109 febrile patients admitted because of suspected DHF. Fifty patients were serologically confirmed cases of dengue infection, of which 25 had grade 1 or 2 DHF. There was a reduction in total T (CD3), CD4 and CD8 cells in DHF and demonstrated that a low level of CD3, CD4, CD8 and CD5 cells discriminated DHF from DF patients during the febrile stage of the illness. In contrast, B (CD19) cells and natural killer (NK) cells did not appear to be discriminatory in this study. Receiver operating characteristic (ROC) curve analysis showed that a combination of CD3 cell of < or = 45% and CD5 cell of < or = 55% was the best marker to identify DHF patients (sensitivity = 84% and specificity = 52% for CD3 cell of < or = 45%; sensitivity = 92% and specificity = 71% for CD5 cell of < or = 55%). CD4 cell of < or = 25% and CD8 cell < or = 30% were equally good in discriminating DHF from DF patients. On the other hand, the ROC curves indicated no clear difference between the immunoregulatory cell counts in DF from NDF Lymphopenia, atypical lymphocytosis and thrombocytopenia were significantly more evident in dengue compared to non-dengue infection but did not appear to be discriminatory among DHF and DF patients. The reduction in CD3, CD4, CD8, CD5 cells correlated with the degree of thrombocytopenia in DHF (p < 0.05) which suggests that these cells probably participate in a common pathogenetic mechanism.     

Abnormalities of T cell immunoregulation in hemorrhagic fever with renal syndrome

The functions of spontaneous suppressor T cells and T lymphocyte subsets in patients with hemorrhagic fever with renal syndrome were compared. In the early stages of disease, decreased activity of spontaneous suppressor T cells was concurrent with increased numbers of CD8+ cells and a reversed CD4:CD8 ratio. These changes were related to abnormalities in serum C3 level and circulating immune complexes. In the recovery stages of the illness, spontaneous suppressor T cell activity and T cell subsets returned to normal levels.     

CD178在肾综合征出血热患者外周血T细胞亚群表达的研究

为研究 CD1 78在肾综合征出血热 (HFRS)患者外周血 T细胞亚群的表达及意义 ,应用荧光抗体标记和流式细胞仪技术分析 CD1 78(Fas配体 )在 HFRS患者及健康人群 (对照组 )外周血 CD 4、CD 8T细胞表达的水平。结果 HFRS患者发热期组和多尿期组外周血 CD 4T淋巴细胞比例与正常对照组比较差异无显著性 ;而 CD 8T细胞明显增加 (P<0 .0 1) ;CD 4/CD 8比值明显下降 (P<0 .0 5 ) ;CD 4、CD1 78 T淋巴细胞和 CD 8、CD1 78 T淋巴细胞皆显著增高 (P<0 .0 5及 P<0 .0 0 1)。HFRS患者发热组和多尿组 CD 8T细胞的 CD1 78的表达率分别为 2 2 .18%和2 9.92 % ,而 CD 4T细胞的 CD1 78表达率分别为 4 .80 %和 5 .2 5 %。认为 HFRS的发病过程中 CD1 78主要表达于CD 8T淋巴细胞亚群 ,且发病早期和末期均有高表达     

Kinetics of lymphocyte subpopulations in dengue hemorrhagic fever/dengue shock syndrome

A kinetic study of lymphocyte subpopulations was performed in 61 dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) patients aged 8 months to 12 years and in 59 age-matched normal controls. There were 36 patients in grade 2 and 25 patients in grade 3 of the disease severity. The studies were performed on febrile stage, the day of subsidence of fever or shock stage, 3 subsequent days after subsidence of fever or shock, and once on the recovery stage (approximately 14-18 days after subsidence of fever or shock). The study revealed that the absolute total lymphocytes, CD3+, CD4+, CD8+ and HNK-1+ cells were decreased on febrile stage and their lowest values were noted on the first day of subsidence of fever or shock, while B1+ cells were in the normal range. Thereafter, all lymphocyte subpopulations were increased. The total lymphocytes, B1+ and CD8+ cells were rapidly increased and were above normal value on day 2 after subsidence of fever or shock (early convalescence), then gradually declined to the normal range. In contrast, CD3+, CD4+ and HNK-1+ cells were increased gradually and reached their normal values on day 2 after subsidence of fever or shock. The T4:T8 ratio began to reverse on the day of subsidence of fever or shock, reached its peak on day 2 after shock and returned to normal ratio rapidly thereafter. Thus, the absolute lymphopenia on the day of shock was due to the decrement or T cells (both CD4+ and CD8+ cells) and HNK-1+ cells.(ABSTRACT TRUNCATED AT 250 WORDS)     

Effects of severe acute respiratory syndrome (SARS) coronavirus infection on peripheral blood lymphocytes and their subsets.

INTRODUCTION: Severe acute respiratory syndrome (SARS) caused large outbreaks of atypical pneumonia in 2003, with the largest localized outbreak occurring in Beijing, China. Lymphopenia was prominent amongst the laboratory abnormalities reported in acute SARS. METHODS: The effect of SARS on peripheral blood lymphocytes and their subsets was examined in 271 SARS coronavirus-infected individuals. RESULTS: There was a significant decrease in the CD45+, CD3+, CD4+, CD8+, CD19+ and CD16+/56+ cell counts over the five weeks of the SARS illness although CD4+/CD8+ ratios did not change significantly. The lymphopenia was prolonged, reaching a nadir during days 7-9 in the second week of illness before returning towards normal after five weeks, with the lowest mean CD4+ cell count of 317 cellsx10(6)/L at day 7, and CD8+ cell count of 239 cellsx10(6)/L at day 8. Patients with more severe clinical illness, or patients who died, had significantly more profound CD4+ and CD8+ lymphopenia. DISCUSSION: Lymphopenia is a prominent part of SARS-CoV infection and lymphocyte counts may be useful in predicting the severity and clinical outcomes. Possible reasons for the SARS-associated lymphopenia may be direct infection of lymphocytes by SARS-CoV, lymphocyte sequestration in the lung or cytokine-mediated lymphocyte trafficking. There may also be immune-mediated lymphocyte destruction, bone marrow or thymus suppression, or apoptosis.     

TREC/KREC Levels and T and B Lymphocyte Subpopulations in COVID-19 Patients at Different Stages of the Disease

Background: T and B cell-mediated immunity can be assessed using T cell receptor excision circle (TREC) and Kappa-deleting recombination excision circle (KREC) analysis, respectively, and successful implementation of this method requires evaluation of the correlation between the TREC frequencies and T cell subsets as well as KREC levels and B lymphocyte subsets. The aim of the present study was to evaluate the correlation between the TREC/KREC concentrations and T/B lymphocyte subsets at different stages of COVID-19. Methods: We examined 33 patients in the acute stage of COVID-19 (including 8 patients with poor outcomes) and 33 COVID-19 survivors. TREC/KREC concentrations were measured using quantitative real-time PCR. T/B lymphocyte subsets were determined using flow cytometry. Results: Blood TREC and KREC levels were found to be significantly lower in the acute stage of COVID-19 compared to control values. Moreover, a zero blood TREC level was a predictor of a poor disease outcome. Reductions in CD3⁺CD4⁺CD45RO⁻CD62L⁻ and CD3⁺CD8⁺CD45RO⁻CD62L⁻ T cell counts (as well as in the main fractions of B1 and B2 B cells) indicated a favorable outcome in COVID-19 patients in the acute stage of the disease. Decreased CD3⁺CD4⁺CD45RO⁻CD62L⁺ and CD3⁺CD8⁺CD45RO⁻CD62L⁺ T cell frequencies and increased CD3⁺CD8⁺CD45RO⁻CD62L⁻ cell counts were found to indicate a poor outcome in patients with acute COVID-19. These patients were also found to have increased B1 cell counts while demonstrating no changes in B2 cell counts. The levels of effector T cell subsets an naïve B cells were normal in COVID-19 survivors. The most pronounced correlations between TREC/KREC levels and T/B cell subsets counts were observed in COVID-19 survivors: there were positive correlations with naïve T and B lymphocytes and negative correlations with central and effector memory T cell subsets. Conclusions: The assessment of correlations between TREC and T cell subsets as well as KREC levels and B cell subset counts in patients with acute COVID-19 and COVID-19 survivors has shown that blood concentrations of TREC and KREC are sensitive indicators of the stage of antigen-independent differentiation of adaptive immunity cells. The results of the TREC and KREC analysis correlated with the stages of COVID-19 and differed depending on the outcome of COVID-19.     

肾综合征出血热患者淋巴细胞亚群的动态变化

目的 研究肾综合征出血热(HFRS)患者病程不同阶段以及不同病情T、B、NK淋巴细胞亚群的变化,进一步揭示本病的发病机制.方法 入选22例确诊HFRS患者,其中轻型9例,中型13例,使用流式细胞技术检测发病1、4、12周时淋巴细胞亚群.56例健康献血员作为正常对照组.将疾病不同阶段与对照组及轻、中型患者之间进行比较分析.结果 在本研究观察的12周中,患者B细胞计数与对照组比较差异无统计学意义;而NK细胞计数起病1周时有显著下降(P<0.01),在4周时迅速恢复.本研究中HFRS患者CD4+T淋巴细胞计数与对照组比较差异无统计学意义,但在发病1、4周时记忆表型的CD+4CD45RA-T淋巴细胞比例增高(P<0.01),分别达到(64.1±17.5)%、(59.9±10.1)%,在12周时恢复正常.CD+4CD+28T淋巴细胞功能亚群无显著变化.CD+8T淋巴细胞计数在发病1、4周时有明显增高,12周恢复正常.CD+8CD-28T淋巴细胞计数有显著增高(P<0.05),且在轻型患者中这种变化更迅速、显著.与正常对照组相比,CD+38或HLA-DR+的CD+8T淋巴细胞激活亚群在发病1、4周时也有明显增高(P<0.01).结论 细胞免疫与HFRS有密切的关系,早期细胞毒效应T细胞及时有效的应答能清除病毒,减轻病情.     

脾栓塞对肝硬化患者外周血T淋巴细胞亚群的影响

目的 :研究部分脾栓塞治疗脾功能亢进对肝硬化患者T淋巴细胞亚群的影响。方法 :57例肝硬化并脾功能亢进患者 ,32例采用部分脾栓塞治疗 ,2 5例采用脾全切治疗 ,1 5例正常人作为对照 ,比较两组治疗前后外周血T淋巴细胞亚群的变化。结果 :肝硬化患者外周血T淋巴细胞 (CD3+ )、T辅助 /诱导淋巴细胞亚群 (CD4+ )、T辅助淋巴细胞 /T抑制淋巴细胞亚群 (CD4+ /CD8+ )明显低于正常人 (P <0 .0 1 ) ;脾栓塞组治疗前后CD3+ 、CD4+ 、CD8+ 、CD4+ /CD8+ 无明显差异 (P >0 .0 5) ;脾全切组治疗后CD3+ 、CD4+ 、CD4+ /CD8+ 均较治疗前及部分脾栓塞组治疗后明显降低 (P <0 .0 1 )。结论 :肝硬化患者外周血CD3+ 、CD4+ 、CD4+ /CD8+ 降低 ,部分脾栓塞治疗脾功能亢进 ,对肝硬化患者外周血T淋巴细胞亚群无明显影响 ,显著优于脾全切治疗     

Early CD69 expression on peripheral blood lymphocytes from children with dengue hemorrhagic fever.

Recent reports have demonstrated immune activation in dengue hemorrhagic fever (DHF) by cytokine and soluble receptor detection in blood. The goal of this study was to determine which cell types are activated and likely to be responsible for cytokine production. Whole blood specimens from 51 Thai children presenting within 72 h of fever onset and with detectable plasma dengue viral RNA were studied by flow cytometry. Absolute CD4 T cell, CD8 T cell, NK cell, and gammadelta T cell counts were decreased in children with DHF compared with those with dengue fever (DF) early in the course of illness. The percent of cells expressing CD69 was increased on CD8 T cells and NK cells in children who developed DHF more than in those with DF. These data directly demonstrate that cellular immune activation is present early in acute dengue and is related to disease severity.