共查询到20条相似文献,搜索用时 31 毫秒
1.
Kangmee Woo Amal K. Dutta Vishal Patel Charles Kresge Andrew P. Feranchak 《The Journal of physiology》2008,586(11):2779-2798
ATP in bile is a potent secretogogue, stimulating cholangiocyte Cl− and fluid secretion via binding to membrane P2 receptors, though the physiological stimuli involved in biliary ATP release are unknown. The goal of the present studies was to determine the potential role of fluid flow in biliary ATP release and secretion. In both human Mz-Cha-1 biliary cells and normal rat cholangiocyte monolayers, exposure to flow increased relative ATP release which was proportional to the shear stress. In parallel studies, shear was associated with an increase in [Ca2+ ]i and membrane Cl− permeability, which were both dependent on extracellular ATP and P2 receptor stimulation. Flow-stimulated ATP release was dependent on [Ca2+ ]i , exhibited desensitization with repetitive stimulation, and was regulated by PKCζ. In conclusion, both human and rat biliary cells exhibit flow-stimulated, PKCζ-dependent, ATP release, increases in [Ca2+ ]i and Cl− secretion. The finding that fluid flow can regulate membrane transport suggests that mechanosensitive ATP release may be a key regulator of biliary secretion and an important target to modulate bile flow in the treatment of cholestatic liver diseases. 相似文献
2.
G. D. S. Hirst N. J. Bramich N. Teramoto H. Suzuki† F. R. Edwards 《The Journal of physiology》2002,540(3):907-919
Regenerative potentials were initiated by depolarizing short segments of single bundles of circular muscle isolated from the gastric antrum of guinea-pigs. When changes in [Ca2+ ]i and membrane potential were recorded simultaneously, regenerative potentials were found to be associated with an increase in [Ca2+ ]i , with the increase starting after a minimum latency of about 1 s. Although the increase in [Ca2+ ]i was reduced by nifedipine, the amplitudes of the regenerative responses were little changed. Regenerative responses and associated changes in [Ca2+ ]i were abolished by loading the preparations with the Ca2+ chelator MAPTA-AM. Regenerative potentials were abolished by 2-aminoethoxydiphenyl borate (2APB), an inhibitor of IP3 induced Ca2+ release, by N -ethylamaleimide (NEM), an alkylating agent which blocks activation of G-proteins and were reduced in amplitude by two agents which block chloride (Cl− )-selective channels in many tissues. The observations suggest that membrane depolarization triggers IP3 formation. This causes Ca2+ release from intracellular stores which activates Ca2+ -dependent Cl− channels. 相似文献
3.
Rat peritoneal mast cells purified on a Percoll gradient were loaded with the fluorescent Ca2+ indicator fura-2 and were challenged with different concentrations of substance P (SP), and intracellular calcium concentrations ([Ca2+ ]i ) were measured by a spectrofluorometric assay. SP at 5 × 10−6 mol/1 and 10−5 mol/1 caused a significant histamine release with a significant increase in [Ca2+ ]i in a dose-dependent manner. However, SP at 10−8 -10−6 mol/1 did not induce either histamine release or increase in [Ca2+ ]i . Extracellular calcium at 0.9 mM inhibited the histamine release with a significant reduction of [Ca2+ ]i compared with that of the cells in a nominally calcium-free condition. These results indicate that the action of SP on rat mast cells relies upon [Ca2+ ]i to induce histamine release. 相似文献
4.
Pasi Tavi David G. Allen Perttu Niemelä† Olli Vuolteenaho‡ Matti Weckström† Håkan Westerblad 《The Journal of physiology》2003,551(1):5-12
Activation of the contractile machinery in skeletal muscle is initiated by the action-potential-induced release of Ca2+ from the sarcoplasmic reticulum (SR). Several proteins involved in SR Ca2+ release are affected by calmodulin kinase II (CaMKII)-induced phosphorylation in vitro , but the effect in the intact cell remains uncertain and is the focus of the present study. CaMKII inhibitory peptide or inactive control peptide was injected into single isolated fast-twitch fibres of mouse flexor digitorum brevis muscles, and the effect on free myoplasmic [Ca2+ ] ([Ca2+ ]i ) and force during different patterns of stimulation was measured. Injection of the inactive control peptide had no effect on any of the parameters measured. Conversely, injection of CaMKII inhibitory peptide decreased tetanic [Ca2+ ]i by ≈25 %, but had no significant effect on the rate of SR Ca2+ uptake or the force-[Ca2+ ]i relationship. Repeated tetanic stimulation resulted in increased tetanic [Ca2+ ]i , and this increase was smaller after CaMKII inhibition. In conclusion, CaMKII-induced phosphorylation facilitates SR Ca2+ release in the basal state and during repeated contractions, providing a positive feedback between [Ca2+ ]i and SR Ca2+ release. 相似文献
5.
The non-linear and spatially inhomogeneous interactions of dendritic membrane potential signals that represent the first step in the induction of activity-dependent long-term synaptic plasticity are not fully understood, particularly in dendritic regions which are beyond the reach of electrode measurements. We combined voltage-sensitive-dye recordings and Ca2+ imaging of hippocampal CA1 pyramidal neurons to study large regions of the dendritic arbor, including branches of small diameter (distal apical and oblique dendrites). Dendritic membrane potential transients were monitored at high spatial resolution and correlated with supra-linear [Ca2+ ]i changes during one cycle of a repetitive patterned stimulation protocol that typically results in the induction of long-term potentiation (LTP). While the increase in the peak membrane depolarization during coincident pre- and post-synaptic activity was required for the induction of supra-linear [Ca2+ ]i signals shown to be necessary for LTP, the change in the baseline-to-peak amplitude of the backpropagating dendritic action potential (bAP) was not critical in this process. At different dendritic locations, the baseline-to-peak amplitude of the bAP could be either increased, decreased or unaltered at sites where EPSP–AP pairing evoked supra-linear summation of [Ca2+ ]i transients. We suggest that modulations in the bAP baseline-to-peak amplitude by local EPSPs act as a mechanism that brings the membrane potential into the optimal range for Ca2+ influx through NMDA receptors (0 to −15 mV); this may require either boosting or the reduction of the bAP, depending on the initial size of both signals. 相似文献
6.
Streptomycin and intracellular calcium modulate the response of single guinea-pig ventricular myocytes to axial stretch 总被引:7,自引:0,他引:7
We tested the hypothesis that both stretch-activated channels (SACs) and intracellular calcium ([Ca2+ ]i ) are important in the electrical response of single guinea-pig ventricular myocytes to axial stretch. Myocytes were attached to carbon fibre transducers and stretched, sarcomere length increased by approximately 9 %, and there was a prolongation of the action potential duration. Streptomycin, a blocker of SACs, had no effect upon the shortening, [Ca2+ ]i transients or action potentials of electrically stimulated, unstretched myocytes, at a concentration of 50 μ m , but at 40 μ m , prevented any stretch-induced increase in action potential duration. Under action potential clamp, stretch elicited a current with a linear current-voltage relationship that was inward at membrane potentials negative to its reversal potential of −30 mV, in 10 of 24 cells tested, and was consistent with the activation of non-specific, cationic SACs. This current was not seen in any stretched cells that were exposed to 40 μ m streptomycin. However, exposure of cells to 5 μ m BAPTA-AM, in order to reduce [Ca2+ ]i transients, also abolished stretch-induced prolongation of the action potential. We conclude that both SACs and [Ca2+ ]i are important in the electrical response of cardiac myocytes to stretch, and propose that stretch-induced changes in electrical activity and [Ca2+ ]i may be linked by inter-dependent mechanisms. 相似文献
7.
Waves of calcium-induced calcium release occur in a variety of cell types and have been implicated in the origin of cardiac arrhythmias. We have investigated the effects of inhibiting the SR Ca2+ -ATPase (SERCA) with the reversible inhibitor 2',5'-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on the properties of these waves. Cardiac myocytes were voltage clamped at a constant potential between −65 and −40 mV and spontaneous waves evoked by increasing external Ca2+ concentration to 4 m m . Application of 100 μ m TBQ decreased the frequency of waves. This was associated with increases of resting [Ca2+ ]i , the time constant of decay of [Ca2+ ]i and the integral of the accompanying Na+ –Ca2+ exchange current. There was also a decrease in propagation velocity of the waves. There was an increase of the calculated Ca2+ efflux per wave. The SR Ca2+ content when a wave was about to propagate decreased to 91.7 ± 3.2%. The period between waves increased in direct proportion to the Ca2+ efflux per wave meaning that TBQ had no effect on the Ca2+ efflux per unit time. We conclude that (i) decreased wave frequency is not a direct consequence of decreased Ca2+ pumping by SERCA between waves but, rather, to more Ca2+ loss on each wave; (ii) inhibiting SERCA increases the chance of spontaneous Ca2+ release propagating at a given SR content. 相似文献
8.
Keith W. Dilly Charles F. Rossow V. Scott Votaw James S. Meabon Jennifer L. Cabarrus Luis F. Santana 《The Journal of physiology》2006,572(1):227-241
Ca2+ release during excitation–contraction (EC) coupling varies across the left ventricular free wall. Here, we investigated the mechanisms underlying EC coupling differences between mouse left ventricular epicardial (Epi) and endocardial (Endo) myocytes. We found that diastolic and systolic [Ca2+ ]i was higher in paced Endo than in Epi myocytes. Our data indicated that differences in action potential (AP) waveform between Epi and Endo cells only partially accounted for differences in [Ca2+ ]i . Rather, we found that the amplitude of the [Ca2+ ]i transient, but not its trigger – the Ca2+ current – was larger in Endo than in Epi cells. We also found that spontaneous Ca2+ spark activity was about 2.8-fold higher in Endo than in Epi cells. Interestingly, ryanodine receptor type 2 (RyR2) protein expression was nearly 2-fold higher in Endo than in Epi myocytes. Finally, we observed less Na+ –Ca2+ exchanger function in Endo than in Epi cells, which was associated with decreased Ca2+ efflux during the AP; this contributed to higher diastolic [Ca2+ ]i and SR Ca2+ in Endo than in Epi cells during pacing. We propose that transmural differences in AP waveform, SR Ca2+ release, and Na+ –Ca2+ exchanger function underlie differences in [Ca2+ ]i and EC coupling across the left ventricular free wall. 相似文献
9.
Clathrin-mediated endocytosis in snake motor terminals is directly facilitated by intracellular Ca2+
At the snake neuromuscular junction, low temperature (LT, 5–7°C) blocks clathrin-mediated endocytosis (CME) while exocytosis is largely unaffected. Thus compensatory endocytosis that normally follows transmitter release is inhibited, or 'delayed' until the preparation is warmed to room temperature (RT). This delay was exploited to observe how changes in bulk [Ca2+ ]i directly affect CME. Motor terminals were loaded with fura-2 to monitor [Ca2+ ]i . With brief stimulation at LT, [Ca2+ ]i transiently increased but returned to baseline (∼63 n m ) in < 8 min. After 15 min at LT, [Ca2+ ]i was altered by incubating preparations in the Ca2+ ionophore ionomyocin. Preparations were then warmed to RT to initiate delayed endocytosis, which was quantified as uptake of the fluorescent optical probe sulforhodamine 101. Endocytosis was more rapid when [Ca2+ ]i increased; the rate at 300 n m Ca2+ was ∼double that under basal conditions. Thus the rate of CME – isolated from stimulation, transmitter release, and other forms of endocytosis – is directly influenced by intraterminal Ca2+ . 相似文献
10.
11.
Capacitative calcium entry supports calcium oscillations in human embryonic kidney cells 总被引:4,自引:4,他引:4
Treatment of human epithelial kidney (HEK293) cells with low concentrations of the muscarinic agonist methacholine results in the activation of complex and repetitive cycling of intracellular calcium ([Ca2+ ]i ), known as [Ca2+ ]i oscillations. These oscillations occur with a frequency that depends on the concentration of methacholine, whereas the magnitude of the [Ca2+ ]i spikes does not. The oscillations do not persist in the absence of extracellular Ca2+ , leading to the conclusion that entry of Ca2+ across the plasma membrane plays a significant role in either their initiation or maintenance. However, treatment of cells with high concentrations of GdCl3 , a condition which limits the flux of calcium ions across the plasma membrane in both directions, allows sustained [Ca2+ ]i oscillations to occur. This suggests that the mechanisms that both initiate and regenerate [Ca2+ ]i oscillations are intrinsic to the intracellular milieu and do not require entry of extracellular Ca2+ . This would additionally suggest that, under normal conditions, the role of calcium entry is to sustain [Ca2+ ]i oscillations. By utilizing relatively specific pharmacological manoeuvres we provide evidence that the Ca2+ entry that supports Ca2+ oscillations occurs through the store-operated or capacitative calcium entry pathway. However, by artificial introduction of a non-store-operated pathway into the cells (TRPC3 channels), we find that other Ca2+ entry mechanisms can influence oscillation frequency in addition to the store-operated channels. 相似文献
12.
Dissociation of the store-operated calcium current ICRAC and the Mg-nucleotide-regulated metal ion current MagNuM 总被引:5,自引:5,他引:0
Meredith C. Hermosura Mahealani K. Monteilh-Zoller rew M. Scharenberg Reinhold Penner rea Fleig 《The Journal of physiology》2002,539(2):445-458
Rat basophilic leukaemia cells (RBL-2H3-M1) were used to study the characteristics of the store-operated Ca2+ release-activated Ca2+ current ( I CRAC ) and the magnesium-nucleotide-regulated metal cation current (MagNuM) (which is conducted by the LTRPC7 channel). Pipette solutions containing 10 m m BAPTA and no added ATP induced both currents in the same cell, but the time to half-maximal activation for MagNuM was about two to three times slower than that of I CRAC . Differential suppression of I CRAC was achieved by buffering free [Ca2+ ]i to 90 n m and selective inhibition of MagNuM was accomplished by intracellular solutions containing 6 m m Mg.ATP, 1.2 m m free [Mg2+ ]i or 100 μ m GTP-γ-S, allowing investigations on these currents in relative isolation. Removal of extracellular Ca2+ and Mg2+ caused both currents to be carried significantly by monovalent ions. In the absence or presence of free [Mg2+ ]i , I CRAC carried by monovalent ions inactivated more rapidly and more completely than MagNuM carried by monovalent ions. Since several studies have used divalent-free solutions on either side of the membrane to study selectivity and single-channel behaviour of I CRAC , these experimental conditions would have favoured the contribution of MagNuM to monovalent conductance and call for caution in interpreting results where both I CRAC and MagNuM are activated. 相似文献
13.
Electrical rhythmicity in smooth muscle cells is essential for the movement of the gastrointestinal tract. Interstitial cells of Cajal (ICC) lie adjacent to smooth muscle layers and are implicated as the pacemaker cells. However, the pace making mechanism remains unclear. To study the intercellular interaction during electrical rhythm generation, we visualized changes in intracellular Ca2+ concentration ([Ca2+ ]i ) in smooth muscle cells and myenteric ICC within segments of mouse ileum loaded with a fluorescent Ca2+ indicator, fluo-3. We observed rhythmic [Ca2+ ]i changes in longitudinal smooth muscle cells travelling rapidly through the smooth muscle cell layer. Between the rhythmic Ca2+ transients, we found brief Ca2+ transients localized to small areas within smooth muscle cells. The amplitude but not the periodicity of rhythmic [Ca2+ ]i transients in both cell types was partially inhibited by nicardipine, an L-type Ca2+ channel antagonist, suggesting that the rhythmic [Ca2+ ]i transients reflect membrane potential depolarizations corresponding to both slow waves and triggered Ca2+ spikes. Longitudinal smooth muscle cells and myenteric ICC showed synchronous spontaneous [Ca2+ ]i transients in eight out of 21 ileac preparations analysed. In the remaining preparations, the synchrony between ICC and smooth muscle cells was absent, although the rhythmicity of the smooth muscle cells was not disturbed. These results suggest that myenteric ICC may play multiple roles including pace making for physiological bowel movement. 相似文献
14.
Barbara Wedel Rebecca R. Boyles James W. Putney Jr Gary S. Bird 《The Journal of physiology》2007,579(3):679-689
We have investigated the nature of the Ca2+ entry supporting [Ca2+ ]i oscillations in human embryonic kidney (HEK293) cells by examining the roles of recently described store-operated Ca2+ entry proteins, Stim1 and Orai1. Knockdown of Stim1 by RNA interference (RNAi) reduced the frequency of [Ca2+ ]i oscillations in response to a low concentration of methacholine to the level seen in the absence of external Ca2+ . However, knockdown of Stim1 did not block oscillations in canomical transient receptor potential 3 channel (TRPC3)-expressing cells and did not affect Ca2+ entry in response to arachidonic acid. The effects of knockdown of Stim1 could be reversed by inhibiting Ca2+ extrusion with a high concentration of Gd3+ , or by rescuing the knockdown by overexpression of Stim1. Similarly, knockdown of Orai1 abrogated [Ca2+ ]i oscillations, and this was reversed by use of high concentrations of Gd3+ ; however, knockdown of Orai1 did not affect arachidonic acid-activated entry. RNAi targeting 34 members of the transient receptor potential (TRP) channel superfamily did not reveal a role for any of these channel proteins in store-operated Ca2+ entry in HEK293 cells. These findings indicate that the Ca2+ entry supporting [Ca2+ ]i oscillations in HEK293 cells depends upon the Ca2+ sensor, Stim1, and calcium release-activated Ca2+ channel protein, Orai1, and provide further support for our conclusion that it is the store-operated mechanism that plays the major role in this pathway. 相似文献
15.
Juan Martinez-Pinna Gwen Tolhurst Iman S. Gurung Jamie I. Vandenberg Martyn P. Mahaut-Smith 《The Journal of physiology》2004,555(1):61-70
G-protein-coupled receptor signalling has been suggested to be voltage dependent in a number of cell types; however, the limits of sensitivity of this potentially important phenomenon are unknown. Using the non-excitable rat megakaryocyte as a model system, we now show that P2Y receptor-evoked Ca2+ mobilization is controlled by membrane voltage in a graded and bipolar manner without evidence for a discrete threshold potential. Throughout the range of potentials studied, the peak increase in intracellular Ca2+ concentration ([Ca2+ ]i ) in response to depolarization was always larger than the maximal reduction in [Ca2+ ]i following an equivalent amplitude hyperpolarization. Significant [Ca2+ ]i increases were observed in response to small amplitude (<5 mV, 5 s duration) or short duration (25 ms, 135 mV) depolarizations. Individual cardiac action potential waveforms were also able to repeatedly potentiate P2Y receptor-evoked Ca2+ release and the response to trains of normally paced stimuli fused to generate prolonged [Ca2+ ]i increases. Furthermore, elevation of the temperature to physiological levels (36°C) resulted in a more sustained depolarization-evoked Ca2+ increase compared with more transient or oscillatory responses at 20–24°C. The ability of signalling via a G-protein-coupled receptor to be potentiated by action potential waveforms and small amplitude depolarizations has broad implications in excitable and non-excitable tissues. 相似文献
16.
Discrete store-operated calcium influx into an intracellular compartment in rabbit arteriolar smooth muscle 总被引:1,自引:3,他引:1
This study tested the hypothesis that store-operated channels (SOCs) exist as a discrete population of Ca2+ channels activated by depletion of intracellular Ca2+ stores in cerebral arteriolar smooth muscle cells and explored their direct contractile function. Using the Ca2+ indicator fura-PE3 it was observed that depletion of sarcoplasmic reticulum (SR) Ca2+ by inhibition of SR Ca2+ -ATPase (SERCA) led to sustained elevation of [Ca2+ ]i that depended on extracellular Ca2+ and slightly enhanced Mn2+ entry. Enhanced background Ca2+ influx did not explain the raised [Ca2+ ]i in response to SERCA inhibitors because it had marked gadolinium (Gd3+ ) sensitivity, which background pathways did not. Effects were not secondary to changes in membrane potential. Thus SR Ca2+ depletion activated SOCs. Strikingly, SOC-mediated Ca2+ influx did not evoke constriction of the arterioles, which were in a resting state. This was despite the fura-PE3-indicated [Ca2+ ]i rise being greater than that evoked by 20 m m [K+ ]o (which did cause constriction). Release of endothelial vasodilators did not explain the absence of SOC-mediated constriction, nor did a change in Ca2+ sensitivity of the contractile proteins. We suggest SOCs are a discrete subset of Ca2+ channels allowing Ca2+ influx into a 'non-contractile' compartment in cerebral arteriolar smooth muscle cells. 相似文献
17.
Spatial Segregation and Interaction of Calcium Signalling Mechanisms in Rat Hippocampal CA1 Pyramidal Neurons 总被引:4,自引:5,他引:4
Takeshi Nakamura Nechama Lasser-Ross Kyoko Nakamura William N. Ross 《The Journal of physiology》2002,543(2):465-480
Postsynaptic [Ca2+ ]i increases result from Ca2+ entry through ligand-gated channels, entry through voltage-gated channels, or release from intracellular stores. We found that these sources have distinct spatial distributions in hippocampal CA1 pyramidal neurons. Large amplitude regenerative release of Ca2+ from IP3 -sensitive stores in the form of Ca2+ waves were found almost exclusively on the thick apical shaft. Smaller release events did not extend more than 15 μm into the oblique dendrites. These synaptically activated regenerative waves initiated at points where the stimulated oblique dendrites branch from the apical shaft. In contrast, NMDA receptor-mediated increases were observed predominantly in oblique dendrites where spines are found at high density. These [Ca2+ ]i increases were typically more than eight times larger than [Ca2+ ]i from this source on the main aspiny apical shaft. Ca2+ entry through voltage-gated channels, activated by backpropagating action potentials, was detected at all dendritic locations. These mechanisms were not independent. Ca2+ entry through NMDA receptor channels or voltage-gated channels (as previously demonstrated) synergistically enhanced Ca2+ release generated by mGluR mobilization of IP3 . 相似文献
18.
Charlotta S. Olofsson Albert Salehi Cecilia Holm Patrik Rorsman 《The Journal of physiology》2004,557(3):935-948
We have investigated the in vitro effects of the saturated free fatty acid palmitate on mouse pancreatic β-cells by a combination of electrophysiological recordings, intracellular Ca2+ ([Ca2+ ]i ) microfluorimetry and insulin release measurements. Addition of palmitate (1 m m , bound to fatty acid-free albumin) to intact islets exposed to 15 m m glucose increased the [Ca2+ ]i by ∼30% and insulin secretion 2-fold. Palmitate remained capable of increasing [Ca2+ ]i and insulin release in the presence of tolbutamide and in islets depolarized by high K+ in combination with diazoxide, indicating that the stimulation occurs independently of closure of ATP-regulated K+ channels (KATP channels). Palmitate (0.5 m m ) augmented exocytosis (measured as an increase in cell capacitance) in single β-cells and increased the size of the readily releasable pool (RRP) of granules 2-fold. Whole-cell peak Ca2+ currents rose by ∼25% following addition of 0.5 m m palmitate, an effect that was abolished in the presence of 10 μ m isradipine indicating that the free fatty acid specifically acts on L-type Ca2+ channels. The actions of palmitate on exocytosis and Ca2+ currents were not mimicked by intracellular application of palmitoyl-CoA. We conclude that palmitate increases insulin secretion by a KATP channel-independent mechanism exerted at the level of exocytosis and that involves both augmentation of L-type Ca2+ currents and an increased size of the RRP. 相似文献
19.
Intense motor neuron activity induces a long-term facilitation (LTF) of synaptic transmission at crayfish neuromuscular junctions (NMJs) that is accompanied by an increase in the accumulation of presynaptic Ca2+ ions during a test train of action potentials. It is natural to assume that the increased Ca2+ influx during action potentials is directly responsible for the increased transmitter release in LTF, especially as the magnitudes of LTF and increased Ca2+ influx are positively correlated. However, our results indicate that the elevated Ca2+ entry occurs through the reverse mode operation of presynaptic Na+ /Ca2+ exchangers that are activated by an LTF-inducing tetanus. Inhibition of Na+ /Ca2+ exchange blocks this additional Ca2+ influx without affecting LTF, showing that LTF is not a consequence of the regulation of these transporters and is not directly related to the increase in [Ca2+ ]i reached during a train of action potentials. Their correlation is probably due to both being induced independently by the strong [Ca2+ ]i elevation accompanying LTF-inducing stimuli. Our results reveal a new form of regulation of neuronal Na+ /Ca2+ exchange that does not directly alter the strength of synaptic transmission. 相似文献
20.
Romain Guinamard Aurélien Chatelier Marie Demion Daniel Potreau Sylvie Patri Mohammad Rahmati Patrick Bois 《The Journal of physiology》2004,558(1):75-83
Cardiac arrhythmias, which occur in a wide variety of conditions where intracellular calcium is increased, have been attributed to the activation of a transient inward current ( I ti ). I ti is the result of three different [Ca]i -sensitive currents: the Na+ –Ca2+ exchange current, a Ca2+ -activated chloride current and a Ca2+ -activated non-selective cationic current. Using the cell-free configuration of the patch-clamp technique, we have characterized the properties of a Ca2+ -activated non-selective cation channel (NSCCa ) in freshly dissociated human atrial cardiomyocytes. In excised inside-out patches, the channel presented a linear I–V relationship with a conductance of 19 ± 0.4 pS. It discriminated poorly among monovalent cations (Na+ and K+ ) and was slightly permeable to Ca2+ ions. The channel's open probability was increased by depolarization and a rise in internal calcium, for which the K d for [Ca2+ ]i was 20.8 μ m . Channel activity was reduced in the presence of 0.5 m m ATP or 10 μ m glibenclamide on the cytoplasmic side to 22.1 ± 16.8 and 28.5 ± 8.6%, respectively, of control. It was also inhibited by 0.1 m m flufenamic acid. The channel shares several properties with TRPM4b and TRPM5, two members of the 'TRP melastatin' subfamily. In conclusion, the NSCCa channel is a serious candidate to support the delayed after-depolarizations observed in [Ca2+ ] overload and thus may be implicated in the genesis of arrhythmias. 相似文献