哮喘儿童呼吸道合胞病毒感染后血清抗F和抗G蛋白特异性IgG抗体？…

目的 探讨血清抗呼吸道合胞病毒（ＲＳＶ）抗Ｆ和抗Ｇ蛋白ＩｇＧ抗体水平与哮喘儿童ＲＳＶ感染及感染后病情轻重的关系。方法 以ＲＳＶ（Ｌｏｎｇ株）及表达ＲＳＶ Ｆ和Ｇ蛋白的重组痘苗病毒为抗原，采用酶联免疫吸附试验，检测了６６例６岁以下哮喘患儿ＲＳＶ流行期间双份血清中抗ＲＳＶ Ｆ和Ｇ蛋白特异性ＩｇＧ抗体水平。结果 （１）本组哮喘患儿ＲＳＶ感染率为３５％；（２）ＲＳＶ感染组哮喘患儿血清抗Ｆ和Ｇ蛋白ＩｇＧ抗体     

哮喘儿童呼吸道合胞病毒感染后血清抗F和抗G蛋白特异性IgG抗体的变化

目的探讨血清抗呼吸道合胞病毒（ＲＳＶ）抗Ｆ和抗Ｇ蛋白ＩｇＧ抗体水平与哮喘儿童ＲＳＶ感染及感染后病情轻重的关系。方法以ＲＳＶ（Ｌｏｎｇ株）及表达ＲＳＶＦ和Ｇ蛋白的重组痘苗病毒为抗原，采用酶联免疫吸附试验，检测了６６例６岁以下哮喘患儿ＲＳＶ流行期间双份血清中抗ＲＳＶＦ和Ｇ蛋白特异性ＩｇＧ抗体水平。结果（１）本组哮喘患儿ＲＳＶ感染率为３５％；（２）ＲＳＶ感染组哮喘患儿血清抗Ｆ和Ｇ蛋白ＩｇＧ抗体滴度明显低于非ＲＳＶ感染组；（３）ＲＳＶ感染后哮喘中～重度发作组血清抗Ｆ蛋白ＩｇＧ抗体滴度明显低于轻度发作组；（４）哮喘患儿ＲＳＶ感染后恢复期较急性期血清抗Ｆ和Ｇ蛋白抗体滴度均有明显升高，其升高程度在不同年龄组及两种蛋白间差异均无显著意义。结论血清抗Ｆ和抗Ｇ蛋白特异性ＩｇＧ抗体对哮喘患儿ＲＳＶ感染有一定保护作用；ＲＳＶ感染后抗Ｆ和抗Ｇ蛋白特异性ＩｇＧ抗体滴度的高低亦反映哮喘急性发作的轻重程度     

呼吸道合胞病毒疫苗的研究和临床应用

简介呼吸道合胞病毒（ＲＳＶ）的生物学特点和疫苗研究的进展。已经和正在研制的 ＲＳＶ疫苗包括：（１）福尔马林灭活ＲＳＶ疫苗；（２）活病毒疫苗，是通过冷传代和温度敏感传代获得的ＲＳＶ减毒活疫苗，其免疫反应与自然感染相似，感染野生型ＲＳＶ不加剧病情，能被动获得ＲＳＶ中和抗体产生保护性免疫反应，是目前正在婴幼儿中试用观察的疫苗；（３）ｃＤＮＡ疫苗，是从ＲＳＶ ｃＤＮＡ克隆中回收具有感染性重组ＲＳＶ的活病毒疫苗，在下呼吸道高度减活，可显著提高对野生型－ＲＳＶ感染的免疫力，安全性好，可构建含有ＲＳＶ Ａ、Ｂ组的 Ｇ基因ｃＤＮＡ２价疫苗；（４）载体疫苗，ＲＳＶ Ｆ、Ｇ嵌合疫苗由表达含有ＲＳＶＦ和Ｇ蛋白的杆状病毒纯化获得；（５）亚单位疫苗，对下呼吸道感染具有保护作用，中和抗体滴度高；（６）合成肽类疫苗等。     

呼吸道合胞病毒免疫球蛋白治疗儿童骨髓移植后下呼吸道感染

呼吸道合胞病毒（ＲＳＶ）感染是骨髓移植（ＢＭＴ）后常见的并发症之一．据报道ＢＭＴ患者并发ＲＳＶ下呼吸道感染,若未经抗病毒治疗病死率接近１００％,而应用利巴韦林（ｒｉｂａｒｉｒｉｎ）治疗者病死率高达５０％－７０％．报道静注呼吸道合胞病毒免疫球蛋白（ＲＳＶ－ＩＧＹＶ）治疗儿童ＢＭＴ合并ＲＳＶ感染的疗效． 病例和方法ＲＳＶ－ＩＧＩＶ是一种含有高浓度ＲＳＶ中和抗体（抗体滴度＞１９２００ＭＵ／ｍｌ）的ＩｇＧ免疫球蛋白．１９９１年６月至１９９６年２月,１１例ＢＭＴ合并ＲＳＶ感染患儿接受了ＲＳＶ－ＩＧＩＶ治疗…     

应用免疫酶组化法检测外周血呼吸道合胞病毒IgM抗体的研究

应用免疫酶组化法检测外周血呼吸道合胞病毒ＩｇＭ抗体的研究张传湘胡姣荣刘孟秋刘仲熊呼吸道合胞病毒（ＲＳＶ）是婴幼儿急性下呼吸道感染（ＡＬＲＩ）的重要病原，可致喘憋性肺炎流行和院内交叉感染，探索简便、快速、敏感、特异的诊断方法十分必要。我们应用ＲＳＶ抗原...     

小剂量α-干扰素降低呼吸道合胞病毒感染后哮喘患病率的研究

呼吸道合胞病毒 (ＲＳＶ)是引起婴幼儿严重下呼吸道感染的重要病原之一 ,并与儿童哮喘关系密切 ,不仅能诱发哮喘 ,且与哮喘的病情加重有关。为寻找降低ＲＳＶ感染后哮喘患病率的有效方法 ,我科从１９９４年至１９９８年对ＲＳＶ感染患儿应用小剂量α -干扰素治疗 ,疗效明显 ,现报道如下。资料与方法一、对象全部病例均为我院住院患儿 ,经鼻咽部分泌物免疫荧光ＲＳＶ检测或血清特异性ＲＳＶ＿ＩｇＭ阳性者。随机分为观察组和对照组 ,观察组６０例 ,男３６例 ,女２４例 ,年龄１月～２岁３月 ;对照组５５例 ,男２８例 ,女２７例 ,年龄１月～２…     

快速诊断单纯疱疹病毒脑炎

目的探讨快速诊断单纯疱疹病毒脑炎（ＨＳＥ），比较不同病毒学试验的诊断价值。方法用聚合酶链反应技术检测１７７例急性脑炎患儿的脑脊液（ＣＳＦ）标本中单纯疱疹病毒（ＨＳＶ）特异性ＤＮＡ；用酶联免疫吸附方法检测ＣＳＦ和血清标本中ＨＳＶ特异性ＩｇＭ和ＩｇＧ抗体。结果ＣＳＦ中ＨＳＶ特异性ＤＮＡ、ＩｇＭ和ＩｇＧ抗体阳性率分别为１．７％（３／１７７）、１０％（１／１００）和４７０％（４７／１００），血清ＨＳＶＩｇＭ、ＩｇＧ抗体阳性率分别为１２．５％（６／４８）、７２．９％（５１／７０）（因为标本量不足或缺如，未能对全部病例进行抗体检测）；３例患儿确诊为ＨＳＥ。结论用套式ＰＣＲ检测ＣＳＦ诊断ＨＳＥ较敏感、特异。     

细胞因子与呼吸道合胞病毒感染

呼吸道合胞病毒（ＲＳＶ）是引起婴幼儿毛细支气管炎和肺炎的最常见病原体，越来越多的研究表明ＲＳＶ感染的发病机制与机体异常的免疫病理有关。ＲＳＶ感染后细胞因子的变化，如：ＩＬ－１抑制物的产生，ＩＬ－２活性降低，ＩＬ－２Ｒ表达障碍，ＩＦＮγ减少和ＩＬ－４、ＩＬ－５、ＩＬ－６、ＩＬ－８、ＴＮＦα的活性增强，在ＲＳＶ感染的免疫保护和免疫病理中起重要作用。     

巨细胞病毒性肝炎特异性IgG亚群检测及临床意义

对２１例婴儿巨细胞病毒性肝炎患儿（简称肝炎组）与１０例无症状巨细胞病毒感染婴儿（简称无症状组），应用ＥＬＩＳＡ方法进行抗巨细胞病毒（ＣＭＶ）ＩｇＧ亚群检测，肝炎组中１３例做了恢复期的复查。结果表明，肝炎组抗ＣＭＶＩｇＧ＿２、ＩｇＧ＿４值均为较低水平，无症状组抗ＣＭＶＩｇＧ＿１、ＩｇＧ＿３，值明显高于肝炎组；肝炎组恢复期抗ＣＭＶＩｇＧ＿３明显增高，而抗ＣＭＶＩｇＧ＿１增高不显著。提示体液免疫反应特别是抗ＣＭＶＩｇＧ＿３在抗ＣＭＶ感染中起到一定作用，对ＣＭＶ感染高危婴幼儿可考虑应用含高滴度抗ＣＭＶ抗体的免疫球蛋白。     

输血后丙型肝炎抗C22,C33C和NS5IgG及其亚群的检测和意义

为探讨抗丙型肝炎病毒（ＨＣＶ）ＩｇＧ型抗体及其亚群在ＨＣＶ感染过程中的作用，采用酶联免疫吸附法对６６例输血后ＨＣＶ感染患儿的９２份急、慢性期血清和３２份α干扰素（αＩＦＮ）治疗后血清进行抗Ｃ２２、抗Ｃ３３Ｃ和抗ＮＳ５及其亚群的检测。结果表明，急性期抗Ｃ２２和抗Ｃ３３Ｃ检出率高，分别为９８．４３％和１００．００％；而抗ＮＳ５在急性期检出率仅为５３．１２％，明显低于慢性期（９１．６７％）和同期抗Ｃ２２和抗Ｃ３３Ｃ检出率（分别为９６．８８％和１００．００％）。三种抗体ＩｇＧ亚群在急性期的检出率和抗体水平均显著低于慢性期。抗Ｃ２２ＩｇＧ１为该抗体的优势亚群；而抗Ｃ３３Ｃ无明显优势亚群；抗ＮＳ５ＩｇＧ１及ＩｇＧ３至慢性期才显示其优势。６６例患儿中，１３例接受αＩＦＮ治疗，其中５例复发患儿在ＨＣＶＲＮＡ再次出现时，抗Ｃ２２ＩｇＧ１呈现有意义的下降，而抗Ｃ２２总水平并无明显变化，而接受αＩＦＮ治疗４例有效和另４例无效患儿无类似变化。提示抗Ｃ２２和抗Ｃ３３Ｃ是诊断急性ＨＣＶ感染的敏感指标；抗Ｃ２２ＩｇＧ１可能有助于评价αＩＦＮ治疗ＨＣＶ感染的疗效。     

Book Reviews

HANDBOOK OF PEDIATRICS, by Henry K. Silver, C. Henry Kempe, Henry B. Bruyn, Vincent A. Fulginiti
ESSENTIAL MEDICAL GENETICS, 2nd edn, by J. M. Connor and M.A. Ferguson-Smith
SEVERE BEHAVIOR DISORDERS OF CHILDREN AND YOUTH, by Robert B. Rutherford, Jr, C. Michael Nelson, Steven R. Forness
PATIENT CARE IN PEDIATRIC SURGERY, by L. L. Leape
PRIMARY CARE OF THE NEWBORN, by R. W. Coen, H. Koffler
PROGRESS IN RESPIRATION RESEARCH SERIES, edited by H. Herzog: VOLUME 22: LUNG FUNCTION IN CHILDREN AND ADOLESCENTS, by A. Zapletal, M. Samanek, T. Paul
UPPER-LIMB DEFICIENCIES IN CHILDREN: PROSTHETIC, ORTHOTIC, AND SURGICAL MANAGEMENT, by D. W. Lamb, H. T. Law
POCKET GUIDE TO PEDIATRIC FRACTURES, by J. A. Ogden
CLINICAL EXAMINATION OF THE INJURED KNEE, M. Cross, K. Crichton
DIAGNOSTIC RECOGNITION OF GENETIC DISEASE, by W. Nyhan, N. Sakati     

Book Review

Organ Transplantation in Children (Perspectives in Pediatric Pathology, Volume 13) Edited by C . R. Abramowsky and R. B. Colvin Basel, Karper, 1989, 205 pages, $1 52.00.

Basel Louis P. Dehner, MD, Karger, 1989, 205 pages, $152.00. Edited by Colin L. Berry London, Springer- Wag, 1989, 830 pages, $200.00.

Metabolic Bone Disease in Children (Clinical Pediatric Series no. 6) Edited by S. Castells and L. Finberg New Krk, Marcel Dekker, 1990, 408 tagel, $99.75.

New York Peter Revell, Marcel Dekker,1990, 408 pages, $99.75. Edited by S. Castells and L. Finberg New Krk, Marcel Dekker, 1990, 408 tagel, $99.75.     

Book Reviews

ACNE AND ROSACEA, 2nd edition, by G. Plewig and A. M. Kligman
PAEDIATRIC GASTROENTEROLOGY, edited by J. Navarro and J. Schmitz
BREAKING OUT, edited by R. Kosky, H. S. Eshkevari and G. Kneebone.
LEONARDO DA VINCI THE ANATOMY OF MAN, by M. Clayton
EFFECTIVE CARE OF THE NEWBORN INFANT, edited by J. C. Sinclair and M. B. Bracken
PRINCIPLES AND PRACTICE OF DERMATOLOGY, 3rd edition, by H. Rotstein
CHILDREN AND COUNSELLING, by M. Crompton
PAEDIATRIC HANDBOOK, 4th edition, edited by staff, Royal Children's Hospital, Melbourne
MUSCLES, MASSES & MOTION. THE PHYSIOLOGY OF NORMALITY, HYPOTONICITY, SPASTICITY & RIGIDITY, by E. G. Walsh
HEALTH FOR ALL CHILDREN, 2nd edition, edited by D. M. B. Hall     

BOOK REVIEW

Kozlowski PB, Snider DA, Vietze PM, Wisniewski HM, eds. Brain in Pediatric AIDS (Proceedings of the Conference on Brain in Pediatr HIV Infection, New York, July 24–25, 1989). Basel: Karger, 1990.
Philip A. Mackowiak, ed, Fever–Basic Mechanisms and Management. New York: Raven Press, 1991.
Pediatric Allergy and Immunology. Copenhagen: Munksgaard. Four issues per year.
Siegel M, ed. Pediatric Ultrasonography. New York: Raven Press, 1990.
Amir N, Rapin I, Branski D, eds. Pediatric Neurology: Behaviour and Cognition of the Child with Brain Dysfunction. In: Pediatric and Adolescent Medicine, Vol. 1. Basel: S. Karger AG, 1991.
Gorlin RJ, Cohen MM, Levin LS, eds. Syndromes of the Head and Neck. 3rd ed. Oxford Monographs on Medical Genetics No. 19. Oxford: Oxford University Press 1990.     

Book Reviews

Book reviewed in this article:
SEA STINGERS, by L. Marsh and S. Slack-Smith. Perth: Western Australian Museum
COMMUNITY CHILD HEALTH: AN AUSTRALIAN PERSPECTIVE, edited by G. Vimpani and T. Parry
LOSS OF A BABY: UNDERSTANDING MATERNAL GRIEF, by M. Nicol
AN ANNOTATED BIBLIOGRAPHY: ABORIGINAL HEALTH, by N. Thomson and P. Merrifield
LIFE AFTER LOSS: GETTING OVER GRIEF, GETTING ON WITH LIFE, by Francis MacNab
COLOR ATLAS OF CHILD SEXUAL ABUSE, by D. L. Chadwick, C. D. Berkowitz, D. Kerns
YEAR BOOK OF PEDIATRICS 1989, edited by F. A. Oski and J. A. Stockman III
ADVANCES IN PEDIATRICS, VOL. 35, edited by L. A. Barness, D. C. DeVivo, G. Morow III, F. Oski and A. M. Rudolph
CURRENT PROBLEMS IN PAEDIATRICS, edited by J. D. Lockhart
HANDBOOK OF HAEMATOLOGICAL INVESTIGATIONS IN CHILDREN, by R. F. Stevens
FAMILY MEDICINE: PRINCIPLES AND PRACTICE, 3rd edn, edited by J. L. Buckingham, E. P. Donatelle, T. A. Johnson Jr and J. E. Scherger
CURRENT PROBLEMS IN SURGERY, by M. M. Ravitch and F. M. Steichen
CLINICAL NUTRITION IN PAEDIATRIC DISORDERS, by D. Bentley and M. Lawson
CORE TEXTBOOK OF PEDIATRICS, 3rd edn, by R. Kay, F. A. Oski and L. A. Barness
HANDBOOK OF RENAL INVESTIGATIONS IN CHILDREN, by C. M. Taylor and S. Chapman
PAEDIATRIC NEUROLOGY—PRINCIPLES AND PRACTICE, by K. F. Swaiman     

Stellungnahme zur Verwendung von Säuglingsnahrungen auf Sojaeiweißbasis

Ohne ZusammenfassungErnährungskommission der Deutschen Gesellschaft für Kinder- und Jugendmedizin: H. J. Böhles, C. Fusch, O. Genzel-Boroviczeny, J. Henker, B. Koletzko (Vorsitzender), M. Kersting (Gast), M. Lentze, R. G. Maaser, F. Manz, W. Mihatsch, F. Pohlandt, H. Przyrembel (Gast)Ernährungskommission der Schweizerischen Gesellschaft für Pädiatrie: C. P. Braegger (Präsident), P. Baehler, O. Baenziger, D. Belli, G. Deleze, R. Furlano, J. Laimbacher, M. Roulet, J. Spalinger, P. Studer     

脐血造血调节因子活性的研究

探讨脐血中造血调节因子含量、来源及其与造血细胞间的关系。方法：双抗夹心酶联免疫吸附（ELISA）法和细胞因子依赖细胞株增殖反应MTT法测定脐血和脐血细胞培养上清中颗粒细胞集落刺激因子（G－CSF）、颗粒-巨噬细胞集落刺激因子（GM－CSF）、白细胞介素-6（IL－6）和肿瘤坏死因子-α（TNFα），并观察脐血造血调节因子与脐血CFU-GM、MNC、WBC相关关系。结果：正向造血调节因子G-CSF、GM－CSF和IL－6的含量明显高于成人外周血，负向调节因子TNFα测不出；脐血G－CSF、GM－CSF分别和WBC、CFU-GM成正相关，IL-6与CFU-GM成负相关，脐血、外周血和骨髓单个核细胞（MNC）培养上清液中G－CSF、IL－6产量相近,而脐血MNC培养上清中GM－CSF产量低于外周血和骨髓。结论：脐血浆有较高正向造血刺激活性，维持着胎儿高水平造血；脐血细胞自身不产生大量细胞因子。胎盘很可能是脐血细胞因子的重要来源之一。     

Book Reviews

Book reviewed in this article:
RECOGNIZING PATTERNS OF OCULAR CHILDHOOD DISEASES, by Leonard B. Nelson, Gary C. Brown, Juan J. Arentsen
THE CHROMOSOMES AND THEIR DISORDERS: AN INTRODUCTION FOR CLINICIANS, 4th edn, by G. H. Valentine
RELATED SERVICES FOR HANDICAPPED CHILDREN, by Morton M. Esterson and Linda F. Bluth
PERSPECTIVES IN PAEDIATRIC PATHOLOGY, Series Editors: Harvey S. Rosenberg and Jay Bernstein, Volume 9: NEOPLASIA IN INFANCY AND CHILDHOOD, edited by Harvey S. Rosenberg, Jay Bernstein and William A. A. Newton Jr
PEDIATRIC NEPHROLOGY, 2nd edn, edited by M. Holliday, T. Barratt and R. Vernier
CANCER IN CHILDREN: CLINICAL MANAGEMENT, 2nd edn. edited by P. A. Voute, A. Barrett, H. J. G. Bloom, J. Lemerle and M. K. Neidhardt     

BOOK REVIEWS

ABSTRACT. Eveleth PB, Tanner JM. Worldwide Variation in Human Growth , 2nd ed. Cambridge: Cambridge University Press, 1990.
Barkovich JA, Truwit LC. Practical MRI Atlas of Neonatal Brain Development. New York: Raven Press, 1990.
Ichikawa I, ed. Pediatric Textbook of Fluids and Electrolytes. Baltimore: Williams & Wilkins, 1990.
Advances in Pediatrics, vol 37. Barness LA, ed., De Vivo DC, Morrow III G, Oski F, Rudolph AM, assoc. eds. Chicago: Mosby Year Book, 1990.     

Book Reviews

Book reviewed in this article:
BEYOND SEPARATION. Further Studies of Children in Hospital, edited by David Hall and Margaret Stacey
MANUAL OF PEDIATRIC ANESTHESIA, by David J. Steward
PEDIATRIC NEUROMUSCULAR DISEASES, by Kenneth F. Swaiman and Francis S. Wright
CHILDREN WITH CANCER. A HANDBOOK FOR FAMILIES AND HELPERS, by Merren Parker and David Mauger
A COLOUR ATLAS OF ENDOCRINOLOGY, by Reginald Hall, David Evered and Raymond Greene
INBORN ERRORS OF IMMUNITY AND PHAGOCYTOSIS, edited by F. Guttler, J. W. T. Seakins, and R. A. Harkness
ADOLESCENT MEDICINE CASE STUDIES, by George D. Comerci, Elmer S. Lightner, and Ronald Hansen
PEDIATRIC BURNS, edited by Wm. Carl Bailey
A PRACTICAL GUIDE TO PAEDIATRIC INTENSIVE CARE, edited by Daniel L. Levin, Frances C. Morriss, and Gerald C. Moore
BABY SURGERY: NURSING MANAGEMENT AND CARE. 2nd edition, by Daniel G. Young and Eleanor J. Martin
PREGNANCY METABOLISM, DIABETES AND THE FETUS.