腹腔镜下腹股沟疝修补术在老年腹股沟复发疝中的应用

目的 评价腹腔镜腹股沟疝修补术在老年腹股沟复发疝应用的安全性和有效性.方法 回顾性分析2010年5月至2012年5月应用腹腔镜腹股沟疝修补术对腹股沟复发疝行手术治疗18例患者的临床资料.结果 18例患者手术时间52～ 138 min,平均(68±21.2) min;术后1d下床活动;住院时间4～7d,平均(5.2±1.36)d;术后无严重并发症发生.结论 腹腔镜腹股沟疝修补术在老年性复发疝中具有创伤小、术后恢复时间快、住院时间短及术后并发症少的优点.     

疝环充填式无张力疝修补术治疗腹股沟疝128例临床分析

目的：探讨疝环充填式无张力疝修补术的临床疗效。方法：回顾分析腹股沟疝行充填式无张力疝修补术128例的临床资料。结果：手术时间25—70min,平均38min,术后切口疼痛轻,恢复快,住院时间4～9d,平均5．5d。随访3月～4年6个月,无1例复发。结论：疝环充填式无张力疝修补术治疗腹股沟疝损伤小、痛苦轻、复发率低,应成为腹股沟疝修补术的首选手术方式。     

完全腹膜外腹腔镜疝修补术治疗腹股沟疝（附338例次报告）

目的 总结我科腹腔镜完全腹膜外腹股沟疝修补术338例次的经验教训.方法 292例病人共行338例次腹腔镜完全腹膜外腹股沟疝修补术,直疝57例次,斜疝281例次,单侧疝246例,双侧疝46例,其中复发疝42例.结果 中转经腹腔腹膜前修补6例次,完全腹膜外手术成功率98.23%.手术时间25～220 min,平均手术时间单侧60 min,双侧96 min.住院时间4～16 d,平均7 d.主要并发症为阴囊血清肿或血肿29例(9.93%),术后腹股沟区慢性疼痛4例(1.37%),手术大出血1例,膀胱损伤1例.无手术死亡.随访6个月～5年,术后复发1例.结论 腹腔镜完全腹膜外腹股沟疝修补术手术安全,复发率低.掌握手术技巧可提高手术成功率、降低术后并发症和复发率.     

腹腔镜全腹膜外腹股沟疝修补术80例

目的探讨腹腔镜全腹膜外腹股沟疝修补术（TEP）治疗腹股沟疝的方法及疗效。方法我院收治腹股沟疝80例,其中单侧疝65例,双侧疝15例,复发疝11例。行105次TEP手术,其中直疝24例次,斜疝81例。结果80例TEP均获成功,手术时间50-140min平均80min,术后住院5-8天平均6.5天。术后随访0.5-2年,平均12个月,均无复发。结论TEP是一种安全可靠的疝修补术,遵循无张力疝修补术原则,具有复发率低、创伤小等优点。     

腹股沟疝不同手术方式修补的比较

目的探讨腹股沟疝不同修补手术方法的优缺点.方法75例腹股沟疝行腹腔镜疝修补术,96例腹股沟疝行Bassini疝修补术,记录手术时间、术后下床活动时间、住院时间及各种并发症并进行比较.结果腹腔镜组手术全部成功完成,8例病人同时行阑尾切除术、4例病人同时行胆囊切除术.腹腔镜组手术时间、术后下床活动时间、住院时间较Bassini组少(P＜0.05).Bassini组术后并发症较腹腔镜组多,(χ 2=14.3339,P＜0.01).腹腔镜组1例(1.33%)复发,Bassini组4例(4.25%)复发,差异无显著性(P＞0.05).结论腹腔镜疝修补术是一种安全而有效的无张力疝修补手术,较Bassini组手术后恢复时间短,术后并发症少.     

腹腔镜联合腹股沟小切口治疗成人腹股沟疝20例

目的探讨在腹腔镜下行成人腹股沟疝未闭内环的高位结扎后,另行腹股沟小切口行疝修补的可行性与优越性。方法自2005年1月￣2006年6月在腹腔镜下先行未闭疝囊高位结扎再做腹股沟小切口行疝修补术(其中嵌顿疝2例、复发疝2例、双侧疝3例)。结果20例手术均成功,平均手术时间40min,术中无并发症,住院3d￣5d,平均4d。结论腹腔镜下行成人腹股沟疝未闭内环口高位结扎,再做腹股沟小切口行疝修补术,由于疝囊高位结扎确切,腹股沟不分离疝囊,患者创伤小,康复快,住院时间短,不易复发,无肠粘连等并发症。     

无张力疝修补术治疗老年腹股沟疝的临床分析

目的 评价无张力疝修补手术治疗老年人腹股沟疝的疗效。方法 回顾性分析105例老年腹股沟疝行无张力疝修补手术的临床资料。结果 105例老年患者共施行了123次无张力疝修补术，其中83次行疝环充填式无张力疝修补术，40次行Lichtenstein手术，单侧疝及双侧疝平均手术时间分别为36min，73min。术后并发症仅1例小的皮下血肿，无切H感染，无围手术期死亡。腹股沟疝患者均于术后3～9d痊愈出院。75％(79／105)病例获得随访，随访时间5～50个月，无术后复发，仅1例有腹股沟区疼痛。结论 采用无张力疝修补手术治疗老年人腹股沟疝是安全的，其并发症少，复发率低；对双侧腹股沟疝的患者可同期进行手术，避免再次手术。     

完全腹膜外腹腔镜疝修补术52例体会

目的探讨完全腹膜外腹腔镜下疝修补术治疗腹股沟疝的临床疗效。方法回顾性分析采用完全腹膜外腹腔镜下疝修补术治疗的52例腹股沟疝患者的临床资料。结果 52例患者中,2例中转腹腔腹膜前修补,完全腹膜外腹股沟疝修补成功率96.2%;平均手术时间72min,术后平均住院时间为6d;术后无伤口感染及血管、器官损伤;术后平均随访10个月,无1例疝复发。结论完全腹膜外腹腔镜下疝修补术无需进入腹腔,可减少并发症,为腹股沟疝合理、有效的外科术式。     

三种手术方法治疗成年人腹股沟疝的临床护理比较

目的:探讨和比较传统腹股沟疝修补术、无张力疝修补术及腹腔镜疝修补术的临床护理效果.方法:回顾性分析2000年~2010年采用传统方法修补腹股沟疝(传统组)231例,2002年后另采用无张力疝修补术(无张力组)150例及腹腔镜疝修补术(腹腔镜组)50例.比较3组患者术后恢复情况的相关指标.结果:传统组的平均手术时间为(70.2±20.1)min,无张力组为(50.6±15.8)min,腹腔镜组为(33±12.5)min.无张力组复发4例,复发率为2.66%;腹腔镜组为1例,复发率2%.结论:同传统腹股沟疝修补术比较,无张力修补术和腹腔镜疝修补具有住院时间短、痛苦小、复发率低的特点,腹腔镜疝修补手术区疼痛更轻,切口更美观,患者更满意.     

免固定腹腔镜全腹膜外疝修补术25例临床应用体会

目的：总结免固定腹腔镜全腹膜外疝修补术（TEP）临床应用体会和经验。方法：对25例从2008年1月起行腹腔镜完全腹膜外疝修补术治疗腹股沟疝患者的临床资料进行回顾性分析。结果：全部病例均成功施行腹腔镜全腹膜外疝修补术，单侧疝手术时间30．120min，平均为50min，平均4天出院。术后阴囊血肿1例，局部血清肿1例。随访至2010年3月，无复发病例。结论：免固定的TEP同样安全、可靠，并发症降低，且操作更为简单易行。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.