基因重组人骨形成蛋白—2与珊瑚／聚乳酸复合异位诱导成骨的实验研究

目的：观察基因重组人骨形成-2(rhBMP-2)和珊瑚／聚乳酸形成复合人工骨的异位诱导成骨活性。方法：把rhBMP-2和珊瑚／聚乳酸形成复合人工骨。进行小鼠肌内种植1，3，6周后，组织学观察其异位诱导成骨活性。结果：rhBMP-2赋予珊瑚／聚乳酸骨诱导能力，珊瑚／聚乳酸则充当rhBMP-2的载体和释放系统，对BMP的活性未不利影响。与单纯的珊瑚／聚乳酸相比，这种复合人工骨以软骨内成骨的方式诱导成骨。结论：rhBMP-2／珊瑚／聚乳酸复合人工骨具有良好的生物相容性和骨诱导活性，是一种较理想的骨移植替代材料。     

基因重组人骨形成蛋白-2与珊瑚/聚乳酸复合异位诱导成骨的实验研究

目的 :观察基因重组人骨形成蛋白 - 2 (rhBMP - 2 )和珊瑚 /聚乳酸形成复合人工骨的异位诱导成骨活性。方法 :把rhBMP - 2和珊瑚 /聚乳酸形成复合人工骨。进行小鼠肌内种植 1、3、6周后 ,组织学观察其异位诱导成骨活性。结果 :rhBMP - 2赋予珊瑚 /聚乳酸骨诱导能力 ,珊瑚 /聚乳酸则充当rhBMP - 2的载体和释放系统 ,对BMP的活性未产生不利影响。与单纯的珊瑚 /聚乳酸相比 ,这种复合人工骨以软骨内成骨的方式诱导成骨。结论 :rhBMP - 2 /珊瑚 /聚乳酸复合人工骨具有良好的生物相容性和骨诱导活性 ,是一种较理想的骨移植替代材料     

基因重组人骨形成蛋白-2和聚乳酸复合异位诱导成骨的实验研究

目的：观察基因重组人骨形成蛋白－2（recombinant human Bone Morphofenetic protein-2,rhBMP-2)和聚乳酸（Polylactic acid,PLA)复合形成的活性涂层种植体的异位诱导成骨活性。方法：将rhBMP-2与聚乳酸复合，构建活性涂层种植体，植入兔肌内，于1，4，8周后进行X线、大体观察及组织学观察，检测异位成骨活性。结果：rh-BMP-2/PLA活性复合涂层种植体具有骨诱导能力，PLA为rhBMP-2的良好控释载体。结论：rhBMP-2/PLA涂层具有良好的生物相容性和骨诱导活性，是一种理想的种植体涂层形成。     

重组人骨形成蛋白─2和珊瑚复合移植的超微结构观察

为了弥补单纯珊瑚无骨诱导活性、骨修复能力较差的缺陷，本研究将具有骨诱导特性的重组人骨形成蛋白-2（rhBMP－2）和珊瑚复合，制备出rhBMP-2／珊瑚复合人工骨。将其植入免颅骨标准大小缺损，以单纯珊瑚植入作对照；术后2、6、12周取材，通过X残片和扫描电镜观察，评价其骨修复能力。结果显示；rhBMP－2／珊瑚复合人工骨的骨修复能力和骨修复效果明显优于单纯的珊湖，是一种较为理想的骨移植替代材料。     

rhBMP-2/PLA复合涂层种植体骨内诱导成骨的实验研究

目的：观察基因重组人骨形成蛋白-（recombinanthuman bone morphogenetic protein-2，rhBMP-2）和聚乳酸（polylactic acid，PLA）复合形成的活性涂层种植体骨内诱导成骨活性。方法：将rhBMP-2与聚乳酸复合构建活性涂层种植体，植入兔股骨内，分别于4，8，12，16周处死动物，进行组织学及扫描电镜及组织化学观察，检测其成骨活性。结果：构建的活性涂层种植体具有骨诱导能力，PLA为rhBMP-2的良好控释载体。结论：rhBMP-2/PLA涂层具有良好的生物相容性和骨诱导活性，是一种理想的种植体形式。     

以胶原缓释rhBMP-2复合BMSCs及珊瑚构建组织工程骨异位成骨的实验研究

目的：观察以胶原缓释rhBMP-2复合BMSCs及珊瑚构建的组织工程骨异位成骨的能力。方法：构建3种复合支架材料：1)rhBMP-2／珊瑚；2)胶原rhBMP-2／珊瑚；3)BMSCs／胶原rhBMP-2／珊瑚。分别植入裸鼠皮下，8周后观察成骨情况，并作比较。结果：第3组材料异位成骨的能力最强，第2组次之，第1组较弱。结论：动物实验中胶原是rhBMP-2适宜的缓释载体，BMSCs对促进材料异位成骨有重要意义。     

基因重组人骨形成蛋白-2与珊瑚/聚乳酸复合修复骨质缺损的实验研究

目的　观察生物珊瑚、聚乳酸和rhBMP-2合成人工骨(复合骨)修复兔颅骨缺损后复合骨的变化情况。方法　选择24只新西兰兔,随机分成2组,每组12只,建立兔颅骨缺损标准模型。植入复合骨,用珊瑚/聚乳酸作为对照。术后4、8、12周每组各处死4只动物,取出植入体进行扫描电镜观察和机械强度测定。结果　复合骨在植入缺损后,不仅在植入体周边部有骨组织长入,而且在整个植入体内均有新骨形成,即出现多中心成骨。复合骨在同一时间点的成骨量明显多于对照组,随时间推移,成骨量递增。在植入前,两材料间的抗压强度无明显差异;在植入后,两植入体的抗压强度则差异显著,复合骨明显高于同期的对照组。结论　生物珊瑚、聚乳酸和rhBMP-2合成人工骨在体内以传导成骨和诱导成骨双重机制完成骨修复,且有良好的机械强度,作为植骨材料具有良好的应用前景。     

应用珊瑚作为重组人骨形成蛋白-2缓释载体的动物实验研究

将大肠杆菌表达的重组人骨形成蛋白-2（rhBMP－2）和珊瑚（coral）复合，制成rhBMP－2／coral复合人工骨，将其植入小鼠股部肌肉内，并以单纯珊瑚植入作对照，术后1、3、6周取材，组织学观察。结果显示：rhBMP－2／coral复合人工骨在植入局部诱导形成骨和软骨，表现出良好的异位诱导成骨能力；珊瑚对rhBMP－2的活性未产生不利影响，是rhBMP－2的良好缓释载体。此复合人工骨可望作为一种新型生物性植骨材料而应用于骨科和颌面外科。     

rhBMP-2、胶原和珊瑚复合人工骨在不同种类动物体内异位诱导新骨形成的实验研究

:目的:研究rhBAAP2,胶原,珊瑚复合骨在于同种属动物体内诱导异位成骨的活性。材料与方法:本研究采用肌袋异位成骨的动物模型,通过大体、组织学观察、组织测量等手段,研究珊瑚、型胶原、rhBMP2复合骨在大鼠、兔、狗体内异位诱导新骨形成的特点,珊瑚降解的过程。结果:rhBMP2、珊瑚和胶原复合骨在大鼠、兔、狗体内均可异位诱导骨组织形成,在大鼠体内诱导成骨量大,骨组织成熟程度高,在兔、狗体内成骨量小,成熟程度低。结论:说明rhBMP2、胶原、珊瑚复合骨在不同种属动物体内都具有异位诱导骨组织形成的活性。     

rhBMP-2、胶原、珊瑚复合骨诱导骨形成生物活性的实验研究

目的：研究珊瑚、胶原、ｒｈＢＭＯＰ－２复合骨异位诱导新骨形成的活性和载体在体内降解情况。方法：选择珊瑚和１型胶 为ｒｈＢＭＰ－２载体,采用肌袋异位成骨的动物模型,通过大体观察、组织测量等手段,研究复合骨诱导新骨形成的特点、珊瑚降解的过程。结果：ｒｈＢＭＰ－２、珊瑚和胶原复合骨诱导骨组织形成,在一定范围内随作用时间延长新骨形成量持续增加,其中ｒｈＢＭＰ－２、胶原、珊瑚复合骨具有骨引导性和骨诱导性,珊     

Experimental tooth movement into bone induced by recombinant human bone morphogenetic protein-2.

OBJECTIVE: The development of biomaterials for secondary bone grafting to the alveolar cleft, recombinant human morphogenetic protein-2 (rhBMP-2) may have great potential to promote osseous regeneration. The purpose of this study was to investigate the osteoinductive activity of low-dose rhBMP-2 (10 microg/100 microL) and the biological response of newly generated bone to orthodontic stimulation, compared with previous results using high-dose rhBMP-2 (40 microg/100 microL). METHOD: After extraction of the maxillary first premolars, bone defects were surgically created in eight adult beagle dogs using a 5-mm-diameter trepan bar. The rhBMP-2 with a PLGA/gelatin sponge complex (PGS) made of gelatin and polylactic acid polyglycolic acid copolymer was immediately implanted. The osteoinductive activity of rhBMP-2 and tooth movement into the newly generated bone was evaluated based on histological and morphometric examination. RESULTS: Dramatic histological and morphometric differences were found in the time course of bone remodeling between low- and high-dose rhBMP-2. The onset of bone remodeling occurred significantly earlier in low-dose rhBMP-2. During experimental tooth movement, in contrast to high-dose rhBMP-2, with which root resorption occurred, only partial cementum resorption occurred on the pressure side with low-dose rhBMP-2. CONCLUSION: These results indicated that the proper dosage of rhBMP-2 should be determined for successful clinical application.     

提取并部分纯化bBMP促进节段性骨缺损愈合的定量组织学研究

目的　从小牛骨基质中提取并部分纯化bBMP ,以促进节段性骨缺损的愈合。方法　通过SDS PAGE电泳及生物活性鉴定实验 ,证实bBMP保留了其活性蛋白成分 ,具有良好的骨诱导活性。在此基础上 ,合成一种新型复合人工骨材料即HA bBMP Co ,用以整复家兔尺骨中段 1.5cm节段性骨缺损 ,并对缺损区新骨生长量进行定量组织学测定。结果　HA bBMP Co复合人工骨植入整复节段性骨缺损 ,其新骨生长量明显高于同期HA Co复合人工骨组 (P <0 .0 1)。结论　HA bBMP Co复合人工骨能有效地促进节段性骨缺损的愈合 ,是一种具有良好应用前景的骨替代材料     

rhBMP-2复合生物活性玻璃诱导成骨的实验研究

目的：探讨生物活性玻璃(bioactive glass BG)对重组人骨形成蛋白-2(recombinant human bone morphogenetic protein-2,rhBMP-2)诱导骨形成的影响。方法：6只大鼠皮下袋植入rhBMP-2／BG,0．2mg 0．5mg rhBMP-2,并设空白对照组。植入后3周行组织学检查。结果：0．5mg组rhBMP-2／BG具有成骨作用,其骨化过程类似于膜内化骨的直接骨形成。结论：rhBMP-2／BG复合物能诱导新骨形成,表明BG适合作为rhBMP-2载体。     

Experience with freeze-dried PGLA/HA/rhBMP-2 as a bone graft substitute.

We investigated bone induction by recombinant human bone morphogenetic protein-2 in rodents. The purpose of this study was to evaluate the osteoinductive potential of a resorbable bone substitute fabricated from freeze-dried poly(glycolic acid-co-lactic acid) (PGLA) mixed with hydroxyapatite particles incorporated with bone morphogenetic protein-2 in skull defects of rats (FD-PGLA/HA/rhBMP-2). The FD-PGLA/HA/rhBMP-2 composite or as a control, the FD-PGLA/HA by itself were implanted in skull defects (psi 8 mm) of rats. The samples were harvested at 2 or 4 weeks postoperatively and were studied radiographically and histologically. Four weeks after implantation, the FD-PGLA/HA/rhBMP-2 discs were completely replaced by newly-formed bone possessing bone marrow. In contrast, the defects implanted with FD-PGLA/HA were filled only with fibrous connective tissue. The results suggest that the FD-PGLA/HA/rhBMP-2 composite could be an optimum bone substitute with osteoinductive potential and could function as an alternative bone graft material for autogenous bone in humans.     

A histological study on experimental tooth movement into bone induced by recombinant human bone morphogenetic protein-2 in beagle dogs.

OBJECTIVE: The purpose of this preliminary study was to examine experimental tooth movement into newly generated bone induced by recombinant human bone morphogenetic protein-2 (rhBMP-2). METHOD: After extraction of the maxillary first premolars, bone defects were surgically created in eight adult beagle dogs using a 5-mm-diameter trepan bar. According to which material was grafted into the bone defects, animals were divided into the following four groups: (1) the rhBMP-2 group in which rhBMP-2 with a poly[ D,L-(lactide-co-glycolide)]/gelatin sponge complex was implanted; (2) the spongiosa group in which spongiosa from the tibia was grafted; (3) the nongrafted group in which no material was embedded; and (4) the control group in which only tooth extraction was performed. The osteoinductive activity of rhBMP-2 and tooth movement into the newly generated bone were examined by histological and morphometric comparisons of each group. RESULTS: Considerable new bone formation was observed at the grafted site both in the rhBMP-2 and in the spongiosa groups. The area of generated bone in the rhBMP-2 group was significantly greater than that in the spongiosa group. Newly generated bone, in both the rhBMP-2 and spongisosa groups, showed a similar histological response to orthodontic force as in normal alveolar bone in the control group. However, root resorption occurred on the pressure side in the rhBMP-2 group. CONCLUSION: These results indicated that rhBMP-2 might constitute an alternative material to autogeneous bone grafting for alveolar cleft defects. Further studies regarding tooth movement into generated bone induced by rhBMP-2 are suggested.     

重组人骨形成蛋白-2/珊瑚复合人工骨的动物实验研究

本研究将重组人骨形成蛋白－２（ｒｈＢＭＰ－２）和珊瑚以一定的方式复合后,植入小鼠股部肌袋和兔颅骨标准大小缺损,以单纯珊瑚植入作对照,术后不同时间取材,通过组织学方法检测其骨诱导活性和骨修复能力．结果显示：ｒｈＢＭＰ－２／珊瑚复合人工骨植入小鼠肌袋１周,诱导软骨形成,３周,形成编织骨,６周,形成含骨髓的板层骨,同时,珊瑚被部分降解吸收;复合人工骨植入兔颅骨缺损后,以引导成骨和诱导成骨双重机制完成骨修复过程,术后１２周,植入物完全被成熟的骨组织取代,其骨修复效果明显优于单纯的珊瑚．此复合人工骨具有骨传导和骨诱导活性,骨修复能力较强,是一种较理想的新型生物性植骨材料     

Bone augmentation by recombinant human BMP-2 and collagen on adult rat parietal bone.

A composite of recombinant human bone morphogenetic protein-2 (rhBMP-2) and collagen was implanted beneath the cranial periosteum of 10-month-old rats to observe bone development and absorbent change of carrier collagen. The rhBMP-2/collagen onlay implant resulted in active bone formation and the augmented bone was connected directly with the original bone, whereas the collagen alone resulted in neither bone nor cartilage. The ossification process in the rhBMP-2/collagen occurred directly through bone formation, similar to intramembranous ossification. The carrier collagen fibers were found in the woven bone and were completely absorbed at 8 weeks in the presence of rhBMP-2, while the collagen alone implant remained encapsulated by a thin, fibrous connective tissue. Our results indicate that rhBMP-2/collagen is an effective material as a biological onlay implant, showing osteoinductive properties and being completely replaced by new bone. Carrier collagen not only plays a role in rhBMP-2 delivery, but also provides a cell anchorage for cell differentiation and remains as an artificial matrix in woven bone.