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1.
Increased incidence of numerical chromosome abnormalities in spermatozoa injected into human oocytes by ICSI 总被引:6,自引:0,他引:6
The potential risk of transmitting chromosomally abnormal spermatozoa from infertile males into oocytes through intracytoplasmic sperm injection (ICSI) has prompted us to investigate the male pronuclei of tripronuclear zygotes (3PN) obtained after ICSI. To specify the type of anomalies, we used triple colour fluorescent in-situ hybridization (FISH) with three specific probes for chromosomes X, Y and 18. From a total of 163 paternal complements of ICSI-3PN zygotes, 90 (55.2%) had Y-chromosome signals. Eighty-three of these were normal, four had the disomy XY and three were diploid. In the remaining 73 ICSI-3PN zygotes without Y-chromosome signals, the origin of paternal pronuclei was extrapolated through chromosome constitution of the first polar body. Five anomalies were found in this group of zygotes, giving a total rate of numerical chromosome aberrations for fertilizing spermatozoa of 7.4%. In contrast to ICSI, only two disomies (1.5%) were found in the control group of IVF-3PN zygotes. Compared with the incidence of chromosome anomalies between paternal-derived pronuclei of ICSI- and IVF-3PN zygotes, the difference was statistically significant (P < 0.025). This study provides the first direct evidence of a higher incidence of numerical chromosome anomalies in sperm-fertilized human oocytes after ICSI. 相似文献
2.
Cytogenetic analysis of unfertilized human oocytes 总被引:3,自引:1,他引:3
R R Angell W Ledger E L Yong L Harkness D T Baird 《Human reproduction (Oxford, England)》1991,6(4):568-573
Cytogenetic studies were carried out on 180 oocytes that appeared unfertilized after in-vitro fertilization. The majority of the 135 that were informative had grossly haploid second meiotic metaphases, two were grossly diploid, and five had a variety of different abnormalities. Twenty-one oocytes were abnormally fertilized and included prematurely condensed sperm chromosomes. The frequency of this phenomenon varied according to the stimulation protocol, those oocytes maturing longer in vivo showing less propensity to abnormal fertilizations. Thirteen per cent of the analysable haploid metaphases were hyperhaploid but none contained extra whole chromosomes. The extra components were a single chromatid (one case), or two single chromatids replacing a whole chromosome (four cases). The data suggest that the chromatids arose as a result of premature centromere division at meiosis I, and that this may be a major mechanism for trisomy formation rather than non-disjunction of whole bivalents at meiosis I, as generally believed. 相似文献
3.
The behaviour of sperm cells after intracytoplasmic sperm injection(ICSI) was investigated by analysing 192 unfertilized and 37one-pronuclear (1PN) oocytes following ICSI. Eighty-two unfertilizedoocytes were directly fixed whereas 110 were first parthenogeneticallyactivated by puromycin. In contrast to the findings in unfertilizedoocytes after in-vitro fertilization, most unfertilized oocytesafter ICSI (n = 76) contained evidence of the presence of spermatozoain the cytoplasm. Few oocytes (n = 6) contained prematurelycondensed sperm chromosomes (PCC), whereas the majority containedeither intact sperm heads (n = 31) or swollen sperm nuclei (n= 39) along with metaphase II chromosomes of the oocyte. Followingactivation by puromycin, swollen sperm nuclei and PCC were nolonger observed, whereas unchanged sperm heads persisted in12 oocytes displaying a single pronucleus. A non-decondensedsperm nucleus along with decondensed maternal chromatin werealso discovered in 32 out of 37 oocytes displaying a singlepronucleus after ICSI. The findings in unfertilized and 1PNoocytes after ICSI indicate that successful sperm injection,even followed by oocyte activation, is not sufficient to guaranteenormal fertilization. It seems that partial sperm membrane damageprior to injection is also required to ensure normal sperm decondensation. 相似文献
4.
Chromosomal analysis of multipronuclear zygotes obtained after partial zona dissection of the oocytes 总被引:2,自引:0,他引:2
We have attempted to analyse the chromosome constitution of multipronuclear
1-cell zygotes obtained after partial zona dissection (PZD) of the oocytes.
Six cells with three pronuclei could not be evaluated whereas another one
was characterized by the presence of a normal haploid and two
uninterpretable metaphases. Complete karyotypes were established for 21
tripronuclear cells, taking the varying arrangement of the chromosome sets
into consideration. Of the zygotes, 10 showed three separated haploid
metaphases (distribution pattern n/n/n), eight zygotes had one haploid and
one diploid chromosome set (n/2n) and in three cells the individual sets
were not distinguishable (3n). The sex chromosome ratio XXX:XXY:XYY was
7:9:5. Chromosome abnormalities were found in eight of the completely or
partially analysable tripronuclear zygotes (36.4%) and included numerical
(4 cells), structural (2 cells) as well as combinations of numerical and
structural alterations (2 cells). Three out of 11 zygotes with four
pronuclei could not be evaluated at all. In three cases, only two
chromosome sets were analysable and another cell displayed one
uninterpretable set. Three out of eight completely or partially analysable
zygotes with four pronuclei (37.5%) had chromosomal abnormalities.
Excluding the four cells with one or two uninterpretable metaphases, the
sex chromosome distribution XXXX:XXXY:XXYY:XYYY in the zygotes with four
pronuclei was 0:1:1:2. Compared with previously analysed multipronuclear
zygotes obtained after conventional in-vitro fertilization (IVF), the rate
of aberrant zygotes as well as the incidence of aberrant (male + female)
chromosome sets were not significantly changed after PZD.
相似文献
5.
Pellestor F Andréo B Arnal F Humeau C Demaille J 《Human reproduction (Oxford, England)》2002,17(8):2134-2145
BACKGROUND: Although numerous studies have been published on the chromosomal constitution of in-vitro unfertilized human oocytes, data remain highly variable and controversial because of the size of oocyte samples, technical reservations and potential misinterpretation. METHODS: A cytogenetic study was undertaken on 3042 unfertilized human oocytes recovered from 792 women participating in an IVF programme for various infertility problems. Both a gradual fixation technique and an R-banding procedure were used. RESULTS: The analysis was successful in 1397 oocytes (45.9%) for which interpretable metaphases were obtained. Of the 1397 oocyte karyotypes, 1088 (77.9%) were normal (23,X). The overall frequency of chromosomal abnormality was 22.1%. No correlation was found between the rate of abnormalities and the type of infertility. Aneuploidy was observed in 151 cells (10.8%), consisting of 5.4% hypohaploidies, 4.1% hyperhaploidies, 0.8% complex aneuploidies and 0.05% extreme aneuploidies with less than 18 chromosomes. Both whole chromosome non-disjunction and chromatid predivision contributed to the formation of aneuploid oocytes, but the numerical abnormalities due to single chromatids significantly exceeded conventional non-disjunctions. Abnormalities also included 5.4% diploid oocytes, 3.8% sets of chromatids alone and 2.1% structural aberrations. Aneuploidy was found in all chromosome groups. However, groups E and G exhibited significantly higher frequencies of non-disjunction than expected, whereas groups A and B showed a significantly low incidence of aneuploidy. CONCLUSIONS: The implication of both chromosome and chromatid abnormalities in the occurrence of non-disjunction are discussed in relation to the recent data on chromatid cohesion throughout cell division. The results were consistent with the hypothesis of an unequal occurrence of non-disjunction among the chromosome groups in female meiosis. 相似文献
6.
Kuleshova L Gianaroli L Magli C Ferraretti A Trounson A 《Human reproduction (Oxford, England)》1999,14(12):3077-3079
We report the birth of a healthy baby girl at 37 weeks gestation to a 47 year old recipient, after vitrification of mature oocytes from four in-vitro fertilization (IVF) patients. A total of 17 oocytes was vitrified in 1-2 microl of ethylene glycol (40%) and 0.6 mol/l sucrose (20.54%) in open pulled straws. Eleven oocytes survived after vitrification and five pronuclear zygotes were obtained after intracytoplasmic sperm injection (ICSI). Three embryos were transferred to three patients, two of whom were the original oocyte donors and pregnancy was not established. The third embryo was donated to a 47 year old infertile woman after preimplantation diagnosis had confirmed euploidy for chromosomes X, 13, 14, 15, 16, 18, 21 and 22. The successfully completed pregnancy is encouraging for further research to explore the potential benefits of vitrification for the cryopreservation of human oocytes, given the relatively low success of conventional freezing of human oocytes by slow cooling methods. 相似文献
7.
Tsuchiya K Kamiguchi Y Sengoku K Ishikawa M 《Human reproduction (Oxford, England)》2002,17(2):420-425
BACKGROUND: The purpose of this study was to investigate the chromosomal complement and developmental potential of in-vitro matured murine oocytes following ICSI by human sperm. METHODS: Heterologous ICSI fertilization between mouse oocytes and human sperm was employed in order to overcome the reduced fertilization rates observed after conventional IVF due to zona hardening during in-vitro maturation, and to assess separately maternal and paternal chromosome complements. Cytogenetic analyses were performed in four types of oocytes: (i) in-vitro matured metaphase II (MII) oocytes; (ii) in-vivo matured MII oocytes; (iii) in-vitro matured oocytes after ICSI; (iv) in-vivo matured oocytes after ICSI. RESULTS: Activation rates after ICSI of in-vitro matured oocytes was lower than that of in-vivo matured oocytes (69.9 versus 97.2%, P < 0.01), and premature chromosomal condensation was only observed in in-vitro matured oocytes. However, there were no significant differences in developmental rates after successful activation between in-vivo and in-vitro matured ICSI oocytes (69.7 versus 76.6%). The incidences of aneuploidy and structural aberrations were similar between the ICSI embryos and non-ICSI (MII) oocytes. Furthermore, the frequency of chromosomal aberrations was not associated with in-vitro or in-vivo maturation. Similar analyses of paternal chromosomes indicated that there were no significant differences in the incidence of chromosomal aberrations between the embryos derived from in-vitro and in-vivo matured oocytes. CONCLUSIONS: These results suggest that in-vitro matured oocytes following ICSI do not lead to an increase in the frequency of aneuploidy and structural aberrations when human sperm are injected into mouse oocytes. 相似文献
8.
Heindryckx B Lierman S Van der Elst J Dhont M 《Human reproduction (Oxford, England)》2004,19(5):1189-1194
BACKGROUND: Infertility due to the absence of gametes is one of the last frontiers in reproductive medicine. Sperm or oocyte donation is currently the only treatment option but this approach lacks the genetic contribution of both partners. Artificial production of gametes through haploidization may offer an alternative strategy. The aim of this study was to evaluate the efficiency of producing artificial oocytes and zygotes with correct chromosome number. METHODS AND RESULTS: Somatic cumulus cell nuclei were injected into non-enucleated oocytes to produce artificial zygotes and into enucleated mature mouse oocytes to produce artificial oocytes. The expected chromosome number of artificial zygotes and oocytes is 40 and 20 chromosomes respectively. Fertilization and developmental potential of artificial zygotes and oocytes inseminated by IVF or ICSI were investigated. The expected chromosome numbers were found in 12% of artificial zygotes and 15% of artificial oocytes. Varying the time interval between injection of the somatic nucleus and activation (3, 5, 8 h) tended to increase the efficiency up to 18 and 23% for zygotes and oocytes respectively. Two-cell formation rates were 90% for artificial zygotes and 37% for artificial oocytes after IVF and 53% for artificial oocytes after ICSI. Blastocyst formation rates were 15, 8 and 9% respectively. CONCLUSIONS: Chromosome number analysis shows that the efficiency of obtaining artificial zygotes and oocytes with correct chromosome number was low and that developmental potential was severely hampered. These observations question the possibility of obtaining chromosomally normal embryos from artificial oocytes or zygotes. 相似文献
9.
The meiotic competence of in-vitro matured human oocytes is influenced by donor age: evidence that folliculogenesis is compromised in the reproductively aged ovary 总被引:11,自引:13,他引:11
Volarcik K; Sheean L; Goldfarb J; Woods L; Abdul-Karim FW; Hunt P 《Human reproduction (Oxford, England)》1998,13(1):154-160
The human oocyte appears to be particularly prone to meiotic errors, and
the incidence of these errors is strongly influenced by maternal age. We
have initiated studies of human oocytes from unstimulated ovaries and have
observed age-related effects on the meiotic process in oocytes from
unselected antral follicles. Specifically, in oocytes obtained from donors
over the age of 35 years, the majority of oocytes that extruded a first
polar body in culture and arrested at second meiotic metaphase had
aberrations in spindle formation and chromosome alignment. Similarly,
observations of a limited number of oocytes at first meiotic metaphase
suggest disturbances at this stage of meiosis as well. Finally, preliminary
results of non-disjunction studies suggest that the frequency of errors in
chromosome segregation at the first meiotic division is influenced by donor
age in in-vitro matured oocytes as it is in oocytes undergoing meiotic
maturation in vivo. These data provide direct evidence that the meiotic
competence of oocytes from unstimulated ovaries declines with donor age.
Similarly, studies of in-vitro fertilization (IVF) pregnancies in older
women indicate that the developmental competence of the human oocyte
declines with age. Since both meiotic and developmental competence are
acquired during the late stages of oocyte growth, we postulate that an age-
related decline in the process of folliculogenesis results in reduced
oocyte quality and that the well characterized age-related increase in
meiotic non-disjunction is one symptom of compromised oocyte growth.
相似文献
10.
Abnormalities of sperm chromosome condensation in the cytoplasm of immature human oocytes 总被引:1,自引:1,他引:1
M T Zenzes C de Geyter J Bordt H P Schneider E Nieschlag 《Human reproduction (Oxford, England)》1990,5(7):842-846
This study analysed data from 27 couples in an IVF-ET programme. The maternal age range was 28-43 years. Statistical analyses on 182 oocytes showed no maternal age effect on the number of oocytes, their stage of maturation or their fertilization rate. There was also no effect of age of either partner or of seminal parameters on the fertilization rate. In contrast, occurrence of diploid oocytes was confined to three of the older women. The proportion of failures of fertilization was significantly higher in immature oocytes. These failures, which included 18 uncleaved, multipronuclear or fragmented zygotes, were related to disturbances of oocyte maturation. Four (out of five) oocytes re-inseminated with fresh semen produced polyspermy. One zygote showed marked asynchrony in the development of the two pronuclei. In eight zygotes the paternal complements had an allocyclic pattern of chromosome condensation between and within chromosomes or chromosome regions. In two other zygotes the paternal complement showed one chromosome prematurely condensed. This single-chromatid chromosome would be lost in the following cleavage division, suggesting that aneuploidy due to 'anaphase lag' is not a rare event during embryo cleavage. 相似文献
11.
Wall M.B.; Marks K.; Smith T.A.; Gearon C.M.; Muggleton-Harris A.L. 《Human reproduction (Oxford, England)》1996,11(10):2230-2238
This study was undertaken to establish baseline data on thechromosomal status of failed-fertilized oocytesderived from in-vitro fertilization (IVF) or intracytoplasmicsperm injection (ICSI) procedures. A cytogenetic analysis wasundertaken on 162 IVF and 51 ICSI oocytes. In all, 82.1% (133/162)of the IVF and 78.4% (40/51) of the ICSI oocytes had metaphaseII (Mil) plates, of which 50.4% of the IVF and 47.5% of theICSI oocytes were analysed further. Chromosomes of the G-group(2122) were identified with the majority of the anomalies.No overall significant difference in the aneuploidy rate wasfound for the IVF (37.3%) or ICSI (31.6%) oocytes, or with maternalage. However, chromosome anomalies, e.g. diploidy, fragmentedand broken chromatids, single sperm and oocyte chromatids, werefound in oocytes from IVF patients aged >36 years and inthe ICSI oocytes throughout the maternal age range (3138years). The status of the polar body chromatin indicated thatthere was no overall significant difference in the maturationof the IVF and ICSI oocytes. Evidence of successful sperm deliverywas found in 72.5% (37/51) of the ICSI failed-fertilized oocytes.In this group there was a significant increase in the incidenceof premature chromosome condensation: 19.6% (10/51) containedsperm chromosomes, 7.8% (4/51) had swollen sperm heads, andthe remaining 45.0% had condensed sperm heads. The presenceof both sperm and Mil oocyte chromosomes was found in 19.6%(10/51) of the ICSI and 8.6% (14/162) of the IVF failed-fertilizedoocytes. Specific fluorescent in-situ hybridization DNA probeswere used to re-analyse the chromosomes of karyotyped failed-fertilizedIVF oocytes and, for the first time, applied to the karyotypedchromosomes of failed-fertilized ICSI oocytes. The hybridizationefficiency was 8695% for the centromere probe and 100%for probes 21 and 18. 相似文献
12.
F Pellestor 《Human reproduction (Oxford, England)》1991,6(9):1252-1258
The cytogenetic study of human gametes is a new and important source of information because most chromosomal abnormalities originate from meiotic disorders. The frequency and type of abnormalities were analysed in both spermatozoa and mature oocytes. A total of 13,975 human sperm chromosome complements and 1897 oocyte chromosome complements were analysed. In the present study, pooled cytogenetic data on human gametes have been examined to determine and compare the distribution of non-disjunctions in male and female gametes. Human spermatozoa are characterized by a significant excess of hypohaploidy and an equal distribution of aneuploidies among all chromosome groups, whereas mature oocytes display an equal ratio of hypohaploidy to hyperhaploidy and a high variability in the distribution of non-disjunction: in particular, there is a significant over-representation of aneuploidies in both D and G chromosome groups. This indicates that non-disjunction is not a random event in female meiosis and, consequently, that there are differences in the meiotic process between the sexes. Meiotic and environmental factors which could explain the non-random malsegregation of chromosomes in female meiosis are discussed. The role of maternal age as a cause of aneuploidy is questioned. 相似文献
13.
Rosenbusch B 《Human reproduction (Oxford, England)》2006,21(11):2739-2742
Valuable information on the cytogenetic constitution of female gametes has been deduced from the direct, so-called conventional analysis of oocytes remaining unfertilized in programmes of assisted reproduction. Additional, indirect conclusions have become possible by PGD of the polar bodies. Both techniques provided evidence for the co-existence of two aneuploidy-causing mechanisms during first maternal meiosis; non-disjunction (ND) of bivalents results in the loss or gain of whole chromosomes in metaphase II complements, whereas a precocious division (pre-division, PD) of univalents leads to the loss or gain of single chromatids. As to the distribution of ND and PD, however, direct oocyte chromosome studies and PGD tell surprisingly different stories. Moreover, first and second polar body analyses contradict the data derived from DNA polymorphism studies concerning the distribution of first and second meiotic division errors. An increased awareness of these problems appears necessary because important decisions are made on the basis of PGD results. 相似文献
14.
The chromosomal constitution of multipronuclear zygotes resulting from in-vitro fertilization 总被引:1,自引:4,他引:1
We have attempted to analyse the chromosome constitution of 77
multipronuclear uncleaved zygotes obtained from our in-vitro fertilization
programme. Complete karyotypes could be established for 51 tripronuclear
cells and eight zygotes with four pronuclei. When compiling the results,
the varying arrangement of the chromosome sets was taken into
consideration. Eighteen tripronuclear zygotes showed three separate haploid
metaphases (distribution pattern n/n/n), 16 cells had one haploid and one
diploid chromosome set (n/2n), and in 15 zygotes the individual sets were
not distinguishable (3n). Two zygotes were in fact tetraploid, the
distribution of metaphases on the slide being n/3n and n/n/2n,
respectively. In tripronuclear zygotes the sex chromosome ratio XXX:XXY:XYY
was 14:16:18, excluding the two tetraploid cells and one zygote with a
23,X/23,X/22,-C or -Y karyotype. Chromosome abnormalities were found in 16
zygotes (31.4%) and included numerical (six cells), structural (four cells)
as well as combinations of numerical and structural alterations (six
cells). Four of the zygotes with four pronuclei (50%) had numerical and/or
structural chromosome aberrations. Excluding two cells with one
uninterpretable metaphase and a 22,-C or -Y karyotype, respectively, the
sex chromosome distribution XXXX:XXXY:XXYY:XYYY was 1:1:2:1 in zygotes with
four pronuclei. Another zygote was found to be pentaploid after fixation.
These results suggest that analysis of multipronuclear zygotes yields
valuable information about cytogenetic abnormalities occurring at the
earliest stage of conception.
相似文献
15.
B Rosenbusch M Schneider R Kreienberg C Brucker 《Human reproduction (Oxford, England)》2001,16(11):2362-2367
BACKGROUND: Digynic zygotes with three pronuclei and one polar body obtained after intracytoplasmic sperm injection (ICSI) were studied cytogenetically to elucidate the frequency and origin of chromosomal abnormalities at the earliest stage of conception. METHODS: Uncleaved, single-cell zygotes were incubated with podophyllotoxin and vinblastine and fixed by a gradual fixation air drying method. The chromosomes were stained with Giemsa. RESULTS: Twenty-two (50%) out of 44 informative zygotes revealed cytogenetic alterations, including aneuploidy (six cells, 13.6%), structural aberrations (10 cells, 22.7%) and combinations of numerical and structural abnormalities (two cells, 4.5%). In one case (2.3%), double aneuploidy or an effect of chromosomal translocation could not be distinguished and one zygote (2.3%) turned out tetraploid due to injection of a diploid spermatozoon. Two zygotes (4.5%) showed an irregular chromatid segregation between the two maternal complements. In completely analysable cells, the sex chromosome ratio XXX:XXY was 17:15. CONCLUSIONS: Digynic ICSI zygotes carry a high rate of cytogenetic abnormalities that obviously have been transmitted by the participating oocytes and spermatozoa. We also confirmed the previously reported, possibly ICSI-induced irregular oocyte chromatid segregation. The results suggest that aneuploidy in the oocytes must have been caused by predivision instead of non-disjunction. 相似文献
16.
Reconstruction of mouse oocytes by germinal vesicle transfer: maturity of host oocyte cytoplasm determines meiosis. 总被引:6,自引:0,他引:6
H Liu C W Wang J A Grifo L C Krey J Zhang 《Human reproduction (Oxford, England)》1999,14(9):2357-2361
We evaluated the maturational competence of mouse oocytes reconstructed by the transfer and electrofusion of germinal vesicles (GV) into anuclear cytoplasts of GV stage oocytes (both auto- and hetero-transfers), metaphase II stage oocytes or zygotes. Following in-vitro culture, the maturation rates of the reconstructed oocytes to metaphase II did not significantly differ between auto- and hetero-transfers (40/70 versus 95/144 respectively); these rates also did not differ from those of control oocytes (57/97) which were matured in vitro without micromanipulation and electrofusion. In contrast, when a GV was transferred into an enucleated metaphase II oocyte or a zygote, only a few reconstructed oocytes underwent germinal vesicle breakdown (5/30 and 2/21 respectively); moreover, none reached metaphase II stage. Cytogenetic and immunofluorescence analyses were conducted on hetero-GV oocytes that extruded a first polar body. Each oocyte showed two groups of chromosomes, one in the cytoplast and one in the polar body, as well as a bipolar spindle with twenty univalent chromosomes. Our findings suggest that oocytes reconstructed by GV transfer into a cytoplast of the same developmental stage mature normally in vitro through metaphase II. Such oocytes may be a useful research model to elucidate the cytoplasmic and nuclear mechanisms regulating meiosis and the relationships between meiotic errors and age-related changes in the oocyte. 相似文献
17.
A detailed cytogenetic analysis of large numbers of fresh and frozen-thawed human sperm after ICSI into mouse oocytes 总被引:2,自引:0,他引:2
Watanabe S 《Human reproduction (Oxford, England)》2003,18(6):1150-1157
BACKGROUND: Since information about chromosome aberrations in micro-manipulated sperm is still inadequate, cytogenetic analysis was performed on large numbers of fresh and frozen-thawed (FT) human sperm after injection into mouse oocytes. The effects of the ICSI procedure on oocytes are also discussed based on analysis of the mouse chromosome complements. METHODS: After the injection of fresh and FT human sperm into mouse oocytes, chromosomes of the hybrid oocytes were analysed at first cleavage metaphase. RESULTS: Incidences of the hybrid oocytes at the first cleavage metaphase were significantly different between fresh (71.5%) and FT sperm groups (80.1%) (P < 0.05). The chromosome analysis of 477 fresh and 141 FT sperm showed no difference in the incidences of aneuploidy (1.6/0.7%), structural aberrations (8.8/7.8%) or diploidy (0.0/0.0%) between these categories. The cytogenetic result did not differ from our previous result using IVF between human sperm and hamster oocytes. In an additional cytogenetic study on 615 mouse chromosome complements, the incidence of diploidy (5.4%) was significantly higher than those (0.3-2.8%) in the previous mouse cytogenetic studies, and the hybrid oocytes with no mouse chromosomes (2.0%) existed. CONCLUSIONS: This result suggests that the ICSI procedure induces no sperm chromosome aberrations but increases numerical aberrations in oocyte chromosome complements. 相似文献
18.
A De Vos H Van de Velde H Joris A Van Steirteghem 《Human reproduction (Oxford, England)》1999,14(7):1859-1863
About 4% of all the oocytes denuded prior to intracytoplasmic sperm injection (ICSI) are in metaphase-I (MI). Frequently, these oocytes achieve meiosis after a few hours of in-vitro culture and are available for ICSI on the day of oocyte retrieval. In this retrospective study, the aim was to evaluate the fertilization rate and the developmental capacity of these in-vitro matured MI oocytes. After controlled ovarian stimulation using human menopausal gonadotrophin (HMG) and human chorionic gonadotrophin (HCG) in 896 ICSI cycles, 1210 MI-to-MII-matured oocytes were injected approximately 4 h after in-vitro culture and 8803 MII oocytes were injected immediately, or later, after denudation. The fertilization rate of in-vitro matured oocytes was significantly lower than that of mature MII oocytes (52.7 and 70.8% respectively, P < 0.00l). Embryo quality was only slightly different as regards the numbers of good quality embryos: 47.4% good quality embryos were obtained in the in-vitro matured oocyte group, whereas 53.2% good quality embryos were obtained in the MII oocyte group (P < 0.05). The same proportions of excellent (5.7 and 7.0%, NS) and fair quality (17.6 and 15.3%, NS) embryos were obtained for in-vitro matured and mature oocytes respectively. Embryos derived from in-vitro matured oocytes were transferred only if they were of better quality or if there were not enough mature oocyte derived embryos available. Fifteen transfers involved only embryos derived from in-vitro matured oocytes: 11 single embryo transfers and four transfers of two embryos, resulting in one singleton pregnancy and the birth of a healthy baby. It may be concluded that in cycles with few MII oocytes it might be worthwhile to inject in-vitro matured MI oocytes in order to increase the number of embryos available for transfer. 相似文献
19.
The oxidizing agent tertiary butyl hydroperoxide induces disturbances in spindle organization, c-meiosis, and aneuploidy in mouse oocytes 总被引:3,自引:1,他引:3
Tarin Juan J.; Vendrell Francisco J.; Ten Jorge; Blanes Raquel; Van Blerkom Jonathan; Cano Antonio 《Molecular human reproduction》1996,2(12):895-901
It has been recently proposed that a concomitant generationof oxidative stress of oocytes with increasing maternal agemay be a major factor responsible for the age-related increasein aneuploid conceptions. As a preliminary step in the testingof this hypothesis, we need to confirm that oxidative stressin itself can induce errors in chromosome segregation. In orderto achieve this goal, germinal vesicle (GV)-stage mouse oocytesfrom unstimulated ICR and (C57BLxCBA) F1 hybrid female micewere matured in vitro for 9 h for metaphase I (MI) oocytes or16 h for metaphase II (MII) oocytes in the presence of varyingconcentrations of the oxidizing agent tertiary-butyl hydroperoxide(tBH). MII oocytes from (C57BLxCBA) F1 hybrid mice were fixedand C-banded for karyotyping analysis. MI and MII oocytes fromICR mice were fixed and stained with the DNAfluorescent probe4',6-diamidino-2-phenylindole (DAPI) to detect abnormalitiesin chromosomal distribution. Meiosis I and meiosis II spindlesfrom ICR mice were visualized by confocal immunofluorescencemicroscopy. Data from these experiments demonstrate that in-vitroexposure of mouse oocytes to tBH during meiosis I reduces thelength (pole-to-pole distance) and width (diameter at the equatorof the spindle) of meiosis I and meiosis II spindles. This reductionis associated with an increase in the percentage of oocytesshowing chromosome scattering and clumping on the MII plate,and of aneuploidy (hyperhaploidy) in MII oocytes. However, tBHat the concentrations used in the present study has only a minimalnegative effect on the frequency of meiotic maturation. Theseresults suggest that oxidative stress during meiotic maturationin vitro may induce chromosomal errors that are undetectablein the living oocyte and whose developmental consequences maybecome manifest after fertilization. aneuploidy/meiosis/mouse oxidative stress/spindle/tertiary butyl hydroperoxide 相似文献
20.
Seventy-six human oocytes lacking signs of fertilization 48h after insemination in vitro were fixed for chromosome analysis.Only one out of 61 oocytes showed mitotic chromosomes indicatingfertilization. Thus, non-cleavage is almost synonymous withnon-fertilization. Chromosomes in second meiosis were foundin 60 oocytes, of which 52 (86.7%) were analysable. Of these,only 18 (34.6%) displayed an apparently normal 23,X karyotype.Fifteen preparations showed no chromosomes at all but some containedsmall nuclei, most likely due to degeneration. An earlier stageof degeneration could be represented by a group of second meiosisoocytes (20.0%) carrying between 10 and 18 chromosomes. Thelarge proportion of chromosome abnormalities in this samplemight have been due to the fact that the oocytes were unfertilizedand perhaps that abnormal oocytes had matured due to ovarianstimulation. 相似文献