大豆总异黄酮的提取及含量测定

目的建立大豆总异黄酮的提取方法及含量测定方法。方法以大豆为原料通过乙醇浸泡提取,聚酰胺柱层析提取大豆总异黄酮。并用HPLC法测定大豆总异黄酮含量。结果提取物物大豆总异黄酮含量为52.26%。HPLC的条件为:C18柱,流动相为甲醇∶水∶乙酸=42∶57∶1,检测波长为260 nm。结论提取方法简单,测定方法简便、快捷,重现性好,可用于大豆总异黄酮的含量测定。     

大豆总异黄酮的提取及含量测定

目的建立大豆总异黄酮的提取方法及含量测定方法。方法以大豆为原料通过乙醇浸泡提取，聚酰胺柱层析提取大豆总异黄酮。并用HPLC法测定大豆总异黄酮含量。结果提取物物大豆总异黄酮含量为52．26％。HPLC的条件为：C18柱，流动相为甲醇：水：乙酸=42：57：1，检测波长为260nm。结论提取方法简单，测定方法简便、快捷，重现性好，可用于大豆总异黄酮的含量测定：     

大豆异黄酮苷元滴丸的制备和含量测定

目的:研究大豆异黄酮苷元滴丸的处方、制备工艺和含量测定方法.方法:用单因素实验法确定制备工艺中的各种条件;用正交设计法优选处方组成;用HPLC法测定异黄酮苷元的含量.结果:最佳工艺条件为:冷凝剂二甲基硅油温度10℃,药液温度80℃,滴距6 cm,滴速15滴/min.染料木素在3.6～36%、6.00%和0.89%.结论:本制备工艺稳定性好、成型率高,建立的含量测定方法可控性强、专属性高.     

正交设计法对大豆中提取异黄酮的工艺研究

目的探讨用乙醇提取大豆中异黄酮的工艺。方法大豆粉过40目筛,以石油醚脱脂后通过单因素实验和正交实验改变不同的提取工艺选取最佳提取条件。在70℃的条件下,用70%乙醇水溶液,以20∶1(v/w)的物料比,提取2次,时间分别为2 h和1.5 h。结果从大豆中所得粗异黄酮含量为0.4%,异黄酮的提取率可达92%。结论该方法可行,工艺可靠。     

大豆糖蜜中提取大豆异黄酮的研究

目的:研究一种从大豆糖蜜中简便提取大豆异黄酮的方法。方法:以大豆异黄酮含量和得率为考核指标,通过单因素试验研究最佳提取工艺。结果:固形物浓度20°,50℃下搅拌1h,3000rpm离心15min为最佳工艺条件,可制备含量20%以上的大豆异黄酮,应用于保健食品开发。     

淡豆豉不同制法其成品性状及异黄酮类化学成分含量的比较

目的:比较古法炮制与现今药典制法的淡豆豉其成品性状及异黄酮类化学成分的含量。方法:采用紫外-可见分光光度法对淡豆豉提取物中的总异黄酮进行含量测定;采用高效液相色谱法对淡豆豉提取物中异黄酮游离苷元(大豆素、染料木素)的含量进行测定。色谱柱为Prevail-C18(250 mm×10.0 mm,5μm),流动相为乙腈-水-乙酸(35∶64∶1,V/V/V),检测波长为260 nm,流速为1.0 ml/min,柱温为25℃,进样量为15μl。结果:古法炮制的淡豆豉气香,质地柔软,断面为棕褐色,表皮皱缩,总异黄酮含量为4.140 mg/g,大豆素含量为1.263 0 mg/g,染料木素含量为1.254 mg/g;现今药典制法的淡豆豉气味微弱,质地稍硬,断面为棕黄色,表皮皱缩,总异黄酮含量为3.530 mg/g,大豆素含量为0.349 mg/g,染料木素含量为0.335 mg/g。结论:古法炮制淡豆豉与现今药典制法淡豆豉相比,前者成品性状好、大豆异黄酮类含量高,古法炮制有明显优势。     

大豆异黄酮保健食品中苷及苷元的等度HPLC法测定

目的建立等度高效液相色谱法(HPLC)分离测定保健食品中大豆异黄酮三种苷及其苷元的方法.方法采用Inert-silODS-3(4.6 mmx150 mm,5 μm)色谱柱,以1.5%乙酸-甲醇-乙腈(7022.57.5)为流动相等度洗脱,检测波长260 nm,柱温55℃,流速1.2 mL·min-1.样品用70%的乙醇超声提取,经0.45 μm滤膜过滤后进样.结果HPLC过程于30 min左右完成,大豆苷和黄豆苷的分离度为0.9以上,余者分离度均大于1.5.对4种市售保健食品作了分析.结论方法简便、快速、准确,为含大豆异黄酮的保健食品含量测定及质量控制提供了较为理想的方法.     

高效液相色谱法测定中药品中大豆异黄酮不同组分

目的:建立采用高效液相色谱法(HPLC)检测中药品中大豆异黄酮4种组分的方法。方法:首先采用超声提取的方法进行处理样品,对提取溶剂进行了选择。色谱柱采用迪马C18柱,以甲醇和水为流动相进行梯度洗脱,检测波长为260 nm。结果:HPLC方法的回收率为88.8%～101.2%,RSD为2.1%～4.5%,检出限(以信噪比为3计)为0.5～0.8mg.L-1。结论:反相高效液相色谱方法简单,重现性好,能有效提取和分离大豆异黄酮中4种组分,值得进一步研究和分析。     

盐酸阿罗洛尔含量测定方法研究

目的：建立盐酸阿罗洛尔原料药的含量测定方法.方法：采用HPLC法和提取-滴定法两种方法测定盐酸阿罗洛尔原料的含量.结果：建立的HPLC含量测定方法准确、快速、简便,而提取-滴定法测定其含量存在一定问题.结论：在测定盐酸阿罗洛尔原料时,HPLC方法优于提取-滴定方法.     

微波及溶剂法提取豆粕中大豆异黄酮的工艺研究

目的探寻低温下获得较高提取率的大豆异黄酮提取方法。方法以低温脱脂豆粕为原料,用溶剂法和微波对豆粕进行提取,考察提取时间、提取温度、物料比、浓度等影响因素。利用紫外分光光度法测定大豆异黄酮的含量。结果用溶剂法提取大豆豆粕,在80%乙醇、常压60℃、浸提2h、豆粕与乙醇比例为1:15(g/mL)的条件下,大豆异黄酮的得率较高。利用微波对其进行前处理的最佳条件为频率400W,固液比为1:30(g/mL),微波提取时间为6min。结论溶剂法和微波法提取大豆异黄酮均可行,适当的微波处理不仅可提高大豆异黄酮的提取率,同时可提高提取操作的选择性,是一种有效的处理方法。     

Anti-inflammatory isoflavonoids from the stems of Derris scandens

Fractionation of the aqueous extract of Derris scandens stems extract using tests for eicosanoid inhibition resulted in the isolation of three isoflavonoids, genistein, its 7- O-alpha-rhamno(1-->6)-beta-glucosyl glycoside, a new compound, and two known isoprenyl derivatives 3'-gamma,gamma-dimethylallylweighteone and scandenin. The isoprenylated compounds showed a high inhibitory effect on eicosanoid production in vitro but HPLC analysis showed that the genistein accounted for most of the activity of the total extract. Antioxidant studies showed that genistein and the isoprenylated compounds showed activity comparable to standard antioxidants. Genistein and its glycoside demonstrated no cytotoxicity in the MTT test but the prenylated compounds showed some toxicity and also increased LDH release from polymorphonucleocytes, at concentrations much greater than would be encountered in an aqueous extract of D. scandens.     

Phytoestrogenic isoflavonoids in epidemiologic and clinical research

Isoflavones (IFLs) are natural products to which humans have been traditionally exposed predominantly through soy foods; more recently humans are also exposed to them through soy protein addition to processed foods or through supplements. They are structurally similar to steroidal estrogens and can exert estrogenic or antiestrogenic effects depending on their concentrations and on the tissue considered. These properties qualify IFLs to be classified as phytoestrogens and are believed to account for many of the biological effects observed for soy and/or IFL exposure including benefits for bone and heart health or prevention of menopausal symptoms and certain types of cancer. In order to evaluate the function of IFLs, alone or when exposure happens through soy intake, pharmacokinetics and bioavailability are critical issues to be considered in epidemiologic and clinical research. For this purpose precise, accurate, robust, fast, and affordable techniques for IFL analyses are required. Copyright © 2009 John Wiley & Sons, Ltd.     

射干异黄酮类化合物的分离与结构鉴定

目的研究中药射干[Belamcanda chinensis(L.)DC.]乙醇提取物中的化学成分。方法应用多种柱色谱方法进行分离和纯化,根据NMR和MS等波谱解析确定化合物结构。结果从射干根茎的乙醇提取物中分离出5个化合物,分别鉴定为:3′-羟基鸢尾苷(1)、鸢尾苷(2)、野鸢尾苷(3)i、ristectorin A(4)和异阿魏酸(5)。结论化合物1、5为首次从该属植物中分离得到,首次对化合物1的核磁信号进行归属。     

Erythrinan alkaloids and isoflavonoids from Erythrina poeppigiana.

A new erythrinan alkaloid, 8-oxo-alpha-erythroidine epoxide, was isolated from wood of Erythrina poeppigiana together with the five known compounds, 8-oxo-alpha-erythroidine, erystagallin C, alpinumisoflavone, erythrinin C and eryvarin A. Their structures were elucidated on the basis of spectroscopic evidence.     

Estrogenic activity of isoflavonoids from Onobrychis ebenoides

Fractionation of the neutral extract of Onobrychis ebenoides (Leguminosae) yielded a new isoflavone, named ebenosin (1), in addition to the known ones, afrormosin (2), formononetin (3) and daidzein (4). Although the relative binding affinities of 1 - 4 for estrogen receptor alpha (ERalpha) were nearly comparable and matched those of 1-3 for ERbeta, that of 4 for the latter receptor was significantly higher than any of the other. Compounds 1 - 4 induced cell proliferation and gene expression in breast and endometrial cancer cells in an ER-dependent manner. Nonetheless, the rank order of induction potencies ( 4 > 3 >or= 2 >or= 1) matched better that of affinities for ERbeta ( 4 > 3 >or= 2 >or= 1) rather than ERalpha ( 4 >or= 3 >or= 2 >or= 1). While the antiestrogen ICI 182,780 could inhibit the induction of proliferation of ER-positive breast cancer cells by 1-4, it could not prevent 1 from exhibiting significant ER-independent cytotoxicity at 10 microM. By contrast, 1 was much less cytotoxic and only weakly estrogenic for ER-positive endometrial adenocarcinoma cells. In conclusion, our data suggest that the C-8 isoprenyl substituent of 1 renders it cytotoxic and/or estrogenic in a cell-dependent manner.     

Neurobehavioral actions of coumestrol and related isoflavonoids in rodents

Isoflavonoids are plant estrogens that are increasingly advocated as a natural alternative to estrogen replacement therapy (ERT) and are available as dietary supplements. As weak estrogen agonists/antagonists with a range of other enzymatic activities, the isoflavonoids provide a useful model for the actions of endocrine disruptors. This paper reviews the responses of rodents to diets containing coumestrol or an isoflavone supplement in comparison to animals fed the phytoestrogen-free AIN76A diet. Neural mechanisms were investigated by examining isoflavonoid effects on ER(alpha)-dependent (regulation of oxytocin receptor [OTR] binding in the ventromedial nucleus of the hypothalamus [VMN]) and ERbeta-dependent (regulation of ERbeta mRNA in the paraventricular nucleus [PVN]) endpoints. Activational as well as organizational effects on sexual behavior and gonadotropin secretion were observed for coumestrol. Treatment of rat dams with a 100-ppm coumestrol diet from birth to postnatal day (PND) 21 induced premature anovulation in female offspring, and treatment from birth to PND 10 suppressed sexual behavior in male offspring. One-week treatment of ovariectomized (OVX) female rats with the same coumestrol diet increased ERbeta mRNA expression in the PVN, an effect opposite to that of estradiol. Ten days of treatment with a 200-ppm coumestrol diet increased LH secretion in OVX wild-type mice, an effect opposite to the normal negative feedback effects of estradiol. No effects were observed in ER(alpha) knockout (ER(alpha)KO)-OVX females, indicating that coumestrol's action on LH was mediated through ER(alpha). Similar activational effects were observed for the isoflavone diet. The lordotic response to estrogen was significantly reduced by 2 days of treatment of OVX adult females with an isoflavone diet providing 13 ppm genistein and 33 ppm daidzein. One week of treatment with the same isoflavone diet produced an effect opposite to that of estradiol in the PVN, increasing ERbeta mRNA expression above control levels. These investigations show that, in spite of their preferential affinity for ERbeta, isoflavonoids act through both ER(alpha) and ERbeta. Moreover, their neurobehavioral actions were antiestrogenic, either antagonizing or producing an action in opposition to that of estradiol. This work demonstrates that even small, physiologically relevant exposure levels can alter estrogen-dependent gene expression in the brain and complex behavior.     

Two new isoflavonoids from Erythrina variegata

Two new isoflavonoids, eryvarin A (1) and eryvarin B (2) were isolated from the wood of Erythrina variegata and their structures were elucidated on the basis of spectroscopic evidence.     

Anti-angiogenic and anti-tumor activities of isoflavonoids from the rhizomes of Belamcanda chinensis

The present study was carried out to clarify whether tectorigenin and tectoridin isolated from the rhizomes of Belamcanda chinensis (Iridaceae) inhibit angiogenesis by the experimental methods in vitro and in vivo. Tectorigenin and tectoridin decreased angiogenesis of both chick embryos in the chorioallantoic membrane assay and basic fibroblast growth factor-induced vessel formation in the mouse Matrigel plug assay. Both compounds also reduced the proliferation of calf pulmonary arterial endothelial (CPAE) cells and found to possess relatively weak gelatinase/collagenase inhibitory activity in vitro. Tectorigenin exhibited a much stronger anti-proliferative activity than its glycoside, tectoridin and was almost equipotent to that of genistein, a reference drug. Tectorigenin, when administered subcutaneously at the dose of 30 mg/kg for 20 days to mice implanted with murine Lewis lung carcinoma (LLC), caused a significant inhibition of tumor volume by 30.8 %. Tectorigenin and tectoridin, when treated i. p. at the same dosage for 10 days to ICR mice bearing sarcoma 180, caused a significant suppression in tumor weight by 44.2 and 24.8 %, respectively.