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1.
胸腺细胞对胸腺上皮细胞分泌IL—6的促进作用 总被引:4,自引:0,他引:4
为分析胸腺细胞对胸腺基质细胞功能的调节作用,将胸腺细胞与小鼠胸腺上皮细胞系(MTEC1)共育,分析胸腺细胞对MTEC1分泌IL-6的影响。结果表明,胸腺细胞能明显促进MTEC1分泌IL-6,其促进程度与两种细胞的数量及共育时间有关。胸腺细胞(4×10^6/孔)与MTEC1(1×10^5/孔)共育48小时,MTEC1分泌IL-6达高峰。去除胸腺细胞后96小时,MTEC1分泌IL-6的量仍比单独培养的 相似文献
2.
目的研究胸腺细胞和ICAM-1抗体对胸腺上皮细胞分泌IL-6的作用。方法用促IL-6依赖株增殖法检测IL-6活性,用原位杂交法检测IL-6mRNA的表达。结果不同的胸腺细胞亚群均能促进MTEC1分泌IL-6,而且作用无明显差异。ICAM-1抗体能够促进MTEC1分泌IL-6,其促进作用呈剂量依赖关系。放线菌酮预处理MTEC1以后,ICAM-1抗体和胸腺细胞均不再能促进MTEC1分泌IL-6。原位杂交证实,经胸腺细胞和ICAM-1抗体活化后,MTEC1表达IL-6mRNA明显增多,并且表达IL-6mRNA的MTEC1数量也增多。提示胸腺细胞和ICAM-1抗体能促进MTEC1合成新的蛋白和mR-NA。结论胸腺微环境内,胸腺细胞可以通过粘附分子与胸腺上皮细胞相互作用,并可促进胸腺上皮细胞分泌IL-6。 相似文献
3.
孙永莲 《国外医学:免疫学分册》1994,17(6):285-289
人胸腺上皮细胞(TEC)的体外培养和传代。使研究人TEC的特性和功能成为可能。人TEC体外培养成功的关键是抑制成纤维细胞的生长。体外培养的TEC除表达角蛋外,还表达HLA-I类抗原,LFA-3,ICAM-1和CD40等表面抗原;可分泌IL-1,IL-6,IL-8,GM_CSF,G_CSF和M_CSF等多种细胞因子和胸腺素;静息的胸腺细胞与TEC的结合由CD2/LFA-3介导,激活的胸腺细胞与TEC 相似文献
4.
目的分析胸腺细胞对胸腺上皮细胞分泌干扰素的影响。方法用胸腺细胞和胸腺基质细胞混合培养模型,测定IFN活性并进行类型鉴别。结果胸腺细胞可以促进小鼠胸腺上皮细胞系(MTEC1)产生Ⅰ型IFN,胸腺细胞和胸腺基质细胞以401的比例培养时,促进作用最强。MTEC1细胞经抗ICAM-1抗体作用后,分泌Ⅰ型IFN增多。用放线菌酮预处理MTEC1细胞后,胸腺细胞和抗ICAM-1抗体则不再影响MTEC1细胞产生IFN。结论胸腺细胞与基质细胞的相互作用,可由粘附分子ICAM-1/LFA-1介导。胸腺细胞能促进MTEC1细胞合成IFN。 相似文献
5.
胸腺细胞对胸腺基质细胞生长及功能的调节 总被引:1,自引:0,他引:1
目的研究胸腺细胞对不同亚群胸腺基质细胞生长及功能的调节作用。方法胸腺基质细胞增殖以3H-TdR掺入法测定,IL-7活性以促IL-7依赖株法检测,其mRNA表达以RT-PCR法检测。结果胸腺细胞与MTSC4细胞分别以80∶1,40∶1或20∶1的比例共育时,能明显抑制MTSC4细胞的增殖,而培养上清中IL-7活性无明显改变;当两种细胞的比例为10∶1或5∶1时,则对MTSC4细胞的增殖无任何影响,而培养上清中的IL-7活性明显升高。RT-PCR证实,与胸腺细胞共育的MTEC1和MTSC4细胞,IL-7mRNA水平明显提高。结论胸腺细胞能促进MTEC1和MTSC4细胞分泌IL-7,对不同亚群基质细胞的生长和功能有不同调节作用 相似文献
6.
用本室建立的小鼠胸腺上皮细胞株MTECB,分别在1.无钙镁Hank液中(4℃);2.无钙镁Hank液中(37℃);3.含钙镁Hank液中(4℃)下;4.含钙镁Hank液中(37℃)与胸腺细胞共育(基质细胞:胸腺细胞=1:10),光镜和扫描电镜下观察它们之间的结合特点。发现在各种实验条件下,90%的MTECB能与胸腺细胞结合,这种结合从形态上分为两种形式,其一为胸腺细胞均匀结合于MTECB周围,呈花 相似文献
7.
MTEC 1分泌的趋化因子引起特定亚群胸腺细胞的定向迁移 总被引:9,自引:0,他引:9
分析胸腺髓质上皮样细胞系MTEC1分泌的化学趋化因子对胸腺细胞亚群的趋化作用。方法以抗体加补体杀伤结合免疫磁珠及panning法,将小鼠胸腺细胞分离纯化,获得CD4+CD8+(DP),CD4-CD8-(DN),CD4+CD8-(CD4SP)及CD4-CD8+(CD8SP)四亚群细胞,用Boyden小室分析MTEC1┐SN对四群胸腺细胞的趋化作用。结果MTEC1┐SN对DP及CD4SP胸腺细胞有趋化活性(CI=6.6±1.0及6.1±1.8);对CD8SP细胞有中度趋化活性(CI=3.2±1.0);对DN趋化活性微弱(CI=1.3±0.6)。化学趋化因子MCP┐1纯品对CD4SP胸腺细胞显示强趋化活性(CI=5.6),对DN胸腺细胞则无可测出趋化活性。结论MTEC1分泌的化学趋化因子对DP,CD4SP及CD8SP胸腺细胞有显著趋化作用,对DN胸腺细胞几乎无趋化作用。提示此类化学趋化因子有趋使胸腺发育中后期阶段的细胞向胸腺髓质区迁移和定位的作用。 相似文献
8.
两种小鼠胸腺基质细胞对胸腺细胞凋亡的不同作用 总被引:4,自引:2,他引:4
采用两种体外建系的小鼠胸腺基质细胞(TSC)系,即上皮样TSC(MTEC1)和树突状TSC(MTSC4),观察其对胸腺细胞凋亡的影响。小鼠胸腺细胞在体外培养过程中,可自发地出现细胞凋亡的特征,表现为DNA呈梯度断裂片段,细胞经FACS分析出现亚二倍体DNA波峰,以及Feulgen′s染色镜检所见的DNA凝聚和断裂。胸腺细胞在与TSC共育后,在MTEC1组可见其凋亡过程受到抑制和存活率的增加;在MTSC4组,仅在共育12至18小时时,见到胸腺细胞凋亡加强,而其存活率不受影响。结果提示在胸腺细胞发育过程中,其阴性选择作用的主要机制之一的PCD过程受不同来源的胸腺基质细胞的调节。 相似文献
9.
人胸腺上皮细胞(TEc)的体外培养和传代,使研究人TEc的特性和功能成为可能。人TEc体外培养成功的关键是抑制成纤维细胞的生长。体外培养的人TEc除表达角蛋白外,还表达HLA-I类抗原、LFA-3,ICAM-1和CD40等表面抗原;可分泌IL-1,IL-6,IL-8,GM-CSF,G-CSF和M-CSF等多种细胞因子和胸腺素;静息的胸腺细胞与TES的结合由CD2/LFA-3介导,激活的胸腺细胞与TEC的结合由CD2/LFA-3和LFA-1/ICAM-1共同介导;TEC对成熟的胸腺细胞有辅助增殖的作用,对不成熟的胸腺细胞有直接激活作用:胸腺细胞可通过直接作用和分泌细胞因子的间接作用来调节TEC的细胞因子分泌和表面抗原表达。 相似文献
10.
为了分析胸腺细胞在胸腺上皮细胞发育中的作用,本文作者在体外分析了胸腺细胞对小鼠胸腺上皮细胞系(MTEC1)增殖的作用。结果表明,不同的胸腺细胞亚群以及胸腺细胞裂解液均能促进MTEC1增殖,考的松耐受性胸腺细胞和CD3-4-8-细胞具有同样的促进MTEC1增殖的作用,其促进程度与两种细胞的数量有关,胸腺细胞和MTEC1为201或401时,其促进作用最强。胸腺细胞裂解液在高浓度(12稀释)时,能促进MTEC1增殖,在低浓度时则不影响MTEC1的增殖。本实验提示,胸腺细胞可以通过细胞-细胞相互作用以及分泌细胞因子影响MTEC1的增殖。 相似文献
11.
Over 200 schizophrenic patients belonging to three major and interrelated pedigree complexes have been investigated over the past 30 years in a North Swedish geographically isolated population, presently numbering about 6,000. An intensive investigation of a number of biochemical correlates and genetic markers in a few selected families belonging to one of the major pedigrees has indicated new strategies for the current research program.
Schizophrenia, as defined operationally, is significantly associated with decreased activities of two enzymes (1) blood platelet monoamine oxidase, (2) plasma dopamine-β-hydroxylase, and (3) with the genetic marker Gc2 (group specific antigen). Both enzymes are subject to genetic variation. A positive score for linkage between schizophrenia and low plasma DBH activity has been calculated, but, so far, available data are insufficient for discrimination between linkage and partial contribution of genetically controlled low plasma DBH to the pathogenesis of the disease. Alternatively, both mechanisms could be involved.
As a model for continued research, schizophrenia is explained as based on a double dominant-recessive genotype (Aabb), representing a vulnerability which in about 50 % of cases develops into clinical schizophrenia. It is suggested that the dominant mutation (A) operates on or affects MAO activity, and that the recessive genotype (bb) is instrumental in low variates of DBH activity and very likely such variates within the normal range of physiological variation. Moreover, it is suggested that the combined effects of MAO- and DBH-reduced efficiency on the metabolism of e.g. dopamine could be an essential pathogenic mechanism for the schizophrenic illness which is segregating in this population. 相似文献
Schizophrenia, as defined operationally, is significantly associated with decreased activities of two enzymes (1) blood platelet monoamine oxidase, (2) plasma dopamine-β-hydroxylase, and (3) with the genetic marker Gc
As a model for continued research, schizophrenia is explained as based on a double dominant-recessive genotype (Aabb), representing a vulnerability which in about 50 % of cases develops into clinical schizophrenia. It is suggested that the dominant mutation (A) operates on or affects MAO activity, and that the recessive genotype (bb) is instrumental in low variates of DBH activity and very likely such variates within the normal range of physiological variation. Moreover, it is suggested that the combined effects of MAO- and DBH-reduced efficiency on the metabolism of e.g. dopamine could be an essential pathogenic mechanism for the schizophrenic illness which is segregating in this population. 相似文献
12.
Renal dysplasia and asplenia in two sibs 总被引:2,自引:0,他引:2
A family is reported in which two sibs, one male and the other female, both died within 24 hours of birth with enlarged polycystic kidneys. Postmortem histology in the second child showed gross renal dysplasia. In both children the pancreas was enlarged, nodular and cystic but the liver appeared macroscopically normal. In the second child, histological examination confirmed pancreatic fibrosis with cystic dilation of ducts, but showed portal fibrosis with bile duct proliferation in the liver.
This combination of findings is very reminiscent of those in a girl and her brother reported by Ivemark et al. (1959). The children reported here also showed absence or hypoplasia of the spleen, cardiac anomalies and other features of the Ivemark syndrome (Ivemark 1955), a quite different, usually sporadic, congenital disorder. It is suggested that the children described here have a distinct lethal congenital disorder, probably inherited in an autosomal recessive manner. 相似文献
This combination of findings is very reminiscent of those in a girl and her brother reported by Ivemark et al. (1959). The children reported here also showed absence or hypoplasia of the spleen, cardiac anomalies and other features of the Ivemark syndrome (Ivemark 1955), a quite different, usually sporadic, congenital disorder. It is suggested that the children described here have a distinct lethal congenital disorder, probably inherited in an autosomal recessive manner. 相似文献
13.
Donald O. Rudin 《Medical hypotheses》1982,8(1):17-47
About 1900, modern food selection and processing caused widespread of the B vitamin deficiency diseases of beriberi and pellagra which, for genetic reasons, often expressed as different diseases ranging from bowel and heart disease to dermatoses and psychoses. But the B vitamins merely help convert essential fatty acids (EFA) into the prostaglandin (PG) tissue regulators and it now turns out that, through hydrogenation, milling and selection of w3-poor southern foods, we have also been systematically depleting, by as much as 90%, a newly discovered trace Nordic EFA (w3) of special importance to primates and sole precursor of the PG3(4) series, even as a concurrent fiber deficiency increases body demand for EFA. Since substrate EFA is processed by many B vitamin catalysts, an EFA deficiency will mimic a ypovitaminosis B, i.e., a mixture of beriberi and pellagra resembling vitamin beriberi and pellagra but exhibiting as even more diverse disease. This would consitute a second stage of the Modern Malnutrition and explain why some workers now hold the dominant diseases of modermized societies to be new, nutritionally based, pellagraform yet lipid-related and to range, once again, from heart disease to psychosis. It is an assumption that our dominant diseases are unrelated to each other or are merely revealed by our diagnostic acumen and therapeutic success; and that hydrogenating millions of tons of food oils annually, to destroy the rancidity producing w3-EFA, is safe for . Extensive beriberiform disease is reported here in 32 typical cases taken from medical practice which responds strikingly to linseed oil supplements (60% w3-EFA) in confirmation of identical results in Capuchins. 相似文献
14.
15.
Newton H 《Medical history》2011,55(2):153-182
Sick children were ubiquitous in early modern England, and yet they have received very little attention from historians. Taking the elusive perspective of the child, this article explores the physical, emotional, and spiritual experience of illness in England between approximately 1580 and 1720. What was it like being ill and suffering pain? How did the young respond emotionally to the anticipation of death? It is argued that children’s experiences were characterised by profound ambivalence: illness could be terrifying and distressing, but also a source of emotional and spiritual fulfilment and joy. This interpretation challenges the common assumption amongst medical historians that the experiences of early modern patients were utterly miserable. It also sheds light on children’s emotional feelings for their parents, a subject often overlooked in the historiography of childhood. The primary sources used in this article include diaries, autobiographies, letters, the biographies of pious children, printed possession cases, doctors’ casebooks, and theological treatises concerning the afterlife. 相似文献
16.
C. R. Lipton J. X. Dautlick G. D. Grothaus P. L. Hunst K. M. Magin C. A. Mihaliak F. M. Rubio J. W. Stave 《Food and Agricultural Immunology》2000,12(2):153-164
Recent advancements in agricultural biotechnology have created a need for analytical techniques to determine introduced proteins in crops enhanced through modern biotechnology techniques. These proteins are expressed in plant tissues and may be present in food ingredients. Immunoassays are ideally suited for protein detection and may be used as both quantitative and threshold methods. Microplate ELISA and lateral flow devices are two of the most commonly used immunoassay formats for agricultural biotechnology applications. This paper provides general background information and a discussion of criteria for the validation and application of immunochemical methods to the analysis of proteins introduced into plants and food ingredients using biotechnology methods. It is the result of a collaborative effort of members of the Analytical Environmental Immunochemical Consortium. This collaborative effort represents the combined expertise of several organizations to reach consensus on establishing guidelines for the validation and use of immunoassays. Further, the paper offers developers and users a consistent approach to adopting the technology as well as aid in producing accurate and meaningful results. 相似文献
17.
Antonio Arnaiz-Villena Jose Palacio-Grüber Ignacio Juarez Ennio Hernández Ester Muñiz Brayan Bayona Cristina Campos Jorge Nieto Manuel Martin-Villa Carlos Silvera 《Human immunology》2018,79(4):189-190
HLA-A,-B,-C,-DRB1 and -DQB1 alleles have been studied in Chimila Amerindians from Sabana de San Angel (North Colombian Coast) by using high resolution molecular typing. A frequent extended haplotype was found:HLA-A*24:02-B*51:10-C*15:02-BRB1*04:07-DQB1*03:02 (28.7%) which has also been described in Amerinndian Mayos Mexican population (Mexico, California Gulf, Pacific Ocean). Other haplotypes had already been found in Amerindians from Mexico (Pacific and Atlantic Coast), Peru (highlands and Amazon Basin), Bolivia and North USA. A geographic pattern according to HLA allele or haplotype frequencies is lacking in Amerindians, as already known. Also, five new extended haplotypes were found in Chimila Amerindians. Their HLA-A*24:02 high frequencies characteristic is shared with aboriginal populations of Taiwan; also, HLA-C*01:02 high frequencies are found in New Zealand Maoris, New Caledonians and Kimberly Aborigines from Australia. Finally, this study may show a model of evolutionary factors acting and rising one HLA allele frequency (-A*24:02), but not in others that belong to the same or different HLA loci. 相似文献
18.
Ultracryotomy: development and application (with examples from the yeast cytology) (author's transl)
Konrad Joachim Böhm 《Acta histochemica》1980,66(1):59-84
The preparation steps usually necessary for obtaining ultrathin frozen sections of biological material (chemical prefixation, enclosing, cryoprotective treatment, freezing, sectioning, and post-staining the sections for transmission electron microscopy) are submitted to a critical analysis. The application of cryo-ultramicrotomy, in particularly for cytochemical purposes, is reviewed. Fundamental considerations of chemical prefixation and poststaining are supported by examples from yeast cytology. Furthermore, the efficiency of the cryo-ultramicrotomy (electron optical resolution of ultrastructural details) is demonstrated on yeast cells and protoplasts. 相似文献
19.
M G Baggot 《Medical hypotheses》1979,5(5):591-597
Starting with the integument, we see many organs are contractile sacs or multiples thereof, which tubes or bags constitute the major part of the entire body. Recognition of this basic unit and its characteristics sheds new light, individually and collectively, on many disorders previously considered unrelated. Muscular tears and perforations develop in the walls of these chambers, being no way peculiar to those organs, wherein, hydrochloric acid occurs. So, it is not necessary to explain the absence of excessive acid from patients who exhibit holes in the gastric, uterine, aortic, duodenal, rectal, pulmonary, retina, and other walls. Muscle, not acid is the great common factor relating idiopathic disorders in the gastrointestinal tract to each other and to similar diseases in other systems. When the units are linked together, the lesions tend to appear as arthropathies, i.e. at the joints. Rephrasing common-place observations, frees us from conventional, conceptual cul-de-sacs. An observation is only as good as its interpretation, so all possibilities must be considered, otherwise, we will remain blinded by our misconceptions. 相似文献
20.
G. Heinemann H. Schievelbein D. Eberhagen V. Rahlfs 《Journal of molecular medicine (Berlin, Germany)》1981,59(20):1165-1173
Zusammenfassung Der Einfluß von verschiedenen Nahrungsmitteln auf Methoden zur Bestimmung von Adrenalin (AD), Noradrenalin (NA), Vanillinmandelsäure (VMS), Metanephrinen (MN), Homovanillinsäure (HVS) und 5-Hydroxyindolessigsäure (5-HIE) im 24 h-Harn zur Diagnose des Phäochromozytoms bzw. Karzinoid-Syndroms wurde untersucht. Die in die Untersuchung einbezogenen Nahrungsmittel waren: Tee, Kaffee, Mandeln, Ananas, Käse, Walnüsse, Vanillepudding, Bananen, Tomaten und Milchschokolade. Außerdem wurde der Einfluß des Zigarettenrauchens auf die Bestimmung von AD, NA, VMS und MN untersucht.Walnüsse führten zu einer starken Erhöhung der 5-HIE-Ausscheidung. Bananen erhöhten die Ausscheidung von AD, NA, VMS, MN und 5-HIE. Kaffee und Ananas bewirkten eine geringe Zunahme der MN-Werte. Rauchen von 20–30 Zigaretten/Tag beeinflußte keine der vier Variablen.Wenn die beschriebenen Methoden benutzt werden, sollte lediglich auf den Verzehr von Bananen und Walnüssen vor und während der Harnsammelperioden verzichtet werden, da die oberen Normgrenzen im Harn überschritten werden könnten. Ein Verzicht auf Kaffee und Ananas in normalen Mengen ist nicht erforderlich. Es besteht kein Anlaß, weiterhin die bisherigen umfangreichen Restriktionen der übrigen Nahrungsmittel beizubehalten. 相似文献