心理一致感量表(SOC-13)的信、效度初步研究

目的:对Antonovsky编制的心理一致感量表(SOC-13)进行初步的修订和信效度检验。方法: 根据量表修订的方法,在上海市选取1827名成年被试和44名大学生被试进行测试,分析中文版的信、效度。结果:项目分析结果表明SOC-13各项目的项目鉴别力良好r=0．40、0．64,有三个组的r在0．28- 0．37;量表的重测信度为0．61,内部一致性系数为0．76;探索性因素分析的结果得到可理解感、可控制感和意义感三个因素(特征根大于1,解释方差比率总计47％),除项目9和11外,其他条目的负荷范围在 0．41至0．69之间;效标关联效度理想。结论:修订的心理一致感量表具有较高的信、效度。     

心理一致感利兹堡简短版(SOC-L9)中文版的信效度检验

目的 对心理一致感利兹堡简短版(SOC-L9)中文版进行初步的修订和信效度检验.方法 根据量表修订的方法,在某大学选取3349名在校大学生和研究生被试进行测试,分析中文版的信度和效度.结果 项目分析结果表明SOC-L9各项目的项目鉴别力良好(r=0.315～0.698);内部一致性系数为0.732,分半信度0.757,重测信度为0.621;效标关联效度理想.结论 心理一致感利兹堡简短版(SOC-L9)的中文版在大学生中具有较高的信效度.     

中文版Utrecht工作投入量表(UWES)的信效度检验

目的：引入Schaufeli等编制的Utrecht工作投入量表（U8WES），检验其在中学教师群体中应用的信度和效度。方法：使用UWES中译本，对47名中学教师进行初测，删去2个鉴别度过低的项目。使用中文版Utrecht工作投入量表、SF-36健康调查量表、COPE应对量表和中学教师工作特征问卷对277名中学教师进行正式测量，检验各项信、效度指标。结果：验证性因素分析结果验证了工作投入的三因素模型，各分量表内部一致性信度良好。教师的工作投入与总体健康状况、应对策略、工作特征等效标之间存在预期的相关。结论：修订后的中文版UWES信、效度较高，可为国内相关研究所采用。     

心理一致感的潜结构研究

目的：探讨Antonovsky开发的13个项日的心理一致感量表在中国大陆的适用性。方法：采用实证研究方式对656名被试的样本数据进行了探索性及验证性因索分析，结果：个体的心理一致感是由可理解性、易处理性及意义性三个成分构成的总体．支持Antonovsky提出的关于心理一致感的要念构思。结论：心理一致感量表对中国大陆样本基本上是适用的，但个别题目有待进一步修正。     

施瓦茨价值观问卷(PVQ-21)中文版在大学生中的修订

目的:对施瓦茨价值观问卷(PVQ-21)中文版在在校大学生和研究生中进行修订。方法:根据量表修订的方法,在某大学选取3351名在校大学生和研究生被试进行测试,分析中文版的信度和效度。结果:项目分析结果表明,PVQ-21各项目的项目鉴别力良好(r=0.753~0.907);内部一致性系数为0.337~0.742,分半信度为0.337~0.746,重测信度0.151~0.669;PVQ-21的最小空间分析结果基本符合理论模型;PVQ-21与心理一致感利兹堡简短版(SOC-L9)和Connor-Davidson韧性量表(CD-RISC)显著相关,效标关联效度理想。结论:施瓦茨价值观量表(PVQ-21)中文版在大学生中具有较高的信效度。     

Wagnild-Young心理弹性量表(RS-11)中文版的信效度检验

目的 对Wagnild-Young心理弹性量表(RS-11)进行初步的修订和信效度检验.方法 根据量表修订的方法,选取某大学在校生3363名进行测试,分析中文版的信、效度.结果 项目分析结果表明RS-11各项目的项目鉴别力良好(r=0.344～0.700);内部一致性系数为0.807,分半信度为0.865,量表的重测信度为0.617;与CD-RISC-25关联效度为0.725;与大五人格和SCL-90关联效度理想.结论 修订的Wagnild-Young心理弹性量表(RS-11)具有较高的信度和效度.     

Preliminary Revision of Perceived Autonomy Support Scale for Junior Middle School Students

目的 修订由Williams和Deci编制的学生对教师的自主支持感量表-学习气氛问卷(The Learning Climate Questionnaire,LCQ).方法 用修订的自主支持感量表对安徽省2所中学464名初中生进行测试,并对修订量表的信、效度进行分析.结果 修订量表中的各项目有较好的区分度;探索性因素分析结果显示,修订的自主支持感量表是单维的,这与原量表结构一致,单因素潜变量可解释总方差的43.73％;CFA对此结构模型进行了验证,其中x2/df=2.74,GFI=0.88,AGFI=0.83,NFI=0.92,RMSEA=0.074,CFI=0.95,各项拟合指数均符合测量学要求;另外,修订量表的内部一致性系数这0.89,分半信度为0.81,重测信度为0.73.结论 修订版的自主支持感量表具有较好的信度和效度,可用于初中生积极心理的进一步研究.     

汉区少数民族学生文化疏离感的理论建构及量表编制

目的：编制《汉区少数民族学生文化疏离感》测量量表，并检验该量表的信、效度。方法：提出文化疏离感的操作性定义。通过对汉区少数民族学生进行开放式问卷调查和访谈收集问卷项目，然后进行小样本初步测查、修订问卷、大样本测查，最后确定了《汉区少数民族学生文化疏离感》的测量量表。结果：该量表的CRONBACH(α)系数、分半信度、题项与总分的相关指标较好；通过探索性因素分析和验证性因素分析构想出文化疏离感的四个维度：文化孤立感、文化分离感、不和谐感、被控感。结论：《汉区少数民族学生文化疏离感》量表具有较好的信度和效度指标，具有一定理论意义。     

自主支持感量表在初中生中的初步修订

目的修订由W illiam s和D eci编制的学生对教师的自主支持感量表—学习气氛问卷(The L earn ing C lim ateQ uestionna ire,LCQ)。方法用修订的自主支持感量表对安徽省2所中学464名初中生进行测试,并对修订量表的信、效度进行分析。结果修订量表中的各项目有较好的区分度;探索性因素分析结果显示,修订的自主支持感量表是单维的,这与原量表结构一致,单因素潜变量可解释总方差的43.73%;CFA对此结构模型进行了验证,其中χ2/d f=2.74,GF I=0.88,AGF I=0.83,NF I=0.92,RM SEA=0.074,CF I=0.95,各项拟合指数均符合测量学要求;另外,修订量表的内部一致性系数达0.89,分半信度为0.81,重测信度为0.73。结论修订版的自主支持感量表具有较好的信度和效度,可用于初中生积极心理的进一步研究。     

儿童对婚姻冲突的感知量表修订

目的：由Grych等人编制的儿童对婚姻冲突的感知量表(CPIC)在国外已经被广泛使用，本研究是对该量表进行修订并考察其心理测量学特征，确定其是否适用于中国儿童。方法：采用CPIC量表对303名2—6年级儿童进行了测试并做信、效度检验。结果：因素分析和聚类分析结果表明，修订后的CPIC量表具有较高的构想效度数值；量表的内部一致性信度为0．879，分半信度为0．865，各维度的a系数均在0．657以上，具有较高的信度。结论：CPIC量表可以用于中国儿童的有关研究。     

Studies on the structure and function of the apolipoprotein(a) gene

Lp(a) is an LDL-like lipoprotein that is a major inherited risk factor for atherosclerosis. It is distinguished from Lp(a) by the addition of apolipoprotein(a). The gene structure of apolipoprotein(a) is homologous to plasminogen, and competition with plasminogen activity may account for some of the pathophysiology associated with Lp(a). Six highly related genes have now been identified, and at least four are found in close proximity in overlapping genomic clones. Studies have begun on the regulation of apolipoprotein (a) gene expression, and the human apolipoprotein(a) gene has been inserted into transgenic mice, where it leads to the development of arterial lesions.     

Vacuum Ultraviolet Photolysis of Hydrocarbon Polymers

Summary: The wavelength‐dependent vacuum ultraviolet (VUV) photolysis of several polymers, low density polyethylene (LDPE), biaxially oriented poly(propylene) (BOPP), atactic polystyrene (PS), and poly(methyl methacrylate) (PMMA), was studied by irradiation in vacuum with the well‐characterized emissions from four different resonant or excimer VUV sources. These lamps comprise radiofrequency (r.f.) discharges in different noble gases, such as krypton, xenon (at low pressures, producing near‐monochromatic resonant line radiations), xenon excimer (at “high” pressure), and a deuterium/argon mixture (producing a broad‐band emission). VUV‐induced mass loss (ablation or etching) was monitored in situ by quartz crystal microbalance measurements. Following irradiation, samples were analysed by ATR‐FTIR and XPS, to evaluate near‐surface structural changes (e.g., creation of unsaturation, cross‐linking) resulting from the VUV‐initiated bond scissions and radical‐creation reactions. PMMA was the most readily ablatable polymer, whereas the mass loss of BOPP was higher than that of LDPE, regardless of the irradiation wavelength, λ. All polymers were found to form double bonds, with the exception of PS, which is rather stable, probably due to energy dissipation by fluorescence.

Formation of double bonds in a) vinyl‐, b) vinylidene‐, and c) vinylene‐like unsaturated groups, as a function of the radiation dose, D, for KrL (?), XeL (?), and D₂Ar‐irradiated (?) PMMA.     

新生儿缺氧缺血性脑病患者血浆ET、SS测定的临床意义

目的:探讨了新生儿缺氧缺血性脑病患者血浆内皮素(ET)和生长抑素(SS)水平的变化及临床意义.方法:应用放射免疫分析对63例新生儿缺氧缺血性脑病患者进行了血浆ET和SS检测,并与35名正常健康新生儿作比较.结果:病儿组血浆ET水平显著高于正常儿组,而SS水平则显著低于正常儿组(P＜0.01).结论:新生儿缺氧缺血性脑病的发生发展与ET和SS水平的变化密切相关.     

Distribution of six neuropeptides in the nucleus tractus solitarii of the rat: an immunohistochemical analysis

Distribution of substance P-, [Leu]enkephalin-, cholecystokinin-8-, neurotensin-, avian pancreatic polypeptide- and gamma-melanocyte stimulating hormone-like immunoreactive structures were investigated in the nucleus tractus solitarii of the rat by means of the indirect immunofluorescence method. The density of the immunoreactive structures varied markedly according to neuropeptides or subnuclei, with the medial and commissural nuclei containing the highest density. This suggests that the peptides examined play a role in cardiovascular function. However, as seen in the substance P- and [Leu]enkephalin-like immunoreactive structures, these peptides were widely distributed in the nucleus tractus solitarii in addition to the commissural and medial nuclei; a high density of immunoreactive fibers in the ventral, dorsolateral and intermediate subnuclei. In addition to the immunoreactive fiber plexus, a group of immunoreactive cells was also identified in the subnuclei mentioned above. These findings strongly suggest that substance P- and [Leu]enkephalin-like immunoreactive structures are involved not only in cardiovascular function but also in other functions such as respiration, at least in the rat. Finally, the present study demonstrated that the area postrema, particularly its lateral portion, contains various neuropeptide-like structures, both neurons and fibers, substance P-, [Leu]enkephalin-, cholecystokinin-8- and neurotensin-like immunoreactive neurons and fibers, and avian pancreatic polypeptide- and gamma-melanocyte stimulating hormone-like immunoreactive fibers.     

脑血栓形成患者LPA、MDA、SOD检测的临床分析

目的:探讨溶血磷脂酸(LPA)和氧自由基在脑血栓形成的病理过程中的作用.方法:通过检测90例脑血栓形成患者(CT组)血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、LPA的浓度情况,并与短暂性脑缺血发作(TIA)组(45例)、对照组(45例)比较.结果:血清中SOD的浓度,CT组(治疗前)低于TIA组和对照组(P<0...     

聚羟基烷酸酯、聚丁二酸丁二酯及其共混材料的细胞相容性评价比较

取家兔的骨髓间充质干细胞(MSCs)分别种植到聚羟基烷酸酯(PHBV)、聚丁二酸丁二酯(PBS)、及其二者共混材料(PHBV/PBS)上,每组材料又分为有孔和实心两种构型。用MTT实验、乳酸脱氢酶(LDH)实验、甲苯胺蓝染色和电镜等方法,观察MSCs在材料上的生长情况。比较三种材料六种构型分别对MSCs黏附和增殖的影响。结果以共混物实心材料的毒性最低,细胞增殖率最高。甲苯胺蓝染色结果显示兔MSCs在共混实心材料上的生长最佳,扫描电镜结果显示兔MSCs与共混实心材料黏附良好。共混物实心材料很有希望成为制备组织工程血管新型的支架材料。     

The regulation of non-coding RNA expression in the liver of mice fed DDC

Mallory-Denk bodies (MDBs) are found in the liver of patients with alcoholic and chronic nonalcoholic liver disease, and hepatocellular carcinoma (HCC). Diethyl 1,4-dihydro-2,4,6,-trimethyl-3,5-pyridinedicarboxylate (DDC) is used as a model to induce the formation of MDBs in mouse liver. Previous studies in this laboratory showed that DDC induced epigenetic modifications in DNA and histones. The combination of these modifications changes the phenotype of the MDB forming hepatocytes, as indicated by the marker FAT10. These epigenetic modifications are partially prevented by adding to the diet S-adenosylmethionine (SAMe) or betaine, both methyl donors. The expression of three imprinted ncRNA genes was found to change in MDB forming hepatocytes, which is the subject of this report. NcRNA expression was quantitated by real-time PCR and RNA FISH in liver sections. Microarray analysis showed that the expression of three ncRNAs was regulated by DDC: up regulation of H19, antisense Igf2r (AIR), and down regulation of GTL2 (also called MEG3). S-adenosylmethionine (SAMe) feeding prevented these changes. Betaine, another methyl group donor, prevented only H19 and AIR up regulation induced by DDC, on microarrays. The results of the SAMe and betaine groups were confirmed by real-time PCR, except for AIR expression. After 1 month of drug withdrawal, the expression of the three ncRNAs tended toward control levels of expression. Liver tumors that developed also showed up regulation of H19 and AIR. The RNA FISH approach showed that the MDB forming cells' phenotype changed the level of expression of AIR, H19 and GTL2, compared to the surrounding cells. Furthermore, over expression of H19 and AIR was demonstrated in tumors formed in mice withdrawn for 9 months. The dysregulation of ncRNA in MDB forming liver cells has been observed for the first time in drug-primed mice associated with liver preneoplastic foci and tumors.     

血清C肽浓度测定的临床意义探讨

目的： 研究血清C肽浓度及胰岛素与C肽比值的临床意义。方法：用放射免疫分析测定2型糖尿病和肾功能不全患者血清C肽、胰岛素浓度,并计算胰岛素与C肽比值。结果：肾功能不全组病人血清C肽、胰岛素水平显著高于糖尿病各组,且胰岛素与C肽比值显著低于糖尿病各组（P〈0．01）;在糖尿病各年龄组中,胰岛素水平无明显变化（P〉0．05）;但血清C肽水平随年龄增大而升高,而胰岛素与C肽比值则显著下降（P〈0．01）。结论：测定血清C肽水平及胰岛素与C肽比值有助于了解糖尿病患者的肾功能状况,值得进一步深入探讨。     

妊高征患者血浆ET-1和血清INH、D-D检测的临床意义

目的:探讨了血浆内皮素-1(ET-1)和血清抑制素(INH)、D-二聚体(D-D)水平在妊高征患者发病中的变化和临床意义.方法:采用放射免疫分析、酶联法和生化法对32例妊高征患者进行了血浆ET-1和血清INH、D-D检测,并与35名正常孕妇作比较.结果:妊高征患者血浆ET-1和血清INH、D-D水平均非常显著地高于正常...     

颜色辨识状态下ERP时频特征与认知能力的相关性研究

研究不同程度认知能力人群的事件相关电位(ERP)的特征,从中提取出能有效识别血管性痴呆(VD)的电生理指标.选择正常青年组、VD组以及与VD同一年龄段的正常老年组作为被试人群,采用视觉通道诱发的oddball实验模式,对ERP信号运用平滑伪魏格纳分布(SPWVD)进行时频分析.通过不同被试人群的群延迟(GD)和瞬时频率(IF)的对比发现,大脑额、中央和顶区ERP信号的群延迟可反映3组不同被试之间认知能力的差异,为VD患者的早期诊断识别提供一个新的途径.