某保健食品胶囊减肥功能人体试食试验

目的了解某保健食品胶囊是否具有减肥作用。方法单纯性肥胖受试者100例,随机分为2组,试食组50例,对照组50例。试食组服用保健食品胶囊35 d,对照组不服用任何保健食品。所有对象均于试验开始及结束时均测试各项功效性指标。结果试食组在试用后体重平均下降2.45 kg,体脂总量平均下降1.69 kg,体脂百分率平均下降1.04%;皮下的脂肪厚度有2个测定位点减少,其中上臂减少了1.29 mm,脐旁3 cm处减少2.60 mm,腰围减少2.21 cm,与试食前比较,差异有统计学意义（P〈0.05）。对照组这些指标在试验前和后无差异。结论测试保健食品胶囊具有减肥功能。     

癞葡萄胶囊调节血糖人体试食试验的研究

癞葡萄 (MonordicacharantiaL .)为葫芦科苦瓜属植物 ,又名癞瓜、苦瓜。其癞葡萄的果实富含皂甙、多糖和多肽蛋白等生物活性物质 ,具有降血脂、降血糖的保健功能[1 ] 。1　材料与方法1 1　受试样品选用的癞葡萄鲜果 ,采收后经挑选去除坏、烂、虫蛀果后清洗。经沥干、切片、烘干、超微粉碎后灌装胶囊。1 2　试食方法在一所三级甲等医院的门诊 ,选择确诊的Ⅱ糖尿病病人69例作为受试对象 ,男性 34例 ,女性 35例 ,年龄范围为 30～78岁 ,受试者无严重心、肝、肾等并发症。受试对象随机分为试食组和对照组 ,各组内采用自身对照和两组间对照设计…     

芦荟胶囊通便功能的人体试食研究

[目的]观察芦荟胶囊的通便功能。[方法]采用双盲法将120例受试者按其便秘症状随机分为试食组和对照组，试食样品组服用受试样品，对照组服用与受试样品外观相似的安慰剂，连续服用7d后，观察两组排便次数、排便状况和粪便性状的变化。[结果]对照组试食前后排便次数分别为2．13和2．11次／周，排便状况积分分别为1．56和1．58分，粪便性状积分分别为1．79和1，64分，差别均无统计学意义；试食样品组试食前后排便次数分别为2．13和4．09次／周，排便状况积分分别为1．57和0．61分，粪便性状积分分别为1．83和0．63分，差别均有统计学意义（P〈0．01）；试食后试食样品组与对照组的排便次数、排便状况和粪便性状差别均有统计学意义（P〈0．01）。对照组和试食样品组试验前后未见不良反应，各项安全性指标均未见异常。[结论]芦荟胶囊对人体具有明显通便功能，且对机体健康无不良影响。     

辐照主副食品人体试食试验

在本所职工中选取43名(男性21名,女性22名)体检合格的志愿者分为试验组和对照组,采用双盲法分别食用经~(60)Coγ射线照射和未照射的主副食品,为期15周。试验组每日食用辐照主副食品占膳食总摄入量的62～71%,试验结果表明,受试者主观感觉良好,未出现不良体征和反应,体重略有增加,血常规和血、尿生化指标测定以及外周淋巴染色体畸变分析表明试食辐照主副食品对造血、肝肾功能、血脂血糖含量以及内分泌系统均无不良影响,对外周淋巴细胞染色体未见畸变效应。     

黄芪葛根胶囊降血糖作用的人体试食效果观察

目的 观察黄芪葛根胶囊对2型糖尿病患者辅助降血糖作用的有效性和安全性。方法 110例2型糖尿病患者,根据受试者的血糖水平随机分为试食组和对照组。试食组服用黄芪葛根胶囊60 d,对照组服用安慰剂。观察血糖变化及症状改善情况。结果 试食组试食前后空腹血糖值为(8.02±0.97) mmol/L和(7.11±0.78) mmol/L,对照组试食前后空腹血糖值为(7.94±0.94) mmol/L和(7.87±1.04) mmol/L;试食组试食前后餐后2 h血糖值为(12.36±1.27) mmol/L和(11.08±1.21) mmol/L,对照组试食前后餐后2 h血糖值为(12.24±1.16) mmol/L和(12.09±1.06) mmol/L。试食组空腹血糖及餐后2 h血糖分别下降10.96%和10.20%;试食组试食前后糖化血红蛋白分别为(7.86±0.64)%和(7.84±0.84)%,自身前后比较无显著差异。试食后主要临床症状,试食组均有明显改善;安全性指标试食前后无明显变化,未观察到明显的不良反应及过敏症状。结论 黄芪葛根胶囊对2型糖尿病患者具有辅助降糖作用。     

辐照蘑菇人体试食试验

以双盲法~(60)Co辐照蘑菇(吸收剂量为1.0KGY)对19名志愿者(男9名女10名)年龄21～40岁,进行了人体试食试验。将受试者按性别、年龄随机配对分成试验组和对照组,每日食用蘑菇250g,试验期28天,在此之前14天适应期食用非辐照蘑菇。辐照蘑菇在室温储存24小时后食用。试食期没有不良影响,对造血、肝、肾功能、血脂、血糖和内分泌功能试验组与对照相比两组没有显著性差异。外周淋巴细胞染色体畸变分析表明:试验组女性试食后染色体畸变率明显增加,而对照组与试食组于试食期后两组相比亦有明显差异。然而,染色体畸变率偏低,且均在正常范围内。同时,受试人数较少,因此所观察到的畸变率增加可能由于分析的系统误差所致,并非辐照蘑菇所致。     

某保健食品抗氧化功能人体试食试验

随着年龄的增长,自由基氧化造成人体器官和组织衰老,谷胱甘肽过氧化物酶、超氧化物歧化酶水平降低,过氧化脂质升高,某保健食品经抗氧化功能动物试验证实,对动物具有抗氧化功能的作用,为进一步验证其对人体的抗氧化作用,依据卫生部<保健食品检验与评价技术规范>2003年版,对该保健食品进行了为期3个月的人体试食试验研究.     

鲜河豚鱼人体试食试验的观察

我国的豚鱼资源比较丰富,大约有40余个品种,广泛分布于江河下游和近海区。河豚鱼虽有剧毒．但因其味道鲜美而深受渔民和沿海居民的喜好。我国(水产品管理办法)规定禁止鲜销,必须去头、去皮、去内脏盐腌晒干经检验无毒方可经销。近几年,国内近海河豚鱼养殖业在不断发展。为了更好地开发利用河豚鱼资源,我们就河豚鱼鱼种人群试食等方面开展研究。现报告如下。     

某保健食品改善营养性贫血功能人体试食试验

营养性贫血又称缺铁性贫血,是体内铁的储存不能满足正常红细胞生成需要而发生的贫血;是由于铁减少、需要量增加、铁利用障碍或丢失过多所致.形态学表现为小细胞低色素性贫血.某保健食品经动物试验证明具有改善营养性贫血功能,为进一步证实该保健食品对人体是否具有改善营养性贫血功能,依据卫生部<保健食品检验与评价技术规范>2003年版,对该保健食品进行了为期31 d的人体试食试验研究.     

Cariogenic bacteria as biomarkers for sugar intake

Cariogenic bacteria (lactobacilli and mutans streptococci) in saliva are possible biomarkers for sugar intake. We evaluated the strength of the evidence for an association between sugar intake and the two groups of bacteria, and concluded that there is a limited to moderately scientific basis for this association. Because of many confounding factors, bacteria counts can never be used as a precise measurement of sugar intake, but might still be useful on a group level.     

Association of free sugar intake with blood pressure and obesity measures in Australian adults

European Journal of Nutrition - This study examined the association of free sugar (FS) intake with obesity measures and blood pressure (BP) among a nationally representative sample of Australian...     

Dietary fat and the regulation of energy intake in human subjects

The role of dietary fat in the regulation of energy intake was assessed by manipulating a conventional diet and measuring spontaneous food consumption. Twenty-four women each consumed a sequence of three 2-wk dietary treatments in which 15-20%, 30-35%, or 45-50% of the energy was derived from fat. These diets consisted of foods that were similar in appearance and palatability but differed in the amount of high-fat ingredients used. Relative to their energy consumption on the medium-fat diet, the subjects spontaneously consumed an 11.3% deficit on the low-fat diet and a 15.4% surfeit on the high-fat diet (p less than 0.0001), resulting in significant changes in body weight (p less than 0.001). A small amount of caloric compensation did occur (p less than 0.02), which was greatest in the leanest subjects (p less than 0.03). These results suggest that habitual, unrestricted consumption of low-fat diets may be an effective approach to weight control.     

Empty calories? Nutrient intake in relation to sugar intake in English adolescents

The relation between the intake of added sugars and other nutrients was examined by analysing the diet of 405 English children aged 11–14 years. For each sex, the nutrient intake of the 30 subjects with the highest intake of added sugars in proportion to energy intake (g/MJ) was compared with the nutrient intake of the 30 subjects with the lowest intake of added sugars in proportion to energy intake. Those eating the highest amount of added sugars consumed less protein and vitamin D compared with those who ate the lowest amounts of added sugars.
Some differences in the nutrient intakes of the high and low added sugar groups were observed for girls only, and non-significant trends were observed in both sexes, tending to suggest that those consuming diets containing low levels of added sugars in proportion to energy (a low preference for sugar) ate a more nutrient-dense diet and, to some extent, supported the concept of 'empty calories'. However, when sugars intake was expressed as g weight (and not in proportion to energy), energy intake and consumption of most nutrients were considerably higher in those eating high levels of added sugars, probably because of their higher intake of foods in general.     

人外周静脉血血清蛋白质双向凝胶电泳条件优化

目的优化人外周静脉血血清蛋白质的双向凝胶电泳条件。方法血清总蛋白经去除白蛋白、IgG和丙酮沉淀去盐分处理后,固相pH梯度等电聚焦(IEF)和十二烷基硫酸钠-聚丙烯酰胺凝胶(SDS-PAGE)进行蛋白质电泳,对蛋白质的上样量、固相pH梯度(IPG)胶条的pH值范围、凝胶浓度、水化上样液体积和IEF程序及其参数等条件进行优化。结果将50～100μg蛋白质溶解于终体积为350μl水化上样液,应用pH4～7的IPG胶条和浓度12%的SDS-PAGE凝胶进行双向电泳,经优化电泳电流、时间等参数后,建立了合适的双向凝胶电泳条件,得到比较理想的蛋白质二维图谱。结论优化了血清蛋白质双向凝胶电泳条件,为进一步开展血清差异蛋白质组学研究奠定基础。     

Increasing fruit and vegetable intake and decreasing fat and sugar intake in families at risk for childhood obesity

OBJECTIVE: The goal of this study was to evaluate the effect of a parent-focused behavioral intervention on parent and child eating changes and on percentage of overweight changes in families that contain at least one obese parent and a non-obese child. RESEARCH METHODS AND PROCEDURES: Families with obese parents and non-obese children were randomized to groups in which parents were provided a comprehensive behavioral weight-control program and were encouraged to increase fruit and vegetable intake or decrease intake of high-fat/high-sugar foods. Child materials targeted the same dietary changes as their parents without caloric restriction. RESULTS: Changes over 1 year showed that treatment influenced targeted parent and child fruit and vegetable intake and high-fat/high-sugar intake, with the Increase Fruit and Vegetable group also decreasing their consumption of high-fat/high-sugar foods. Parents in the increased fruit and vegetable group showed significantly greater decreases in percentage of overweight than parents in the decreased high-fat/high-sugar group. DISCUSSION: These results suggest that focusing on increasing intake of healthy foods may be a useful approach for nutritional change in obese parents and their children.