壳聚糖护肤液促进大鼠伤口愈合的研究

目的 探讨壳聚糖护肤液促进伤口愈合的作用。 方法 在大鼠背部伤口模型上用药,对壳聚糖护肤液组（A组）、碱性成纤维细胞生长因子组（B组）、生理氯化钠溶液组（C组）进行两两对照,肉眼观察伤口愈合时间,并于伤后3、7、14 d,取皮肤修复组织行光镜观察。结果 A组愈合时间为（17.3 ± 1.35） d,B组为（18.2 ± 1.15） d,C组为（24.0 ± 1.37） d。A组与B组的愈合时间差异无统计学意义（P > 0.05）,A组、B组与C组的愈合时间差异有统计学意义（P < 0.05）;壳聚糖护肤液在伤口愈合的早期促进毛细血管芽和血管内皮细胞生成,中期促进成纤维细胞和胶原纤维生成,后期使胶原纤维排列有序。结论 壳聚糖护肤液具有较好的促进伤口愈合的作用。     

碳素光促进皮肤糜烂、溃疡与伤口愈合的疗效观察

碳素光是一种包含红外线、紫外线、磁脉冲等多种光谱的综合光,具有消炎、止痛、杀灭多种微生物、促进上皮细胞生长、促进创面愈合等作用^[1]。2004年3-6月,我们运用碳素光理疗仪治疗多种伴有糜烂、溃疡的皮肤病以及CO₂激光治疗术后创面和慢性皮肤溃疡,取得了较好的疗效,现报道如下。     

腐植酸钠治疗麻风性溃疡的疗效观察

为了扩大腐植酸钠治疗麻风性溃疡的临床观察,于一九七七年六月,我院接受了25例疗效观察的任务。由于我院溃疡病例较少,所以只观察了12例,现将结果报告于下: 临床资料一、病例情况: 我院现有住院病人195人,其中并发足底溃疡者12例,溃疡13个,全部列入观察对     

激光与伤口的愈合

CO_2激光可聚焦成0.1～0.2mm的光束作为光刀进行组织切割或切除。在血管丰富 的损害部位,普通手术刀可导致大量出血,而CO_2激光刀可封闭小血管、淋巴管及末梢神经,切口相对不出血,术后疼痛及肿胀较轻,恶性细胞播散的可能性亦较小。CO_2激光刀切割时可产生高于300℃的高温,在直接受激光作用的碳化区很薄,仅10～20μm,向外的     

大气压低温等离子体对大鼠急性化脓性感染伤口愈合的影响

目的 探索大气压低温等离子体(cold atmospheric plasma,CAP)对大鼠化脓性感染伤口愈合的影响.方法 用金黄色葡萄球菌构建大鼠急性化脓性感染伤口模型.采用kINPen Med系统生成稳定的CAP,对大鼠化脓性伤口进行不同治疗剂量的处理,根据处理时间分为30 s,60 s,90 s实验组,以不处理的...     

仙林钠治疗扁平疣临床疗效观察

扁平疣临床上治疗复杂而多样 ,我们应用仙林钠 (大连美罗大药厂生产 ,成分为泛昔洛韦 )口服治疗扁平疣取得了较好的疗效 ,并与应用板兰根冲剂口服组进行了对比 ,现报道如下。临床资料　门诊经临床确诊为扁平疣的患者 80例 ,采用随机 ,双盲 ,平行方式将其分为两组。治疗组 :40例 ,其中男 18例 ,女 2 2例。年龄 18～ 2 8岁 ,平均年龄 2 6.7岁。面部扁平疣 3 1例 ;面、手共患 9例。病程半个月～ 2年不等。对照组 40例 ,男 12例 ,女 2 8例。年龄 18～ 3 2岁 ,平均年龄 2 4.7岁。面部扁平疣 3 5例 ;面手部共患 5例。病程 1个月～ 4年不等。入表 1…     

头孢唑肟钠治疗单纯性淋病60例疗效观察

目的 :评价头孢唑肟钠治疗单纯淋病的临床疗效与安全性。方法 :在性病门诊确诊为单纯性淋病 1 2 0例患者 ,随机分为两组 :治疗组用头孢唑肟钠 2g分两侧臀部一次性肌肉注射 ;对照组用淋必治 4g分两侧臀部一次性肌肉注射。结果 :治疗组的治愈率为 90 % ,对照组的治愈率为 92 % ,两组治愈率比较差异无显著性 ( χ2 =0 1 ,P >0 0 5 )。结论 :头孢唑肟钠治疗单纯性淋病疗效好 ,安全性高 ,可供临床选用的药物。     

头孢哌酮舒巴坦钠治疗淋病临床观察

目的探讨注射用头孢哌酮舒巴坦钠治疗淋球菌感染的临床效果。方法将我院2010年11月至2013年11月共78例淋病患者随机分为2组治疗观察疗效和不良反应。结果治疗组痊愈35例,显效4例,总有效率为100%,对照组中痊愈25例,显效10例,无效4例,总有效率为89.74%,治疗组显著优于对照组(P0.05),结论注射用头孢哌酮舒巴坦钠治疗淋病临床疗效好、起效快、不良反应少具有有效性和安全性。     

明胶酶与皮肤伤口愈合

皮肤伤口愈合是一个复杂而有序的生物学过程.基质金属蛋白酶尤其是明胶酶（MMP-2与MMP-9）参与皮肤伤口愈合过程中的炎症反应、上皮与血管再生、组织重塑等阶段.通过了解明胶酶在皮肤伤口愈合过程中的表达及其调节机制,有望开发以明胶酶为靶向的促进皮肤伤口愈合的治疗药物和措施.     

明胶酶与皮肤伤口愈合

皮肤伤口愈合是一个复杂而有序的生物学过程.基质金属蛋白酶尤其是明胶酶(MMP-2与MMP-9)参与皮肤伤口愈合过程中的炎症反应、上皮与血管再生、组织重塑等阶段.通过了解明胶酶在皮肤伤口愈合过程中的表达及其调节机制,有望开发以明胶酶为靶向的促进皮肤伤口愈合的治疗药物和措施.     

银屑病发病与创伤愈合机制的比较

比较银屑病发病及创伤愈合的机制,包括免疫细胞、炎症反应、细胞因子、纤维化、角质形成细胞增殖和血管内皮细胞增殖等方面,认为发生银屑病同形反应现象源于二者共同的致炎因子,推测银屑病皮损区愈合能力高于正常皮肤,期望能拓展银屑病的研究思路。     

Optimal Fluence and Duration of Low-Level Laser Therapy for Efficient Wound Healing in Mice

BackgroundLow-level laser (light) therapy is a promising technology that stimulates healing, relieves pain and inflammation, and restores function in injured body parts. However, few studies have compared the effects of light-emitting diodes of different fluence levels or different treatment durations.ObjectiveHere, we investigated the effects of various fluence levels and treatment durations on wound closure in mice.MethodsFull-thickness wounds were created on the dorsal skin using an 8-mm diameter punch, and the wounds were irradiated at 1, 4, or 40 J/cm² for 5 consecutive days starting on day 1. To determine the optimal irradiation duration, wounds were irradiated at the most potent fluence of previous study for 5, 10, or 15 days. Photographic documentation, skin biopsies, and wound measurements were performed to compare the effects of different treatment parameters.ResultsThe most effective fluence level was 40 J/cm² at day 5, as determined by monitoring wound closure. There were no statistically significant differences in wound healing with different durations.ConclusionWe have shown that repeated exposure to low levels of light significantly stimulates wound healing in mice and demonstrated more efficient wound closure with certain fluences of 830 nm irradiation.     

TWEAK/Fn14信号在皮肤急性创伤愈合中的作用

皮肤创伤愈合由炎症、增生和重塑等几个连续且相互重叠的阶段组成,涉及多种细胞类型、细胞因子与细胞外基质间复杂的相互作用。肿瘤坏死因子样细胞凋亡弱诱导剂(tumor necrosis factor-like weak inducer of apoptosis,TWEAK)通过与其受体成纤维细胞生长诱导因子14(fibroblast growth factor-inducible 14,Fn14)结合促进炎症反应、调控细胞增殖、迁移、分化和血管形成,从而在皮肤创伤愈合中发挥作用。本文旨在回顾皮肤创伤愈合和TWEAK/Fn14信号通路方面的研究进展,探索TWEAK/Fn14信号在急性皮肤创伤愈合中的重要作用。     

Effects of Human Adipose-derived Stem Cells on Cutaneous Wound Healing in Nude Mice

Background

Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration.

Objective

To investigate the effects of human ASCs on the healing of cutaneous wounds in nude mice.

Methods

15-mm round full-thickness skin defects were generated on the back of BALB/c nude mice. The mice were divided into three groups for wound coverage: (i) human ASCs-populated collagen gel, (ii) human dermal fibroblasts-populated collagen gel, and (iii) collagen gel alone. Wound contraction was prevented with a splint method. Wound size was measured 10 days after injury. At 28 days histological analysis was performed.

Results

Both ASCs and dermal fibroblasts accelerated wound closure, but dermal fibroblasts were more effective than ASCs. At 28 days, the dermal portion of ASCs or dermal fibroblasts wound scars were thicker than collagen gel wound scars.

Conclusion

ASCs and dermal fibroblasts stimulate cutaneous wound healing and improve scar thickness.     

Keratinocyte-Like Cells Trans-Differentiated from Human Adipose-Derived Stem Cells,Facilitate Skin Wound Healing in Mice

BackgroundMesenchymal stem cells (MSCs) have been reported to promote wound healing in both animal models and human studies. Among MSCs, adipose-derived stem cells (ADSCs) can be easily harvested in large quantities.ObjectiveWe investigated whether skin wound healing in mice can be facilitated by keratinocyte-like cells differentiated from ADSCs (KC-ADSCs).MethodsFor the wound contraction and epithelialization model, a 20 mm×20 mm fullthickness skin wound was made on the dorsum. For the wound epithelialization model, a 6 mm×6 mm full-thickness skin wound was made on the dorsum. A nitrile rubber stent with an inner diameter of 8 mm was sutured around the wounds to minimize wound contraction. Undifferentiated ADSCs (uADSCs) or KC-ADSCs was injected around the wound base in both models. To evaluate whether the injected ADSCs could enhance wound contraction in a skin wound, the contractile activity of ADSCs was assessed by an in vitro type I collagen gel contraction assay. Alpha-smooth muscle actin (αSMA) expressions in uADSCs and KC-ADSCs were also evaluated by flow cytometry and real-time polymerase chain reaction.ResultsIn a wound contraction and epithelialization model, KC-ADSCs further facilitated wound healing compared with uADSCs. In a wound epithelialization model, KC-ADSCs also further facilitated wound epithelialization compared with uADSCs. The contractile activity of KC-ADSCs was lower than that of uADSCs. The uADSCs expressed high levels of αSMA, which decreased after the differentiation into keratinocyte-like cells.ConclusionOur results suggest that the wound healing effect of KC-ADSCs depends primarily on re-epithelialization rather than wound contraction.     

Research Advances in the Application of Adipose-Derived Stem Cells Derived Exosomes in Cutaneous Wound Healing

Cutaneous wound healing has always been an intractable medical problem for both clinicians and researchers, with an urgent need for more efficacious methods to achieve optimal outcomes morphologically and functionally. Stem cells, the body''s rapid response ‘road repair crew,’ being on standby to combat tissue injuries, are an essential part of regenerative medicine. Currently, the use of adipose-derived stem cells (ADSCs), a kind of mesenchymal stem cells with multipotent differentiation and self-renewal capacity, is surging in the field of cutaneous wound healing. ADSCs may exert influences either by releasing paracrine signalling factors or differentiating into mature adipose cells to provide the ‘building blocks’ for engineered tissue. As an important paracrine substance released from ADSCs, exosomes are a kind of extracellular vesicles and carrying various bioactive molecules mediating adjacent or distant intercellular communication. Previous studies have indicated that ADSCs derived exosomes (ADSCs-Exos) promoted skin wound healing by affecting all stages of wound healing, including regulating inflammatory response, promoting proliferation and migration of fibroblasts or keratinocytes, facilitating angiogenesis, and regulating remodeling of extracellular matrix, which have provided new opportunities for understanding how ADSCs-Exos mediate intercellular communication in pathological processes of the skin and therapeutic strategies for cutaneous wound repair. In this review, we focus on elucidating the role of ADSCs-Exos at various stages of cutaneous wound healing, detailing the latest developments, and presenting some challenges necessary to be addressed in this field, with the expectation of providing a new perspective on how to best utilize this powerful cell-free therapy in the future.     

A Histological Study of Cutaneous Thermal Wounds Using a Clostridium perfringens-Derived Wound Healing Substance with Wound Healing Stimulation Activity

We studied the effects of a Clostridium perfringens-derived wound-healing substance (WHS) on the healing of thermal burn wounds. Third-degree burn injuries were inflicted on the back skin of rats. We histologically evaluated the effects of WHS ointments and compared them with those of lysozyme chloride ointment. We observed the formation of dermal collagen fibers and the increase of capillaries in the WHS ointment treated groups. From the results of hematoxylin and eosin staining and silver staining, an increase in capillaries was observed one week after the application of WHS ointment. Three weeks after the application, when the epithelization was in the final stage, capillary formation ceased. In the WHS ointment-applied groups, electron microscopic observation showed that new collagen fibers were regularly formed in the dermis. On the other hand, in the lysozyme chloride ointment-applied groups, new collagen fibers were present, but were irregularly formed. The main wound healing stimulative action of the WHS ointment could be due to its acceleration of new capillary formation.     

Polydeoxyribonucleotides Improve Diabetic Wound Healing in Mouse Animal Model for Experimental Validation

BackgroundWound healing mechanisms is believed to have effects similar to wound healing disorders in diabetic patients, including abnormal inflammatory cells, angiogenesis disorders, and reduced collagen synthesis. Therefore, reestablishment of structural and promoted angiogenesis could be beneficial to promote wound healing process.ObjectiveTherefore, we investigated whether the polydeoxyribonucleotide (PDRN) that was self-production in Korea, could be useful as an intradermal injection for promoting wound healing. Also, we validate for wound healing effect of PDRN using healing-impaired (db/db) mice.MethodsIn this study, we confirmed the effects of PDRN by creating wound models in in vitro and in vivo model. Using an in vitro wound healing assay, we observed that PDRN stimulated closure of wounded monolayers of human fibroblast cells. PDRN (8.25 mg/ml) or phosphate-buffered saline (0.9% NaCl) was injected once daily into the dermis adjacent to the wound for 12 days after skin injury.ResultsTime course observations revealed that mice treated with PDRN showed accelerated wound closure and epidermal and dermal regeneration, enhanced angiogenesis. The wound area and depth decreased at 3, 6, 9, and 12 days after skin injury. Histological evaluation showed an increase of vascular endothelial growth factor, CD31, and collagen fibers in the PDRN group compared with the control group, indicating that PDRN was effective in the treatment of delayed wound healing caused by diabetes.ConclusionThis study suggests that our PDRN has a wound healing effect in transgenic animal models with cells and diabetes through angiogenesis.     

重组人表皮细胞生长因子与碱性成纤维细胞生长因子联合应用促进创面愈合

目的观察重组人表皮细胞生长因子与碱性成纤维细胞生长因子联合应用对慢性难愈性创面修复的效果。方法自2002年以来应用重组人表皮细胞生长因子联合碱性成纤维细胞生长因子对清创后的慢性难愈性创面局部喷涂,无菌纱布包扎。治疗后7、14、21、28天分别计算创面愈合数。结果14天内创面愈合14个,14～21天愈合4个,22～28天愈合3个;总有效率100%。结论重组人表皮细胞生长因子联合碱性成纤维细胞生长因子能明显促进慢性难愈性创面愈合。     

COMP-angiopoietin 1 Gene Transfer Enhances Cutaneous Wound Healing by Promoting Angiogenesis

Background

Angiogenesis is crucial for wound healing and exogenous supplements of the angiogenic growth factors have been known to promote cutaneous wound healing. Angiopoietin (Ang) 1 is a recently discovered angiogenic factor and there have been few studies of its effect on cutaneous wound healing.

Objective

We examined the effect of Ang 1 on cutaneous wound healing.

Methods

Cartilage oligomeric matrix protein (COMP)-Ang 1 (Ade-COMP-Ang 1)- was intravenously injected to rats two days before surgery creating full-thickness wounds. The clinical wound healing rate and the number of vessels in the skin samples were evaluated on days 3, 7 and 14 post operation.

Results

At post-operation day 3, 7 and 14, the clinical wound healing rate was 38.3%, 59.4% and 92.1%, respectively, in the Ade-COMP-Ang 1-treated group, compared with 20.5%, 47.5% and 87.3%, respectively, in the Ade-LacZ-treated group. There were significant differences in the results of day 3 and day 7 between two groups (p<0.05). Histopathologically, the number of the vessels of the Ade-COMP-Ang 1-treated group was 73.7, 94.1 and 62.7 at day 3, 7 and 14, compared with that of the Ade-LacZ-treated group, 53.5, 83.9, and 56.9. The differences in the results of the two groups were statistically significant (p<0.05).

Conclusion

These results indicate that Ade-COMP-Ang 1 therapy significantly accelerats wound healing by promoting angiogenesis. However, further study using Ade-COMP-Ang 1 gene therapy for chronic wounds in which the formation of new blood vessels is impaired is needed in the near future.